CN106754854A - A kind of granulating preparation method of deodorant complex micro organism fungicide - Google Patents
A kind of granulating preparation method of deodorant complex micro organism fungicide Download PDFInfo
- Publication number
- CN106754854A CN106754854A CN201611001677.2A CN201611001677A CN106754854A CN 106754854 A CN106754854 A CN 106754854A CN 201611001677 A CN201611001677 A CN 201611001677A CN 106754854 A CN106754854 A CN 106754854A
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- CN
- China
- Prior art keywords
- micro organism
- complex micro
- organism fungicide
- solution
- deodorant
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
- A61L9/012—Deodorant compositions characterised by being in a special form, e.g. gels, emulsions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
Abstract
The invention discloses a kind of granulating preparation method of deodorant complex micro organism fungicide; polyvinyl alcohol and sodium alginate are matched somebody with somebody to be carried out being configured to mixed liquor by prescribed concentration; and activated carbon, diatomite and sodium bentonite are added wherein, and then it is stirred and granulates, 2wt%CaCl is first put into after granulation2It is crosslinked with the mixed solution of 4wt% boric acid, is placed into the Na of 0.5mol/L2SO4Continue to be crosslinked in solution, be finally dried and preserved with normal temperature, obtain final product the immobilization particle of deodorant complex micro organism fungicide.The present invention combines the high intensity of polyvinyl alcohol and the high-moisture percentage of sodium alginate; the characteristics of particle after preparation has big elastic good, pliability and moisture content high; not only the activity that can guarantee that microorganism but also the shaping that can guarantee that granulating, while also solving the problems, such as that the deodorant of the form of liquid such as is storing, transports and using there is inconvenience at the aspect.
Description
Technical field
The present invention relates to biological fixnig and microbial environment protecting technical field, specially a kind of deodorant complex micro organism fungicide
Granulating preparation method.
Background technology
As economic continues to develop, the improvement of people's living standards, urban and rural residents to the standard of living environment increasingly
Height, stench directly affects the quality of life of people as one of environmental hazard, or even jeopardizes the health of people, increasingly
Paid close attention to by people.The essence of microbial deodorant is to be by some stink substance decomposition and inversions with the microorganism of specific function
The generation of the material or suppression stink substance of odorless removes the purpose of stink so as to reach, and biological deodorizing is due to without dirt
Dye, the features such as continuation is strong, oneself turns into one of focus of environmentalist's research.
At present, the deodorizing microorganism microbial inoculum of in the market mainly exists in fluid form, and it is being stored, transports and is using
Etc. aspect there is inconvenience, nor discharged for a long time beneficial to microbial bacterial agent, immobilized microorganism technique can make micro- life
Thing has density higher in a certain FX, and the loss of microorganism is mitigated or eliminated, and improves reaction speed, makes it in complexity
Efficient performance can be played consistently in environment and under fierce operating condition, polyvinyl alcohol is embedded as a kind of new microorganism
Fixation support, with high mechanical strength it is high, chemical property is good, do microorganism decomposability strong, nontoxic to microorganism, cheap
Etc. series of advantages, alginate is a kind of natural polysaccharide polymers extracted from the cell membrane of marine alga, and it has good
Biocompatibility and biodegradability, form simple gel, and with non-toxic and lower-cost feature, polyvinyl alcohol
Hydrophilic polymer is with sodium alginate, but it is all defective separately as microbial carrier material, and regarding to the issue above, spy carries
Go out a kind of granulating preparation method of deodorant complex micro organism fungicide.
The content of the invention
It is above-mentioned to solve it is an object of the invention to provide a kind of granulating preparation method of deodorant complex micro organism fungicide
The problem proposed in background technology.
To achieve the above object, the present invention provides following technical scheme：A kind of granulating of deodorant complex micro organism fungicide
Preparation method, comprises the following steps：
(1) 15g sodium alginates are weighed and 400g polyvinyl alcohol is dissolved in the water of 2L, boiling water bath stirring and dissolving, treat completely molten
Appropriate water is weighed after solution again to pour into, and boiling water bath stirring and dissolving, distinguish the ultimate density of polyvinyl alcohol and sodium alginate
It is 20% and 0.75%；
(2) by the alginic acid of 200g activated carbons, 200g diatomite, the polyvinyl alcohol of 1.5L20% concentration and 0.75% concentration
The mixed liquor of sodium is added to 600ml and is in the bacterial solution in logarithmic growth later stage, and uses mixer stirring and adsorbing
10min, is obtained microbial inoculum；
(3) 400g diatomite and 200g sodium bentonites are added in microbial inoculum obtained in step (2), and first
With mixer stirring at low speed 10min, then with mixer high-speed stirred 5min, it is made bacterial mixture；
(4) bacterial mixture that step (3) is obtained is granulated with comminutor, that is, obtains bacteria particles；
(5) 400g boric acid and 200gCaCl are weighed2, it is dissolved in 10L hot water, 2wt%CaCl is obtained2With 4wt% boric acid
Mixed solution；
(6) bacteria particles that step (4) is obtained are placed in and obtained in step (5) contain 2wt%CaCl2With 4wt% boron
It is crosslinked 4-6 hours in the mixed solution of acid；
(7) 710gNa is weighed2SO4It is dissolved in 10L hot water, the Na of 0.5mol/L is obtained2SO4Solution；
(8) bacteria particles after step (6) is crosslinked are transferred to the Na of 0.5mol/L obtained in step (7)2SO4In solution
Continue to be crosslinked 8-12 hours；
(9) bacteria particles in step (8) after crosslinking are taken out, is put into continuous drying 36-48 hour, drying in barbecue box
Normal temperature is preserved afterwards, obtains final product the immobilization particle of deodorant complex micro organism fungicide.
Preferably, bacteria particles are placed in and contain 2wt%CaCl in step (6)2It is crosslinked with the mixed solution of 4wt% boric acid
Time be 5 hours.
Preferably, Na of the bacteria particles in 0.5mol/L in step (8)2SO4Crosslinking time in solution is 10 hours.
Preferably, in step (9) temperature range of barbecue box in 30-40 degree.
Preferably, the time continuous drying in barbecue box in step (9) is 42 hours.
Compared with prior art, the beneficial effects of the invention are as follows：A kind of granulating of deodorant complex micro organism fungicide makes
Method, the high-moisture percentage of the high intensity of polyvinyl alcohol and sodium alginate is combined, and the particle after preparation has elasticity good, soft
The characteristics of toughness is greatly and moisture content is high, the activity that not only can guarantee that microorganism but also the shaping that can guarantee that granulating, while also solving
There is inconvenience the aspect such as storing, transport and use in the deodorant of the form of liquid.
Specific embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection
Enclose.
The present invention provides a kind of technical scheme：A kind of granulating preparation method of deodorant complex micro organism fungicide, including such as
Lower step：
(1) 15g sodium alginates are weighed and 400g polyvinyl alcohol is dissolved in the water of 2L, boiling water bath stirring and dissolving, treat completely molten
Appropriate water is weighed after solution again to pour into, and boiling water bath stirring and dissolving, distinguish the ultimate density of polyvinyl alcohol and sodium alginate
It is 20% and 0.75%；
(2) by the alginic acid of 200g activated carbons, 200g diatomite, the polyvinyl alcohol of 1.5L20% concentration and 0.75% concentration
The mixed liquor of sodium is added to 600ml and is in the bacterial solution in logarithmic growth later stage, and uses mixer stirring and adsorbing
10min, is obtained microbial inoculum；
(3) 400g diatomite and 200g sodium bentonites are added in microbial inoculum obtained in step (2), and first
With mixer stirring at low speed 10min, then with mixer high-speed stirred 5min, it is made bacterial mixture；
(4) bacterial mixture that step (3) is obtained is granulated with comminutor, that is, obtains bacteria particles；
(5) 400g boric acid and 200gCaCl are weighed2, it is dissolved in 10L hot water, 2wt%CaCl is obtained2With 4wt% boric acid
Mixed solution；
(6) bacteria particles that step (4) is obtained are placed in and obtained in step (5) contain 2wt%CaCl2With 4wt% boron
It is crosslinked 4-6 hours in the mixed solution of acid；
(7) 710gNa is weighed2SO4It is dissolved in 10L hot water, the Na of 0.5mol/L is obtained2SO4Solution；
(8) bacteria particles after step (6) is crosslinked are transferred to the Na of 0.5mol/L obtained in step (7)2SO4In solution
Continue to be crosslinked 8-12 hours；
(9) bacteria particles in step (8) after crosslinking are taken out, is put into continuous drying 36-48 hour, drying in barbecue box
Normal temperature is preserved afterwards, obtains final product the immobilization particle of deodorant complex micro organism fungicide.
Bacteria particles are placed in and contain 2wt%CaCl in step (6)2With the time being crosslinked in the mixed solution of 4wt% boric acid
It is 5 hours, Na of the bacteria particles in 0.5mol/L in step (8)2SO4Crosslinking time in solution is 10 hours, in step (9)
The temperature range of barbecue box continuous drying in the barbecue box time in 30-40 degree, step (9) is 42 hours.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (5)
1. a kind of granulating preparation method of deodorant complex micro organism fungicide, it is characterised in that：Comprise the following steps：
(1) 15g sodium alginates are weighed and 400g polyvinyl alcohol is dissolved in the water of 2L, boiling water bath stirring and dissolving, until completely dissolved
Appropriate water is weighed again to pour into, and boiling water bath stirring and dissolving, it is respectively polyvinyl alcohol and the ultimate density of sodium alginate
20% and 0.75%；
(2) by the sodium alginate of 200g activated carbons, 200g diatomite, the polyvinyl alcohol of 1.5L20% concentration and 0.75% concentration
Mixed liquor is added to 600ml and is in the bacterial solution in logarithmic growth later stage, and with mixer stirring and adsorbing 10min, makes
Obtain microbial inoculum；
(3) 400g diatomite and 200g sodium bentonites are added in microbial inoculum obtained in step (2), and first with stirring
Machine stirring at low speed 10min is mixed, then with mixer high-speed stirred 5min, is made bacterial mixture；
(4) bacterial mixture that step (3) is obtained is granulated with comminutor, that is, obtains bacteria particles；
(5) 400g boric acid and 200gCaCl are weighed2, it is dissolved in 10L hot water, 2wt%CaCl is obtained2With the mixing of 4wt% boric acid
Solution；
(6) bacteria particles that step (4) is obtained are placed in and obtained in step (5) contain 2wt%CaCl2It is mixed with 4wt% boric acid
It is crosslinked 4-6 hours in conjunction solution；
(7) 710gNa is weighed2SO4It is dissolved in 10L hot water, the Na of 0.5mol/L is obtained2SO4Solution；
(8) bacteria particles after step (6) is crosslinked are transferred to the Na of 0.5mol/L obtained in step (7)2SO4Continue in solution
Crosslinking 8-12 hours；
(9) bacteria particles in step (8) after crosslinking are taken out, be put into it is continuous drying 36-48 hour in barbecue box, it is normal after drying
Temperature is preserved, and obtains final product the immobilization particle of deodorant complex micro organism fungicide.
2. the granulating preparation method of a kind of deodorant complex micro organism fungicide according to claim 1, it is characterised in that：Step
Suddenly bacteria particles are placed in and contain 2wt%CaCl in (6)2It it is 5 hours with the time being crosslinked in the mixed solution of 4wt% boric acid.
3. the granulating preparation method of a kind of deodorant complex micro organism fungicide according to claim 1, it is characterised in that：Step
Suddenly Na of the bacteria particles in 0.5mol/L in (8)2SO4Crosslinking time in solution is 10 hours.
4. the granulating preparation method of a kind of deodorant complex micro organism fungicide according to claim 1, it is characterised in that：Step
Suddenly in (9) temperature range of barbecue box in 30-40 degree.
5. the granulating preparation method of a kind of deodorant complex micro organism fungicide according to claim 1, it is characterised in that：Step
Suddenly the time continuous drying in barbecue box in (9) is 42 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611001677.2A CN106754854A (en) | 2016-11-11 | 2016-11-11 | A kind of granulating preparation method of deodorant complex micro organism fungicide |
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| CN201611001677.2A CN106754854A (en) | 2016-11-11 | 2016-11-11 | A kind of granulating preparation method of deodorant complex micro organism fungicide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107352657A (en) * | 2017-07-21 | 2017-11-17 | 轻工业环境保护研究所 | A kind of Zero-valent Iron biology carbon source composite drug for in-situ immobilization chlorohydrocarbon polluted underground water |
| CN109609406A (en) * | 2018-12-27 | 2019-04-12 | 广州市金龙峰环保设备工程股份有限公司 | A kind of slow-release microbial bacterial agent and preparation method thereof administered for black and odorous water |
| CN110182957A (en) * | 2019-04-29 | 2019-08-30 | 上海理工大学 | A kind of multifunctional ecological chinampa of Solar use |
| CN110368513A (en) * | 2019-07-16 | 2019-10-25 | 成都新柯力化工科技有限公司 | A kind of deodorant and preparation method for toilet |
| CN111470615A (en) * | 2020-05-06 | 2020-07-31 | 北京工业大学 | Preparation and application of sulfate-reducing-reinforced composite bacteria embedded bioactive filler |
| CN111484134A (en) * | 2020-05-06 | 2020-08-04 | 北京工业大学 | Preparation and application of denitrification embedded biological annular active filler |
| CN115090106A (en) * | 2022-06-28 | 2022-09-23 | 广东中微环保生物科技有限公司 | Biological purification particle, preparation method thereof and purification device containing biological purification particle |
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| CN104894099A (en) * | 2015-06-12 | 2015-09-09 | 福建省农业科学院中心实验室 | Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles |
| CN105000921A (en) * | 2015-05-13 | 2015-10-28 | 黑龙江八一农垦大学 | Modified zeolite for immobilizing ammonia removal fungus strain |
| CN105018458A (en) * | 2015-07-31 | 2015-11-04 | 湖南大学 | Compound microbial agent as well as preparation method and application thereof |
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| CN101358185A (en) * | 2008-09-19 | 2009-02-04 | 哈尔滨理工大学 | Method for fixing nitrile hydratase strain by sodium alginate-polyvinyl alcohol |
| CN105000921A (en) * | 2015-05-13 | 2015-10-28 | 黑龙江八一农垦大学 | Modified zeolite for immobilizing ammonia removal fungus strain |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107352657A (en) * | 2017-07-21 | 2017-11-17 | 轻工业环境保护研究所 | A kind of Zero-valent Iron biology carbon source composite drug for in-situ immobilization chlorohydrocarbon polluted underground water |
| CN107352657B (en) * | 2017-07-21 | 2023-09-19 | 北京市科学技术研究院资源环境研究所 | Zero-valent iron biological carbon source composite agent for in-situ remediation of chlorinated hydrocarbon polluted groundwater |
| CN109609406A (en) * | 2018-12-27 | 2019-04-12 | 广州市金龙峰环保设备工程股份有限公司 | A kind of slow-release microbial bacterial agent and preparation method thereof administered for black and odorous water |
| CN110182957A (en) * | 2019-04-29 | 2019-08-30 | 上海理工大学 | A kind of multifunctional ecological chinampa of Solar use |
| CN110368513A (en) * | 2019-07-16 | 2019-10-25 | 成都新柯力化工科技有限公司 | A kind of deodorant and preparation method for toilet |
| CN111470615A (en) * | 2020-05-06 | 2020-07-31 | 北京工业大学 | Preparation and application of sulfate-reducing-reinforced composite bacteria embedded bioactive filler |
| CN111484134A (en) * | 2020-05-06 | 2020-08-04 | 北京工业大学 | Preparation and application of denitrification embedded biological annular active filler |
| CN111484134B (en) * | 2020-05-06 | 2022-03-22 | 北京工业大学 | Preparation and application of denitrification embedded biological annular active filler |
| CN115090106A (en) * | 2022-06-28 | 2022-09-23 | 广东中微环保生物科技有限公司 | Biological purification particle, preparation method thereof and purification device containing biological purification particle |
| CN115090106B (en) * | 2022-06-28 | 2023-09-12 | 广东中微环保生物科技有限公司 | Biological purification particle, preparation method thereof and purification device containing particle |
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