CN106754512A - One plant of orthoresol degradation bacteria and its application - Google Patents
One plant of orthoresol degradation bacteria and its application Download PDFInfo
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- CN106754512A CN106754512A CN201611200249.2A CN201611200249A CN106754512A CN 106754512 A CN106754512 A CN 106754512A CN 201611200249 A CN201611200249 A CN 201611200249A CN 106754512 A CN106754512 A CN 106754512A
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Abstract
The invention discloses one plant of orthoresol degradation bacteria and its application.One plant of orthoresol degradation bacteria TJKYLJF 01 provided by the present invention belongs to pseudomonas (Pseudomonas sp.), and deposit number is CGMCC NO.10177.The bacterium is gram-positive bacteria, bacterium colony is white, neat in edge, it is rounded, smooth moistening, central protuberance, using orthoresol growth and breeding can be carried out as sole carbon source, nitrogen source and the energy, it is in 48h that the orthoresol degraded of 1000mg/L is complete, and it is resistant to the orthoresol at concentrations up to 1500mg/L, there is excellent degradation effect to higher concentration orthoresol industrial wastewater, the bacterium can be applied to the biological reinforced treatment of coking chemical waste water or coal chemical industrial waste water or petrochemical wastewater, have a extensive future.
Description
Technical field
The present invention relates to microorganism and biodegradation technique field, in particular it relates to one plant of orthoresol degradation bacteria and its should
With the especially application in coking, Coal Chemical Industry, petrochemical wastewater treatment.
Background technology
Orthoresol is also known as o-cresol, ortho-methyl phenol, 2- methylphenols, 2- hydroxyl -1- toluene, 2- cresols, adjacent hydroxyl
Toluene；Orthoresol is second isomers of cresols.Orthoresol is mainly used as synthetic resin, it may also be used for make agricultural chemicals diformazan four
Chloroacetamide herbicide, disinfectant pharmaceutically, spices and chemical reagent and antioxidant etc., downstream product mainly have synthetic resin neighbour's first
Phenol phenolic resin, ortho-homosalicylic acid, to chlorine o-cresol, salicylaldhyde, Carvacrol and antioxidant
Deng.In addition can be additionally used in diluent, disinfectant and plasticizer of decanedioic acid production etc.
Orthoresol is typical benzene compounds containing phenol, is widely present in coal tar, coal gas and oil.Orthoresol has
Phenol smell, it is poisonous, it is corrosive, can cause systemic toxicity profiles after being sucked through respiratory tract.Orthoresol is difficult to biodegradation, easily passes through
Coking, Coal Chemical Industry, the outer pollution discharging ecological environment of petrochemical wastewater, have huge potential hazard to human health.
In wastewater treatment process, biological treatment has many advantages than physico-chemical process, and such as processing cost is relatively low, condition temperature
With do not produce secondary pollution etc..However, traditional biologic treating technique is extremely difficult to ring in some intractable industrial wastewaters
Guaranteed request, conventional activated sludge is at one's wit's end to the hard-degraded substance in waste water such as pyridine, quinoline, naphthalene, phenols etc..In recent years
Come, biological reinforcing technology improves original activated sludge to target stains by adding microorganisms with specific functions in activated sludge
The removal efficiency of thing, substantially increases sewage treatment capacity, thus increasingly causes the attention of people.
At present, some scholars are had both at home and abroad filter out the bacterial strain to orthoresol with efficient degradation performance, such as false unit cell
Bacterium (Pseudomonas sp.), thiobacilli (Thiobacillus), paracoccus (Paracoccus sp.) etc., these
Research only rests on laboratory stage mostly.Up to the present, there are some scholars to study both at home and abroad and show pseudomonad
(Pseudomonas sp.) can degrade various aromatic compounds such as Sodium Benzoate, pyridine and Polychlorinated biphenyls, but have no that its is degraded
The report of orthoresol.Containing certain density orthoresol in coking chemical waste water, coal chemical industrial waste water and petrochemical wastewater, and orthoresol
Belong to bio-refractory organic matter, therefore only have to orthoresol by the ordinary activated sludge in biological treatment link is difficult to realize
Effect degraded.
The content of the invention
It is an object of the invention to make up defect of the prior art, there is provided one plant of orthoresol degradation bacteria, realize that it is focused
Change the biological reinforced treatment of waste water, coal chemical industrial waste water and petrochemical wastewater.
Orthoresol degradation bacteria TJKYLJF-01 provided by the present invention comes from the soil on Treatment of Coking Effluent station periphery.With neighbour
Cresols carries out growth and breeding as sole carbon source, nitrogen source and the energy, to the flora in soil in orthoresol minimal medium
Domestication culture is carried out, and is isolated and purified by plate streaking and is obtained.
Orthoresol degradation bacteria provided by the present invention is gram-positive bacteria, and bacterium colony is white, and neat in edge is rounded,
Smooth moistening, central protuberance.Pseudomonad (Pseudomonas sp.) is accredited as through 16S rRNA, TJKYLJF-01 is named.Should
Bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 15th, 2014 (referred to as
CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101), protect
It is CGMCC No.10177 to hide numbering, entitled pseudomonad (Pseudomonas sp.) of classifying
The condition of culture of orthoresol degradation bacteria TJKYLJF-01 provided by the present invention is to be inoculated in culture medium,
Temperature is 25-35 DEG C, and initial pH is 7-9, and inoculum concentration is 1-3%, and shaking speed is 100-150rpm.
The formula of the culture medium is, K2HPO4·12H2O 2.24g, KH2PO42.74g, (NH4)2SO41g, MgCl2·
6H2O 0.2g, NaCl 0.1g, FeCl3·6H2O 0.02g, CaCl20.01g, 2mL trace element storing solution, supply distilled water
To 1000mL；The micro- storing solution component is MnSO4·H2O 0.1g, ZnSO4·7H2O 0.12g, H3BO30.07g,
Na2MoO4·H2O 0.04g, CuSO4·5H2O 0.02g, CoCl20.04g, supplies distilled water to 1000mL.
Orthoresol degradation bacteria TJKYLJF-01 of the invention is resistant to the orthoresol of concentration≤1500mg/L.
Microbial inoculum containing orthoresol degradation bacteria TJKYLJF-01 of the present invention belongs to protection scope of the present invention.
Microbial inoculum the invention provides orthoresol degradation bacteria TJKYLJF-01 or its zymotic fluid or containing it is in the adjacent first of degraded
Application in phenol.
In above-mentioned application, concentration≤1500mg/L of the control orthoresol in system；Optium concentration is 1000mg/L；
The condition of work of degradation process is：15-40 DEG C of temperature, most preferably preferably 25-35 DEG C, 30 DEG C；PH value 5-9, preferably
PH value 7-8.
In degradation process, if vibration degraded system, such as when vibrating rotating speed for 100-150r/min, orthoresol drop can be met
Solution bacterium TJKYLJF-01 improves TJKYLJF-01 bacterium to orthoresol degradation efficiency to the demand of dissolved oxygen.
Microbial inoculum the invention provides orthoresol degradation bacteria TJKYLJF-01 or its zymotic fluid or containing it is in purified industrial
Application in waste water.
Described industrial wastewater is the one kind in coking chemical waste water, coal chemical industrial waste water and petrochemical wastewater.
Orthoresol degradation bacteria TJKYLJF-01 of the invention can be carried out using orthoresol as sole carbon source, nitrogen source and the energy
Growth and breeding, it is in 48h that the orthoresol degraded of 1000mg/L is complete.
Microbial inoculum the invention provides orthoresol degradation bacteria TJKYLJF-01 or its zymotic fluid or containing it is in clean environment
In application.
Orthoresol degradation bacteria TJKYLJF-01 of the invention can be carried out using orthoresol as sole carbon source, nitrogen source and the energy
Growth and breeding, it is in 48h that the orthoresol degraded of 1000mg/L is complete.It is high that orthoresol degradation bacteria TJKYLJF-01 is resistant to concentration
Up to the orthoresol of 1500mg/L, there is excellent degradation effect to higher concentration orthoresol industrial wastewater, the bacterium can be applied to Jiao
Change the biological reinforced treatment of waste water, coal chemical industrial waste water and petrochemical wastewater, have a extensive future.
Brief description of the drawings
Growths and orthoresol degradation effect graph of a relation of the Fig. 1 for orthoresol degradation bacteria TJKYLJF-01.
Fig. 2 is influence figure of the orthoresol initial concentration to orthoresol degradation bacteria TJKYLJF-01 degraded orthoresols.
Fig. 3 is influence of the cultivation temperature to orthoresol degradation bacteria TJKYLJF-01 degraded orthoresols.
Fig. 4 is influence figure of the initial pH value to orthoresol degradation bacteria TJKYLJF-01 degraded orthoresols.
Fig. 5 is influence figure of the inoculum concentration to orthoresol degradation bacteria TJKYLJF-01 degraded orthoresols.
Fig. 6 is influence figure of the shaking speed to orthoresol degradation bacteria TJKYLJF-01 degraded orthoresols.
Fig. 7 is orthoresol degradation bacteria TJKYLJF-01 to adding the intensive treatment effect of the coking chemical waste water of higher concentration orthoresol
Fruit is schemed.
Fig. 8 is orthoresol degradation bacteria TJKYLJF-01 to adding the intensive treatment of the coal chemical industrial waste water of higher concentration orthoresol
Design sketch.
Fig. 9 is orthoresol degradation bacteria TJKYLJF-01 at the reinforcing for adding the petrochemical wastewater of higher concentration orthoresol
Reason design sketch.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.Without departing substantially from spirit of the invention
In the case of essence, the modification or replacement made to the inventive method, step or condition belong to the scope of the present invention.
If not specializing, chemical reagent used is conventional commercial reagent in embodiment, skill used in embodiment
The conventional meanses that art means are well known to those skilled in the art.
The separation of the orthoresol degradation bacteria TJKYLJF-01 of embodiment 1, identification and its to the degradation property of orthoresol
1st, the separation of bacterial strain, purifying
(1) strain source
Bacterium source picks up from the soil on Treatment of Coking Effluent station periphery, in dark brown, gathers the pedotheque 6 away from top layer 4-8cm
Part.
(2) bacterial strain is isolated and purified
The soil sample that will be gathered adds that sterile distilled water is empty to expose 48h, 10mL supernatants are taken after standing is inoculated in 90mL and contain
In the LB culture mediums (peptone 1%, sodium chloride 1%, yeast extract 0.5%) of 300mg/L orthoresols, at 30 DEG C and 150r/
Min activation cultures 24h.Take 5mL nutrient solutions to be forwarded in the minimal medium containing orthoresol 300mg/L, similarity condition domestication
Culture 2-3d, then takes 5mL nutrient solutions transposing to fresh orthoresol minimal medium, and so repeatedly 3 times, wherein orthoresol is dense
Degree respectively 300,500,1000mg/L；Domestication liquid containing orthoresol 1000mg/L is diluted by different proportion, is respectively coated on
On orthoresol inorganic salts flat board containing orthoresol 500mg/L, 30 DEG C of culture 3d.Sharp-edged bacterium colony is selected, is drawn through LB flat boards
Line is purified to acquisition single bacterium colony, and orthoresol Degrading experiment is carried out respectively.After determining each strains for degrading orthoresol ability, with containing adjacent first
The excellent bacterial strain of the LB slant preservation degradation properties of phenol 500mg/L.
2nd, the identification of bacterial strain
(1) colony morphology characteristic and physio-biochemical characteristics of orthoresol degradation bacteria TJKYLJF-01
Orthoresol degradation bacteria TJKYLJF-01 bacterium colonies are white, and neat in edge is rounded, smooth moistening, central protuberance.
(2) the 16S rRNA identifications of orthoresol degradation bacteria TJKYLJF-01
The 16S rRNA genes of orthoresol degradation bacteria TJKYLJF-01 are cloned, is sequenced, then entered in GenBank
Row Blast is compared.Result shows, reaching 99% with its sequence similarity is pseudomonad (Pseudomonas sp.), its
16S rDNA sequences are as shown in SEQ ID NO.1.With reference to the physio-biochemical characteristics of bacterial strain, pseudomonad is belonged to
(Pseudomonas sp.), numbering is TJKYLJF-01.
During the bacterial strain was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on December 15th, 2014
The heart (abbreviation CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode
100101), deposit number is CGMCC No.10177, entitled pseudomonad (Pseudomonas sp.) of classifying.
3rd, the preparation of bacteria suspension
In LB culture mediums, 30 DEG C, 150rpm culture 24h take 5mL cultures and are connected to fresh LB trainings the ring microbionation of picking one
Support base, 30 DEG C of cultures 7-8h (bacterium be in exponential phase), 5000rpm, 10min are collected by centrifugation thalline, and with orthoresol without
Machine salt culture medium (pH 7.5) is washed 3 times, is resuspended in orthoresol minimal medium, adjusts cell density to OD600=1,4 DEG C
Save backup.
4th, the growth to orthoresol degradation bacteria TJKYLJF-01 and the relation of its degradation effect to orthoresol
Orthoresol degradation bacteria TJKYLJF-01 is inoculated in the minimal medium containing 500mg/L orthoresols, in 30 DEG C,
150r/min shaking table cultures, the growth of METHOD FOR CONTINUOUS DETERMINATION cell and the residual concentration of orthoresol are as shown in figure 1, measurement result shows：
The growth tendency of orthoresol degradation bacteria TJKYLJF-01 and the degradation process basic synchronization to orthoresol, the bacterium can utilize orthoresol
Promote own growth as sole carbon, nitrogen and energy substance, and have good degradation effect to orthoresol.
5th, influence of the condition of culture to the ability of the growth and its degraded orthoresol of orthoresol degradation bacteria TJKYLJF-01
(1) influence of orthoresol initial concentration：Investigate different orthoresol initial concentration (100,300,500,800,
1000th, 1500mg/L) to orthoresol degradation bacteria TJKYLJF-01 degrade orthoresol influence, orthoresol in METHOD FOR CONTINUOUS DETERMINATION nutrient solution
Residual concentration as shown in Fig. 2 orthoresol degradation bacteria TJKYLJF-01 can degrade the orthoresol of 1000mg/L in 48h
Entirely, with the raising of orthoresol initial concentration, the deadtime needed for degradation process is obviously prolonged, when o-cresol concentration reaches
During 1500mg/L, removals of the orthoresol degradation bacteria TJKYLJF-01 to orthoresol becomes very slow.Result shows, as orthoresol is dense
The rising of degree, Inhibition of degradation effect of the orthoresol to orthoresol degradation bacteria TJKYLJF-01 gradually strengthens.
(2) influence of cultivation temperature：Different cultivation temperatures (15,20,25,30,35,40 DEG C) are investigated to degrade orthoresol
The influence of bacterium TJKYLJF-01 degraded orthoresols, the residual concentration of orthoresol is as shown in figure 3, at 30 DEG C in METHOD FOR CONTINUOUS DETERMINATION nutrient solution
Cultivated by 48h, about 1000mg/L orthoresols only remnants 5.3mg/L, hence it is evident that better than the removal effect of other temperature.Experimental result
Show, the temperature comparisons of orthoresol degradation bacteria TJKYLJF-01 degraded orthoresols are 30 DEG C of 25 DEG C of ＞, 35 DEG C of ＞, 40 DEG C of ＞, 20 DEG C of ＞
15 DEG C of ＞.
(3) influence of initial pH：Different initial pH values (3,4,5,6,7,8,9,10) are investigated to orthoresol degradation bacteria
The influence of TJKYLJF-01 degraded orthoresols, the residual concentration of orthoresol is as shown in figure 4, according to degraded in METHOD FOR CONTINUOUS DETERMINATION nutrient solution
During the size of orthoresol residual concentration determine orthoresol degradation effect under different initial pH values：PH8 ≈ pH7 ＞ pH9 ＞ pH6
＞ pH5 ＞ pH4 ＞ pH3.
(4) influence of inoculum concentration：Investigate different inoculum concentration (0.5vol%, 2vol%, 5vol%, 10vol%,
15vol%, 20vol%) orthoresol of being degraded to orthoresol degradation bacteria TJKYLJF-01 influence, adjacent first in METHOD FOR CONTINUOUS DETERMINATION nutrient solution
The residual concentration of phenol is as shown in Figure 5, it can be seen that inoculum concentration is bigger, and orthoresol degradation rate is faster, when inoculum concentration is
During 10vol% and 15vol%, the removal rate highest of orthoresol in shaking flask shows that to reach 20vol% now single again when inoculum concentration
It is pure unobvious by influence of the inoculum concentration to improving orthoresol degradation effect is increased.
(5) influence of shaking speed：Investigate different shaking speed (50,100,150,200,250rpm) orthoresol is dropped
The influence of solution bacterium TJKYLJF-01 degraded orthoresols, the residual concentration of orthoresol is as shown in fig. 6, rotating speed in METHOD FOR CONTINUOUS DETERMINATION nutrient solution
It is the orthoresol degradation rate of 50r/min significantly lower than the degradation rate that rotating speed is 100,150,200 and 250r/min.In addition, experiment
Result shows that influence of the latter three to orthoresol degradation rate be not fairly obvious, shows when shaking speed is 100-150r/min
Demands of the orthoresol degradation bacteria TJKYLJF-01 to dissolved oxygen can have been met, it is now simple to be dropped to improving orthoresol by increasing speed
The influence for solving effect is not notable.
Intensive treatments of the orthoresol degradation bacteria TJKYLJF-01 of embodiment 2 to the coking chemical waste water of addition higher concentration orthoresol
In conical flask, the isolated orthoresol degradation bacteria TJKYLJF-01 of embodiment 1 is added to treatment coking and is given up
In the activated sludge of water, 3 groups of experiments are set and investigate its removal effect to orthoresol.
(a) 32mL coking chemical waste water+33mL orthoresol minimal medium+1000mg/L orthoresol+30mL activated sludge+
The orthoresol degradation bacteria TJKYLJF-01 bacteria suspensions of 5vol%；
After (b) 32mL coking chemical waste water+33mL orthoresol minimal medium+1000mg/L orthoresol+30mL autoclavings
Activated sludge+5vol% orthoresol degradation bacteria TJKYLJF-01 bacteria suspensions；
(c) 32mL coking chemical waste water+38m orthoresol minimal medium+1000mg/L orthoresol+30mL activated sludge.In
30 DEG C, 150rpm shaking table cultures.
As a result the residual concentration of orthoresol as shown in fig. 7, show in METHOD FOR CONTINUOUS DETERMINATION nutrient solution：Orthoresol degradation bacteria is not added
The activated sludge of TJKYLJF-01 is slow to the orthoresol degraded in waste water, adds orthoresol degradation bacteria TJKYLJF-01 then notable
Degradation capability of the activated sludge to orthoresol is improve, and will contain about the coking chemical waste water of 1000mg/L orthoresols in 48h
Orthoresol is removed completely.
The orthoresol degradation bacteria TJKYLJF-01 of embodiment 3 to add higher concentration orthoresol coal chemical industrial waste water reinforcing at
Reason
In conical flask, orthoresol degradation bacteria TJKYLJF-01 is added in the activated sludge for the treatment of coal chemical industrial waste water,
3 groups of experiments are set and investigate its removal effect to orthoresol.
(a) 32mL coal chemical industrial waste water+33mL orthoresol minimal medium+1000mg/L orthoresol+30mL activated sludge+
The orthoresol degradation bacteria TJKYLJF-01 bacteria suspensions of 5vol%；
(b) 32mL coal chemical industrial waste water+33mL orthoresol minimal medium+1000mg/L orthoresol+30mL autoclavings
The orthoresol degradation bacteria TJKYLJF-01 bacteria suspensions of activated sludge+5vol% afterwards；
(c) 32mL coal chemical industrial waste water+38m orthoresol minimal medium+1000mg/L orthoresol+30mL activated sludge.
In 30 DEG C, 150rpm shaking table cultures.
As a result the residual concentration of orthoresol as shown in figure 8, show in METHOD FOR CONTINUOUS DETERMINATION nutrient solution：Orthoresol degradation bacteria
TJKYLJF-01 serves obvious bioaugmentation, and the coal chemical industrial waste water of 1000mg/L orthoresols is will contain about in 48h
In orthoresol remove completely.
Reinforcings of the orthoresol degradation bacteria TJKYLJF-01 of embodiment 4 to the petrochemical wastewater of addition higher concentration orthoresol
Treatment
In conical flask, orthoresol degradation bacteria TJKYLJF-01 is added to the activated sludge for the treatment of petrochemical wastewater
In, 3 groups of experiments are set and investigate its removal effect to orthoresol.
A () 32mL petrochemical wastewater+33mL orthoresol minimal medium+1000mg/L orthoresols+30mL activity is dirty
The orthoresol degradation bacteria TJKYLJF-01 bacteria suspensions of mud+5vol%；
B () 32mL petrochemical wastewater+33mL orthoresol minimal medium+1000mg/L orthoresol+30mL high pressures are gone out
The orthoresol degradation bacteria TJKYLJF-01 bacteria suspensions of the activated sludge+5vol% after bacterium；
C () 32mL petrochemical wastewater+38m orthoresol minimal medium+1000mg/L orthoresols+30mL activity is dirty
Mud.In 30 DEG C, 150rpm shaking table cultures.
As a result the residual concentration of orthoresol as shown in figure 9, show in METHOD FOR CONTINUOUS DETERMINATION nutrient solution：Orthoresol degradation bacteria
TJKYLJF-01 serves obvious bioaugmentation, and the coal chemical industrial waste water of 1000mg/L orthoresols is will contain about in 48h
In orthoresol remove completely.
The orthoresol degradation bacteria TJKYLJF-01 of this specific embodiment can by the use of orthoresol as sole carbon source, nitrogen source and
The energy carries out growth and breeding, in 48h that the orthoresol degraded of 1000mg/L is complete.Orthoresol degradation bacteria TJKYLJF-01 is resistant to
By the orthoresol at concentrations up to 1500mg/L, there is excellent degradation effect to higher concentration orthoresol industrial wastewater, the bacterium can
The biological reinforced treatment of coking chemical waste water, coal chemical industrial waste water and petrochemical wastewater is applied to, is had a extensive future.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
SEQUENCE LISTING
<110>Tianjin Kai Ying sci-tech development Co., Ltd.s
<120>One plant of orthoresol degradation bacteria and its application
<130> PB610105N
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1408
<212> DNA
<213> Pseudomonas sp.
<400> 1
catgcaagtc gagcggatga cgggagcttg ctccttgatt cagcggcgga cgggtgagta 60
atgcctagga atctgcctgg tagtggggga caacgtttcg aaaggaacgc taataccgca 120
tacgtcctac gggagaaagc aggggacctt cgggccttgc gctatcagat gagcctaggt 180
cggattagct agttggtggg gtaatggctc accaaggcga cgatccgtaa ctggtctgag 240
aggatgatca gtcacactgg aactgagaca cggtccagac tcctacggga ggcagcagtg 300
gggaatattg gacaatgggc gaaagcctga tccagccatg ccgcgtgtgt gaagaaggtc 360
ttcggattgt aaagcacttt aagttgggag gaagggcagt aagttaatac cttgctgttt 420
tgacgttacc gacagaataa gcaccggcta actctgtgcc agcagccgcg gtaatacaga 480
gggtgcaagc gttaatcgga attactgggc gtaaagcgcg cgtaggtggt ttgttaagtt 540
ggatgtgaaa gccccgggct caacctggga actgcatcca aaactggcaa gctagagtac 600
ggtagagggt ggtggaattt cctgtgtagc ggtgaaatgc gtagatatag gaaggaacac 660
cagtggcgaa ggcgaccacc tggactgata ctgacactga ggtgcgaaag cgtggggagc 720
aaacaggatt agataccctg gtagtccacg ccgtaaacga tgtcaactag ccgttggaat 780
ccttgagatt ttagtggcgc agctaacgca ttaagttgac cgcctgggga gtacggccgc 840
aaggttaaaa ctcaaatgaa ttgacggggg cccgcacaag cggtggagca tgtggtttaa 900
ttcgaagcaa cgcgaagaac cttaccaggc cttgacatgc agagaacttt ccagagatgg 960
attggtgcct tcgggaactc tgacacaggt gctgcatggc tgtcgtcagc tcgtgtcgtg 1020
agatgttggg ttaagtcccg taacgagcgc aacccttgtc cttagttacc agcacgttat 1080
ggtgggcact ctaaggagac tgccggtgac aaaccggagg aaggtgggga tgacgtcaag 1140
tcatcatggc ccttacggcc tgggctacac acgtgctaca atggtcggta cagagggttg 1200
ccaagccgcg aggtggagct aatctcacaa aaccgatcgt agtccggatc gcagtctgca 1260
actcgactgc gtgaagtcgg aatcgctagt aatcgcgaat cagaatgtcg cggtgaatac 1320
gttcccgggc cttgtacaca ccgcccgtca caccatggga gtgggttgca ccagaagtag 1380
ctagtctaac cttcgggagg acggtacc 1408
Claims (10)
1. one plant of orthoresol degradation bacteria (Pseudomonas sp.) TJKYLJF-01, its deposit number is CGMCC NO.10177.
2. orthoresol degradation bacteria TJKYLJF-01 as claimed in claim 1, it is characterised in that its condition of culture is to be connect
Plant in culture medium, temperature is 25-35 DEG C, and initial pH is 7-9, and inoculum concentration is 1-3%, and shaking speed is 100-150rpm.
3. orthoresol degradation bacteria TJKYLJF-01 as claimed in claim 2, it is characterised in that the formula of the culture medium is,
K2HPO4·12H2O 2.24g, KH2PO42.74g, (NH4)2SO41g, MgCl2·6H2O 0.2g, NaCl 0.1g, FeCl3·
6H2O 0.02g, CaCl20.01g, 2mL trace element storing solution, supply distilled water to 1000mL；The micro- storing solution
Component is MnSO4·H2O 0.1g, ZnSO4·7H2O 0.12g, H3BO30.07g, Na2MoO4·H2O 0.04g, CuSO4·
5H2O 0.02g, CoCl20.04g, supplies distilled water to 1000mL.
4. the orthoresol degradation bacteria TJKYLJF-01 as described in claim 1-3 is any, it is characterised in that be resistant to concentration≤
The orthoresol of 1500mg/L.
5. the microbial inoculum of any orthoresol degradation bacteria TJKYLJF-01 of claim 1-4 is contained.
6. described in any described orthoresol degradation bacteria TJKYLJF-01 of claim 1-4 or its zymotic fluid or claim 5
Application of the microbial inoculum in orthoresol of degrading.
7. application as claimed in claim 6, it is characterised in that concentration≤1500mg/L of the control orthoresol in system；
The condition of work of degradation process is：15-40 DEG C of temperature, preferably 25-35 DEG C；PH value 5-9, preferable ph 7-8.
8. described in any described orthoresol degradation bacteria TJKYLJF-01 of claim 1-4 or its zymotic fluid or claim 5
Application of the microbial inoculum in purified industrial waste water.
9. application as claimed in claim 8, it is characterised in that described industrial wastewater be coking chemical waste water, coal chemical industrial waste water and
One kind in petrochemical wastewater.
10. the microbial inoculum described in the orthoresol degradation bacteria TJKYLJF-01 or its zymotic fluid or claim 5 described in claim 1 exists
Application in clean environment.
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