CN101240256A - Citrobacter farmeri Citrobacter farmeriSC01 and application thereof - Google Patents
Citrobacter farmeri Citrobacter farmeriSC01 and application thereof Download PDFInfo
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Abstract
The invention discloses a fashi citric acid rod Citrobacter farmeri SC01 and the application. The bacterium is obtained by artificial accumulation culture and separation cleansing from phenols cyanogen waste water treatment station one level aerobic pool sludge. The bacterium is preserved in china typical culture entreasure center of Wuhan University on January 7th 2008 year whose short form is CCTCC and preserving identification serial number is CCTCC NO: M208004. The fashi citric acid rod Citrobacter farmeri SC01 incorporating the invention can degrade phenyl hydrate, 2-methylphenol, m-cresol, 1-naphthyl hydroxide, 696mg/L m-cresol in 114 hours on aerobic condition, and the average degrading rate of speed ran up to 8.94mg/(L h) and the bacterium is sensitive to acheomycin, chloramphenicol, rifampicin and streptomycin, and the bacterium can be used safe and wide in harnessing and repairing wasted water and soil phenols pollution because the bacterium can not carryover drug-resistance factor spreading in aborigines bacterium when the bacterium is released into environment.
Description
Technical field
The present invention relates to a kind of Fa Shi citric acid bacillus Citrobacter farmeri SC01 and application thereof that can the efficient degradation phenols.
Background technology
Phenolic compound is made of a series of phenol and derivative thereof, boiling point＜230 ℃ be called volatile phenol.Phenol has a strong impact on surface water quality after entering water body, makes aquatic original biological death.Tap water is phenol-polluted, smells and distinguish the flavor of all can change.The manufacturing of phenol, coking, oil refining, metallurgy, plastics, chemical fibre, insulating material, resol, pharmacy, explosive, agricultural chemicals or the like industry all can have the phenolic wastewater of higher concentration.
Phenolic compound is a kind of prototype matter poisonous substance, and all life individualities are all had toxic action, can make protein coagulating, so the intensive germicidal action is arranged.Aldehydes matter all can produce murder by poisoning to living organisms, and the water that people's long-term drinking is polluted by phenol can cause giddy, anaemia and various nervous system disorders; Phenols content is greater than 10mg/L in water, and hydrobionts such as fish can not survive; Contain the high waste water of phenol concentration if be used for irrigating, will cause the underproduction of farm crop and withered.Phenolic wastewater is to having a strong impact on of causing of people's life and endanger, and is classified as one of harmful waste water that emphasis solves by China.
The common method of Phenol-Containing Wastewater Treatment mainly contains physics method (absorption method, extraction process etc.), chemical method (chemical oxidation, photochemical catalytic oxidation etc.) and biological process (activated sludge process, biomembrance process etc.) both at home and abroad at present.The treatment process different according to the different choice of waste strength.To contain phenol concentration higher (＞2000mg/L), waste water that toxicity is stronger, generally adopt physics method and chemical method; When the phenolic compound concentration in the waste water lower (＜2000mg/L) time, be suitable for handling with biological process.Biological process is compared with chemical method with the physics method, has economy, efficient, innoxious, non-secondary pollution and the big advantage of treatment capacity.
Atagana, Harrison etc. filter out 7 kinds of non-load class fungies and 2 kinds of load class fungies from oil pollution soil, with their degradation of phenol, meta-cresol, ortho-cresol and p-cresol mixture.Pseudomonas CF600, feathering testosterone Zymomonas mobilis ZD4-1, rhodococcus PNAN5, bacillus cereus Jp-A, Bacillus Cro3.2, Pseudomonas vesicularis, Alcaligenes eutrophus, Pseudomonas putida EKII, Acinetobacter calcoaceticus NCIB8250 and Pseudomonas aeruginosa ZD4-3 etc. also have report to the degraded of phenol, and the aerobic microbiological of the cresols of degrading mainly contains yeast, xanthin bacterium and white-rot fungi.
Because the meta-cresol of high density can suppress microbial growth, makes the overstand of wastewater treatment, processing efficiency is lower.It is generally acknowledged that the traditional biological facture is only applicable to phenolic compound concentration below 500mg/L.The existing bacterial classification that can degrade meta-cresol mainly contains candida tropicalis bacterium, maltose candidiasis, variegated rainbow conk and some anaerobism and produces alkane microorganism etc.People such as Fialova.Arianna research is weighed phenol compound by the biodegradable fast monitored of maltose candidiasis with BOD, discover, when phenol concentration is 1.7g/L, when benzene phosphorus diphenol concentration is 1.5g/L, yeast can utilize them as the unique carbon source and the energy, even if Resorcinol concentration this moment reaches 2g/L, can their degraded not exerted an influence yet, p-cresol also can be degraded simultaneously; Wang Lunji utilizes variegated rainbow conk that cresols is degraded, discover that (100mg/L) descends variegated rainbow conk that the average degradation rate of cresols is reached 74.8% under the lower concentration, (rising with its concentration reduces by 500～1500mg/L) variegated rainbow conk to the degradation rate of cresols, and minimum is 12.13% under high density.Variegated rainbow conk is the trend that increases considerably with the concentration rising earlier to the average absolute degradation amount of cresols degraded, is up to 265.1mg/L; The Fang of Hong Kong University, Herbert H.P. is by pointing out that to the research of producing alkane microbiological deterioration methylphenol Ortho Cresol and m-cresol (when concentration is 225mg/L separately) can partly be degraded; And can degrade fully in the 52h meta-cresol of 280mg/L of candida tropicalis bacterium is degradation process the most efficiently, by the Jiang Yan of University Of Tianjin, hear the Jianping and reported.Yet industries such as coking, petrochemical industry, oil gas, chemical fibre, pharmacy are discharged and are contained the concentration of polyphenol waste water far above this.
Summary of the invention
The objective of the invention is problem at the prior art existence, a kind of Fa Shi citric acid bacillus Citrobacter farmeri SC01 of energy efficient degradation phenols is provided, this bacterium can be at efficient degradation meta-cresol under the aerobic condition, and can utilize multiple phenols such as phenol, ortho-cresol, 1-naphthols.
Another object of the present invention provides the application of above-mentioned Fa Shi citric acid bacillus Citrobacterfarmeri SC01 in the multiple phenol organic matter of degraded.
Fa Shi citric acid bacillus Citrobacter farmeri SC01 of the present invention is preserved in Wuhan University China typical culture collection center on January 7th, 2008, and it abbreviates CCTCC as, and deposit number is CCTCC NO:M208004.
One, the separation and purification of efficient degradation phenols bacterial strain
Get Guangdong splendid steel Coking Plant Wastewater treatment station one-level Aerobic Pond mud, mud is placed triangular flask, do not add the nutrition aeration 48 hours, standing sedimentation, filtration are got supernatant liquor access phenols degradation bacteria and are selected substratum (to select culture medium prescription to be: K
2HPO
42.24g/L, KH
2PO
42.75g/L, (NH
4)
2SO
41g/L, MgCl
26H
2O 0.2g/L, NaCl 0.1mg/L, FeCl
36H
2O 0.02g/L, CaCl
20.01mg/L, the pH value of regulating substratum is 6.8～7.0, sterilized 30 minutes down for 121 ℃), and add phenol 100mg/L and meta-cresol 20mg/L is a carbon source, in the constant temperature shaking table 30 ℃, 130 rev/mins of shaking culture, the failure of oscillations is cultivated after treating the solution muddiness, nutrient solution is inserted fresh selection substratum again, phenol concentration wherein suitably improves, and through repeatedly domestication cultivation, reaches 500mg/L until phenol concentration like this, meta-cresol concentration reaches 50mg/L, and the nutrient solution that obtains both had been phenols degradation bacteria suspension (as phenols degradation bacteria source).
(the LB culture medium prescription is: extractum carnis 5g/L, peptone 10g/L, NaCl 5g/L to change above-mentioned bacteria suspension over to the LB plate culture medium, the pH value of regulating substratum is 7.0～7.2, the agar of adding 1.75% when system is dull and stereotyped), add 1 milliliter of 50mg/L meta-cresol again, evenly be coated with back method of scoring isolate suspension with glass rod, cultivated 24 hours in 30 ℃ constant incubators, obtain single bacterium colony.Because each single bacterium colony is all represented pure bacterium, therefore the bacterium that can grow on this substratum all has the function of degraded meta-cresol, obtains pure culture meta-cresol efficient degrading bacteria SC01 thus.
Two, the character of bacterial strain SC01
The pure culture meta-cresol efficient degrading bacteria SC01 that separation and purification is obtained carries out various character evaluations, and the result is as follows:
1, morphological specificity
The bacterium colony of this bacterium is cream-colored, and smooth surface is opaque, neat in edge, and the thalline quarter butt is to oval.
2, cultural characters
The optimum growing condition of this bacterium is: pH6～7,30～35 ℃.
3, physiological property
This bacterium is the Gram-negative bacterium, catalase feminine gender, fermented type, amphimicrobian.
This bacterium is very responsive to tsiklomitsin, and paraxin, Rifampin, penicillin medium sensitivity are to the Streptomycin sulphate muting sensitive.
4, functional performance
This bacterium can utilize meta-cresol as sole carbon source and energy growth and breeding, and under the pure culture condition, can degrade the fully meta-cresol of 696mg/L of this bacterium also can utilize phenol, ortho-cresol, 1-naphthols to be sole carbon source and energy growth and breeding respectively, simultaneously with its degraded.
According to morphologic observation and physiological and biochemical test, and consult " Bergey ' s Manual ofDeterminative Bacteriology " and " common bacteria system identification handbook ", find that bacterial strain SC01 is consistent with Fa Shi citric acid bacillus (Citrobacter farmeri) most.
Three, the 16S rDNA sequencing of bacterial strain SC01
The above-mentioned pure culture meta-cresol of picking efficient degrading bacteria SC01 inserts in about 20 μ L aseptic double-distilled waters, and as pcr template, amplification 16S rDNA to 1.5kb checks order and identifies that the result is shown in SEQ ID NO:1 boiling water bath after 5 minutes.
The base sequence that order-checking is obtained carries out homologous sequence search (blast search) by the Internet in international nucleic acid sequence data storehouses such as GenBank, find out the type strain that homology is the highest in this strain bacterium and the database or be preserved in ATCC or the bacterial strain of international DSMZ such as DSM, found that bacterial classification SC01 and Fa Shi citric acid bacillus Citrobacter farmeri have highest homology and reach 99%.
Comprehensively, can determine that the pure culture meta-cresol efficient degrading bacteria that separation and purification of the present invention obtains is Fa Shi citric acid bacillus Citrobacter farmeri SC01 to morphologic observation, Physiology and biochemistry result and the 16S rDNA molecule sequencing result of bacterial strain SC01.
Compared with prior art, the present invention has following beneficial effect: 1. Fa Shi citric acid bacillus Citrobacter farmeri SC01 of the present invention can be under aerobic condition degradation of phenol, ortho-cresol, meta-cresol, 1-naphthols fully, meta-cresol through 114 hours 696mg/L that degrade fully, its average degradation rate reaches as high as 8.94mg/ (Lh), far above present bibliographical information level; 2. Fa Shi citric acid bacillus Citrobacter farmeri SC01 of the present invention is to tsiklomitsin, paraxin, Rifampin and Streptomycin sulphate sensitivity, therefore when this thalline is released in the physical environment, can not carry resistance determining factor simultaneously and between indigenous bacterium, propagate, thereby make this bacterium obtain safety in can and repairing in improvement that waste water, soil phenols pollute, use widely.
Description of drawings
Fig. 1 is the pcr amplification program of the 16SrDNA of Fa Shi citric acid bacillus Citrobacter farmeri SC01;
Fig. 2 is the growth of Fa Shi citric acid bacillus Citrobacter farmeri SC01 and the degradation curve figure of meta-cresol;
Fig. 3 is the UV collection of illustrative plates of Fa Shi citric acid bacillus Citrobacter farmeri SC01 degraded meta-cresol process;
Fig. 4 is the Fa Shi citric acid bacillus Citrobacter farmeri SC01 different starting point concentration meta-cresol graphic representations of degrading;
Fig. 5 is the degrade graphic representation of different substrates of Fa Shi citric acid bacillus Citrobacter farmeri SC01.
Embodiment
The separation and purification of embodiment 1 efficient degradation phenols bacterial strain
Get Guangdong splendid steel Coking Plant Wastewater treatment station one-level Aerobic Pond mud, mud is placed triangular flask, do not add the nutrition aeration 48 hours, standing sedimentation, filtration are got supernatant liquor access phenols degradation bacteria and are selected substratum (to select culture medium prescription to be: K
2HPO
42.24g/L, KH
2PO
42.75g/L, (NH
4)
2SO
41g/L, MgCl
26H
2O 0.2g/L, NaCl 0.1mg/L, FeCl
36H
2O 0.02g/L, CaCl
20.01mg/L, the pH value of regulating substratum is 6.8～7.0, sterilized 30 minutes down for 121 ℃), and add phenol 100mg/L and meta-cresol 20mg/L is a carbon source, in the constant temperature shaking table 30 ℃, 130 rev/mins of concussions are cultivated, treat to stop the concussion cultivation after the solution muddiness, nutrient solution is inserted fresh selection substratum again, phenol concentration wherein suitably improves, and through repeatedly domestication cultivation, reaches 500mg/L until phenol concentration like this, meta-cresol concentration reaches 50mg/L, and the nutrient solution that obtains both had been phenols degradation bacteria suspension (as phenols degradation bacteria source).
(the LB culture medium prescription is: extractum carnis 5g/L, peptone 10g/L, NaCl 5g/L to change above-mentioned bacteria suspension over to the LB plate culture medium, the pH value of regulating substratum is 7.0～7.2, the agar of adding 1.75% when system is dull and stereotyped), add 1 milliliter of 50mg/L meta-cresol again, evenly be coated with back method of scoring isolate suspension with glass rod, cultivated 24 hours in 30 ℃ constant incubators, obtain single bacterium colony.Because each single bacterium colony is all represented pure bacterium, therefore the bacterium that can grow on this substratum all has the function of degraded meta-cresol, obtains pure culture meta-cresol efficient degrading bacteria SC01 thus.
The character of embodiment 2 bacterial strain SC01
The pure culture meta-cresol efficient degrading bacteria SC01 that 1 separation and purification obtains to embodiment carries out various character and identifies that the result is as shown in table 1:
The morphologic observation of table 1 bacterial strain SC01 and Physiology and biochemistry experimental result
| Sequence number | Detect content | The |
| 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 | Morphologic observation gramstaining catalase oxydase anaerobic growth glucose oxidase fermentation V-P measures nitrate reduction starch gelatin hydrolysate liquefaction sugar, alcohols fermentation phenylalanine deaminase Citrate trianion utilizes lecithinase indole methyl red (MR) hydrogen sulfide lysine decarboxylase ONPG to measure | Cream-colored, smooth surface is opaque, the neat in edge Gram-negative, quarter butt to oval+-+, facultative anaerobe+, fermented type-+--galactitol-, (N.F,USP MANNITOL, sorbyl alcohol, raffinose, lactose, sucrose, trehalose, wood sugar, rhamnosyl, pectinose, melibiose)+-+-++--+ |
Annotate :+expression positive reaction ,-expression negative reaction.
According to morphologic observation and physiological and biochemical test, and consult " Bergey ' s Manual ofDeterminative Bacteriology " and " common bacteria system identification handbook ", find that bacterial classification SC01 is consistent with Fa Shi citric acid bacillus (Citrobacter farmeri) most.
The 16S rDNA sequencing of embodiment 3 bacterial strain SC01
The above-mentioned pure culture meta-cresol of picking efficient degrading bacteria SC01 inserts in about 20 μ L aseptic double-distilled waters, boiling water bath after 5 minutes as pcr template, amplification 16S rDNA to 1.5kb, the amplification reaction system of PCR is 50 μ L:
Aseptic double-distilled water 36.5 μ L
10 * PCR damping fluid, 5.0 μ L
2.5mmol·L
-1dNTP 5.0μL
10μmol·L
-1F27 1.0μL
10μmol·L
-1R1522 1.0μL
5U·(μL)
-1Blend-Taq 0.5μL
Template DNA 1.0 μ L
The PCR response procedures as shown in Figure 1.
The amplified production order-checking is identified that sequencing result is compared by GenBank shown in SEQ ID NO:1, bacterial classification SC01 and Fa Shi citric acid bacillus Citrobacter farmeri have highest homology and reach 99%.
The molecule sequencing result of morphologic observation, Physiology and biochemistry result and the embodiment 3 of comprehensive embodiment 2, the pure culture meta-cresol efficient degrading bacteria SC01 that the separation and purification of the present invention of can reaching a conclusion obtains is Fa Shi citric acid bacillus Citrobacter farmeri SC01.
The performance of embodiment 4 Fa Shi citric acid bacillus Citrobacter farmeri SC01 degraded meta-cresol
Fa Shi citric acid bacillus Citrobacter farmeri SC01 is seeded in the selection substratum that contains the about 40mg/L of meta-cresol, shaking culture in 170 rev/mins, 30 ℃ shaking tables, per hour took a sample, took a sample at 14,19,24 hours afterwards in preceding 10 hours, measure the meta-cresol residual concentration in the nutrient solution respectively, the results are shown in Figure 2.In the meta-cresol degradation curve of Fig. 2, Citrobacter farmeri SC01 does not have the lag phase substantially to the degraded of meta-cresol, degradation speed reaches maximum after the 4th hour, degrade fully at the 9th hour meta-cresol, cell concentration increases by 1.5 times during this period, and owing to the deficiency of carbon source (meta-cresol), the further propagation of thalline lacks the carbon source support afterwards, growth begins to enter endogenous stage of exhaustion, and biomass begins to descend.
Along with the domestication process of continually strengthening, this bacterium is progressively improved the degradation capability of meta-cresol, after carried out degradation process again UV scanning see Fig. 3.Therefrom as can be seen, the characteristic absorbance peak maximum of meta-cresol is near 271 nanometers, and this wave band also is the charateristic avsorption band of phenyl ring.Along with the carrying out of degraded, the amount of meta-cresol (and intermediate product) constantly reduces, and at the 7th hour, characteristic peak disappeared, and shows that the intermediate product of meta-cresol and band phenyl ring thereof is all degraded fully.
The degrade performance of different starting point concentration meta-cresols of embodiment 5 Fa Shi citric acid bacillus Citrobacter farmeri SC01
Fa Shi citric acid bacillus Citrobacter farmeri SC01 is connected in the m-cresol solution of different starting point concentrations (concentration of each solution is as shown in table 2), investigates it to the ballistic tolerance of concentration, experimental result is seen Fig. 4.As can be seen from Figure 4, meta-cresol concentration is between 100～700mg/L the time, this bacterium all has the adaptive phase of certain hour to the degraded of meta-cresol, along with the raising of starting point concentration, the adaptive phase increases (seeing Table 2), under the situation of not deducting the adaptive phase, the trend that average degradation rate has raising to reduce again, when 304mg/L, reach maximum value, because the adaptive phase is long, cause average degradation rate to reduce when concentration is higher.Fa Shi citric acid bacillus Citrobacter farmeri SC01 is to the condition experiment of different concns meta-cresol degraded, show that its meta-cresol to high density has stronger tolerance and degraded to utilize ability, the ability that possesses anti-concentration impact load in the actual waste water treating processes has the applications well prospect.
Fa Shi citric acid bacillus Citrobacter farmeri in the different starting point concentration meta-cresols of table 2
The degradation rate of SC01
| Starting point concentration (mg/L) | 211 | 304 | 408 | 475 | 604 | 697 |
| Average degradation rate (mg/ (Lh) of adaptive phase (h) | 15 8.15 | 17 8.94 | 25 7.70 | 32 7.43 | 50 7.50 | 72 6.11 |
Embodiment 6 Fa Shi citric acid bacillus Citrobacter farmeri SC01 are to the degraded of multiple phenol
Fa Shi citric acid bacillus Citrobacter farmeri SC01 is connected in phenol, ortho-cresol, meta-cresol and the 1-naphthol reaction liquid of different concns, investigates its degradation capability to other phenols, the degradation process curve is seen Fig. 5.Degradation speed size between them is phenol＞p-cresol＞meta-cresol＞1-naphthols.Cause this result's reason to be, the substituting group on the phenyl ring is divided into an ortho-para directing group and bit-by-bit base two classes a :-O
-,-NH
2,-OH ,-C
6H
5,-CH
3,-X etc. has only singly-bound or electronegative, and they have adjacency pair position orientation effect, can make phenyl ring activation (except the halogen);-NO
2,-COOH etc. has Multiple Bonds or positively charged, position orientation effect between this class substituting group has, and substitution reaction promptly can make the phenyl ring passivation all than benzene difficulty.Phenolic hydroxyl group on the phenyl ring can activate the ortho para group, thereby when methyl is in ortho para than at the easier quilt in a position, attack, therefore ortho para cresols is degraded than meta-cresol is easier, under the starting point concentration of about 42mg/L, the required degradation time of phenol, p-cresol and meta-cresol was respectively 11 hours, 12 hours and 13 hours; And the 1-naphthols has the relatively more stable structure of dicyclo, relative monocycle phenols, and degradation speed is the slowest, when starting point concentration is 22mg/L, needs ability degraded in 28 hours to finish.This example explanation Fa Shi citric acid bacillus Citrobacter farmeri SC01 dissimilar phenols of can degrading plays a role in the actual waste water of multiple phenol coexistence.
The antibiotics sensitivity experiment of embodiment 7 Fa Shi citric acid bacillus Citrobacter farmeri SC01
The Oxford agar diffusion method is adopted in antibiotics sensitivity experiment, several frequently seen microbiotic such as penicillin, paraxin, Streptomycin sulphate, tsiklomitsin and Rifampin is made into finite concentration respectively experimentizes.At first get 0.1mL 1 * 10
6The bacterium liquid of g/mL is applied to the 20mL substratum, each dull and stereotyped going up is placed 3 Oxford cups, in the cup of Oxford, add the 0.1mL microbiotic, spend the night in 32 ℃ of incubators, the susceptibility experiment is evaluated with antibacterial circle diameter: inhibition zone is defined as extremely quick more than 20nm, and 15-20mm is Gao Min, 10-14mm is quick in being, less than 10mm is muting sensitive, and what do not have inhibition zone is unwise, and Fa Shi citric acid bacillus Citrobacter farmeri SC01 sees Table 3 to above-mentioned 5 kinds of antibiotic susceptibility experimental results.This example explanation is rendered in the mud that physical environment or actual waste water handle through breadboard screening again when thalline, can not propagate between indigenous bacterium because of carrying resistance determining factor, has possessed the feasibility of practical application.
The antibiotics sensitivity of table 3 Fa Shi citric acid bacillus Citrobacter farmeri SC01 is real
Test the result
| Sequence number | The microbiotic title | Dosage (μ g) | Average diameter of inhibition zone (mm) | Result of |
| 1 2 3 4 5 | Penicillin streptomycin Rifampin paraxin tsiklomitsin | 10 10 5 30 30 | 0 9.3 10.3 12.0 20.3 | Unwise-muting sensitive+in quick ++ in quick ++ Gao Min ++ ++ |
A kind of Fa Shi citric acid bacillus Citrobacter farmeri SC01 and application sequence table .txt thereof
SEQUENCE LISTING
Claims (3)
1, a kind of Fa Shi citric acid bacillus SC01, the 16S rDNA sequence of this bacterium is preserved in Wuhan University China typical culture collection center on January 7th, 2008 shown in SEQ IDNO:1, and it abbreviates CCTCC as, and deposit number is CCTCC NO:M208004.
2, the application of the described Fa Shi citric acid bacillus of claim 1 SC01 in degrading phenol organic matter.
3, the application of Fa Shi citric acid bacillus SC01 according to claim 2 in degrading phenol organic matter is characterized in that described phenol organic matter is phenol, ortho-cresol, meta-cresol or 1-naphthols.
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| CN101531976B (en) * | 2009-04-21 | 2011-06-15 | 中山大学 | Citrobacter sp. strain DBM and method for treating acid mine drainage (AMD) using same |
| CN102220259A (en) * | 2011-04-21 | 2011-10-19 | 中国科学院过程工程研究所 | Sulfate reducing Citrobacter sp.strain HCSR and use thereof |
| CN102399701A (en) * | 2011-11-09 | 2012-04-04 | 武汉工程大学 | Phenol-degrading fungi and application thereof |
| CN102424804A (en) * | 2011-12-14 | 2012-04-25 | 青岛思普润水处理有限公司 | Citrobacter sp. for removing H2S gas from gas, and use thereof |
| CN102774963A (en) * | 2011-05-09 | 2012-11-14 | 北京化工大学 | Treatment method for waste water containing phenol |
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| CN101531976B (en) * | 2009-04-21 | 2011-06-15 | 中山大学 | Citrobacter sp. strain DBM and method for treating acid mine drainage (AMD) using same |
| CN102220259A (en) * | 2011-04-21 | 2011-10-19 | 中国科学院过程工程研究所 | Sulfate reducing Citrobacter sp.strain HCSR and use thereof |
| CN102774963A (en) * | 2011-05-09 | 2012-11-14 | 北京化工大学 | Treatment method for waste water containing phenol |
| CN102399701B (en) * | 2011-11-09 | 2013-07-10 | 武汉工程大学 | Phenol-degrading fungi and application thereof |
| CN102399701A (en) * | 2011-11-09 | 2012-04-04 | 武汉工程大学 | Phenol-degrading fungi and application thereof |
| CN102424804B (en) * | 2011-12-14 | 2014-07-09 | 青岛思普润水处理有限公司 | Citrobacter sp. for removing H2S gas from gas, and use thereof |
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| CN105255797A (en) * | 2015-11-30 | 2016-01-20 | 北京林业大学 | Free living nitrogen fixing bacteria MBC7 and application thereof |
| CN105399213A (en) * | 2015-12-12 | 2016-03-16 | 常州大学 | Microbial degradation method for phenol-containing waste water |
| CN106085447A (en) * | 2016-06-17 | 2016-11-09 | 战锡林 | Phenol contaminated soil remediation material |
| CN106754512A (en) * | 2016-12-22 | 2017-05-31 | 天津凯英科技发展股份有限公司 | One plant of orthoresol degradation bacteria and its application |
| CN107460141A (en) * | 2017-03-31 | 2017-12-12 | 佛山市玉凰生态环境科技有限公司 | A kind of heterotrophic nitrification aerobic denitrifying citric acid bacillus and its application |
| CN107460141B (en) * | 2017-03-31 | 2020-09-25 | 佛山市玉凰生态环境科技有限公司 | Heterotrophic nitrification aerobic denitrification citrobacter and application thereof |
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