CN105462901B - One pyridine degradation bacterium strain and its application - Google Patents
One pyridine degradation bacterium strain and its application Download PDFInfo
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Abstract
The invention discloses a pyridine degradation bacterium strain and its applications.Pyridine degradation bacterium strain TJKYBD-01 provided by the present invention belongs to Rhod (Rhodococcus sp.), and deposit number is CGMCC NO.10175.The bacterium is gram-positive bacteria, bacterium colony is pink, neat in edge, it is rounded, it is smooth wet, central protuberance, pyridine be can use as sole carbon source, nitrogen source and the energy and carry out growth and breeding, it is in 48h that the pyridine degradable of≤1000mg/L is complete, and it is resistant to the pyridine at concentrations up to 1500mg/L, there is excellent degradation effect to higher concentration pyridine industrial wastewater, which can be applied to the biological reinforced processing of coking wastewater or coal chemical industrial waste water or petrochemical wastewater, have a extensive future.
Description
Technical field
The present invention relates to microorganism and biodegradation technique fields, and in particular, to a pyridine degradation bacterium strain and its application,
Application especially in coking, coal chemical industry, petrochemical wastewater processing.
Background technique
Pyridine and its derivatives are typical polycyclic aromatic nitrogen-contg heterocyclic compounds, be widely present in Dippel's oil, coal tar,
Coal gas, shale oil, in petroleum.Pyridine has intense irritation, can anaesthetize central nervous system.To eye and the irritating work of the upper respiratory tract
With.After high concentration sucking, less serious case has glad or sensation of asphyxia, followed by appearance depression, myasthenia, vomiting, the severe one loss of consciousness, size
Fecal incontinence, tonic spasm, blood pressure decline.Pyridine is difficult to biodegrade, easily passes through coking, coal chemical industry, petrochemical wastewater
Outlet pollution of ecological environment has huge potential hazard to human health.
In wastewater treatment process, biological treatment has many advantages, if processing cost is lower, condition temperature than physico-chemical process
With do not generate secondary pollution etc..However, traditional biologic treating technique is extremely difficult to ring in some intractable industrial wastewaters
Guaranteed request, conventional activated sludge are at one's wit's end to the hard-degraded substance in waste water such as pyridine, quinoline, naphthalene etc..In recent years, biological
Reinforcement technique improves removal of original activated sludge to target contaminant by the way that microorganisms with specific functions is added in activated sludge
Efficiency substantially increases sewage treatment capacity, thus increasingly attracts people's attention.
Currently, existing some scholars filter out the bacterial strain for having efficient degradation performance to pyridine, such as pseudomonad both at home and abroad
(Pseudomonas sp.), thiobacilli (Thiobacillus), paracoccus (Paracoccus sp.) etc., these grind
Study carefully and only rests on laboratory stage mostly.Up to the present, there are some scholars both at home and abroad research shows that Rhod
(Rhodococcus sp.) can degrade sodium benzoate, catechol, resorcinol, hydroquinone and Polychlorinated biphenyls etc. is a variety of
Aromatic compound, but have no the report of its pyridine of degrading.Containing certain in coking wastewater, coal chemical industrial waste water and petrochemical wastewater
The pyridine of concentration, and pyridine belongs to bio-refractory organic matter, therefore only the ordinary activated sludge in biological treatment link is very
Hardly possible realizes effective degradation to pyridine.
Summary of the invention
It is an object of the invention to make up defect in the prior art, a pyridine degradation bacterium strain is provided, realizes it to coking
The biological reinforced processing of waste water, coal chemical industrial waste water and petrochemical wastewater.
Pyridine degradable bacteria TJKYBD-01 provided by the present invention is derived from the soil on Treatment of Coking Effluent station periphery.With pyridine
Growth and breeding is carried out as sole carbon source, nitrogen source and the energy, the flora in soil is carried out in pyridine minimal medium tame and docile
Change culture, and isolates and purifies to obtain by plate streaking.
Pyridine degradable bacteria provided by the present invention is gram-positive bacteria, and bacterium colony is pink, and neat in edge is rounded,
It is smooth wet, central protuberance.It is accredited as Rhod (Rhodococcus sp.) through 16S rRNA, names TJKYBD-01.It should
Bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on December 15th, 2014 (referred to as
CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101), it protects
Hiding number is CGMCC No.10175, entitled Rhodococcus sp of classifying (Rhodococcus sp.).
The condition of culture of pyridine degradable bacteria TJKYBD-01 provided by the present invention is to be inoculated in culture medium, temperature
It is 25-35 DEG C, initial pH is 7-9, and inoculum concentration 2%-5%, shaking speed is 100-150 rpm.
The formula of the culture medium is Na2HPO4·12H2O 4.26g, KH2PO42.65g MgSO4·7H2O 0.20g,
CaCl20.006g, FeSO4·7H2O 0.02g, 2mL microelement stock solution, supplies distilled water to 1000mL；The micro member
Plain stock solution group is divided into MnSO4·H2O 0.1g, ZnSO4·7H2O 0.12g, H3BO30.07g, Na2MoO4·H2O0.04g,
CuSO4·5H2O 0.02g, CoCl20.04g supplies distilled water to 1000mL.
Pyridine degradable bacteria TJKYBD-01 of the invention is resistant to the pyridine at concentrations up to 1500 mg/L.
Microbial inoculum containing pyridine degradable bacteria TJKYBD-01 of the present invention belongs to the scope of protection of the present invention.
Application the present invention provides pyridine degradable bacteria TJKYBD-01 or containing its microbial inoculum in purified industrial waste water.
The industrial wastewater is one of coking wastewater, coal chemical industrial waste water and petrochemical wastewater.
Pyridine degradable bacteria TJKYBD-01 of the invention can be grown using pyridine as sole carbon source, nitrogen source and the energy
Breeding, it is in 48 h that the pyridine degradable of 1000 mg/L is complete.
The pyridine degradable bacteria TJKYBD-01 of invention as sole carbon source, nitrogen source and the energy can grow numerous using pyridine
It grows, it is in 48h that the pyridine degradable of≤1000mg/L is complete.Pyridine degradable bacteria TJKYBD-01 is resistant at concentrations up to 1500
The pyridine of mg/L has excellent degradation effect to higher concentration pyridine industrial wastewater, which can be applied to coking wastewater, coalification
The biological reinforced processing of work waste water and petrochemical wastewater, has a extensive future.
Detailed description of the invention
Growth and the pyridine degradable effect relation figure that Fig. 1 is pyridine degradable bacteria TJKYBD-01.
Fig. 2 is influence diagram of the pyridine initial concentration to pyridine degradable bacteria TJKYBD-01 degradation pyridine.
Fig. 3 is influence of the cultivation temperature to pyridine degradable bacteria TJKYBD-01 degradation pyridine.
Fig. 4 is influence diagram of the initial pH value to pyridine degradable bacteria TJKYBD-01 degradation pyridine.
Fig. 5 is influence diagram of the inoculum concentration to pyridine degradable bacteria TJKYBD-01 degradation pyridine.
Fig. 6 is influence diagram of the shaking speed to pyridine degradable bacteria TJKYBD-01 degradation pyridine.
Fig. 7 is intensive treatment effect picture of the pyridine degradable bacteria TJKYBD-01 to the coking wastewater of addition higher concentration pyridine.
Fig. 8 is intensive treatment effect of the pyridine degradable bacteria TJKYBD-01 to the coal chemical industrial waste water of addition higher concentration pyridine
Figure.
Fig. 9 is that pyridine degradable bacteria TJKYBD-01 imitates the intensive treatment of the petrochemical wastewater of addition higher concentration pyridine
Fruit figure.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Without departing substantially from spirit of that invention
In the case where essence, to modifications or substitutions made by the method for the present invention, step or condition, all belong to the scope of the present invention.
Unless otherwise specified, chemical reagent used in embodiment is conventional commercial reagent, skill used in embodiment
The conventional means that art means are well known to those skilled in the art.
Separation, identification and its degradation property to pyridine of 1 pyridine degradable bacteria TJKYBD-01 of embodiment
1, the separation, purifying of bacterial strain
(1) strain source
Bacterium source picks up from the soil on Treatment of Coking Effluent station periphery, is in dark brown, acquires the pedotheque 6 away from surface layer 4-8cm
Part.
(2) bacterial strain isolates and purifies
Sterile distilled water sky is added in the soil sample of acquisition and exposes 48h, takes 10mL supernatant to be inoculated in 90mL after standing and contains
In the LB culture medium (peptone 1%, sodium chloride 1%, yeast extract 0.5%) of 300mg/L pyridine, in 30 DEG C and 150 r/
Min activation culture is for 24 hours.5mL culture solution is taken to be forwarded in the minimal medium of the 300mg/L containing pyridine, similarity condition domestication training
2-3d is supported, then takes 5mL culture solution transposing to fresh pyridine minimal medium, 3 times repeatedly, wherein pyridine concentration is distinguished
For 300,500,1000mg/L；The domestication liquid of the 1000mg/L containing pyridine is diluted by different proportion, is respectively coated on containing pyridine
On the pyridine inorganic salts plate of 500mg/L, 30 DEG C of culture 3d.Sharp-edged bacterium colony is selected, is purified to and obtains through LB plate streaking
Single colonie is obtained, carries out pyridine degradable test respectively.It is oblique with the LB of the 500mg/L containing pyridine after determining each strains for degrading ability for pyridine
The excellent bacterial strain of face preservation degradation property.
2, the identification of bacterial strain
(1) colony morphology characteristic and physio-biochemical characteristics of pyridine degradable bacteria TJKYBD-01
Pyridine degradable bacteria TJKYBD-01 bacterium colony is pink, and neat in edge is rounded, smooth wet, central protuberance.
(2) the 16S rRNA identification of pyridine degradable bacteria TJKYBD-01
The 16S rRNA gene of pyridine degradable bacteria TJKYBD-01 is cloned, is sequenced, is then carried out in GenBank
Blast is compared.The results show that reaching 99% with its sequence similarity is Rhod (Rhodococcus sp.),
16S rDNA sequence is as shown in sequence table.In conjunction with the physio-biochemical characteristics of bacterial strain, Rhod is belonged to
(Rhodococcus sp.), number TJKYBD-01.
The bacterial strain was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on December 15th, 2014
The heart (abbreviation CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode
100101), deposit number is CGMCC No.10175, entitled Rhodococcus sp of classifying (Rhodococcus sp.).
3, the preparation of bacteria suspension
One ring microbionation of picking in LB culture medium, 30 DEG C, 150rpm culture for 24 hours, take 5mL culture to be connected to fresh LB training
Base is supported, 30 DEG C of culture 7-8h (bacterium is in logarithmic growth phase), thalline were collected by centrifugation by 5000rpm, 10min, and inorganic with pyridine
Salt culture medium (pH7.5) washs 3 times, is resuspended in pyridine minimal medium, adjusts cell density to OD600=1,4 DEG C of preservations
It is spare.
4, to the growth of pyridine degradable bacteria TJKYBD-01 and its relationship to the degradation effect of pyridine
Pyridine degradable bacteria TJKYBD-01 is inoculated in the minimal medium of the pyridine containing 500mg/L, in 30 DEG C, 150r/
Min shaking table culture, the growth of METHOD FOR CONTINUOUS DETERMINATION cell and the residual concentration of pyridine are as shown in Figure 1, measurement result shows: pyridine degradable
The growth tendency of bacterium TJKYBD-01 and degradation process basic synchronization to pyridine, the bacterium can using pyridine as sole carbon, nitrogen and
Energy substance promotes own growth, and has good degradation effect to pyridine.
5, influence of the condition of culture to the growth of pyridine degradable bacteria TJKYBD-01 and its ability of degradation pyridine
(1) influence of pyridine initial concentration: investigate different pyridine initial concentrations (100,300,500,800,1000,
1500mg/L) to the influence of pyridine degradable bacteria TJKYBD-01 degradation pyridine, in METHOD FOR CONTINUOUS DETERMINATION culture solution, the residual concentration of pyridine is such as
Shown in Fig. 2, pyridine degradable bacteria TJKYBD-01 can be complete by the pyridine degradable of 1000mg/L in 48h, with pyridine initial concentration
Raising, deadtime needed for degradation process is obviously prolonged, when pyridine concentration reaches 1500mg/L, pyridine degradable bacteria
TJKYBD-01 becomes very slow to the removal of pyridine.The result shows that pyridine is to pyridine degradable bacteria with the raising of pyridine concentration
The Inhibition of degradation effect of TJKYBD-01 gradually increases.
(2) influence of cultivation temperature: different cultivation temperature (15,20,25,30,35,40 DEG C) is investigated to pyridine degradable bacteria
TJKYBD-01 degrades the influence of pyridine, and the residual concentration of pyridine is as shown in figure 3, pass through 48h at 30 DEG C in METHOD FOR CONTINUOUS DETERMINATION culture solution
Culture, about 1000mg/L pyridine only remnants 5.3mg/L, hence it is evident that better than the removal effect of other temperature.The experimental results showed that pyridine
The temperature comparisons of degradation bacteria TJKYBD-01 degradation pyridine are 30 DEG C of 25 DEG C of >, 35 DEG C of >, 40 DEG C of >, 15 DEG C of > of 20 DEG C of >.
(3) influence of initial pH: different initial pH value (3,4,5,6,7,8,9,10) is investigated to pyridine degradable bacteria
TJKYBD-01 degrades the influence of pyridine, and the residual concentration of pyridine is as shown in figure 4, according to degradation process in METHOD FOR CONTINUOUS DETERMINATION culture solution
The size of middle pyridine residual concentration determines pyridine degradable effect under different initial pH values: pH8 ≈ pH7 > pH9 > pH6 > pH5 >
PH4 > pH3.
(4) influence of inoculum concentration: investigate different inoculum concentrations (0.5vol%, 2vol%, 5vol%, 10vol%,
15vol%, 20vol%) influence to pyridine degradable bacteria TJKYBD-01 degradation pyridine, pyridine is residual in METHOD FOR CONTINUOUS DETERMINATION culture solution
Stay concentration as shown in Figure 5, it can be seen that inoculum concentration is bigger, and pyridine degradable rate is faster, when inoculum concentration be 10vol% and
When 15vol%, the removal rate highest of pyridine in shaking flask shows that simple rely on increases again at this time when inoculum concentration reaches 20vol%
Inoculum concentration is not obvious the influence for improving pyridine degradable effect.
(5) influence of shaking speed: different shaking speed (50,100,150,200,250rpm) is investigated to pyridine degradable
Bacterium TJKYBD-01 degrades the influence of pyridine, and the residual concentration of pyridine is as shown in fig. 6, revolving speed is 50r/ in METHOD FOR CONTINUOUS DETERMINATION culture solution
The degradation rate that the pyridine degradable rate of min is 100,150,200 and 250r/min significantly lower than revolving speed.In addition, experimental result is shown
Influence of the latter three to pyridine degradable rate be not fairly obvious, shows to be able to satisfy pyrrole when shaking speed is 100-150r/min
Demand of the pyridine degradation bacteria TJKYBD-01 to dissolved oxygen is simple at this time to rely on the influence to increase speed to raising pyridine degradable effect simultaneously
It is not significant.
Intensive treatment of the 2 pyridine degradable bacteria TJKYBD-01 of embodiment to the coking wastewater of addition higher concentration pyridine
In conical flask, the isolated pyridine degradable bacteria TJKYBD-01 of embodiment 1 is added to processing coking wastewater
In activated sludge, its removal effect to pyridine is investigated in 3 groups of experiments of setting.
(a) (formula of culture medium is Na to 32mL coking wastewater+33mL pyridine minimal medium2HPO4·12H2O
4.26g KH2PO42.65g MgSO4·7H2O 0.20g, CaCl20.006g, FeSO4·7H2O 0.02g, 2mL microelement
Stock solution supplies distilled water to 1000mL；The microelement stock solution group is divided into MnSO4·H2O 0.1g, ZnSO4·7H2O
0.12g, H3BO30.07g, Na2MoO4·H2O 0.04g, CuSO4·5H2O 0.02g, CoCl20.04g supplies distilled water extremely
1000mL.)+1000mg/L pyridine+30mL activated sludge+5vol% pyridine degradable bacteria TJKYBD-01 bacteria suspension；
(b) work after 32mL coking wastewater+33mL pyridine minimal medium+1000mg/L pyridine+30mL high pressure sterilization
The pyridine degradable bacteria TJKYBD-01 bacteria suspension of property sludge+5vol%；
(c) 32mL coking wastewater+38m pyridine minimal medium+1000mg/L pyridine+30mL activated sludge.In 30 DEG C,
150rpm shaking table culture.
The residual concentration of pyridine is as shown in Figure 7 in METHOD FOR CONTINUOUS DETERMINATION culture solution, the results showed that: pyridine degradable bacteria is not added
The activated sludge of TJKYBD-01 is slow to the pyridine degradable in waste water, adds pyridine degradable bacteria TJKYBD-01 and then significantly improves
Activated sludge is to the degradation capability of pyridine, and the pyridine that will contain about in 48h in the coking wastewater of 1000mg/L pyridine is gone completely
It removes.
Intensive treatment of the 3 pyridine degradable bacteria TJKYBD-01 of embodiment to the coal chemical industrial waste water of addition higher concentration pyridine
In conical flask, pyridine degradable bacteria TJKYBD-01 is added in the activated sludge of processing coal chemical industrial waste water, setting
Its removal effect to pyridine is investigated in 3 groups of experiments.
(a) 32mL coal chemical industrial waste water+33mL pyridine minimal medium+1000mg/L pyridine+30mL activated sludge+
The pyridine degradable bacteria TJKYBD-01 bacteria suspension of 5vol%；
(b) after 32mL coal chemical industrial waste water+33mL pyridine minimal medium+1000mg/L pyridine+30mL high pressure sterilization
The pyridine degradable bacteria TJKYBD-01 bacteria suspension of activated sludge+5vol%；
(c) 32mL coal chemical industrial waste water+38m pyridine minimal medium+1000mg/L pyridine+30mL activated sludge.In 30
DEG C, 150rpm shaking table culture.
The residual concentration of pyridine is as shown in Figure 8 in METHOD FOR CONTINUOUS DETERMINATION culture solution, the results showed that: pyridine degradable bacteria TJKYBD-01
Apparent bioaugmentation is played, and the pyridine that will contain about in 48h in the coal chemical industrial waste water of 1000mg/L pyridine is complete
Removal.
Intensive treatment of the 4 pyridine degradable bacteria TJKYBD-01 of embodiment to the petrochemical wastewater of addition higher concentration pyridine
In conical flask, pyridine degradable bacteria TJKYBD-01 is added in the activated sludge of processing petrochemical wastewater, if
Set the removal effect that 3 groups of experiments investigate it to pyridine.
(a) 32mL petrochemical wastewater+33mL pyridine minimal medium+1000mg/L pyridine+30mL activated sludge+
The pyridine degradable bacteria TJKYBD-01 bacteria suspension of 5vol%；
(b) after 32mL petrochemical wastewater+33mL pyridine minimal medium+1000mg/L pyridine+30mL high pressure sterilization
Activated sludge+5vol% pyridine degradable bacteria TJKYBD-01 bacteria suspension；
(c) 32mL petrochemical wastewater+38m pyridine minimal medium+1000mg/L pyridine+30mL activated sludge.In
30 DEG C, 150rpm shaking table culture.
The residual concentration of pyridine is as shown in Figure 9 in METHOD FOR CONTINUOUS DETERMINATION culture solution, the results showed that: pyridine degradable bacteria TJKYBD-01
Apparent bioaugmentation is played, and the pyridine that will contain about in 48h in the coal chemical industrial waste water of 1000mg/L pyridine is complete
Removal.
The pyridine degradable bacteria TJKYBD-01 of present embodiment can be using pyridine as sole carbon source, nitrogen source and the energy
Growth and breeding is carried out, it is in 48h that the pyridine degradable of 1000mg/L is complete.Pyridine degradable bacteria TJKYBD-01 is resistant to concentration height
Up to the pyridine of 1500mg/L, there is excellent degradation effect to higher concentration pyridine industrial wastewater, it is useless which can be applied to coking
The biological reinforced processing of water, coal chemical industrial waste water and petrochemical wastewater, has a extensive future.
Claims (5)
1. a pyridine degradation bacterium strain Rhodococcus sp (Rhodococcus sp.) TJKYBD-01, deposit number CGMCC
NO.10175 is resistant to coking wastewater of the pyridine at concentrations up to 1500mg/L, coal chemical industrial waste water or petrochemical wastewater.
2. the microbial inoculum containing pyridine degradable bacteria Rhodococcus sp (Rhodococcus sp.) TJKYBD-01 described in claim 1.
3. pyridine degradable bacteria Rhodococcus sp (Rhodococcus sp.) TJKYBD-01 described in claim 1 or claim 2 institute
Application of the microbial inoculum stated in purified industrial waste water.
4. application as claimed in claim 3, which is characterized in that the industrial wastewater be coking wastewater, coal chemical industrial waste water and
One of petrochemical wastewater.
5. application as described in claim 3 or 4, which is characterized in that the bacterial strain or microbial inoculum can be using pyridines as sole carbon
Source, nitrogen source and the energy carry out growth and breeding, in 48h by coking wastewater, coal chemical industrial waste water or petrochemical wastewater≤
The pyridine degradable of 1000mg/L is complete.
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| CN106318891B (en) * | 2016-10-27 | 2019-07-02 | 安徽师范大学 | Microbial inoculum and the application of one pyridine degradation bacterium strain strain a5 and its production |
| CN114940961B (en) * | 2022-06-21 | 2023-09-15 | 太原理工大学 | Salt-tolerant pyridine degradation strain and application thereof in high-salt pyridine wastewater |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481673A (en) * | 2009-01-13 | 2009-07-15 | 北京未名凯拓农业生物技术有限公司 | Pyridine degradable bacteria, complex bacterial agent thereof, preparation and use |
| CN101857846A (en) * | 2010-04-20 | 2010-10-13 | 深圳清华大学研究院 | Rhodococcus erythropolis and microbial inoculum and application thereof |
| CN102154172A (en) * | 2011-01-13 | 2011-08-17 | 中国农业大学 | Heterotrophic-nitrification and aerobic-denitrification Rhodococuus pyridinivorans and use thereof |
| CN105039212A (en) * | 2015-07-09 | 2015-11-11 | 中国海洋石油总公司 | Rhodococcus strain C3, microbial agent containing rhodococcus strain C3, and applications of rhodococcus strain C3 and microbial agent |
-
2015
- 2015-12-31 CN CN201511034489.5A patent/CN105462901B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481673A (en) * | 2009-01-13 | 2009-07-15 | 北京未名凯拓农业生物技术有限公司 | Pyridine degradable bacteria, complex bacterial agent thereof, preparation and use |
| CN101857846A (en) * | 2010-04-20 | 2010-10-13 | 深圳清华大学研究院 | Rhodococcus erythropolis and microbial inoculum and application thereof |
| CN102154172A (en) * | 2011-01-13 | 2011-08-17 | 中国农业大学 | Heterotrophic-nitrification and aerobic-denitrification Rhodococuus pyridinivorans and use thereof |
| CN105039212A (en) * | 2015-07-09 | 2015-11-11 | 中国海洋石油总公司 | Rhodococcus strain C3, microbial agent containing rhodococcus strain C3, and applications of rhodococcus strain C3 and microbial agent |
Non-Patent Citations (1)
| Title |
|---|
| "红球菌Chr-9降解吡啶和苯酚的特性";孙纪全等;《应用与环境生物学报》;20120825;第18卷(第4期);第647-650页 * |
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