CN105255758B - Sulfobacillus acidophilus TPY is used for the purposes and method of degradation of phenol pollutant - Google Patents
Sulfobacillus acidophilus TPY is used for the purposes and method of degradation of phenol pollutant Download PDFInfo
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- 230000015556 catabolic process Effects 0.000 title claims abstract description 23
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 23
- 239000003344 environmental pollutant Substances 0.000 title claims abstract description 16
- 231100000719 pollutant Toxicity 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title abstract description 16
- 241000020554 Sulfobacillus acidophilus TPY Species 0.000 title abstract description 5
- 241000521591 Sulfobacillus acidophilus Species 0.000 claims abstract description 36
- 239000002351 wastewater Substances 0.000 claims abstract description 15
- 239000007640 basal medium Substances 0.000 claims abstract description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 229940041514 candida albicans extract Drugs 0.000 claims description 2
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- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000001311 chemical methods and process Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
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- ODLMAHJVESYWTB-UHFFFAOYSA-N propylbenzene Chemical compound CCCC1=CC=CC=C1 ODLMAHJVESYWTB-UHFFFAOYSA-N 0.000 description 2
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
Sulfobacillus acidophilus TPY is used for the purposes and method of degradation of phenol pollutant, is related to Sulfobacillus acidophilus.Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY can be used for degradation of phenol pollutant.The phenol pollutant can be high temperature and highly acid industrial pollution waste water containing phenol.Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY is inoculated in basal medium, is added after phenol culture and completes the degradation of phenol pollutant.Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY growth temperature is higher, and growth pH value is lower, can be applied to acid, the high temperature waste water of pollutant containing phenol processing.
Description
Technical field
The present invention relates to Sulfobacillus acidophilus, more particularly to a kind of Sulfobacillus acidophilus TPY for degrading
The purposes and method of phenol pollutant.
Background technique
Phenol is a kind of aromatic organic compounds, has volatile white crystal, there is special smell, and ordering is stone
Charcoal acid, molecular formula C6H5OH.Phenol is extract from coal tar, and the main path of synthesizing phenol is different at present
The oxidation of propylbenzene.Industrially, phenol is primarily present in the waste water of petroleum, petrochemical industry, coking and production phenol, main to use
In (phenol hydroxyls of the efficient Phenol hydroxylase gene GXP04 of [1] Tang Lili such as production explosive, fertilizer, paint, rubber and synthetic resin
Change the clone Guangxi University of enzyme gene cluster；2005).Phenol belongs to highly toxic substance, a large amount of discharges of wastewater containing phenol, especially
The waste water of some high temperature and highly acid, processing are difficult.Phenol can by with mucocutaneous contact, absorption and oral intrusive body
It is interior and make one to be poisoned.Slow poisoning can cause headache, appetite stimulator, nausea etc.；Acute poisoning sucking high concentration phenol can lead to
Headache, out of strength, blurred vision, pulmonary edema etc.；Wrongly take cause alimentary canal to be burnt, gastric-intestinal perforation, respiratory failure.On the other hand, work
The phenol wastewater that phenol generates is produced in industry can inhibit the growth of aqueous bio, and the fish and phenol for eating carbdism pollute crops
It can cause human body vomiting and diarrhea, thus human lives and ecological environment are caused to seriously threaten ([2] pair tinkling of pieces of jades phenol degrading
The screening of bacterium and its research Northeastern University of characteristic；2009).
Due to the discharge of phenol wastewater, existence water source of the drinking water of the mankind and aquatile etc. contains by potential threat
The wastewater treatment of phenol generally has physical-chemical process and bioanalysis, wherein physical-chemical process include solvent extraction ([3] Dai Youyuan,
Yang Yiyan, Yang Tian avenge Complexation Extraction Process Treating Phenolic Wastewater technology chemical industry progress 1991:40), but common solvent extraction work
Skill Phenol-Containing Wastewater Treatment is extremely difficult to discharge standard；Chemical oxidization method ([4] Christoskova S, Stoyanova
M.Degradation of phenolic waste waters over Ni-oxide.Water Res 2001；35:2073),
Because consuming a large amount of oxidant H2O2And higher cost；Wet oxidation process is (in [5] Lin Chunmian, Jin Yaomen, Pan Zhi man of virtue and ability's supercritical water
The oxygenolysis colleges and universities chemical engineering journal 1998:87 of phenol), this method mesohigh, high temperature cost are very high；There are also photochemistry
Oxidizing process, photochemical catalysis oxidizing process, input cost is higher, and ([6] Zhang Naidong, Huang Junli, Zheng Wei strengthens UV/Fenton method
The research Techniques and Equipment for Environmental Pollution Control 2002 of phenol in degradation water；3:20).In view of cost and practical application effect,
These methods are all difficult to be widely used in wastewater containing phenol processing, the especially waste water of high temperature and highly acid in China.Bioanalysis
Mostly use Aerobic Process for Treatment, anaerobic-aerobic treatment, activated sludge and biofilm.Since biological treatment is usually to utilize microorganism
It degrades, at low cost for physico-chemical process, treating capacity is big, more economical effective and without secondary pollution.
The microorganism of degradation of phenol includes some bacteriums, such as from hair monad (Comamonas testosteroni)
([7]
Arai H,Akahira S,Ohishi T,Kudo T.Adaptation of Comamonas testosteroni
TA441to utilization of phenol by spontaneous mutation of the gene for a
trans-acting factor.Molecular Microbiology 1999；33:1132), pseudomonas
(Pseudomonas sp)([8]Powlowski J,Shingler V.Genetics and biochemistry of
phenol degradation by Pseudomonas sp.CF600.Biodegradation1994；5:219), Rhod
(Rhodococcus)([9]Szokol J,Rucka L,Simcikova M,Halada P,Nesvera J,Patek
M.Induction and carbon catabolite repression of phenol degradation genes in
Rhodococcus erythropolis and Rhodococcus jostii.Appl Microbiol Biotechnol
2014；98:8267), acinetobacter (Acinetobacter) ([10] Zhan Y, Yu H, Yan Y, Chen M, Lu W, Li
S,et al.Genes involved in the benzoate catabolic pathway in Acinetobacter
calcoaceticus PHEA-2.Curr Microbiol 2008；57:609) etc., one of the most common Phenol-degrading Bacteria Strains are false
Monad and acinetobacter calcoaceticus；Some fungies also degradation of phenol ([11] Semple KT, Cain RB.Biodegradation of
phenols by the alga Ochromonas danica.Appl Environ Microbiol1996；62:1265).This
Outside, some higher plants and algae also degradation of phenol ([12] Gibson DT.Microbial degradation of
aromatic compounds.Science 1967；161:1093).In soil environment, aromatic series can be largely metabolized by existing
The microorganism of compound, the compound of a variety of benzene classes of gram-positive bacteria bacillus energy metabolism, Gram-negative bacteria produce alkali bar
Bacterium such as Bacillus foecalis alkaligenes (Alcaligenes faecalis) ([13] Rehfuss M, Urban J.Alcaligenes
faecalis subsp.phenolicus subsp.nov.a phenol-degrading,denitrifying bacterium
isolated from a graywater bioprocessor.Syst Appl Microbiol 2005；It is 28:421) also aerobic
Change the function of phenyl ring, these microorganisms can be using phenol as carbon source for growth.
The applicant provides Sulfobacillus acidophilus in Chinese patent CN201110026855.8
(Sulfobacillus acidophilus) TPY, the bacterium are from nero deep hydrothermal solution mouth (12.2`29``N, 104.2`01``
W, 3083 meters of the depth of water) it is separated to, China typical culture collection center (address: Chinese was preserved on August 16th, 2010
Wuhan Wuhan University), deposit number is CCTCC No:M 2010203.
Summary of the invention
The purpose of the present invention is to provide a kind of Sulfobacillus acidophilus (Sulfobacillus acidophilus)
The purposes of TPY.
Another object of the present invention is to provide a kind of Sulfobacillus acidophilus (Sulfobacillus
Acidophilus) the method for TPY degradation of phenol pollutant.
Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY was protected on August 16th, 2010
It is hidden in China typical culture collection center (address: the Chinese Wuhan Wuhan University), deposit number is CCTCC No:M
2010203 (see the Chinese patent CN201110026855.8 of the applicant).
The bacterium be it is isolated from nero deep hydrothermal solution mouth (12.2`29``N, 104.2`01``W, 3083 meters of the depth of water),
The bacterium is in rod-short, is gram-positive bacteria, and size is (0.3~0.5) μ m (1~3) μm；It can utilize ferrous salt, list
Matter sulphur autophyting growth can also utilize yeast powder, glucose, peptone, the organic matters heterotrophic growth such as glycerol, 16S rDNA gene
Nucleotide sequence length be 1533bp, GenBank accession number be NR.074781.1.
Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY can be used for degradation of phenol pollution
Object.The phenol pollutant can be high temperature and highly acid industrial pollution waste water containing phenol.
The side of Sulfobacillus acidophilus (Sulfobacillus acidophilus) the TPY degradation of phenol pollutant
Method, the specific steps are as follows:
Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY is inoculated in basal medium, is added
Enter to complete after phenol culture the degradation of phenol pollutant.
The composition of the basal medium can are as follows: 3g/L (NH4)2SO4, 0.5g/L K2HPO4, 0.5g/L MgSO4, 0.1g/
L KCl, 0.01g/L Ca (NO3)2, 13.9g/L FeSO4·7H2O, 0.2g/L yeast extract.
The mass concentration of the phenol can be 90~120mg/L；The condition of the culture can are as follows: and pH value is 1.5~4.0,
The temperature of culture is 40~50 DEG C, and shaking speed is 180~220r/min, 38~42h of shake culture.
Using the concentration of high performance liquid chromatography detection phenol.
There are many microorganism of degradation of phenol, but there has been no the reports about Sulfobacillus acidophilus degradation of phenol so far
Road, the certain density phenol and Sulfobacillus acidophilus TPY can degrade, and can, pH value lower ring higher in temperature
It is grown in border, therefore, can be applied to the processing of high temperature and highly acid industrial pollution waste water containing phenol, it is advantageous that degradation
It is high-efficient, at low cost and without secondary pollution.
Compared with prior art, of the invention to have the prominent advantages that: Sulfobacillus acidophilus (Sulfobacillus
Acidophilus) TPY growth temperature is higher, and growth pH value is lower, can be applied to acid, the high temperature waste water of pollutant containing phenol
Processing.
Detailed description of the invention
Fig. 1 is that phenol concentration calculates standard curve.
Fig. 2 is Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY after cultivating different time
Phenol degrading situation.
Specific embodiment
The foundation of embodiment 1, phenol Standard curve
It accurately weighs 1g phenol to be dissolved in 100mL deionized water, with 0.22 μm of membrane filtration, is made into 0.01g/mL's
Mother liquor, then the mother liquor of accurate 2 μ L of volume aspirated, 5 μ L, 7 μ L, 10 μ L, 15 μ L, 20 μ L, 25 μ L, 30 μ L are distinguished to total with pipettor
Volume is in the aseptic deionized water of 1mL.High effective liquid chromatography for measuring is used after mixing, using phenol concentration as abscissa, with peak
Area is that ordinate draws standard curve.High performance liquid chromatograph model Agilent 1200, chromatographic column are C18 column, chromatographic column
Gauge size is 4.6 × 150mm, and mobile phase is methanol and water (50: 50, v/v), flow velocity 0.8mL/min, 1 μ L of sample volume, detection
Wavelength 270nm.The standard curve measured is as shown in Figure 1, curve equation is y=1.1835x+1.4448, R2=0.9998, y are
The peak area of measurement, x are phenol concentration (μ g/L).
The degradation of embodiment 2, Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY Pyrogentisinic Acid
Phenol degrading experiment is divided to two groups of progress, i.e. experimental group and control group.Experimental group: the acidophilus of fresh activation vulcanizes gemma
Bacillus (Sulfobacillus acidophilus) TPY is inoculated in the inoculum concentration of 5% (v/v) to be trained containing basis described in 100mL
In the 250mL conical flask for supporting base, the phenol of 0.01% (w/v) is added, phenol passes through 0.22 μm of membrane filtration；Control group: contain
Have in the 250mL conical flask of basal medium described in 100mL, add the phenol of 0.01% (w/v), phenol passes through 0.22 μm of filter
Film filtering, is not inoculated with bacterium.Initial pH is modulated to 1.8, and cultivation temperature is 45 DEG C, and 200r/min shaking table shake culture is being cultivated
14h, 19h, for 24 hours, 29h, 38h, 40h sample detection phenol degrading utilization power, and according to 1 curve equation of embodiment calculate phenol
Concentration.The result shows that phenol is degradable in culture 40h, blank control group phenol concentration is essentially unchanged (Fig. 2).
Claims (3)
1. Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY is applied in degradation of phenol pollutant；Institute
It states Sulfobacillus acidophilus (Sulfobacillus acidophilus) TPY and was preserved in Chinese allusion quotation on August 16th, 2010
Type culture collection, deposit number are CCTCC No:M 2010203；
The phenol pollutant is the industrial pollution waste water containing phenol, by Sulfobacillus acidophilus (Sulfobacillus
Acidophilus) TPY is inoculated in basal medium, completes the degradation of phenol pollutant；The composition of the basal medium
Are as follows: 3g/L (NH4)2SO4, 0.5g/L K2HPO4, 0.5g/L MgSO4, 0.1g/L KCl, 0.01g/L Ca (NO3)2, 13.9g/
LFeSO4·7H2O, 0.2g/L yeast extract；
The condition of the culture are as follows: pH value is 1.5~4.0, and the temperature of culture is 40~50 DEG C.
2. application as described in claim 1, it is characterised in that the mass concentration of the phenol is 90~120mg/L.
3. application as described in claim 1, it is characterised in that the condition of the culture are as follows: shaking speed is 180~220r/min,
38~42h of shake culture.
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| CN102154336A (en) * | 2011-01-25 | 2011-08-17 | 国家海洋局第三海洋研究所 | Acidophilic vulcanized bacillus thioredoxin reductase gene and recombinant protein thereof |
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| CN102154336A (en) * | 2011-01-25 | 2011-08-17 | 国家海洋局第三海洋研究所 | Acidophilic vulcanized bacillus thioredoxin reductase gene and recombinant protein thereof |
Non-Patent Citations (3)
| Title |
|---|
| CP0029010.1-Sulfobacillus acidophilus TPY，complete genome;Li B et al.;《GenBank》;20140130;第1-4页，尤其是2-4页 * |
| Phenol degradation by Sulfobacillus acidophilus TPY via the meta-pathway;Wengen Zhou et al.;《Microbiological Research》;20160514;第190卷;第37-45页 * |
| 一株深海中等嗜热嗜酸菌的分离及鉴定;漆辉洲等;《海洋学报》;20090331;第31卷(第2期);第152-158页，尤其是摘要和材料 * |
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