CN106754358A - A kind of co-culture of cells blake bottle of convenient transfer cell - Google Patents
A kind of co-culture of cells blake bottle of convenient transfer cell Download PDFInfo
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- CN106754358A CN106754358A CN201611206503.XA CN201611206503A CN106754358A CN 106754358 A CN106754358 A CN 106754358A CN 201611206503 A CN201611206503 A CN 201611206503A CN 106754358 A CN106754358 A CN 106754358A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a kind of co-culture of cells blake bottle of convenient transfer cell,The bottle of blake bottle is divided into Liang Ge culturing room by distance member,Culture bottleneck is respectively arranged with the side wall of each culturing room,The closed cover of culture bottleneck connection sealing bottleneck,The distance member is provided with the means of communication that can connect Liang Ge culturing room,The means of communication sets the closing office that can separate Liang Ge culturing room path,The component for variable-pressure that can change culturing room's air pressure inside is additionally provided with the side wall of the culturing room,The bottom surface of culturing room is double-decker,It in culturing room is elastic film that superstructure is,Understructure is the rigid support plate below elastic film,Close closed cover closing bottle,And the component for variable-pressure of culturing room make in culturing room produce negative pressure condition under,Elastic film swells,Closed cavity is formed between elastic film and rigid support plate.The Tissue Culture Flask can realize the co-cultivation of cell, while the cell in convenient transfer bottle.
Description
Technical field
The present invention relates to a kind of cell culture apparatus, and in particular to a kind of co-culture of cells blake bottle of convenient transfer cell.
Background technology
Tissue Culture Flask is a kind of device for cultured cells.Glass and plastics, bottom can be divided into according to material
Portion's shape has square, rectangle, triangle etc., has 5 to 500ml selective according to bottle volume.It is posed to due to its bottom surface
Cell attachment area is usually used in greatly the relatively large culture of cell than conventional culture dish area, and Tissue Culture Flask is typically provided with bottle
Body, sets a tiny bottleneck on bottle side wall, and bottle cap is set on bottleneck, and the bottle cap of domestic Tissue Culture Flask is generally envelope
The closed cover for closing, can be used for closed culture, meanwhile, typically using threaded connection, unscrewing bottle cap just can ensure bottle for bottle cap and bottleneck
Interior gas is exchanged with gas in environment, it can also be used to open culture.Cell in Tissue Culture Flask is adherent in bottle body bottom
Culture is, it is necessary to culture medium, passage routine operation are such as changed in the various operations in cell cultivation process is required for dropper
Or liquid-transfering gun pipette tips from tiny bottleneck stretch into bottle in operate, and in operation in order to avoid pollution, dropper and pipette tips
To avoid touching bottleneck as far as possible, operating process is more bothered, especially during passage etc. needs transfer cell, carefully
Born of the same parents depart from blake bottle bottom surface, it is necessary to first absorb culture in glassware base, and rinsing cell surface with PBS removes residual media, then adds
Enter to move enzymic digestion certain hour, be subsequently adding new band blood serum medium and stop digestion, and with dropper draw culture medium constantly wash away,
Piping and druming blake bottle bottom surface, makes cell come off to form cell suspension from bottle bottom surface, and the bottleneck yet with blake bottle is smaller, drop
Motion of the pipe in blake bottle receives very big limitation, and culture medium is difficult the cell of the corner location for brushing bottle bottom surface again, gives
Whole cell transfer process brings difficulty.In addition, the interaction of different cells is that living nature generally occurs.Cell
Co-cultivation is to study the basic means that cell interacts.With the increase to RESEARCH ON CELL-BIOLOGY depth, iuntercellular is mutual
Effect actually belongs to a kind of intercellular information interchange.This information interchange has a great impact to cell, affects them
Propagation, differentiation, material secretion.Therefore co-culture of cells has profound influence to RESEARCH ON CELL-BIOLOGY, however, cell is trained altogether
It is not that simple different cells are placed in same culturing room is cultivated to support, different cell co-cultivations, different cell growth cycles
Different, a kind of cell is likely to grow the living space of another cell of too fast contention, is not easy to observation；Mixed culture cell
Also inconvenience is distinguished, and is caused cell in-situ to be identified and be there is certain difficulty, and the simple only one of which of existing Cell culture bottle structure
Culturing room, it is impossible to preferably realize the co-incubation of cell.
The content of the invention
It is an object of the invention to be improved to existing product, there is provided a kind of co-culture of cells training of convenient transfer cell
Support bottle.This blake bottle can be while cell be in respective culturing room individually culture conveniently observation observation, according to experiment
Need the exchange for realizing cell secreta at any time to exchange, realize intercellular information interchange, realize co-culture of cells, additionally it is possible to
Cell makes the attached cell of blake bottle bottom be easily disengaged from whole bottle bottom surface when shifting, and makes the experimental implementation for needing to shift cell
It is more convenient.
To achieve these goals, the technical scheme that the present invention is provided is as follows：
A kind of co-culture of cells blake bottle of convenient transfer cell, the bottle of the blake bottle is divided into two trainings by distance member
Support room, be respectively arranged with culture bottleneck on the side wall of each culturing room, the closed cover of culture bottleneck connection sealing bottleneck, it is described between
Being provided with every part can connect the means of communication of Liang Ge culturing room, and the means of communication is set can separate Liang Ge culturing room path
Closing office, is additionally provided with the component for variable-pressure that can change culturing room's air pressure inside, the bottom of culturing room on the side wall of the culturing room
Face is double-decker, and it in culturing room is elastic film that superstructure is, understructure is below elastic film
Rigid support plate, closes closed cover closing bottle, and the component for variable-pressure of culturing room makes in culturing room under generation negative pressure condition, elasticity
Film is swelled, and closed cavity is formed between elastic film and rigid support plate.
The Tissue Culture Flask will be divided into two cultures by the distance member with switchable means of communication inside bottle
Room, Liang Ge culturing room is each provided with the bottleneck with closed cover, when the means of communication on distance member is closed, between two culturing room
It is separate, it is independent of each other in the different cells of Liang Ge culturing room culture, can be in itself growth demand and experiment according to cell
Need to change liquid by the bottleneck complete independently of each culturing room（Change culture medium）, passage etc. regular growth experimental implementation, without shadow
The cell growth of another culturing room of sound.Needed in passage etc. in the operation for shift cell, complete the step of moving enzymic digestion and add
After entering enough culture mediums, only need to be in the case of closed cover closing bottle to be closed, the component for variable-pressure using culturing room changes repeatedly
Culture room pressure, component for variable-pressure reduces culture room pressure, and the elastic film of culturing room bottom surface stretches grand under suction function
Rise, elastic film recovery of shape when component for variable-pressure renewal cultivation room pressure or increase air pressure so changes repeatedly in change press mechanism
While becoming culture room pressure, the whole elastic film of cell adherence（Including edge）Fluctuated with the change of air pressure, made
Cell through moving enzymic digestion comes off from elastic film, while become press mechanism being also resulted in during culture room pressure is changed
The disturbance of bottle interior air-flow, airflow function in culture medium makes the fully dispersed formation cell suspension of the cell in culture medium, completes
After aforesaid operations, need to only use dropper to draw cell suspension can complete cell transfer operation, without as in traditional operation with drop
Pipe draws culture medium and constantly washes away, blows and beats blake bottle bottom surface, and cell is come off from bottle bottom surface could obtain cell suspension,
Effectively prevent because the bottleneck of blake bottle is smaller, motion of the dropper in blake bottle receives very big limitation, and culture medium is difficult
The operating difficulties of the cell of the corner location of bottle bottom surface is brushed again.Meanwhile, when cell carries out letter between two culturing room of experiment needs
During breath exchange, the means of communication on distance member can be opened, the path phase that the culture medium in Liang Ge culturing room passes through means of communication
Counterdiffusion, exchange, realize that the exchange of cell secreta is exchanged, and so as to realize intercellular information interchange, are trained altogether with reaching cell
Foster purpose, if attached cell is cultivated in two training interiors, in cellular communication interaction process, different cells are still
It is in respective culturing room without being mutually mixed, convenient observation, observation of cell upgrowth situation and pattern change, meanwhile, thin
During born of the same parents co-culture, the closed cover closing blake bottle of two culturing room is closed, using becoming press mechanism Shi Liang culturing room bottom surface
Elastic film slight fluctuations appropriate time, may also speed up being mutually mixed for culture medium between culturing room, between enhancing cell secreta
Interaction, advantageously in Germicidal efficacy.
Specifically, described distance member is arranged at bottle center, bottle is divided into left and right Liang Ge culturing room, each
The bottleneck of culturing room may be contained within the side wall away from distance member, and the component for variable-pressure is arranged at the front side wall of each culturing room
On.
Specifically, the distance member includes to be divided into inside bottle the dividing plate of Liang Ge culturing room, the lower partition is close to
Bottle bottom surface sets the through hole of two culturing room of connection, and the distance member also includes that the inserted sheet of through hole can be closed, on the dividing plate
It is provided with the slot matched with inserted sheet, the port of slot is located at dividing plate top, the slot runs through through hole, inserted sheet is from slot end
In mouth insertion dividing plate, the distance member is additionally provided with fixed inserted sheet position to close or open the inserted sheet detent mechanism of through hole,
The distance member is additionally provided with the sealing mechanism for preventing culturing room's gas leakage.
Specifically, through hole side port is coated with miillpore filter, the membrane aperture of the miillpore filter is micro- less than 50
Rice.The miillpore filter aperture is set allows nutrient solution to be freely accessible to, but does not allow cell to pass in and out so that it is thin that two culturing room cultivate
Even if born of the same parents are when culture is suspended, still ensure that different cells are in single culturing room without being mutually mixed, facilitate cell experiment
Operation and observation, observation.
Specifically, the inserted sheet detent mechanism includes being installed on the baffle plate at the top of inserted sheet, the size of the baffle plate bottom surface is big
In the size of the inserted sheet top surface being attached thereto, baffle plate is located at outside bottle, the end at the top of the upper surface correspondence slot of the bottle
Mouth sets opening, and the opening shape size is identical with slot top port, and inserted sheet passes bottle from the opening of the upper surface of bottle
Body connects baffle plate, and the sealing mechanism for preventing culturing room's gas leakage is rubber seal at the opening of the upper surface of bottle
Circle, the rubber seal is brought into close contact with inserted sheet side wall；The flexible supporting mechanism of baffle plate setting and compression mechanism, elasticity
Baffle plate is supported in bottle top by supporting mechanism in its natural state, and inserting above through hole is limited in the inserted sheet that baffle plate is connected
In groove, through hole is opened, and two culturing room connection, the culture medium in culturing room is exchanged with each other by through hole, realizes cellular communication；
When described compression mechanism can oppress elastic supporting mechanism compressive deformation, baffle plate is set to press close to bottle upper surface, in compression mechanism
Under pressure effect, baffle plate drives inserted sheet insertion slot bottom lock through hole, the path that now cell culture medium is exchanged to be closed, and two
Culturing room is relatively independent, is independent of each other in the different cells of Liang Ge culturing room culture, can be in itself growth demand according to cell
Need to change the regular growth experimental implementation such as liquid, sample-adding, passage by the bottleneck complete independently of each culturing room with experiment.
Specifically, the elastic supporting mechanism of the baffle plate is symmetrically arranged two springs of baffle bottom, the spring
One end connection baffle plate bottom surface, the spring other end connects the upper surface of bottle, and spring does not suffer oppression mechanism pressure when acting on, by baffle plate
Bottle top is supported in, is limited in the slot above through hole with the inserted sheet that baffle plate is connected, through hole is opened；The pressure of the baffle plate
Compeling mechanism includes being placed on the baffle plate sheath outside baffle plate, and the baffle plate sheath is arranged on bottle upper surface, the baffle plate sheath
Baffle plate jack is provided with, baffle plate passes the upper surface of baffle plate sheath from baffle plate jack, and the edge of baffle plate sheath upper surface is fixed
One end of rubber band is connected, the edge away from the baffle plate sheath upper surface of rubber band fixing end sets fixed rubber band free end
Hook, under rubber band extended state, the free end coupling hook of rubber band, rubber band be disposed through baffle plate top set
There is groove to oppress baffle plate, spring-compressed, inserted sheet insertion slot bottom lock through hole to bottle direction.
Specifically, the slot bottom is provided with rubber sheet gasketing, the via bottoms and bottle bottom surface（Inner surface）
Spacing be 1-2 millimeters.The design of rubber sheet gasketing causes that inserted sheet contacts even closer with slot bottom, plays the close of replacing
Envelope buffer action, in cell cultivation process, the Culture liquid measure in blake bottle is relatively limited, presses close to 1-2 millimeters of bottle bottom surface and sets
The through hole of meter may insure the mutual circulation of culture medium.
Specifically, the component for variable-pressure for changing culturing room's air pressure inside includes rubber wind ball, the rubber wind ball
The passage that air entry is connected in bottle side wall is connected with culturing room.Compressing rubber wind ball, makes the gas in wind ball
Discharge, now closes closed cover, then loosens wind ball, and the air entry of wind ball makes culture Indoor Air from culture indoor suction
Pressure reduces, and the elastic film of culturing room bottom surface stretches protuberance under suction function, again compress wind ball, gas punching in spheroid
Enter in culturing room, culture room pressure recovers, and elastic film recovery of shape is so repeated several times, and makes elastic film（Including edge
Place）Fluctuated with the change of air pressure, make to be come off from elastic film through moving the cell of enzymic digestion, while rubber wind ball changes culture
The disturbance of bottle interior air-flow is also result in during room pressure, airflow function makes the cell in culture medium abundant in culture medium
Dispersion forms cell suspension, and completing cell after completing aforesaid operations, by only need to drawing cell suspension with dropper shifts behaviour
Make, it is simple and convenient.
Specifically, the rigid support plate of the upper surface of the bottle, side wall and bottom surface lower floor of culturing room is clear hard
Plastic construction, the elastic film on the upper strata of culturing room bottom surface is PDMS transparent elastic films, the edge of the elastic film
It is fixedly connected with rigid support plate below, confined space is formed between elastic film and rigid support plate.PDMS is transparent
Elastic membrane has preferably elasticity and translucency, can meet optical microscope inspection cell needs in cell cultivation process.Institute
It is 150-300 microns to state the thickness of elastic film, and it is preferably elastic that inventor has found that the PDMS film of this thickness has, and
It is hardly damaged during tensile deformation repeatedly, can Reusability, it can be ensured that the service life of the Tissue Culture Flask.The culture
The gross thickness of room bottom surface double-decker is 0.8-1.2 millimeters, can meet the observation needs of optical fiber mirror.
Beneficial effects of the present invention are：The invention provides a kind of co-culture of cells blake bottle of convenient transfer cell.This
Kind of blake bottle can need at any time while cell is in respective culturing room individually culture convenient observation observation according to experiment
Realize that the exchange of cell secreta is exchanged, realize intercellular information interchange, realize co-culture of cells, additionally it is possible in cell transfer
When the attached cell of blake bottle bottom is easily disengaged from whole bottle bottom surface, make to need to shift the experimental implementation of cell more just
It is prompt.Specifically, the Tissue Culture Flask will be divided into two by the distance member with switchable means of communication inside bottle
Culturing room, Liang Ge culturing room is each provided with the bottleneck with closed cover, when the means of communication on distance member is closed, two cultures
It is separate between room, Liang Ge culturing room culture different cells be independent of each other, can be in itself according to cell growth demand and
Experiment needs to change liquid by the bottleneck complete independently of each culturing room（Change culture medium）, passage etc. regular growth experimental implementation, and
The cell growth of another culturing room is not influenceed.Needed in passage etc. in the operation for shift cell, complete to move the step of enzymic digestion
Suddenly after adding enough culture mediums, only need to close closed cover closing bottle in the case of, using culturing room component for variable-pressure repeatedly
Change culture room pressure, component for variable-pressure reduces culture room pressure, and the elastic film of culturing room bottom surface is drop-down in suction function
Elastic film recovery of shape when stretching protuberance, component for variable-pressure renewal cultivation room pressure or increase air pressure, it is so anti-in change press mechanism
While multiple change culture room pressure, the whole elastic film of cell adherence（Including edge）The ripple with the change of air pressure
It is dynamic, make to be come off from elastic film through moving the cell of enzymic digestion, while change press mechanism is during culture room pressure is changed
The disturbance of bottle interior air-flow is also result in, airflow function makes the fully dispersed formation cell of the cell in culture medium suspended in culture medium
Liquid, after completing aforesaid operations, only need to draw cell suspension with dropper can complete cell transfer operation, without as traditional operation
Middle dropper draws culture medium and constantly washes away, blow and beat blake bottle bottom surface, and cell is come off from bottle bottom surface, could to obtain cell outstanding
Turbid liquid, also effectively prevent because the bottleneck of blake bottle is smaller, and motion of the dropper in blake bottle receives very big limitation, culture
Base is difficult the operating difficulties of the cell of the corner location for brushing bottle bottom surface again.Meanwhile, when cell between two culturing room of experiment needs
When carrying out information interchange, the means of communication on distance member can be opened, the culture medium in Liang Ge culturing room is by means of communication
The counterdiffusion of path phase, exchange, realize that the exchange of cell secreta is exchanged, thin to reach so as to realize intercellular information interchange
The purpose that born of the same parents co-culture is different in cellular communication interaction process if attached cell is cultivated in two training interiors
Cell is still in respective culturing room without being mutually mixed, convenient observation, observation of cell upgrowth situation and pattern change, together
When, during co-culture of cells, the closed cover closing blake bottle of two culturing room is closed, using becoming press mechanism Shi Liang culturing room
The elastic film slight fluctuations appropriate time of bottom surface, may also speed up being mutually mixed for culture medium between culturing room, enhancing cell point
Interaction between secretion, advantageously in Germicidal efficacy.
Brief description of the drawings
Fig. 1 is the structural representation of the culturing room's segmented state of Tissue Culture Flask of the present invention two；
Fig. 2 is the AA of Fig. 1 to profile；
Fig. 3 is the big figure of B prescriptions of Fig. 1；
Fig. 4 is the profile of dividing plate；
Fig. 5 is the structural representation of the culturing room's connected state of Tissue Culture Flask of the present invention two；
Fig. 6 is the A ' A ' of Fig. 5 to profile；
In figure：1 bottle, 2 culturing room, 3 closed covers, 4 dividing plates, 5 inserted sheets, 6 rubber bands；7 grooves, 8 through holes, 9 rubber sheet gasketings,
10 hooks, 11 rubber wind balls, 12 elastic films, 13 springs, 14 miillpore filters, 15 slots, 16 baffle plate sheaths, 17 baffle plates, 18 gears
Plate jack.
Specific embodiment
For the technical characterstic for illustrating this programme can be understood, below by a specific embodiment, and its accompanying drawing is combined, it is right
This programme is illustrated.
As shown in Figures 1 to 6, a kind of co-culture of cells blake bottle of convenient transfer cell, the quilt of bottle 1 of the blake bottle
Distance member is divided into Liang Ge culturing room 2, and culture bottleneck, culture bottleneck connection are respectively arranged with the side wall of each culturing room 2
The closed cover 3 of bottleneck is sealed, the distance member is provided with the means of communication that can connect Liang Ge culturing room 2, the means of communication
Setting can separate the closing office of the path of Liang Ge culturing room 2, and being additionally provided with the side wall of the culturing room 2 can change culturing room 2
The component for variable-pressure of air pressure inside, the bottom surface of culturing room 2 is double-decker, and it in culturing room is elastic film that superstructure is
12, understructure is the rigid support plate positioned at the lower section of elastic film 12, the closing closing bottle 2 of closed cover 3, and culturing room 1
Component for variable-pressure makes in culturing room under generation negative pressure condition, and elastic film 12 swells, between elastic film 12 and rigid support plate
Form closed cavity.
Specifically, described distance member is arranged at the center of bottle 1, and bottle 1 is divided into left and right Liang Ge culturing room 2,
The bottleneck of each culturing room 2 may be contained within the side wall away from distance member, before the component for variable-pressure is arranged at each culturing room 2
On the wall of side.The distance member includes to be divided into inside bottle 1 dividing plate 4 of Liang Ge culturing room 2, and the bottom of dividing plate 4 is near bottle 1
Bottom surface sets the through hole 8 of two culturing room 2 of connection, and the distance member also includes that the inserted sheet 5 of through hole 8 can be closed, on the dividing plate 4
The slot 15 matched with inserted sheet 5 is provided with, the port of slot 15 is located at the top of dividing plate 4, and the slot 15 runs through through hole 8, inserts
From the port of slot 15 insertion dividing plate 4, the distance member is additionally provided with the position of fixed inserted sheet 5 to close or open through hole 8 piece 5
Inserted sheet detent mechanism, the distance member is additionally provided with the sealing mechanism for preventing the gas leakage of culturing room 2.The side of the through hole 8 one
Miillpore filter 14,30 microns of the membrane aperture of the miillpore filter are coated with mouthful.The inserted sheet detent mechanism is slotting including being installed on
The baffle plate 17 at the top of piece 5, the size of the bottom surface of the baffle plate 17 is more than the size of the top surface of inserted sheet 5 being attached thereto, and baffle plate 17 is located at
Outside bottle 1, the port at the top of the upper surface of the bottle 1 correspondence slot 15 sets opening, the opening shape size with insert
The top port of groove 15 is identical, and inserted sheet 5 passes the connection baffle plate 17 of bottle 1 from the opening of the upper surface of bottle 1, described to prevent culturing room
The sealing mechanism of 2 gas leakage is the rubber seal at the opening of the upper surface of bottle 1, the rubber seal and inserted sheet
5 side walls are brought into close contact；The baffle plate 17 is provided with elastic supporting mechanism and compression mechanism, and elastic supporting mechanism is in its natural state
Baffle plate 17 is supported in the top of bottle 1, the inserted sheet 5 being connected with baffle plate 17 is limited in the slot 15 of the top of through hole 8, through hole 8
Open；Described compression mechanism can oppress elastic supporting mechanism compressive deformation, baffle plate 17 is pressed close to the upper surface of bottle 1, in compressor unit
Under the pressure effect of structure, baffle plate 17 drives the insertion bottom lock through hole 8 of slot 15 of inserted sheet 5.The resilient support machine of the baffle plate 17
Structure is two springs 13 that the bottom symmetrical of baffle plate 17 is set, and one end connection baffle plate 17 bottom surface of the spring 13, spring 13 is another
The upper surface of end connection bottle 1, spring 13 does not suffer oppression mechanism pressure when acting on, and baffle plate 17 is supported in into the top of bottle 1, with gear
The connected inserted sheet 5 of plate 17 is limited in the slot 15 of the top of through hole 8, and through hole 8 is opened；The compression mechanism of the baffle plate 17 includes
The baffle plate sheath 16 outside baffle plate 17 is placed on, the baffle plate sheath 16 is arranged on the upper surface of bottle 1, and the baffle plate sheath 16 sets
Baffle plate jack 18 is equipped with, baffle plate 17 passes the upper surface of baffle plate sheath 16, the side of the upper surface of baffle plate sheath 16 from baffle plate jack 18
One end of rubber band 6 is fixedly connected at edge, the edge away from the upper surface of baffle plate sheath 16 of the fixing end of rubber band 6 sets fixation
The hook 10 of the free end of rubber band 6, under the extended state of rubber band 6, the free end coupling hook 10 of rubber band 6, rubber band 6 is worn
Cross to be arranged at the top of baffle plate 17 and be provided with groove 7 to the direction of bottle 1 compressing baffle plate 17, spring 13 compresses, the insertion slot 15 of inserted sheet 5
Bottom lock through hole 8.The bottom of the slot 15 is provided with rubber sheet gasketing 9, between the bottom of the through hole 8 and the bottom surface of bottle 1
Away from being 1.5 millimeters.The component for variable-pressure for changing the air pressure inside of culturing room 2 includes rubber wind ball 11, the rubber wind ball 11
Air entry be connected in the passage of the side wall of bottle 1 and connected with culturing room 2.The upper surface of the bottle 1, side wall and culture
The rigid support plate of the bottom surface lower floor of room 2 is clear hard plastic construction, and the elastic film 12 on the upper strata of the bottom surface of culturing room 2 is
PDMS transparent elastic films, the edge of the elastic film 12 is fixedly connected with rigid support plate below, in elasticity
Confined space is formed between film 12 and rigid support plate.The thickness of the elastic film 12 is 150-300 microns, the culture
The gross thickness of the bottom surface double-decker of room 2 is 0.8-1.2 millimeters.
Illustrative embodiments listed above are only not to technical scheme described in the invention for understanding that the present invention is used
Restriction, about the those of ordinary skill in field, on the basis of technical scheme described in claim, various changes can also be made
Change or deform, all equivalent changes or deformation should all be covered within claims of the invention.The present invention is not
Part is described in detail in detail, the known technology of those skilled in the art of the present technique is.
Claims (10)
1. a kind of convenience shifts the co-culture of cells blake bottle of cell, it is characterised in that the bottle of the blake bottle（1）It is spaced
Part is divided into Liang Ge culturing room（2）, each culturing room（2）Side wall on be respectively arranged with culture bottleneck, culture bottleneck connection
Seal the closed cover of bottleneck（3）, the distance member is provided with can connect Liang Ge culturing room（2）Means of communication, the connection
Mechanism is set can separate Liang Ge culturing room（2）The closing office of path, the culturing room（2）Side wall on be additionally provided with and can change
Culturing room（2）The component for variable-pressure of air pressure inside, culturing room（2）Bottom surface be double-decker, superstructure is in culturing room
Be elastic film（12）, understructure is positioned at elastic film（12）The rigid support plate of lower section, closes closed cover（3）Closing
Bottle（2）, and culturing room（1）Component for variable-pressure make in culturing room produce negative pressure condition under, elastic film（12）Protuberance, in elasticity
Film（12）Closed cavity is formed and rigid support plate between.
2. a kind of convenience as claimed in claim 1 shifts the co-culture of cells blake bottle of cell, it is characterised in that between described
Bottle is arranged at every part（1）Center, by bottle（1）It is divided into left and right Liang Ge culturing room（2）, each culturing room（2）Bottleneck
May be contained within the side wall away from distance member, the component for variable-pressure is arranged at each culturing room（2）Front side wall on.
3. a kind of convenience as claimed in claim 1 shifts the co-culture of cells blake bottle of cell, it is characterised in that the interval
Part is included bottle（1）Inside is divided into Liang Ge culturing room（2）Dividing plate（4）, dividing plate（4）Bottom is near bottle（1）Bottom surface
Two culturing room of connection are set（2）Through hole（8）, the distance member is also including that can close through hole（8）Inserted sheet（5）, it is described every
Plate（4）On be provided with and inserted sheet（5）The slot for matching（15）, slot（15）Port be located at dividing plate（4）Top, the slot
（15）Through through hole（8）, inserted sheet（5）From slot（15）Insert dividing plate in port（4）In, the distance member is additionally provided with fixed inserting
Piece（5）Position is closing or open through hole（8）Inserted sheet detent mechanism, the distance member is additionally provided with and prevents culturing room（2）
The sealing mechanism of gas leakage.
4. a kind of convenience as claimed in claim 3 shifts the co-culture of cells blake bottle of cell, it is characterised in that the through hole
（8）Side port is coated with miillpore filter（14）, the membrane aperture of the miillpore filter is less than 50 microns.
5. a kind of convenience as claimed in claim 3 shifts the co-culture of cells blake bottle of cell, it is characterised in that the inserted sheet
Detent mechanism includes being installed on inserted sheet（5）The baffle plate at top（17）, the baffle plate（17）The size of bottom surface is more than what is be attached thereto
Inserted sheet（5）The size of top surface, baffle plate（17）Positioned at bottle（1）Outside, the bottle（1）Upper surface correspondence slot（15）Top
Port opening, the opening shape size and slot are set（15）Top port is identical, inserted sheet（5）From bottle（1）Upper table
The opening in face passes bottle（1）Connection baffle plate（17）, it is described to prevent culturing room（2）The sealing mechanism of gas leakage is installed in bottle
（1）Upper surface opening at rubber seal, the rubber seal and inserted sheet（5）Side wall is brought into close contact；The baffle plate
（17）Elastic supporting mechanism and compression mechanism are provided with, elastic supporting mechanism is in its natural state by baffle plate（17）It is supported in bottle
（1）Top, with baffle plate（17）Connected inserted sheet（5）It is limited in through hole（8）The slot of top（15）In, through hole（8）Open；Institute
The compression mechanism stated can oppress elastic supporting mechanism compressive deformation, make baffle plate（17）Press close to bottle（1）Upper surface, in compression mechanism
Pressure effect under, baffle plate（17）Drive inserted sheet（5）Insertion slot（15）Bottom lock through hole（8）.
6. a kind of convenience as claimed in claim 5 shifts the co-culture of cells blake bottle of cell, it is characterised in that the baffle plate
（17）Elastic supporting mechanism be baffle plate（17）Two springs that bottom symmetrical is set（13）, the spring（13）One end connection
Baffle plate（17）Bottom surface, spring（13）The other end connects bottle（1）Upper surface, spring（13）The mechanism pressure that do not suffer oppression is acted on
When, by baffle plate（17）It is supported in bottle（1）Top, with baffle plate（17）Connected inserted sheet（5）It is limited in through hole（8）Top insert
Groove（15）In, through hole（8）Open；The baffle plate（17）Compression mechanism include be placed on baffle plate（17）Outside baffle plate sheath
（16）, the baffle plate sheath（16）Installed in bottle（1）On upper surface, the baffle plate sheath（16）It is provided with baffle plate jack
（18）, baffle plate（17）From baffle plate jack（18）Pass baffle plate sheath（16）Upper surface, baffle plate sheath（16）The edge of upper surface
Place is fixedly connected rubber band（6）One end, away from rubber band（6）The baffle plate sheath of fixing end（16）The edge of upper surface is set
Fixed rubber band（6）The hook of free end（10）, in rubber band（6）Under extended state, the free end coupling hook of rubber band
（10）, rubber band（6）It is disposed through baffle plate（17）Top is provided with groove（7）To bottle（1）Oppress baffle plate in direction（17）, bullet
Spring（13）Compression, inserted sheet（5）Insertion slot（15）Bottom lock through hole（8）.
7. a kind of convenience as claimed in claim 3 shifts the co-culture of cells blake bottle of cell, it is characterised in that the slot
Bottom is provided with rubber sheet gasketing（9）, the through hole（8）Bottom and bottle（1）The spacing of bottom surface is 1-2 millimeters.
8. a kind of convenience as claimed in claim 1 shifts the co-culture of cells blake bottle of cell, it is characterised in that described to change
Become culturing room（2）The component for variable-pressure of air pressure inside includes rubber wind ball（11）, the rubber wind ball（11）Air entry connection set
It is placed in bottle（1）The passage of side wall and culturing room（2）Connection.
9. a kind of convenience as claimed in claim 1 shifts the co-culture of cells blake bottle of cell, it is characterised in that the bottle
（1）Upper surface, side wall and culturing room（2）The rigid support plate of bottom surface lower floor is clear hard plastic construction, culturing room（2）
The elastic film on the upper strata of bottom surface（12）It is PDMS transparent elastic films, the elastic film（12）Edge with below
Rigid support plate be fixedly connected, in elastic film（12）Confined space is formed and rigid support plate between.
10. a kind of convenience as claimed in claim 9 shifts the co-culture of cells blake bottle of cell, it is characterised in that the bullet
Property film（12）Thickness be 150-300 microns, the culturing room（2）The gross thickness of bottom surface double-decker is 0.8-1.2 millimeters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611206503.XA CN106754358B (en) | 2016-12-23 | 2016-12-23 | Cell co-culture bottle convenient for transferring cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611206503.XA CN106754358B (en) | 2016-12-23 | 2016-12-23 | Cell co-culture bottle convenient for transferring cells |
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| Publication Number | Publication Date |
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| CN106754358A true CN106754358A (en) | 2017-05-31 |
| CN106754358B CN106754358B (en) | 2023-06-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611206503.XA Active CN106754358B (en) | 2016-12-23 | 2016-12-23 | Cell co-culture bottle convenient for transferring cells |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108728359A (en) * | 2018-06-08 | 2018-11-02 | 天晴干细胞股份有限公司 | A kind of application of space station Tissue Culture Flask and its auxiliary device |
| CN112980662A (en) * | 2021-02-20 | 2021-06-18 | 郭明 | Cell collection device capable of obtaining multiple samples for hematology department |
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| CA2193810A1 (en) * | 1994-06-28 | 1996-01-11 | John Wilson | Compartmentalized tissue culture flask |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108728359B (en) * | 2018-06-08 | 2022-03-11 | 天晴干细胞股份有限公司 | Space station cell culture bottle and application of auxiliary device thereof |
| CN112980662A (en) * | 2021-02-20 | 2021-06-18 | 郭明 | Cell collection device capable of obtaining multiple samples for hematology department |
| CN112980662B (en) * | 2021-02-20 | 2022-07-01 | 青岛市黄岛区中心医院 | Cell collection device capable of obtaining multiple samples for hematology department |
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|---|---|
| CN106754358B (en) | 2023-06-30 |
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