CN106727857A - It is a kind of that the preparation method for preventing and treating hyperuricemia and gout medicine-food two-purpose monomer is extracted from Semen Cuscutae - Google Patents
It is a kind of that the preparation method for preventing and treating hyperuricemia and gout medicine-food two-purpose monomer is extracted from Semen Cuscutae Download PDFInfo
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- CN106727857A CN106727857A CN201610996958.XA CN201610996958A CN106727857A CN 106727857 A CN106727857 A CN 106727857A CN 201610996958 A CN201610996958 A CN 201610996958A CN 106727857 A CN106727857 A CN 106727857A
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- semen cuscutae
- food
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- eluent
- extraction
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Classifications
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/43—Cuscutaceae (Dodder family), e.g. Cuscuta epithymum or greater dodder
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
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- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The preparation method for preventing and treating hyperuricemia and gout medicine-food two-purpose monomer is extracted from Semen Cuscutae the present invention relates to a kind of, after Semen Cuscutae raw material is smashed, ethanol is added to be heated to reflux carrying out coarse extraction, then purified using macroporous resin column, and test and analyze eluent with thin-layered chromatography, collect the eluent with accounting target compound high and merge, gained eluent is finally prepared into product.Semen Cuscutae of the present invention contains phenylpropanoids, and monomer of the present invention is made up of double parallel tetrahydrofuran ring connection glycosyls, and such as n-butanol, ethyl acetate dissolubility are preferable in medium polar solvent, and with certain water solubility.The present invention is decocted using 60% ethanol and extracted, and polar macroporous purifying resin, autotelic enrichment subject monomers compound group, low cost, high income easily realizes industrialization and scale.Capsule, tablet, health care medicinal tea etc. prepared by the invention, convenient to takeization, with diversification feature.
Description
Technical field
The present invention relates to a kind of preparation method of medicine-food two-purpose monomer, and in particular to it is high that one kind extracts preventing and treating from Semen Cuscutae
The preparation method of uricacidemia and gout medicine-food two-purpose monomer.
Background technology
The report of Chinese medicine hyperuricemia and primary gout is more.In clinical practice and daily life, people
Find that some can treat the effective single medicinal material of hyperuricemia, wherein the active component of many medicines and pharmacology are made
With being elucidated with.As Bi Collettii, the bark of ash, Bai Ai, globe artichoke, the root of Chinese clematis, plantain seed, golden cypress, desmodium, bark of ash etc. have certain
Anti-trioxypurine is acted on[1,2].Research display, barrenwort, coix seed, rhizoma alismatis, plantain seed, bark of ash can increase uric acid excretion, and bark of ash may be used also
Increase the dissolving of uric acid；Herba Lycopi, Radix Angelicae Sinensis can suppress uric acid synthesis.It is another to there is research to show that the bark of ash, Asiatic plantain, rheum officinale, rhizoma atractylodis promote
Uric acid is excluded from kidney, and rheum officinale energy uricosuric is excreted from stool；Lily, edible tulip etc. are acted on colchicinoid sample, can
Suppress leucocyte chemotaxis, so as to mitigate the inflammatory reaction of urarthritis；Waxgourd peel, the shell of areca nut, the root bark of white mulberry, dried orange peel, wood
The promoting the circulation of qi such as perfume, fuling peel profit water medicine can improve endogenous creatinine clearance rate, increase urine volume, discharge uric acid, reduce blood uric acid[3,4]。
Semen Cuscutae is convolvulaceous plant Cuscuta australis (Cuscuta australis R.Br.) or Semen Cuscutae (Cuscuta
Chinensis Lam.) dry mature seed, sweet, temperature returns kidney,liver,spleen to pass through, with nourishing liver and kidney, astringent sperm contracting urine, it is antiabortive,
The effect of improving eyesight, antidiarrheal.The main chemical compositions of Semen Cuscutae are resin-like glucoside, cholesterine, campesterol
(Campesterol), sitosterol, stigmasterol and triterpene acids, carbohydrate etc..Semen Cuscutae contains abundant chemical composition, in kidney tonifying
Establishing-Yang, improve immunocompetence, anti-aging, liver-nourishing and eyesight-improving, prevent miscarriage, adjust hypothalamic-pituitary-ovarian axis function, adjust endocrine,
Treatment angiocardiopathy, the aspects such as gonadal hormone sample effect that promote have extensive pharmacological action.Semen Cuscutae has contracting urine with anti-something lost
Urine is acted on, and enters kidney channel, " kidney governing water metabolism ", sweet kidney tonifying, and kidney qi is then taken the photograph effectively admittedly enough, makes bladder folding degree of having, and takes charge of urine drains；
Enter liver the spleen channel, aqueous that The spleen has the function to transport and transform nutrients, liver assists aqueous metabolism.Semen Cuscutae compound gives mouse stomach, can reduce urination amount and subtract
Few number of micturitions.
Treatment by Chinese herbs hyperuricemia and gout it is evident in efficacy, but Chinese medicine Shortcomings show several in use
Individual aspect：1st, conventional preparation and instructions of taking are inconvenient, such as decocting method, taste weight when taking, taking, and during because of personal decoction
Between it is different with decocting method, curative effect can not be completely secured；2nd, taking dose is big, decocts or directly crush the formulation for preparing, composition
Composition includes active principle and invalid component, and taking dose is big, it is impossible to reach the effect that Special Nursing in Special Disease is controlled, and is possible to produce poison
Side effect；3rd, monomeric compound low yield, high cost are obtained with modern separating and purifying technology, is difficult to realize scale and industry
Change；4th, realize that monomeric compound group still has certain technical difficulty, and high cost by modern synthetic technology.
[1] woods ultramarine, Xie Jian, king sea east ends extract solution to experimental study [J] of hyperuricemia rat China wind in vain
Diseases caused by dampness magazine, 2005,9 (8):509-510.
[2] Lin Fengping, appoints enlightenment, the root ofs Chinese clematis such as Song Enfeng to experimental study [J] Chinese patent drugs of UN rat,
2006,28(6):842-845.
[3] Lee's table, Sun Lin modern times Kidney Disease [M] Nanchang:In Jiangxi Science technology publishing house, 2000,537-
543.
[4] Shen Qing methods tcm clinical practices nephrology [M] Shanghai:Shanghai scientific and technical literature publishing house, 1998,206-
211.
The content of the invention
For the problem of above-mentioned Chinese medicine Shortcomings performance in use, the present invention provides that a kind of consumption is small, uses
Convenience, few adverse reaction, low cost, effect substantially, be easy to industrialized and prevent and treat hyperuricemia and gout medicine-food two-purpose monomer
Preparation method.
The purpose of the present invention will be achieved through the following technical solutions：One kind from Semen Cuscutae extract prevent and treat hyperuricemia and
The preparation method of gout medicine-food two-purpose monomer, comprises the following steps：
Step one, coarse extraction：After Semen Cuscutae raw material is smashed, the raw material of certain mass is weighed, add 20 times of 60% second of amount
After alcohol is heated to reflux 2h, the dregs of a decoction are isolated, 15 times of 60% ethanol of amount will be added to be heated to reflux 1.5h in the dregs of a decoction, merge extract solution,
High speed centrifugation, discards insoluble matter, and vacuum distillation ethanol obtains crude extract；
Step 2, chromatography：By AB-8 types macroporous resin column on crude extract in step one, Static Adsorption 2 hours,
10BV water elutions are used, eluent is discarded, then is eluted successively from low to high with different concentration ethanol, as extract solution；
Step 3, detection and analysis：Whether contain list during the extract solution in step 2 is differentiated into eluent with thin-layered chromatography
A certain monomer and its relative amount in body compound group, collect the eluent with accounting target compound high and merge, by institute
Obtain eluent and be prepared into product.
Preferably, described a kind of extraction from Semen Cuscutae prevents and treats the preparation of hyperuricemia and gout medicine-food two-purpose monomer
Method, in the step one, Semen Cuscutae includes Semen Cuscutae glycosides A, Semen Cuscutae glycosides B, new Semen Cuscutae glycosides A, new Semen Cuscutae glycosides B, new Tu
One or more in the sub- glycosides C of silk.
Preferably, described a kind of extraction from Semen Cuscutae prevents and treats the preparation of hyperuricemia and gout medicine-food two-purpose monomer
Method, in the step 3, the product of eluent processing includes capsule, tablet, mixture, food additives and health protection tea.
Preferably, described a kind of extraction from Semen Cuscutae prevents and treats the preparation of hyperuricemia and gout medicine-food two-purpose monomer
Method, the preparation method of the capsule is：Vacuum concentration, is vacuum dried or is spray-dried and to obtain fine powder, plus 6 times of 20% starch of amount
Paste, pelletizes encapsulated.
Preferably, described a kind of extraction from Semen Cuscutae prevents and treats the preparation of hyperuricemia and gout medicine-food two-purpose monomer
Method, the preparation method of the tablet is：Gained eluent is vacuum dried or is spray-dried to obtain fine powder, adds 6 times of amounts 20% to form sediment
Paste mixing, 1 times of amount 10%PVP (PVP) ethanol, granulation, drying, compressing tablet.
Preferably, described a kind of extraction from Semen Cuscutae prevents and treats the preparation of hyperuricemia and gout medicine-food two-purpose monomer
Method, the preparation method of the mixture is：Gained eluent concentrates or is diluted to the medicinal extract of relative density 1.2, and bottling makes mouth
Take liquid.
Preferably, described a kind of extraction from Semen Cuscutae prevents and treats the preparation of hyperuricemia and gout medicine-food two-purpose monomer
Method, the preparation method of the food additives is：Gained eluent is concentrated in vacuo, and is vacuum dried or is spray-dried and to obtain fine powder,
Can be used as food additives.
Preferably, described a kind of extraction from Semen Cuscutae prevents and treats the preparation of hyperuricemia and gout medicine-food two-purpose monomer
Method, the preparation method of the food additives is：The concentration of gained eluent is the medicinal extract of relative density 1.2, you can used as guarantor
Health food additive.
Preferably, described a kind of extraction from Semen Cuscutae prevents and treats the preparation of hyperuricemia and gout medicine-food two-purpose monomer
Method, the preparation method of the health protection tea is：Gained eluent is concentrated under reduced pressure into the medicinal extract of relative density 1.2, with extra-strong tea
Leaf presses 1：25 mass ratio mixing, dries 8 hours under the conditions of being placed in 55 DEG C, then is ground into 20 mesh, and sterilization packaging storage is made 6g
The anti-trioxypurine health protection tea of one bag.
The beneficial effects of the invention are as follows：Semen Cuscutae of the present invention contains phenylpropanoids, monomer of the present invention
It is made up of double parallel tetrahydrofuran ring connection glycosyls, such as n-butanol, ethyl acetate dissolubility are preferable in medium polar solvent, and
And with certain water solubility.The present invention is decocted using 60% ethanol and extracted, polar macroporous purifying resin, autotelic enrichment target
Monomeric compound group, low cost, high income easily realizes industrialization and scale.Capsule, tablet, guarantor prepared by the invention
Strong medicinal tea etc., convenient to takeization, with diversification feature.
Brief description of the drawings
Fig. 1 is the pathological anatomy figure of the blank group of the embodiment of the present invention 6；
Fig. 2 is the pathological anatomy figure of the model group of the embodiment of the present invention 6；
Fig. 3 is the pathological anatomy figure of the high dose group of the embodiment of the present invention 6.
Specific embodiment
To more fully understand the present invention, further specific elaboration is made to the present invention below by following examples, but not
Limitation of the invention is can be regarded as, it is nonessential according to some that foregoing invention content is made for those skilled in the art
Improve and adjust, be also considered as being within the scope of the present invention.
Embodiment 1, the preparation based on capsule of the present invention
1) bulk drug Semen Cuscutae is weighed, 3# hungry area softgel shells are some standby；
2) raw medicinal material adds 20 times of amount 60% ethanol cold soaking bubble 1d, heating and refluxing extraction 2h, the dregs of a decoction to add 15 times of 60% second of amount
Alcohol is heated to reflux 1.5h, merges extract solution, and ethanol is recovered under reduced pressure, and high speed centrifugation concentrate obtains filtrate, upper AB-8 types macropore tree
Fat post, Static Adsorption 2 hours, uses 10BV water elutions, discards eluent, then is eluted successively from low to high with different concentration ethanol,
With whether, containing a certain monomer and its relative amount in monomeric compound group, collection has high in thin-layered chromatography discriminating eluent
The eluent of accounting target compound simultaneously merges, and is vacuum dried or is spray-dried and to obtain fine powder, adds 6 times of 20% gelatinized corn starches of amount,
Granulation, drying loads 3# hungry area softgel shells, every content-filled about 0.32g of hungry area softgel shell, clean, select grain, is made appropriate packaging rule
The finished product of lattice.
Embodiment 2, the preparation based on Tablets
1) bulk drug Semen Cuscutae is weighed standby；
2) raw medicinal material adds 20 times of amount 60% ethanol cold soaking bubble 1d, heating and refluxing extraction 2h, the dregs of a decoction to add 15 times of 60% second of amount
Alcohol is heated to reflux 1.5h, merges extract solution, and ethanol is recovered under reduced pressure, and high speed centrifugation concentrate obtains filtrate, upper AB-8 types macropore tree
Fat post, Static Adsorption 2 hours, uses 10BV water elutions, discards eluent, then is eluted successively from low to high with different concentration ethanol,
With whether, containing a certain monomer and its relative amount in monomeric compound group, collection has high in thin-layered chromatography discriminating eluent
The eluent of accounting target compound simultaneously merges, and is vacuum dried or is spray-dried and to obtain fine powder, add 5 times of 20% gelatinized corn starches of amount, 1 times
Amount 10%PVP (PVP) ethanol, granulation, drying, the tabletted heavy for about former piece of 0.4g, sugar coating, chip select is made suitable
When the finished product of packing specification.
Embodiment 3, the preparation based on mixture of the present invention
1) bulk drug Semen Cuscutae is weighed, 10ml size oral liquid bottles and bottle cap are standby；
2) raw medicinal material adds 20 times of amount 60% ethanol cold soaking bubble 1d, heating and refluxing extraction 2h, the dregs of a decoction to add 15 times of 60% second of amount
Alcohol is heated to reflux 1.5h, merges extract solution, and ethanol is recovered under reduced pressure, and high speed centrifugation concentrate obtains filtrate, upper AB-8 types macropore tree
Fat post, Static Adsorption 2 hours, uses 10BV water elutions, discards eluent, then is eluted successively from low to high with different concentration ethanol,
With whether, containing a certain monomer and its relative amount in monomeric compound group, collection has high in thin-layered chromatography discriminating eluent
The eluent of accounting target compound simultaneously merges, and is concentrated into relative density 1.2, adds sweetener, preservative, fining agent etc., puts
Put, filter, take filtrate, sterilize, the filling oral liquid into 10ml/ branch rolls lid, is made the finished product of appropriate packing specification.
Embodiment 4, the preparation based on food supplement of the present invention
1) bulk drug Semen Cuscutae is weighed standby；
2) raw medicinal material adds 20 times of amount 60% ethanol cold soaking bubble 1d, heating and refluxing extraction 2h, the dregs of a decoction to add 15 times of 60% second of amount
Alcohol is heated to reflux 1.5h, merges extract solution, and ethanol is recovered under reduced pressure, and high speed centrifugation concentrate obtains filtrate, upper AB-8 types macropore tree
Fat post, Static Adsorption 2 hours, uses 10BV water elutions, discards eluent, then is eluted successively from low to high with different concentration ethanol,
With whether, containing a certain monomer and its relative amount in monomeric compound group, collection has high in thin-layered chromatography discriminating eluent
The eluent of accounting target compound simultaneously merges, and is concentrated into relative density 1.2, sterilizing, appropriate containers sealing, as health food
Additive；Or vacuum concentration, it is vacuum dried or is spray-dried and to obtain fine powder, appropriate containers sealing, as food supplement.
Embodiment 5, the preparation based on health protection tea of the present invention
1) bulk drug Semen Cuscutae is weighed, extra-strong tea is some standby；
2) raw medicinal material adds 20 times of amount 60% ethanol cold soaking bubble 1d, heating and refluxing extraction 2h, the dregs of a decoction to add 15 times of 60% second of amount
Alcohol is heated to reflux 1.5h, merges extract solution, and ethanol is recovered under reduced pressure, and high speed centrifugation concentrate obtains filtrate, upper AB-8 types macropore tree
Fat post, Static Adsorption 2 hours, uses 10BV water elutions, discards eluent, then is eluted successively from low to high with different concentration ethanol,
With whether, containing a certain monomer and its relative amount in monomeric compound group, collection has high in thin-layered chromatography discriminating eluent
The eluent of accounting target compound simultaneously merges, and is concentrated into relative density 1.2, and 1 is pressed with Tie Guanyin tea：25 mass ratioes mix,
Dried 8 hours under the conditions of being placed in 55 DEG C, then be ground into 20 mesh, sterilized, be made the anti-trioxypurine health protection tea of the bags of 6g mono-.
Embodiment 6, the present invention improve gouty nephropathy pathology
Experimental animal：KM mouse, SPF grades, male, body weight 18-22g is carried by Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center
For.Normal raising supplies examination after 3 days.
Medicine and reagent：Semen Cuscutae (Guangzhou Medicinal Material Co., Ltd. Traditional Chinese Medicinal Tablet Factory).
It is prepared by product of the present invention：Raw medicinal material Semen Cuscutae adds 20 times of amounts 60% ethanol cold soaking bubble 1d, heating and refluxing extraction 2h,
The dregs of a decoction add 15 times of 60% ethanol of amount to be heated to reflux 1.5h, merge extract solution, and ethanol is recovered under reduced pressure, and high speed centrifugation concentrate must be filtered
Liquid, upper AB-8 types macroporous resin column, Static Adsorption 2 hours uses 10BV water elutions, discards eluent, then use different concentration ethanol
Elute successively from low to high, whether a certain monomer and its phase in monomeric compound group are contained in differentiating eluent with thin-layered chromatography
To content, collect the eluent with accounting target compound high and merge, be vacuum dried or be spray-dried and to obtain fine powder, face use
Preceding distilled water is configured to required concentration.
KM mouse 80 are taken, four groups are equally divided into, fasting 10h before gavage prohibits water 6h.Daily gavage twice, every time
0.6ml, continuous 14 days, observes 14 days again after drug withdrawal.Blank group of the present invention：Give equivalent distilled water；Model group：Allopurinol
0.04g/kg/d is administered, high dose group：The product dosage is 6.00g/kg/d, equivalent to 3000 times of people's consumption.
Result of the test：(1) blank group (10)：As shown in Figure 1, cortex renis internal glomerulus is evenly distributed, under 10 times of object lens
Numbers of glomeruli is 15~20, and renal cells distribution is complete, and cast is had no in renal tubule tube chamber, and renal interstitial has no out
Blood, cell infiltration and urate crystal.(2) model group (10)：As shown in Figure 2, cortex renis numbers of glomeruli has been reduced,
Numbers of glomeruli is 5~10 under 10 times of object lens, and renal tubule stove shape atrophy is shown in protein cast in indivedual renal tubule tube chambers, has no
Uric acid mineralization, renal interstitial is shown in bleeding and Focal Lymphocytic Infiltration.(3) high dose group (10)：From the figure 3, it may be seen that glomerulus
Reduction degree, renal tubule atrophy are light compared with model group, and cast and urate crystal are had no in renal tubule tube chamber, and renal interstitial has no
Bleeding and lymphocytic infiltration.
Conclusion (of pressure testing)：The monomer of the present invention can improve gouty nephropathy
Embodiment 7, subchronic toxicity of the present invention experiment
Experimental animal：KM mouse, SPF grades, male, body weight 18-22g is carried by Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center
For.Normal raising supplies examination after 3 days.
Medicine and reagent：Semen Cuscutae (Guangzhou Medicinal Material Co., Ltd. Traditional Chinese Medicinal Tablet Factory).
It is prepared by product of the present invention：Raw medicinal material adds 20 times of amount 60% ethanol cold soaking bubble 1d, heating and refluxing extraction 2h, the dregs of a decoction to add
15 times of 60% ethanol of amount are heated to reflux 1.5h, merge extract solution, and ethanol is recovered under reduced pressure, and high speed centrifugation concentrate obtains filtrate, on
AB-8 type macroporous resin columns, Static Adsorption 2 hours, use 10BV water elutions, discard eluent, then with different concentration ethanol by as little as
Whether height is eluted successively, differentiates in eluent containing a certain monomer in monomeric compound group and its relative to contain with thin-layered chromatography
Amount, collects the eluent with accounting target compound high and merges, and is vacuum dried or is spray-dried and to obtain fine powder, before use with steaming
Distilled water is configured to required concentration.
KM mouse 80 are taken, four groups are equally divided into, fasting 10h before gavage prohibits water 6h.Daily gavage twice, every time
0.6ml, continuous 14 days, observes 14 days again after drug withdrawal.Medicine small dose group of the present invention：Dosage is 0.06g/kg/d, equivalent to people
30 times of consumption；Medicine middle dose group of the present invention：Dosage is 0.60g/kg/d, equivalent to 300 times of people's consumption；Medicine of the present invention is big
Dosage group：Dosage is 6.00g/kg/d, equivalent to 3000 times of people's consumption；Control group：Give equivalent distilled water.
Result of the test：Appetite is reduced in each group mouse species administration 5h, and activity weakens, medicine color semiliquid stool etc., extensive after drug withdrawal
It is multiple normal.Average weight of each test group when the 14th day after 14d being administered and being discontinued is compared with control group without significant difference (P>
0.05).Erythrocytes, total white blood cells, content of hemoglobin, the platelet count of test group mouse after drug withdrawal the 1st day and
After drug withdrawal the 14th day compared with control group without significant difference (P>0.05).The serum glutamic pyruvic transminase of each test group mouse, paddy
Careless transaminase, alkaline phosphatase after the drug withdrawal the 1st day and after being discontinued the 14th day compared with control group without significant difference (P>0.05).
The 1st day and the 14th day after drug withdrawal, cut open inspection is shown in that the form of each group heart, liver, spleen, lung, kidney, stomach and intestines, color, quality are normal.Disease
Reason histological examination.High, medium and low dosage group of the invention and control group mice are showed no obvious pathological change.
By experiment, high, medium and low dosage group of the invention is above clinical treatment dosage, is not found in the corresponding course for the treatment of
Any subchronic toxicity reaction.With reference to acute toxicity test, its consumption also has no that any poison is secondary equivalent to 3000 times of people's consumption
Reaction, therefore the present invention can be used as preventing and treating hyperuricemia, the dual-purpose formula of gout medicinal health food and preparation.
Conclusion (of pressure testing)：The subchronic toxicity test of the present invention shows safety non-toxic of the present invention.
Embodiment 8, acute toxicity test of the present invention
Experimental animal：KM mouse, SPF grades, male, body weight 18-22g is carried by Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center
For.Normal raising supplies examination after 3 days.
Medicine and reagent：Semen Cuscutae (Guangzhou Medicinal Material Co., Ltd. Traditional Chinese Medicinal Tablet Factory).
It is prepared by product of the present invention：Raw medicinal material adds 20 times of amount 60% ethanol cold soaking bubble 1d, heating and refluxing extraction 2h, the dregs of a decoction to add
15 times of 60% ethanol of amount are heated to reflux 1.5h, merge extract solution, and ethanol is recovered under reduced pressure, and high speed centrifugation concentrate obtains filtrate, on
AB-8 type macroporous resin columns, Static Adsorption 2 hours, use 10BV water elutions, discard eluent, then with different concentration ethanol by as little as
Whether height is eluted successively, differentiates in eluent containing a certain monomer in monomeric compound group and its relative to contain with thin-layered chromatography
Amount, collects the eluent with accounting target compound high and merges, and is vacuum dried or is spray-dried and to obtain fine powder, before use with steaming
Distilled water is configured to required concentration.
KM mouse 80 are taken, four groups are equally divided into, fasting 10h before gavage prohibits water 6h.Daily gavage twice, every time
0.6ml, continuous 7 days.Medicine small dose group of the present invention：Dosage is 0.06g/kg/d, equivalent to 30 times of people's consumption；Medicine of the present invention
Middle dose group：Dosage is 0.60g/kg/d, equivalent to 300 times of people's consumption；Drug bolus group of the present invention：Dosage is 6.00g/
Kg/d, equivalent to 3000 times of people's consumption；Control group：Give equivalent distilled water.
Result of the test：7 days mouse of experiment are all survived, and appetite is reduced in rarely seen animal administration 5h, and activity weakens, medicine color
Semiliquid stool etc., recovers normal after drug withdrawal.4 groups of animals drinking-water, hair color, body weight, excrement and the heart, liver, spleen, lung, kidney, thymus gland, brain,
The aspects such as form, size, the smoothness of main organs such as tongue, stomach, intestines, bladder, sexual gland (uterus, ovary or testis and epididymis) are
No significant difference.Described above, toxic and side effect of the present invention is small, and because by drug concentration and volumetric constraint, can not find and cause small
The dead dosage of mouse, therefore LD50 of the invention cannot be measured.
Experiment conclusion：The acute toxicity test of the present invention shows safety non-toxic of the present invention.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (9)
1. a kind of that the preparation method for preventing and treating hyperuricemia and gout medicine-food two-purpose monomer is extracted from Semen Cuscutae, its feature exists
In comprising the following steps：
Step one, coarse extraction：After Semen Cuscutae raw material is smashed, the raw material of certain mass is weighed, add 20 times of 60% ethanol of amount to add
After heat backflow 2h, the dregs of a decoction are isolated, 15 times of 60% ethanol of amount will be added to be heated to reflux 1.5h in the dregs of a decoction, merge extract solution, at a high speed
Centrifugation, discards insoluble matter, and vacuum distillation ethanol obtains crude extract；
Step 2, chromatography：AB-8 types macroporous resin column on crude extract in step one, Static Adsorption 2 hours are used
10BV water elutions, discard eluent, then are eluted successively from low to high with different concentration ethanol, as extract solution；
Step 3, detection and analysis：Whether contain singulation during extract solution thin-layered chromatography in step 2 is differentiated into eluent
A certain monomer and its relative amount in compound group, collect the eluent with accounting target compound high and merge, and gained is washed
De- liquid is prepared into product.
2. a kind of extraction from Semen Cuscutae according to claim 1 prevents and treats hyperuricemia and gout medicine-food two-purpose monomer
Preparation method, it is characterised in that：In the step one, Semen Cuscutae includes Semen Cuscutae glycosides A, Semen Cuscutae glycosides B, new Semen Cuscutae glycosides A, new
One or more in Semen Cuscutae glycosides B, new Semen Cuscutae glycosides C.
3. a kind of extraction from Semen Cuscutae according to claim 1 prevents and treats hyperuricemia and gout medicine-food two-purpose monomer
Preparation method, it is characterised in that：In the step 3, the product of eluent processing includes capsule, tablet, mixture, food additive
Plus agent and health protection tea.
4. a kind of extraction from Semen Cuscutae according to claim 3 prevents and treats hyperuricemia and gout medicine-food two-purpose monomer
Preparation method, it is characterised in that the preparation method of the capsule is：Fine powder is concentrated in vacuo, is vacuum dried or is spray-dried and to obtain,
Plus 6 times of 20% gelatinized corn starches of amount, pelletize encapsulated.
5. a kind of extraction from Semen Cuscutae according to claim 3 prevents and treats hyperuricemia and gout medicine-food two-purpose monomer
Preparation method, it is characterised in that the preparation method of the tablet is：Gained eluent is vacuum dried or is spray-dried to obtain fine powder,
Add 6 times of 20% gelatinized corn starches of amount, 1 times of amount 10%PVP (PVP) ethanol, granulation, drying, compressing tablet.
6. a kind of extraction from Semen Cuscutae according to claim 3 prevents and treats hyperuricemia and gout medicine-food two-purpose monomer
Preparation method, it is characterised in that the preparation method of the mixture is：Gained eluent concentrates or is diluted to relative density 1.2
Medicinal extract, bottling makes oral liquid.
7. a kind of extraction from Semen Cuscutae according to claim 3 prevents and treats hyperuricemia and gout medicine-food two-purpose monomer
Preparation method, it is characterised in that the preparation method of the food additives is：Gained eluent be concentrated in vacuo, vacuum drying or
It is spray-dried to obtain fine powder, you can as food additives.
8. a kind of extraction from Semen Cuscutae according to claim 3 prevents and treats hyperuricemia and gout medicine-food two-purpose monomer
Preparation method, it is characterised in that the preparation method of the food additives is：The concentration of gained eluent is relative density 1.2
Medicinal extract, you can as food additives.
9. a kind of extraction from Semen Cuscutae according to claim 3 prevents and treats hyperuricemia and gout medicine-food two-purpose monomer
Preparation method, it is characterised in that the preparation method of the health protection tea is：Gained eluent is concentrated under reduced pressure into relative density 1.2
Medicinal extract, 1 is pressed with Tie Guanyin tea：25 mass ratio mixing, dries 8 hours under the conditions of being placed in 55 DEG C, then is ground into 20 mesh, sterilizes
Packaging and storage, is made the anti-trioxypurine health protection tea of the bags of 6g mono-.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107495378A (en) * | 2017-10-13 | 2017-12-22 | 武汉华康臣生物科技有限公司 | A kind of functional food suitable for gout and people with high uric acid |
| CN108721538A (en) * | 2017-08-23 | 2018-11-02 | 极原国际健康管理服务有限公司 | A kind of drink prevented or treat hyperuricemia and gout |
| CN114949069A (en) * | 2022-06-29 | 2022-08-30 | 劲牌有限公司 | Uric acid-reducing traditional Chinese medicine composition for inhibiting xanthine oxidase and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102895320A (en) * | 2012-04-16 | 2013-01-30 | 全金花 | Medicinal food formula and medicinal edible preparation for preventing and treating hyperuricemia and gout |
| KR20130136151A (en) * | 2012-06-04 | 2013-12-12 | 권태선 | Improving gout, reducing uric acid, solving hangover of functional fermented food and method for producing of the same, and kimchi, gochujang, doenjang, soy sauce produced thereof |
-
2016
- 2016-11-14 CN CN201610996958.XA patent/CN106727857A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102895320A (en) * | 2012-04-16 | 2013-01-30 | 全金花 | Medicinal food formula and medicinal edible preparation for preventing and treating hyperuricemia and gout |
| KR20130136151A (en) * | 2012-06-04 | 2013-12-12 | 권태선 | Improving gout, reducing uric acid, solving hangover of functional fermented food and method for producing of the same, and kimchi, gochujang, doenjang, soy sauce produced thereof |
Non-Patent Citations (6)
| Title |
|---|
| X. LING ET AL.: "A review of phytotherapy of gout:perspective of new pharmacological treatments", 《PHARMAZIE》 * |
| 山东省食品药品监督管理局: "《山东省中药饮片炮制规范》", 31 January 2013, 山东科学技术出版社 * |
| 张晶等: "《中药化学》", 30 April 2015, 中国农业大学出版社 * |
| 汪茂田等: "《天然有机化合物提取分离与结构鉴定》", 30 September 2004, 化学工业出版社 * |
| 范江华等: "痛风发生因素分析与预防对策", 《中国医学创新》 * |
| 郭澄等: "菟丝子的化学成分和药理活性研究", 《时珍国医国药》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108721538A (en) * | 2017-08-23 | 2018-11-02 | 极原国际健康管理服务有限公司 | A kind of drink prevented or treat hyperuricemia and gout |
| CN107495378A (en) * | 2017-10-13 | 2017-12-22 | 武汉华康臣生物科技有限公司 | A kind of functional food suitable for gout and people with high uric acid |
| CN114949069A (en) * | 2022-06-29 | 2022-08-30 | 劲牌有限公司 | Uric acid-reducing traditional Chinese medicine composition for inhibiting xanthine oxidase and preparation method thereof |
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