CN106727808A - A kind of extracting method of notoginsenoside - Google Patents
A kind of extracting method of notoginsenoside Download PDFInfo
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- CN106727808A CN106727808A CN201710003467.5A CN201710003467A CN106727808A CN 106727808 A CN106727808 A CN 106727808A CN 201710003467 A CN201710003467 A CN 201710003467A CN 106727808 A CN106727808 A CN 106727808A
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- solution
- notoginsenoside
- aqueous solution
- ethanol
- ginseng
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The invention discloses a kind of extraction preparation method of notoginsenoside, comprise the following steps：（1）Fresh pseudo-ginseng is taken, is cleaned, dried, air-dry or dry；（2）By pseudo-ginseng root slices, extracted with the ethanol solution heat of 20 99v%, extract solution reclaims ethanol, add 26 times of the aqueous solution to be diluted to without the floating of obvious oily matter, filtering obtains clear aqueous solution；（3）By D101 large pore resin absorption columns on the clear aqueous solution of step 2, then washed with the aqueous solution of pH=4 61 time, then eluted with the ethanol solution of 40 80v%；（4）Eluent is collected, ethanol is reclaimed, is concentrated under reduced pressure into dry, obtain white powder product.The present invention carries out section and extracts processing using fresh pseudo-ginseng, purification process is carried out with large pore resin absorption column after processing, processing procedure is simple and easy to apply, the solution extraction efficiency for early stage is high, the purification process effect in later stage is good, can sufficiently obtain the notoginsenoside finished product of high-quality.
Description
Technical field
The present invention relates to a kind of traditional Chinese medicine ingredients extracting method, more particularly to a kind of extracting method of notoginsenoside belongs to biography
The technique of extracting of natural medicinal ingredients in system Chinese medicine.
Background technology
Pseudo-ginseng, is panax araliaceae plantPanax notoginseng (Burk.)F.H.ChenDry root and rhizome.
Pseudo-ginseng be it is a kind of abound with cloud, expensive, river traditional Chinese medicine, also known as Study on Sedum aizoon L, sweet micro- acid is mild-natured, nontoxic, can Huoxue San "
The stasis of blood, hemostasis, swelling and pain relieving.
《Compendium of Materia Medica》：Styptic powder blood analgesic therapy, golden sword arrow wound, falls and flutters cane sore, blood and go out more than person, chews rotten painting, or for end is mixed
It, its blood is to stop.Also main hematemesis and epistaxis, lower blood bloody flux, metrorrhagia incessant menorrhea, postpartum extravesated blood not under, blood fortune blood bitterly, Akamu carbuncle
Swollen, tiger stings all diseases of snakebite.
The extraction of Effective Component of Chinese Medicine prepares the key of the application for being Chinese medicine modernization medical application technology combination, due to
Native chemical complicated component causes to become more difficult in extraction preparation process in Chinese medicine, if deeply can not sufficiently study
The characteristic of native chemical composition is analyzed, and extraction processing is carried out for corresponding characteristic, then be likely to extract preparation naturalization
A point failure is studied, and then causes the modernization application of traditional Chinese medicine to be restricted.
The native chemical composition of pseudo-ginseng contains saponin(e, flavones, sterol, volatile oil, amino acid, polysaccharide etc., and composition is more multiple
It is miscellaneous, existing extractive technique for these compositions extraction more have it is certain restricted, it is difficult to break through composition in extracting completely
Purifying problem.
The content of the invention
It is difficult present in extraction preparation in the prior art for notoginsenoside it is an object of the invention to overcome, there is provided
A kind of extracting method of notoginsenoside.So that prepared by a high proportion of extraction the such as notoginsenoside R and ginsenoside Rg1 and Rb1 so that
Arasaponin extract can have been given play to and the crude drug efficacy effect that drug effect is mutually matched in itself.
In order to realize foregoing invention purpose, the invention provides following technical scheme：
A kind of extraction preparation method of notoginsenoside, comprises the following steps：
（1）Fresh pseudo-ginseng is taken, is cleaned, dried, air-dry or dry.
（2）By pseudo-ginseng root slices, extracted with the ethanol solution heat of 20-99v%, extract solution reclaims ethanol, adds 2-6 times of water
Solution is diluted to without the floating of obvious oily matter, and filtering obtains clear aqueous solution.
（3）By D101 large pore resin absorption columns on the clear aqueous solution of step 2, then 1 is washed with the aqueous solution of pH=4-6
It is secondary, then eluted with the ethanol solution of 40-80v%.
（4）Eluent is collected, ethanol is reclaimed, is concentrated under reduced pressure into dry, obtain white powder product.
The present invention carries out section and extracts processing using fresh pseudo-ginseng, is carried out with large pore resin absorption column after processing pure
Change is processed, and processing procedure is simple and easy to apply, and the solution extraction efficiency for early stage is high, and the purification process effect in later stage is good, Neng Gouchong
The notoginsenoside finished product for obtaining high-quality for dividing.
Further, step 1, adds the alum of 0.1-0.4wt% to be washed when washing fresh pseudo-ginseng, in solution
Wash.
Further, step 2, when pseudo-ginseng root slices, is cut into the thin slice less than 12mm, the preferably thin slice of 3-8mm.
Further, step 2, is extracted using the ethanol solution of 30-80v%.
Further, step 2, hot extraction process is：Sanqi tablets alcohol solution dipping is flooded, 70-90 DEG C is heated to, protected
Hold 1-3 hours, repeat to extract 3-5 times.
Further, during extraction, in volatile matter condensing reflux to extraction solution.Or volatile matter is directly reclaimed carry
Take.
Further, step 2, filter opening aperture when filtering is 0.22 μm.
Further, the ratio of step 3, the resin column footpath and pillar height is 1:30-60.
Further, step 3, the aqueous solution of pH=4-6 is dilute hydrochloric acid solution.The consumption of solution is large pore resin absorption column
1-2 times of volume.
Further, step 4, temperature is no more than 85 DEG C in process concentrated under reduced pressure, preferably no more than 80 DEG C.
Compared with prior art, beneficial effects of the present invention：
1. pseudo-ginseng extracting method of the invention is cut into slices using fresh pseudo-ginseng and extracted so that the efficiency high that heat is extracted, energy consumption
It is low, with selectivity it is good the characteristics of.
2. notoginsenoside of the present invention can be widely used in prevention and treatment cardiovascular and cerebrovascular disease, old in improvement
Year people hypertension, high fat of blood have good effect.
Specific embodiment
With reference to test example and specific embodiment, the present invention is described in further detail.But this should not be understood
For the scope of above-mentioned theme of the invention is only limitted to following embodiment, all technologies realized based on present invention belong to this
The scope of invention.Not specified percentage should typically be weight percentage in the present invention, and solution proportion is not specified
Be percent by volume.
Embodiment 1
50 grams of fresh pseudo-ginseng is taken, the thin slice of 5-6mm is sliced into, adds 50v% alcohol solution dippings to flood, be heated to 88 DEG C of extractions,
Extract twice, merge and extract solution, add 3 times of pure water of volume, be diluted to solution surface without obvious oily floating object, on
D101 large pore resin absorption columns, are then washed 1 time with the dilute hydrochloric acid solution of pH=4.5, and the volume of dilute hydrochloric acid solution is equivalent to macropore
1.4 times of volumes of adsorption resin column, are then eluted with the ethanol solution of 80v%.Eluent is collected, ethanol is reclaimed, is concentrated under reduced pressure
To dry, white powder product is obtained.
Embodiment 2
50 grams of fresh pseudo-ginseng is taken, the thin slice of 7-8mm is sliced into, adds 60v% alcohol solution dippings to flood, be heated to 75 DEG C of extractions,
Extract twice, merge and extract solution, add 4 times of pure water of volume, be diluted to solution surface without obvious oily floating object, on
D101 large pore resin absorption columns, are then washed 1 time with the dilute hydrochloric acid solution of pH=6, and the volume of dilute hydrochloric acid solution is inhaled equivalent to macropore
2 times of volumes of attached resin column, are then eluted with the ethanol solution of 70v%.Eluent is collected, ethanol is reclaimed, is concentrated under reduced pressure into
It is dry, obtain white powder product.
Embodiment 3
Fresh pseudo-ginseng is taken, is cleaned with the alum solution containing 0.1wt%, 40-45 DEG C of hot-air seasoning.By pseudo-ginseng root slices, use
The ethanol solution heat of 65v% is extracted, and extract solution reclaims ethanol, adds 2 times of the aqueous solution to be diluted to without the floating of obvious oily matter,
0.22 μm of aperture strainer filtering, obtains clear aqueous solution.Upper D101 large pore resin absorption columns, are then washed with the aqueous solution of pH=5
1 time, then eluted with the ethanol solution of 70v%.Eluent is collected, ethanol is reclaimed, is concentrated under reduced pressure into dry, obtain white powder
Product.
Embodiment 4
Fresh pseudo-ginseng is taken, is cleaned with the alum solution containing 0.4wt%, clear water is rinsed once, is dried.By pseudo-ginseng root slices, use
The ethanol solution heat of 30v% is extracted, and extract solution reclaims ethanol, adds 3 times of the aqueous solution to be diluted to without the floating of obvious oily matter,
Filtering, obtains clear aqueous solution.Upper D101 large pore resin absorption columns, are then washed 1 time with the aqueous solution of pH=4, then use 40v%
Ethanol solution wash-out.Eluent is collected, ethanol is reclaimed, is concentrated under reduced pressure into dry, obtain white powder product.
Embodiment 5
Fresh pseudo-ginseng is taken, is cleaned with the alum solution containing 0.2wt%, clear water is rinsed once, 50 DEG C of hot-air seasonings.By three
Seven sections, are extracted with the ethanol solution heat of 35v%, and extract solution reclaims ethanol, add 2-6 times of the aqueous solution to be diluted to without substantially oil
Property material floats, filtering, obtain clear aqueous solution.Upper D101 large pore resin absorption columns, then wash 1 with the aqueous solution of pH=4-6
It is secondary, then eluted with the ethanol solution of 60v%.Collect eluent, reclaim ethanol, be concentrated under reduced pressure into it is dry, obtain white powder product
Thing.
Embodiment 6
Fresh pseudo-ginseng is taken, is cleaned with the alum solution containing 0.2wt%, clear water is rinsed once, is dried.By pseudo-ginseng root slices, use
The ethanol solution heat of 80v% is extracted, and extract solution reclaims ethanol, adds 6 times of the aqueous solution to be diluted to without the floating of obvious oily matter,
Filtering, obtains clear aqueous solution.Upper D101 large pore resin absorption columns, are then washed 1 time with the aqueous solution of pH=4, then use 80v%
Ethanol solution wash-out.Eluent is collected, ethanol is reclaimed, is concentrated under reduced pressure into dry, obtain white powder product.
Embodiment 7
Fresh pseudo-ginseng is taken, is cleaned, air-dried.By pseudo-ginseng root slices, extracted with the ethanol solution heat of 75v%, be heated to 75 DEG C, protected
Hold 2 hours, repeat to extract 3 times, merge extract solution, reclaim ethanol, add 5 times of the aqueous solution to be diluted to without the drift of obvious oily matter
Floating, filtering obtains clear aqueous solution.Upper D101 large pore resin absorption columns, then wash 1 time with the aqueous solution of pH=4-6, then
Eluted with the ethanol solution of 40-80v%.Collect eluent, reclaim ethanol, 75 DEG C be concentrated under reduced pressure into it is dry, obtain white powder product
Thing.
Claims (9)
1. a kind of extraction preparation method of notoginsenoside, comprises the following steps：
（1）Fresh pseudo-ginseng is taken, is cleaned, dried, air-dry or dry；
（2）By pseudo-ginseng root slices, extracted with the ethanol solution heat of 20-99v%, extract solution reclaims ethanol, adds 2-6 times of the aqueous solution
It is diluted to without the floating of obvious oily matter, filtering obtains clear aqueous solution；
（3）By D101 large pore resin absorption columns on the clear aqueous solution of step 2,1 time is then washed with the aqueous solution of pH=4-6, so
Eluted with the ethanol solution of 40-80v% afterwards；
（4）Eluent is collected, ethanol is reclaimed, is concentrated under reduced pressure into dry, obtain white powder product.
2. the extraction preparation method of notoginsenoside as claimed in claim 1, it is characterised in that step 1, washs fresh pseudo-ginseng
When, add the alum of 0.1-0.4wt% to be washed in solution.
3. the extraction preparation method of notoginsenoside as claimed in claim 1, it is characterised in that step 2, when pseudo-ginseng root slices,
It is cut into the thin slice less than 12mm.
4. the extraction preparation method of notoginsenoside as claimed in claim 1, it is characterised in that step 2, uses the second of 30-80v%
Alcoholic solution is extracted.
5. the extraction preparation method of notoginsenoside as claimed in claim 1, it is characterised in that step 2, hot extraction process is：Will
Sanqi tablets alcohol solution dipping floods, and is heated to 70-90 DEG C, is kept for 1-3 hours, repeats to extract 3-5 times.
6. the extraction preparation method of notoginsenoside as claimed in claim 1, it is characterised in that step 2, filter opening when filtering
Aperture is 0.22 μm.
7. the extraction preparation method of notoginsenoside as claimed in claim 1, it is characterised in that step 3, the resin column footpath and post
Ratio high is 1:30-60.
8. the extraction preparation method of notoginsenoside as claimed in claim 1, it is characterised in that step 3, the aqueous solution of pH=4-6 is
Dilute hydrochloric acid solution.
9. the extraction preparation method of notoginsenoside as claimed in claim 1, it is characterised in that step 4, in process concentrated under reduced pressure
Temperature is no more than 85 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710003467.5A CN106727808A (en) | 2017-01-04 | 2017-01-04 | A kind of extracting method of notoginsenoside |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710003467.5A CN106727808A (en) | 2017-01-04 | 2017-01-04 | A kind of extracting method of notoginsenoside |
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| CN106727808A true CN106727808A (en) | 2017-05-31 |
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| CN201710003467.5A Withdrawn CN106727808A (en) | 2017-01-04 | 2017-01-04 | A kind of extracting method of notoginsenoside |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107595908A (en) * | 2017-09-26 | 2018-01-19 | 云南金七制药有限公司 | A kind of extracting method that notoginsenoside is extracted from fresh pseudo-ginseng |
| CN113713002A (en) * | 2021-09-11 | 2021-11-30 | 深圳市真味生物科技有限公司 | Method for extracting Notoginseng radix extract and Notoginseng radix extract for atomized liquid |
-
2017
- 2017-01-04 CN CN201710003467.5A patent/CN106727808A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107595908A (en) * | 2017-09-26 | 2018-01-19 | 云南金七制药有限公司 | A kind of extracting method that notoginsenoside is extracted from fresh pseudo-ginseng |
| CN113713002A (en) * | 2021-09-11 | 2021-11-30 | 深圳市真味生物科技有限公司 | Method for extracting Notoginseng radix extract and Notoginseng radix extract for atomized liquid |
| CN113713002B (en) * | 2021-09-11 | 2023-10-27 | 深圳市真味生物科技有限公司 | Method for extracting pseudo-ginseng extract and extracted pseudo-ginseng extract for atomized liquid |
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Application publication date: 20170531 |