CN106727709B - Production process of sheep placenta extract - Google Patents
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- CN106727709B CN106727709B CN201611043823.8A CN201611043823A CN106727709B CN 106727709 B CN106727709 B CN 106727709B CN 201611043823 A CN201611043823 A CN 201611043823A CN 106727709 B CN106727709 B CN 106727709B
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- 210000002826 placenta Anatomy 0.000 title claims abstract description 86
- 241001494479 Pecora Species 0.000 title claims abstract description 81
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 51
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 238000001816 cooling Methods 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 21
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000006243 chemical reaction Methods 0.000 claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 16
- 238000002791 soaking Methods 0.000 claims abstract description 16
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 15
- 239000010935 stainless steel Substances 0.000 claims abstract description 15
- 235000013372 meat Nutrition 0.000 claims abstract description 9
- -1 amine sulfide Chemical class 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims abstract description 8
- 230000018044 dehydration Effects 0.000 claims abstract description 8
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 108010059712 Pronase Proteins 0.000 claims description 16
- 108090000631 Trypsin Proteins 0.000 claims description 16
- 102000004142 Trypsin Human genes 0.000 claims description 16
- 230000007935 neutral effect Effects 0.000 claims description 16
- 239000012588 trypsin Substances 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 239000012153 distilled water Substances 0.000 claims description 9
- 239000012535 impurity Substances 0.000 claims description 8
- 239000002202 Polyethylene glycol Substances 0.000 claims description 7
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 7
- 229920001223 polyethylene glycol Polymers 0.000 claims description 7
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 7
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 7
- 238000011049 filling Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 13
- 239000004094 surface-active agent Substances 0.000 abstract description 10
- 102000004190 Enzymes Human genes 0.000 abstract description 5
- 108090000790 Enzymes Proteins 0.000 abstract description 5
- 238000000227 grinding Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005485 electric heating Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102100037926 Divergent protein kinase domain 2A Human genes 0.000 description 1
- 101710081910 Divergent protein kinase domain 2A Proteins 0.000 description 1
- 102000019280 Pancreatic lipases Human genes 0.000 description 1
- 108050006759 Pancreatic lipases Proteins 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229940116369 pancreatic lipase Drugs 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Pregnancy & Childbirth (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Reproductive Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to the technical field of sheep placenta extract, in particular to a production process of sheep placenta extract. The method comprises the following production steps: s1, soaking fresh sheep placenta for 1-2 hours; s2, after soaking, cleaning the soaked materials by using clear water for 3-5 times, and then putting the materials into a centrifugal dehydrator for dehydration for 1-2 times; s3, putting the meat into a meat grinder for grinding; s4, carrying out two times of enzymolysis reaction under the premise of ultrasonic heating; s5, cooling the sterilized and enzyme-deactivated sheep placenta solution, standing at normal temperature, pouring into a stainless steel barrel, adding 2% of activated carbon, and heating for 3-5 min; s6, adding 3-5% of ethanol and 0.05-0.08% of amine sulfide, heating for 20-30 min, cooling to normal temperature, and filtering to obtain the sheep placenta extract. The production process is simple, the use amount of enzyme is reduced, and the enzymolysis efficiency is improved by using the ultrasonic waves and the surfactant in a matching manner, so that the extraction efficiency of the sheep placenta extracting solution is improved.
Description
Technical Field
The invention relates to the technical field of sheep placenta extract, in particular to a production process of sheep placenta extract.
Background
The placenta extract is a common health product, and is a micromolecular compound which is extracted from placenta and has immunocompetence, and is also called placenta peptide. The theory of traditional Chinese medicine holds that the placenta is dry and salty, and enters lung and kidney meridians. Has the functions of benefiting qi, nourishing blood, benefiting essence, nourishing, beautifying and strengthening. The efficacy mainly comprises the following points: has health promoting, immunity and motion ability improving, antifatigue, antiaging, and cellular immunity improving effects. Because the placenta has the above-mentioned functions, the extraction and separation of its effective components, i.e. polypeptide, SOD, ATP, HASF, EGF, etc., by modern biotechnology and modern chemical method is an important subject. The method of extracting sheep placenta by dialysis and ultrafiltration and processing the sheep placenta into high-tech products for clinical medicine has been reported as early as 80 years. The existing sheep placenta extract extracting process is complex, and the dosage of enzyme in the extracting process is large, so that the extracting cost is high. The extraction rate of the sheep placenta extract is about 50 percent generally, the utilization efficiency of sheep placenta is not high, and great resource waste is caused.
Disclosure of Invention
The invention aims at solving the technical problems that the production process of the sheep placenta extract is simple, reduces the water content of sheep placenta by pretreatment, further reduces the use amount of enzyme and reduces the extraction cost; the enzymolysis efficiency is improved by the matching use of ultrasonic waves and a surface active agent, so that the extraction efficiency of the whole sheep placenta extracting solution is improved.
In order to solve the technical problems, the invention provides a production process of a sheep placenta extract, which comprises the following production steps: s1, selecting fresh sheep placenta, removing impurities, and soaking in a stainless steel barrel for 1-2 hours; s2, after soaking, cleaning the soaked materials by using clear water for 3-5 times, and then putting the materials into a centrifugal dehydrator for dehydration for 1-2 times; s3, crushing the dried sheep placenta in a meat grinder; s4, putting the crushed sheep placenta into a reaction kettle, adding distilled water, adjusting the pH value to 8.0-8.5, putting the reaction kettle into an ultrasonic water bath heating pot, heating for 10-20 min, cooling, keeping the temperature to 40-50 ℃, adding 0.5% of trypsin and 1.2% of Tween-20, performing enzymolysis for 3-5 hours, and heating to 70-80 ℃ to kill the trypsin; cooling and preserving the temperature to 50-60 ℃, adjusting the pH to 7.0, adding neutral pronase and polyethylene glycol, carrying out enzymolysis for 5-8 hours, heating to 100-110 ℃ to kill the neutral pronase, and carrying out sterilization treatment; s5, cooling the sterilized and enzyme-deactivated sheep placenta solution, standing at normal temperature, pouring into a stainless steel barrel, adding 2% of activated carbon, and heating for 3-5 min; s6, adding 3-5% of ethanol and 0.05-0.08% of amine sulfide, heating for 20-30 min, cooling to normal temperature, filtering to obtain a sheep placenta extract, filling, sealing, and refrigerating in a refrigerator for later use.
Specifically, in the step S4, the ultrasonic water bath heating parameter is that the ultrasonic power is 30W, and the heating time is 18 min.
Specifically, the heating temperature of the ultrasonic water bath in the S4 is 80-90 ℃.
Further, in the above technical solution, the sterilization in S4 is performed at a high temperature, the sterilization temperature is 130 ℃ to 150 ℃, and the sterilization time is 3 to 5 seconds.
Further limiting, in the above technical solution, the feed-liquid ratio of the sheep placenta in S4 to the distilled water is 1: 1.5.
After the production process is adopted, the process is simple, the moisture content of the sheep placenta is reduced through pretreatment, the use amount of enzyme is further reduced, and the extraction cost is reduced; the enzymolysis efficiency is improved by the matching use of ultrasonic waves and a surface active agent, so that the extraction efficiency of the whole sheep placenta extracting solution is improved.
Detailed Description
In the invention, S1, selecting fresh sheep placenta, removing impurities, and soaking in a stainless steel barrel for 1-2 hours, so that the impurities adsorbed in the sheep placenta are diffused, and operators can clean the sheep placenta conveniently; s2, after soaking, cleaning the soaked materials by using clear water for 3-5 times, and then putting the materials into a centrifugal dehydrator for dehydration for 1-2 times; the water contained in the sheep placenta can be fully removed, because the water content of the sheep placenta reaches 70% -80% of the water content of the sheep placenta, if the water is not completely removed, the later enzymolysis process can be influenced, and the use amount of enzyme in the enzymolysis process can be increased. S3, crushing the dried sheep placenta in a meat grinder; in order to make the subsequent enzymolysis process more sufficient. S4, putting the crushed sheep placenta into a reaction kettle, adding distilled water, adjusting the pH value to 8.0-8.5, putting the reaction kettle into an ultrasonic water bath heating pot, heating for 10-20 min, cooling, keeping the temperature to 40-50 ℃, adding 0.5% of trypsin and 1.2% of Tween-20, performing enzymolysis for 3-5 hours, and heating to 70-80 ℃ to kill the trypsin; cooling and preserving the temperature to 50-60 ℃, adjusting the pH value to 7.0, adding neutral pronase and polyethylene glycol, carrying out enzymolysis for 5-8 hours, then heating to 100-110 ℃ to kill the neutral pronase, and carrying out sterilization treatment at the same time. The method adopts two times of enzymolysis, the first time of enzymolysis, 0.5 percent of trypsin and 1.2 percent of Tween-20, because the sheep placenta contains a small amount of fat, sugar and a large amount of protein, the trypsin can be adopted to ensure that pancreatic lipase and pancreatic amylase in the sheep placenta have certain decomposition effect, and the 1.2 percent of Tween-20 is a surfactant, so that the enzymolysis process can be promoted, and the extraction rate is improved; neutral pronase and polyethylene glycol are added in the second enzymolysis process, the neutral pronase can carry out a large amount of enzymolysis on protein in the sheep placenta, and the polyethylene glycol is a surfactant, so that the enzymolysis process can be effectively promoted, and the extraction rate of the extracting solution is improved; the whole process is completed in the ultrasonic water bath heating pot, so that the ultrasonic water bath heating greatly promotes the enzymolysis process and improves the extraction rate of the extracting solution; s5, cooling the sterilized and enzyme-deactivated sheep placenta solution, standing at normal temperature, pouring into a stainless steel barrel, adding 2% of activated carbon, and heating for 3-5 min; s6, adding 3-5% of ethanol and 0.05-0.08% of amine sulfide, heating for 20-30 min, cooling to normal temperature, filtering to obtain a sheep placenta extract, filling, sealing, and refrigerating in a refrigerator for later use to treat the smell and color of the sheep placenta extract, thereby ensuring that the sheep placenta extract is colorless and tasteless.
In order to verify that the production process of the present invention compares with the conventional production process, the inventors performed the following comparison of several sets of experiments:
the first embodiment is as follows:
the following production process is adopted: s1, selecting fresh sheep placenta, removing impurities, and soaking in a stainless steel barrel for 1-2 hours; s2, after soaking, cleaning the soaked materials by using clear water for 3-5 times, and then putting the materials into a centrifugal dehydrator for dehydration for 1-2 times; s3, crushing the dried sheep placenta in a meat grinder; s4, putting the crushed sheep placenta into a reaction kettle, adding distilled water, adjusting the pH value to 8.0-8.5, putting the reaction kettle into an electric heating constant-temperature water bath, heating for 10-20 min, cooling, keeping the temperature to 40-50 ℃, adding 0.5% of trypsin, performing enzymolysis for 3-5 hours, and heating to 70-80 ℃ to kill the trypsin; cooling and preserving the temperature to 50-60 ℃, adjusting the pH to 7.0, adding neutral pronase, carrying out enzymolysis for 5-8 hours, then heating to 100-110 ℃ to kill the neutral pronase, and carrying out sterilization treatment at the same time; s5, cooling the sterilized and enzyme-deactivated sheep placenta solution, standing at normal temperature, pouring into a stainless steel barrel, adding 2% of activated carbon, and heating for 3-5 min; s6, adding 3-5% of ethanol and 0.05-0.08% of amine sulfide, heating for 20-30 min, cooling to normal temperature, and filtering to obtain the sheep placenta extract A.
Example two:
the following production process is adopted: s1, selecting fresh sheep placenta, removing impurities, and soaking in a stainless steel barrel for 1-2 hours; s2, after soaking, cleaning the soaked materials by using clear water for 3-5 times, and then putting the materials into a centrifugal dehydrator for dehydration for 1-2 times; s3, crushing the dried sheep placenta in a meat grinder; s4, putting the crushed sheep placenta into a reaction kettle, adding distilled water, adjusting the pH value to 8.0-8.5, putting the reaction kettle into an ultrasonic water bath heating pot, heating for 10-20 min, cooling, keeping the temperature to 40-50 ℃, adding 0.5% of trypsin, performing enzymolysis for 3-5 hours, and heating to 70-80 ℃ to kill the trypsin; cooling and preserving the temperature to 50-60 ℃, adjusting the pH to 7.0, adding neutral pronase, carrying out enzymolysis for 5-8 hours, then heating to 100-110 ℃ to kill the neutral pronase, and carrying out sterilization treatment at the same time; s5, cooling the sterilized and enzyme-deactivated sheep placenta solution, standing at normal temperature, pouring into a stainless steel barrel, adding 2% of activated carbon, and heating for 3-5 min; s6, adding 3-5% of ethanol and 0.05-0.08% of amine sulfide, heating for 20-30 min, cooling to normal temperature, and filtering to obtain a sheep placenta extract B.
Example three:
the following production process is adopted: s1, selecting fresh sheep placenta, removing impurities, and soaking in a stainless steel barrel for 1-2 hours; s2, after soaking, cleaning the soaked materials by using clear water for 3-5 times, and then putting the materials into a centrifugal dehydrator for dehydration for 1-2 times; s3, crushing the dried sheep placenta in a meat grinder; s4, putting the crushed sheep placenta into a reaction kettle, adding distilled water, adjusting the pH value to 8.0-8.5, putting the reaction kettle into an electric heating constant-temperature water bath, heating for 10-20 min, cooling, keeping the temperature to 40-50 ℃, adding 0.5% of trypsin and 1.2% of Tween-20, performing enzymolysis for 3-5 hours, and heating to 70-80 ℃ to kill the trypsin; cooling and preserving the temperature to 50-60 ℃, adjusting the pH to 7.0, adding neutral pronase and polyethylene glycol, carrying out enzymolysis for 5-8 hours, heating to 100-110 ℃ to kill the neutral pronase, and carrying out sterilization treatment; s5, cooling the sterilized and enzyme-deactivated sheep placenta solution, standing at normal temperature, pouring into a stainless steel barrel, adding 2% of activated carbon, and heating for 3-5 min; s6, adding 3-5% of ethanol and 0.05-0.08% of amine sulfide, heating for 20-30 min, cooling to normal temperature, and filtering to obtain a sheep placenta extract C.
Example four:
the following production process is adopted: the following production process is adopted: s1, selecting fresh sheep placenta, removing impurities, and soaking in a stainless steel barrel for 1-2 hours; s2, after soaking, cleaning the soaked materials by using clear water for 3-5 times, and then putting the materials into a centrifugal dehydrator for dehydration for 1-2 times; s3, crushing the dried sheep placenta in a meat grinder; s4, putting the crushed sheep placenta into a reaction kettle, adding distilled water, adjusting the pH to 8.0-8.5, heating the reaction kettle in an ultrasonic water bath at the ultrasonic power of 30W for 18min at the ultrasonic water bath heating temperature of 80-90 ℃, cooling and preserving the temperature to 40-50 ℃, adding 0.5% of trypsin and 1.2% of Tween-20, carrying out enzymolysis for 3-5 hours, and heating to 70-80 ℃ to kill the trypsin; cooling and preserving the temperature to 50-60 ℃, adjusting the pH to 7.0, adding neutral pronase and polyethylene glycol, carrying out enzymolysis for 5-8 hours, heating to 100-110 ℃ to kill the neutral pronase, and carrying out sterilization treatment; s5, cooling the sterilized and enzyme-deactivated sheep placenta solution, standing at normal temperature, pouring into a stainless steel barrel, adding 2% of activated carbon, and heating for 3-5 min; s6, adding 3-5% of ethanol and 0.05-0.08% of amine sulfide, heating for 20-30 min, cooling to normal temperature, and filtering to obtain a sheep placenta extract D.
The embodiment is that the sheep placenta extract A is not heated by ultrasonic waves and extracted by a surfactant; the second embodiment adopts ultrasonic wave to heat sheep placenta extract B without using a surface active agent; in the third embodiment, a sheep placenta extract C extracted by a surfactant without ultrasonic heating is adopted; in the fourth embodiment, the sheep placenta extract D is extracted by ultrasonic heating and a surfactant at the same time. The sheep placenta extract A, B, C, D has extraction rates of 45.2%, 52.8%, 58.6% and 68.8% respectively, calculated by nitrogen content.
According to the data, the extraction rate of the sheep placenta extract can be improved by 23.6% by simultaneously adopting ultrasonic heating and a surfactant to extract the sheep placenta extract, the extraction rate is greatly improved, the effective utilization of the sheep placenta is promoted, and the waste of sheep placenta resources is reduced.
Although specific embodiments of the present invention have been described above, it will be appreciated by those skilled in the art that these are merely examples and that many variations or modifications may be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims.
Claims (4)
1. The production process of the sheep placenta extract is characterized by comprising the following production steps:
s1, selecting fresh sheep placenta, removing impurities, and soaking in a stainless steel barrel for 1-2 hours;
s2, after soaking, cleaning the soaked materials by using clear water for 3-5 times, and then putting the materials into a centrifugal dehydrator for dehydration for 1-2 times;
s3, crushing the dried sheep placenta in a meat grinder;
s4, putting the crushed sheep placenta into a reaction kettle, adding distilled water, adjusting the pH value to 8.0-8.5, putting the reaction kettle into an ultrasonic water bath, heating the reaction kettle for 18min under the ultrasonic power of 30W, cooling and preserving the temperature to 40-50 ℃, adding 0.5% of trypsin and 1.2% of Tween-20, performing enzymolysis for 3-5 hours, and heating to 70-80 ℃ to kill the trypsin; cooling and preserving the temperature to 50-60 ℃, adjusting the pH to 7.0, adding neutral pronase and polyethylene glycol, carrying out enzymolysis for 5-8 hours, heating to 100-110 ℃ to kill the neutral pronase, and carrying out sterilization treatment;
s5, cooling the sterilized and enzyme-deactivated sheep placenta solution, standing at normal temperature, pouring into a stainless steel barrel, adding 2% of activated carbon, and heating for 3-5 min;
s6, adding 3-5% of ethanol and 0.05-0.08% of amine sulfide, heating for 20-30 min, cooling to normal temperature, filtering to obtain a sheep placenta extract, filling, sealing, and refrigerating in a refrigerator for later use.
2. The process for producing the sheep placenta extract as claimed in claim 1, wherein the process comprises the following steps: and the heating temperature of the ultrasonic water bath in the S4 is 80-90 ℃.
3. The process for producing the sheep placenta extract as claimed in claim 1, wherein the process comprises the following steps: and the sterilization treatment in the S4 is high-temperature sterilization treatment, the sterilization temperature is 130-150 ℃, and the sterilization time is 3-5S.
4. The process for producing the sheep placenta extract as claimed in claim 1, wherein the process comprises the following steps: the feed-liquid ratio of the sheep placenta to the distilled water in the S4 is 1: 1.5.
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| CN108685828A (en) * | 2018-06-14 | 2018-10-23 | 成都医学院 | A kind of extracting method of placental hormone |
| CN108866136A (en) * | 2018-08-01 | 2018-11-23 | 李文锦 | A kind of preparation method of low molecular polypeptide |
| CN110613128A (en) * | 2019-07-24 | 2019-12-27 | 宁波华子制药设备制造有限公司 | Low-temperature extraction production process of active sheep placenta |
| CN111820404A (en) * | 2020-07-07 | 2020-10-27 | 肖启森 | Sheep embryo extract beautifying oral liquid and preparation method thereof |
| CN114766619A (en) * | 2022-05-30 | 2022-07-22 | 中山市美太保健制品有限公司 | Health-care product oral liquid for delaying senility and preparation method thereof |
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| CN1481725A (en) * | 2003-07-16 | 2004-03-17 | 杨国春 | Method for preparing goat fetal hormone products |
| CN102318816A (en) * | 2011-05-19 | 2012-01-18 | 大连九羊食品有限公司 | Sheep placenta extraction process |
| CN102327290A (en) * | 2011-07-12 | 2012-01-25 | 湖州康海斯生物科技有限公司 | Refined sheep placenta extract prepared from sheep embryo and sheep placental peptide and preparation method |
| CN103181553A (en) * | 2011-12-30 | 2013-07-03 | 广州拜迪生物医药有限公司 | Pig placentin production method |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1481725A (en) * | 2003-07-16 | 2004-03-17 | 杨国春 | Method for preparing goat fetal hormone products |
| CN102318816A (en) * | 2011-05-19 | 2012-01-18 | 大连九羊食品有限公司 | Sheep placenta extraction process |
| CN102327290A (en) * | 2011-07-12 | 2012-01-25 | 湖州康海斯生物科技有限公司 | Refined sheep placenta extract prepared from sheep embryo and sheep placental peptide and preparation method |
| CN103181553A (en) * | 2011-12-30 | 2013-07-03 | 广州拜迪生物医药有限公司 | Pig placentin production method |
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