CN106725982A - A kind of mouse blood-taking device - Google Patents
A kind of mouse blood-taking device Download PDFInfo
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- CN106725982A CN106725982A CN201710040013.5A CN201710040013A CN106725982A CN 106725982 A CN106725982 A CN 106725982A CN 201710040013 A CN201710040013 A CN 201710040013A CN 106725982 A CN106725982 A CN 106725982A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/15003—Source of blood for venous or arterial blood
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150343—Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
- A61B5/154—Devices using pre-evacuated means
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
- A61D2003/006—Appliances for supporting or fettering animals for operative purposes with leg or foot restraining means
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Abstract
The invention discloses a kind of mouse blood-taking device, the rapid undertaking of shortage between docking and collection two steps of blood in existing mouse blood collection procedure is solved, so as to cause afterbody blood coagulation to cause the problem of blood sampling deficiency.The blood-taking device includes mouse limit assembly, docking device, and blood collection unit, wherein, mouse limit assembly, the four limbs for fixing mouse；Docking device, for cutting off Mouse Tail-tip；Blood collection unit, for providing vacuum environment, and contains the blood of mouse docking surface of a wound drippage.
Description
Technical field
The present invention relates to mouse experiment technical field, and in particular to a kind of mouse blood-taking device.
Background technology
Due to mouse gene order and the mankind it is similar, therefore, the scientific research and clinical trial of some medical science be all by
Mouse completes.In whole experiment process, generally require repeatedly to take a blood sample mouse.Now the more commonly used blood sampling side
Method is docking blood collection method, on the one hand, easy to operate；On the other hand, it is ensured that mouse still survives after blood sampling.
Docking blood collection method, generally includes four steps：Fixed mouse, heating afterbody (illumination or hot bath), docking and receipts
Collection blood, in actual mechanical process, in order to increase blood sampling volume, while do not influence nature blood coagulation (avoiding mouse excessive blood loss) again,
Often after mouse is docked, putting it into a negative pressure of vacuum environment carries out blood collection.So, due to docking and collecting blood
Existence time is poor between liquid the two steps, and coagulation process has occurred and that from that time of docking, therefore, cause vacuum to be born
The environment of pressure does not play one's part to the full.
The content of the invention
In view of this, a kind of mouse blood-taking device is the embodiment of the invention provides, in solving existing mouse blood collection procedure
Lack rapid undertaking between docking and collection two steps of blood, so as to cause afterbody blood coagulation to cause the problem of blood sampling deficiency.
A kind of mouse blood-taking device that one embodiment of the invention is provided, including mouse limit assembly, docking device, and blood
Collector, wherein,
Mouse limit assembly, the four limbs for fixing mouse；
Docking device, for cutting off Mouse Tail-tip；
Blood collection unit, for providing vacuum environment, and contains the blood of mouse docking surface of a wound drippage.
A kind of mouse docking blood-taking device provided in an embodiment of the present invention, by mouse limit assembly, docking device, and blood
Collector is integrated, and docking and collection two steps of blood is closely connected, and shortens interval time, reduces blood coagulation, to the greatest extent may be used
Can increased blood sampling volume.
Brief description of the drawings
Fig. 1 show a kind of structural representation of mouse blood-taking device of one embodiment of the invention offer.
Fig. 2 show the dimensional structure diagram of the position-limit foot tweezer 53 of one embodiment of the invention offer.
Upper and lower two width figure shown in Fig. 3 is respectively the position-limit foot tweezer 53 as shown in Figure 2 of one embodiment of the invention offer
Internal structure top view and the schematic cross-section along A-A1 lines.
Fig. 4 show the dimensional structure diagram of the docking device of one embodiment of the invention offer.
Fig. 5 show Fig. 1 includes the partial structurtes internal structure schematic diagram of docking device shown in Fig. 4.
Fig. 6 show the operating circuit structured flowchart of the mouse blood-taking device of one embodiment of the invention offer.
Fig. 7 show the internal structure schematic diagram of mouse blood-taking device shown in Fig. 1.
In figure：
2 --- temperature controller；3 --- time controller；5 --- counter；8 --- pressure vacuum gauge；9 --- deflate
Valve；10 --- gas outlet；11 --- air pump；12 --- air inlet；13 --- contact-making switch；14 --- sliding bar；15——
Sliding groove；16 --- bourdon tube；17 --- air branch pipe；18 --- valve body；20 --- second support bar；21 --- magnet ring；
22 --- round iron piece；23 --- centrifuge shield；24 --- centrifuge tube；25 --- air intake；26 --- blood collection unit；
27 --- card；28 --- convex lens；29 --- tail point position-arresting disk；30 --- spoke shape blade；31 --- not cutting blade；32——
Screw through hole；33 --- driven gear；34 --- top cover；35 --- pipe；36 --- docking device；37 --- driving gear；
38 --- stepper motor；39 --- controllor for step-by-step motor；40 --- hot-blast outlet；41 --- flat board；42 --- closed slide；
43 --- pin tweezer not moved end；44 --- pin tweezer mobile terminal；45 --- spring fixed seat；46 --- compression spring；47 --- push pedal；
48 --- clamp；49 --- draw-in groove；50 --- rubber blanket；51 --- extension spring；52 --- magnet piece；53 --- position-limit foot
Tweezer；54 --- heating wire；56 --- first support bar.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on this
Embodiment in invention, the every other reality that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example is applied, the scope of protection of the invention is belonged to.
Fig. 1 show a kind of dimensional structure diagram of mouse blood-taking device of one embodiment of the invention offer.From figure
As can be seen that the mouse blood-taking device includes, mouse limit assembly, docking device, and blood collection unit.Wherein,
Mouse limit assembly, the four limbs for fixing mouse.
Docking device, for cutting off Mouse Tail-tip.
Blood collection unit, for providing vacuum environment, and contains the blood of mouse docking surface of a wound drippage.
A kind of mouse docking blood-taking device provided in an embodiment of the present invention, by mouse limit assembly, docking device, and blood
Collector is integrated, and docking and collection two steps of blood is closely connected, and shortens interval time, reduces blood coagulation, to the greatest extent may be used
Can increased blood sampling volume.
In one embodiment, mouse limit assembly includes flat board 41 and the position-limit foot tweezer 53 being arranged on flat board, limit
Position pin tweezer 53 can be fixed on a range of optional position on flat board.The benefit for so setting is can be adjusted in order to position
It is whole, so as to adapt to mouse not of uniform size.In one embodiment, flat board 41 can use stainless steel, or other any metals
Material, the lower surface of position-limit foot tweezer 53 includes magnet piece 52.
Fig. 2 show the dimensional structure diagram of the position-limit foot tweezer 53 of one embodiment of the invention offer.Can from figure
Go out, position-limit foot tweezer 53 includes pin tweezer fixing end 43 and pin tweezer mobile terminal 44, pin tweezer fixing end 43 and pin tweezer mobile terminal 44 it is relative
Side indent each other, pin tweezer mobile terminal 44 can do translational motion with respect to pin tweezer fixing end 43.
In one embodiment, the indent position of pin tweezer mobile terminal 44 and pin tweezer fixing end 43 is placed with rubber blanket 50.This
Sample, can slow down pain when mouse is fastened, while increasing physical friction to avoid position-limit foot tweezer 53 sliding from mouse four limbs
Fall.
In one embodiment, position-limit foot tweezer 53 as shown in Figure 2 further includes control unit, and control unit includes solid successively
The shifting component of fixed connection, translation component, and limit assembly, wherein：
Shifting component, for triggering the translational motion of translation component, and determines translation direction.
Translation component, for driving pin tweezer mobile terminal to do translational motion.
Limit assembly, the position for limiting translation component.
The internal structure that upper and lower two width figure shown in Fig. 3 is respectively the position-limit foot tweezer 53 of one embodiment of the invention offer is bowed
View and the schematic cross-section along A-A1 lines.It can be seen that
Position-limit foot tweezer 53 is embodied as rectangular box structure, one group of rectangle of opposite flank of insertion is offered in rectangular box and is led to
Hole, the rectangular through-hole is internally provided with two rectangular flats for extending and being arranged parallel to along rectangular through-hole direction, this two
The opposite face of rectangular flat is recessed Baltimore groove respectively, and the section structure of the Baltimore groove is semicircle.One of rectangle
Flat board is fixed on a side of rectangular through-hole, referred to as pin tweezer fixing end 43, and another rectangular flat can be in rectangular through-hole
Do the translational motion away from or near pin tweezer fixing end 43, referred to as pin tweezer mobile terminal 44.
Control unit includes push pedal 47, closed slide 42, and V-type fork component, closed slide 42 and pin tweezer fixing end 43 and
Pin tweezer mobile terminal 44 is vertically set on pin tweezer mobile terminal 44 and pin tweezer fixing end 43, and push pedal 47 is with V-type fork component by circle
Cylindrical connector is fixedly connected with, and the two ends of cylinder-shaped link are overlapped on closed slide 42 respectively, and the two of cylinder-shaped link
A fixed sliding block is distinguished in the position that end contacts with closed slide 42, and the middle part of cylinder-shaped link passes through a compression spring 46
With the elastic connection of spring fixed seat 45 of the middle position on pin tweezer not moved end 43, V-type fork component includes that one is long and the other is short two
Individual branch, branch long implements into tabular clamp 48, the draw-in groove 49 on clamp 48 and the magnet piece 52 of rectangular box lower surface
Matching；Short branch implements into inverted frustro-pyramidal, and end is by extension spring 51 and the elastic connection of pin tweezer mobile terminal 51.
Mouse limit assembly according to present embodiment, compression spring 46 can be in close contact clamp 48 and draw-in groove 49 so as to
Block, with push pedal 47 be connected for pin tweezer mobile terminal 44 by extension spring 51, it is ensured that pin tweezer mobile terminal 44 also can be same when push pedal 47 is moved
Direction translates, so as to change pin tweezer distance not between moved end 43 and pin tweezer mobile terminal 44 to realize position-limit foot tweezer 53 to mouse four limbs
Fastening and fastening releasing.
Fig. 4 show the dimensional structure diagram of the docking device of one embodiment of the invention offer.It can be seen that disconnected
Cercoids includes axis, rotating part, and not cutting blade, and rotating part can be around middle shaft rotation, and cutting blade is not relatively fixed with axis, rotation
There are at least two through holes of size interval arrangement on transfer part along being circular layout, big through hole internal diameter is more than mouse tail end
External diameter, less than the external diameter of mouse tail end, the blade of cutting blade is not tangent with through hole for small through hole internal diameter.
Docking device according to present embodiment, in advance by through hole small on the tail point insertion rotating part of mouse, rotates
Rotating part, completes cutting, while mouse docking is stayed in small through hole shifts to side with the rotation of docking device, small is passed through with this
The adjacent big through hole of through hole is gone to immediately below the mouse docking surface of a wound, for the blood of drippage provides passage.
In one embodiment, rotating part is disc structure, including the upper and lower spoke shape blade 30 and tail point position-arresting disk for stacking
29, at least two through holes of size interval arrangement are arranged on tail point position-arresting disk 29, are provided with this extremely on spoke shape blade 30
Few two through holes are corresponded and insertion tool bore coaxial one by one, and the inner edge of insertion tool bore has blade, and internal diameter is more than small
The external diameter of mouse tail end.
In one embodiment, the quarter of each insertion tool bore position of correspondence is provided with four the week side of boss walls of spoke shape blade 30
The surrounding outer of degree line and/or tail point position-arresting disk 29 is provided with the graduation mark of each insertion hole site of correspondence.So, a side
Face, the insertion tool bore heart coaxial with the through hole on tail point position-arresting disk 29 when being easy to assemble on spoke shape blade 30；On the other hand, may be used
The angle for turning over is needed to provide reference with rotating part during docking for once mouse.
Describe the composition structure of docking device in detail below by a specific embodiment.
Fig. 5 show the partial structurtes internal structure schematic diagram of the docking device of one embodiment of the invention offer.With reference to Fig. 4 and
Fig. 5 can be seen that the docking device 36 to be included successively from top to bottom：Top cover 34, not driven gear 33, cutting blade 31, spoke shape blade
30th, tail point position-arresting disk 29, and card 27.Wherein,
The upper surface of top cover 34 is circle, and its upper surface offers the pipe 35 of insertion top cover 34 from top to bottom, the pipe
Internal diameter is more than the external diameter of mouse tail, and lower center position sets fluted (not shown).
Driven gear 33 is cyclic structure, and the lower edge of its four the week side of boss wall is provided with annular protrusion.
Cutting blade 31 is not sector structure, and blade is arranged on a radius of sector structure, and its center of circle Angle Position is provided with
Four-prism shape screw through hole 32.
Spoke shape blade 30 is disc structure, and its home position is provided with cylindrical screw through hole (not shown), edge
The circumferential direction of a concentric circles is provided with least one circular insertion tool bore on disk, and each circular insertion tool bore inner edge is deposited
In blade, the external diameter of the internal diameter more than mouse tail end of the circular insertion tool bore, the upper and lower side of the week side of boss wall of spoke shape blade 30 4
Edge is respectively arranged with annular protrusion up and down.
Tail point position-arresting disk 29 is disc structure, and the top edge of its four the week side of boss wall is provided with annular protrusion, and home position is set
There is cylindrical screw through hole (not shown), a concentric circles circumferential direction is provided with least two circles and passes through along disk
Through hole, at least two circular through hole is intervally arranged by small one and large one (the large and small finger tool bore internal diameter), wherein, big circle
The internal diameter of through hole provides passage more than the external diameter of mouse tail end for the blood for mouse docking surface of a wound drippage；Small
The internal diameter of circular through hole less than mouse tail end external diameter, it is spacing for being carried out to Mouse Tail-tip, and contain in vitro small
Rat-tail point.
Card 27 includes discoid supporting part, and supporting part outer extends downwardly out at least two claws, on supporting part
Home position is upwardly formed cylindrical bump, and cylindrical bump top is provided with screw thread.
A circle insertion tool bore on the not knife edge of cutting blade 31 and spoke shape blade 30 is ensured is tangent, and spoke
On the premise of the circular through hole center of circle on circular insertion tool bore and tail point position-arresting disk 29 on shape blade 30 is aligned, by card 27
On cylindrical bump sequentially pass through tail point position-arresting disk 29, spoke shape blade 30, not cutting blade 31, and driven tooth from top to bottom
Take turns the screw through hole 32 of 33 4 home positions.Wherein, four-prism shape screw through hole 32 not on cutting blade 31 and card
Cylindrical bump on piece 27 is mutually fitted together to so that cutting blade 31 and card 27 are not relatively fixed；The upward ring of spoke shape blade 30
Shape is raised mutually chimeric with the annular protrusion on driven gear 33, the ring on the downward raised and tail point position-arresting disk 29 of spoke shape blade 30
Shape is raised mutually chimeric so that spoke shape blade 30, driven gear 33, the three of tail point position-arresting disk 29 are relatively fixed, and can pass through
Cylindrical bump synchronous rotary of the cylindrical screw through hole of respective home position on card 27；Afterwards cylindric convex
The top risen screws down fixation.Finally, the pipe 35 on top cover 34 is ensured and the circular insertion knife on spoke shape blade 30
On the premise of the center of circle of the circular through hole on hole and tail point position-arresting disk 29 is aligned, top cover 34 is snapped onto into the cylinder on card 27
The raised top of shape, is fixed by the matching grooves of the lower section of top cover 34.
Docking device can be dismantled according to above-mentioned opposite process, so as to realize changing spoke shape blade 30 or not moving knife
Piece 31.
It will be understood by those skilled in the art that the upper surface of card 27 can not include cylindrical protrusions, but use and fix
Screw replaces the cylindrical protrusions, each part for assembling docking device.
Docking device according to embodiment of the present invention, imports Mouse Tail-tip, through spoke shape by the pipe 35 on top cover 34
A circular insertion tool bore on blade 30, in the small internal diameter circle through hole stayed on tail point position-arresting disk 29.Rotate from
Moving gear 33 is aligned up to the center of circle of the circular insertion tool bore adjacent with initial circle insertion tool bore of the pipe 35 on top cover 34, the phase
Between drive in contrast fixed spoke shape blade 30 and tail point position-arresting disk 29, cutting blade 31 does not cut to Mouse Tail-tip, cuts
In the small internal diameter circle through hole that the tail point got off is stayed on tail point position-arresting disk 29, in rotation process, the tail point for scaling off is with this
Small internal diameter circle through hole is translated to side, and the large diameter circle through hole adjacent with the small internal diameter circle through hole moves on to mouse
Immediately below the docking surface of a wound, from the blood of mouse docking surface of a wound outflow just sequentially pass through circular insertion tool bore on spoke shape blade 30,
Large diameter circle through hole on tail point position-arresting disk 29, is finally dropped in centrifuge tube 24 (be detailed description below).Tail point is spacing
Spacing very little, clipping time foot between the adjacent circular insertion tool bore on adjacent circular through hole and spoke shape blade 30 on disk 29
It is enough short, on the one hand, to avoid the blood of drippage from sticking on component, cause to waste and pollute；On the other hand, it is to avoid blood coagulation occurs, subtracts
The blood volume of few collection；Secondly, mouse need not be captured between docking and blood collection two steps, it is to avoid mouse is come off dirt by hair
Stain source.
Refering to Fig. 1 and Fig. 5, it can be seen that blood collection unit 26 includes cylinder shell, inside is vacuum, is broken
Cercoids 36 is overlapped on the top of blood collection unit 26 by driven gear 33, and card 27 stretches into inside blood collection unit 26 and solid
It is fixed, just to being disposed with centrifuge tube 24 under the pipe 35 on top cover 34 inside blood collection unit 26, for containing blood.It is this
Structure so that blood collection unit 26 is completely cut off local blood-collecting environment and mouse by debris such as hairs.
In one embodiment, blood collection unit 26 is transparent configuration.So can in order to observe the blood volume of collection, while
The graduation mark on observation docking device 36 can also be easy to.In one embodiment, the correspondence spoke shape on the side wall of blood collection unit 26
Convex lens 28 are set at the position of the graduation mark on blade 30 and/or the surrounding outer of tail point position-arresting disk 29.For entering to graduation mark
Row amplifies, and improves alignment precision.
In one embodiment, blood collection unit 26 further includes centrifuge shield 23, is fixed on inside cylinder shell,
For containing centrifuge tube 24.
According to the mouse docking blood-taking device that embodiment of the present invention is provided, by mouse limit assembly, docking device 36, blood
Collector 26 is integrated, and is to be capable of achieving docking operation, mouse tail after docking by the certain angle of rotary drive gear 33
The blood of surface of a wound drippage falls directly into centrifuge tube 24 and collects, it is to avoid docking and lack rapid undertaking between collecting two steps of blood
Deficiency, weaken nature blood coagulation to docking blood sampling adverse effect；A kind of relatively independent blood-collecting environment is built simultaneously, has been kept away
Exempt from the pollution of blood source.
In one embodiment, mouse blood-taking device as shown in Figure 1 further includes to be coupled with driven gear 33
Driving gear 37 and operating circuit, operating circuit are used to drive driving gear to rotate.The root that Fig. 6 is provided for one embodiment of the invention
According to the structured flowchart of the operating circuit of the mouse docking device of embodiment of the present invention.The work is can be seen that with reference to Fig. 1 and Fig. 6
Circuit includes power switch 6, SMD temperature sensor, contact-making switch 13, time controller 3, controllor for step-by-step motor 39, step
Stepper motor, and counter 5.Wherein, SMD temperature sensor is pasted onto inside the pipe 35 on top cover 34, when SMD temperature
The temperature value that degree sensor is detected (has inserted the mouse tail for heating when being more than or equal to given threshold inside pipe 35
Portion), triggering contact-making switch 13 is turned on, and starts time controller 3.Time controller 3 connected by normally opened relay circuit and
Control controllor for step-by-step motor 39, when the timing of time controller 3 starts, controllor for step-by-step motor 39 is started and to meter by control
Number devices 5 send the period of work, drive driven gear 37 and the tail point position-arresting disk 29, the spoke shape blade 30 and driven that are linked with it
Gear 33 turns an angle, and realizes the shearing to mouse tail.The setting of operating circuit can realize automatic docking, save people
Work cost.
Fig. 7 show the internal structure schematic diagram of mouse blood-taking device shown in Fig. 1.
In one embodiment, mouse blood-taking device as shown in Figure 1 and Figure 7 further includes, negative pressure of vacuum assisted group
Part, the negative pressure of vacuum accessory part includes air pump 11, pressure vacuum gauge 8, vent valve 9.Wherein, air pump 11 includes gas outlet
10 and air inlet 12, the connection of gas outlet 10 extraneous air, the connection blood collection unit 26 of air inlet 12, pressure vacuum gauge 8 is contained within
The bourdon tube 16 of pressure is detected, the valve body 18 and bourdon tube 16 of vent valve 9 are by air branch pipe 17 and the air inlet of air pump 11
12 are connected.The gas in blood collection unit 26 is extracted out when air pump 11 works, is discharged into air by gas outlet 10, make blood
It is changed into negative pressure state inside collection 26, the size of negative pressure is shown by pressure vacuum gauge 8 and adjusted by vent valve 9.
In one embodiment, it is engaged with the negative pressure of vacuum accessory part, in the internal cavities of blood collection unit 26
Open tubular column is provided with, there is the air intake 25 of covering gauze its closed, side in top, and bottom connects with the air inlet 12 of air pump 11
It is logical.This way it is possible to avoid impurity (such as the mouse for coming off is by hair) the air inlet pump 11 inside blood collection unit 26.
In this case, the operating circuit of mouse blood-taking device further includes, air pump.Refering to Fig. 6, SMD temperature
Degree sensor is pasted onto inside the pipe 35 on top cover 34, when the temperature value that SMD temperature sensor is detected is more than or equal to
(mouse tail for heating is inserted inside pipe 35) during given threshold, triggering contact-making switch 13 is turned on, started time control
Device processed 3.In this case, the timing of time controller 3 can be set and have two-way to export, and lead up to normally closed relay circuit
Connection and control air pump 11, separately lead up to normally opened relay circuit connection and control controllor for step-by-step motor 39.Time
When the timing of controller 3 starts, controllor for step-by-step motor 39 is started by control and the period of work, band is sent to counter 5
Dynamic driving gear 37 and its tail point position-arresting disk 29 of linkage, spoke shape blade 30 and driven gear 33 turn an angle, and it is right to realize
Mouse tail is sheared, and air pump 11 is stopped after timing terminates.
Mouse blood-taking device according to present embodiment, it is not necessary to extra vacuum extractor, but be built in whole
Within individual device, integration is realized.
In one embodiment, mouse blood-taking device as shown in Figure 1 and Figure 7 can further include, Tail-heating
Component, is arranged on the gas outlet 10 of air pump 11, and hot-blast outlet 40 is in the horizontal direction just to the upper surface of top cover 34.At one
In embodiment, the heating component is heating wire.
In this case, the operating circuit of mouse blood-taking device further includes heating wire 54, refering to Fig. 6, SMD temperature
Degree sensor is pasted onto inside the pipe 35 on top cover 34, and the temperature threshold of temperature controller 2 can be set and have two-way to export, and one
Road is connected and control time controller 3 by normally opened relay circuit, separately lead up to normally closed relay circuit connection and
Control heating wire 54.When the temperature that (has mouse tail in pipe) in pipe 35 is less than temperature threshold, temperature controller 2 starts electricity
Heated filament 54, heats to the air that air pump 11 is pumped out, and when temperature reaches temperature threshold in pipe, heating wire 54 stops work
Make, meanwhile, start the timing control of time controller 3.
The timing of time controller 3 can be set and have two-way to export, and lead up to normally closed relay circuit connection and control
Air pump processed 11, separately leads up to normally opened relay circuit connection and control controllor for step-by-step motor 39.Time controller 3
When timing starts, controllor for step-by-step motor 39 is started by control and the period of work is sent to counter 5, drives stepper motor
38 and its tail point position-arresting disk 29 of linkage, spoke shape blade 30 and driven gear 33 turn an angle, realization is cut to mouse tail
Cut, air pump 11 is stopped after timing terminates.
Mouse blood-taking device according to present embodiment, will directly vacuumize the waste gas heating of discharge, and utilize the heating
Waste gas afterwards is heated to mouse tail, realizes whole mouse blood collection procedure integration.Meanwhile, using hot-air to mouse
Tail-heating, water stain-free residual, it is to avoid the generation of haemolysis.
As shown in fig. 7, in one embodiment, according to the mouse blood-taking device of various implementation methods of the invention, further
First support bar 56 and second support bar 20 including isolation material.It is spacing that mouse is fixed in one end of wherein first support bar 56
Under the stainless steel flat plate 41 of component, the other end is provided with sliding bar 14；One end of second support bar 20 is provided with sliding groove 15,
The other end is fixed under blood collection unit 26.First support bar 56 is with second support bar 20 is by respective sliding bar 14 and slides
Dynamic groove 15 is slidably connected, and the two is formed and is slidably connected lever knot of the place as fulcrum with first support bar 56 and second support bar 20
Structure.
In one embodiment, blood collection unit 26 further includes magnet ring 21 and round iron piece 22, wherein magnet ring 21
The cylindrical housing exterior of blood collection unit 26 is enclosed within, blood collection can be arranged on along cylinder blanket upper and lower translation, round iron piece 22
Within the cylinder blanket of device 26, it is fixed under centrifuge shield 23.Attract when magnet ring 21 is translated up round iron piece 22 also with
Run up, while driving the centrifuge tube 24 in the centrifuge shield 23 on round iron piece 22 to move upwards.
Mouse blood-taking device according to present embodiment, when stainless steel flat plate 41 is promoted obliquely, spoke shape blade type breaks
Cercoids 36 and blood collection unit 26 are outward-dipping, remove spoke shape blade type docking device 36, push up magnet ring 21 by centrifuge tube
Set 23 is lifted up, and to centrifuge tube 23 of the insertion containing anti-coagulants in centrifuge shield, unclamps magnet ring 21, and placement spoke shape blade type breaks
Cercoids 36, oblique drop-down stainless steel flat plate 41, spoke shape blade type docking device 36 and blood collection unit 26 slope inwardly, driven gear
33 are coincide with driving gear 37 again, and its circular knife hole site is indicated on the outer of spoke shape blade 30 according to the amplification of convex lens 28
Scale rotation regulation driven gear 33.So, can be in order to take out the blood of collection.
In this case, the two of contact-making switch 13 contacts can be separately fixed at first support bar 56 and second
The opposite side of strut 20, it is ensured that when carrying out mouse blood sampling, two contacts, contact-making switch conducting, operating circuit operation；Take out
During the blood of collection, stainless steel flat plate 41 is pushed up so that two contact separations, contact-making switch disconnection, operating circuit stopping fortune
OK.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention
Within god and principle, any modification, equivalent for being made etc. should be included within the scope of the present invention.
Claims (10)
1. a kind of mouse blood-taking device, it is characterised in that including mouse limit assembly, docking device, and blood collection unit, its
In,
Mouse limit assembly, the four limbs for fixing mouse；
Docking device, for cutting off Mouse Tail-tip；
Blood collection unit, for providing vacuum environment, and contains the blood of mouse docking surface of a wound drippage.
2. mouse blood-taking device as claimed in claim 1, it is characterised in that the mouse limit assembly includes flat board and setting
Position-limit foot tweezer on flat board, flat board is metal material, and the lower surface of position-limit foot tweezer includes magnet piece.
3. mouse blood-taking device as claimed in claim 2, it is characterised in that the position-limit foot tweezer includes pin tweezer fixing end and pin
The opposite side of tweezer mobile terminal, pin tweezer fixing end and pin tweezer mobile terminal indent each other, pin tweezer mobile terminal can be made with respect to pin tweezer fixing end
Translational motion.
4. mouse blood-taking device as claimed in claim 3, it is characterised in that further include control unit, control unit include according to
The secondary shifting component being fixedly connected, translation component, and limit assembly, wherein,
Translation component, and pin tweezer mobile terminal elastic connection, for driving pin tweezer mobile terminal to do translational motion；
Shifting component, for triggering the translational motion of translation component, and determines translation direction；
Limit assembly, the position for limiting translation component.
5. mouse blood-taking device as claimed in claim 4, it is characterised in that control unit includes push pedal, closed slide, and V
Type pitches component, closed slide and pin tweezer fixing end and pin tweezer mobile terminal be vertically set on pin tweezer mobile terminal and pin tweezer fixing end it
On, push pedal is fixedly connected with V-type fork component by cylinder-shaped link, and the two ends of cylinder-shaped link are overlapped on closed slide respectively
On, a fixed sliding block is distinguished in the position that the two ends of cylinder-shaped link contact with closed slide, in cylinder-shaped link
The spring fixed seat elastic connection that portion passes through the middle position on a compression spring and pin tweezer not moved end, V-type fork component
Including one is long and the other is short Liang Ge branches, branch long implements into tabular clamp, the pocket matches on clamp and magnet piece, short point
Branch implements into inverted frustro-pyramidal, and end is by extension spring and pin tweezer mobile terminal elastic connection.
6. mouse blood-taking device as claimed in claim 1, it is characterised in that the docking device includes axis, rotating part, and
Not cutting blade, rotating part can be around middle shaft rotation, and cutting blade is not relatively fixed with axis, between along being circular layout having size on rotating part
Every at least two through holes of arrangement, big external diameter of the through hole internal diameter more than mouse tail end, small through hole internal diameter is small
In the external diameter of mouse tail end, the blade of cutting blade is not tangent with through hole.
7. mouse blood-taking device as claimed in claim 6, it is characterised in that the docking device further includes card, it is fixed
In the bottom of axis, insertion blood collection unit inside is fixed.
8. mouse blood-taking device as claimed in claims 6 or 7, it is characterised in that rotating part is disc, including upper and lower folded
The spoke shape blade and tail point position-arresting disk put, at least two through holes of size interval arrangement are arranged on tail point position-arresting disk, spoke shape
It is provided with blade and is corresponded and insertion tool bore coaxial one by one, the inner edge of insertion tool bore with least two through hole
There is blade, external diameter of the internal diameter more than mouse tail end.
9. mouse blood-taking device as claimed in claim 8, it is characterised in that the rotating part further includes driven gear,
It is socketed on spoke shape blade, in this case, the mouse blood-taking device further includes the master being coupled with driven gear
Moving gear and operating circuit, operating circuit are used to drive driving gear to rotate.
10. mouse blood-taking device as claimed in claim 9, it is characterised in that further include negative pressure of vacuum component and heating
Component, heating component is arranged on the gas outlet of negative pressure of vacuum component, gas outlet just to docking device upper surface, negative pressure of vacuum component
Air inlet connects blood collection unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710040013.5A CN106725982B (en) | 2017-01-18 | 2017-01-18 | A kind of mouse blood-taking device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710040013.5A CN106725982B (en) | 2017-01-18 | 2017-01-18 | A kind of mouse blood-taking device |
Publications (2)
| Publication Number | Publication Date |
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| CN106725982A true CN106725982A (en) | 2017-05-31 |
| CN106725982B CN106725982B (en) | 2018-10-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710040013.5A Expired - Fee Related CN106725982B (en) | 2017-01-18 | 2017-01-18 | A kind of mouse blood-taking device |
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| CN107655717A (en) * | 2017-10-17 | 2018-02-02 | 深圳市第二人民医院 | A kind of rat broken end takes blood operating desk |
| CN110721004A (en) * | 2019-10-30 | 2020-01-24 | 漯河医学高等专科学校 | Quick positioning and tightening device for laboratory mouse |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN106725982B (en) | 2018-10-26 |
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