CN107988208A - For extracting the sampler and sampling method of sample from FFPE histotomies - Google Patents
For extracting the sampler and sampling method of sample from FFPE histotomies Download PDFInfo
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- CN107988208A CN107988208A CN201711437604.2A CN201711437604A CN107988208A CN 107988208 A CN107988208 A CN 107988208A CN 201711437604 A CN201711437604 A CN 201711437604A CN 107988208 A CN107988208 A CN 107988208A
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- 238000005070 sampling Methods 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000006835 compression Effects 0.000 claims abstract description 45
- 238000007906 compression Methods 0.000 claims abstract description 45
- 238000012360 testing method Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 13
- 241000973497 Siphonognathus argyrophanes Species 0.000 claims description 8
- 230000008878 coupling Effects 0.000 claims description 8
- 238000010168 coupling process Methods 0.000 claims description 8
- 238000005859 coupling reaction Methods 0.000 claims description 8
- 235000014676 Phragmites communis Nutrition 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 5
- 238000003780 insertion Methods 0.000 claims description 5
- 230000037431 insertion Effects 0.000 claims description 5
- 238000000576 coating method Methods 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 230000001737 promoting effect Effects 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 abstract description 18
- 108020004707 nucleic acids Proteins 0.000 abstract description 18
- 150000007523 nucleic acids Chemical class 0.000 abstract description 18
- 230000008901 benefit Effects 0.000 abstract description 7
- 238000000926 separation method Methods 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 description 8
- 238000001574 biopsy Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 244000089486 Phragmites australis subsp australis Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000004247 hand Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
This application provides a kind of sampler for being used to extract sample from FFPE histotomies, including outer tube, inner tube, piston, piston rod, pressure cap, motor, the first compression spring, sampling suction nozzle and conial tube；The sampler has the function of similar to the pipetting function of syringe operation principle and spin friction separation sample, extracting solution is extracted first with pipetting function to drop on dewaxing and dried histotomy, then sampled with rotating sampling suction nozzle spin friction on the histotomy being immersed in extracting solution, subsequently the mixed liquor for being mixed with tissue sample is transferred in sample collection test tube using pipetting function；So as to avoid the shortcomings that artificial FFPE histotomy method for extracting nucleic acid and its advantage is remained, more preferable work efficiency is obtained, working strength is reduced and has reached lower cost.Present invention also offers a kind of sampling method for being used to extract sample from FFPE histotomies.
Description
Technical field
The present invention relates to technical field of molecular biology, is used to extract sample from FFPE histotomies more particularly, to one kind
The sampler and sampling method of product.
Background technology
In medical domain, medical genetics, personalized medicine and gene target are treated to become in modern medicine and are hopeful
New breakthrough point.For this reason, it may be necessary to which target tissue will be come from, (needs for being determined and being marked by professional in FFPE histotomies will be into one
Walk detection specific region) nucleic acid (DNA and RNA) separate, for the base in clinical medicine or bioscience research
Because of analysis.FFPE histotomies herein mean that formalin fixes paraffin-embedded tissue section.
Generally accepted, the nucleic acid extracted from flesh tissue is more suitable for genetic analysis in terms of Nucleic acid quality；However, use
The major limitation of flesh tissue is time and availability, particularly collects visceral organ tissue from patient.Therefore, in most feelings
It is available under condition to organize based on the histotomy handled through FFPE to be obtained in aspiration biopsy or operation.FFPE tissues have
The advantages of storage decades can still extract its nucleic acid (DNA and RNA) and can be used for genetic test at room temperature.Existing many reports
Road confirms the availability for the satisfaction that DNA and RNA is extracted from FFPE tissues；The nucleic acid of extraction is usually used in PCR, quantitative PCR, list
Nucleotide polymorphisms (SNP) analysis, SANGER sequencings and gene sequencing of new generation.In a foreseeable future, FFPE tissues are still
The first choice of tissue sample is preserved in clinical practice.
The extraction of nucleic acid in FFPE tissues is a multi-step process relative to more difficult with flesh tissue；Including
The extracting of the dewaxing of FFPE tissues, dry, scraping and nucleic acid, each step are all important for the quality of nucleic acid and yield extracted
's.The nucleic acid extraction kit supply of the FFPE tissues of existing many business currently on the market, but carried for being fixed on pathology
The less tissue of target tissue and area on slide, (its size may diminish to several mm for particularly tiny aspiration biopsy2), but
There is no efficient method and apparatus.The nucleic acid extraction of artificial FFPE histotomies will have multiple artificial steps：Very important person first
Work dewaxing is multiple, to be overturn in the tank that dewaxes, and is spontaneously dried in atmosphere after taking out histotomy, adds on FFPE histotomies
Upper nucleic acid extraction solution, scrapes tissue using aseptic operation blade and is made a collection of specimens by pipette.This artificial method is mesh
Most conventional methods in preceding biomedical laboratory.It is apparent that these manual steps expend the time, manual intensity is big
(the frequent movement of finger and wrist), the difficult meeting bigger particularly when the sample for having medium or big quantity needs timely processing.
In addition, when being placed on multiple histotomies in the tank that dewaxes, there are the risk of tissue cross contamination.Laser differential is from skill
Art is another method for being used to separate FFPE tissues on glass slide, laser differential from the advantages of be can be accurately separated it is micro-
Small tissue, but compared with conventional manual method, application cost is too high, and also it is more time-consuming, each section will be 20 minutes or so,
This obviously cannot meet the needs of a large amount of sample treatments of reality.Commercially available " automatic FFPE organizes nucleic acid in addition with several
Extraction system ", however, the separation of FFPE tissue samples is not a part for automation, will cut 5-10 from paraffin embedded tissues
Piece tissue, tissue mass is big, is not suitable for the FFPE tissues of aspiration biopsy, and cannot be optionally sequestered on tissue sections
Target tissue area.Therefore it is still most common skill in biomedical laboratory that above-mentioned artificial FFPE, which organizes method for extracting nucleic acid,
Art.
Therefore, the shortcomings that how avoiding artificial FFPE histotomy method for extracting nucleic acid and retain its advantage, obtain
More preferable work efficiency, reduces working strength and reaches the technology that lower cost is current those skilled in the art's urgent need to resolve
Problem.
The content of the invention
The object of the present invention is to provide a kind of sampler for being used to extract sample from FFPE histotomies, sampling dress
Put for FFPE histotomies by shifting extracting solution on the histotomy that obtains after dewaxing treatment and from dewaxing treatment
Isolated tissue sample on the histotomy obtained afterwards, the shortcomings that avoiding artificial FFPE histotomy method for extracting nucleic acid, is simultaneously
And retain its advantage, more preferable work efficiency is obtained, working strength is reduced and reaches lower cost.Another of the present invention
Purpose is to provide a kind of sampling method for being used to extract sample from FFPE histotomies.
To solve above-mentioned technical problem, technical solution provided by the invention is：
It is a kind of be used for from FFPE histotomies extract sample sampler, including outer tube, inner tube, piston, piston rod,
Pressure cap, motor, the first compression spring, sampling suction nozzle and the conial tube for installing the sampling suction nozzle；
Said inner tube is sheathed in the outer tube, and the bottom tube mouth of the outer tube is blocked by the first capping, and the conial tube is worn
First capping enters the outer tube and is fixed on by bearing in first capping thoroughly, and the bottom tube of said inner tube
The push pipe mouth of mouth and the conial tube is tightly connected；
The pressure cap is arranged in the upper orifice of the outer tube, and the pressure cap and the outer tube by boss with it is recessed
The mutual cooperation of groove causes the pressure cap to be only capable of up and down reciprocatingly sliding but can not rotating along the outer tube；
The motor is arranged in the pressure cap, and the inner wall of the shell of the motor and the pressure cap, which is fixed, to be connected
Connect, the output shaft of the motor and the upper end of the piston rod are sequentially connected by shaft coupling, the power cord of the motor
The inner penetrate the pressure cap and the electronic mechatronics；
The piston is arranged in inner tube and in sealing contact with the inner surface of said inner tube, and the piston is arranged at the work
On the lower end of stopper rod；
The push pipe mouth of said inner tube is blocked by the second capping, and the push pipe mouth of said inner tube is fixed with the described second capping to be connected
Connect, the piston rod is penetrated in the second capping insertion said inner tube, and second capping passes through boss with the piston rod
Mutual cooperation with groove enables the piston rod up and down reciprocatingly to be slided along the described second capping and drives second capping
And inner tube rotation；
It is arranged with the part being exposed at outside said inner tube of the piston rod for promoting the pressure cap to recover to first
Begin the first upper compression spring, and the upper end of first compression spring props up the shaft coupling, and the lower end of first compression spring props up described
Second capping；
The sampling suction nozzle is removably sheathed in the cone of the conial tube, and the lower end periphery of the sampling suction nozzle
It is provided with to face for the frictional force increased between the sampling suction nozzle and histotomy and the damage reduced to histotomy
The coating of wound.
Preferably, boss is provided with the outer wall of the pressure cap, set on the inner surface of the outer tube it is fluted, it is described
So that the pressure cap is only capable of up and down reciprocatingly sliding along the outer tube in the groove that boss on pressure cap is embedded on the outer tube
But it can not rotate.
Preferably, boss, the circumferential hole face of the described second through hole covered are provided with the outer surface of the piston rod
It is upper to set fluted, the piston rod is enabled in the groove in the boss of piston rod insertion second capping along described
Second capping is up and down reciprocatingly slided and drives the second capping rotation.
Preferably, the unloading assembly for sampling suction nozzle to be unloaded from the conial tube is further included；
The unloading assembly includes hand compression bar, the lantern ring, the second compression spring and support tube；
The support tube is fixed on the outer surface of the outer tube, the push pipe mouth and bottom tube mouth of the support tube respectively by
3rd capping is blocked with the 4th capping；
The hand compression bar sequentially passes through the 3rd capping, the support tube and the 4th capping from top to bottom；
The hand compression bar is provided with crowded reed on the part in the support tube, and second compression spring sleeve is arranged on institute
State on hand compression bar, the upper end of second compression spring props up the crowded reed, and the lower end of second compression spring props up the 4th envelope
Lid；
The lantern ring is arranged on the bottom of the hand compression bar, and the lantern ring is sheathed on the conial tube, and described
The internal diameter of the lantern ring is less than the outside diameter of the upper end of the sampling suction nozzle.
Preferably, the lower face of the sampling suction nozzle is the plane vertical with the longitudinal center line of the sampling suction nozzle.
Preferably, set in the outer peripheral surface of the pressure cap described for the amount of moving down of the pressure cap to be converted to
The liquid measure graduation mark of the liquid absorption amount of sampler.
A kind of sampling method for being used to extract sample from FFPE histotomies, using described in any one in above-mentioned
Sampler, and comprise the following steps：
1) pressure cap in the sampler is pressed, is then immersed the lower end of the sampling suction nozzle in extracting solution, so
After unclamp the pressure cap with by extracting solution suck said inner tube in, then by it is described sampling suction nozzle lower end taken out from extracting solution
Go out, then press the pressure cap again and the extracting solution that is stored in said inner tube of suction before is dropped in into dewaxing and dried
On histotomy, liquid to be extracted unclamps the pressure cap after emptying；
2) it is powered and operates the motor, the motor finally drives the sampling suction nozzle to rotate by being sequentially connected,
With rotating sampling suction nozzle spin friction on the histotomy being immersed in extracting solution, the tissue sample that spin friction separates
Product, which are mixed into extracting solution, obtains mixed liquor；
3) pressure cap is pressed, is then immersed the lower end of the sampling suction nozzle in the mixed liquor that step 2) obtains, so
After unclamp the pressure cap, then by it is described sampling suction nozzle lower end extracted out from mixed liquor, then press the pressing again
The mixed liquor that suction is stored in said inner tube before is discharged into sample collection test tube by cap, after liquid to be mixed empties described in release
Pressure cap.
This application provides it is a kind of be used for from FFPE histotomies extract sample sampler, including outer tube, inner tube,
Piston, piston rod, pressure cap, motor, the first compression spring, sampling suction nozzle and conial tube；The sampler, which has, is similar to note
The pipetting function of emitter operation principle and spin friction separation sample function, are extracted first with the pipetting function of the sampler
Extracting solution is dropped on dewaxing and dried histotomy, then the tissue with rotating sampling suction nozzle in extracting solution is immersed in
Spin friction samples in section, and the mixed liquor for being mixed with tissue sample subsequently is transferred to sample using the pipetting function of sampler
Product are collected in test tube；The step of the application is by integrating all manually nucleic acid extractions of FFPE histotomies in addition to dewaxing,
The shortcomings that avoiding artificial FFPE histotomy method for extracting nucleic acid and its advantage is remained, obtain preferably work effect
Rate, reduces working strength and has reached lower cost, and the sampler is simple in structure, easy to use, quickly, cost
It is low.Present invention also offers a kind of sampling method for being used to extract sample from FFPE histotomies.
Brief description of the drawings
Fig. 1 is a kind of sampler for being used to extract tissue sample from histotomy that the embodiment of the present invention provides
Structure diagram (does not cause ambiguity, shaft coupling, piston rod, hand compression bar and the non-section of the lantern ring) in Fig. 1 in order to see that figure is convenient；
Fig. 2 is the amplification plan view of the second capping in Fig. 1.
In figure：1 outer tube, 101 grooves, 2 inner tubes, 3 pistons, 4 piston rods, 401 boss, 5 pressure caps, 6 motor, 7 first
Compression spring, 8 sampling suction nozzles, 9 conial tubes, 10 hand compression bars, 11 lantern rings, 12 second compression springs, 13 support tubes, 14 crowded reeds, 15 shaft couplings,
16 first cappings, 17 second cappings, 18 bearings, 19 coatings.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, the technical solution in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
The part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill people
Member's all other embodiments obtained on the premise of creative work is not made, belong to the scope of protection of the invention.
In the description of the present invention, it is to be understood that term " " center ", " axial direction ", " radial direction ", " longitudinal direction ", " transverse direction ",
" length ", " width ", " on ", " under ", "front", "rear", "left", "right", " top ", " bottom ", " interior ", " outer ", " clockwise ", " inverse
The orientation or position relationship of the instructions such as hour hands " are based on orientation shown in the drawings or position relationship, are for only for ease of description originally
Invent and simplify description, rather than the device or element of instruction or hint meaning there must be specific orientation, with specific side
Position construction and operation, therefore be not considered as limiting the invention.
With reference to Fig. 1, Fig. 1 is that a kind of of the embodiment of the present invention offer is used to extract taking for tissue sample from histotomy
The structure diagram of sampling device；Fig. 2 is the amplification plan view of the second capping in Fig. 1.
This application provides a kind of sampler for being used to extract sample from FFPE histotomies, including outer tube 1, inner tube
2nd, piston 3, piston rod 4, pressure cap 5, motor 6, the first compression spring 7, sampling suction nozzle 8 and for install it is described sampling suction nozzle 8
Conial tube 9；
Said inner tube 2 is sheathed in the outer tube 1, and the bottom tube mouth of the outer tube 1 is by 16 closure of the first capping, the taper
Pipe 9 penetrates first capping 16 and enters the inside of outer tube 1 and be fixed on by bearing 18 in first capping 16, and institute
The push pipe mouth of the bottom tube mouth and the conial tube 9 of stating inner tube 2 is tightly connected；
The pressure cap 5 is arranged in the upper orifice of the outer tube 1, and the pressure cap 5 passes through boss with the outer tube 1
Mutual cooperation with groove causes the pressure cap 5 to be only capable of up and down reciprocatingly sliding but can not rotating along the outer tube 1；
The motor 6 is arranged in the pressure cap 5, and the shell of the motor 6 and the inner wall of the pressure cap 5 are consolidated
Fixed connection, the output shaft of the motor 6 and the upper end of the piston rod 4 are sequentially connected by shaft coupling 15, the motor 6
The inner of power cord penetrate the pressure cap 5 and be electrically connected with the motor 6；
The piston 3 is arranged in inner tube 2 and in sealing contact with the inner surface of said inner tube 2, and the piston 3 is arranged at institute
On the lower end for stating piston rod 4；
The push pipe mouth of said inner tube 2 is solid by 17 closure of the second capping, the push pipe mouth of said inner tube 2 and the described second capping 17
Fixed connection, the piston rod 4 are penetrated in the 17 insertion said inner tube 2 of the second capping, second capping 17 and the piston
Bar 4 can up and down reciprocatingly be slided along the described second capping 17 by the mutual cooperation piston rod 4 of boss and groove and band
Dynamic second capping 17 and inner tube 2 rotate；
Be arranged with the part being exposed at outside said inner tube 2 of the piston rod 4 for promote the pressure cap 5 recover to
Initial the first upper compression spring 7, the upper end of first compression spring 7 prop up the shaft coupling 15, and the lower end of first compression spring 7 is supported
State the second capping 17 in residence；
The sampling suction nozzle 8 is removably sheathed in the cone of the conial tube 9, and the lower end of the sampling suction nozzle 8
It is provided with outer circumferential surface for increasing the frictional force between the sampling suction nozzle 8 and histotomy and reducing to histotomy
Damage coating 19.
In one embodiment of the application, boss, the interior table of the outer tube 1 are provided with the outer wall of the pressure cap 5
Set fluted 101 on face, the boss on the pressure cap 5 is embedded in the groove 101 on the outer tube 1 so that the pressure cap
5 are only capable of up and down reciprocatingly sliding but can not rotating along the outer tube 1.
In one embodiment of the application, boss 401, second envelope are provided with the outer surface of the piston rod 4
Set fluted in the circumferential hole face of through hole on lid 17, the boss 401 of the piston rod 4 is embedded in second capping 17
Enable the piston rod 4 up and down reciprocatingly to be slided along the described second capping 17 in groove and drive 17 rotation of the second capping.
In one embodiment of the application, the unloading for sampling suction nozzle 8 to be unloaded from the conial tube 9 is further included
Component；
The unloading assembly includes hand compression bar 10, the lantern ring 11, the second compression spring 12 and support tube 13；
The support tube 13 is fixed on the outer surface of the outer tube 1, the push pipe mouth and bottom tube mouth of the support tube 13
Blocked respectively by the 3rd capping and the 4th capping；
The hand compression bar 10 sequentially passes through the 3rd capping from top to bottom, the support tube 13 and the 4th covers；
The hand compression bar 10 is provided with crowded reed 14, second compression spring 12 on the part in the support tube 13
It is sheathed on the hand compression bar 10, the upper end of second compression spring 12 props up the crowded reed 14, under second compression spring 12
End props up the 4th capping；
The lantern ring 11 is arranged on the bottom of the hand compression bar 10, and the lantern ring 11 is sheathed on the conial tube 9,
And the internal diameter of the lantern ring 11 is less than the outside diameter of the upper end of the sampling suction nozzle 8.
In one embodiment of the application, it is described sampling suction nozzle 8 lower face be with it is described sampling suction nozzle 8 axial direction in
The vertical plane of heart line.
Set and be used for the pressure cap 5 in one embodiment of the application, in the outer peripheral surface of the pressure cap 5
The amount of moving down be converted to the sampler liquid absorption amount liquid measure graduation mark.
Present invention also provides a kind of sampling method for being used to extract sample from FFPE histotomies, in above-mentioned
Sampler described in any one, and comprise the following steps：
1) pressure cap 5 in the sampler is pressed, then immerses the lower end of the sampling suction nozzle 8 in extracting solution,
Then the pressure cap 5 is unclamped so that extracting solution is sucked in said inner tube 2, then by the lower end of the sampling suction nozzle 8 from extraction
Extracted out in liquid, then press again the pressure cap 5 by the extracting solution that is stored in said inner tube 2 of suction before drop in dewaxing and
On dried histotomy, liquid to be extracted unclamps the pressure cap 5 after emptying；
2) it is powered and operates the motor 6, the motor 6 finally drives the sampling suction nozzle 8 to revolve by being sequentially connected
Turn, with the rotating sampling spin friction on the histotomy being immersed in extracting solution of suction nozzle 8, the group that spin friction separates
Tissue samples, which are mixed into extracting solution, obtains mixed liquor；
3) pressure cap 5 is pressed, then immerses the lower end of the sampling suction nozzle 8 in the mixed liquor that step 2) obtains,
Then the pressure cap 5 is unclamped, is then extracted the lower end of the sampling suction nozzle 8 from mixed liquor out, then again described in pressing
The mixed liquor that suction is stored in said inner tube 2 before is discharged into sample collection test tube by pressure cap 5, pine after liquid to be mixed empties
Open the pressure cap 5.
The method and apparatus of not detailed description are the prior art in the application, are repeated no more.
The explanation of above example is only intended to help to understand method and its core concept of the invention.It should be pointed out that pair
For those skilled in the art, without departing from the principle of the present invention, the present invention can also be carried out
Some improvement and modification, these are improved and modification is also fallen into the protection domain of the claims in the present invention.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or use the present invention.
A variety of modifications for these embodiments are it will be apparent that defined herein one for those skilled in the art
As principle can realize in other embodiments without departing from the spirit or scope of the present invention.Therefore, the present invention will
It will not be intended to be limited to the embodiments shown herein, and be to fit to consistent with the principles and novel features disclosed herein
Widest range.
Claims (7)
1. a kind of sampler for being used to extract sample from FFPE histotomies, it is characterised in that including outer tube, inner tube, work
Plug, piston rod, pressure cap, motor, the first compression spring, sampling suction nozzle and the conial tube for installing the sampling suction nozzle；
Said inner tube is sheathed in the outer tube, and the bottom tube mouth of the outer tube is blocked by the first capping, and the conial tube penetrates institute
State the first capping into the outer tube and by bearing be fixed on it is described first capping on, and the bottom tube mouth of said inner tube with
The push pipe mouth of the conial tube is tightly connected；
The pressure cap is arranged in the upper orifice of the outer tube, and the pressure cap and the outer tube pass through boss and groove
Cooperate so that the pressure cap is only capable of up and down reciprocatingly sliding but can not rotating along the outer tube；
The motor is arranged in the pressure cap, and the shell of the motor is fixedly connected with the inner wall of the pressure cap,
The output shaft of the motor and the upper end of the piston rod are sequentially connected by shaft coupling, the power cord of the motor it is interior
End penetrates the pressure cap and the electronic mechatronics；
The piston is arranged in inner tube and in sealing contact with the inner surface of said inner tube, and the piston is arranged at the piston rod
Lower end on；
The push pipe mouth of said inner tube is blocked by the second capping, and the push pipe mouth of said inner tube is fixedly connected with the described second capping, institute
State piston rod to penetrate in the second capping insertion said inner tube, second capping passes through boss and groove with the piston rod
Mutual cooperation enable the piston rod along described second capping up and down reciprocatingly slide and drive it is described second capping and inner tube
Rotation；
It is arranged with the part being exposed at outside said inner tube of the piston rod for promoting the pressure cap to recover to initial
First compression spring of position, the upper end of first compression spring prop up the shaft coupling, and the lower end of first compression spring props up described second
Capping；
The sampling suction nozzle is removably sheathed in the cone of the conial tube, and the lower end periphery of the sampling suction nozzle is to face
On be provided with for increasing the frictional force between the sampling suction nozzle and histotomy and reducing damage to histotomy
Coating.
2. sampler according to claim 1, it is characterised in that boss, institute are provided with the outer wall of the pressure cap
State set on the inner surface of outer tube it is fluted, so that described press in the groove that boss on the pressure cap is embedded on the outer tube
Pressure cap is only capable of up and down reciprocatingly sliding but can not rotating along the outer tube.
3. sampler according to claim 1, it is characterised in that boss is provided with the outer surface of the piston rod,
Set in the circumferential hole face of through hole in second capping it is fluted, in the boss of piston rod insertion second capping
Groove in enable the piston rod along described second capping up and down reciprocatingly slide and drive it is described second capping rotate.
4. sampler according to claim 1, it is characterised in that further include for suction nozzle will to be sampled from the conial tube
On the unloading assembly that unloads；
The unloading assembly includes hand compression bar, the lantern ring, the second compression spring and support tube；
The support tube is fixed on the outer surface of the outer tube, and the push pipe mouth and bottom tube mouth of the support tube are respectively by the 3rd
Capping is blocked with the 4th capping；
The hand compression bar sequentially passes through the 3rd capping, the support tube and the 4th capping from top to bottom；
The hand compression bar is provided with crowded reed on the part in the support tube, and second compression spring sleeve is arranged on the hand
On compression bar, the upper end of second compression spring props up the crowded reed, and the lower end of second compression spring props up the 4th capping；
The lantern ring is arranged on the bottom of the hand compression bar, and the lantern ring is sheathed on the conial tube, and the lantern ring
Internal diameter be less than it is described sampling suction nozzle upper end outside diameter.
5. sampler according to claim 1, it is characterised in that the lower face of the sampling suction nozzle is and the sampling
The vertical plane of the longitudinal center line of suction nozzle.
6. sampler according to claim 1, it is characterised in that set and be used in the outer peripheral surface of the pressure cap
The amount of moving down of the pressure cap is converted to the liquid measure graduation mark of the liquid absorption amount of the sampler.
A kind of 7. sampling method for being used to extract sample from FFPE histotomies, it is characterised in that usage right requirement 1~6
In any one described in sampler, and comprise the following steps：
1) pressure cap in the sampler is pressed, is then immersed the lower end of the sampling suction nozzle in extracting solution, Ran Housong
The pressure cap is opened so that extracting solution is sucked in said inner tube, then extracts the lower end of the sampling suction nozzle from extracting solution out,
Then the pressure cap is pressed again, and the extracting solution that suction is stored in said inner tube before is dropped in into dewaxing and dried group
Knit in section, liquid to be extracted unclamps the pressure cap after emptying；
2) it is powered and operates the motor, the motor finally drives the sampling suction nozzle to rotate by being sequentially connected, with rotation
The sampling suction nozzle spin friction on the histotomy being immersed in extracting solution turned, the tissue sample that spin friction separates mix
Enter and mixed liquor is obtained in extracting solution；
3) pressure cap is pressed, is then immersed the lower end of the sampling suction nozzle in the mixed liquor that step 2) obtains, Ran Housong
The pressure cap is opened, then extracts the lower end of the sampling suction nozzle from mixed liquor out, then pressing the pressure cap again will
The mixed liquor that suction is stored in said inner tube before is discharged into sample collection test tube, and liquid to be mixed unclamps the pressing after emptying
Cap.
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| CN201711437604.2A CN107988208A (en) | 2017-12-26 | 2017-12-26 | For extracting the sampler and sampling method of sample from FFPE histotomies |
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| CN201711437604.2A Pending CN107988208A (en) | 2017-12-26 | 2017-12-26 | For extracting the sampler and sampling method of sample from FFPE histotomies |
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| CN110646233A (en) * | 2019-11-22 | 2020-01-03 | 广州赛太特生物医学科技有限公司 | Novel cell diagnosis is with quantitative sample device |
| CN110686922A (en) * | 2019-10-10 | 2020-01-14 | 北京大学第一医院 | Split type low-temperature tissue divider |
| CN113146702A (en) * | 2021-04-26 | 2021-07-23 | 朱忠俊 | Biological slicing device for columnar object |
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| CN110646233A (en) * | 2019-11-22 | 2020-01-03 | 广州赛太特生物医学科技有限公司 | Novel cell diagnosis is with quantitative sample device |
| CN113146702A (en) * | 2021-04-26 | 2021-07-23 | 朱忠俊 | Biological slicing device for columnar object |
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Application publication date: 20180504 |