CN106720944A

CN106720944A - A kind of livestock and poultry microbial forage additive

Info

Publication number: CN106720944A
Application number: CN201611170781.4A
Authority: CN
Inventors: 刘先华
Original assignee: Chongqing One Point Nine Package Enterprise Management Consulting Co Ltd
Current assignee: Chongqing One Point Nine Package Enterprise Management Consulting Co Ltd
Priority date: 2016-12-16
Filing date: 2016-12-16
Publication date: 2017-05-31

Abstract

A kind of livestock and poultry microbial forage additive, it is characterised in that：Probiotics is prepared based on lactobacillus acidophilus, its component is by weight calculating, containing lactobacillus acidophilus 45~60%, German-style lactobacillus 0.5%~25%, bacillus subtilis 12~15%, calcium superphosphate 15~18%, photosynthetic bacteria 18~20%, Bacillus acidi lactici 2~5%, summation is 100%.

Description

A kind of livestock and poultry microbial forage additive

Technical field

The present invention relates to a kind of feed probiotics, belong to field of fodder, specifically a kind of livestock and poultry Tiny ecosystem Feed addictive.

Background technology

Probiotics is a kind of novel fodder additive for growing up in recent years, because it has nontoxic, harmless, nothing Residual, it is pollution-free the advantages of, while have prevent and cure diseases, improve efficiency of feed utilization, improve the effect such as breeding performonce fo animals, and The shortcomings of overcoming flora imbalance produced by antibiotic, suprainfection and drug resistance, is widely used to herding, aquaculture In industry.Feeding micro-ecological preparation is, using known beneficial microbe, to be made through the special process such as culture, fermentation, dry, processing Containing viable bacteria and for the biological agent or active bacteria formulation of animal feeding.Other titles for example microorganism fodder additive, probiotic, Probiotics, prebiotics, Synbiotics etc. belong to feeding micro-ecological preparation category.

Early in 1981, professor Wei Xi just pointed out " epoch after antibiotic will be probiotics epoch ".Micro- life State composite bacteria preparation is from the point of view of foreign countries' exploitation and using effect, and composite bacteria preparation is more preferable compared with the action effect of single bacterial strain.It is multiple Closing bacteria preparation typically all has synergy, therefore shows from single bacterial strain probiotics to compound micro-ecological preparation development Trend；Stability of its production bacterial strain is always a great problem, in putting into feed instead of viable bacteria form in hypopus form Feeding animals are to be worth the problem inquired into.Technique for gene engineering is recycled, makes the probiotics of dormant state special in animal body Positioning puts activation, to have played expected effect.Have not yet to see many bacterium solid state fermentation brewex's grains production feeding micro-ecological preparations Relevant report.

The content of the invention

The present invention is intended to provide a kind of livestock and poultry microbial forage additive, rises for viable bacteria in existing probiotics Single and not strong to the adaptability of the animal alimentary canal environment shortcoming of effect, there is provided it is feeding micro- that a kind of multi-cultur es are used cooperatively Ecological agent, said preparation has very strong adaptability to animal alimentary canal environment, is conducive to maintaining micro- life of environment in animal intestinal tract State is balanced and can be greatly enhanced animal immunizing power.

The technical scheme is that, a kind of livestock and poultry plant microecology formulation products, it is characterised in that：With acidophilus breast bar Probiotics is prepared based on bacterium, its component contains lactobacillus acidophilus 45~60%, German-style lactobacillus by weight calculating 0.5%~25%, bacillus subtilis 12~15%, calcium superphosphate 15~18%, photosynthetic bacteria 18~20%, Bacillus acidi lactici 2 ~5%, summation is 100%, is operated in accordance with the following steps：

Step one, lactobacillus acidophilus, German-style lactobacillus, bacillus subtilis, photosynthetic bacteria, the expansion training of Bacillus acidi lactici Support, seed selection lactobacillus acidophilus, German-style lactobacillus, bacillus subtilis, photosynthetic bacteria, the good strain of Bacillus acidi lactici are placed in culture medium Cultivated in 30 to 40 degrees Celsius of temperature 20 to 28 hours and suspension is obtained；Described culture medium includes 8 to 12 parts of peptone, 5 to 7 parts of yeast extract, 10 to 14 parts of beef extract, 14 to 18 parts of glucose, 4 to 6 parts of sodium acetate, 2 to 2.5 parts of ammonium citrate, 0.04 to 0.06 part of 0.55 to 0.6 part of 0.8 to 1.2 part of Tween80, MgSO47H2O, MnSO44H2O, K2HPO41.8 To 2.2 parts, 16 to 18 parts of agar powder, 900 to 1000 parts of water, the culture rate of vaccination of described bacterium is 8% to 12%；

Step 2, the selection of the wall material of state preparation, after Amplification Culture terminates, suspension are filtered, and after filtration outstanding The beta-schardinger dextrin of the 10% to 15% of bacteria suspension total amount, 12% to 18% shitosan, 3% to 5% after addition is accounted for and filtered in liquid WPC, 8% to 10% porous-starch is simultaneously well mixed；Or addition account for filtering after lactobacillus acidophilus suspension it is total The shitosan of the 12% to 20% of amount, 5% to 8% WPC, 6% to 10% carbohydrate simultaneously mixes equal It is even, that is, obtain adding the lactobacillus acidophilus suspension after embedding medium；

Step 3, by add embedding medium after lactobacillus acidophilus suspension be dried after obtain lactobacillus acidophilus Tiny ecosystem Finished dosage form.

Livestock and poultry microbial forage additive is 1: 80~100, the culture medium for being used with the mixed proportion of normal diet PH acid-base values initial value be 5.5, drying means in described step three is low temperature spray drying method, after addition embedding medium Lactobacillus acidophilus suspension be put into sprayer unit, the inlet temperature of device is 85 to 95 degrees Celsius, and outlet temperature is 55 to 65 Degree Celsius, and the average time that the lactobacillus acidophilus suspension added after embedding medium receives to spray in sprayer unit is 3 to 6 seconds.

Drying means in described step three is freeze-drying, and the lactobacillus acidophilus suspension added after embedding medium is put In subzero less than 40 degrees Celsius of low temperature refrigerator pre-freeze 3 to 7 hours, the freezing that vacuum is 15 to 25 handkerchiefs is then placed on again Freeze-drying 30 to 40 hours in drying device.

A kind of livestock and poultry microbial forage additive of the present invention, its advantage is：Improve the ring in poultry house Border, improves livestock and poultry and exempts from service power, effectively prevents the generation of various diseases, improves pregnancy rate, farrowing rate, survival rate；Using the present invention The poultry that additive for microbe feedstuff technology is fed, can make beneficial active microorganism form benign in the enteron aisle of animal Microbiologic population, so as to suppress the growth of harmful levels of pathogens, has obvious conditioning functions to animal gastrointestinal tract, improves exempting from for animal Epidemic disease ability, so as to reach prevention disease；Improve food conversion ratio, reduce feedstuff-meat ratio, feedstuff-egg ratio, reduces cost.

Specific embodiment

Embodiment 1

A kind of livestock and poultry microbial forage additive, it is characterised in that：Probiotics is prepared based on lactobacillus acidophilus, Its component contains lactobacillus acidophilus 45~60%, German-style lactobacillus 0.5%~25%, bacillus subtilis by weight calculating 12~15%, calcium superphosphate 15~18%, photosynthetic bacteria 18~20%, Bacillus acidi lactici 2~5%, summation is 100%, according to such as Lower step is operated：

Claims

1. a kind of livestock and poultry microbial forage additive, it is characterised in that：Probiotics is prepared based on lactobacillus acidophilus, its Component contains lactobacillus acidophilus 45~60%, German-style lactobacillus 0.5%~25%, bacillus subtilis 12 by weight calculating ~15%, calcium superphosphate 15~18%, photosynthetic bacteria 18~20%, Bacillus acidi lactici 2~5%, summation is 100%, according to as follows Step is operated：

Step one, lactobacillus acidophilus, German-style lactobacillus, bacillus subtilis, photosynthetic bacteria, the Amplification Culture of Bacillus acidi lactici, choosing Educate lactobacillus acidophilus, German-style lactobacillus, bacillus subtilis, photosynthetic bacteria, the good strain of Bacillus acidi lactici be placed in culture medium 30 to Cultivated in 40 degrees Celsius of temperature 20 to 28 hours and suspension is obtained；Described culture medium includes 8 to 12 parts of peptone, yeast 5 to 7 parts of extract, 10 to 14 parts of beef extract, 14 to 18 parts of glucose, 4 to 6 parts of sodium acetate, 2 to 2.5 parts of ammonium citrate, 0.04 to 0.06 part of 0.55 to 0.6 part of 0.8 to 1.2 part of Tween80, MgSO47H2O, MnSO44H2O, K2HPO 41.8 to 2.2 parts, 16 to 18 parts of agar powder, 900 to 1000 parts of water, the culture rate of vaccination of described bacterium is 8% to 12%；

Step 2, the selection of the wall material of state preparation, after Amplification Culture terminates, suspension are filtered, and in suspension after filtration Addition accounts for the beta-schardinger dextrin of 10% to 15% of bacteria suspension total amount after filtering, 12% to 18% shitosan, 3% to 5% breast Clear protein concentrate, 8% to 10% porous-starch is simultaneously well mixed；Or addition accounts for lactobacillus acidophilus suspension total amount after filtering 12% to 20% shitosan, 5% to 8% WPC, 6% to 10% carbohydrate is simultaneously well mixed, i.e., Obtain adding the lactobacillus acidophilus suspension after embedding medium；

Step 3, by add embedding medium after lactobacillus acidophilus suspension be dried after obtain lactobacillus acidophilus probiotics Finished product.

2. livestock and poultry microbial forage additive as claimed in claim 1, it is characterised in that：Poultry micro-ecological preparation with it is common The mixed proportion of feed is 1: 80~100, and the pH acid-base value initial values of the culture medium for being used are 5.5, in described step three Drying means be low temperature spray drying method, by add embedding medium after lactobacillus acidophilus suspension be put into sprayer unit, device Inlet temperature be 85 to 95 degrees Celsius, outlet temperature be 55 to 65 degrees Celsius, and addition embedding medium after lactobacillus acidophilus hang The average time that liquid receives spraying in sprayer unit is 3 to 6 seconds.

3. livestock and poultry microbial forage additive according to claim 1, it is characterised in that：It is dry in described step three Drying method is freeze-drying, and the lactobacillus acidophilus suspension added after embedding medium is placed in subzero less than 40 degrees Celsius of Low-temperature Ice Case pre-freeze 3 to 7 hours, freeze-drying 30 to 40 is small during the freeze drying plant that vacuum is 15 to 25 handkerchiefs is then placed on again When.