CN106719766A - A kind of method of efficient preventing and treating lily bulb penicilliosis - Google Patents
A kind of method of efficient preventing and treating lily bulb penicilliosis Download PDFInfo
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- CN106719766A CN106719766A CN201710030709.XA CN201710030709A CN106719766A CN 106719766 A CN106719766 A CN 106719766A CN 201710030709 A CN201710030709 A CN 201710030709A CN 106719766 A CN106719766 A CN 106719766A
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- lily
- bulb
- penicilliosis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a kind of method of efficient preventing and treating lily storage period bulb penicilliosis, based on composite reagent principle.This patent is by several single medicaments of preferred pair and its method for compounding, screening can efficiently prevent and treat the pharmaceutical agent combinations of the rot that lily ball storage period is caused by penicillium commune (Penicillium commune), this patent not only solves the single chemical control bulb mould germ of conventional long-term use, dosing is caused gradually to increase, the problem for developing immunity to drugs, solve the antimicrobial combination and bactericide usage amount for causing rot by penicillium commune simultaneously, to reducing the resistance to the action of a drug, reduces cost, hence it is evident that synergy has important practical value.
Description
Technical field
The present invention relates to effectively prevent and treat the lily bulb mould caused by penicillium commune Penicillium commune
Disease, screening prevents and treats one or more medicament of the disease.
Background technology
Lily is the general name of Liliaceae (Liliaceae) lilium (Lilium) plant, and property is sweet for its, be slightly cold, return lung, the heart,
Stomach, has functions that Yin nourishing and lung moistening, clearing away the heart fire and tranquillizing.In recent years, with the expansion of lily planting area, long-term continuous cropping in addition,
In lily storage and the season of growth, often have a large amount of bulbs by fungal infection, using general bactericidal agent (such as carbendazim) after, generally
Substantial amounts of Penicillium notatum can be produced, causes bulb to rot, it is right in the quality and its growth course of lily ball to have had a strong impact on
The resistance capacity of other pathogens, therefore for filtering out the bactericide of efficiently preventing and treating lily storage period bulb Penicillium notatum, reduce
Cost, improves bulb yield and quality is significant.
Wang Yan etc. (2011) carries out seed borne fungi to the lily bulb of the province of Gansu etc. 6, as a result shows, lily bulb carries true
Strain class is more and quantity is larger, wherein predominantly Penicillium notatum, sickle-like bacteria, bread mold etc..Penicilliosis is to cause lily storage period
Major Diseases (Zhu Mingde, 2004), can cause lily withered and rotten in Adult plant and storage period bulb.Zhu Haiyan (2012)
Propose that lily bulb penicilliosis pathogen is invaded by the wound on lily bulb, cause aggrieved bulb brown scab occur, it
After grow the mould layer of blue-green, gradually rot, and can mutually be infected between bulb.Zhang Yi (2010) points out to be planted in lily bulb
It is preceding with the desinsections such as 1 ‰ captans, Bravo, carbendazim, potassium permanganate (bacterium) agent aqueous solution soaking 30min, plantation can after drying
To prevent and treat bulb mould.Wang Caixia etc. (2006) proposes to be carried out to adopting rear lily with 50mg/L, 40 DEG C of kresoxim-methyl
The controllable lily storage period penicilliosis penicillium cyclopium of 1min immersion treatments.Penicillium notatum infects to mostly occur to be adopted in growth period or bulb
In receipts, packaging, transport, storage process.The kind ball surface infected by Penicillium has a mould layer of blue-green, partial decomposition, and rotten portion is
Brown, in irregular shape, depression, scab edge black is obvious with strong part circle, the upper raw mould layer of dark green, under bulb store quality
Drop, makes bulb lose value or makes plant strain growth slow (Zhang Yi, 2010), and single the long-term of medicament largely uses so that cause of disease
The adaptability that bacterium rapidly generates to medicament, preventive and therapeutic effect is substantially reduced, and not only increases cost, while also to ecological environment
And organism causes serious destruction and threat.This patent isolates pathogenic bacteria penicillium commune to storage tiger lily, and external
There are no to this kind of report of germ chemical control, therefore this patent is using the method for preferred list composite reagent, filter out wide spectrum,
Efficient bactericide compounded component, the rotten penicillium commune of lily ball basal disc is caused for preventing and treating, reduces the list of pathogen
Medicament tolerance and the resistance to the action of a drug have important practical value.
Leading reference：
1) Wang Yan, Tao of shutting out connects middle school, Chen Honggang, Jiang Haibo, Wang Huizhen, Zhang Yanhong, Zhu field field lily bulb band fungies
Detection [J] Gansu Chinese of Traditional Chinese Medicine journal, 2011,28 (2)：48-51.
2) the peaceful lilies storage disease of Zhu Mingde, Pan Qiyun, Deng Jianling, Tang Xiang and its research [J] the Shanghai of anti-corrosion measure
Agricultural science and technology, 2004, (2)
3) preventing and treating [J] the Qinghai science and technology of the common disease pest plague of rats of lilies of being lost, 2010, (4) are opened
4) Zhu Haiyan Lilium brownii var viridulums bulb-rot Pathogen identification and indoor Study on Fungicide Toxicity [D] Agriculture in Hunan are big
Learn：Agricultural University Of Hunan, 012.
5) Wang Caixia, the red kresoxim-methyls of Bi Yang, Ge Yong are learned control [J] the Gansu Agriculture Universities of lily bulb penicilliosis
Report, 2006, (5)
6) identification of Zhao Hong Pathogens Isolated from Rotted Gladiola Bulbs and its pathogenicity [J] Gansu Agriculture Universities journal, 1997, (2)
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of method of effective preventing and treating lily bulb penicilliosis.By fungi
Growth rate method carries out the screening of medicament, determines Prochloraz and its can effectively prevent lily from preserving green grass or young crops with Prochloraz compound drug
The mycelial growth of mildew pathogen, contains its spore dispersal, to preventing and treating the disease there is important practical to be worth and application life
Produce value.
To achieve these goals, the present invention uses following technical measures：
1) separated from the strong intersection of tiger lily scale disease using tissue isolation, purify pathogen, it is big that scale is cut into 5 × 5mm
It is small, through 75% ethanol postincubation 1min, aseptic water washing 5 times, each 30s.
2) basis of microscopic observation its form, and photomicrograph are placed in sterile needle picking hypha,hyphae.
3) pastille flat board is made：Each bactericide sets 5 concentration gradients respectively, be configured to 500,1000,2000,4000,
The pastille PDA plate of 8000 extension rates, is well mixed.1h or so is irradiated after sealing under uviol lamp, germ contamination is prevented.
4) after strains tested grows 5d in PDA culture medium, under aseptic condition, vigorous area's punching is grown from colony edge
Device (d=8mm) is beaten and takes bacteria cake of the same age, and connecing acicula with sterilization is put on above-mentioned pastille PDA plate well prepared in advance bacteria cake, seals
Membrana oralis is sealed.It is placed in 25 DEG C of incubators and cultivates, is inverted.After 5 days, colony diameter is measured with crossing method, calculate and suppress life
Rate long.
Brief description of the drawings
Fig. 1 pathogens growing state in PDA culture medium；
Pathogen conidium form feature under Fig. 2 microscopes；
Symptom after the inoculation of Fig. 3 Sorbonne scales；
(Fig. 4-1 is benzene bacterium to the effect that the mono- medicaments of Fig. 4 suppress to lily bulb storage period penicilliosis pathogen mycelial growth
Spirit, Fig. 4-2 is kresoxim-methyl, and Fig. 4-3 is Prochloraz, and Fig. 4-4 is iprodione)；
Control：CK；Treatment：Single medicament extension rate 500,1000,2000,4000,8000
Effect that Fig. 5 compound drugs suppress to mycelial growth (Fig. 5-1 is benzene bacterium Prochloraz, and Fig. 5-2 is the fresh kresoxim-methyl of miaow,
Fig. 5-3 is the fresh iprodione of miaow, and Fig. 5-4 is benzene bacterium iprodione, and Fig. 5-5 is ether bacterium benomyl, and Fig. 5-6 is different bacterium ether bacterium
Ester).
Control：CK；Treatment：Compound drug extension rate 250,500,1000,2000,4000,8000
Specific embodiment
The sick method of lily bulb penicillium communne is effectively prevented and treated, its step is：
The separation of 1 pathogen, purifying
The rotten strong intersection of bulb scale disease of tiger lily is separated using conventional organization partition method.Scale distilled water flushing
5min, 75% alcohol-pickled concussion 1min is placed on superclean bench by scale, and with aseptic water washing 5 times, each 30s cuts
Into 5 × 5mm blockages, it is placed in PDA culture medium, 25 DEG C, dark culturing.After 5 days, with transfer needle by fungus colony edge mycelia
Picking in PDA culture medium, 25 DEG C, dark culturing.Previous step is repeated, until growing single kind of form bacterium in PDA culture medium
Fall.
2 Pathogens are identified
(1) strains tested colony morphological observation
Each inoculation after isolating and purifying is cultivated on PDA plate under 25 DEG C of dark conditions, and each bacterial strain is observed after 5d
The proterties, Taking Pictures recording such as colonial morphology, color, mycelial growth situation.
(2) the microcosmic microscopic features observation of examination bacterial strain
Fungi carries out Liquid Culture, and basis of microscopic observation its form, and photomicrograph are placed in sterile needle picking.
3 Pathogenic Tests
Needed to carry out tieback to the disease fungus for obtaining according to Koch's Postulates, carry out Pathogenic Tests.Scale uses 75%
After alcohol surface sterilization, aseptic water washing 5 times, with sterilizing pipette tips in scale acanthopore, is inoculated with penicillium commune bacterium solution, soaks 20min,
Control is to be placed in 20min in sterilized water with the bulb of aseptic pipette tips acanthopore, and each treatment in triplicate, is placed in 25 DEG C, and dark is protected
Wet culture.Record incidence, takes pictures daily.The bulb of inoculation morbidity is separated and identified.
4 Chemicals
The fungistatic effect of reagent agent is determined using mycelial growth rate method.
Microbiocidal composition and producer's list
| Pesticide name | Manufacturer |
| 50% benomyl wettable powder | Jiangsu Lam Fung Biological Chemical Co., Ltd |
| 50% water dispersion granules agent of Kresoxim (emerald green shellfish) | German BASF |
| 50% prochloraz-manganese chloride complex wettable powder (makes hundred work() | Huifeng Agricultural Chemistry Co., Ltd., Jiangsu |
| 50% iprodione wettable powder (flutter greatly because) | Dongguan City RuiDeFeng Biology Science Co., Ltd |
The corresponding Developing restraint rate of bactericide difference extension rate
Claims (2)
1. the method for effectively preventing and treating lily bulb penicilliosis, it is characterised in that comprise the following steps：
(1) rules of organization are separated, purify pathogen
(2) pathogen microscopy
(3) Pathogenic Tests：Healthy lily cultivar ' Sorbonne ' scale with after 75% alcohol surface sterilization, aseptic water washing 5
Time, with sterilizing pipette tips acanthopore on scale, and 20min is inoculated with being dipped in bacterium solution, sets the blank (squama of sterilizing pipette tips acanthopore
Piece is dipped in 20min in sterilized water) control, moisturizing, each processes three repetitions, incidence is recorded daily, and take pictures.
(4) indoor Study on Fungicide Toxicity：Determine bactericide species, concentration, make pastille potato dextrose agar
(PDA) flat board, card punch is beaten and takes bacteria cake and be placed on pastille flat board, and control is placed in without in bactericide PDA culture medium, 25 DEG C, training
Support 5 days, crossing method measurement colony diameter calculates growth inhibition ratio.
2. it is according to claim 1 it is effective preventing and treating lily bulb penicilliosis method, it is characterised in that：Step (4) is described
Pastille flat board elder generation manufacturing process is that bactericide is diluted into 50 times of mother liquors, and 2mL, 1mL, 500 μ L, 250 μ are taken on superclean bench
L, 125 μ L mother liquors are added to 20mL and have sterilized in PDA culture medium (temperature control is at 60 DEG C -65 DEG C), be configured to 500,1000,
2000th, the pastille PDA plate of 4000,8000 extension rates, several medicaments are compounded two-by-two according to 1: 1 ratio, are made
50 times of mother liquors (every kind of medicament is configured to 100 times of liquid, is compounded in 1: 1 ratio, is regarded as entirety, is still 50 times of liquid),
After mixed medicine is uniform, at ultraviolet lower sterilized 1 hour after sealing.The strains tested of growth 5d is taken under aseptic condition, from colony edge
Grow vigorous area's card punch (d=8mm) and beat and take bacteria cake of the same age, pastille PDA well prepared in advance is put into connecing acicula and taking bacteria cake
On flat board, cultivate 5 days.Prochloraz and its Prochloraz compounded combination are filtered out, wherein Prochloraz is 50% wettable powder, dilute
It is 250 times to release multiple, and antibacterial growth rate is 95.12%, and the antibacterial growth rate of benomyl, kresoxim-methyl, iprodione is up to
95.12%th, 96.34%, 97.56%, the mycelial growth of lily bulb Penicillium notatum can be effectively prevented, while having broad-spectrum sterilization
Effect, compounded combination can substantially reduce medicament usage amount and cost, reduce the resistance to the action of a drug of pathogen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710030709.XA CN106719766A (en) | 2017-01-17 | 2017-01-17 | A kind of method of efficient preventing and treating lily bulb penicilliosis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710030709.XA CN106719766A (en) | 2017-01-17 | 2017-01-17 | A kind of method of efficient preventing and treating lily bulb penicilliosis |
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| CN201710030709.XA Pending CN106719766A (en) | 2017-01-17 | 2017-01-17 | A kind of method of efficient preventing and treating lily bulb penicilliosis |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101595889A (en) * | 2009-05-07 | 2009-12-09 | 深圳诺普信农化股份有限公司 | A kind of bactericidal composition that contains iprodione |
| CN102047898A (en) * | 2010-12-20 | 2011-05-11 | 陕西韦尔奇作物保护有限公司 | Bactericidal composition containing prochloraz and methoxy acrylic acid ester type compound |
-
2017
- 2017-01-17 CN CN201710030709.XA patent/CN106719766A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101595889A (en) * | 2009-05-07 | 2009-12-09 | 深圳诺普信农化股份有限公司 | A kind of bactericidal composition that contains iprodione |
| CN102047898A (en) * | 2010-12-20 | 2011-05-11 | 陕西韦尔奇作物保护有限公司 | Bactericidal composition containing prochloraz and methoxy acrylic acid ester type compound |
Non-Patent Citations (2)
| Title |
|---|
| 朱维军: "保鲜剂在瓜菜贮藏中的应用", 《中国瓜菜》 * |
| 杨迎东等: "百合鳞茎青霉病防治药剂筛选", 《北方园艺》 * |
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