CN106718718A - Method of the phosphorus efficiency rate using kind is screened from multiple barley varieties - Google Patents
Method of the phosphorus efficiency rate using kind is screened from multiple barley varieties Download PDFInfo
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- CN106718718A CN106718718A CN201611012032.9A CN201611012032A CN106718718A CN 106718718 A CN106718718 A CN 106718718A CN 201611012032 A CN201611012032 A CN 201611012032A CN 106718718 A CN106718718 A CN 106718718A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
A kind of that method of the phosphorus efficiency rate using kind is screened from multiple barley varieties, seed selection grain barley variety of the same size is put into culture dish, sprouts to the seedling for taking root；The consistent seedling of growth is selected from seedling, is divided into two groups, pour ripe to barley with different phosphorus concentration nutrient solutions；Determine and calculate relative plant height, relative grain number per spike and the relative mass of 1000 kernel of each kind；Comprehensive Correlation, selects three several barley varieties of index comprehensive highest for phosphorus efficiency rate utilizes kind；And sprout to the secondary screening seedling for taking root, the consistent secondary screening seedling of growing way is taken, it is divided into two groups, water planting is carried out with different phosphorus concentration nutrient solutions, determine and calculate the relative dry matter weight of each kind, relative phosphorus content, relative chlorophyll content；Comprehensive Correlation, select three index comprehensive highests, 1 barley variety for phosphorus use efficiency with respect to highest kind.The barley variety that this method is finally filtered out, is further to improve existing kind, studies its biological characteristics and Mechanism of Physiological and Biochemical provides material.
Description
Technical field
The invention belongs to barley cultivation and breeding technical field, it is related to one kind that phosphorus efficiency rate is screened from multiple barley varieties
Using the method for kind.
Background technology
Barley is one of crops most ancient in the world, away from the cultivation history of the present existing thousands of years, be cultivated area most
One of big cereal crops.Whole world barley sown area is in about 96,000,000 hectares of the 1970's Mos（246000000）, annual production is near
180000000 tonnes, its sown area and total output are only second to wheat, and paddy rice, corn occupies cereal crops the 4th, mainly raises
Material, grain, brewing industry and medical industry raw material and health food.Barley is also one of China essentialspecies plant, China barley
Be distributed in raise crop most extensively, but major production areas Relatively centralized is mainly distributed on the Yangtze river basin, the Huanghe valley and Qinghai-Tibet
Plateau.In recent years, with the fast development of China's brewing industry, the demand to malting barley is increasing.Because phosphorus is plant
The required nutrient of growth, is also the constituent of important compound in plant, and participate in plant in many ways
Various physiology and metabolic process, in order to ensure high crop yield and stable yields, just must largely apply phosphate fertilizer.But phosphate fertilizer be it is a kind of not
Renewable resource, improves the amount of application of phosphate fertilizer, not only waste of resource, again meeting in the production process of barley in order to improve yield
Bring many environmental problems.Therefore, it will be the reason for solving these problems to screen and cultivate Tolerant to low P, phosphorus efficiency rate New Barley Variety
Think approach.
The content of the invention
In order to solve above-mentioned problems of the prior art, screened from multiple barley varieties the invention provides one kind
Phosphorus efficiency rate, by the combination of time of infertility sand culture and seedling stage water planting, sets up reliable, the easy phosphorus of barley using the method for kind
The screening technique of utilization ratio kind high.
To achieve the above object, the technical solution adopted in the present invention is：
A kind of that method of the phosphorus efficiency rate using kind is screened from multiple barley varieties, the screening technique specifically enters according to the following steps
OK：
a）Select full, seed size it is consistent the need for the barley variety that is screened, the culture for being covered with filter paper is placed in respectively
In ware, sprouted in illumination box to the seedling for taking root；
b）Sand culture primary dcreening operation：From step a）Each barley variety seedling in pick out the 2n plants of consistent seedling of growth, and by each
The seedling of kind is divided into two groups, two groups of seedling is poured respectively with the nutrient solution of two kinds of different phosphorus concentrations, until barley is ripe；
c）Two groups of plant heights of sand culture seedling, grain number per spike and mass of 1000 kernel of each kind are determined respectively, then by relativity value=low-phosphorous
The measured value of the normal phosphorus treatment of the measured value ÷ for the treatment of, calculates the relative plant height of each kind, relative grain number per spike and with respect to thousand
Weight；The relativity that each barley variety will be calculated is ranked up, and comprehensive each relativity value ranking finally filters out phase
It is phosphorus efficiency rate profit to plant height, three index value 2-4 barley varieties of synthesis highest of relative grain number per spike and relative mass of 1000 kernel
With kind, filtering out relative plant height, relative grain number per spike and 2-4 comprehensively minimum kind of three index values of relative mass of 1000 kernel is
The relatively low kind of phosphorus use efficiency is used as control；
d）By step c）The phosphorus efficiency rate for filtering out utilizes kind by step a using kind and phosphorus are inefficient）Method sprout to
The secondary screening seedling for taking root, chooses 2n plants of consistent secondary screening seedling of growing way, the secondary screening seedling of each kind is divided into two groups, with two kinds
The nutrient solution of different phosphorus concentrations carries out water planting to two groups of secondary screening seedling respectively, and culture determines two groups of water of each kind respectively after three weeks
Train seedling dry matter weight, phosphorus content, chlorophyll content, then by the normal phosphorus of measured value ÷ of relativity value=low-phosphorous treatment at
The measured value of reason, calculates the relative dry matter weight of each kind, relative phosphorus content, relative chlorophyll content；
e）Relative dry matter weight, relative phosphorus content and relative chlorophyll content according to the multiple barley varieties for calculating are carried out
Sequence, comprehensive each relativity value ranking is final to filter out relative dry matter weight, relative phosphorus content and relative chlorophyll content three
1 barley variety of individual index comprehensive highest is phosphorus use efficiency with respect to highest kind.
The step b）Middle sand culture test container use 29cm × 19cm plastic tubs, cultivation matrix by the ︰ 1 of volume ratio 1 sand
Son and vermiculite composition.
The step b）In one group of seedling pour the nutrient solution of reduced phosphorus levels, another group of seedling pours the battalion of normal phosphorus level
Nutrient solution；Poured once with nutrient solution week about, every time per basin nutrient solution 500mL, nutrient solution waters once for three days after pouring,
Water 500mL per basin every time.
The step d）In the method for water planting be that bottom seedlings nursing plate is fixed on week upward with the iron wire for scribbling antirust paint
In turnning box, transplant secondary screening seedling and be placed in nursery disk hole, add water to equal with bottom seedlings nursing plate；One of which secondary screening seedling is poured
The nutrient solution of reduced phosphorus levels, another group of secondary screening seedling pours the nutrient solution of normal phosphorus level, light application time 12h on daytime, night
12h, daytime 32 DEG C of maximum temperature, 27 DEG C of night minimum temperature；During culture daily with the hydrochloric acid that concentration is 1mol/L or
The sodium hydroxide solution of 1mol/L adjusts medium pH value to 6.5, changes one time of nutrition liquid within every two days.
The phosphorus source of the nutrient solution is KH2PO4, it is formulated with deionized water；Nitrogen in every liter of reduced phosphorus levels nutrient solution contains
It is 27mg to measure, and the nitrogen content in every liter of horizontal nutrient solution of normal phosphorus is 544mg/L, other compositions in two kinds of horizontal nutrient solutions
And content all same.Contain NH in every liter of nutrient solution4NO3It is 2.50mmol/L, K2SO4It is 2.50mmol/L, CaCl2For
2.00mmol/L 、MgSO4·7H2O is 1.50mmol/L；Trace element in the nutrient solution uses Arnon nutrient solution prescriptions：Its
Middle Fe2+2.8mg/L, is allocated into Fe-EDTA.
Screening technique of the present invention is easy to operation, substantially can objectively reflect the real conditions for absorbing phosphate fertilizer, screening
Go out excellent germplasm.The screening technique has advantages below：
1）The more screening technique that real conditions are absorbed for barley phosphate fertilizer of system at present, i.e., by three morphological indexs and
Three Selection of Physiological Indices barley phosphorus use efficiencies kind high, and the method is close with field actual production conditions, operation letter
Easy row, can carry out screening of the phosphorus efficiency rate using kind to barley variety as much as possible, so as to filter out phosphorus using effect
Rate highest barley variety.
2）It is weak for barley phosphorus efficiency rate research on utilization working foundation first, the physiology under research barley different P levels
Index, for follow-up research barley phosphorus efficiency rate absorption mechanism and breed improvement provide most basic experimental basis and experiment side
Method.
3）It is preferred that research and utilization of the different phosphate using resource：Barley P assimilation and utilization efficiency genetic background difference ten is clearly demarcated
Aobvious, the phosphorus use efficiency of preliminary screening kind high provides fine germplasm resources for barley resistance breeding.
Brief description of the drawings
Fig. 1 is the screening index column diagram of six kinds that sand culture primary dcreening operation draws in the embodiment of the present invention.
Fig. 2 is two column diagrams of the screening index of kind that secondary screening goes out in the embodiment of the present invention.
Specific embodiment
The present invention is described in detail with reference to the accompanying drawings and detailed description.
For this present situation of current barley phosphorus efficiency rate research on utilization working foundation weakness, the invention provides one kind from many
Phosphorus use efficiency is screened in individual barley variety with respect to the method for highest kind, is specifically carried out according to the following steps：
a）Obtain plant seedlings：Select full, seed size it is consistent the need for the barley variety that is screened, paving is placed in respectively
In having a culture dish of filter paper, it is placed in illumination box and sprouts to the seedling for taking root；
b）Sand culture primary dcreening operation：From step a）Each barley variety seedling in pick out 2n（n≥1）The consistent seedling of strain growth, and
The seedling of each kind is divided into two groups（N plants/group）, sand culture is carried out respectively, sand culture test container uses 29cm × 19cm plastics
Basin, cultivation matrix is made up of the sand and vermiculite of the ︰ 1 of volume ratio 1, is poured respectively using the nutrient solution of pouring method different N concentration
Two groups of seedling, the nutrient solution of different N concentration is respectively the nutrient solution of reduced phosphorus levels and the nutrient solution of normal phosphorus level, i.e., wherein
One group of nutrient solution of pouring reduced phosphorus levels（Low-phosphorous treatment）, another group of nutrient solution of the normal phosphorus level of pouring（Normal phosphorus treatment）；
Poured once with nutrient solution week about, every time per basin nutrient solution 500mL, nutrient solution waters once for three days after pouring, every time
Water 500mL per basin, until barley is ripe；
c）Two groups of plant heights of sand culture seedling, grain number per spike and mass of 1000 kernel of each kind are determined respectively, then by relativity value=low-phosphorous
The measured value of the normal phosphorus treatment of the measured value ÷ for the treatment of, calculates the relative plant height of each kind, relative grain number per spike and with respect to thousand
Weight（Please determine whether this section words are correct）；The relativity that each barley variety will be calculated is ranked up, comprehensive each relative
Character value ranking finally filters out relative plant height, three index value synthesis highest 2-4 of relative grain number per spike and relative mass of 1000 kernel
Individual barley variety utilizes kind for phosphorus efficiency rate, filters out relative plant height, three index numbers of relative grain number per spike and relative mass of 1000 kernel
The comprehensive 2-4 minimum kind of value is the relatively low kind of phosphorus use efficiency as control；
d）Water planting secondary screening：By step c）The phosphorus efficiency rate for filtering out utilizes kind by step a using kind and phosphorus are inefficient）Side
Method is sprouted to the secondary screening seedling for taking root, and chooses the consistent 2n of growing way（n≥1）Strain secondary screening seedling, by the secondary screening seedling of each kind
It is divided into two groups（N plants/group）, two groups of secondary screening seedling are carried out into water planting in plastic box respectively, Turnover Box external diameter is 510 ×
, upward be fixed on Turnover Box bottom seedlings nursing plate with the iron wire for scribbling antirust paint by 390 × 150mm, is transplanted seedling and is placed in nursery
In disk hole, add water to equal with bottom seedlings nursing plate.One of which pours the nutrient solution of reduced phosphorus levels（Low-phosphorous treatment）, another group is poured
Fill the nutrient solution of normal phosphorus level（Normal phosphorus treatment）, light application time 12h on daytime, night 12h, daytime 32 DEG C of maximum temperature, night
Between 27 DEG C of minimum temperature.Training is adjusted during culture with the sodium hydroxide solution of the hydrochloric acid or 1mol/L that concentration is 1mol/L daily
Nutrient solution pH value changes one time of nutrition liquid in every two days to 6.5；Solution culture method determines two groups of Rooted Cuttings of each kind respectively after three weeks
Dry matter weight, phosphorus content, chlorophyll content, then processed by the normal phosphorus of measured value ÷ of relativity value=low-phosphorous treatment
Measured value, calculates the relative dry matter weight of each kind, relative phosphorus content, relative chlorophyll content；
e）Relative dry matter weight, relative phosphorus content and relative chlorophyll content according to the multiple barley varieties for calculating are carried out
Sequence, comprehensive each relativity value ranking is final to filter out relative dry matter weight, relative phosphorus content and relative chlorophyll content three
1 barley variety of individual index comprehensive highest is phosphorus use efficiency with respect to highest kind.
Filter out 1 minimum with respect to dry matter weight, relatively phosphorus content and three index comprehensives of relative chlorophyll content big
Wheat variety is the relatively minimum kind of phosphorus use efficiency as control.The phosphorus use efficiency for filtering out is with respect to highest kind and phosphorus
The relatively minimum kind of utilization ratio can carry out subsequent physiological mechanism experiment and molecular mechanism experiment.
The phosphorus source of nutrient solution is KH in screening technique of the present invention2PO4, it is formulated with deionized water.Every liter of reduced phosphorus levels battalion
Phosphorus content in nutrient solution is 27mg, and the nitrogen content in every liter of horizontal nutrient solution of normal phosphorus is 544mg；In two kinds of horizontal nutrient solutions
Other compositions and content all same.
The concentration of nitrogen, potassium, calcium, magnesium is shown in Table 1, pH 6.5 using the Xiu Yite Solution culture methods of improvement in nutrient solution.
The Xiu Yite nutrient solution prescriptions of the improvement of table 1
Trace element in nutrient solution uses Arnon nutrient solution prescriptions, is shown in Table 2, wherein Fe2+ 2.8mg/L, is matched somebody with somebody with Fe-EDTA
Enter.
The Arnon microelement nutritious liquids of table 2 are formulated
The measure of screening index in the inventive method：
Screening index is the relative value between control variety：With respect to plant height, relative grain number per spike, relative mass of 1000 kernel, relative dry matter weight,
With respect to phosphorus content and relative chlorophyll content.It is comprehensive for the survey of sand culture primary dcreening operation with respect to plant height, relative grain number per spike and relative mass of 1000 kernel
Determine index, be comprehensively used for water planting secondary screening with respect to dry matter weight, relative phosphorus content and relative chlorophyll content, with reference to screening knot twice
Fruit judges the height of barley variety phosphorus use efficiency.
Plant height is determined：After barley maturation, 6 plants of plant of each kind difference phosphorus concentration treatment are randomly selected, measured with ruler
Barley Roots basal part of stem arrives the distance between top, calculates its average value.
Grain number per spike is determined：After results, 6, the tassel of each kind difference phosphorus concentration treatment is randomly selected, ask it to put down after counting
Average.
Mass of 1000 kernel determines：After threshing, 1000 seeds of each kind difference phosphorus concentration treatment are randomly selected, electronic balance claims
Its average value is sought after weight.
Plant weights are determined：Then fresh sample is first cooled to 80 DEG C in 105 DEG C of de-enzyme 30min, by most moisture, is finally claimed
Weight.
Measuring chlorophyll content：Fresh blade is taken, tissue surface dirt is cleaned, shredded（Remove middle arteries）, mix；Weigh and cut
Broken fresh sample 0.2g, is respectively put into mortar totally by 3 parts, plus a small amount of quartz sand, Paris white and 2-3mL mass percents
Concentration is 95% ethanol, is ground into homogenate, then adds ethanol 10mL, continues to be ground to tissue turned pale.Stand 3-5min；Filter paper 1 is taken,
Put in funnel, with ethanol wet, extract solution is poured into funnel along glass bar, be filled into 25mL brown volumetric flasks, with a small amount of
Alcohol flushing mortar, pestle and residue for several times, are finally poured into funnel together with residue；Ethanol is drawn with dropper, by filter paper
On chloroplast pigment all wash volumetric flask.Untill redgreen in filter paper and residue.Finally it is settled to ethanol
25mL, shakes up；Chloroplast pigment extract solution is poured into the cuvette of optical path 1cm, is blank with 95% ethanol, in wavelength 663nm
With mensuration absorbance under 645nm.
Full phosphorus is determined：By Plant samples dry it is levigate after, with the full phosphorus of overground part of vanadium molybdenum Huang colorimetric method for determining each sample
Content.
On the screening index of phosphorus use efficiency kind high in the present invention, written standards are there is no at present.The present invention is based on
Relative plant height of the plant after maturation, relative grain number per spike, relative mass of 1000 kernel carry out primary dcreening operation, then relatively dry in seedling stage for it
Material weight, relative phosphorus content, relative chlorophyll content carry out secondary screening, by the combination of time of infertility sand culture and seedling stage water planting, visitor
The real conditions that ground reflection barley absorbs phosphate fertilizer are seen, the screening side of reliable, the easy phosphorus use efficiency of barley kind high is set up
Method, filters out excellent germplasm.
The principle of screening technique of the present invention：One of the performance symptom of crop under the conditions of phosphorus is lacked is exactly slow-growing, biology
Amount decreases.High efficiency kind has larger biological accumulation amount, and inefficient kind is then because phosphorus supplies insufficient, and biology is long-pending
Tired amount very little.The difference of different cultivars Parameters of Barley Seedling Dry matter weight of shoot can react its suction to nutrient to a certain extent
The difference of receipts, transhipment and utilization ratio.And, phosphorus all plays an important role to the tiller of plant, branch and root growth, and
Promote synthesis, conversion and the transport of carbohydrate, the growth to seed is favourable.Plant lack phosphorus when, can influence cell growth and
Division, reduces tiller branch, and spire growth retardation, plant vivo protein synthesis declines.And lacking phosphorus can cause sugared operating
It is obstructed, the content of sugar is relative in nutrition organs improves, promotes the formation of anthocyanidin so that the leaf of plant is in abnormal dark
Green or aubergine.Phosphorus Absorption in Plants total amount, i.e. absorbed phosphorus by crop amount, are to represent that plant inhales the index of phosphorus capacity of water.Low-phosphorous
Under stress, different genotype plant phosphorus absorb total amount decline.But high efficiency kind remains to absorb under relatively low phosphorus level
The need for more phosphorus grows meeting it, so relative ratio is higher.
Embodiment
1st, plant seedlings are obtained
From 16 barley varieties that needs are screened（It is shown in Table 3）In select full, seed size it is consistent barley variety difference
It is placed in the culture dish for being covered with filter paper, is placed in illumination box and sprouts to the seedling for taking root, growing way is chosen after 5 days consistent
Seedling as test process material.Light application time is 12h on daytime, night 12h, and daytime, maximum temperature was 32 DEG C, and night is minimum
Temperature is 27 DEG C.
The different barley variety numberings of table 3
2nd, sand culture primary dcreening operation
Choosing 12 plants of consistent seedling of growing way from the plant seedlings of each kind carries out sand culture test.Sand culture test container is used
29 × 19cm plastic tubs, cultivation matrix is made up of the sand and vermiculite that volume ratio is 1 ︰ 1.With deionized water and KH2PO4Prepare phosphorus
Content is the nutrient solution of 27mg/L reduced phosphorus levels, with deionized water and KH2PO4It is the normal phosphorus level of 544mg/L to prepare phosphorus content
Nutrient solution.The pH value of two kinds of horizontal nutrient solutions is 6.5；Nitrogen in two kinds of horizontal nutrient solutions, potassium, calcium, magnesium concentration it is equal
It is identical, i.e. NH4NO3It is 2.50mmol/L, K2SO4It is 2.50mmol/L, CaCl2It is 2.00mmol/L, MgSO4·7H2O is
1.50mmol/L；Other trace elements use Arnon nutrient solution prescriptions, i.e. H in nutrient solution3BO3It is 2.86g/L, MnCl2·
4H2O is 1.81g/L, ZnSO4·7H2O is 0.22g/L, CuSO4·5H2O is 0.08g/L, H2MoO4·4H2O is 0.02g/L.Battalion
Fe in nutrient solution2+ 2.8mg/L, is allocated into Fe-EDTA.
The seedling of each kind is divided into two groups（6 plants/group）, one group of nutrient solution with phosphorus content 544mg/L is week about
Pour once, another group of nutrient solution with phosphorus content 27mg/L is poured once week about, every time per basin nutrient solution
500mL, nutrient solution waters once for three days after pouring, and water 500mL per basin every time, until barley is ripe.Each kind, determines respectively
Every plant of plant height of barley, grain number per spike, mass of 1000 kernel in 6 plants of barleys in low-phosphorous treatment group, then calculate its average plant height, average respectively
Grain number per spike, average mass of 1000 kernel as low-phosphorous treatment measured value；Using same method draw normal phosphorus treatment group average plant height,
The measured value that average grain number per spike, average mass of 1000 kernel are processed as normal phosphorus.Then by the measured value ÷ of relativity=low-phosphorous treatment
The measured value of normal phosphorus treatment, calculates the screening index of each kind：With respect to plant height, relative grain number per spike and relative mass of 1000 kernel.Often
Screening index obtained by individual kind is shown in Table 4.
Table 4 is 16 screening index of barley variety obtained by sand culture primary dcreening operation
Comprehensive Correlation is carried out from the screening index of the multiple barley varieties for being drawn, with respect to plant height：5#, 15#, 8# numerical value are minimum,
7#, 2#, 11# numerical value highest；With respect to grain number per spike：5#, 15#, 8# numerical value are minimum, 7#, 3#, 11# numerical value highest；With respect to mass of 1000 kernel：
5#, 15#, 8# numerical value are minimum, 7#, 2#, 11# numerical value highest.Comprehensive indices, filter out three index comprehensive numerical value highests
3 kinds 7#, 2#, 11# are the of a relatively high kind of phosphorus use efficiency, minimum 3 of the three index comprehensive numerical value for filtering out
Kind 5#, 15#, 8# are the relatively low kind of phosphorus use efficiency as control, 6 cylindricalitys of the screening index of kind for filtering out
Figure, as shown in figure 1, as can be seen from the figure phosphorus use efficiency kind high in relative plant height, relative grain number per spike and with respect to thousand
The low kind of phosphorus use efficiency is above on again.
By by the kind higher of 3 phosphorus use efficiencies selected by sand culture primary dcreening operation（7#、2#、11#）With phosphorus use efficiency compared with
3 low kinds（5#、15#、8#）Repeat water planting and obtain Rooted Cuttings, be divided to two groups to enter field headquarters the plant seedlings of each kind
Nutrient solution culture, the nutrient solution of one of which reduced phosphorus levels（Low-phosphorous treatment）, another group of Solution culture method with normal phosphorus level
（Normal phosphorus treatment）；Solution culture method container is plastic box.During culture daily with the hydrochloric acid that concentration is 1mol/L or
The sodium hydroxide solution of 1mol/L adjusts pH value to 6.5, changes one time of nutrition liquid within every two days.After barley grows 3 weeks in greenhouse
Determine its indices.The formula of nutrient solution wherein used and the assay method of screening index are as previously described.Determine respectively every
The screening index of individual kind, the screening index of the barley variety that will be drawn carries out Comprehensive Correlation, filters out three index comprehensives
1 kind of numerical value highest is the phosphorus use efficiency highest kind in 16 barley varieties（7#）, filter out three indexs comprehensive
It is the minimum kind of phosphorus use efficiency in 16 barley varieties to close 1 minimum kind of numerical value（5#）As control.Fig. 2 is battalion
Phosphorus efficiency rate is filtered out in nutrient solution culture secondary screening using kind 7# and the inefficient column diagram using kind 5# screening index of phosphorus.From
Fig. 2 can be seen that phosphorus efficiency rate is substantially better than phosphorus poor efficiency and utilizes kind using kind in three indexs.
The phosphorus efficiency for filtering out carries out next step experiment using kind 7# and phosphorus are poorly efficient using kind 5#（Physiological Mechanism and point
Handset is managed）.
Claims (6)
- It is 1. a kind of that method of the phosphorus efficiency rate using kind is screened from multiple barley varieties, it is characterised in that：The screening technique has Body is carried out according to the following steps：a）Select full, seed size it is consistent the need for the barley variety that is screened, the culture for being covered with filter paper is placed in respectively In ware, sprouted in illumination box to the seedling for taking root；b）Sand culture primary dcreening operation：From step a）Each barley variety seedling in pick out the 2n plants of consistent seedling of growth, and by each The seedling of kind is divided into two groups, two groups of seedling is poured respectively with the nutrient solution of two kinds of different phosphorus concentrations, until barley is ripe；c）Two groups of plant heights of sand culture seedling, grain number per spike and mass of 1000 kernel of each kind are determined respectively, then by relativity value=low-phosphorous The measured value of the normal phosphorus treatment of the measured value ÷ for the treatment of, calculates the relative plant height of each kind, relative grain number per spike and with respect to thousand Weight；The relativity that each barley variety will be calculated is ranked up, and comprehensive each relativity value ranking finally filters out phase It is phosphorus efficiency rate profit to plant height, three index value 2-4 barley varieties of synthesis highest of relative grain number per spike and relative mass of 1000 kernel With kind, filtering out relative plant height, relative grain number per spike and 2-4 comprehensively minimum kind of three index values of relative mass of 1000 kernel is The relatively low kind of phosphorus use efficiency is used as control；d）By step c）The phosphorus efficiency rate for filtering out utilizes kind by step a using kind and phosphorus are inefficient）Method sprout to The secondary screening seedling for taking root, chooses 2n plants of consistent secondary screening seedling of growing way, the secondary screening seedling of each kind is divided into two groups, with two kinds The nutrient solution of different phosphorus concentrations carries out water planting to two groups of secondary screening seedling respectively, and culture determines two groups of water of each kind respectively after three weeks Train seedling dry matter weight, phosphorus content, chlorophyll content, then by the normal phosphorus of measured value ÷ of relativity value=low-phosphorous treatment at The measured value of reason, calculates the relative dry matter weight of each kind, relative phosphorus content, relative chlorophyll content；e）Relative dry matter weight, relative phosphorus content and relative chlorophyll content according to the multiple barley varieties for calculating are carried out Sequence, comprehensive each relativity value ranking is final to filter out relative dry matter weight, relative phosphorus content and relative chlorophyll content three 1 barley variety of individual index comprehensive highest is phosphorus use efficiency with respect to highest kind.
- 2. according to claim 1 that method of the phosphorus efficiency rate using kind is screened from multiple barley varieties, its feature exists In：The step b）Middle sand culture test container uses 29cm × 19cm plastic tubs, sand and leech of the cultivation matrix by the ︰ 1 of volume ratio 1 Stone is constituted.
- 3. according to claim 1 that method of the phosphorus efficiency rate using kind is screened from multiple barley varieties, its feature exists In：The step b）In one group of seedling pour the nutrient solution of reduced phosphorus levels, another group of seedling pours the nutrient solution of normal phosphorus level； Poured once with nutrient solution week about, every time per basin nutrient solution 500mL, nutrient solution waters once for three days after pouring, every time Water 500mL per basin.
- 4. according to claim 1 that method of the phosphorus efficiency rate using kind is screened from multiple barley varieties, its feature exists In：The step d）In the method for water planting be that bottom seedlings nursing plate is fixed on Turnover Box upward with the iron wire for scribbling antirust paint On, transplant secondary screening seedling and be placed in nursery disk hole, add water to equal with bottom seedlings nursing plate；One of which secondary screening seedling is poured low-phosphorous The nutrient solution of level, another group of nutrient solution of the secondary screening seedling normal phosphorus level of pouring, light application time 12h on daytime, night 12h, in vain 32 DEG C of its maximum temperature, 27 DEG C of night minimum temperature；Daily with the hydrochloric acid or the hydrogen of 1mol/L that concentration is 1mol/L during culture Sodium hydroxide solution adjusts medium pH value to 6.5, changes one time of nutrition liquid within every two days.
- 5. method of the phosphorus efficiency rate using kind, its feature are screened from multiple barley varieties according to claim 3 or 4 It is：The phosphorus source of the nutrient solution is KH2PO4, it is formulated with deionized water；Nitrogen content in every liter of reduced phosphorus levels nutrient solution Be 27mg, the nitrogen content in every liter of horizontal nutrient solution of normal phosphorus is 544mg/L, other compositions in two kinds of horizontal nutrient solutions and Content all same.
- 6. according to claim 5 that method of the phosphorus efficiency rate using kind is screened from multiple barley varieties, its feature exists In：Contain NH in every liter of nutrient solution4NO3It is 2.50mmol/L, K2SO4It is 2.50mmol/L, CaCl2For 2.00mmol/L, MgSO4·7H2O is 1.50mmol/L；Trace element in the nutrient solution uses Arnon nutrient solution prescriptions：Wherein Fe2+ 2.8mg/L, is allocated into Fe-EDTA.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107746895A (en) * | 2017-11-10 | 2018-03-02 | 四川农业大学 | It is a kind of it is low-phosphorous under the conditions of lifted barley harvest index QTL site molecular labeling and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107746895A (en) * | 2017-11-10 | 2018-03-02 | 四川农业大学 | It is a kind of it is low-phosphorous under the conditions of lifted barley harvest index QTL site molecular labeling and application |
| CN107746895B (en) * | 2017-11-10 | 2021-02-02 | 四川农业大学 | Molecular marker for improving barley harvest index QTL site under low-phosphorus condition and application |
| CN107853154A (en) * | 2017-11-24 | 2018-03-30 | 扬州大学 | A kind of method for screening Tolerant to low P rice varieties |
| CN110150115A (en) * | 2019-04-22 | 2019-08-23 | 河南省农业科学院芝麻研究中心 | Screen mill water culture nutrient solution and its application of safflower Tolerant to low P germplasm |
| CN111631093A (en) * | 2020-06-11 | 2020-09-08 | 江苏省农业科学院泰州农科所 | Efficient cultivation method for mallotus japonicus |
| CN116034869A (en) * | 2022-12-13 | 2023-05-02 | 河南农业大学 | Method for rapidly screening wheat varieties with different phosphorus efficiencies |
| CN116034869B (en) * | 2022-12-13 | 2024-03-15 | 河南农业大学 | Method for rapidly screening wheat varieties with different phosphorus efficiencies |
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