CN106706382B - Soil springtail collecting device and application thereof - Google Patents
Soil springtail collecting device and application thereof Download PDFInfo
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- CN106706382B CN106706382B CN201611078512.5A CN201611078512A CN106706382B CN 106706382 B CN106706382 B CN 106706382B CN 201611078512 A CN201611078512 A CN 201611078512A CN 106706382 B CN106706382 B CN 106706382B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The soil springtail collecting device and the application thereof sequentially comprise a cover provided with a fan, an upper device, a lower device and a collecting bottle from top to bottom; the upper device and the lower device are connected through a rectangular sheet and a screw which are arranged at the connecting part; a knocking device is arranged on one side of the rectangular sheet, which is positioned in the cavity of the lower device, an eccentric motor is arranged on the outer side of the top end of the lower device, a steel screen a is connected to the shaft of the eccentric motor, the steel wire a penetrates through the inner wall of the lower device to be connected with the steel screen, and the knocking device and the steel screen are positioned at the same horizontal height; the lower part in the lower device cavity is provided with two collecting channels, and the collecting bottle is arranged below the collecting channels. At present, no experimental device capable of improving the purity of a springtail sample and collecting soil springtails exists, and the invention solves the problems that the springtail sample is impure, the collecting efficiency is low, the time consumption is long, and large errors are easy to cause in the measurement of the index with large variability in the springtails in the current springtail collecting method.
Description
Technical Field
The invention relates to a soil animal collecting device and application thereof, in particular to a soil springtail collecting device and application thereof.
Background
With the deterioration of the earth environment, people pay more attention to environmental problems, and the accumulation of toxic and harmful substances in the soil affects the stability and sustainability of the soil ecosystem. In recent 20 years, ecological risk assessment is gradually rising, and the process of exposing to one or more stress factors to cause adverse ecological effects is comprehensively evaluated by using science and technology such as mathematics, computers and the like on the basis of ecology, toxicology, biology and chemistry as theoretical basis. Terrestrial invertebrates are widely distributed in soil and are an important component of the soil ecosystem. In practical risk assessment it is necessary to select the appropriate sensitive species depending on the sensitivity and tolerance of the species to the contaminant and the end point of the evaluation. The springtails are sensitive to soil pollution, and a certain research basis exists for evaluating the ecological risk of soil pollution through an ecological toxicity test of the springtails, but the size of the springtails is small, for example, the body length of white-character springtails of 9-12d is less than 1mm. The current method of collecting the springtails is to suspend the springtails on the water surface using a waxy layer on the surface of the springtails, and then scoop the springtails into another container with a scoop until all the springtails are transferred to the other container. The method is used for collecting the tested springtail samples, and has a plurality of defects, for example, when water floats, a plurality of foams are arranged on the water surface to submerge the springtail, organic matters in soil are suspended on the water surface, so that the springtail samples are impure, the collecting efficiency is extremely low, 4 samples/(about 250 samples per sample) are quicker, the time consumption is long, and large errors are easy to be caused for measuring the index with large variability in the springtail. Therefore, on the premise of not affecting the measurement of various indexes of the springtail sample and the purity of the sample, the improvement of the collection efficiency of the springtail is particularly important, however, no experimental device capable of improving the purity of the springtail sample and collecting soil springtails is available at present.
Disclosure of Invention
The technical problems to be solved are as follows: the existing method for collecting the springtails has the defects of impure collected springtails, low collection efficiency, long time consumption and easy large error in measurement of indexes with large variability in the springtails, and no experimental device capable of improving the purity of the springtails and collecting soil springtails exists at present.
Aiming at the technical problems, the invention provides a soil springtail collecting device and application thereof.
The technical scheme is as follows: the soil springtail collecting device sequentially comprises a cover provided with a fan, an upper device, a lower device and a collecting bottle from top to bottom;
the cover provided with the fan is arranged on the upper device and is connected with the upper device through threads;
the upper device and the lower device are connected through a rectangular sheet and a screw which are arranged at the connecting part;
a knocking device is arranged on one side of the rectangular sheet, which is positioned in the cavity of the lower device, an eccentric motor is arranged on the outer side of the top end of the lower device, a steel wire a is connected to the shaft of the eccentric motor, the steel wire a penetrates through the inner wall of the lower device and is connected with a steel screen, and the knocking device and the steel screen are positioned at the same horizontal height;
the lower part in the lower part device cavity is respectively equipped with an inclined plane by cavity center department about, inclined plane and lower part device's lateral wall form two collecting channel, and the collecting bottle is located the below of collecting channel, and the top junction of two inclined planes is equipped with the baffle, the baffle is about the junction as the axle.
Preferably, gauze is arranged on the cover provided with the fan and positioned at the air inlet end of the fan.
Preferably, the upper part and the upper part of the lower part device are both cylinders, the materials are water repellent materials, the upper part device and the lower part device extend out of at least two rectangular thin sheets which are symmetrical in the center of the cylinders and have the length of 2cm and the width of 1cm along the radial direction at the connecting position, and concentric holes are formed in the corresponding rectangular thin sheets on the upper part device and the lower part device.
Preferably, the knocking device is a rectangular steel sheet, and one side of the steel sheet is provided with a section of steel wire b.
Preferably, the eccentric motor is connected with the steel screen through a steel wire a, a cross-shaped groove is formed in the side face of the steel screen, and one end, connected with the steel screen, of the steel wire a is T-shaped and is clamped in the cross-shaped groove of the steel screen.
Preferably, the partition board leans against the side wall of the lower device and is in sealing contact with the side wall, and the partition board is made of water-repellent material.
Preferably, the upper and lower devices are colorless and transparent, and the steel screen and separator are dark in color, in contrast to white.
The application of the device in collecting soil springtails comprises the following steps:
1) The upper device and the lower device are connected through a rectangular sheet and a screw which are arranged at the joint, and the left and right sides of the lower device are inserted with a collecting bottle;
2) Pulling a baffle plate in a lower device to the right, adding water into a sample to be collected of the springtails, pouring the sample into the lower device at the center of the steel screen at the upper device until all the springtails are poured into the steel screen, if the springtails are in the cup body, continuously adding water into the cup body, and continuously pouring the sample into the center of the steel screen after the springtails float on the water surface until all the springtails are poured onto the steel screen;
3) After entering a collecting bottle for collecting moisture along a channel for collecting moisture, the partition plate is pulled to the left side, and the steel screen is turned over;
4) After the cover provided with the fan is covered, the eccentric motor is opened, so that the steel screen shakes left and right, the side edge is used for impacting the small steel sheet in the knocking device, and meanwhile, the fan on the cover is opened, so that moisture is evaporated, and thus, the springtail falls off the steel screen and enters the collecting bottle along the collecting channel.
The beneficial effects are that: 1. according to the invention, the steel screen is adopted to filter water to leave the springtail, then the steel screen is turned over, the steel screen swings left and right and back and collides with a small steel sheet in the knocking device under the action of the eccentric motor, cold air is generated by a fan on the cover, evaporation of water is increased, the speed of the springtail falling off the steel screen is increased, the springtail falls off the steel screen and enters the collecting channel, so that the efficiency of collecting the springtail is improved.
2. The invention adopts the upper and lower parts which are separated, is convenient for cleaning the inside, and is connected with the screw through the rectangular thin sheet arranged at the joint, thereby being convenient for disassembly and installation. And the knocking device can be disassembled, so that the cleaning and the replacement are convenient.
3. The lower device is provided with the collecting channel, the channel for collecting moisture and the channel for collecting the springtails can be formed independently by pulling the partition plate capable of swinging left and right, the moisture and the springtails can be separated conveniently, and the inclined plane in the collecting channel has a certain gradient, so that the collection of the springtails can be accelerated.
4. The material adopts transparent water repellent material, so that water can be less adhered to the surface of the device; the color of the steel screen and the color of the partition plate are dark, and the other device colors are transparent, so that whether the screen and the partition plate have springtails or not can be observed, and the whole collecting process can be watched through the device.
The invention can ensure the purity of the collected springtail sample, improve the efficiency of collecting the springtail, meet the measurement of each index in the springtail sample and reduce experimental errors.
Drawings
Fig. 1 is a schematic structural view of a springtail collecting device;
fig. 2 is a schematic structural view of a cover provided with a fan in the springtail collecting device.
In the figure: 1. a cover provided with a fan; 2. an upper device; 3. a steel screen; 4. a knocking device; 5. an eccentric motor; 6. a partition plate; 7. a lower device; 8. a collection channel; 9. a collection bottle; 10. a fan; 11. gauze.
Detailed Description
The invention will be described in further detail with reference to the accompanying drawings and detailed description.
Example 1
As shown in fig. 1, the soil springtail collecting device comprises a cover 1 with a fan, an upper device 2, a rectangular sheet arranged at a joint, and an upper device 2 and a lower device 7 connected by screws, wherein a knocking device 4 is arranged in a cavity of the lower device 7 on the inner side of the rectangular sheet, the knocking device 4 is a rectangular steel sheet, and one side of the steel sheet is provided with a section of steel wire b; an eccentric motor 5 is arranged on the outer side of the top end of the lower device 7, a steel wire a is connected to the shaft of the eccentric motor 5, the steel wire a penetrates through the inner wall of the lower device 7 and is connected with the steel screen 3, and the knocking device 4 and the steel screen 3 are positioned at the same horizontal height; the lower end in the cavity of the lower device 7 is respectively provided with an inclined plane from the left to the right at the center, the inclined planes and the side wall of the lower device 7 form two collecting channels 8, a collecting bottle 9 is arranged below the collecting channels 8, a partition plate 6 is arranged at the intersection of the two inclined planes, and the partition plate 6 swings left and right by taking the intersection as an axis.
A gauze 11 is arranged on the cover 1 provided with the fan and positioned at the air inlet end of the fan 10 so as to prevent the excessive wind speed; the cover 1 provided with the fan is internally provided with threads and is in threaded rotation connection with the upper device 2.
The upper part of the lower device 7 and the upper device 2 are both cylinders and made of transparent water repellent materials with the thickness of 3mm, the upper device 2 and the lower device 7 extend out of at least two rectangular thin sheets with the length of 2cm, the width of 1cm and the thickness of 1mm which are symmetrical by the center of the cylinders respectively along the radial direction at the joint, and concentric holes are arranged on the corresponding rectangular thin sheets on the upper device 2 and the lower device 7 so as to be convenient for inserting screws. The top of the outside of the upper device 2 is threaded for attachment to a cap 1 provided with a fan.
Inside the cavity of the lower device 7, the ends of the rectangular sheet grooves are respectively provided with a hollow semi-cylinder perpendicular to the sheet section, the hollow semi-cylinder is connected with the lower device 7, the knocking device 4 is inserted into the rectangular sheet from the hollow position of the hollow semi-cylinder, the rectangular sheet connecting surface is radially provided with a groove, and the hollow semi-cylinder is used for fixing the knocking device 4.
The shaft of the eccentric motor 5 is connected with a steel wire a, the steel wire a penetrates through the inner wall of the lower device 7 and is connected with the steel screen, and the tail end of the steel wire a is T-shaped and can be firmly clamped with a cross-shaped groove on the side surface of the steel screen 3. The steel screen 3 is a 300-mesh steel screen, the diameter of the steel screen is smaller than the inner diameter of a cylinder at the upper half part of the lower device 7, the steel screen is positioned at the top end of the lower device 7, is horizontal to the knocking device 4 and is provided with a gap, and the steel screen is black in color.
The lower end in the cavity of the lower device 7 is respectively provided with an inclined plane from the left to the right at the center, the inclined planes and the side wall of the lower device 7 form two collecting channels 8, a collecting bottle 9 is arranged below the collecting channels 8, a partition plate 6 is arranged at the top end junction of the two inclined planes, and the partition plate 6 swings left and right by taking the junction as an axis. The partition board leans against the side wall of the lower device 7, a groove is engraved on the side wall contacted with the partition board, the partition board 6 can be in sealing contact with the side wall, the partition board 6 is made of water-repellent material, and the color is dark color which is obviously contrasted with white, such as black. The application specific steps of the invention are as follows: the upper device 2 and the lower device 7 are connected through a rectangular sheet and a screw which are arranged at the joint, and the collecting bottle 9 is inserted into the left side and the right side of the lower device 7; pulling a partition plate 6 in a lower device 7 to the right, adding water into a sample to be collected of the springtails, pouring the sample into the lower device 7 at the position of the upper device 2 along the center of the steel screen 3 until all the springtails are poured into the steel screen 3, if the springtails are in the cup body, continuing to add water into the cup body, and continuing to pour the springtails at the center of the steel screen 3 after the springtails float on the water surface until all the springtails are poured onto the steel screen 3; after entering the collecting bottle along the channel for collecting the moisture, the partition plate 6 is pulled to the left side, and the steel screen 3 is turned over; after the cover provided with the fan is covered, the eccentric motor 5 is opened, so that the steel screen 3 shakes left and right, the side edge is used for striking the small steel sheet in the knocking device 4, and meanwhile, the fan 10 on the cover is opened, so that the moisture is evaporated, and the springtail falls off the steel screen 3 rapidly and enters the collecting bottle along the collecting channel. The collecting bottle 11 is transparent in color and made of water-repellent materials.
Example 2
Using the apparatus of example 1, the device was used to treat 20 day old springtails 20, 50, 100, 200, 300 heads in dishes (specification: 5cm high, 5cm diameter) with a vacuum fluke, 3 replicates were set for each treatment, deionized water was added thereto, then poured along the upper apparatus 2 into the center of the steel screen 3 until the springtails in the dishes were all poured into the steel screen 3, then the partition 6 was pulled, the lid 1 provided with the fan was covered, the steel screen 3 was turned over, the fan 10 and eccentric motor 5 were turned on for half a minute, then the fan 10 and eccentric motor 5 were turned off, and then the springtails in the springtail collection bottles were poured into dishes containing deionized water, and 2-3 drops of blue black ink were dropped, and in combination with the camera D90 and software image J, the liquid surface in the dishes was photographed first, and then the photographs were counted using the software image J, with the results shown in the following table.
Table 1 recovery rate of the springtail collector
Note that: t1, T2, T3, T4, T5 denote the addition of the heads 20, 50, 100, 200, 300 respectively to the dishes.
The results are shown in Table 1, and the recovery rate of the springtails is over 99 percent by combining software counting analysis, so that the purpose of collecting the springtails can be achieved.
The invention adopts transparent water repellent material, which not only can see the whole collecting process, but also can reduce the water adhered to the inner wall of the device as much as possible.
The invention can ensure the purity of the collected springtail sample, improve the efficiency of collecting the springtail, meet the measurement of each index in the springtail sample and reduce experimental errors.
The foregoing is merely illustrative of specific embodiments of the present invention, and the scope of the invention is not limited thereto, but any changes or substitutions that do not undergo the inventive effort should be construed as falling within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.
Claims (7)
1. Soil springtail collection device, its characterized in that: the soil springtail collecting device sequentially comprises a cover (1) provided with a fan, an upper device (2), a lower device (7) and a collecting bottle (9) from top to bottom;
the cover (1) provided with the fan is arranged on the upper device (2) and is connected with the upper device through threads;
the upper device (2) is connected with the lower device (7) through a rectangular sheet and a screw which are arranged at the joint;
the device is characterized in that a knocking device (4) is arranged on one side of the rectangular sheet, which is positioned in a cavity of the lower device (7), an eccentric motor (5) is arranged on the outer side of the top end of the lower device (7), a steel wire a is connected to a shaft of the eccentric motor (5), the steel wire a penetrates through the inner wall of the lower device (7) to be connected with a steel screen (3), and the knocking device (4) and the steel screen (3) are positioned at the same horizontal height;
the utility model discloses a device for collecting water, including lower device (7), upper device (7), baffle (6), upper device (2) and lower device (7), lower part in lower device (7) cavity is controlled by cavity center department and is respectively equipped with an inclined plane, inclined plane and the lateral wall of lower device (7) form two collecting channel (8), and collecting bottle (9) are located the below of collecting channel (8), and the top junction of two inclined planes is equipped with baffle (6), baffle (6) are used the junction to swing about being equipped with gauze (11) with the junction, be located fan (10) air inlet end on lid (1) that is equipped with the fan, upper portion and upper device (2) of lower device (7) are the cylinder, and the material is water repellent material, upper device (2) and lower device (7) are along the junction radial, respectively outside stretches out two long 2cm with cylinder central symmetry, wide 1 cm's rectangle thin slice on upper device (2) and lower device (7) are last corresponding rectangle thin slice all to be equipped with concentric hole.
2. The soil hopper collecting device as recited in claim 1, wherein: the knocking device (4) is a rectangular steel sheet, and one side of the steel sheet is provided with a section of steel wire b.
3. The soil hopper collecting device as recited in claim 1, wherein: the eccentric motor (5) is connected with the steel screen (3) through a steel wire a, a cross-shaped groove is formed in the side face of the steel screen (3), and one end, connected with the steel screen (3), of the steel wire a is T-shaped and is clamped in the cross-shaped groove of the steel screen (3).
4. The soil hopper collecting device as recited in claim 1, wherein: the partition board (6) leans against the side wall of the lower device (7) and is in sealing contact with the side wall, and the partition board (6) is made of water-repellent materials.
5. The soil hopper collecting device as recited in claim 1, wherein: the upper device (2) and the lower device (7) are colorless and transparent, and the colors of the steel screen (3) and the partition plate (6) are dark colors which are obviously compared with white colors.
6. Use of a device according to claim 1 for collecting soil springtails.
7. The use according to claim 6, characterized in that:
1) The upper device (2) and the lower device (7) are connected through a rectangular sheet and a screw which are arranged at the joint, and a collecting bottle (9) is inserted into the left side and the right side of the lower device (7);
2) Pulling a baffle plate (6) in a lower device (7) to the right, adding water into a sample to be collected of the springtails, pouring the sample into the lower device (7) at the center of the upper device (2) along the steel screen (3) until all the springtails are poured into the steel screen (3), and if the springtails are in the cup body, continuing to add water into the cup body, so that the springtails float on the water surface and then continue to be poured into the center of the steel screen (3) until all the springtails are poured onto the steel screen (3);
3) After entering a collecting bottle for collecting moisture along a channel for collecting moisture, the partition plate (6) is pulled to the left side, and the steel screen (3) is turned over;
4) After the cover (1) with the fan is covered, the eccentric motor (5) is opened, the steel screen (3) shakes left and right, the side edge is used for striking the small steel sheet in the knocking device (4), and meanwhile, the fan (10) on the cover is opened, so that water is evaporated, and the springtail falls off the steel screen (3) and enters the collecting bottle along the collecting channel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611078512.5A CN106706382B (en) | 2016-11-29 | 2016-11-29 | Soil springtail collecting device and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611078512.5A CN106706382B (en) | 2016-11-29 | 2016-11-29 | Soil springtail collecting device and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106706382A CN106706382A (en) | 2017-05-24 |
| CN106706382B true CN106706382B (en) | 2023-07-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611078512.5A Active CN106706382B (en) | 2016-11-29 | 2016-11-29 | Soil springtail collecting device and application thereof |
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| CN (1) | CN106706382B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568169B (en) * | 2017-09-11 | 2020-09-18 | 陈健 | Batched springtail sample collecting device in feeding box |
| CN114594236A (en) * | 2022-03-15 | 2022-06-07 | 西北农林科技大学 | Simple and rapid investigation method for soil springtail of edible fungi |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2763728B1 (en) * | 1997-05-22 | 1999-07-16 | Jeulin Sa | BERLESE APPARATUS FOR HARVESTING AND STUDYING SOIL MICROFAUNA |
| CN102407172A (en) * | 2011-09-07 | 2012-04-11 | 中国科学院南京土壤研究所 | Portable soil animal collection device |
| CN104568563B (en) * | 2015-01-15 | 2018-03-13 | 北京林业大学 | A kind of device and method of soil nematodes separation and Extraction |
| CN204346800U (en) * | 2015-01-15 | 2015-05-20 | 北京林业大学 | A kind of soil nematodes separating extraction device |
| CN206192744U (en) * | 2016-11-29 | 2017-05-24 | 中国科学院南京土壤研究所 | Collect device of springtail sample in follow soil |
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