CN106701599A - Screening method and identification method for mycorrhizal fungus for promoting selenium enriching of vaccinium uliginosum and application thereof - Google Patents
Screening method and identification method for mycorrhizal fungus for promoting selenium enriching of vaccinium uliginosum and application thereof Download PDFInfo
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Abstract
The invention discloses a screening method and identification method for a mycorrhizal fungus for promoting selenium enriching of vaccinium uliginosum and an application thereof. The strain is isolated from vaccinium uliginosum plant roots, a candidate strain is screened through analysis of the vaccinium uliginosum fruit organic selenium content, fruit nutritive element content, mycorrhizal colonization and other test data, and then the fungus is identified to belong to mucoraceae strains by using morphology and molecular biology methods. The strain solves the defect that vaccinium uliginosum cultivation acidic soil is not conducive to absorption of inorganic selenium, is easy to culture and low in cost, can be used for preparation of a mycorrhizal fungus preparation, is used for commercial production of the vaccinium uliginosum, improves the nutritive element absorption of the vaccinium uliginosum, and improves a vaccinium uliginosum root system microbial environment.
Description
Technical field
The invention belongs to strain idenfication and application field, it is related to a kind of screening side of the mycorrhizal fungi for promoting Blueberry selenium-rich
Method and its authentication method and its application.
Background technology
Blueberry is cowberry (Vaccinium uliginosum) also known as bog bilberry, and wild blueberry is referred to as bilberry (size Xingan
Ridge, Yichun) belong to Ericaceae (Ericaceae) genus vaccinium, North America is originated in, there are about 450 kinds.Blueberry can be divided into short clump, half
Gao Cong, clump high and the major class of Vaccinium ashei four.Being rich in Blueberry has various tools such as the multitudinous fruit glucoside of abundant bear, anthocyanidin, flavones
There is the Polyphenols physiologically active ingredient of antioxygenic property, be described as " king of berry ".The cultivating soil of blueberry is acid soil (PH
4.0~5.0), root carefully lacks root hair, and contact area is small, and distribution is shallow, and absorbability is weaker.Under natural conditions, mycorrhizal fungi can be with
Plant forms the relation of mutualism, thus filters out beneficial mycorrhizal fungi and can promote suction of the Vaccinium ashei to nutrient
Receive, contribute to blueberry to grow and improve fruits nutrition and be worth, the growth of improvement blueberry rhizosphere microbial environment and enhancing blueberry
Gesture and the resistivity to pest and disease damage.
Selenium is one of the essential trace elements of the human body, and its physiological function is mainly shown as that selenoprotein and the antioxygen of selenium enzyme are turned into
With.China is a selenium deficiency big country, and 72% area for accounting for Chinese area has a low selenium area, wherein 30% is serious
The natural food Se contents such as selenium deficiency area, grain are relatively low.The big and medium-sized cities such as North China, northeast, northwest belong to selenium deficiency area.China
Various diseases, such as Keshan disease, Kaschin-Beck disease, cardiovascular disease, hepatopathy and tumour have successfully been prevented and treated by crowd's selenium-supply.Using life
The production of thing path for transformation has the toxicity that inorganic selenium can be reduced compared with the Organic Selenium of high bioactivity.And Blueberry is inherently high
The berry of nutritive value, will be very wide as the carrier prospect of selenium bio-organic.
Isolated one plant of mycorrhizal fungi from blueberry plant in 1978 such as Read, tries by its infectivity and tieback
Test, as a result show that growth of the mycorrhizal fungi to blueberry has obvious facilitation.The research in 2007 such as Kosola shows to be inoculated with bacterium
After mycorrhiza fungi, blueberry can strengthen the absorbability of nitrate nitrogen.Liu Yan isolated two plants of mycorhiza from linberry in 2010
Fungi, is mixed and is inoculated on blueberry seedling, is measured after 80 days, finds the seedling gross dry weight and leaf of Arbuscular Mycorrhizal Fungi fungi
Piece number increases by 76.1% and 60.7% than not being inoculated with control respectively；Blueberry seedling aerial part and low part are right be inoculated with simultaneously after
The absorption of nitrogen phosphorus increased, and inoculation treatment is obviously improved the upgrowth situation of blueberry seedling.Yuan Jixin etc. 2013 is isolated
15 inoculations show there is facilitation to blueberry growth of seedling on blue rich seedling, increased to n-p-k element
Absorb.
The Se content of rich selenium fruit is that nutritive value is very high in itself for 10-50 μ g/kg. Blueberries in the market, but because
Root system is weak, acid soil and have impact on the absorption to selenium.Therefore, the conversion for promoting blueberry root inorganic selenium using mycorrhizal fungi is inhaled
Receive, be the effective way of blueberry selenium-rich.
In view of analyze above, the present invention has isolated the mycorrhizal fungi that promotees blueberry selenium-rich from blueberry rhizosphere and has carried out point
Son identification, meanwhile, what the mycorrhizal fungi to isolating and purifying had carried out blueberry seedling stage and plant strain growth phase infects experiment.
The content of the invention
Be conducive to the mycorrhizal fungi of acid soil blueberry selenium-rich the invention aims to obtain, to cultivate Gao Ying
The selenium-rich blueberry of value is supported, for the commodity production of blueberry, there is provided a kind of screening side of the mycorrhizal fungi of rush Blueberry selenium-rich
Method and its authentication method and its application.
To achieve these goals, the present invention is adopted the following technical scheme that：
A kind of screening technique of the mycorrhizal fungi for promoting blueberry selenium-rich, its feature is comprised the following steps：
(1) the blueberry mycorhiza that will be collected carries out disinfection after treatment, is placed in PDA solid mediums of the pH6-8 containing antibiotic
On, cultivated in 25~30 DEG C of constant incubators of temperature, isolated mycorrhizal fungi is inoculated into the PDA trainings containing antibiotic
Support and carry out purifying culture on base, inoculation after purification is in PDA inclined-planes, and 3-4 DEG C of preservation is standby；
(2) by the mycorrhizal fungi tieback obtained by step (1) to blueberry seedling and plant, by quantizing resonance and ICP-MS
The blade of blueberry and the content of fruit nutrient elemeuts are detected, selection promotes the mycorrhizal fungi of blueberry selenium-rich, you can.
The authentication method of the mycorrhizal fungi of described rush blueberry selenium-rich, it is characterised in that
Mycorrhizal fungi after screening is identified by molecular biology and morphological method, mycorrhizal fungi kind is determined
Class.
The authentication method of the mycorrhizal fungi of described rush blueberry selenium-rich, it is characterised in that
Molecular biological variety identification method is：Mycorrhizal fungi after screening is carried out into genome extracting, with the ITS of fungi rDNA
Sequence as universal primer enter performing PCR amplification will be sequenced obtain sequence carried out in GenBank databases BLAST compare point
Analysis, the sequence to homology in Search Results more than 97% carries out sequences match analysis with CLUSTAL-W softwares, and result is transported
With the Neighbor-joining method phylogenetic tree constructions in MEGA6 softwares, the Phylogenetic of mycorrhizal fungi is determined, i.e.,
Can.
The authentication method of the mycorrhizal fungi of described rush blueberry selenium-rich, it is characterised in that
It is determined that the mycorrhizal fungi for being conducive to blueberry selenium-rich is Mucoraceae mucor volume branch Mucor kind M ucor
Circinelloides (numberings：CANV).
The application of the mycorrhizal fungi of described rush blueberry selenium-rich, it is characterised in that
It can be used to prepare mycorrhizal fungi preparation, be applied in the production of blueberry.
The Se contents of the bacterial strain blueberry fruits/plant are inoculated with up to 0.09-0.16 μ g/g, other nutrients Ca, Fe, Zn,
The nutritive element contents such as Mg, Mn have a certain proportion of raising；It is inoculated with blueberry seedling percent, height of seedling, blade N, P, K of the bacterial strain
Content is also accordingly improved.
The separation method of the described mycorrhizal fungi, it is characterised in that step (1) is comprised the following steps：By what is collected
Blueberry mycorrhizal fungi is cleaned after being cut into 3~4cm root segments with distilled water, then is placed on specification to use 75% wine in the culture dish of 90mm
Essence, 75% alcohol and 0.1% mercuric chloride are combined and carry out surface sterilizing, and the root sample after sterilizing is cut into 0.5cm and puts with sterilized scissors
Cultivated in the PDA culture medium containing streptomysin, culture dish specification is 90mm, trained in 25~30 DEG C of constant incubators of temperature
Support, cultivate 3~5d, being put into transfer needle picking individual colonies carries out purifying culture in the PDA culture medium containing streptomysin, after purification
Inoculation is in PDA inclined-planes, 4 DEG C of preservations.
Described nutritive element content is determined, it is characterised in that comprised the following steps：Step (2) is used, inductive etc.
Gas ions mass spectrography (ICP-MS) measures the total selenium of fruit and nutritive element content；Using quantizing resonance method detection blueberry blade Se,
N, P, K relative amount.
Described blueberry selenium and the assay method of nutritive element content are：
0.3g selenium-rich Blueberry dry powder is taken, plus 9~after 11ml digestive juices shake up, described digestive juice is concentrated nitric acid：It is high
Hypochlorous acid=4:1 configuration is obtained, and is stood overnight, and is placed on electric hot plate and is heated to that organic matter is decomposed or color and luster is deepened, add 1~
3ml digestive juices continue to digest, and control temperature is less than 200 DEG C, is so clarified to digestive juice repeatedly, there is HClO4White cigarette is emerged, and is continued
1~3ml of solution is heated to, with 60% dense HCl constant volumes in 25ml volumetric flasks, then adds 0.5%NaBH4To reaction end, use
ICP-MS, inductively coupled plasma mass spectrometry measurement selenium and nutritive element content.
Described blueberry selenium relative amount detection method is：
0.2g blueberry plant leafs are placed on resonating disk, by the frequency in micro- magnetic field of grip sensor determination sample and
Energy, through instrument amplification, the standard " quantizing resonance spectrum " of the nutritive index after computer disposal and inside equipment and instrument compares, defeated
Go out corresponding by bearing positive relative quantity value.
The Morphological Identification method of mycorrhizal fungi, comprises the following steps：Sieved according to quantizing resonance and ICP-MS testing results
Rush blueberry selenium-rich dominant strain is selected, the Preliminary Identification of bacterial strain is carried out by bacterium colony mode of appearance, mycelia optical microscope,
And the experiment of infecting using Trypan Blue blueberry root carries out the poor morphology opposite sex and compares.See Fig. 2.According to Fungal identification handbook,
Carry out morphological classification.
The molecular biological variety identification method of mycorrhizal fungi, comprises the following steps：Mycorrhizal fungi after screening is carried out into gene
Group extracting, entering performing PCR amplification as universal primer using the ITS sequence of fungi rDNA will be sequenced the sequence for obtaining in GenBank numbers
Analysis is compared according to BLAST is carried out in storehouse, the sequence to homology in Search Results more than 97% is carried out with CLUSTAL-W softwares
Sequences match is analyzed, and to the Neighbor-joining method phylogenetic tree constructions in application of results MEGA6 softwares, determines mycorhiza
The Phylogenetic of fungi, completes Molecular Identification.
Accompanying drawing 1 is shown in the separation and authentication method technical route for promoting blueberry selenium-rich mycorrhizal fungi.
By above method, we have filtered out a kind of bacterium for promoting blueberry acid soil selenium-rich from blueberry rhizosphere microorganism
Mycorrhiza fungi, mucor volume branch Mucor kind Mucor is accredited as using morphological observation and molecular biology method
circinelloides.See accompanying drawing 2, Fig. 3.
One plant of described application of the mycorrhizal fungi of rush blueberry selenium-rich, can be used to prepare mycorrhizal fungi preparation, be inoculated in indigo plant
Certain kind of berries seedling, promotes it to grow；Blueberry plant is inoculated in, the content of its fruit selenium is improved, other nutrients Ca, Fe, Zn, M n,
The nutritive element contents such as Cr also have a certain proportion of raising.
The potted plant cultural method of selenium-rich blueberry seedling, comprises the following steps：Compost is put into 121 DEG C of high pressure steam sterilizations
20min, is divided into the aliquot of 80g equivalent, pours into aseptic 10cm × 8cm × 7cm Culture basins, with oxalic acid adjust P in soil H be 4.0~
5.5, a hole of the 3cm × 3cm of deep 5cm is dug, 2cm × 2cm bacteria cakes 2 are added, blueberry seedling is moved into.Blueberry seedling is placed in
Ventilation constant temperature (25 DEG C) photoenvironment culture, pours sterilized water.After blueberry transplantation of seedlings is survived, root applies the sub- selenium that concentration is 40mg/ml
Acid sodium solution to soil Se content reaches 0.04mg/kg, and the mycorrhizal fungi of inoculated and cultured is applied selenium fertilizer once, three are applied altogether at interval of 15d
It is secondary.
Selenium-rich blueberry plant cultur method, comprises the following steps：By by the plant ash and soil after 121 DEG C of autoclavings
According to 1：3 ratios, are well mixed, and it is 4.0~5.5 to adjust P in soil H with oxalic acid, adds selenium fertilizer soil Se content is reached 0.04mg/
After kg, blueberry plant is transplanted.The mycorrhizal fungi of inoculated and cultured, it is 0.04mg/kg that interval 15d adds selenium fertilizer to soil Se content.
Periodically watered (oxalic acid adjust PH be 4.5~5.5) management.
Beneficial effects of the present invention：
(1) Screening and Identification of the present invention obtains the bacterial strain of mucor volume branch Mucor kind Mucor circinelloides can promote
Enter the selenium-rich of Blueberry, improve Blueberry nutritive value, reduce blueberry growing and cultivation management cost.
(2) the selenium-rich blueberry for connecing mycorrhizal fungi is significantly improved than not connecing bacterium blueberry Se content, and the content of selenium-rich blueberry selenium can
Up to 0.09-0.163 μ g/g
(3) other nutritive element contents of blueberry after bacterium are connect also to significantly improve：Ca contents are up to 200-500 μ g/g, Zn contents
Up to 1-5 μ g/g, Fe content up to 5-30 μ g/g, K content up to 7-15mg/g, the content of Mn up to 1.5-3.0 μ g/g,
The content of Cr is up to 0.5-1.5 μ g/g.
(4) blueberry transplanting shoot survival percent improves 20-40%, and height of seedling improves 5-10%.
Brief description of the drawings
Fig. 1 is the technical route schematic diagram that mycorrhizal fungi promotees blueberry selenium-rich；
Fig. 2 is the mucor volume branch Mucor kind Mucor circinelloides fungi pictures for promoting blueberry selenium-rich；
Fig. 3 is mycorrhizal fungi systematic growth tree graph.
Specific embodiment
Embodiment 1：The culture of Vaccinium ashei seedling and the inoculation of mycorrhizal fungi：Compost is put into 121 DEG C of high steams
Sterilizing 20min, is divided into the aliquot of 80g equivalent, pours into aseptic 10cm × 8cm × 7cm Culture basins, is with oxalic acid tune P in soil H
4.0~5.5, a hole of the 3cm × 3cm of deep 5cm is dug, 2cm × 2cm bacteria cakes 2 are added, blueberry seedling is moved into.By blueberry children
Seedling is placed in ventilation constant temperature (25 DEG C) photoenvironment culture, pours sterilized water.After blueberry transplantation of seedlings is survived, root applies concentration for 40mg/ml
Sodium selenite solution to soil Se content reach 0.04mg/kg, the mycorrhizal fungi CANV of inoculated and cultured, then at interval of 15d
Apply selenium fertilizer once, common Shi Sanci.After third time selenium fertilizer has applied 15d, plucking selenium-rich blueberry seedling leaves carries out Quantum resonance detection.
Survival rate after seedling replanting improves 38.9%, height of seedling improves 9.1%, Quantum resonance detection, selenium, nitrogen, phosphorus, potassium
Content is also accordingly improved.It is shown in Table 1.
Selenium-rich blueberry seedling leaves N, P, the detection of K relative amounts of the Arbuscular Mycorrhizal Fungi fungi of table 1
Embodiment 2：The selenium-rich culturing of Vaccinium ashei plant：To be pressed by plant ash and soil after 121 DEG C of autoclavings
According to 1：3 ratios, are well mixed, and it is 4.0~5.5 to adjust P in soil H with oxalic acid, adds selenium fertilizer soil Se content is reached 0.04mg/kg
Afterwards, blueberry plant is transplanted.It is 0.04mg/kg that interval 15d adds selenium fertilizer to soil Se content.Periodically being watered, (oxalic acid adjusts PH
It is 4.5~5.5) management.After blueberry plantlet of transplant is survived, root is applied sodium selenite solution to the Selenium in Soil that concentration is 40mg/ml and is contained
Amount reaches 0.04mg/kg, the mycorrhizal fungi CANV of inoculated and cultured, then applies selenium fertilizer once at interval of 15d, common Shi Sanci.3rd
After secondary selenium fertilizer has applied 15d, the selenium-rich Blueberry of harvesting ripe carries out ICP-MS detections.Testing result is shown in Table 2.
The measurement result of selenium and nutritive element content in the selenium-rich blueberry of the Arbuscular Mycorrhizal Fungi fungi of table 2.
Claims (5)
1. it is a kind of promote blueberry selenium-rich mycorrhizal fungi screening technique, it is characterised in that comprise the following steps：
The blueberry mycorhiza that will be collected carries out disinfection after treatment, is placed on PDA solid mediums of the pH6-8 containing antibiotic, in temperature
Cultivated in 25~30 DEG C of constant incubators of degree, isolated mycorrhizal fungi is inoculated into the PDA culture medium containing antibiotic
Purifying culture is carried out, inoculation after purification is in PDA inclined-planes, and 3-4 DEG C of preservation is standby；
(2) by step（1）The mycorrhizal fungi tieback of gained is examined on blueberry seedling and plant by quantizing resonance and ICP-MS
The blade of blueberry and the content of fruit nutrient elemeuts are surveyed, selection promotes the mycorrhizal fungi of blueberry selenium-rich, you can.
2. it is a kind of it is as claimed in claim 1 promote blueberry selenium-rich mycorrhizal fungi authentication method, it is characterised in that
Mycorrhizal fungi after screening is identified by molecular biology and morphological method, mycorrhizal fungi species is determined.
3. it is according to claim 2 promote blueberry selenium-rich mycorrhizal fungi authentication method, it is characterised in that
Molecular biological variety identification method is：Mycorrhizal fungi after screening is carried out into genome extracting, with the ITS of fungi rDNA
Sequence as universal primer enter performing PCR amplification will be sequenced obtain sequence BLAST ratios are carried out in GenBank databases
To analysis, the sequence to homology in Search Results more than 97% carries out sequences match analysis with CLUSTAL-W softwares,
To the Neighbor-joining method phylogenetic tree constructions in application of results MEGA6 softwares, the system for determining mycorrhizal fungi
Development status, you can.
4. it is according to claim 2 promote blueberry selenium-rich mycorrhizal fungi authentication method, it is characterised in that
It is determined that the mycorrhizal fungi for being conducive to blueberry selenium-rich is Mucoraceae mucor volume branch Mucor kind Mucor circinelloides.
5. a kind of application of the mycorrhizal fungi for promoting blueberry selenium-rich as claimed in claim 1, it is characterised in that
It can be used to prepare mycorrhizal fungi preparation, be applied in the production of blueberry.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114931053A (en) * | 2022-04-29 | 2022-08-23 | 广西南亚热带农业科学研究所 | Cultivation method of macadimia nut excellent germplasm resources |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6146880A (en) * | 1998-02-19 | 2000-11-14 | The United States Of America As Represented By The Secretary Of Agriculture | Methods for lyophilizing and using ericoid mycorrhizal fungi |
| CN102220246A (en) * | 2011-05-18 | 2011-10-19 | 辽宁省农业科学院 | Blueberry mycorrhizal fungi (coprinus micaceus) and separation method and application thereof |
| CN104230529A (en) * | 2014-08-22 | 2014-12-24 | 合肥四方磷复肥有限责任公司 | Special higher-utilization-ratio environment-friendly coated fertilizer for blueberries and preparation method thereof |
-
2017
- 2017-01-17 CN CN201710035222.0A patent/CN106701599A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6146880A (en) * | 1998-02-19 | 2000-11-14 | The United States Of America As Represented By The Secretary Of Agriculture | Methods for lyophilizing and using ericoid mycorrhizal fungi |
| CN102220246A (en) * | 2011-05-18 | 2011-10-19 | 辽宁省农业科学院 | Blueberry mycorrhizal fungi (coprinus micaceus) and separation method and application thereof |
| CN104230529A (en) * | 2014-08-22 | 2014-12-24 | 合肥四方磷复肥有限责任公司 | Special higher-utilization-ratio environment-friendly coated fertilizer for blueberries and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘丽丽等: "丛枝菌根真菌（AMF）对红阳猕猴桃叶片富硒能力及光合特性的影响", 《食品工业科技》 * |
| 肖军等: "蓝莓菌根菌的分离与回接试验", 《辽宁农业科学》 * |
| 赵磊等: "促兔眼蓝莓生长菌根真菌的分离与鉴定", 《生物学杂志》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114931053A (en) * | 2022-04-29 | 2022-08-23 | 广西南亚热带农业科学研究所 | Cultivation method of macadimia nut excellent germplasm resources |
| CN114931053B (en) * | 2022-04-29 | 2023-06-27 | 广西南亚热带农业科学研究所 | Cultivation method of excellent heterogeneous material resources of macadimia nut |
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Application publication date: 20170524 |