CN106675786B - Method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis and taxus chinensis essential oil mixture - Google Patents
Method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis and taxus chinensis essential oil mixture Download PDFInfo
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- CN106675786B CN106675786B CN201610716842.6A CN201610716842A CN106675786B CN 106675786 B CN106675786 B CN 106675786B CN 201610716842 A CN201610716842 A CN 201610716842A CN 106675786 B CN106675786 B CN 106675786B
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/0003—Compounds of unspecified constitution defined by the chemical reaction for their preparation
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
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- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a method for extracting an essential oil mixture from branches, leaves, bark and roots of taxus chinensis and the taxus chinensis essential oil mixture, wherein the extraction method comprises the following steps: selecting, cleaning, drying or naturally airing, crushing, carrying out enzymolysis, drying, grinding, adding salt and extracting the taxus chinensis branches, leaves, skins and roots. The invention provides a method for extracting a taxus chinensis essential oil mixture which is free of toxin and contains macro elements and trace elements from taxus chinensis branches, leaves, barks and roots. The taxus chinensis essential oil mixture is used for preparing daily necessities of taxus chinensis or external drugs, and has obvious curative effect in the process of treating pains as an active ingredient.
Description
Technical Field
The invention belongs to the field of extraction of plant effective components, and particularly relates to a method for extracting an essential oil mixture from branches, leaves, bark and roots of taxus chinensis and the taxus chinensis essential oil mixture.
Background
Taxus chinensis belongs to shallow root plants, has unobvious main roots and developed lateral roots, and is a natural rare anti-cancer plant which is generally recognized to be endangered to be extinct in the world. The branches, leaves, bark and roots of the taxus chinensis contain organic substances such as paclitaxel, taxus flavone and taxus chinensis polysaccharide, also contain macro elements such as phosphorus, calcium, magnesium, sodium and potassium, and also contain trace elements such as iron, copper, zinc, cobalt, molybdenum, manganese, vanadium, tin, selenium, iodine, nickel, strontium and the like.
The existing method for extracting the taxus chinensis essential oil mixture has several defects, the first method is that toxins in the taxus chinensis are not completely removed, and the second method is that macro elements and trace elements in the taxus chinensis are not extracted and are discarded, so that the waste of resources is caused.
Disclosure of Invention
The invention provides a method for extracting a taxus chinensis essential oil mixture which is free of toxin and contains macro elements and trace elements from taxus chinensis branches, leaves, barks and roots.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis comprises the following steps:
1) selecting branches, leaves, bark and roots of the taxus chinensis: 35 to 45 parts by weight of yew branches with the diameter less than 1 cm, 25 to 35 parts by weight of yew leaves, 5 to 15 parts by weight of yew skins, and 15 to 25 parts by weight of yew roots;
the selection of the branches, leaves, bark and roots of the taxus chinensis has a certain reason, and different from other inventions, the invention selects four parts of the branches, leaves, bark and roots of the taxus chinensis. The matching of the four parts can play a synergistic effect: not only can maximize the effective ingredients of the Chinese yew; moreover, the content of toxin can be reduced to the maximum extent through the interaction of different substances of branches, leaves, barks and roots.
The branch with the diameter less than 1 cm is selected from the taxus chinensis branches, because the branch with the diameter less than 1 cm has low toxicity, the toxin in the enzymolysis stage can be conveniently eliminated; and the branches of the yew with smaller diameter contain more effective medicinal components.
2) Cleaning: cleaning the branches, leaves, skins and roots of the taxus chinensis in the step 1) with clear water;
3) drying or naturally airing: drying or airing the cleaned branches, leaves, skins and roots of the taxus chinensis;
4) crushing: crushing the branches, leaves, bark and roots of the taxus chinensis in the step 3);
pulverizing branches, leaves, skins and roots of the taxus chinensis by a pulverizer or other crushing machines, and facilitating enzymolysis detoxification or extraction in subsequent steps.
5) Enzymolysis: carrying out enzymolysis on crushed branches, leaves, bark and roots of the taxus chinensis, 0.5 to 1 weight part of cellulase and 5 to 10 weight parts of clear water at the temperature of 20 ℃ to 30 ℃ for 8 hours to 15 hours;
cellulose in branches, leaves, skins and roots of the taxus chinensis can be degraded into glucose by using cellulase on one hand, and nutrient components in the taxus chinensis essential oil mixture are increased; on the other hand, the cellulose of the branches, leaves, bark and roots of the taxus chinensis can be degraded, so that the toxin in the branches, leaves, bark and roots of the taxus chinensis can be conveniently removed.
6) Drying: fishing out the branches, leaves, skins and roots of the taxus chinensis subjected to enzymolysis in the step 5), and drying at the temperature of 60-80 ℃;
7) grinding: grinding the dried branches, leaves, barks and roots of the taxus chinensis in the step 6) into powder with the granularity of 100 meshes to 200 meshes;
8) adding salt: mixing the taxus branch, leaf, bark and root powder with 5-10 parts by weight of salt to obtain a taxus branch, leaf, bark and root powder mixture, wherein the salt is one or more of edible potassium carbonate, edible potassium chloride, edible sodium carbonate or edible sodium chloride;
9) extraction by a leaching method: extracting in two steps, adding purified water into the powder mixture obtained in the step 8) to extract macro and trace elements and oily components, and extracting paclitaxel and organic substances with 100-300 parts by weight of edible alcohol or Chinese liquor.
The purpose of adding salt is to 'pickle' the powder of the branches, leaves, barks and roots of the taxus chinensis, and in the extraction stage, the cells in the branches, leaves, barks and roots of the taxus chinensis are in the extraction liquid with high salinity, so that the cell walls are dehydrated and broken, and the nutrient substances in the cell walls are easily released.
The extraction method is different from the common extraction method, and by means of the leaching method, the taxus chinensis branch, leaf, bark and root powder is in full and circular contact with edible alcohol in a reaction kettle, so that the extraction effect can be greatly improved.
The addition of edible alcohol or Chinese liquor can dissolve organic substances such as paclitaxel, taxoflavone and taxus chinensis polysaccharide in branches, leaves, barks and roots of yew, and can also dissolve macro elements (phosphorus, calcium, magnesium, sodium, potassium and the like) and trace elements (iron, copper, zinc, cobalt, molybdenum, manganese, vanadium, tin, selenium, iodine, nickel, strontium and the like), thereby improving the extraction effect.
Preferably, the method for extracting the essential oil mixture from the branches, leaves, bark and roots of the taxus chinensis is selected from taxus mairei.
The taxus mairei has the unique advantages, contains abundant nutrient substances, has low heavy metal content and meets the national standard.
Preferably, in the method for extracting essential oil mixture from taxus branch, leaf, bark and root, the temperature of the grinding process in the step 7) is kept between 20 ℃ and 40 ℃.
The grinding process, if the temperature is too high, can inactivate the nutrients in the branches, leaves, bark and roots of the yew, and can reduce the pharmaceutical effect. Because the branches, leaves, barks and roots of the taxus chinensis generate friction in the grinding process, the internal temperature of the grinding vessel is higher, the invention can arrange circulating water outside the grinding vessel to cool the grinding vessel, so that the temperature of the grinding vessel is not too high.
Preferably, the method for extracting essential oil mixture from taxus branch, leaf, bark and root is prepared by the method for extracting essential oil mixture from taxus branch, leaf, bark and root as claimed in any one of claims 1 to 3.
The essential oil mixture contains organic substances such as paclitaxel, yew flavone and yew polysaccharide, macro elements such as phosphorus, calcium, magnesium, sodium and potassium, and trace elements such as iron, copper, zinc, cobalt, molybdenum, manganese, vanadium, tin, selenium, iodine, nickel and strontium.
Paclitaxel is mainly suitable for ovarian cancer and breast cancer, and has therapeutic effect on lung cancer, carcinoma of large intestine, melanoma, head and neck cancer, lymphoma, and cerebroma.
Taxus chinensis flavone has effects of regulating cardiovascular system, resisting inflammation and immunity, resisting microorganism, treating peptic ulcer, and resisting tumor.
The polysaccharide of Taxus chinensis has excellent effects of improving immunity, inhibiting tumor growth and tumor metastasis, and enhancing tolerance.
Detecting the extracted taxus chinensis essential oil mixture, and respectively measuring the heavy metal content (shown in table 1), the macroelement content (shown in table 2) and the trace element content (shown in table 3):
TABLE 1 content of heavy metals in Taxus chinensis essential oil mixture
TABLE 2 Macro element content in Taxus chinensis essential oil mixture
| Element(s) | Content (μ g/g) |
| Phosphorus (P) | 1850.49 |
| Calcium carbonate | 9363.17 |
| Magnesium alloy | 848.52 |
| Sodium salt | 17.38 |
| Potassium salt | 5871.53 |
TABLE 3 content of trace elements in Taxus chinensis essential oil mixture
| Element(s) | Content (μ g/g) | Element(s) | Content (μ g/g) |
| Iron | 585.63 | Vanadium oxide | 1.04 |
| Copper (Cu) | 4.42 | Tin (Sn) | 0.11 |
| Zinc | 96.55 | Selenium | 0.24 |
| Cobalt | 0.31 | Iodine | 0.40 |
| Molybdenum (Mo) | 0.20 | Nickel (II) | 1.72 |
| Manganese oxide | 419.45 | Strontium salt | 43.20 |
As can be seen from the above table, the essential oil mixture of yew contains abundant macro elements of phosphorus, calcium, magnesium, sodium and potassium, and also contains trace elements such as iron, copper, zinc, cobalt, molybdenum, manganese, vanadium, tin, selenium, iodine, nickel, strontium, etc. The macroelements and microelements in the Chinese yew essential oil mixture and organic substances such as paclitaxel, yew flavone and Chinese yew polysaccharide form medicinal components of the Chinese yew essential oil mixture. The taxus chinensis essential oil mixture disclosed by the invention contains organic substances, macro and trace chemical elements, and the active ingredient content of the taxus chinensis daily necessities or external drugs prepared from the taxus chinensis essential oil mixture is high, and the medicinal effect of the substances is obvious due to the synergistic effect of the macro and trace chemical elements and the organic substances. The obtained essential oil mixture of Taxus chinensis can be used for preparing daily necessities or topical medicine of Taxus chinensis as active ingredient, for example, the essential oil mixture of Taxus chinensis can be added into soap, and medicine for treating dermatosis, rheumatism, scald, promoting blood circulation, caring skin, oral diseases, gingival hemorrhage, constipation, and hemostasis. Experiments show that the taxus chinensis essential oil mixture has obvious curative effect in the process of treating the pain.
Detailed Description
The following detailed description is given with reference to the embodiments in order to explain technical contents, structural features, and achieved objects and effects of the present invention in detail.
Example 1
The specific process method of the embodiment is as follows:
a method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis comprises the following steps:
1) selecting branches, leaves, bark and roots of the taxus chinensis: 35g of yew branches with the diameter less than 1 cm, 35g of yew leaves, 5g of yew skins and 25g of yew roots;
2) cleaning: cleaning the branches, leaves, skins and roots of the taxus chinensis in the step 1) with clear water;
3) drying or naturally airing: drying or airing the cleaned branches, leaves, skins and roots of the taxus chinensis;
4) crushing: crushing the branches, leaves, bark and roots of the taxus chinensis in the step 3);
5) enzymolysis: carrying out enzymolysis on the crushed branches, leaves, bark and roots of the taxus chinensis, 0.5g of cellulase and 5g of clear water at the temperature of 30 ℃ for 8 hours;
6) drying: fishing out branches, leaves, skins and roots of the taxus chinensis subjected to enzymolysis in the step 5), and drying at the temperature of 60 ℃;
7) grinding: grinding the dried branches, leaves, skins and roots of the taxus chinensis in the step 6) into powder with the granularity of 200 meshes; the temperature in the grinding process is kept at 20 ℃;
8) adding salt: mixing the powder of the branches, leaves, bark and roots of the taxus chinensis with 5g of edible potassium carbonate to obtain a powder mixture of the branches, leaves, bark and roots of the taxus chinensis;
9) extraction by a leaching method: extracting in two steps, namely adding purified water into the powder mixture in the step 8) to extract macro and trace elements and oily components, and extracting the powder mixture in the step 8) by using 300g of edible alcohol.
The taxus chinensis essential oil mixture is prepared by the method for extracting the essential oil mixture from the branches, leaves, bark and roots of the taxus chinensis.
Example 2
The specific process method of the embodiment is as follows:
a method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis comprises the following steps:
1) selecting branches, leaves, bark and roots of the taxus chinensis: 45g of yew branches with the diameter less than 1 cm, 25g of yew leaves, 15g of yew skins and 15g of yew roots;
2) cleaning: cleaning the branches, leaves, skins and roots of the taxus chinensis in the step 1) with clear water;
3) drying or naturally airing: drying or airing the cleaned branches, leaves, skins and roots of the taxus chinensis;
4) crushing: crushing the branches, leaves, bark and roots of the taxus chinensis in the step 3);
5) enzymolysis: carrying out enzymolysis on the crushed branches, leaves, bark and roots of the taxus chinensis, 1g of cellulase and 10g of clear water at the temperature of 20 ℃ for 15 hours;
6) drying: fishing out branches, leaves, skins and roots of the taxus chinensis subjected to enzymolysis in the step 5), and drying at the temperature of 80 ℃;
7) grinding: grinding the dried branches, leaves, skins and roots of the taxus chinensis in the step 6) into powder with the granularity of 100 meshes; the temperature in the grinding process is kept at 40 ℃;
8) adding salt: mixing the powder of the branches, leaves, bark and roots of the taxus chinensis with 6g of edible sodium carbonate to obtain a powder mixture of the branches, leaves, bark and roots of the taxus chinensis;
9) extraction by a leaching method: extracting in two steps, namely adding purified water into the powder mixture in the step 8) to extract macro and trace elements and oily components, and extracting the powder mixture in the step 8) by using 100g of white spirit.
The taxus chinensis essential oil mixture is prepared by the method for extracting the essential oil mixture from the branches, leaves, bark and roots of the taxus chinensis.
Example 3
The specific process method of the embodiment is as follows:
a method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis comprises the following steps:
1) selecting branches, leaves, bark and roots of the taxus chinensis: 40g of yew branches with the diameter less than 1 cm, 30g of yew leaves, 10g of yew skins and 20g of yew roots;
2) cleaning: cleaning the branches, leaves, skins and roots of the taxus chinensis in the step 1) with clear water;
3) drying or naturally airing: drying or airing the cleaned branches, leaves, skins and roots of the taxus chinensis;
4) crushing: crushing the branches, leaves, bark and roots of the taxus chinensis in the step 3);
5) enzymolysis: carrying out enzymolysis on the crushed branches, leaves, bark and roots of the taxus chinensis, 0.6g of cellulase and 6g of clear water at the temperature of 22 ℃ for 9 hours;
6) drying: fishing out branches, leaves, skins and roots of the taxus chinensis subjected to enzymolysis in the step 5), and drying at the temperature of 65 ℃;
7) grinding: grinding the dried branches, leaves, skins and roots of the taxus chinensis in the step 6) into powder with the granularity of 180 meshes; the temperature in the grinding process is kept at 25 ℃;
8) adding salt: mixing the powder of the branches, leaves, bark and roots of the taxus chinensis with 3g of edible potassium carbonate and 3g of edible potassium chloride to obtain a powder mixture of the branches, leaves, bark and roots of the taxus chinensis;
9) extraction by a leaching method: extracting in two steps, namely adding purified water into the powder mixture in the step 8) to extract macro and trace elements and oily components, and extracting the powder mixture in the step 8) by using 200g of edible alcohol.
The taxus chinensis essential oil mixture is prepared by the method for extracting the essential oil mixture from the branches, leaves, bark and roots of the taxus chinensis.
Example 4
The specific process method of the embodiment is as follows:
a method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis comprises the following steps:
1) selecting branches, leaves, bark and roots of the taxus chinensis: 35g of yew branches with the diameter less than 1 cm, 25g of yew leaves, 5g of yew skins and 15g of yew roots;
2) cleaning: cleaning the branches, leaves, skins and roots of the taxus chinensis in the step 1) with clear water;
3) drying or naturally airing: drying or airing the cleaned branches, leaves, skins and roots of the taxus chinensis;
4) crushing: crushing the branches, leaves, bark and roots of the taxus chinensis in the step 3);
5) enzymolysis: carrying out enzymolysis on the crushed branches, leaves, bark and roots of the taxus chinensis with 0.7g of cellulase and 7g of clear water at the temperature of 25 ℃ for 13 hours;
6) drying: fishing out branches, leaves, skins and roots of the taxus chinensis subjected to enzymolysis in the step 5), and drying at the temperature of 75 ℃;
7) grinding: grinding the dried branches, leaves, skins and roots of the taxus chinensis in the step 6) into powder with the granularity of 130 meshes; the temperature in the grinding process is kept at 35 ℃;
8) adding salt: mixing the powder of the branches, leaves, bark and roots of the taxus chinensis with 4g of edible sodium carbonate and 4g of edible sodium chloride to obtain a powder mixture of the branches, leaves, bark and roots of the taxus chinensis;
9) extraction by a leaching method: extracting in two steps, namely adding purified water into the powder mixture in the step 8) to extract macro and trace elements and oily components, and extracting the powder mixture in the step 8) by using 150g of white spirit.
The taxus chinensis essential oil mixture is prepared by the method for extracting the essential oil mixture from the branches, leaves, bark and roots of the taxus chinensis.
Example 5
The specific process method of the embodiment is as follows:
a method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis comprises the following steps:
1) selecting branches, leaves, bark and roots of the taxus chinensis: 40g of yew branches with the diameter less than 1 cm, 30g of yew leaves, 10g of yew skins and 20g of yew roots;
2) cleaning: cleaning the branches, leaves, skins and roots of the taxus chinensis in the step 1) with clear water;
3) drying or naturally airing: drying or airing the cleaned branches, leaves, skins and roots of the taxus chinensis;
4) crushing: crushing the branches, leaves, bark and roots of the taxus chinensis in the step 3);
5) enzymolysis: carrying out enzymolysis on the crushed branches, leaves, bark and roots of the taxus chinensis with 0.8g of cellulase and 9g of clear water at the temperature of 27 ℃ for 11 hours;
6) drying: fishing out branches, leaves, skins and roots of the taxus chinensis subjected to enzymolysis in the step 5), and drying at the temperature of 70 ℃;
7) grinding: grinding the dried branches, leaves, skins and roots of the taxus chinensis in the step 6) into powder with the granularity of 150 meshes; the temperature in the grinding process is kept at 30 ℃;
8) adding salt: mixing the powder of the branches, leaves, bark and roots of the taxus chinensis with 2g of edible potassium carbonate, 3g of edible potassium chloride, 2g of edible sodium carbonate and 3g of edible sodium chloride to obtain a powder mixture of the branches, leaves, bark and roots of the taxus chinensis;
9) extraction by a leaching method: extracting in two steps, namely adding purified water into the powder mixture in the step 8) to extract macro and trace elements and oily components, and extracting the powder mixture in the step 8) by using 250g of edible alcohol.
The taxus chinensis essential oil mixture is prepared by the method for extracting the essential oil mixture from the branches, leaves, bark and roots of the taxus chinensis.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (2)
1. A method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis is characterized by comprising the following steps:
1) selecting branches, leaves, bark and roots of the taxus chinensis: 35 to 45 parts by weight of yew branches with a diameter of less than 1 cm, 25 to 35 parts by weight of yew leaves, 5 to 15 parts by weight of yew skins, 15 to 25 parts by weight of yew roots, wherein the yew is selected from taxus chinensis var mairei;
2) cleaning: cleaning the branches, leaves, skins and roots of the taxus chinensis in the step 1) with clear water;
3) drying or naturally airing: drying or airing the cleaned branches, leaves, skins and roots of the taxus chinensis;
4) crushing: crushing the branches, leaves, bark and roots of the taxus chinensis in the step 3);
5) enzymolysis: carrying out enzymolysis on crushed branches, leaves, bark and roots of the taxus chinensis, 0.5 to 1 weight part of cellulase and 5 to 10 weight parts of clear water at the temperature of 20 ℃ to 30 ℃ for 8 hours to 15 hours;
6) drying: fishing out the branches, leaves, skins and roots of the taxus chinensis subjected to enzymolysis in the step 5), and drying at the temperature of 60-80 ℃;
7) grinding: grinding the dried branches, leaves, barks and roots of the taxus chinensis in the step 6) into powder with the granularity of 100-200 meshes, wherein the temperature in the grinding process is kept at 20-40 ℃;
8) adding salt: mixing the taxus branch, leaf, bark and root powder with 5-10 parts by weight of salt to obtain a taxus branch, leaf, bark and root powder mixture, wherein the salt is one or more of edible potassium carbonate, edible potassium chloride, edible sodium carbonate or edible sodium chloride;
9) extraction by a leaching method: extracting in two steps, wherein in the first step, purified water is added into the powder mixture in the step 8) to extract macro-elements, trace elements and oily components, and in the second step, 100 to 300 parts by weight of edible alcohol or white spirit is used for extracting paclitaxel and organic substances in the powder mixture in the step 8).
2. A yew essential oil mixture, characterized in that the yew essential oil mixture is prepared by the method of extracting an essential oil mixture from yew branches, leaves, bark, roots according to claim 1.
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| CN110327158A (en) * | 2019-08-06 | 2019-10-15 | 湖南俏妃卫生用品有限公司 | A kind of bamboo fibre sanitary towel with cloth cover containing functional drug |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634268A (en) * | 2004-09-23 | 2005-07-06 | 李万祥 | Method for preparing yew extractive and cancer resisting Chinese medicine by said extractive |
| CN101245360A (en) * | 2008-03-24 | 2008-08-20 | 江西德宇集团 | Biological enzymatic hydrolysis extraction technique of paclitaxel from south yew |
| CN101838588A (en) * | 2010-05-13 | 2010-09-22 | 合肥工业大学 | Method for extracting essential oil of taxus media |
| CN102160893A (en) * | 2011-02-25 | 2011-08-24 | 中国医学科学院医学生物学研究所 | Taxus polysaccharide immunologic adjuvant and influenza vaccine containing same |
| CN104248649A (en) * | 2014-09-24 | 2014-12-31 | 江苏红豆杉药业有限公司 | Preparation method for taxus chinensis extractive and application of taxus chinensis extractive |
| CN104250308A (en) * | 2014-09-24 | 2014-12-31 | 江苏红豆杉药业有限公司 | Extraction method of active polysaccharides and application of active polysaccharides |
| CN104450316A (en) * | 2014-12-10 | 2015-03-25 | 江西省喜果绿化有限公司 | Taxus chinensis soap for dry skin |
| CN105017182A (en) * | 2015-07-06 | 2015-11-04 | 贵州玉福临科技开发有限公司 | Method for highly efficiently extracting paclitaxel from Chinese yew |
-
2016
- 2016-08-25 CN CN201610716842.6A patent/CN106675786B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1634268A (en) * | 2004-09-23 | 2005-07-06 | 李万祥 | Method for preparing yew extractive and cancer resisting Chinese medicine by said extractive |
| CN101245360A (en) * | 2008-03-24 | 2008-08-20 | 江西德宇集团 | Biological enzymatic hydrolysis extraction technique of paclitaxel from south yew |
| CN101838588A (en) * | 2010-05-13 | 2010-09-22 | 合肥工业大学 | Method for extracting essential oil of taxus media |
| CN102160893A (en) * | 2011-02-25 | 2011-08-24 | 中国医学科学院医学生物学研究所 | Taxus polysaccharide immunologic adjuvant and influenza vaccine containing same |
| CN104248649A (en) * | 2014-09-24 | 2014-12-31 | 江苏红豆杉药业有限公司 | Preparation method for taxus chinensis extractive and application of taxus chinensis extractive |
| CN104250308A (en) * | 2014-09-24 | 2014-12-31 | 江苏红豆杉药业有限公司 | Extraction method of active polysaccharides and application of active polysaccharides |
| CN104450316A (en) * | 2014-12-10 | 2015-03-25 | 江西省喜果绿化有限公司 | Taxus chinensis soap for dry skin |
| CN105017182A (en) * | 2015-07-06 | 2015-11-04 | 贵州玉福临科技开发有限公司 | Method for highly efficiently extracting paclitaxel from Chinese yew |
Non-Patent Citations (1)
| Title |
|---|
| 云南红豆杉中紫杉烷类化合物的提取工艺优化;徐芸，等;《化学与生物工程》;20160229;第33卷(第01期);第39-42页 * |
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