CN101245360A - Biological enzymatic hydrolysis extraction technique of paclitaxel from south yew - Google Patents
Biological enzymatic hydrolysis extraction technique of paclitaxel from south yew Download PDFInfo
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- CN101245360A CN101245360A CNA2008100863797A CN200810086379A CN101245360A CN 101245360 A CN101245360 A CN 101245360A CN A2008100863797 A CNA2008100863797 A CN A2008100863797A CN 200810086379 A CN200810086379 A CN 200810086379A CN 101245360 A CN101245360 A CN 101245360A
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- paclitaxel
- enzyme hydrolysis
- mairei
- technique
- taxol
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Abstract
The invention relates to a technique for extracting paclitaxel from Taxus chinensis var.mairei by the biological enzyme hydrolysis method, which is characterized in that the branches and leaves of the Taxus chinensis var.mairei are taken as raw materials, a D426 of yeast-like fungi is taken as an enzyme hydrolysis microbe, the enzyme hydrolysis is carried out at 32 to 36 DEG C, and then the paclitaxel with the high content is obtained by instant freezing, high-speed separation and spray drying. The technique carries out the enzyme hydrolysis of the branches and leaves of the Taxus chinensis var.mairei by selecting a specific microbial strain, so as to solve the technical problems of molecular wall-breaking and hydrophobia; the grade one extraction rate is as high as 96 percent, and no organic solvent residue or environmental pollution is generated by the technique.
Description
Technical field
The invention belongs to and from plant, extract the activeconstituents technical field, be specifically related to taxol biologic enzymolysis method extraction process in the southerm yew.
Background technology
Taxol has good anti-cancer activity, especially late period, transitivity ovarian cancer, mammary cancer, lung cancer is had very significant curative effect, and is also effective in cure to cervical cancer, the esophageal carcinoma, cancer of the stomach, bladder cancer, malignant melanoma, malignant lymphoma.The technology of extracting taxus brevifolia alcohol at present mainly contains chemical method and (comprises methods such as solvent soaking, acid degradation, alkaline degradation.The shortcoming of these class methods is often to cause the effective constituent sex change, hazard residue in the product is higher and be difficult to remove), the physics method (uses physical actions such as low temperature, freezing (embrittlement), ultrasonic wave, microwave to destroy the Ganoderma spore walls, shortcoming is that active substance is difficult to extract fully, and apparatus expensive), mechanical process (is destroyed cell walls by rolling, push, spray mechanical effects such as pulverizing, comminution by gas stream, bump, shortcoming jljl logos is the same, but equipment manufacturing cost is lower).
Owing to have the hard cell walls shell of one deck in the yew cell tissue, and show as lipid-soluble substance under the common state of taxol, therefore adopt ordinary method to be difficult to make the abundant broken wall of vegetable cell, and must be with an organic solvent, ubiquity extraction time is long, yield is low, product contains organic substance residues.
Summary of the invention
The purpose of this invention is to provide taxol biologic enzymolysis method extraction process in a kind of southerm yew, this method is abundant to the taxol extraction, extraction efficiency is high, technology is simple, cost is low, non-environmental-pollution.
Extracting method feature of the present invention is to be raw material with southern Ramulus et folium taxi cuspidatae, is the enzymolysis microorganism with the D426 of yeast-like fungi, carries out enzymolysis under 32-36 ℃ of condition, passes through flash freezing, high-speed separation, spraying drying again, obtains the high-content taxol.
Enzymolysis microorganism in the above-mentioned enzymolysis process adds with its active thalline powder, its preparation method places bacterium culture medium slant culture bacterial classification for the D426 with yeast-like fungi, bacterium culture medium is a bouillon agar, then seal up for safekeeping by Valelinum Liquidum, transplant again after 3 months and seal up for safekeeping, strengthen culture medium solution, make it expand the group, get its algae that grows floating on water then and be lyophilized into active thalline powder.
The present invention carries out enzymolysis by selecting specific microorganism strains for use to Ramulus et folium taxi cuspidatae, make the abundant broken wall of vegetable cell, and the hydrophobic group in its molecular structure effectively contained, under the prerequisite that does not change other functional groups, make its possess hydrophilic property matter, thereby can be that solvent directly extracts behind enzymolysis with water, solved the technical barrier of molecule broken wall and hydrophobic nature.Compared with prior art, the one-level extraction yield up to 96%, residual, the non-environmental-pollution of organic solvent-free.
Embodiment
Embodiment 1
Take by weighing 1000 kilograms in branches and leaves that clean the back Ramulus et folium taxi cuspidatae, squeeze the juice through making beating, mix 150 gram microorganism active thalline powder, stir and be to leave standstill 5 days under 32 ℃ of conditions in temperature, moment cryoconcentration to 25 kilogram, obtain powdery dry-matter 755.6 grams through high speed centrifugation separation, spraying drying then, according to Deyu Group Corp. Jiangxi Prov.'s company standard (Q/JDY01-2007) method, the content that records its taxol is 1.63%.
Embodiment 2
Take by weighing 1000 kilograms in branches and leaves that clean the back Ramulus et folium taxi cuspidatae, squeeze the juice through making beating, mix 150 gram microorganism active thalline powder, stir and be to leave standstill 5 days under 34 ℃ of conditions in temperature, moment cryoconcentration to 25 kilogram, obtain powdery dry-matter 798.4 grams through high speed centrifugation separation, spraying drying then, according to Deyu Group Corp. Jiangxi Prov.'s company standard (Q/JDY01-2007) method, the content that records its taxol is 1.53%.
Embodiment 3
Take by weighing 1000 kilograms in branches and leaves that clean the back Ramulus et folium taxi cuspidatae, squeeze the juice through making beating, mix 150 gram microorganism active thalline powder, stir and be to leave standstill 5 days under 36 ℃ of conditions in temperature, moment cryoconcentration to 25 kilogram, obtain powdery dry-matter 853.6 grams through high speed centrifugation separation, spraying drying then, according to Deyu Group Corp. Jiangxi Prov.'s company standard (Q/JDY01-2007) method, the content that records its taxol is 1.42%.
Claims (1)
1, taxol biologic enzymolysis method extraction process in a kind of southerm yew, it is characterized in that with southern Ramulus et folium taxi cuspidatae be raw material, D426 with yeast-like fungi is the enzymolysis microorganism, under 32-36 ℃ of condition, carry out enzymolysis, pass through flash freezing, high-speed separation, spraying drying again, obtain the high-content taxol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100863797A CN101245360A (en) | 2008-03-24 | 2008-03-24 | Biological enzymatic hydrolysis extraction technique of paclitaxel from south yew |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100863797A CN101245360A (en) | 2008-03-24 | 2008-03-24 | Biological enzymatic hydrolysis extraction technique of paclitaxel from south yew |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101245360A true CN101245360A (en) | 2008-08-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100863797A Pending CN101245360A (en) | 2008-03-24 | 2008-03-24 | Biological enzymatic hydrolysis extraction technique of paclitaxel from south yew |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071230A (en) * | 2010-11-23 | 2011-05-25 | 李民生 | Method for extracting paclitaxel from taxus chinensis through microorganism fermentation |
| CN104140689A (en) * | 2014-07-14 | 2014-11-12 | 红豆集团无锡南国企业有限公司 | Method for extracting natural pigments from Chinese yew residue obtained after preparation of medicine |
| CN106675786A (en) * | 2016-08-25 | 2017-05-17 | 江玉成 | Method for extracting essential oil mixture from branches, leaves, bark and root of Chinese yew and Chinese yew essential oil mixture |
| CN113862325A (en) * | 2021-11-09 | 2021-12-31 | 四川贝美森生物科技有限公司 | Taxus chinensis fermentation liquor with high taxol content as well as preparation method and application thereof |
-
2008
- 2008-03-24 CN CNA2008100863797A patent/CN101245360A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071230A (en) * | 2010-11-23 | 2011-05-25 | 李民生 | Method for extracting paclitaxel from taxus chinensis through microorganism fermentation |
| CN104140689A (en) * | 2014-07-14 | 2014-11-12 | 红豆集团无锡南国企业有限公司 | Method for extracting natural pigments from Chinese yew residue obtained after preparation of medicine |
| CN104140689B (en) * | 2014-07-14 | 2016-02-17 | 红豆集团无锡南国企业有限公司 | The method of natural pigment is extracted from Ramulus et folium taxi cuspidatae pharmacy residue |
| CN106675786A (en) * | 2016-08-25 | 2017-05-17 | 江玉成 | Method for extracting essential oil mixture from branches, leaves, bark and root of Chinese yew and Chinese yew essential oil mixture |
| CN106675786B (en) * | 2016-08-25 | 2020-01-24 | 江玉成 | Method for extracting essential oil mixture from branches, leaves, bark and roots of taxus chinensis and taxus chinensis essential oil mixture |
| CN113862325A (en) * | 2021-11-09 | 2021-12-31 | 四川贝美森生物科技有限公司 | Taxus chinensis fermentation liquor with high taxol content as well as preparation method and application thereof |
| CN113862325B (en) * | 2021-11-09 | 2024-04-16 | 四川贝美森生物科技有限公司 | Taxus chinensis fermentation liquor with high taxol content and preparation method and application thereof |
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Open date: 20080820 |