CN106666080A - Microbiological feed additive with multiple effects and preparation method of microbiological feed additive - Google Patents
Microbiological feed additive with multiple effects and preparation method of microbiological feed additive Download PDFInfo
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- CN106666080A CN106666080A CN201510747967.0A CN201510747967A CN106666080A CN 106666080 A CN106666080 A CN 106666080A CN 201510747967 A CN201510747967 A CN 201510747967A CN 106666080 A CN106666080 A CN 106666080A
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Abstract
The invention relates to a microbiological feed additive with multiple effects. The microbiological feed additive is characterized by comprising composite microbe liquor and trace elements, wherein the composite microbe liquor contains bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus, saccharomyces cerevisiae, lactobacillus casei and pediococcus pentosaceus, and the trace elements contain copper, iron, zinc, manganese, iodine, selenium and calcium. A preparation method of the microbiological feed additive with multiple effects comprises the following steps of preparing bacillus subtilis liquor, enterococcus faecalis liquor, lactobacillus acidophilus liquor, saccharomyces cerevisiae liquor, lactobacillus casei liquor and pediococcus pentosaceus liquor; detecting the number of active microbes in the microbe liquor; mixing the microbe liquor in the compounding ratio so as to obtain a composite microbial agent; and weighing the trace elements according to the content of the trace elements, mixing the weighed trace elements with the microbial agent, and performing uniform stirring. According to the microbiological feed additive and the preparation method thereof disclosed by the invention, a microbiological additive is used for improving a poultry feed, so that the eating safety of poultry is guaranteed, trace elements desired by human bodies are complemented, the mouth feel of the poultry is improved, and the microbiological feed additive is simple and convenient and is easy for various breeding households and feed production enterprises to operate.
Description
Technical field
The present invention relates to a kind of livestock fodder additives, particularly a kind of multiple-effect microbial feed addictive and preparation method thereof.
Background technology
With the raising of human living standard, meat is into requisite food of often eating on mankind's dining table, therewith, large-scale poultry farms start gradually to increase, large-scale plant can make poultry easily infect some diseases, and spread speed is fast, scope is wide, therefore the method that Ge great plants solve this problem is exactly to add substantial amounts of antibiotic and all kinds of chemicals in daily feed, this similar drug is very harmful to the mankind, and in large-scale cultivation, bird rarely has activity, plus the quality problem of feed, the meat taste of this poultry is very poor, simultaneously, the mankind are difficult to from these meat foods absorb the nutrient of some needed by human body, such as calcium, iron, selenium and other trace elements.
Poultry lacks selenium, more because lacking selenium in feed and water, such as some regional corns containing selenium be only 0.0057ppm wheats be 0.0046ppm；Soya bean is 0.0067ppm；Well below the demand needed for domestic animal（0.1ppm）, because feed and drinking-water lack selenium, cause livestock and poultry physiological function that obstacle occurs, make livestock and poultry show different scarce selenium symptoms.Pig lacks selenium and mulberries sample cardiomyopathy occurs, and more lipid occurs in cardiac muscle cell, and the extensive bleeding of heart simultaneously trophism hepatonecrosis occurs.There is white muscle disease in calf and lamb, the myofibrosis of skeletal muscle because lacking selenium, connective tissue proliferation, white striped.Ill domestic animal four limbs are stiff, shake, with disordered breathing-, body temperature rises.Can also result in ewe infertile, the card shape such as milk cow miscarriage, diarrhoea.Broiler chicken lacks selenium, and cellular exudate is accumulated in the muscle gap of subcutaneous abdomen and tissue, visually can be seen that the viscous liquid in blue, and growth retardation, the death rate is higher.Laying hen lacks selenium affects egg production and incubation rate.Poultry calcium deficiency, lacks calcareous in mainly internal calcium loss and food, equally, poultry iron deficiency also in that food, therefore, the present invention provides a kind of additive for microbe feedstuff of safety and Health to solve problem above.
The content of the invention
The technical problem to be solved in the present invention is, for the deficiencies in the prior art, to propose a kind of multiple-effect microbial feed addictive and preparation method thereof.
The technical problem to be solved in the present invention is achieved through the following technical solutions.The present invention is a kind of multiple-effect microbial feed addictive, is characterized in：Including the bacterium solution and trace element of compound bacteria, there are bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus, saccharomyces cerevisiae, Lactobacillus casei, Pediococcus pentosaceus in the bacterium solution of the compound bacteria, the trace element is copper, iron, zinc, manganese, iodine, selenium and calcium.
In a kind of multiple-effect microbial feed addictive of the present invention, further preferred technical scheme is characterized in that：
1st, the weight ratio of each component is bacillus subtilis in the bacterium solution of the compound bacteria：Enterococcus faecalis：Lactobacillus acidophilus：Saccharomyces cerevisiae：Lactobacillus casei：Pediococcus pentosaceus=0.5-0.8：0.3-0.6：0.4：0.6：0.2-0.4:0.1-0.3；
2nd, the g/ mL of the trace copper 2, the g/ mL of iron 4, zinc 5g/ mL, manganese 5g/ mL, iodine 1g/ mL, the 0g/ mL of selenium 1 and the 0g/ mL of calcium 2；
3rd, in the bacterium solution of the compound bacteria, bacillus subtilis viable count is 2.0 × 109 CFU/mL-3×109CFU/mL, enterococcus faecalis liquid viable bacteria are 1.0 × 109 CFU/mL-1.5×109CFU/mL, lactobacillus acidophilus viable count are 1.0 × 109 CFU/mL-2.0×109CFU/mL, saccharomyces cerevisiae viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Lactobacillus casei viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Pediococcus pentosaceus viable count are 1.0 × 109 CFU/mL-1.5×109CFU/mL；
A kind of 4 preparation methods of multiple-effect microbial feed addictive, step is as follows：
（1）Prepare bacillus subtilis bacterium solution, enterococcus faecalis liquid, lactobacillus acidophilus liquid, saccharomyces cerevisiae liquid, Lactobacillus casei liquid, Pediococcus pentosaceus liquid；
（2）Active bacteria quantity in detection bacterium solution, when bacillus subtilis viable count is 2.0 × 109 CFU/mL-3×109CFU/mL, enterococcus faecalis liquid viable bacteria are 1.0 × 109 CFU/mL-1.5×109CFU/mL, lactobacillus acidophilus viable count are 1.0 × 109 CFU/mL-2.0×109CFU/mL, saccharomyces cerevisiae viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Lactobacillus casei viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Pediococcus pentosaceus viable count are 1.0 × 109 CFU/mL-1.5×109During CFU/mL, each bacterium is also taken out into stand-by；
（3）Each bacterium solution is prepared by mixing into complex micro organism fungicide according to said ratio；
（4）Content according to above-mentioned trace element weighs each trace element, mixes into complex micro organism fungicide, stirs evenly.
Compared with prior art, by the present invention in that poultry is raised with microbe additive improveing, ensure that the safety of poultry food, supplement the trace element of needed by human body, improve the mouthfeel of poultry, can effectively suppress a large amount of miscellaneous bacterias, the growth and breeding of harmful bacteria contained in feeding system, feedstuff, it is simple and convenient, it is easy to all kinds of raisers, production Feed Enterprise operation, the green safety of food is also ensure that while poultry disease propagation is resisted.
Specific embodiment
The concrete technical scheme of the present invention is further described, in order to those skilled in the art the present invention is further understood that, and do not constitute the restriction of its power.
Embodiment 1, the present invention is a kind of multiple-effect microbial feed addictive, is characterized in：Including the bacterium solution and trace element of compound bacteria, there are bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus, saccharomyces cerevisiae, Lactobacillus casei, Pediococcus pentosaceus in the bacterium solution of the compound bacteria, the trace element is copper, iron, zinc, manganese, iodine, selenium and calcium.
Embodiment 2, a kind of multiple-effect microbial feed addictive described in embodiment 1, the present invention is a kind of multiple-effect microbial feed addictive, and the weight ratio of each component is bacillus subtilis in the bacterium solution of the compound bacteria：Enterococcus faecalis：Lactobacillus acidophilus：Saccharomyces cerevisiae：Lactobacillus casei：Pediococcus pentosaceus=0.5-0.8：0.3-0.6：0.4：0.6：0.2-0.4:0.1-0.3；
Embodiment 3, a kind of multiple-effect microbial feed addictive described in embodiment 1, the g/ mL of the trace copper 2, the g/ mL of iron 4, zinc 5g/ mL, manganese 5g/ mL, iodine 1g/ mL, the 0g/ mL of selenium 1 and the 0g/ mL of calcium 2；
Embodiment 4, a kind of multiple-effect microbial feed addictive described in embodiment 1, in the bacterium solution of the compound bacteria, bacillus subtilis viable count is 2.0 × 109 CFU/mL-3×109CFU/mL, enterococcus faecalis liquid viable bacteria are 1.0 × 109 CFU/mL-1.5×109CFU/mL, lactobacillus acidophilus viable count are 1.0 × 109 CFU/mL-2.0×109CFU/mL, saccharomyces cerevisiae viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Lactobacillus casei viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Pediococcus pentosaceus viable count are 1.0 × 109 CFU/mL-1.5×109 CFU/mL。
A kind of embodiment 5, preparation method of multiple-effect microbial feed addictive, step is as follows：
（1）Prepare bacillus subtilis bacterium solution, enterococcus faecalis liquid, lactobacillus acidophilus liquid, saccharomyces cerevisiae liquid, Lactobacillus casei liquid, Pediococcus pentosaceus liquid；
（2）Active bacteria quantity in detection bacterium solution, when bacillus subtilis viable count is 2.0 × 109 CFU/mL-3×109CFU/mL, enterococcus faecalis liquid viable bacteria are 1.0 × 109 CFU/mL-1.5×109CFU/mL, lactobacillus acidophilus viable count are 1.0 × 109 CFU/mL-2.0×109CFU/mL, saccharomyces cerevisiae viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Lactobacillus casei viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Pediococcus pentosaceus viable count are 1.0 × 109 CFU/mL-1.5×109During CFU/mL, each bacterium is also taken out into stand-by；
（3）Each bacterium solution is prepared by mixing into complex micro organism fungicide according to said ratio；
（4）Content according to above-mentioned trace element weighs each trace element, mixes into complex micro organism fungicide, stirs evenly.
Claims (5)
1. a kind of multiple-effect microbial feed addictive, it is characterised in that：Including the bacterium solution and trace element of compound bacteria, there are bacillus subtilis, enterococcus faecalis, lactobacillus acidophilus, saccharomyces cerevisiae, Lactobacillus casei, Pediococcus pentosaceus in the bacterium solution of the compound bacteria, the trace element is copper, iron, zinc, manganese, iodine, selenium and calcium.
2. multiple-effect microbial feed addictive according to claim 1, it is characterised in that：The weight ratio of each component is bacillus subtilis in the bacterium solution of the compound bacteria：Enterococcus faecalis：Lactobacillus acidophilus：Saccharomyces cerevisiae：Lactobacillus casei：Pediococcus pentosaceus=0.5-0.8：0.3-0.6：0.4：0.6：0.2-0.4:0.1-0.3.
3. multiple-effect microbial feed addictive according to claim 1, it is characterised in that：The g/ mL of the trace copper 2, the g/ mL of iron 4, zinc 5g/ mL, manganese 5g/ mL, iodine 1g/ mL, the 0g/ mL of selenium 1 and the 0g/ mL of calcium 2.
4. multiple-effect microbial feed addictive according to claim 1, it is characterised in that：In the bacterium solution of the compound bacteria, bacillus subtilis viable count is 2.0 × 109 CFU/mL-3×109CFU/mL, enterococcus faecalis liquid viable bacteria are 1.0 × 109 CFU/mL-1.5×109CFU/mL, lactobacillus acidophilus viable count are 1.0 × 109 CFU/mL-2.0×109CFU/mL, saccharomyces cerevisiae viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Lactobacillus casei viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Pediococcus pentosaceus viable count are 1.0 × 109 CFU/mL-1.5×109 CFU/mL。
5. a kind of preparation method of multiple-effect microbial feed addictive, it is characterised in that step is as follows：
Prepare bacillus subtilis bacterium solution, enterococcus faecalis liquid, lactobacillus acidophilus liquid, saccharomyces cerevisiae liquid, Lactobacillus casei liquid, Pediococcus pentosaceus liquid；
（2）Active bacteria quantity in detection bacterium solution, when bacillus subtilis viable count is 2.0 × 109 CFU/mL-3×109CFU/mL, enterococcus faecalis liquid viable bacteria are 1.0 × 109 CFU/mL-1.5×109CFU/mL, lactobacillus acidophilus viable count are 1.0 × 109 CFU/mL-2.0×109CFU/mL, saccharomyces cerevisiae viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Lactobacillus casei viable count are 2.0 × 109 CFU/mL-2.5×109CFU/mL, Pediococcus pentosaceus viable count are 1.0 × 109 CFU/mL-1.5×109During CFU/mL, each bacterium is also taken out into stand-by；
（3）Each bacterium solution is prepared by mixing into complex micro organism fungicide according to said ratio；
（4）Content according to above-mentioned trace element weighs each trace element, mixes into complex micro organism fungicide, stirs evenly.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108208325A (en) * | 2018-01-19 | 2018-06-29 | 深圳市帝之道科技贸易有限公司 | A kind of probiotics composite mineral matter additive |
| CN109874921A (en) * | 2019-04-11 | 2019-06-14 | 扬州大学 | A kind of microbiological feed and preparation method thereof for Procambius clarkii cultivation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455278A (en) * | 2007-12-17 | 2009-06-17 | 山东六和集团有限公司 | Big-broiler feedstuff and preparation method thereof |
| CN104621355A (en) * | 2014-11-17 | 2015-05-20 | 德安敷阳综合养殖场有限公司 | Herbal nutritious chicken feed additive and preparation method thereof |
-
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- 2015-11-06 CN CN201510747967.0A patent/CN106666080A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455278A (en) * | 2007-12-17 | 2009-06-17 | 山东六和集团有限公司 | Big-broiler feedstuff and preparation method thereof |
| CN104621355A (en) * | 2014-11-17 | 2015-05-20 | 德安敷阳综合养殖场有限公司 | Herbal nutritious chicken feed additive and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108208325A (en) * | 2018-01-19 | 2018-06-29 | 深圳市帝之道科技贸易有限公司 | A kind of probiotics composite mineral matter additive |
| CN109874921A (en) * | 2019-04-11 | 2019-06-14 | 扬州大学 | A kind of microbiological feed and preparation method thereof for Procambius clarkii cultivation |
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