CN106635806A - Formula of skeletonema costatum greville medium - Google Patents
Formula of skeletonema costatum greville medium Download PDFInfo
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- CN106635806A CN106635806A CN201510731377.9A CN201510731377A CN106635806A CN 106635806 A CN106635806 A CN 106635806A CN 201510731377 A CN201510731377 A CN 201510731377A CN 106635806 A CN106635806 A CN 106635806A
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- skeletonema costatum
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- greville
- culture medium
- skeletonema
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Abstract
The invention relates to the field of marine micro-algae breeding, and in particular to a formula of a skeletonema costatum greville medium. Ingredients and contents of the formula are as follows: 80-120mg/L of ammonium bicarbonate, 5-15mg/L of monopotassium phosphate, 5-15mg/L of dipotassium phosphate, 0.5-1.5mg/L of ammonium ferric citrate, 15-25mg/L of sodium metasilicate nonahydrate, 0.1-1mg/L of naphthalene acetamide, 1ml/L of polydimethylsiloxane and 10 ml/L of a soil extracting solution. According to the formula, a nitrogen source and a carbon source are provided for skeletonema costatum greville from sodium bicarbonate; by virtue of the naphthalene acetamide and the polydimethylsiloxane, cell division and absorption are promoted; and by replacing trace elements with the soil extracting solution, the growth rate of algae is improved and cell density is increased, and in addition, production cost is greatly reduced.
Description
Technical field
The present invention relates to field of ocean microalgae culture, more particularly to a kind of Skeletonema Costatum culture medium prescription.
Background technology
Skeletonema Costatum (Skeletonema costatum Greville) be Bacillariophyta, rotary strainer Cutleriales, Skeletonema Greville section,
A kind of wide temperature of Skeletonema, eurysalinity marine diatom class, with it is nutritious, palatability is good the features such as, by extensively should
For shrimps, shellfish, fish seedling stage bait.But because its cultivate difficulty it is big and and do not form the scale of factorial praluction,
It is very big to the demand of Skeletonema Costatum that the coastal each aquatic fry growing in the whole nation cultivates unit.At present, the master of Skeletonema Costatum culture
Problem is wanted to be frustule poor growth, plateau algae cell density is low, easily pollute.
For Skeletonema Costatum culture medium, the past, people thought always, the CO dissociated in water body2It is that algae utilizes the external world
The unique forms of DIC.Part scientist research later has shown that, HCO3 -Can also be absorbed by Skeletonema Costatum as carbon source
Utilize.And nitrogen source is often used as in culture medium is nitrate, research has shown that, algae absorbs the optimum of nitrogen source
Form is ammonium salt.
NAD, molecular formula C10H7CH2CONH2, it is a plant growth regulators, cell division can be promoted.Poly- diformazan
Radical siloxane is a kind of good chelating agent, can promote absorption of the frustule to nutrient.
The content of the invention
In order to solve the problems, such as prior art, a kind of Skeletonema Costatum culture medium prescription is we provided.
The concrete technical scheme of the present invention is as follows：
A kind of Skeletonema Costatum culture medium prescription, formula components and content it is as follows：80～120mg/L of ammonium hydrogen carbonate, phosphoric acid
The water sodium metasilicate of 5～15mg/L of potassium dihydrogen, 5～15mg/L of dipotassium hydrogen phosphate, 0.5～1.5mg/L of ferric citrate, nine 15～
25mg/L, 0.1～1mg/L of NAD, dimethyl silicone polymer 1ml/L, soil extract 10ml/L.
The NAD is made into mother liquor and uses, and is placed in 4 DEG C of Refrigerator stores.
The beneficial effect that the present invention brings：
1st, using ammonium hydrogen carbonate as main nutrient composition, while providing nitrogen source and carbon source for Skeletonema Costatum so as to more
Easily absorb nutriment.
2nd, using NAD as plant growth regulator, frustule can be promoted to divide；Made using dimethyl silicone polymer
For chelating agent, absorption of the frustule to nutriment can be promoted, so as to improve growth rate, increase cell density.
3rd, using soil extract Substituting Some Trace Elements solution, production cost is not only reduced, moreover it is possible to provide various micro
Element, promotes frustule growth.
Specific embodiment
In conjunction with specific embodiment, the present invention will be described in detail：
Embodiment 1
A kind of Skeletonema Costatum culture medium prescription, formula components and content it is as follows：Ammonium hydrogen carbonate 80mg/L, potassium dihydrogen phosphate
5mg/L, dipotassium hydrogen phosphate 5mg/L, ferric citrate 0.5mg/L, nine water sodium metasilicate 15mg/L, NAD 0.1mg/L,
Dimethyl silicone polymer 1ml/L, soil extract 10ml/L.
Embodiment 2
A kind of Skeletonema Costatum culture medium prescription, formula components and content it is as follows：Ammonium hydrogen carbonate 120mg/L, biphosphate
The water sodium metasilicate 25mg/L of potassium 15mg/L, dipotassium hydrogen phosphate 15mg/L, ferric citrate 1.5mg/L, nine, NAD
1mg/L, dimethyl silicone polymer 1ml/L, soil extract 10ml/L.
Embodiment 3
A kind of Skeletonema Costatum culture medium prescription, formula components and content it is as follows：Ammonium hydrogen carbonate 100mg/L, biphosphate
Potassium 10mg/L, dipotassium hydrogen phosphate 10mg/L, ferric citrate 1mg/L, nine water sodium metasilicate 20mg/L, NAD 0.5mg/L,
Dimethyl silicone polymer 1ml/L, soil extract 10ml/L.
Using f/2 culture mediums as control, the Skeletonema Costatum algae solution of identical initial density is accessed, determine its growth curve.
Experimental result shows that the growth rate of algae solution is embodiment 2 in four groups of culture mediums>Embodiment 3>Embodiment 1>F/2 culture mediums.
Illustrate that the present invention can improve the growth rate of Skeletonema Costatum, increase cell density.
Above are only a specific embodiment of the present invention, it is clear that the invention is not restricted to above example, can also have
Many deformations.All deformations that one of ordinary skill in the art can directly derive from present disclosure or associate,
It is considered as protection scope of the present invention.
Claims (2)
1. a kind of Skeletonema Costatum culture medium prescription, it is characterised in that the formula components and content of the culture medium are as follows：
80～120mg/L of ammonium hydrogen carbonate, 5～15mg/L of potassium dihydrogen phosphate, 5～15mg/L of dipotassium hydrogen phosphate, ferric citrate 0.5～
1.5mg/L, nine water 15～25mg/L of sodium metasilicate, 0.1～1mg/L of NAD, dimethyl silicone polymer 1ml/L, soil
Earth extract 10ml/L.
2. Skeletonema Costatum culture medium prescription according to claim 1, it is characterised in that the NAD is made into mother
Liquid is used, and is placed in 4 DEG C of Refrigerator stores.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510731377.9A CN106635806A (en) | 2015-10-30 | 2015-10-30 | Formula of skeletonema costatum greville medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510731377.9A CN106635806A (en) | 2015-10-30 | 2015-10-30 | Formula of skeletonema costatum greville medium |
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| Publication Number | Publication Date |
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| CN106635806A true CN106635806A (en) | 2017-05-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201510731377.9A Pending CN106635806A (en) | 2015-10-30 | 2015-10-30 | Formula of skeletonema costatum greville medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795839A (en) * | 2018-07-02 | 2018-11-13 | 中国科学院成都生物研究所 | A kind of method of stem of noble dendrobium single-cell suspension culture |
-
2015
- 2015-10-30 CN CN201510731377.9A patent/CN106635806A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795839A (en) * | 2018-07-02 | 2018-11-13 | 中国科学院成都生物研究所 | A kind of method of stem of noble dendrobium single-cell suspension culture |
| CN108795839B (en) * | 2018-07-02 | 2022-02-11 | 中国科学院成都生物研究所 | Method for suspension culture of dendrobium single cells |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170510 |