CN106622410A - Ultrasonic sealing method based on laser recasting object micro-energy-oriented ridge - Google Patents
Ultrasonic sealing method based on laser recasting object micro-energy-oriented ridge Download PDFInfo
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- CN106622410A CN106622410A CN201611138289.9A CN201611138289A CN106622410A CN 106622410 A CN106622410 A CN 106622410A CN 201611138289 A CN201611138289 A CN 201611138289A CN 106622410 A CN106622410 A CN 106622410A
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- ultrasonic sealing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
Abstract
The invention provides an ultrasonic sealing method based on a laser recasting object micro-energy-oriented ridge and belongs to the technical field of ultrasonic sealing of thermoplastic polymer micro-fluidic chips. A micro-convex structure is produced on the edge of a groove formed after a thermoplastic polymer micro-fluidic chip is subjected to laser processing, and the method is used for performing ultrasonic sealing by the structure. The ultrasonic sealing method based on the laser recasting object micro-energy-oriented ridge comprises the steps of preparing a required micro-channel structure on a thermoplastic polymer substrate by a hot-pressing or injection-molding method; positioning the substrate and a laser head; performing laser processing along the periphery of a micro-structure which needs to be sealed to form the recasting object micro-energy-oriented ridge; and putting the substrate and a corresponding cover sheet in ultrasonic sealing equipment and performing ultrasonic sealing. The ultrasonic sealing method based on the laser recasting object micro-energy-oriented ridge is simple and flexible in process and high in sealing quality; and the sealed micro-fluidic chip can be applied in the fields of medicine screening, disease intermediate detection and the like.
Description
Technical field
The invention belongs to thermoplastic polymer micro-fluidic chip ultrasound sealing technical field, and in particular to one kind is based on laser
The ultrasonic sealing method of the micro- energy-oriented-ridge of recast.
Background technology
Micro-fluidic chip based on microflow control technique, after early 1990s are suggested, just receives more
Carry out more concerns.Micro-fluidic chip can concentrate the mixing in biochemical analysis, and it is flat that the operation such as dilution and separation is integrated into number
On the chip of square centimetre of size.With small volume, integrated and high degree of automation, analysis efficiency and high precision, sample and examination
The advantages of agent consumption is few.Obtain in the field such as detection immediately in water pollution monitoring, drug screening, cell control sorting and disease
Extensive application is arrived.Thermoplastic, polymeric materials are due to its low cost, it is easy to be molded, it is easy to accomplish the advantage such as mass production,
The main material of manufacture micro-fluidic chip is become gradually.
Thermoplastic polymer micro-fluidic chip is typically made up of at least double-layer structure, therefore the sealing of chip is that it was manufactured
An indispensable step in journey.Now the most frequently used thermoplastic polymer micro-fluidic chip sealing method is heat sealing and sticker envelope
Close.Wherein, the temperature required height of heat sealing, easily causes the biological reagent inactivation in chip.And heat sealing efficiency is low, during sealing
Between more than 20 minutes.Moreover, due to apply higher temperature and pressure during heat sealing, so chip microchannel
Easily by compression.Sticker sealing just can operate at room temperature, but glued membrane used easily with chip in biological reagent
Produce bio-compatible sex chromosome mosaicism.And glued membrane easily blocks chip microchannel.
Ultrasound sealing by contrast has some superiority.Ultrasonic energy concentrates on and is referred to as leading energy when first ultrasound is sealed
In the microprotrusion of muscle.With a certain distance from having from microchannel due to energy-oriented-ridge, therefore the heat for producing will not be to the biology in microchannel
Reagent produces impact.Secondly as ultrasound sealing in do not need other intermediate mediums, therefore will not produce bio-compatibility and in
Between dielectrically inhibited microchannel problem.Finally, ultrasound sealing efficiency high, just can complete the envelope to micro-fluidic chip in one minute
Close.And ultrasonic sealing strength is high, can reach the strength of materials of thermoplastic polymer itself, this is for needing high-pressure work ring
The micro-fluidic chip in border is significant.
However, ultrasonic sealing technology at this stage still suffers from some problems.Wherein most distinct issues are ultrasound sealing essences
Degree is not high.The reason for causing this problem is that energy-oriented-ridge can occur melt-flow under ultrasonic energy effect in ultrasound sealing,
The energy-oriented-ridge of melting easily flows into microchannel and causes obstruction, or leaves a void so as to cause reagent to leak on sealing interface.
Now most solution is to manufacture and design special molten bath structure by energy-oriented-ridge, so that the energy-oriented-ridge of melting is flowed into
To in the structure of molten bath.But under minute yardstick, energy-oriented-ridge and molten bath manufacture and design complexity, and need according to different micro-fluidic
Chip structure is customized.A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast proposed by the present invention can be solved
This problem, it is significant for the sealing of micro-fluidic chip high-precision ultrasound.
Technical scheme
It is an object of the invention to solve energy-oriented-ridge and molten bath structure design manufacture difficulty under minute yardstick, energy-oriented-ridge stream is melted
Move uppity problem.
Technical scheme：
A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast, step is as follows：
(1) micro-fluidic chip is manufactured and designed according to demand, the micro-fluidic chip is made up of cover plate 1 and substrate 4；
(2) substrate 4 and laser head are positioned, first, on Laser Processing platform thermoplastic polymer flat board is fixed
As alignment surface, area of its area more than micro-fluidic chip to be sealed；Then, the micro-fluidic chip for step (1) being designed
Substrate structure is imported in laser-processing system, and according to micro-fluidic chip substrate structure a laser scanning is carried out, by the miniflow
Control chip substrate structure replication is on alignment surface；Finally, by the micro-fluidic chip substrate structure obtained in step (1) be aligned
The replicated figure for obtaining is aligned in plane, till the two is completely superposed；Using laser head coordinate now as base
Keep the coordinate and micro-fluidic chip substrate position constant in quasi coordinates, and operating procedure afterwards；
(3) the micro- energy-oriented-ridge layout of recast is carried out, laser power, laser scanning speed and laser scanning number of times, edge is adjusted
The periphery of mixing pit 7, comb-tooth-type delayer 8 and reaction tank 10 carries out laser scanning manufacturing on substrate 4, forms that recast is micro- to lead energy
Muscle 5 and molten bath 11；Microchannel corner without the micro- energy-oriented-ridge 5 of recast, as white space 6, the length of white space 6 is
1-200μm；The height of the micro- energy-oriented-ridge 5 of recast is 10-100 μm；The micro- energy-oriented-ridge 5 of recast is from the spacing 9 of microchannel outer wall
10-2000μm；
(4) substrate 4 obtained in above-mentioned steps and cover plate 1 are placed in ultrasound sealing fixture carries out alignment fixation, applies
After certain ultrasound sealing parameter, micro-fluidic chip is taken out from ultrasound sealing fixture, complete ultrasound envelope proposed by the present invention
Conjunction process；Wherein, ultrasonic sealing pressure is 0.1-1MPa, and the ultrasonic vibration time is 0.01-1s, and ultrasonic amplitude is 10-100 μm.
Material used by micro-fluidic chip be polymethyl methacrylate, polyethylene terephthalate, polytetrafluoroethylene (PTFE),
Merlon, polystyrene, polyethylene, polypropylene, polyvinyl chloride, nylon, polyurethane or styrene methyl methacrylate
Copolymer.
When laser is scanned on thermoplastic polymer, due to the effect of thermal stress, understands some material and be extruded,
The slot wedge gone out in laser ablation forms microprotrusion structure.The microprotrusion structure is referred to as in the present invention that recast is micro- to lead
Can muscle.The present invention carries out ultrasonic sealing using the recast structure, and the recast of melting can be flowed into the groove for laser machining out.
The groove of Laser Processing serves the effect in molten bath.So through once laser machining, energy-oriented-ridge and molten bath will produce simultaneously.
Eliminate the Design and manufacturing process of complexity.
Beneficial effects of the present invention：The simple laser scanning manufacturing of ultrasonic sealing method Jing proposed by the present invention just can be formed
Micro- energy-oriented-ridge and molten bath structure, it is not necessary to the energy-oriented-ridge and molten bath Design and manufacturing process of complexity.Recast proposed by the present invention is micro-
Energy-oriented-ridge, it highly can be adjusted by laser power, laser scanning speed and laser scanning number of times.It is proposed by the present invention
The layout designs of the micro- energy-oriented-ridge of recast can be completed by mapping software, convenient flexible.Finally, it is proposed by the present invention
A kind of method sealing high precision based on the micro- recast ultrasound sealing of laser, sealing strength is high, disclosure satisfy that micro-fluidic chip enters
The needs of row biochemistry detection.
Description of the drawings
Fig. 1 is the microfluidic chip structure figure sealed using this method.
Fig. 2 is sectional views of the Fig. 1 at A-A.
In figure：1 cover plate；2 liquid storage tanks；3 waste liquid pools；4 substrates；The micro- energy-oriented-ridge of 5 recasts；
6 around the corner without the white space of the micro- energy-oriented-ridge of recast；7 mixing pits；8 comb-tooth-type delayers；
The micro- energy-oriented-ridge of 9 recasts and microchannel outer wall spacing；10 reaction tanks；The molten bath that 11 laser ablations go out.
Specific embodiment
Below in conjunction with accompanying drawing and technical scheme, the specific embodiment of the present invention is further illustrated.
A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast proposed by the present invention is applied to micro- shown in Fig. 1
In fluidic chip.The micro-fluidic chip is made up of cover plate 1 and substrate 4.There are liquid storage tank 2 and waste liquid pool 3 on cover plate.In substrate 4
On have the molten bath 11 that mixing pit 7, comb-tooth-type delayer 8, the micro- energy-oriented-ridge of reaction tank 10, recast (5) and laser ablation go out.Specifically
Sealing operation step is as follows：
(1) pressure sintering is utilized, the mixing pit 7, (application number of comb-tooth-type delayer 8 is processed on substrate 4：
) and reaction tank 10 CN201610566746.8；Using milling method, liquid storage tank 2 and waste liquid pool 3 are processed on cover plate 1；
(2) substrate 4 and laser head are positioned, first, on Laser Processing platform thermoplastic polymer flat board is fixed
As alignment surface, area of its area more than micro-fluidic chip to be sealed；Then, the micro-fluidic chip for step (1) being designed
Substrate structure is imported in laser-processing system, and according to micro-fluidic chip substrate structure a laser scanning is carried out, by the miniflow
Control chip substrate structure replication is on alignment surface；Finally, by the micro-fluidic chip substrate structure obtained in step (1) be aligned
The replicated figure for obtaining is aligned in plane, till the two is completely superposed；Using laser head coordinate now as base
Keep the coordinate and micro-fluidic chip substrate position constant in quasi coordinates, and operating procedure afterwards；
(3) the micro- energy-oriented-ridge layout designs of recast are carried out, laser power is set into 13W, laser scanning speed and is set to
6mm/s, laser scanning number of times are set to 3 times, and the periphery of mixing pit 7, comb-tooth-type delayer 8 and reaction tank 10 is entered along substrate 4
Row laser scanning manufacturing, forms the micro- energy-oriented-ridge 5 of recast and molten bath 11；The height of the micro- energy-oriented-ridge 5 of recast is 51 μm；Recast
Micro- energy-oriented-ridge 5 is 200 μm from the spacing 9 of microchannel outer wall；Microchannel corner without the micro- energy-oriented-ridge 5 of recast, it is as empty
White region 6, the length of white space 6 is 20 μm.
(4) substrate 4 obtained in above-mentioned steps and cover plate 1 are placed in ultrasound sealing fixture carries out alignment fixation, is applying
Plus after the ultrasonic sealing pressure of 0.3MPa, the ultrasonic sealing time of 0.07s and 40 μm of ultrasonic amplitude, by micro-fluidic chip from super
Take out in sound sealing fixture, complete ultrasonic sealing process proposed by the present invention.
Claims (8)
1. a kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast, it is characterised in that step is as follows：
(1) micro-fluidic chip is manufactured and designed according to demand, the micro-fluidic chip is made up of cover plate (1) and substrate (4)；
(2) substrate (4) is positioned with laser head, first, fixes thermoplastic polymer flat board on Laser Processing platform and make
For alignment surface, area of its area more than micro-fluidic chip to be sealed；Then, the micro-fluidic chip base for step (1) being designed
Chip architecture is imported in laser-processing system, and according to micro-fluidic chip substrate structure a laser scanning is carried out, and this is micro-fluidic
Chip substrate structure replication is on alignment surface；Finally, the micro-fluidic chip substrate structure obtained in step (1) is put down with being aligned
The replicated figure for obtaining is aligned on face, till the two is completely superposed；Using laser head coordinate now as benchmark
Keep the coordinate and micro-fluidic chip substrate position constant in coordinate, and operating procedure afterwards；
(3) the micro- energy-oriented-ridge layout of recast is carried out, laser power, laser scanning speed and laser scanning number of times is adjusted, along substrate
(4) periphery of mixing pit (7), comb-tooth-type delayer (8) and reaction tank (10) carries out laser scanning manufacturing on, forms recast micro-
Energy-oriented-ridge (5) and molten bath (11)；Microchannel corner without the micro- energy-oriented-ridge of recast (5), as white space (6)；
(4) substrate obtained in above-mentioned steps (4) and cover plate (1) are placed in ultrasound sealing fixture carries out alignment fixation, applies
After certain ultrasound sealing parameter, micro-fluidic chip is taken out from ultrasound sealing fixture, complete ultrasound envelope proposed by the present invention
Conjunction process；Wherein, ultrasonic sealing pressure is 0.1-1MPa, and the ultrasonic vibration time is 0.01-1s, and ultrasonic amplitude is 10-100 μm.
2. ultrasonic sealing method according to claim 1, it is characterised in that the length of described white space (6) is 1-
200μm。
3. ultrasonic sealing method according to claim 1 and 2, it is characterised in that the micro- energy-oriented-ridge of described recast (5)
Highly it is 10-100 μm.
4. ultrasonic sealing method according to claim 1 and 2, it is characterised in that the micro- energy-oriented-ridge of described recast (5) from
The spacing (9) of microchannel outer wall is 10-2000 μm.
5. ultrasonic sealing method according to claim 3, it is characterised in that the micro- energy-oriented-ridge of described recast (5) is from micro-
The spacing (9) of channel outer wall is 10-2000 μm.
6. the ultrasonic sealing method according to claim 1,2 or 5, it is characterised in that the material used by micro-fluidic chip is
Polymethyl methacrylate, polyethylene terephthalate, polytetrafluoroethylene (PTFE), Merlon, polystyrene, polyethylene, poly- third
Alkene, polyvinyl chloride, nylon, polyurethane or styrene methyl methacrylate copolymer.
7. ultrasonic sealing method according to claim 3, it is characterised in that the material used by micro-fluidic chip is poly- methyl
Methyl acrylate, polyethylene terephthalate, polytetrafluoroethylene (PTFE), Merlon, polystyrene, polyethylene, polypropylene, polychlorostyrene
Ethene, nylon, polyurethane or styrene methyl methacrylate copolymer.
8. ultrasonic sealing method according to claim 4, it is characterised in that the material used by micro-fluidic chip is poly- methyl
Methyl acrylate, polyethylene terephthalate, polytetrafluoroethylene (PTFE), Merlon, polystyrene, polyethylene, polypropylene, polychlorostyrene
Ethene, nylon, polyurethane or styrene methyl methacrylate copolymer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611138289.9A CN106622410B (en) | 2016-12-12 | 2016-12-12 | A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast |
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| CN201611138289.9A CN106622410B (en) | 2016-12-12 | 2016-12-12 | A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast |
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| CN106622410A true CN106622410A (en) | 2017-05-10 |
| CN106622410B CN106622410B (en) | 2018-08-21 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111958117A (en) * | 2020-08-26 | 2020-11-20 | 上海新潮雷特汽车科技有限公司 | Rib making method for shaft |
| CN113058671A (en) * | 2021-04-20 | 2021-07-02 | 中国人民解放军军事科学院军事医学研究院 | Manufacturing method of energy guiding rib for bonding of micro-fluidic chip |
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| CN102756474A (en) * | 2012-07-27 | 2012-10-31 | 苏州大学 | Bonding device for polymer microfluidic chip |
| CN105109034A (en) * | 2015-09-25 | 2015-12-02 | 大连理工大学 | Joint structure used for precise ultrasonic welding of POCT chip products |
| CN105833924A (en) * | 2016-03-16 | 2016-08-10 | 北京同方生物芯片技术有限公司 | Ultrasonic-bonded micro-fluidic chip and preparation method thereof |
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2016
- 2016-12-12 CN CN201611138289.9A patent/CN106622410B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060153741A1 (en) * | 2002-11-15 | 2006-07-13 | Tamata-Tlo Corporation | Micro fluidic control device and process for producing the same |
| CN101100107A (en) * | 2007-05-25 | 2008-01-09 | 江苏大学 | Laser plastic micro-welding method |
| CN102756474A (en) * | 2012-07-27 | 2012-10-31 | 苏州大学 | Bonding device for polymer microfluidic chip |
| CN105109034A (en) * | 2015-09-25 | 2015-12-02 | 大连理工大学 | Joint structure used for precise ultrasonic welding of POCT chip products |
| CN105833924A (en) * | 2016-03-16 | 2016-08-10 | 北京同方生物芯片技术有限公司 | Ultrasonic-bonded micro-fluidic chip and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111958117A (en) * | 2020-08-26 | 2020-11-20 | 上海新潮雷特汽车科技有限公司 | Rib making method for shaft |
| CN113058671A (en) * | 2021-04-20 | 2021-07-02 | 中国人民解放军军事科学院军事医学研究院 | Manufacturing method of energy guiding rib for bonding of micro-fluidic chip |
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| CN106622410B (en) | 2018-08-21 |
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