CN106622410B - A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast - Google Patents
A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast Download PDFInfo
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- CN106622410B CN106622410B CN201611138289.9A CN201611138289A CN106622410B CN 106622410 B CN106622410 B CN 106622410B CN 201611138289 A CN201611138289 A CN 201611138289A CN 106622410 B CN106622410 B CN 106622410B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
Abstract
The present invention provides a kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast, belongs to thermoplastic polymer micro-fluidic chip ultrasound sealing technical field.Microprotrusion structure, the method for carrying out ultrasonic sealing using the structure can be generated when laser machining thermoplastic polymer micro-fluidic chip in the slot wedge processed.First with the method for hot pressing or injection molding required microchannel structure is prepared on thermoplastic polymer substrate；Then the substrate is positioned with laser head；Then, along needing the micro-structure periphery sealed to be laser machined, the micro- energy-oriented-ridge of recast is formed；Finally, which is placed on corresponding cover plate in ultrasonic sealing equipment and carries out ultrasonic sealing.Present invention process simple and flexible, sealing quality is high, and the micro-fluidic chip after sealing can be applied to drug screening and the disease fields such as detection immediately.
Description
Technical field
The invention belongs to thermoplastic polymer micro-fluidic chip ultrasounds to seal technical field, and in particular to one kind being based on laser
The ultrasonic sealing method of the micro- energy-oriented-ridge of recast.
Background technology
Micro-fluidic chip based on microflow control technique just receives more after early 1990s are suggested
Carry out more concerns.Micro-fluidic chip can concentrate the mixing in biochemical analysis, and it is flat that the operations such as dilution and separation are integrated into number
On the chip of square centimetre of size.High, sample and examination with small, integrated and high degree of automation, analysis efficiency and precision
The advantages that agent consumption is few.Obtained in the fields such as detection immediately in water pollution monitoring, drug screening, cell control sorting and disease
Extensive use is arrived.Thermoplastic, polymeric materials are easy to be molded since its is at low cost, it is easy to accomplish the advantages such as mass production,
The main material of manufacture micro-fluidic chip is become gradually.
Thermoplastic polymer micro-fluidic chip is generally made of at least double-layer structure, therefore the sealing of chip is that it was manufactured
An indispensable step in journey.Present most common thermoplastic polymer micro-fluidic chip sealing method is heat sealing and sticker envelope
It closes.Wherein, heat sealing required temperature is high, the biological reagent inactivation be easy to causeing in chip.And heat sealing efficiency is low, when sealing
Between more than 20 minutes.Moreover, due to apply higher temperature and pressure during heat sealing, so chip microchannel
Easily by compressive deformation.Sticker sealing can operate at room temperature, but glued membrane used is easy and the biological reagent in chip
Generate bio-compatible sex chromosome mosaicism.And glued membrane is easy obstruction chip microchannel.
Ultrasound sealing in contrast has some superiority.Ultrasonic energy concentrates on being referred to as leading energy when ultrasound seals first
In the microprotrusion of muscle.With a certain distance from having from microchannel due to energy-oriented-ridge, the heat generated will not be to the biology in microchannel
Reagent has an impact.Secondly as ultrasound sealing in do not need other intermediate mediums, therefore not will produce bio-compatibility and in
Between dielectrically inhibited microchannel the problem of.Finally, ultrasound sealing is efficient, and the envelope to micro-fluidic chip can be completed in one minute
It closes.And ultrasonic sealing strength is high, can reach the strength of materials of thermoplastic polymer itself, this is for needing high-pressure work ring
The micro-fluidic chip in border is significant.
However, there are still some problems for ultrasonic sealing technology at this stage.Wherein most distinct issues are ultrasound sealing essences
Degree is not high.The reason of causing this problem is that under ultrasonic energy effect melt-flow can occur for energy-oriented-ridge when ultrasound is sealed,
The energy-oriented-ridge of melting easily flows into microchannel and causes to block, or on sealing interface leaves a void that reagent is caused to leak.
Most of solution is to manufacture and design special molten bath structure by energy-oriented-ridge now, to make the energy-oriented-ridge of melting flow into
Into molten bath structure.But under minute yardstick, energy-oriented-ridge and molten bath manufacture and design complicated, and need according to different micro-fluidic
Chip structure is customized.A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast proposed by the present invention can solve
This problem is of great significance for the sealing of micro-fluidic chip high-precision ultrasound.
Technical solution
It is an object of the invention to solve energy-oriented-ridge and molten bath structure design manufacture difficulty under minute yardstick, energy-oriented-ridge stream is melted
Move problem not easy to control.
Technical scheme of the present invention：
A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast, steps are as follows：
(1) manufacture and design micro-fluidic chip according to demand, which is made of cover plate 1 and substrate 4；
(2) substrate 4 is positioned with laser head, first, thermoplastic polymer tablet is fixed on laser processing platform
As alignment surface, area is more than the area of micro-fluidic chip to be sealed；Then, micro-fluidic chip step (1) designed
Substrate structure is imported into laser-processing system, a laser scanning is carried out according to micro-fluidic chip substrate structure, by the miniflow
It controls on chip substrate structure replication to alignment surface；Finally, by the micro-fluidic chip substrate structure obtained in step (1) be aligned
Replicated obtained figure is aligned in plane, until the two is completely superposed；Using laser head coordinate at this time as base
Quasi coordinates, and keep the coordinate and micro-fluidic chip substrate position constant in operating procedure afterwards；
(3) the micro- energy-oriented-ridge layout of recast is carried out, laser power, laser scanning speed and laser scanning number, edge are adjusted
The periphery of mixing pit 7 on substrate 4, comb-tooth-type delayer 8 and reaction tank 10 carries out laser scanning manufacturing, forms that recast is micro- to lead energy
Muscle 5 and molten bath 11；Microchannel corner without the micro- energy-oriented-ridge 5 of recast, as white space 6, the length of white space 6 is
1-200μm；The height of the micro- energy-oriented-ridge of recast 5 is 10-100 μm；Spacing 9 of the micro- energy-oriented-ridge of recast 5 from microchannel outer wall be
10-2000μm；
(4) substrate 4 obtained in above-mentioned steps and cover plate 1 are placed in ultrasound sealing fixture and carry out alignment and fixation, applied
After certain ultrasound sealing parameter, micro-fluidic chip is sealed from ultrasound in fixture and is taken out, completes ultrasound envelope proposed by the present invention
Conjunction process；Wherein, ultrasonic sealing pressure is 0.1-1MPa, and the ultrasonic vibration time is 0.01-1s, and ultrasonic amplitude is 10-100 μm.
Material used in micro-fluidic chip be polymethyl methacrylate, polyethylene terephthalate, polytetrafluoroethylene (PTFE),
Makrolon, polystyrene, polyethylene, polypropylene, polyvinyl chloride, nylon, polyurethane or styrene methyl methacrylate
Copolymer.
Laser on thermoplastic polymer when being scanned, and due to the effect of thermal stress, understands some material and is extruded,
Microprotrusion structure is formed in the slot wedge that laser ablation goes out.The microprotrusion structure is referred to as that recast is micro- to lead in the present invention
It can muscle.The present invention carries out ultrasonic sealing using the recast structure, and the recast of melting can flow into the groove laser machined out.
The groove of laser processing plays the role of molten bath.In this way by primary laser processing, energy-oriented-ridge and molten bath will generate simultaneously.
Eliminate complicated Design and manufacturing process.
Beneficial effects of the present invention：Ultrasound sealing method proposed by the present invention can be formed through simple laser scanning manufacturing
Micro- energy-oriented-ridge and molten bath structure do not need complicated energy-oriented-ridge and molten bath Design and manufacturing process.Recast proposed by the present invention is micro-
Energy-oriented-ridge, height can be adjusted by laser power, laser scanning speed and laser scanning number.It is proposed by the present invention
The layout designs of the micro- energy-oriented-ridge of recast can be completed by mapping software, more convenient flexible.Finally, proposed by the present invention
A kind of method sealing precision based on laser micro- recast ultrasound sealing is high, and sealing strength is high, disclosure satisfy that micro-fluidic chip into
The needs of row biochemistry detection.
Description of the drawings
Fig. 1 is the microfluidic chip structure figure sealed using this method.
Fig. 2 is sectional views of the Fig. 1 at A-A.
In figure：1 cover plate；2 liquid storage tanks；3 waste liquid pools；4 substrates；The micro- energy-oriented-ridge of 5 recasts；
6 white space around the corner without the micro- energy-oriented-ridge of recast；7 mixing pits；8 comb-tooth-type delayers；
The micro- energy-oriented-ridge of 9 recasts and microchannel outer wall spacing；10 reaction tanks；The molten bath that 11 laser ablations go out.
Specific implementation mode
Below in conjunction with attached drawing and technical solution, the specific implementation mode that further illustrates the present invention.
A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast proposed by the present invention is applied to shown in FIG. 1 micro-
In fluidic chip.The micro-fluidic chip is made of cover plate 1 and substrate 4.There are liquid storage tank 2 and waste liquid pool 3 on cover plate.In substrate 4
On have the molten bath 11 that mixing pit 7, comb-tooth-type delayer 8, reaction tank 10, the micro- energy-oriented-ridge of recast (5) and laser ablation go out.Specifically
Steps are as follows for sealing operation：
(1) pressure sintering is utilized, mixing pit 7,8 (application number of comb-tooth-type delayer are processed on substrate 4：
) and reaction tank 10 CN201610566746.8；Using milling method, liquid storage tank 2 and waste liquid pool 3 are processed on cover plate 1；
(2) substrate 4 is positioned with laser head, first, thermoplastic polymer tablet is fixed on laser processing platform
As alignment surface, area is more than the area of micro-fluidic chip to be sealed；Then, micro-fluidic chip step (1) designed
Substrate structure is imported into laser-processing system, a laser scanning is carried out according to micro-fluidic chip substrate structure, by the miniflow
It controls on chip substrate structure replication to alignment surface；Finally, by the micro-fluidic chip substrate structure obtained in step (1) be aligned
Replicated obtained figure is aligned in plane, until the two is completely superposed；Using laser head coordinate at this time as base
Quasi coordinates, and keep the coordinate and micro-fluidic chip substrate position constant in operating procedure afterwards；
(3) the micro- energy-oriented-ridge layout designs of recast are carried out, set laser power to 13W, laser scanning speed is set as
6mm/s, laser scanning number are set as 3 times, along substrate 4 mixing pit 7, comb-tooth-type delayer 8 and reaction tank 10 periphery into
Row laser scanning manufacturing forms the micro- energy-oriented-ridge 5 of recast and molten bath 11；The height of the micro- energy-oriented-ridge of recast 5 is 51 μm；Recast
Spacing 9 of the micro- energy-oriented-ridge 5 from microchannel outer wall is 200 μm；Microchannel corner without the micro- energy-oriented-ridge 5 of recast, it is as empty
The length of white region 6, white space 6 is 20 μm.
(4) substrate 4 obtained in above-mentioned steps and cover plate 1 are placed in ultrasound sealing fixture and carry out alignment and fixation, applied
After adding the ultrasonic sealing pressure of 0.3MPa, the ultrasonic sealing time of 0.07s and 40 μm of ultrasonic amplitude, by micro-fluidic chip from super
It is taken out in sound sealing fixture, completes ultrasonic sealing process proposed by the present invention.
Claims (8)
1. a kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast, which is characterized in that steps are as follows：
(1) manufacture and design micro-fluidic chip according to demand, which is made of cover plate (1) and substrate (4)；
(2) substrate (4) is positioned with laser head, first, thermoplastic polymer tablet work is fixed on laser processing platform
For alignment surface, area is more than the area of micro-fluidic chip to be sealed；Then, micro-fluidic chip base step (1) designed
Chip architecture is imported into laser-processing system, and a laser scanning is carried out according to micro-fluidic chip substrate structure, this is micro-fluidic
On chip substrate structure replication to alignment surface；Finally, the micro-fluidic chip substrate structure obtained in step (1) and being aligned is put down
Replicated obtained figure is aligned on face, until the two is completely superposed；Using laser head coordinate at this time as benchmark
Coordinate, and keep the coordinate and micro-fluidic chip substrate position constant in operating procedure afterwards；
(3) the micro- energy-oriented-ridge layout of recast is carried out, laser power, laser scanning speed and laser scanning number are adjusted, along substrate
(4) periphery of mixing pit (7), comb-tooth-type delayer (8) and reaction tank (10) carries out laser scanning manufacturing on, goes out in laser ablation
Slot wedge form microprotrusion structure, which is referred to as the micro- energy-oriented-ridge of recast (5), the groove that laser ablation goes out
Referred to as molten bath (11)；Microchannel corner without the micro- energy-oriented-ridge of recast (5), as white space (6)；
(4) substrate (4) obtained in step (3) and cover plate (1) are placed in ultrasound sealing fixture and carry out alignment and fixation, apply one
After fixed ultrasound sealing parameter, micro-fluidic chip is sealed from ultrasound in fixture and is taken out, ultrasonic sealing process is completed；Wherein, surpass
Sound sealing pressure is 0.1-1MPa, and the ultrasonic vibration time is 0.01-1s, and ultrasonic amplitude is 10-100 μm.
2. ultrasound sealing method according to claim 1, which is characterized in that the length of the white space (6) is 1-
200μm。
3. ultrasound sealing method according to claim 1 or 2, which is characterized in that the micro- energy-oriented-ridge of the recast (5)
Height is 10-100 μm.
4. it is according to claim 1 or 2 ultrasound sealing method, which is characterized in that the micro- energy-oriented-ridge of the recast (5) from
The spacing (9) of microchannel outer wall is 10-2000 μm.
5. ultrasound sealing method according to claim 3, which is characterized in that the micro- energy-oriented-ridge of the recast (5) is from micro-
The spacing (9) of channel outer wall is 10-2000 μm.
6. according to the ultrasonic sealing method described in claim 1,2 or 5, which is characterized in that the material used in micro-fluidic chip is
Polymethyl methacrylate, polyethylene terephthalate, polytetrafluoroethylene (PTFE), makrolon, polystyrene, polyethylene, poly- third
Alkene, polyvinyl chloride, nylon, polyurethane or styrene methyl methacrylate copolymer.
7. ultrasound sealing method according to claim 3, which is characterized in that the material used in micro-fluidic chip is poly- methyl
Methyl acrylate, polyethylene terephthalate, polytetrafluoroethylene (PTFE), makrolon, polystyrene, polyethylene, polypropylene, polychlorostyrene
Ethylene, nylon, polyurethane or styrene methyl methacrylate copolymer.
8. ultrasound sealing method according to claim 4, which is characterized in that the material used in micro-fluidic chip is poly- methyl
Methyl acrylate, polyethylene terephthalate, polytetrafluoroethylene (PTFE), makrolon, polystyrene, polyethylene, polypropylene, polychlorostyrene
Ethylene, nylon, polyurethane or styrene methyl methacrylate copolymer.
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| CN201611138289.9A CN106622410B (en) | 2016-12-12 | 2016-12-12 | A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast |
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| CN201611138289.9A CN106622410B (en) | 2016-12-12 | 2016-12-12 | A kind of ultrasonic sealing method based on the micro- energy-oriented-ridge of laser recast |
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| CN111958117A (en) * | 2020-08-26 | 2020-11-20 | 上海新潮雷特汽车科技有限公司 | Rib making method for shaft |
| CN113058671A (en) * | 2021-04-20 | 2021-07-02 | 中国人民解放军军事科学院军事医学研究院 | Manufacturing method of energy guiding rib for bonding of micro-fluidic chip |
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| JP2004167607A (en) * | 2002-11-15 | 2004-06-17 | Tama Tlo Kk | Microfluid element and its manufacturing method |
| CN101100107A (en) * | 2007-05-25 | 2008-01-09 | 江苏大学 | Laser plastic micro-welding method |
| CN102756474A (en) * | 2012-07-27 | 2012-10-31 | 苏州大学 | Bonding device for polymer microfluidic chip |
| CN105109034A (en) * | 2015-09-25 | 2015-12-02 | 大连理工大学 | Joint structure used for precise ultrasonic welding of POCT chip products |
| CN105833924A (en) * | 2016-03-16 | 2016-08-10 | 北京同方生物芯片技术有限公司 | Ultrasonic-bonded micro-fluidic chip and preparation method thereof |
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