CN106620405A - Traditional Chinese medicinal preparation taking tripterygium glycosides as medicinal usher and preparation method and application thereof - Google Patents
Traditional Chinese medicinal preparation taking tripterygium glycosides as medicinal usher and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicinal preparation taking tripterygium glycosides as a medicinal usher and a preparation method and an application thereof. The traditional Chinese medicinal preparation is prepared from medicinal usher liquid prepared from 300 to 400 parts of tripterygium glycosides and 100 to 200 parts of pericarpium citri reticulatae, and notopterygium, angelica pubescens, caulis pipers futokadsurae, gentiana macrophylla, ramulus cinnamomi, Chinese angelica, rhizoma chuanxiong, frankincense, radix aucklandiae, liquorice root, radix saposhnikoviae, radix angelicae dahuricae, monkshood, prepared radix aconiti, herba asari, rhizoma sinomeni cauliset, rhizoma dioscoreae colletii, coix seed, radix astragali seu hedysari, fried radix paeoniae alba, herba taxilli, achyranthes bidentate, centipede, garter snake and earthworm. The traditional Chinese medicinal preparation taking the tripterygium glycosides as the medicinal usher has the advantages of high efficiency, low adverse reaction rate, weak toxicity and the like. The traditional Chinese medicinal preparation taking the tripterygium glycosides as the medicinal usher can be applied to preparation of a medicament for treating rheumatoid arthritis, particularly rheumatoid arthritis of wind-cold-dampness arthralgia determined according to traditional Chinese medicine differentiation.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicine preparation, in particular to a kind of thunder godvine the Chinese medicine preparation of efficacy-enhancing ingredient is made
And its preparation method and application.
Background technology
Rheumatoid arthritis is a kind of chronic, multisystem, inflammatory autoimmune disease to involve based on periarticular.
Its clinical manifestation is mainly symmetry, continuation arthroncus and pain, is often accompanied by morning stiffness.Pathological manifestations are slow for synovium of joint
Property inflammation, pannus are formed, and the destruction of joint and cartilage occur.The people that the whole world has 1% is perplexed by this disease, causes serious function
Obstacle, permanent disability, and the death rate is on the rise.Treating the common drug of rheumatoid arthritis in the world at present has non-steroid
Body anti-inflammatory agent, improve state of an illness antirheumatic drug, biologic product, glucocorticoid.Although above medicine clear curative effect, its is bad anti-
Should be more, such as gastrointestinal symptom, liver and kidney dysfunction and the cardiovascular and infection adverse events that may increase.Traditional Chinese medicine is controlled
Treating rheumatoid arthritis single medicinal material the most prominent ought push away thunder godvine.Thunder godvine be the root of Celastraceae plant thunder godvine, leaf and
Flower, property of medicine bitter, acrid, cold are very toxic, and primary efficacy is wines used as antirheumatic, promoting blood circulation and removing obstruction in channels, swelling and pain relieving.
Thunder godvine and its related preparations clinically extensively should as the active drug for the treatment of rheumatoid arthritis
With.But thunder godvine has certain toxicity, and effectively treatment amount is with minimal toxic dose relatively.Occur not in the range of therapeutic dose
Good reaction is more, there is clearly infringement to systems such as digestion, uropoiesis, reproduction, angiocarpy, marrow and blood.In recent years for
The processing that thunder godvine is respectively directed in the document report for the treatment of rheumatoid arthritis, mainly with regard to thunder godvine and its preparation subtracts
The aspects such as malicious technique, preparation formulation improvement, herbal mixture compatibility, joint Western medicine and acupuncture cooperation are launched, but fundamentally do not solve
The certainly clinical toxic and side effect with Tripterygium Wilfordii Hook rheumatoid arthritis.
In view of this, it is special to propose the present invention.
The content of the invention
The first object of the present invention is to provide the Chinese medicine preparation that a kind of thunder godvine makees efficacy-enhancing ingredient, is existed with solving existing thunder godvine
The treatment big problem of rheumatoid arthritis toxic and side effect, described thunder godvine makees the Chinese medicine preparation of efficacy-enhancing ingredient, with it is efficient it is high,
The advantages of bad reaction rate is low, toxicity is weak.
The second object of the present invention is the preparation method for providing the Chinese medicine preparation that a kind of described thunder godvine makees efficacy-enhancing ingredient, should
The extract of method dried orange peel and thunder godvine dissolves Chinese traditional compound medicine, is subsequently adding drinking white spirit, makes vina, with side
Just, simple, easily operated the advantages of, it is adapted to batch production and extensively application.
The third object of the present invention is that a kind of described thunder godvine of offer makees the Chinese medicine preparation of efficacy-enhancing ingredient in preparation treatment class
Rheumathritis, especially Chinese medical discrimination are the application in the medicine of wind cold wetness type rheumatoid arthritis
In order to realize the above-mentioned purpose of the present invention, spy employs the following technical solutions:
A kind of thunder godvine makees the Chinese medicine preparation of efficacy-enhancing ingredient, in parts by weight, including by 300~400 portions of thunder godvines and 100
Efficacy-enhancing ingredient liquid and Chinese traditional compound medicine made by~200 parts of dried orange peels;
The Chinese traditional compound medicine, including following component in parts by weight:
30~50 parts of notopterygium root, 90~110 parts of levisticum, 50~70 parts of caulis piperis futokadsurae, 40~60 parts of bark of ash, 70~90 parts of cassia twig,
50~70 parts of Radix Angelicae Sinensis, 40~60 parts of Ligusticum wallichii, 10~30 parts of frankincense, 10~30 parts of the banksia rose, 50~70 parts of Radix Glycyrrhizae, windproof 50~70
Part, 50~70 parts of the root of Dahurain angelica, 50~70 parts of RADIX ACONITI LATERALIS PREPARATA, 50~70 parts of aconiti preparata,radix, 20~40 parts of the root of Chinese wild ginger, 50~70 parts of , Bi Collettiis of the root of fangji
40~60 parts, 90~110 parts of coix seed, 90~110 parts of the Radix Astragali, 50~70 parts of stir-baked RADIX PAEONIAE ALBA, 20~40 parts of parasitic loranthus, the root of bidentate achyranthes 20~
40 parts, 10~15 parts of centipede, 10~30 parts of 3~6 parts of zaocys dhumnade and earthworm.
Thunder godvine, is called herba fibraureae recisae, Huang Lateng, cabbageworm medicine, red medicine, water thick grass.Bitter, pungent, cool in nature, big poison.Return liver, kidney
Jing.With dispelling wind and eliminating dampness, remove obstruction in channels to relieve pain, swelling and pain relieving, removing toxic substances desinsection the effect of.But, thunder godvine is very toxic, produces can patient
The symptoms such as raw abdominal discomfort, nausea or diarrhoea, therefore should with caution during use to it.
Dried orange peel, it is bitter, pungent, it is warm in nature.Return lung, the spleen channel.It is eliminating dampness and eliminating phlegm with regulating qi-flowing for strengthening spleen.It is same with thunder godvine to boil, Ke Yiyou
Effect reduces the toxicity of thunder godvine, and by dried orange peel and the advance water extraction of thunder godvine, the active ingredient contributed in thunder godvine is separated out, together
When reduce its toxicity.
Notopterygium root, taste is pungent, hardship are warm in nature.Enter bladder, kidney channel.There is relieving exterior syndrome and dispelling cold, expelling wind and eliminating dampness, pain relieving.
Levisticum, taste is pungent, hardship, slightly warm in nature.Return kidney, bladder warp.There is dispelling wind and eliminating dampness, numbness pain relieving is dissipated.
Caulis piperis futokadsurae, taste is pungent, hardship, slightly warm in nature.Return liver warp.There is wines used as antirheumatic, degrading the channel, stopping numbness pain.
Bark of ash, taste is pungent, hardship are mild-natured.Return stomach, liver, gallbladder channel.With the work for having wines used as antirheumatic, clearing away damp-heat, stopping numbness pain, reducing the asthenic fever
With.
Radix Angelicae Sinensis, it is sweet, pungent, it is warm in nature.Return liver, the heart, the spleen channel.There is replenishing and activating blood, relax bowel.
Ligusticum wallichii, taste is pungent is warm in nature.Enter liver, gallbladder channel.Have functions that promote breath and open depression, dispel pathogenic wind and remove dampness, promoting blood circulation and stopping pain.
Frankincense, taste is pungent, hardship are warm in nature.The thoughts of returning home, liver, the spleen channel.There is blood-activating and qi-promoting pain relieving, detumescence and promoting granulation.
The banksia rose, taste is pungent is bitter;It is warm in nature.Returns spleen, large intestine, tri-jiao channel.There is promoting the circulation of qi, pain relieving, invigorating the spleen, help digestion.
It is windproof, taste is pungent, sweet, slightly warm in nature.Return bladder, lung, spleen, Liver Channel.Work(with the wet pain relieving that expels pathogenic wind from the body surface, wins, only convulsion
Effect.
The root of Dahurain angelica, taste is pungent is warm in nature.Return lung, spleen, stomach.With relieving exterior syndrome and dispelling cold, wind-expelling pain-stopping, clearing the nasal passage, eliminating dampness stops band, disappear
Swollen apocenosis, effect of dispelling wind and arresting itching.
RADIX ACONITI LATERALIS PREPARATA, great Xin, big heat.The thoughts of returning home, spleen, kidney channel, have functions that recuperating depleted yang, mends fire supporing yang, by wind-cold damp pathogen.
Aconiti preparata,radix, taste is pungent, hardship, property heat.The thoughts of returning home, liver,kidney,spleen Jing.There is dispelling wind and eliminating dampness, antalgic.
The root of Chinese wild ginger, taste is pungent is warm in nature.The thoughts of returning home, lung, kidney channel, with expelling wind and clearing away cold, wind-expelling pain-stopping, sensible, Wenfei of drink work(
Effect.
Cassia twig, the dry twig of canella Chinese cassia tree.It is taste is pungent, sweet, it is warm in nature.Return lung, the heart, bladder warp.With inducing sweat and dispelling exogenous evils,
Eliminating cold to stop pain, activating YANG and prormoting functioning of QI.
Radix Glycyrrhizae, it is sweet, it is mild-natured.The thoughts of returning home, lung, spleen, stomach.It is clearing heat and detoxicating with invigorating the spleen and benefiting qi, effect, in addition with
Coordinating the drug actions of a prescription, makes the effect of each herbal medicine efficacy balance.In addition, its clearing heat and detoxicating effect can effectively alleviate the poison of thunder godvine
Property.
The root of fangji, taste is pungent, hardship are cold in nature.Return bladder, lung channel.There is wind-expelling pain-stopping, inducing diuresis for removing edema.
Coix seed, have another name called the seed of Job's tears.It is sweet, light, it is cool in nature.Returns spleen, stomach, lung channel.Favourable water penetration is wet, invigorating the spleen to arrest diarrhea, except numbness,
Apocenosis, the effect of detoxicating and resolving a mass.For treating oedema, difficult urination, splenasthenic diarrhea, arthritis with fixed pain caused by dampness contraction, lung carbuncle and acute appendicitis.
Bi Collettii, it is bitter, mild-natured.Return kidney, stomach.Have functions that dampness removing removes turbid, wind expelling stagnation removing.
The Radix Astragali, it is warm in nature, it is sweet.Return lung, spleen, liver and kidney channel.There is invigorating qi for strengthening superficies, pus draining and toxin expelling, diuresis, myogenic.
Stir-baked RADIX PAEONIAE ALBA, nature and flavor are bitter, acid, are slightly cold.Return liver, the spleen channel.There is smooth liver and relieve pain, nourishing blood for regulating menstruation, astringing YIN to stop sweating.
Parasitic loranthus, it is bitter, sweet, it is mild-natured.Return liver and kidney channel.With wines used as antirheumatic, filling liver kidney, strengthening the bones and muscles effect.
The root of bidentate achyranthes, nature and flavor:Bitter, sweet, acid, puts down.Return through:Return liver and kidney channel.With dispelling stasis of blood and stimulating the menstrual flow, filling liver kidney, strengthening the bones and muscles, diuresis
Effect that is treating stranguria, ensurining proper downward flow of the blood.
Centipede, pungent, temperature.Return liver warp.With ceases wind antispastic, remove obstruction in channels to relieve pain, effect of dispersing pathogen accumulation.
Zaocys dhumnade, it is sweet, it is mild-natured.Enter lung, spleen, Liver Channel.With wines used as antirheumatic, degrading the channel, only convulsion.
Earthworm, with diuresis, ease pain, relieving asthma, being depressured, the effect such as antipyretic, anticonvulsion, can be with clearing and activating the channels and collaterals, promoting blood circulationization
The stasis of blood, the effect of prophylactic treatment cardiovascular and cerebrovascular disease.
Chinese medicine preparation provided herein, using thunder godvine as efficacy-enhancing ingredient, in order to weaken the toxicity of thunder godvine, using old
Skin carries out water extraction and obtains Aqueous extracts with thunder godvine, to reduce toxicity.It is rheumatismal using traditional treatment in Compound Chinese Herbs for Fatigue-Resistance of Animal
Chinese medicine, such as notopterygium root, levisticum, caulis piperis futokadsurae, bark of ash, centipede and ophidia, then it is aided with hot medicine, dispelling cold and removing dampness, in set part
It is evident in efficacy to wind cold wetness type rheumatoid arthritis in number.In addition, collocation spleen benefiting and stimulating the appetite Chinese medicine, return through stomach, liver, kidney,
Lung, in many-sided nourishing the stomach, heat extraction, diuresis, so as to reduce thunder godvine mechanism in toxicity, reach complete treatment rheumatismal
Effect.Wherein, notopterygium root, bark of ash, the root of Dahurain angelica, RADIX ACONITI LATERALIS PREPARATA and cassia twig is in the range of collocation provided herein, with good temperature
Sun, effect of cold dispelling.
Preferably, described thunder godvine makees the Chinese medicine preparation of efficacy-enhancing ingredient, in parts by weight, including 320~380 parts of Thunder Gods
Rattan and efficacy-enhancing ingredient liquid and Chinese traditional compound medicine made by 150~200 parts of dried orange peels;
The Chinese traditional compound medicine, including following component in parts by weight:
35~45 parts of notopterygium root, 95~105 parts of levisticum, 55~65 parts of caulis piperis futokadsurae, 45~55 parts of bark of ash, 75~85 parts of cassia twig,
55~65 parts of Radix Angelicae Sinensis, 45~55 parts of Ligusticum wallichii, 15~25 parts of frankincense, 15~25 parts of the banksia rose, 55~65 parts of Radix Glycyrrhizae, windproof 55~65
Part, 55~65 parts of the root of Dahurain angelica, 55~65 parts of RADIX ACONITI LATERALIS PREPARATA, 55~65 parts of aconiti preparata,radix, 25~35 parts of the root of Chinese wild ginger, 55~65 parts of , Bi Collettiis of the root of fangji
45~55 parts, 95~105 parts of coix seed, 95~105 parts of the Radix Astragali, 55~65 parts of stir-baked RADIX PAEONIAE ALBA, 25~35 parts of parasitic loranthus, the root of bidentate achyranthes 25~
35 parts, 12~14 parts of centipede, 15~25 parts of 2~5 parts of zaocys dhumnade and earthworm.
The component of Chinese medicine preparation can be carried out preferably.
Preferably, the thunder godvine is the root of the under ground portion belt leather of thunder godvine.
The root of under ground portion, band yellow root skin person, its skin contains various chemical compositions, and active force is strong, is that treatment rheumatoid is closed
The scorching active drug of section.
Preferably, the preparation method of the efficacy-enhancing ingredient liquid is comprised the following steps:
Soak in the water that thunder godvine and dried orange peel are added 10~12 times of thunder godvine quality, boiling is first heated to high heat, then
It is changed to be cooked by slow fire, obtains the efficacy-enhancing ingredient liquid equivalent to 2~4 times of the thunder godvine quality.
Preferably, the temperature of the intense fire is more than 180 DEG C, and the temperature of the slow fire is 80~120 DEG C.
Preferably, the time of the immersion is 2~3 hours, and the time being cooked by slow fire is 2~3 hours.
Thunder godvine and dried orange peel are extracted using the method for water extraction, as efficacy-enhancing ingredient liquid, can effectively weaken thunder godvine
Toxicity.
Preferably, also including drinking white spirit, the drinking white spirit is (0.8~1.0) with the volume ratio of the efficacy-enhancing ingredient liquid:1.
Preferably, the number of degrees of the drinking white spirit are 40~50 degree.
Drinking white spirit is added in Chinese medicine preparation, medicinal liquor is prepared into, the active ingredient in Chinese medicine can be extracted, it is right
Active ingredient in Chinese medicine is preferably absorbed.
Thunder godvine as above makees the preparation method of the Chinese medicine preparation of efficacy-enhancing ingredient, comprises the following steps:
Chinese traditional compound medicine is added in efficacy-enhancing ingredient liquid, heating is allowed to dissolve, after cooling, add drinking white spirit, after mixing
Make the Chinese medicine tincture of efficacy-enhancing ingredient to the thunder godvine;
It is furthermore preferred that the Chinese traditional compound medicine is selected to exempt to decoct Chinese medicine.
Thunder godvine as above makees answering in medicine of the Chinese medicine preparation of efficacy-enhancing ingredient in treatment rheumatoid arthritis is prepared
With especially Chinese medical discrimination is the application in the medicine of the rheumatoid arthritis of wind-cold-dampness arthralgia.
Compared with prior art, beneficial effects of the present invention are:
(1) thunder godvine provided herein makees the Chinese medicine preparation of efficacy-enhancing ingredient, the extract prepared using dried orange peel and thunder godvine
As efficacy-enhancing ingredient, the toxicity of thunder godvine is effectively reduced, coordinate the Chinese medicine of eliminating cold to remove dampness, can be with effectively treatment rheumatism, and Chinese medicine system
Composition in agent also containing spleen benefiting and stimulating the appetite, diuresis, it is reasonably combined on number with the Chinese medicine for the treatment of rheumatism, effectively reduce thunder godvine
Toxicity and alleviate the discomfort that causes to patient's belly, play a part of Synergy and attenuation.
(2) thunder godvine provided herein makees the preparation method of the Chinese medicine preparation of efficacy-enhancing ingredient, with it is convenient, simple, be easy to
The advantages of operation, it is adapted to batch production and extensively application.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with specific embodiment, but ability
Field technique personnel will be understood that, following described embodiment is a part of embodiment of the invention, rather than the embodiment of whole,
The present invention is merely to illustrate, and is not construed as limiting the scope of the present invention.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained under the premise of creative work is not made, belongs to the model of present invention protection
Enclose.Unreceipted actual conditions person in embodiment, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or instrument
Unreceipted production firm person, being can pass through the conventional products that commercially available purchase is obtained.
Embodiment 1
The thunder godvine that the present embodiment is provided makees the Chinese medicine preparation of efficacy-enhancing ingredient, including what is prepared by 300g thunder godvines and 100g dried orange peels
Efficacy-enhancing ingredient liquid, and following Chinese medicinal component:Notopterygium root 30g, levisticum 90g, caulis piperis futokadsurae 50g, bark of ash 40g, cassia twig 70g, Radix Angelicae Sinensis 50g, Ligusticum wallichii
40g, frankincense 10g, banksia rose 10g, Radix Glycyrrhizae 50g, windproof 50g, root of Dahurain angelica 50g, RADIX ACONITI LATERALIS PREPARATA 50g, aconiti preparata,radix 50g, root of Chinese wild ginger 20g, the root of fangji
50g , Bi Collettiis 40g, coix seed 90g, Radix Astragali 90g, stir-baked RADIX PAEONIAE ALBA 50g, parasitic loranthus 20g, root of bidentate achyranthes 20g, centipede 10g, zaocys dhumnade 3g and
Earthworm 10g.
Its preparation method, comprises the following steps:
(1) soak 2 hours in the water that, thunder godvine and dried orange peel are added 3000ml, boiling is first heated to high heat, then be changed to
It is cooked by slow fire 2 hours, obtains 800ml efficacy-enhancing ingredient liquid;
(2), exempt from above-mentioned in the efficacy-enhancing ingredient liquid that pan-fried Chinese traditional compound medicine is added in step (1), heating melts Chinese medicine, drops
40 degree of drinking white spirit 800ml are added to room temperature, the Chinese medicine tincture that the thunder godvine makees efficacy-enhancing ingredient is obtained after mixing.
Embodiment 2
The thunder godvine that the present embodiment is provided makees the Chinese medicine preparation of efficacy-enhancing ingredient, including what is prepared by 400g thunder godvines and 200g dried orange peels
Efficacy-enhancing ingredient liquid, and following Chinese medicinal component:Notopterygium root 50g, levisticum 110g, caulis piperis futokadsurae 70g, bark of ash 60g, cassia twig 90g, Radix Angelicae Sinensis 70g, Ligusticum wallichii
60g, frankincense 30g, banksia rose 30g, Radix Glycyrrhizae 70g, windproof 70g, root of Dahurain angelica 70g, RADIX ACONITI LATERALIS PREPARATA 70g, aconiti preparata,radix 70g, root of Chinese wild ginger 40g, the root of fangji
70g , Bi Collettiis 60g, coix seed 110g, Radix Astragali 110g, stir-baked RADIX PAEONIAE ALBA 70g, parasitic loranthus 40g, root of bidentate achyranthes 40g, centipede 15g, zaocys dhumnade 6g
With earthworm 30g.
Its preparation method, comprises the following steps:
(1) soak 2 hours in the water that, thunder godvine and dried orange peel are added 3500ml, boiling is first heated to high heat, then be changed to
It is cooked by slow fire 2 hours, obtains 1200ml efficacy-enhancing ingredient liquid;
(2), exempt from above-mentioned in the efficacy-enhancing ingredient liquid that pan-fried Chinese traditional compound medicine is added in step (1), heating melts Chinese medicine, drops
50 degree of drinking white spirit 1000ml are added to room temperature, the Chinese medicine tincture that the thunder godvine makees efficacy-enhancing ingredient is obtained after mixing.
Embodiment 3
The thunder godvine that the present embodiment is provided makees the Chinese medicine preparation of efficacy-enhancing ingredient, including what is prepared by 320g thunder godvines and 150g dried orange peels
Efficacy-enhancing ingredient liquid, and following Chinese medicinal component:Notopterygium root 35g, levisticum 95g, caulis piperis futokadsurae 55g, bark of ash 45g, cassia twig 75g, Radix Angelicae Sinensis 55g, Ligusticum wallichii
45g, frankincense 15g, banksia rose 15g, Radix Glycyrrhizae 55g, windproof 55g, root of Dahurain angelica 55g, RADIX ACONITI LATERALIS PREPARATA 55g, aconiti preparata,radix 55g, root of Chinese wild ginger 25g, the root of fangji
55g , Bi Collettiis 45g, coix seed 95g, Radix Astragali 95g, stir-baked RADIX PAEONIAE ALBA 55g, parasitic loranthus 25g, root of bidentate achyranthes 25g, centipede 12g, zaocys dhumnade 2g and
Earthworm 15g.
Its preparation method, comprises the following steps:
(1) soak 3 hours in the water that, thunder godvine and dried orange peel are added 3000ml, boiling is first heated to high heat, then be changed to
It is cooked by slow fire 3 hours, obtains 1000ml efficacy-enhancing ingredient liquid;
(2), exempt from above-mentioned in the efficacy-enhancing ingredient liquid that pan-fried Chinese traditional compound medicine is added in step (1), heating melts Chinese medicine, drops
50 degree of drinking white spirit 800ml are added to room temperature, the Chinese medicine tincture that the thunder godvine makees efficacy-enhancing ingredient is obtained after mixing.
Embodiment 4
The thunder godvine that the present embodiment is provided makees the Chinese medicine preparation of efficacy-enhancing ingredient, including what is prepared by 380g thunder godvines and 200g dried orange peels
Efficacy-enhancing ingredient liquid, and following Chinese medicinal component:Notopterygium root 45g, levisticum 105g, caulis piperis futokadsurae 65g, bark of ash 55g, cassia twig 85g, Radix Angelicae Sinensis 65g, Ligusticum wallichii
55g, frankincense 25g, banksia rose 25g, Radix Glycyrrhizae 65g, windproof 65g, root of Dahurain angelica 65g, RADIX ACONITI LATERALIS PREPARATA 65g, aconiti preparata,radix 65g, root of Chinese wild ginger 35g, the root of fangji
65g , Bi Collettiis 55g, coix seed 105g, Radix Astragali 105g, stir-baked RADIX PAEONIAE ALBA 65g, parasitic loranthus 35g, root of bidentate achyranthes 35g, centipede 14g, zaocys dhumnade 5g
With earthworm 25g.
Its preparation method, comprises the following steps:
(1) soak 3 hours in the water that, thunder godvine and dried orange peel are added 4000ml, boiling is first heated to high heat, then be changed to
It is cooked by slow fire 2 hours, obtains 1100ml efficacy-enhancing ingredient liquid;
(2), exempt from above-mentioned in the efficacy-enhancing ingredient liquid that pan-fried Chinese traditional compound medicine is added in step (1), heating melts Chinese medicine, drops
50 degree of drinking white spirit 1000ml are added to room temperature, the Chinese medicine tincture that the thunder godvine makees efficacy-enhancing ingredient is obtained after mixing.
Embodiment 5
The thunder godvine that the present embodiment is provided makees the Chinese medicine preparation of efficacy-enhancing ingredient, including what is prepared by 350g thunder godvines and 150g dried orange peels
Efficacy-enhancing ingredient liquid, and following Chinese medicinal component:Notopterygium root 40g, levisticum 100g, caulis piperis futokadsurae 60g, bark of ash 50g, cassia twig 80g, Radix Angelicae Sinensis 60g, Ligusticum wallichii
50g, frankincense 20g, banksia rose 20g, Radix Glycyrrhizae 60g, windproof 60g, root of Dahurain angelica 60g, RADIX ACONITI LATERALIS PREPARATA 60g, aconiti preparata,radix 60g, root of Chinese wild ginger 30g, the root of fangji
60g , Bi Collettiis 50g, coix seed 100g, Radix Astragali 100g, stir-baked RADIX PAEONIAE ALBA 60g, parasitic loranthus 30g, root of bidentate achyranthes 30g, centipede 13g, zaocys dhumnade 3g
With earthworm 20g.
Its preparation method, comprises the following steps:
(1) soak 2~3 hours in the water that, thunder godvine and dried orange peel are added 4000ml, boiling is first heated to high heat, then
It is changed to be cooked by slow fire 2~3 hours, obtains 1000ml efficacy-enhancing ingredient liquid;
(2), exempt from above-mentioned in the efficacy-enhancing ingredient liquid that pan-fried Chinese traditional compound medicine is added in step (1), heating melts Chinese medicine, drops
50 degree of drinking white spirit 1000ml are added to room temperature, the Chinese medicine tincture that the thunder godvine makees efficacy-enhancing ingredient is obtained after mixing.
Comparative example 1
Comparative example 1 is the efficacy-enhancing ingredient liquid provided in the step of embodiment 5 (1).
Comparative example 2
Comparative example 2 is substantially the same manner as Example 5, simply when efficacy-enhancing ingredient liquid is decocted, only adds 350g thunder godvines and is added without
Dried orange peel.
Comparative example 3
Comparative example 3 is substantially the same manner as Example 5, simply in Chinese traditional compound medicine, removes the Chinese medicine of Appetizing spleen-tonifying:Radix Angelicae Sinensis,
Radix Glycyrrhizae, coix seed, frankincense, the banksia rose, the root of Dahurain angelica, the Radix Astragali and stir-baked RADIX PAEONIAE ALBA.
Comparative example 4
Comparative example 4 is substantially the same manner as Example 5, simply in Chinese traditional compound medicine, removes the Chinese medicine of expelling wind and clearing away cold:Notopterygium root,
Bark of ash, the root of Dahurain angelica, RADIX ACONITI LATERALIS PREPARATA and cassia twig.
The thunder godvine of experimental example 1 makees impact of the Chinese medicine preparation of efficacy-enhancing ingredient to II Collagen Type VI induced rat rheumatoid arthritis
(1) experimental subjects
Wistar big white mouse, body weight (180 ± 20) g, Hubei Prov. Academy of Medical Sciences's Experimental Animal Center, quality certification number SCXK
(Hubei Province) 200320005.
(2) Experimental agents
The thunder godvine that the application institute embodiment 1-5 is provided makees the Chinese medicine preparation of efficacy-enhancing ingredient, and comparative example 1-4 is provided
Chinese medicine.
(3) experimental technique
The foundation of rat CIA models, is dissolved in 0.1mol/L acetic acid (content 2mg/mL), by C II before experiment by acid C II
Dissolving adds to (1mg/mL) in cold incomplete Freund's adjuvant, causes in the left back sufficient plantar intracutaneous injection 0.25mL of every rat scorching.
Wistar rats 110 are taken, male and female half and half are randomly divided into 11 groups, i.e. Normal group, model control group, implemented
Example 1-5 administration groups and comparative example 1-4 administration group (dosage 1mg/kg).Normal group injection normal saline is made to cause scorching thing
Matter.Cause scorching same day administration, continuous 28d.Respectively at causing before inflammation, cause to be amplified with capillary within the 14th, 16,18,20,24,28 days after inflammation
Mensuration surveys right metapedes volume, so that be worth based on scorching front volume, obtains swelling gain percentage [(after administration before value-administration
Value) the front value of/administration] × 100.
Rat causes put to death for the 28th day after inflammation, and aseptic routine prepares SPL suspension, and trypan blue dye liquor carries out living cells
Calculate and be more than 95, adjustment cell concentration to final concentration of 1 × 106/ mL, with containing the RPMI-1640 nutrient solutions that volume fraction is 10
Cultured cells, by the μ L of every hole 100 96 porocyte culture plates are inoculated in, and give 100 μ LConA (5 μ g/mL) or LPS (5 μ g/mL) points
Not Ci Ji T, bone-marrow-derived lymphocyte, separately set and do not give the self-blank control wells that ConA and LPS stimulate, each sample sets 3 per experimental point
Multiple holes.37℃、5CO2Culture 44h;20 μ LMIT (5mg/mL) are added per hole, continues to cultivate to 48h, terminate culture, culture is abandoned in suction
Liquid, adds 200 μ LDMSO terminating reactions, vibration to shake up per hole.ELIASA detects (570/630nm) A values.
At rat after death, swelling foot pawl is won at 0.5cm above right ankle joint rapidly.It is longitudinally slit, it is put into 5mL cold
In physiological saline, in 4 DEG C of soaked overnights, centrifuging and taking supernatant, filtration sterilization, -20 DEG C, NO, PGE to be measured are preserved2Content.
The tissue of lower foot plantar position is taken fully, is fixed in time with 10% neutral buffered formalin, FFPE, cut into slices, paster
(slide is soaked in advance with poly-D-lysine, 60 DEG C of heating 60min is dragged for after piece, so that section is closely attached), routinely dewax to
Water, 0.1mol/LPBS (pH=7.4) immersion 5min.Microwave method antigen, is then added dropwise confining liquid, gets rid of surplus liquid.It is added dropwise
The NF- к β/p65 sheep IgG monoclonal antibody and MMP-9 mouse IgG monoclonal antibodies of 50 dilutions, negative control replaces one with PBS
It is anti-, in 4 DEG C of overnight incubations.0.1mol/LPBS is washed 3 times, every time 5 minutes.
As a result judge:There is faint yellow fine particulate with endochylema and be colored as positive cell, optical microphotograph Microscopic observation, per group
500 cells of random counter, calculate positive expression rate, while observing staining power, colour by staining power yellowish for weakly positive
(+), pale brown for positive (++), sepia is strong positive (+++), and non-coloring is negative (-).
Experimental result represents that significance test between group is carried out with t-test, and data analysis is soft using spss10.0 with x ± s
Part.
Medicine is as shown in table 1 to the inhibitory action experimental result of the induced rat foot swellings of C II.To CIA rat spleen T, B lymphs
The impact experimental result of cell proliferative response is as shown in table 2.To CIA rat spleen cells factors IL-1β、TNF-α, IL-6 contents
Affect as shown in table 3.To NO, PGE in CIA rat articular immersion liquid2The impact of content is as shown in table 4.CIA rat groups pawl is organized
The impact of middle NF- к β/p65 protein positive expressions rate and MMP-9 protein positive expression rates is as shown in table 5.
Inhibitory action (x ± s, n=10) of the table 1 to the induced rat foot swellings of C II
Compare with model group:*P<0.05,**P<0.01;Compare with normal group△△P<0.01 (table 2,3,4 is same)
Test result indicate that, model group rats cause inflammation 20d or so swelling in causing 14d or so after inflammation secondary foot swelling occur
Degree peaking.Compare with model group, the thunder godvine that the application is supplied makees the work that the Chinese medicine preparation of efficacy-enhancing ingredient shows foot swelling inhibition
With showing that the Chinese medicine preparation can significantly inhibit the secondary affection of CIA rats.Also, the effect of embodiment group is better than comparative example group.
Table 2 is to CIA rat spleen T, the impact (x ± s, n=10) of B lymphocyte proliferation activity
Test result indicate that, compared with normal group, the spleen T of model group rats ConA, LPS induction, B lymphocyte proliferation are lived
Significantly raised (the P of property<0.01);Compare the thunder godvine that the application supplied with model group and make the Chinese medicine preparation of efficacy-enhancing ingredient and suppress CIA rats
Proliferation activity (the P of the T lymphocytes of ConA inductions<0.01) the B lymphocyte proliferation activity (P, and to LPS inductions<0.01).
Also, the effect of embodiment group is better than comparative example group.
Table 3 is to CIA rat spleen cells factors IL-1β、TNF-α, IL-6 contents impact
Model group rats splenocyte factor IL-1β、TNF-α, IL-6 contents rising (P notable than normal group<0.01), originally
The supplied thunder godvine of application makees the Chinese medicine preparation group and comparative example group indices of efficacy-enhancing ingredient and is remarkably decreased (P compared with model group<
0.05,P<0.01) Chinese medicine preparation, is pointed out to significantly inhibit CIA rat spleen cells factors IL-1β、TNF-α, IL-6 level.
Also, the effect of embodiment group is better than comparative example group.
Table 4 is to NO, PGE in CIA rat articular immersion liquid2The impact of content
NO, PGE in the immersion liquid of model group rats joint2Content substantially increases (P compared with normal group<0.01), the application institute
For thunder godvine make NO, PGE in the Chinese medicine preparation group joint immersion liquid of efficacy-enhancing ingredient2Content is substantially less than model group (P<0.01).Show
The Chinese medicine preparation significantly inhibits NO, PGE in CIA rat articular immersion liquid2Level.Also, the effect of embodiment group is better than contrast
Example group.
Table 5 is to NF- к β/p65 protein positive expressions rate and MMP-9 protein positive expression rates in CIA rat groups pawl tissue
Affect
Test result indicate that, the thunder godvine that the application is supplied make the Chinese medicine preparation group of efficacy-enhancing ingredient can substantially reduce NF- к β/
P65 albumen and MMP-9 protein positive expression rate (P<0.01).Show that the Chinese medicine preparation significantly reduces the positive expression of two kinds of albumen
Rate.Also, the effect of embodiment group is better than comparative example group.
To sum up, thunder godvine provided herein makees the Chinese medicine preparation of efficacy-enhancing ingredient, can have significant curative effect to arthritis.By right
The contrast of ratio and experimental example group, comparative example 1 is the simple thunder godvine extract without any Chinese medicine, and toxicity is big, effect
Difference.And comparative example 2 may certify that, the addition of dried orange peel can effectively suppress the toxicity of thunder godvine, improve drug effect.Comparative example 3 and right
Ratio 4 is demonstrated respectively, and the Chinese medicine of the spleen benefiting and stimulating the appetite for being added and warm cold dispelling has a facilitation to drug effect, and add
The Chinese medicine of spleen benefiting and stimulating the appetite can effectively suppress the toxicity of thunder godvine.
The thunder godvine of experimental example 2 makees the Chinese medicine preparation bad reaction test of efficacy-enhancing ingredient
(1) animal used as test
SPF level KM mouse, male and female half and half, 18~22g of body weight is of the right age, health.Purchased from Shandong University's Experimental Animal Center,
Animal credit number:SCXK (Shandong) 20090001.Animal used as test mixed feed (mouse), production licence number:SCXK (Shandong)
20090014。
(2) experimental technique
Mouse 90 is taken, water 12h is can't help in male and female half and half, fasting, with the thunder that concentration is provided embodiment 1-5 by 1mg/kg
Public rattan makees the Chinese medicine that the Chinese medicine preparation and comparative example 1-4 of efficacy-enhancing ingredient provided carries out continuous gavage 3 times, per eight hours once.Every time
Will 15min after observed and recorded medicine, the toxic reaction and death condition of 30min, 1h, 2h, 4h, 8h.
Experimental result is as shown in table 6.
The bad reaction of table 6 records result
Test result indicate that, using after the medicine medicine feed that comparative example 1 is provided, mouse death rate is high, when occurring first
Time is several after medicine between 24-72h, and comparative example 2-4 decreases than the death rate of comparative example 1, but be the absence of it is appropriate in
Medicine proportioning, have impact on the toxicity slow releasing function to thunder godvine Aqueous extracts, cause mouse bad reaction rate and the death rate high.
The thunder godvine of experimental example 3 makees the Chinese medicine preparation of efficacy-enhancing ingredient to Germ Cells of Male Mice toxicity research
(1) animal used as test and contamination
Healthy Male Kunming strain mice 66,30~35g of body weight is randomly divided into 11 groups, and the embodiment of the present application 1-5 is carried
For thunder godvine make the Chinese medicine preparation (dosage 1mg/kg) that the Chinese medicine preparation and comparative example of efficacy-enhancing ingredient are provided.Negative control group,
The physiological saline of 1mg/kg is gavaged, is gavaged daily, continue three weeks, adjust dose by body weight weekly.24h puts to death after drug withdrawal.
Positive controls, intraperitoneal injection of cyclophosphamide 100mg/kg injects 2d, and 3d puts to death.
(2) experimental technique
(1) Teratogenic effects
Cervical dislocation puts to death mouse, and hind leg femur, calf serum cleaning ossis, push jack are taken rapidly.Methyl alcohol is fixed
15min, Giensa dye 10~15min, air-dry.Every mouse counts and contain in 1000 polychromatic erythrocytes micronucleus under oil mirror
Polychromatic erythrocyte, calculates micronucleus permillage.
(2) sperm shape abnormality test
300 sperms of every animal of observation, record distortion incidence.
Experimental result is as shown in table 7.
The Teratogenic effects of table 7 and sperm shape abnormality test result
Test result indicate that, micronuclear rates can react Newborn to cellular damage degree and the stability of chromosome.Adopt
The Chinese medicine preparation provided with the embodiment of the present application and comparative example, there is different degrees of mutagenic effect to cell.Using comparative example 1
After the medicine medicine feed for being provided, mouse cell micronuclear rates is high, and comparative example 2-4 decreases than the micronuclear rates of comparative example 1, but
Lack appropriate Chinese medicine proportioning, have impact on the toxicity slow releasing function to thunder godvine Aqueous extracts, cause mouse cell micronuclear rates and essence
Sub- abnormal rate is high.Wherein, comparative example 1 is the simple thunder godvine extract without any Chinese medicine, and toxicity is big, and effect is poor.And
Comparative example 2 may certify that the addition of dried orange peel can effectively suppress the toxicity of thunder godvine, improve drug effect.Comparative example 3 and comparative example 4
Demonstrate respectively, the Chinese medicine of the spleen benefiting and stimulating the appetite for being added and warm cold dispelling has facilitation, and the invigorating the spleen added to drug effect
The Chinese medicine of appetizing can effectively suppress the toxicity of thunder godvine.
Although illustrate and describing the present invention with specific embodiment, but will be appreciated that various embodiments above is only used
To illustrate technical scheme, rather than a limitation;It will be understood by those within the art that:Without departing substantially from this
In the case of bright spirit and scope, the technical scheme described in foregoing embodiments can be modified, or to wherein
Some or all of technical characteristic carries out equivalent;And these modifications or replacement, do not make the essence of appropriate technical solution
Depart from the scope of various embodiments of the present invention technical scheme;It is, therefore, intended that including belonging to the present invention in the following claims
In the range of all these substitutions and modifications.
Claims (10)
1. a kind of thunder godvine makees the Chinese medicine preparation of efficacy-enhancing ingredient, it is characterised in that in parts by weight, including by 300~400 parts of thunders
Public rattan and efficacy-enhancing ingredient liquid and Chinese traditional compound medicine made by 100~200 parts of dried orange peels;
The Chinese traditional compound medicine, including following component in parts by weight:
30~50 parts of notopterygium root, 90~110 parts of levisticum, 50~70 parts of caulis piperis futokadsurae, 40~60 parts of bark of ash, 70~90 parts of cassia twig, Radix Angelicae Sinensis
50~70 parts, 40~60 parts of Ligusticum wallichii, 10~30 parts of frankincense, 10~30 parts of the banksia rose, 50~70 parts of Radix Glycyrrhizae, windproof 50~70 parts, in vain
50~70 parts of the root of Dahurian angelica, 50~70 parts of RADIX ACONITI LATERALIS PREPARATA, 50~70 parts of aconiti preparata,radix, 20~40 parts of the root of Chinese wild ginger, 50~70 parts of , Bi Collettiis 40~60 of the root of fangji
Part, 90~110 parts of coix seed, 90~110 parts of the Radix Astragali, 50~70 parts of stir-baked RADIX PAEONIAE ALBA, 20~40 parts of parasitic loranthus, 20~40 parts of the root of bidentate achyranthes,
10~30 parts of 10~15 parts of centipede, 3~6 parts of zaocys dhumnade and earthworm.
2. thunder godvine according to claim 1 makees the Chinese medicine preparation of efficacy-enhancing ingredient, it is characterised in that in parts by weight, bag
Include 320~380 portions of thunder godvines and efficacy-enhancing ingredient liquid and Chinese traditional compound medicine made by 150~200 parts of dried orange peels;
The Chinese traditional compound medicine, including following component in parts by weight:
35~45 parts of notopterygium root, 95~105 parts of levisticum, 55~65 parts of caulis piperis futokadsurae, 45~55 parts of bark of ash, 75~85 parts of cassia twig, Radix Angelicae Sinensis
55~65 parts, 45~55 parts of Ligusticum wallichii, 15~25 parts of frankincense, 15~25 parts of the banksia rose, 55~65 parts of Radix Glycyrrhizae, windproof 55~65 parts, in vain
55~65 parts of the root of Dahurian angelica, 55~65 parts of RADIX ACONITI LATERALIS PREPARATA, 55~65 parts of aconiti preparata,radix, 25~35 parts of the root of Chinese wild ginger, 55~65 parts of , Bi Collettiis 45~55 of the root of fangji
Part, 95~105 parts of coix seed, 95~105 parts of the Radix Astragali, 55~65 parts of stir-baked RADIX PAEONIAE ALBA, 25~35 parts of parasitic loranthus, 25~35 parts of the root of bidentate achyranthes,
15~25 parts of 12~14 parts of centipede, 2~5 parts of zaocys dhumnade and earthworm.
3. thunder godvine according to claim 1 and 2 makees the Chinese medicine preparation of efficacy-enhancing ingredient, it is characterised in that the thunder godvine is thunder
The root of the under ground portion belt leather of public rattan.
4. thunder godvine according to claim 1 and 2 makees the Chinese medicine preparation of efficacy-enhancing ingredient, it is characterised in that the system of the efficacy-enhancing ingredient liquid
Preparation Method is comprised the following steps:
Soak in the water that thunder godvine and dried orange peel are added 10~12 times of thunder godvine quality, boiling is first heated to high heat, then be changed to
It is cooked by slow fire, obtains 2~4 times of efficacy-enhancing ingredient liquid of thunder godvine quality.
5. thunder godvine according to claim 4 makees the Chinese medicine preparation of efficacy-enhancing ingredient, it is characterised in that
The temperature of the intense fire is more than 180 DEG C, and the temperature of the slow fire is 80~120 DEG C.
6. thunder godvine according to claim 4 makees the Chinese medicine preparation of efficacy-enhancing ingredient, it is characterised in that the time of the immersion is 2
~3 hours, the time being cooked by slow fire was 2~3 hours.
7. thunder godvine according to claim 1 and 2 makees the Chinese medicine preparation of efficacy-enhancing ingredient, it is characterised in that also including drinking white spirit,
The drinking white spirit is (0.8~1.0) with the volume ratio of the efficacy-enhancing ingredient liquid:1.
8. thunder godvine according to claim 7 makees the Chinese medicine preparation of efficacy-enhancing ingredient, it is characterised in that the number of degrees of the drinking white spirit
For 40~50 degree.
9. the thunder godvine according to any one of claim 1-8 makees the preparation method of the Chinese medicine preparation of efficacy-enhancing ingredient, it is characterised in that
Comprise the following steps:
Chinese traditional compound medicine is added in efficacy-enhancing ingredient liquid, heating is allowed to dissolve, after cooling, add drinking white spirit, be somebody's turn to do after mixing
Thunder godvine makees the Chinese medicine tincture of efficacy-enhancing ingredient;
Preferably, the Chinese traditional compound medicine is selected and exempts to decoct Chinese medicine.
10. the thunder godvine described in any one of claim 1-8 makees the Chinese medicine preparation of efficacy-enhancing ingredient in treatment rheumatoid arthritis is prepared
Medicine in application, preferred rheumatoid arthritis Chinese medical discrimination be wind-cold-dampness arthralgia.
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Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104587090A (en) * | 2015-02-04 | 2015-05-06 | 王爱英 | Medicine for treating rheumatoid arthritis |
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2017
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Publication number | Priority date | Publication date | Assignee | Title |
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CN104587090A (en) * | 2015-02-04 | 2015-05-06 | 王爱英 | Medicine for treating rheumatoid arthritis |
Non-Patent Citations (2)
Title |
---|
谌曦等: "《名老中医风湿病诊疗经验》", 30 September 2013, 安徽科学技术出版社 * |
高学敏: "《中药知识》", 31 August 1988, 科学普及出版社 * |
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