CN106620234A - Cough-relieving traditional Chinese medicine composition and preparation method and application thereof - Google Patents
Cough-relieving traditional Chinese medicine composition and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8968—Ophiopogon (Lilyturf)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention relates to the field of traditional Chinese medicine, in particular to a cough-relieving traditional Chinese medicine composition and a preparation method and application thereof. The cough-relieving traditional Chinese medicine composition is prepared from, by weight, 500-900 g of Folium Eriobotryae, 500-900 g of Radix Ophiopogonis, 100-200 g of Herba Taraxaci, 100-200 g of Radix Isatidis and 50-100 g of Radix Glycyrrhizae in terms of dried medicinal herbs. The cough-relieving traditional Chinese medicine composition is good in cough relieving effect, simply and easily available to components, simple in preparation technology and easy for industrialization and has high development value and social and economic benefits. The invention further discloses a cough-relieving pharmaceutical preparation. The cough-relieving pharmaceutical preparation comprises an effective dose of traditional Chinese medicine composition as an active ingredient and one or more pharmaceutically acceptable carriers.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition field, is specifically related to a kind of Chinese medicine composition for cough-relieving.
Background technology
Cough is the protectiveness respiration reflex action of the secretion that human body is removed in respiratory tract or foreign matter.Although there is its favourable
One side, but acutely chronic cough can cause hemorrhage from respiratory tract.
Traditional Chinese medicine thinks that cough is, because of the diseases caused by external factors six external factors which cause diseases, internal organs internal injury, to affect the sound disease for having phlegm caused by lung, sees《Element
Ask that the five internal organs generate a piece》.《Plain Questions interpretation of the cause, onset and process of an illness gas is preferably saved one's life collection》:" cough meaning it is sound without phlegm, lung qi wound and it is unclear;It is noiseless to cough
And have phlegm, spleen is wet dynamic and be phlegm.Cough meaning has phlegm and sound, and lid moves wet in spleen in lung qi due to wound, coughs and is to cough ".Because outer
Heresy violates lung, or internal organs internal injury, involves caused by lung.
At present the treatment of cough is sufficiently complex, and cough is most commonly in flu, and priming factorses of catching a cold are very more, use carelessly
Antibiotic etc medicine, it may be difficult to comprehensively treated.Traditional Chinese medicine is particular about wholistic therapy, fundamentally solves illness, opens
The Chinese medicine for sending out effective cough-relieving a kind of is particularly important.
Loguat leaf, the tuber of dwarf lilyturf, Radix Isatidis, dandelion, the chinese medicine material conduct of Radix Glycyrrhizae five that inventor is enriched from china natural resources
Research material,
Criticize leaf
Loguat leaf (scientific name:Eriobotrya japonica Thunb.) for rosaceous plant loquat leaf.Also known as Ba Ye, reed
Tangerine leaf(《Chinese medicine handbook》).There are removing heat from the lung to relieve cough, and stomach diuresis, the effect quenched the thirst.There are treatment lung feverish cough, hemoptysis, bleeding from five sense organs or subcutaneous tissue, stomach
Heat vomit the sound of vomiting effect.
The tuber of dwarf lilyturf
The tuber of dwarf lilyturf(Scientific name:Ophiopogon japonicus(Linn. f.) Ker-Gawl.)Liliaceae Ophiopogon is perennial
Evergreen herbage, root is thicker, and middle or proximal end often expands ovalisation or fusiform little root tuber, and stem is very short, phyllopodium life
Cheng Cong, standing grain is lobate, and bract lanceolar, tip is tapering, and seed is spherical, the month at florescence 5-8, the fruiting period 8-9 month.The tuber of dwarf lilyturf originates in China, day
Sheet, Vietnam, India are also distributed.There is cultivation on the ground such as southern china.The dark and damp place in hillside, the sylvan life being born in below 2000 meters of height above sea level
Or by small stream.The little root tuber of the tuber of dwarf lilyturf is Chinese medicine, the effect for have quench one's thirst of promoting the production of body fluid, moistening the lung and relieve the cough.
Radix Isatidis
Radix Isatidis, Chinese medicine name commonly uses another name:Indigo-blue, indigo root, manyflower glorybower root, are a kind of Chinese medicines.Hardship, trembles with fear.With heat-clearing solution
The effect of poison, cool blood profit pharynx.It is usually used in poison during warm epidemic disease, pharyngalgia of generating heat, febrile virulent maculae, mumps, scarlet fever, major part pestilence is red
Poison, carbuncle swells.
Dandelion
Dandelion(Classification system:Taraxacum mongolicumHand.-Mazz.)Composite family, Dandelion perennial herb
Plant.Root is coniform, surface sepia, shrinkage, and limb edge has wave-formed mouth or pinniform drastic crack sometimes, and base portion is gradually narrow into petiole, leaf
Handle and master pulse are often with reddish violet, and scape top aubergine is close by the long pubescence of spider's thread shape white;Capitulum, involucre mitriform, achene
Crineous, long pappus white, 4~October of flowering fruit bearing stage.Dandelion another name dandelion, Po Poding, Hua Hualang etc..Composite family is perennial
Herbaceous plant.Capitulum, there is the bobbles that white pappus are formed on seed, rack after the flowers are in blossom to breeding new life where new
Life.Nature and flavor are sweet, slight bitter, tremble with fear.Return liver, stomach, the effects such as have diuresis, laxative, removing jaundice subcutaneous ulcer, cholagogic
Radix Glycyrrhizae
Radix Glycyrrhizae(Scientific name:Glycyrrhiza uralensis Fisch)Another name:State is old, Herba Hedyotis cantonensis, Glycyrrhiza Uralensis, sweet root.Beans
Section, Glycyrrhiza perennial herb, root is sturdy with root-like stock, is a kind of help Chinese herbal medicine.To a kind of good medicine of human body, medicinal portion
Position is root and rhizome, and Medicinal Materials Characters root is long 25~100 centimetres in cylinder, 0.6~3.5 centimetre of diameter.Crust is elastic to differ,
Surface rufous or taupe brown.In cylinder, there is bud scar on surface to rhizome, and there is marrow at section middle part.Gas is micro-, and taste is sweet and special.Function
Cure mainly clearing heat and detoxicating, expelling phlegm and arresting coughing, gastral cavity abdomen etc..
At home, substantial amounts of research is carried out to the Chinese medicine for cough-relieving, patent CN103656579B discloses one kind to be included
Exocarpium Citri Rubrum, dried orange peel, the tuber of pinellia, Poria cocos, balloonflower root, almond, perillaseed, aster, tussilago, PERICARPIUM TRICHOSANTHIS, Bulbus Fritillariae Cirrhosae, the dried rhizome of rehmannia, the tuber of dwarf lilyturf, ginger etc.
A kind of Chinese medicine composition prepared by tens kinds of medicinal materials, component is especially complex, and component is more in actual production process, production process
It is difficult to control to, product effect is difficult to ensure that between criticizing;Patent CN103655847B discloses a kind of Chinese medicine for cough-relieving, including
Radix bupleuri, dried orange peel, rhizoma atractylodis, gingko benevolence, Radix Glycyrrhizae, walnut kernel, rock sugar, almond etc., main crushing after mixing makes electuary, step
Although simple, it is also well known that preferably after certain effective component extracting, curative effect can be more preferable for general raw medicinal herbs.
The content of the invention
Problem to be solved by this invention is to provide a kind of Chinese medicine composition for treating cough-relieving, and it is by batch leaf
The alcohol extract composition of enzymatic hydrolyzed extract and Radix Isatidis, Radix Glycyrrhizae, dandelion with the tuber of dwarf lilyturf, with crude drug weight note, loguat leaf:500g-
900g;The tuber of dwarf lilyturf:500g-900g;Dandelion:100g-200g ;Radix Isatidis:100g-200g;Radix Glycyrrhizae:50g-100g is constituted.
The preferred alcohol extract by the enzymatic hydrolyzed extract and Radix Isatidis criticized leaf and the tuber of dwarf lilyturf, Radix Glycyrrhizae, dandelion is constituted, with life
Medicine weight note, loguat leaf:600g-800g ;The tuber of dwarf lilyturf:600g-800g;Dandelion:140g-160g;Radix Isatidis:140g-160g;
Radix Glycyrrhizae:70g-90g is constituted.
The optimum alcohol extract by the enzymatic hydrolyzed extract and Radix Isatidis, Radix Glycyrrhizae, dandelion criticized leaf and the tuber of dwarf lilyturf is constituted, with life
Medicine weight note, loguat leaf:700g;The tuber of dwarf lilyturf:700g;Dandelion:150g;Radix Isatidis:150g;Radix Glycyrrhizae:80g is constituted.
Prepare to implement above-mentioned Chinese medicine, comprise the steps:
(1)Loguat leaf and the tuber of dwarf lilyturf, add 2-6 times and measure water, soak 1 hour or more, add equivalent to batch leaf and tuber of dwarf lilyturf gross weight
The 0.5%-5% cellulose complex enzymes of amount, digest 2-6 hours, and hydrolysis temperature is controlled between 40-70 DEG C, after enzymolysis, is warming up to
90-100 DEG C, kept for 5-10 minutes, kill enzyme, filtered, concentration adds ethanol solution, regulation solution concentration to 60% or more, alcohol
It is heavy 6 hours or more, filter, obtain solution 1;
(2)Radix Isatidis, Radix Glycyrrhizae and dandelion add 2-4 times to measure ethanol solution, and refluxing extraction 2-6 hour is extracted twice, filtered,
Obtain solution 2;
(3)Solution 1 and solution 2 are mixed, concentration is obtained final product.
In order to prepare Chinese medicine composition, preparation method further includes that step is as follows,
(1)Loguat leaf and the tuber of dwarf lilyturf, add 3-5 times and measure water, soak 1 hour or more, add equivalent to batch leaf and tuber of dwarf lilyturf gross weight
The 1%-3% cellulose complex enzymes of amount, digest 3-5 hours, and hydrolysis temperature is controlled between 50-60 DEG C, after enzymolysis, is warming up to 90-
100 DEG C, kept for 5-10 minutes, kill enzyme, filtered, concentration adds ethanol solution, regulation solution concentration to 60% or more, alcohol precipitation 6
Hour or more, filter, obtain solution 1;
(2)Radix Isatidis, Radix Glycyrrhizae and dandelion add 2-4 times to measure ethanol solution, and refluxing extraction 3-5 hour is extracted twice, filtered,
Obtain solution 2;
(3)Solution 1 and solution 2 are mixed, concentration is obtained final product.
The optimal preparation method of the present invention is as follows:
(1)Loguat leaf and the tuber of dwarf lilyturf, 4 times of amount water are added, soaked 1 hour or more, added equivalent to batch leaf and tuber of dwarf lilyturf gross weight
2% cellulose complex enzyme, digest 4 hours, hydrolysis temperature is controlled at 55 DEG C, after enzymolysis, is warming up to 90-100 DEG C, keeps 5-10
Minute, enzyme is killed, to filter, concentration adds ethanol solution, adjusts solution ethanol concentration to 60%, and alcohol precipitation 6 hours is filtered, and obtains solution
1;
(2)Radix Isatidis, Radix Glycyrrhizae and dandelion add 3 times of amount ethanol solutions, refluxing extraction 4 hours to extract twice, filter, and obtain molten
Liquid 2;
(3)Solution 1 and solution 2 are mixed, concentration is obtained final product.
According to the Chinese medicine composition that above-mentioned step is prepared, oral liquid formulations, its step can be further prepared into
Appropriate water is added including by resulting composition, is filtered, filling, sterilizing obtains final product oral administration solution.The Chinese medicine of certain present invention
Composition can also make other preparations, as long as including above-mentioned composition, all within interest field.
The present invention obtains enzymolysis liquid by the way that loguat leaf, the tuber of dwarf lilyturf are digested;Radix Isatidis, dandelion, three kinds of medicinal materials of Radix Glycyrrhizae
Directly extracted with ethanol, obtain alcohol extract;Above-mentioned enzymolysis liquid and alcohol extract are mixed again, are condensed into the solution of certain density,
Described Chinese medicine composition is obtained final product, oral liquid or other oral formulations can be further prepared into.
The bright extraction by way of enzymolysis of this law was criticized the leaf tuber of dwarf lilyturf, can be good at extracting the active ingredient in medicinal material,
Increase the curative effect of medicine.After mixing with the alcohol extract of Radix Isatidis, dandelion, Radix Glycyrrhizae, cough suppressing effect tool increases significantly,
Simultaneously by the mouse of the investigation long-term taking present composition, any toxic and side effect is had no.
The effect of each experimental group and control group is compared by the cough-relieving test of mouse, rat resolving sputum experiment.
Experiment packet such as table 1 below:
1. mouse is drawn and coughs experiment (concentrated ammonia liquor spray-on process)
Kunming mouse 90(Jin Feng animals used as test Co., Ltd of Jinan City, credit number:SCXK (Shandongs)2014 0006), body
Weight 18-22g, male and female half and half are placed in special glass bell jar, carry out 25 ﹪ concentrated ammonia liquors using ultrasonic atomizatio sprayer quantitative
Spraying, takes out immediately after 10 seconds, records mouse and starts to the incubation period for cough occur from spraying is received, and coughing in 2 minutes
Cough number of times.2 minutes not cough persons give up after spraying.Qualified mouse be grouped at random according to above-mentioned group technology after 2 days (blank group in
Draw to cough and select packet at random before experiment, do not carry out concentrated ammonia liquor spraying).Per group is pressed respectively each matched doses continuous gavage 5 in table 1
My god, blank group gives normal saline with negative control group.1 hour after last dose, 25 ﹪ concentrated ammonia liquors are received respectively and is determined
Amount spraying, takes out immediately after 10 seconds.Record mouse starts to the cough in the incubation period and 2 minutes for cough occur from spraying is received
Number of times, is had no its incubation period of cough person and was remembered with 120 seconds in 2 minutes.
Antitussive effect of each group extract of table 2 to mouse(X ± s, n=10)
Remarks:Cough-relieving rate(%)=administration group Respiratory frequency the time/negative control group Respiratory frequency time * 100%, positive control has 3
Dead mouse.
Sulfur dioxide stimulus method
Kunming mouse(Jin Feng animals used as test Co., Ltd of Jinan City, credit number:SCXK (Shandongs)2014 0006), body weight
18-22g, male and female half and half are randomly divided into 9 groups, 10 per group, such as table 1.Each administration group according to dosage gastric infusion (20g/kg) is right
Normal saline is given according to group, once a day, continuous 3d.1h after last dose, mouse is put into the wide-mouth bottle of 250mL, note
Enter sulfur dioxide gas 4mL, observe the incubation period of mouse cough, and calculate cough-relieving rate.
The each group cough suppressing effect result statistical form of table 3(X ± s, n=10)
Remarks:Cough-relieving rate is consistent with the computing formula of table 1.
(capillary glass-tube method) is tested in resolving sputum
Take SD rats 70(Jin Feng animals used as test Co., Ltd of Jinan City, credit number:SCXK (Shandongs)2014 0006), male and female
Half and half, it is randomly divided into 7 groups, 10 per group, such as table 4.Water 12h, urethane 1g/kg lumbar injections fiber crops are can't help in fasting before each group experiment
It is liquor-saturated, face upward position and fix, neck middle part skin is cut off, escaping pipe is separated, hit exactly between two cartilaginous rings with sharply in thyroid gland cartilage lower edge
Injection needle prick an aperture, be inserted into internal diameter 018cm capillary glass tubies one, make capillary just contact tracheae bottom table
Face, the sputum so as to drawing tracheae rear portion draws sputum length as the resolving sputum effect for evaluating medicine using capillary.Record administration
After the normal secretions amount of front 1h, each administration group according to dosage gastric infusion (10mL/kg), control group gives equal-volume distilled water, remembers
The sputum secretory volume of record administration 1h each group rats.The results are shown in Table 4.
Table 4:Result of each experimental group to rat expectoration(X ± s, n=10)
Experimental result shows that the product obtained by the present invention has significant effect, in rat resolving sputum in terms of mouse cough-relieving
Aspect also has significant effect, therefore product of the present invention is used for cough-relieving aspect and has good curative effect, while the invention belongs to a kind of
Tcm product, the general toxic and side effect of Chinese medicine is little, and using safety, and preparation technology of the present invention is simple, and medicinal material is easy to get, and is producing greatly
Use is more facilitated, and can bring huge economic benefit to society.
Experiment is investigated in side effect
Kunming mouse 40(Jin Feng animals used as test Co., Ltd of Jinan City, credit number:SCXK (Shandongs)2014 0006), it is female
Male half and half, administration group is divided at random(Embodiment 2 is obtained)And control group(Purified water).Administration group gives 10 mL, control group to give pure
Change the mL of water 10, two groups are 2 times in 1d, every 12h gavages 1 time.Continuous Observation 14d after administration, per 3d to body weight and food-intake
Recorded.As a result from table 5, body weight becomes before and after two groups of mouse administrations.
Mouse Weight and the change (X ± s, g) per daily inleting appetite before and after the administration of table 5
Jing paired t-tests, equal no difference of science of statistics (P > 0.05).Observe the general indicator of animal simultaneously, such as outward appearance, behavior, right
Reaction, secretion, excreta of stimulation etc., without significantly abnormal.Dead mouse is had no during test.After off-test, mouse
Conventinal breeding 1 week, respectively sacrificed by decapitation, mouse is dissected, and each internal organs color is normal, organizes no abnormality seen, and abdominal cavity has no swollen thing.
Infer mouse dose equivalent to human body according to above-mentioned experiment(Body weight 50kg per capita)1250 times, continuous gavage has no mouse in 14 days
Death, and mice viscera is normal, therefore the toxic and side effect of the present invention is very little.
Specific embodiment
With reference to embodiments further illustrating the present invention, advantages of the present invention and feature will be with description and more
It is clear.But these embodiments are only exemplary, do not constitute any restriction to the scope of the present invention.Those skilled in the art should
It should be appreciated that, the details of technical solution of the present invention and form can be repaiied without departing from the spirit and scope of the invention
Change or replace, but these modifications and replacement are each fallen within protection scope of the present invention.
Criticize and leaf, the tuber of dwarf lilyturf, Radix Isatidis, Radix Glycyrrhizae, dandelion are purchased from Hunan Province Lian Qiao Chinese medicine specialized markets
Cellulose complex enzyme:The taste bio tech ltd of Hebei hundred, vigor:The units of 10-40 ten thousand
Comparative example 1
Loguat leaf 1.4kg, tuber of dwarf lilyturf 1.4Kg are weighed, in extractor, 4 times of amount water is added, is soaked 2 hours, add 70g celluloses
Complex enzyme, digests 4 hours, and hydrolysis temperature is controlled at 55 DEG C, after enzymolysis, is warming up to 90-100 DEG C, is kept for 8 minutes, kills enzyme, mistake
Filter, concentration adds ethanol solution, adjusts solution concentration to 60%, and alcohol precipitation 6 hours is filtered, and is concentrated to give extract 200.8g.
Comparative example 2
Weighing Radix Isatidis 600g, Radix Glycyrrhizae 600g, dandelion 320g adds 3 times of amount ethanol solutions, refluxing extraction 4 hours to extract two
It is secondary, filter, filtered fluid is obtained, it is concentrated to give extract 203.1g.
Comparative example 3
Weigh and criticize the ethanol for leaf 1.4kg, tuber of dwarf lilyturf 1.4kg, Radix Isatidis 300g, Radix Glycyrrhizae 300g, dandelion 160g being added 3 times of amounts
Solution, refluxing extraction 4 hours is extracted twice, obtains filtered fluid, is concentrated to give extract 205,1g.
Comparative example 4
Weigh and criticize leaf 1.4kg, tuber of dwarf lilyturf 1.4kg, add 4 times of amount water, heating and refluxing extraction 4 hours is extracted 2 times, filtered, dense
Contracting, adds ethanol solution, makes solution ethanol concentration reach 60%, static 12 hours, filters, and obtains filtrate 1;Radix Isatidis 300g, Radix Glycyrrhizae
300g, dandelion 160g, add the ethanol solution of 3 times of amounts, stream to extract 4 hours, extract twice, obtain filtered fluid 2, merging filtrate 1
With filtrate 2, extract 201.2g is concentrated to give.
Embodiment 1
Loguat leaf 1.4kg, tuber of dwarf lilyturf 1.4kg are weighed, 11.2kg water is added, is soaked 1 hour, add 56g cellulose complex enzymes, enzymolysis
4 hours, hydrolysis temperature was controlled at 55 DEG C, after enzymolysis, was warming up to 90 DEG C, was kept for 10 minutes, killed enzyme, was filtered, and concentration adds ethanol
Solution, adjusts solution concentration to 60%, and alcohol precipitation 6 hours is filtered, and obtains solution 1;
Weighing Radix Isatidis 300g, Radix Glycyrrhizae 300g, dandelion 160g adds 2.28kg ethanol solutions, refluxing extraction 4 hours to extract two
It is secondary, filter, obtain solution 2;
Solution 1 and solution 2 are mixed, 203.3g is concentrated to give.
Embodiment 2
The solution of the gained of half embodiment 1 is taken, adds water to be diluted to 1L, filtered, filling into ampoule bottle, every 10ml(Quite
In crude drug:Loguat leaf 7g, tuber of dwarf lilyturf 7g, Radix Isatidis 1.5g, Radix Glycyrrhizae 1.5g, dandelion 0.8g), instructions of taking for twice daily,
One time one.
Embodiment 3
Loguat leaf 1.0kg, tuber of dwarf lilyturf 1.0kg are weighed, 4kg water is added, is soaked 1 hour, add 10g cellulose complex enzymes, enzymolysis 2 is little
When, hydrolysis temperature is controlled at 40 DEG C, after enzymolysis, is warming up to 90 DEG C, is kept for 10 minutes, kills enzyme, is filtered, concentration, adds ethanol molten
Liquid, adjusts solution concentration to 60%, and alcohol precipitation 6 hours is filtered, and obtains solution 1;
Weighing Radix Isatidis 200g, Radix Glycyrrhizae 200g, dandelion 100g adds 1.0kg ethanol solutions, refluxing extraction 2 hours to extract two
It is secondary, filter, obtain solution 2;
Solution 1 and solution 2 are mixed, extract 201.3g is concentrated to give.
Embodiment 4
Loguat leaf 1.8kg;The tuber of dwarf lilyturf:1.8kg adds 21.6kg water, soaks 1 hour, adds 180g cellulose complex enzymes, enzymolysis 6
Hour, hydrolysis temperature is controlled at 70 DEG C, after enzymolysis, is warming up to 90 DEG C, is kept for 5 minutes, kills enzyme, is filtered, concentration, adds ethanol molten
Liquid, adjusts solution concentration to 60%, and alcohol precipitation 6 hours is filtered, and obtains solution 1;
Dandelion:400g;Radix Isatidis:400g;Radix Glycyrrhizae:200g is constituted.4kg ethanol solutions, refluxing extraction 6 hours is added to extract
Twice, filter, obtain solution 2;
Solution 1 and solution 2 are mixed, extract 201.7g is concentrated to give.
Embodiment 5
Loguat leaf 1.6kg;The tuber of dwarf lilyturf:1.6kg adds 16kg water, soaks 1 hour, adds 96g cellulose complex enzymes, and enzymolysis 5 is little
When, hydrolysis temperature is controlled at 60 DEG C, after enzymolysis, is warming up to 90 DEG C, is kept for 5 minutes, kills enzyme, is filtered, concentration, adds ethanol molten
Liquid, adjusts solution concentration to 60%, and alcohol precipitation 6 hours is filtered, and obtains solution 1;
Dandelion:320g;Radix Isatidis:320g;Radix Glycyrrhizae:180g is constituted.3.28kg ethanol solutions, refluxing extraction 5 hours is added to carry
Take twice, filter, obtain solution 2;
Solution 1 and solution 2 are mixed, extract 209.2g is concentrated to give.
Embodiment 6
Loguat leaf 1.2kg;The tuber of dwarf lilyturf:1.2kg adds 7.2kg water, soaks 1 hour, adds 24g cellulose complex enzymes, and enzymolysis 3 is little
When, hydrolysis temperature is controlled at 50 DEG C, after enzymolysis, is warming up to 90 DEG C, is kept for 5 minutes, kills enzyme, is filtered, concentration, adds ethanol molten
Liquid, adjusts solution concentration to 60%, and alcohol precipitation 6 hours is filtered, and obtains solution 1;
Dandelion:280g;Radix Isatidis:280g;Radix Glycyrrhizae:140g is constituted.1.4kg ethanol solutions, refluxing extraction 3 hours is added to carry
Take twice, filter, obtain solution 2;
Solution 1 and solution 2 are mixed, extract 209.2g is concentrated to give.
Claims (7)
1. a kind of Chinese medicine composition for cough-relieving, it is by batch leaf and enzymatic hydrolyzed extract and Radix Isatidis, Radix Glycyrrhizae, the Pu Gong of the tuber of dwarf lilyturf
The alcohol extract composition of English, with crude drug weight note,
Loguat leaf 500g-900g tuber of dwarf lilyturf 500g-900g
Dandelion 100g-200g Radix Isatidis 100g-200g
Radix Glycyrrhizae 50g-100g.
2. the Chinese medicine composition for cough-relieving according to claim 1, wherein with crude drug weight note,
Loguat leaf 600g-800g tuber of dwarf lilyturf 600g-800g
Dandelion 140g-160g Radix Isatidis 140g-160g
Radix Glycyrrhizae 70g-90g.
3. the Chinese medicine composition for cough-relieving according to claim 1, wherein with crude drug weight note,
Loguat leaf 700g tuber of dwarf lilyturf 700g
Dandelion 150g Radix Isatidis 150g
Radix Glycyrrhizae 80g.
4. the Chinese medicine composition for cough-relieving according to any one of claim 1-3, wherein the extract Jing is with lower section
Method is obtained:
(1)Loguat leaf and the tuber of dwarf lilyturf, add 2-6 times and measure water, soak 1 hour or more, add equivalent to batch leaf and tuber of dwarf lilyturf gross weight
The 0.5%-5% cellulose complex enzymes of amount, digest 2-6 hours, and hydrolysis temperature is controlled between 40-70 DEG C, after enzymolysis, is warming up to
90-100 DEG C, kept for 5-10 minutes, kill enzyme, filtered, concentration adds ethanol solution, regulation solution concentration to 60% or more, alcohol
It is heavy 6 hours or more, filter, obtain solution 1;
(2)Radix Isatidis, Radix Glycyrrhizae and dandelion add 2-4 times to measure ethanol solution, and refluxing extraction 2-6 hour is extracted twice, filtered,
Solution 2;
(3)Solution 1 and solution 2 are mixed, concentration is obtained final product.
5. the preparation method of the Chinese medicine composition for cough-relieving according to claim 4, takes and is characterised by:Preparation process is such as
Under,
(1)Loguat leaf and the tuber of dwarf lilyturf, add 3-5 times and measure water, soak 1 hour or more, add equivalent to batch leaf and tuber of dwarf lilyturf gross weight
The 1%-3% cellulose complex enzymes of amount, digest 3-5 hours, and hydrolysis temperature is controlled between 50-60 DEG C, after enzymolysis, is warming up to 90-
100 DEG C, kept for 5-10 minutes, kill enzyme, filtered, concentration adds ethanol solution, regulation solution concentration to 60% or more, alcohol precipitation 6
Hour or more, filter, obtain solution 1;
Radix Isatidis, Radix Glycyrrhizae and dandelion add 2-4 times to measure ethanol solution, and refluxing extraction 3-5 hour is extracted twice, filtered, it is molten
Liquid 2;
(3)Solution 1 and solution 2 are mixed, concentration is obtained final product.
6. the preparation method of the Chinese medicine composition for cough-relieving according to claim 4, takes and is characterised by:Preparation process is such as
Under,
(1)Loguat leaf and the tuber of dwarf lilyturf, 4 times of amount water are added, soaked 1 hour or more, added equivalent to batch leaf and tuber of dwarf lilyturf gross weight
2% cellulose complex enzyme, digest 4 hours, hydrolysis temperature is controlled between 55 DEG C, after enzymolysis, is warming up to 90-100 DEG C, is kept
5-10 minutes, enzyme is killed, filtered, concentration adds ethanol solution, adjust solution ethanol concentration to 60%, alcohol precipitation 6 hours is filtered, and is obtained
Solution 1;
(2)Radix Isatidis, Radix Glycyrrhizae and dandelion add 3 times of amount ethanol solutions, refluxing extraction 4 hours to extract twice, filter, it is molten
Liquid 2;
(3)Solution 1 and solution 2 are mixed, concentration is obtained final product.
7. the preparation method of the Chinese medicine composition for cough-relieving according to any one of claim 4-6, it is characterised in that:Institute
Obtained concentrate adds appropriate water, filters, filling, and sterilizing obtains final product oral administration solution.
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