CN106620045A - Preparation method and application of 95% ethanol extract of clerodendrum japonicum roots - Google Patents
Preparation method and application of 95% ethanol extract of clerodendrum japonicum roots Download PDFInfo
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- CN106620045A CN106620045A CN201611254326.2A CN201611254326A CN106620045A CN 106620045 A CN106620045 A CN 106620045A CN 201611254326 A CN201611254326 A CN 201611254326A CN 106620045 A CN106620045 A CN 106620045A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
- A61K36/855—Clerodendrum, e.g. glorybower
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention discloses a preparation method and an application of a 95% ethanol extract of clerodendrum japonicum roots, and belongs to the technical field of medicine. Specifically, the preparation method comprises the following steps: crushing the clerodendrum japonicum roots, conducting reflux extraction, filtering an extracting solution, combining filtrates, conducting extraction by virtue of 95% ethanol, combining extracting liquid, conducting concentrating and conducting drying, so that the 95% ethanol extract of the clerodendrum japonicum roots is obtained. The invention also discloses the application of the 95% ethanol extract of the clerodendrum japonicum roots in preparing anti-inflammatory medicines. The 95% ethanol extract of the clerodendrum japonicum roots can be used for preparing the anti-inflammatory medicines, so that novel treatment medicines are provided for patients with inflammations; and various side effects in the application of current anti-inflammatory medicines can be reduced.
Description
Technical field
The present invention relates to pharmaceutical technology field, the She of Ju bodies is Ji the preparation method and application of the ethanol extract of Cheng forture paulownia root 95%.
Background technology
Inflammation is the most basic damage-retardation traumatic response of body, any that the factor of tissue damage can be caused all to become inflammation
Reason.Its Clinical symptoms is red, swollen, hot, pain, is that pro-inflammatory cytokine stimulates the biological tissue with vascular system to local damage
Reaction.Inflammation has three different phases：First phase is acute or inflammation early stage, and the period blood vessel is upset, blood vessel endothelium
Gap enlargement, Vascular Permeability strengthens, and leucocyte and protein etc. are exuded to tissue space, cause swollen tissue, therefore, swell
Swollen is the important indicator of inflammation early stage；Second phase is inflammation mid-term, with the characteristics of platelet adhesion reaction and leukoplania, and is exempted from
Epidemic disease reaction is closely related；3 phase is chronic inflammation or the later stage of inflammation, period endothelial cell, fibroblast and the essence
The hyperplasia such as cell, form granulation tissue hyperplasia, with the characteristics of proliferation of fibrous tissue, Granuloma formation.What is commonly used at present is anti-
Scorching medicine is mostly Western medicine, and Western medicine has certain side effect using after, and to body chronic injury is caused.
Cheng paulownia is Verenaceae smalt platymiscium Cheng paulownia Clerodendrum japonicum（Thunb.）Sweet., and
The red dragon-boat of name, Flos clerodendri japonici, taking out must red, akebi lotus, pearl Chinese parasol tree, " individual friend's quilt "（Zhuang nationality in Guangxi language）, being that Guangxi is conventional strengthens medicine, medicine
It is root with position,《Chinese book on Chinese herbal medicine》、《Conventional glossary of Herbs and Drugs among the people》Record its sweet, cool in nature, complete stool can be used as medicine, its flower can pacify
God, hemostasis, cure mainly palpitation and insomnia, hemorrhoid hemorrhage；The effects such as there is leaf wind-dispelling to dissipate the stasis of blood, removing toxicity for detumescence, cures mainly antimigraine, the bruise stasis of blood
Swollen, carbuncle sore tumefacting virus；Root, stem can clearing lung-heat heat, diuresis, cooling blood and hemostasis, be clinically used for cough with lung heat, heat gonorrhea, difficult urination, cough
Blood, hematuria, hemorrhoid hemorrhage, the treatment for the treatment of rheumatic ostealgia, with good clinical value.
Among the people strong medicinal material of the Cheng forture paulownia root as Guangxi, although have certain application Zhuang is among the people, but so far still without entering
Row is developed well, and the open report of current Dui Cheng paulownia research is less.
The content of the invention
The invention aims to solve the problems referred to above of prior art presence, the ethanol extract of Ti Gong Cheng forture paulownia root 95%
Preparation method and application.
To reach above-mentioned purpose, the technical solution used in the present invention is as follows：
The preparation method of the ethanol extract of Cheng forture paulownia root 95%, comprises the steps：
（1）Cheng forture paulownia root is crushed, 80% ethanol solution refluxing extraction of 8 times of amounts 3 times, each 2h, temperature 70 C is filtered, and merges filter
Liquid, filtrate puts Rotary Evaporators decompression recycling ethanol, and concentrate puts water-bath Back stroke ethanol solution to the greatest extent, and Gan Zao get Cheng forture paulownia root ethanol is total
Extract；
（2）Jiang Cheng forture paulownia root ethanol total extract medicinal extract, after being dissolved in water, extracted 5 times with isopyknic 95% ethanol, merged each time
Extract, after recycling design, get is Dao the ethanol extract of Cheng forture paulownia root 95%.
Application of Shang ethanol extract of Shu Cheng forture paulownia root 95% in anti-inflammatory medicaments are prepared.
Shang ethanol extract of Shu Cheng forture paulownia root 95% is in the application for preparing anti-inflammation medicine.
Application of Shang ethanol extract of Shu Cheng forture paulownia root 95% in anti-chronic inflammation medicine is prepared
Beneficial effect：The present invention extracts the ethanol extract of Cheng forture paulownia root 95% can be to acute inflammation and the swollen tissue of chronic inflammation
There is significant inhibitory action.The enforcement of the present invention can provide raw material for the exploitation for going deep into anti-inflammatory drug.Jiang the ethanol of Cheng forture paulownia root 95%
Extract is used to prepare anti-inflammatory medicaments, can provide new medicine for inflammatory disease patients, can reduce using anti-inflammatory at present
The various side effects that medicine occurs.
Specific embodiment
The present invention implementation process in, using to and NM technology is prior art.
Embodiment
（1）The preparation of test medicine
The preparation of the ethanol extract of Cheng forture paulownia root 95%, step is as follows：
The preparation of Cheng forture paulownia root ethanol total extract：Jiang Cheng forture paulownia root 5880g, crush, 80% ethanol solution refluxing extraction 3 of 8 times of amounts
Secondary, each 2h, temperature 70 C is filtered, and merging filtrate, filtrate puts Rotary Evaporators decompression recycling ethanol, and concentrate is put in water-bath
Wave most ethanol solution, Gan Zao get Cheng forture paulownia root ethanol total extract 838.7g.
The preparation of the ethanol extract of Cheng forture paulownia root 95%：By the total ethanol extract medicinal extract of Shang Shu Cheng forture paulownia roots, after being dissolved in water, use
Isopyknic 95% ethanol is extracted 5 times, and each extract is merged respectively, after recycling design, obtains 95% ethanol extract 391.5g.
Benzylpenicillin sodium for injection 0.48g is weighed respectively and streptomycin sulphate for injection 0.8g mixings are dissolved to distilled water
80ml obtains final product dual anti-test liquid, shakes up, -20 DEG C of preservations.
（2）Mouse granuloma induced by implantation of cotton pellets
Healthy Kunming mouse 96 is taken, male is weighed, and is randomly divided into 8 groups, 12 per group, respectively blank control group, positive
Control group（Dexamethasone, 0.006g/ kg）The high, medium and low dosage group of the ethanol extract of ， Cheng forture paulownia root 95%（80、40、20g/ kg）.
After Animal Anesthesia, 2 autoclaving and the 10mg cotton balls of the dual anti-test liquids of 0.1ml is added dropwise and has been implanted into mouse both sides armpit respectively
Under, apply the tincture of iodine after sewing up the incision outward anti-infective.Respectively gavage gives corresponding medicine to next day each group mouse, and blank group gives corresponding body
Long-pending distilled water.1 time/d, continuous 7d puts to death mouse after last dose 12h, peel off the cotton balls of granulation tissue, and precision weighs cotton balls
Weight in wet base, 60 DEG C are baked to constant weight, and precise weighing calculates granulation quality.
Granulation weight=the weight of the cotton balls with granulation tissue-cotton balls original weight
（3）Carrageenan causes mouse foot swelling
Kunming mice 96, weighs, and packet and dosage are same（2）, per group press administered volume 20ml/kg gastric infusion, 1 time/
D, continuous 7d.After last dose, after every mouse metapedes is marked with eminence with marker pen mark, with toes swelling capacity measurer
Measurement toes normal volume, and record.In the carrageenan 0.02ml of mouse toes hypodermic injection 1%, 0.5 after Yu Zhiyan, 1.5,
3.5th, 4.5,5.5h measurements toes swelling volume, calculates swelling rate.
Inhibiting rate %=（Cause V after inflammation2- cause scorching front V1）/ cause scorching front V1×100%
（4）The impact of paraxylene induced mice ear swelling and swelling inhibiting rate
The healthy Kunming mouse of 18～22g of body weight is selected, male, random packet, is divided into blank control group, sun by 10 per group
Property control group (the high, medium and low dosage group of the ethanol extract of dexamethasone), Cheng copper root 95%, gavage (ig) administration, daily 1 time,
Continuous 6 d.After last dose, dimethylbenzene is uniformly applied to mouse right ear two sides and causes to swell, left ear is compared.De- cervical vertebra is put to death little
Mouse, along auricle baseline two ears are cut, and round auricle is laid at the same position of two ears respectively with card punch, are weighed, with same mouse two
The difference of auricle weight represents swelling, and calculates swelling inhibiting rate.
Inhibiting rate=(model control group swelling-experimental group swelling)/model control group swelling × 100%.
（5）Acetic acid causes the experiment of mouse peritoneal capillary permeability
Mouse 60, is randomly divided into model group, Dexamethasone group（0.0045 g/kg）The ethanol extract of, Cheng copper root 95% height,
In, low dose group（40,20,10 g/kg）, 12 per group.Gastric infusion, is administered the bodies such as the ml/kg of volume 20, model group gavage
Product distilled water.It is administered daily 1 time, continuous 7 d.After the min of last dose 45, the Evans blue of each tail vein injection 0.25% is molten
The ml/10 g of liquid 0.1, while the glacial acetic acid solution of lumbar injection 0.6%, per only 0.2 ml.Cervical vertebra is taken off after 15 min and puts to death animal,
The ml of intraperitoneal injection of saline 6 rinses abdominal cavity, gently rubs the min of belly 1, cuts off abdominal cavity and collects peritoneal fluid, centrifugation（1 000
rpm/min）10 min, take supernatant mensuration absorbance at the nm of 723 spectrophotometer 590.
（6）As a result
Impacts of a to mouse granuloma induced by implantation of cotton pellets
The granuloma induced by implantation of cotton pellets weight of each dosage group mouse of the ethanol extract of Cheng forture paulownia root 95% is substantially reduced compared with model group, and difference has
Conspicuousness（P ＜ 0.001 or P ＜ 0.01）, Qi ethanol extract high dose group of Zhong Cheng forture paulownia root 95% is to mouse granuloma induced by implantation of cotton pellets weight in wet base
Inhibiting rate is most notable, up to 31.96%, the results are shown in Table 1.
Impact of the ethanol extract of 1 Cheng paulownia of table 95% to mouse granuloma induced by implantation of cotton pellets
Note：Compare with model group, * * * P ＜ 0.001, * * P ＜ 0.01.
B Carrageenans cause the impact of mouse foot swelling
The swelling of each dosage group mouse foot swelling of the ethanol extract of Cheng forture paulownia root 95% is substantially reduced compared with model group, and difference has aobvious
Work property（P ＜ 0.01 or P ＜ 0.05）, in Qi ethanol extract of Zhong Cheng forture paulownia root 95%, low dose group to mouse foot swelling inhibiting rate most
Significantly, up to 37.18%.The results are shown in Table 2.
The ethanol extract Carrageenan of 2 Cheng forture paulownia root of table 95% causes the impact of mouse swollen feet
Note：Compare with model group, * * * P ＜ 0.001, * * P ＜ 0.01, * P ＜ 0.05.
C paraxylene causes the impact of mice auricle swelling
The middle and high dosage group of the ethanol extract of Cheng forture paulownia root 95%（20,40g/kg）Compare with model group, mice ear swelling shows
Writing reduces, and difference has conspicuousness（P ＜ 0.01）.Qi ethanol extract high dose group of Zhong Cheng forture paulownia root 95% suppresses to mice ear
Rate is most notable, and up to 47.61%.The results are shown in Table 3.
The ethanol extract paraxylene of 3 Cheng paulownia of table 95% causes the impact of mice auricle swelling
Note：Compare with model group, * * * P ＜ 0.001, * * P ＜ 0.01.
The impact of d Dichlorodiphenyl Acetate induced mices abdominal cavity capillary permeability
The middle and high dosage group of the ethanol extract of Cheng forture paulownia root 95%（20,40g/kg）Compare with model group, significantly reduce little caused by acetic acid
Mouse abdominal cavity capillary permeability, difference has conspicuousness（P ＜ 0.05）.Caused by the ethanol extract Dichlorodiphenyl Acetate of Ti Shi Cheng forture paulownia root 95%
Mouse peritoneal capillary transparency increase significantly inhibits effect, the results are shown in Table 4.
The impact of the ethanol extract Dichlorodiphenyl Acetate induced mice abdominal cavity capillary permeability of 4 Cheng paulownia of table 95%
Note：Compare with model group, * * * P ＜ 0.001, * P ＜ 0.05.
As a result show, the prepared ethanol extract of Cheng forture paulownia root 95% can be to the swollen tissue of inflammation early stage in embodiment
There is significant inhibitory action, can be used to prepare anti-inflammatory medicaments with the ethanol extract of antiinflammatory action ， Cheng forture paulownia root 95%.
Claims (4)
1. the preparation method of the ethanol extract of Cheng forture paulownia root 95%, it is characterised in that comprise the steps：
（1）Cheng forture paulownia root is crushed, 80% ethanol solution refluxing extraction of 8 times of amounts 3 times, each 2h, temperature 70 C is filtered, and merges filter
Liquid, filtrate puts Rotary Evaporators decompression recycling ethanol, and concentrate puts water-bath Back stroke ethanol solution to the greatest extent, and Gan Zao get Cheng forture paulownia root ethanol is total
Extract；
（2）Jiang Cheng forture paulownia root ethanol total extract medicinal extract, after being dissolved in water, extracted 5 times with isopyknic 95% ethanol, merged each time
Extract, after recycling design, get is Dao the ethanol extract of Cheng forture paulownia root 95%.
2. the application of the ethanol extract of claim 1 Suo Shu Cheng forture paulownia root 95%, it is characterised in that：In anti-inflammatory medicaments are prepared
Application.
3. the application of the ethanol extract of claim 2 Suo Shu Cheng forture paulownia root 95%, it is characterised in that：Preparing anti-inflammation medicine
The application of thing.
4. the application of the ethanol extract of claim 2 Suo Shu Cheng forture paulownia root 95%, it is characterised in that：Preparing anti-chronic inflammation medicine
Application in thing.
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| CN201611254326.2A CN106620045A (en) | 2016-12-30 | 2016-12-30 | Preparation method and application of 95% ethanol extract of clerodendrum japonicum roots |
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| CN201611254326.2A CN106620045A (en) | 2016-12-30 | 2016-12-30 | Preparation method and application of 95% ethanol extract of clerodendrum japonicum roots |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113648356A (en) * | 2021-09-14 | 2021-11-16 | 广西国际壮医医院 | Clerodendrum petasitum extract and application thereof in preparation of anti-inflammatory composition |
-
2016
- 2016-12-30 CN CN201611254326.2A patent/CN106620045A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 秦祖杰等: "赪桐根的化学成分预试验研究", 《广西中医学院学报》 * |
| 陈俊等: "赪桐根水提物抗炎作用研究", 《广西中医药大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113648356A (en) * | 2021-09-14 | 2021-11-16 | 广西国际壮医医院 | Clerodendrum petasitum extract and application thereof in preparation of anti-inflammatory composition |
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Application publication date: 20170510 |
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