CN106615849A - Piglet compound feed containing antimicrobial peptide for later stage of nursery and preparation method of piglet compound feed - Google Patents

Abstract

The invention discloses a piglet compound feed containing antimicrobial peptide for the later stage of nursery and a preparation method of the piglet compound feed. The compound feed is prepared from the following components in parts by weight: 58 to 61 parts of maize, 14 to 16 parts of bean pulp, 8 to 12 parts of imported DDGS (Distillers Dried Grains with Soluble), 5 to 8 parts of fermented soybean meal, 2 to 4 parts of glucose, 1.8 to 5 parts of imported fish meal, 0.4 to 0.8 part of calcium hydrophosphate, 0.15 to 0.2 part of lysine, 0.1 to 0.4 parts of table salt, 0.8 to 1.0 part of stone powder, 0.15 to 0.2 part of composite trace element premix, 0.10 to 0.15 part of rice bran, 0.08 to 0.10 part of methionine, 0.05 to 0.10 part of threonine, 0.03 to 0.10 part of choline, 0.02 to 0.05 part of composite multivitamin premix, 0.02 to 0.05 part of antioxidant, 0.01 to 0.03 part of plectasin antibacterial peptide, 0.01 to 0.03 part of cecropin and 0.02 to 0.05 part of plant essential oil complex. Proved by experiments, the antibacterial peptide feed disclosed by the invention can obtain body weight which is slightly higher than that of an antibiotic group and better feed rewards and has no influence on metabolism of piglets, body tissue proteinosis and an immunologic function. The piglet compound feed has important economic value and social value for ensuring piglet growth, intestinal health and food safety of human beings.

Description

A kind of child care later stage compound feed for piglets containing antibacterial peptide and preparation method thereof

Technical field

The present invention relates to animal nutrition and feed technical field, and in particular to a kind of child care later stage containing antibacterial peptide uses piglet Mixed feed and preparation method thereof, the feed is adapted to weaned piglet and starts feeding after one month.

Background technology

Wean in nature typically occurs in or so monthly age of piglet 3.In actual production, in order to improve sow productivity and To piglet vertical transmission, pig farm is usually chosen in week old of piglet 3 or so wean to utilization rate, the reduction parent of childbirth house.However, this When piglet digestive system not yet develop perfect, gastric acid secretion is not enough, digestive organs volume and weight is all smaller, various digestive ferments Activity is relatively low.Additionally, early-weaned pigletses not yet set up stable intestinal microflora, flora type and quantity easily change. Indigested nutriment enters hindgut, can be as the nutriment of hind microflora propagation, so as to promote a large amount of of microorganism Breeding, causes intestinal microecology disorderly, ultimately results in grice diarrhoea and disease takes place frequently.

Past, people thought always, and low-level antibiotic is added in animal diets to be prevention disease, improve animal intestines and stomach The effective measures of road health, and then can improve food conversion ratio and promote to grow.But use in a large number for a long time in animal feed Antibiotic, can not only increase drug Resistance of Pathogenic Microorganism from Surface, can also cause microbial population of animal intestinal tract disorder, gut barrier function to weaken, Affect the stable state of enteron aisle so as to destroy the congenital immunity of body, finally reduce breeding performonce fo animals.Research shows, routinely kills Dead weaker bacterium, can cause stronger bacteria living to get off and breed without restraint, produce the mankind uncontrollable " superbacteria ". More seriously, long-term a large amount of addition antibiotic in animal feed, cause antibiotic residue to increase, increase the mankind via food Antibiotic resistance, it is serious to threaten human health and pollute environment.So, in the world many developed countries all have begun to strictly Livestock products examination criteria is limited using antibiotic and improves, research and development new high-efficiency Substitutes For Antibiotic has important scientific meaning And social value.Antibacterial peptide (Antibacterial Peptides), is called antimicrobial peptide (Antimicrobial Peptides) or host defense peptide (Host Defense Peptides), it is that a class is widely present in nature biotechnology body Small peptide material, it is the important component part of body innate immune system.Due to antibacterial peptide to bacterium, fungi, parasite, The extensive inhibitory action such as virus, tumour cell, and drug resistance is not likely to produce, it is outstanding in numerous substitute antibiotics products Person, has a good application prospect.

The content of the invention

It is an object of the invention to provide a kind of nursery piglet compound feed containing antibacterial peptide and preparation method thereof, solves to carry High Production Performance of Weaning Pigs and add the problem of antibiotic in a large number, and then ensure charcuterie safety and human health.

In order to achieve the above object, the technical scheme of present invention offer is：

The child care later stage containing antibacterial peptide includes the component of following weight portion with compound feed for piglets：

Corn 58-61 parts, dregs of beans 14-16 parts, import DDGS8-12, fermented bean dregs 5-8 parts, glucose 2-4 parts, import fish It is powder 1.8-5 parts, stone flour 0.8-1.0 parts, calcium monohydrogen phosphate 0.4-0.8 parts, lysine 0.15-0.2 parts, salt 0.1-0.4 parts, compound Microelement pre-mix material 0.15-0.2 parts, rice bran 0.10-0.15 parts, methionine 0.08-0.10 parts, threonine 0.05-0.10 parts, Choline 0.03-0.10 parts, compound vitamin premix 0.02-0.05 parts, antioxidant 0.02-0.05 parts, cecropin antimicrobial peptides 0.01-0.03 parts, plectasin antibacterial peptide 0.01-0.03 parts, plants essential oil compound 0.02-0.05 parts.

The compound method of above-mentioned antibacterial peptide feed comprises the steps：

(1) corn, dregs of beans, fermented bean dregs, import DDGS, imported fish meal, glucose, rice bran are crushed respectively, prepares mesh The various powders of 1000 μm of particle diameter of mark, the average grain diameter for controlling powder is 930-1000 μm as qualified.Suitable grinding particle size The conversion ratio of feed is remarkably improved, animal wastes excretion is reduced, the production performance of animal is improved.By Sizing test, protect Uniform, the promotion piglet digestion of card feed granules.Remaining raw material because itself granularity is already less than 1000 μm, without the need for crush.

(2) bulk raw material and small powder are weighed respectively in the ratio described in claim 1, bulk raw material is corn, dregs of beans, sends out Ferment dregs of beans, import DDGS, imported fish meal, glucose, remaining is small powder；

(3) load weighted bulk raw material and small powder are mixed, obtains final product the child care later stage compound feed for piglets containing antibacterial peptide.

Below the invention will be further described：

Antibacterial peptide, also known as antimicrobial peptide, can be by the natural product such as bacterium, plant, insect, amphibian, mammal It is raw, and the bioactive molecule with innate immune reaction, play cellular host defense effect by invading pathogenic microorganism. Antibacterial peptide has broad spectrum antibiotic activity, low toxicity and low drug resistance, while being considered as a kind of as higher organism immunomodulator Future has future and potential drug candidate, belongs to environment-friendly type antibiotic.

Cecropin is first antibacterial peptide being found in the world, and Sweden scientist used Bacillus cercus in 1981 (Bacillus cereus) induction is cherished guppy giant silkworm (Hyalophora cecropia) and generates Antimicrobially active polypeptides material, fixed Entitled Cecropin.There is the cecropin antimicrobial peptides that the present invention is applied broad-spectrum antibacterial to act on, but main to Gram-negative Bacterium is worked, and it has following architectural feature：(1) it is made up of 31-39 amino acid residue, molecular weight is about 4KD, without half Guang Propylhomoserin, it is impossible to form intramolecular disulfide bond.This simple peptide chain structure is convenient for chemical synthesis or gene engineering expression, And ensure product stability.(2) there is alkaline N-terminal, N-terminal region is rich in hydrophilic-basic amino acids residue, can be formed almost Perfect parents' helical structure, this is beneficial to cecropin and is adsorbed onto on electronegative bacterial membrane.(3) C-terminal is dredged containing more Aqueous amino acid residue, can form hydrophobic helices, and hydrophobic afterbody is conducive to antibacterial peptide to insert the bilayer lipid membrane of bacterial membrane In.And its C-terminal is amidated, amidatioon has important function to its antibacterial activity.(4) two ends of molecule respectively form an amphiphilic Property α spirals, this α spirals are the primary structures for destroying, cracking bacterium.

Plectasin (Plectasin) is that the research groups such as Mygind passed through the false black disk of the saprophytic sac fungus of examination in 2005 The cDNA storehouses of bacterium (Pseudoplectania nigrella) secretory protein, therefrom find out some with invertebrate alexin sequence The highly similar fragment of row, and the first mycophylaxin that screening is separated.It has following architectural feature：(1) mycelia is mould Element is a class endogenous, the highly stable, antibacterial peptide rich in cysteine, typically there is 6-8 Conserved cysteines in its sequence 3-4 is formed to disulfide bond.Disulfide bond logarithm and connected mode have important work in terms of alexin structure and antibacterial functions are maintained With present invention plectasin used forms intrachain disulfide bond to stablize its 2 antiparallel β-pleated sheets by molecular modification technology Piece and 1 α spiral are so as to improving bacteriostatic activity, strengthen stability.(2) plectasin has good salt ion tolerance, In physiology salt ionic concentration, similarly hereinafter sample has bactericidal activity.(3) additionally, it also has good pH stability and thermally-stabilised Property, in the range of pH value 2.0-10.0, plectasin has bacteriostatic activity to staphylococcus aureus；After 100 DEG C of heat treatment 1h, Also papain and pepsic degraded can be resisted.(4) plectasin is combined with cell wall precursors thing lipid II, is caused thin Cell wall synthesis is disorderly, destroys the complete structure of cell membrane, forms hole, and content leaks, whole cell lysis is dead.In vitro Experimental study reports that plectasin is mainly to G⁺Several species of (gram-positive bacteria) are (including streptococcus, staphylococcus Category, enterococcus spp, corynebacterium and shaft-like Pseudomonas) there is good lethal effect, especially to streptococcus pneumonia (S.pneumoniae) MIC is only 0.125mg/l, suitable with the sterilized speed of vancomycin and penicillin.

Plant extracts be with plant as raw material, through series of physical, chemical extraction process, obtain by a kind of or many Plant the plant product of active skull cap components composition.Plant extracts is in history always as the medicine or nutritious supplementary pharmaceutical of the mankind Use in a large number, there is thousands of years history.In recent years, research of the plant extracts in Swine Production in terms of substitute antibiotics is achieved Good result.Containing abundant bioactivator in plant extracts, including thymol, carvacrol, cinnamic acid etc., these Material is by suppressing harmful microorganism, adjusting health and growth that the effects such as immune, anti-oxidant and regulation metabolism promote pig.In vitro Research shows, plant extracts for there is antibacterial action including the various pathogens including mould, the activity of its main antibacterial Material is phenolic compound.

Aldehydes matter such as Thymol, carvacrol, thymol, the composition that these contain phenol type structure all shows stronger Antibacterial action.The antibiotic property of aldehydes matter is affected by its own configuration and contained functional group, wherein the work of oh group With very big, its quantity and the relative position on phenol ring can affect the anti-microbial property of aldehydes matter, and thymol and carvacrol are to leather Lan Shi positive bacterias are different with the fungistatic effect of Gram-negative bacteria, and can all strengthen phenols containing alkyl or acetate on phenol ring The antibacterial activity of material, these functional groups can make protein inactivation by the oxidation to coloured glaze base；With the compound such as skin containing coloured glaze base There are Competitive assays or directly suppress the normal physiological function of some enzymes in propylhomoserin, make pathogen metabolic disorder, metabolic to obtain To suppression, antibacterial purpose is finally reached.In addition, plant extracts makes the phosphatide on cell membrane and mitochondria using itself hydrophobicity Structure separates, and destroys eucaryotic cell structure, strengthens permeability of cell membrane, causes the leakage of intracellular ion and other materials, finally leads Cause the death of bacterium.

In-vitro simulated small intestine condition research finds that 4 kinds of plant additives are followed successively by the colibacillary antibiotic property of enteron aisle：Meat Osmanthus ＞ carvacrol ＞ thymol ＞ eugenols.Cinnamic acid has volatile, oxidizable characteristic, is difficult to carry out preservation.By meat Cinnamic aldehyde is combined with other materials, can be more easy to maintain and strengthens fungistatic effect.Additionally, antibacterial experiment in vitro shows, cinnamic acid pair Aspergillus flavus, aspergillus fumigatus and aspergillus niger etc. all have bacteriostasis.Cinnamic acid is mainly manifested in the defence for improving host to pathogen Property, suppress conidia germination and formation, cause conidium to be cleared up, cause mycelium morphology to produce abnormal, long-time Interference thalli growth.

The standard and detailed step of preparation method is as follows：

(1) purchasing of raw materials, examination and inspection：First purchasing of raw materials standard is formulated to various raw materials needed for formula, it is stipulated that each The parameter area of nutritive index；Secondly, get a good buy by shopping around and select the supplier for having good qualification to carry out the comprehensive of material quality and price Composition and division in a proportion compared with, then decide whether buying；Then, according to feedstuff procurement criteria, the raw material to purchasing carries out quick visual examination With laboratory chemical analysis, quick visual examination is qualified can be received, unqualified, rejected；The qualified raw material of visual examination, Proceed laboratory chemical analysis, qualified warehouse-in, the underproof return of goods.By this program, material quality is strictly controlled.

(2) crush and grinding particle size inspection：By corn, dregs of beans, fermented bean dregs, import DDGS, imported fish meal, glucose, Rice bran is crushed respectively using beater disintegrating machine, prepares the various powders of 1000 μm of target grain size.It is aobvious with optics after crushing Micro mirror does rough detection, and the particle diameter obtained by making is substantially close to target grain size.Then laser particle size analyzer is used (NICOMPTM380 types, the U.S.) determines the average grain diameter of various raw materials, and average grain diameter is as qualified at 930-1000 μm.Suitably Grinding particle size be remarkably improved the conversion ratio of feed, reduce animal wastes excretion, improve the production performance of animal.By grain Degree inspection, it is ensured that uniform, the promotion piglet digestion of feed granules.Remaining raw material because itself granularity is already less than 1000 μm, nothing Need to crush.

(3) weighing of bulk raw material：Weigh operation and generally comprise two parts：One is the less original of the additions such as premix Material is weighed, and two is the weighing of bulk raw material.To pursue proportioning accuracy, saving manpower, bulk raw material generally adopts automatic weighing.Press According to components by weight percent, these bulk raw materials of corn, dregs of beans, fermented bean dregs, import DDGS, imported fish meal, glucose are using minimum point The automatic weighing device of angle value 0.10kg is weighed.

(4) weighing of small powder：Small powder is because addition is little, wide in variety, easy caking, storehouse easy to stick, chemical reaction etc. easily occurs Reason, still weighs mode using manual.According to components by weight percent, calcium monohydrogen phosphate, stone flour, salt, rice bran, lysine, methionine, Threonine, choline, microelement pre-mix material, vitamin premix, antioxidant, antibacterial peptide and plants essential oil compound etc. add The low small powder of amount is weighed manually using the electronic scale of minimum division value 0.01kg.

(5) mix：Load weighted small powder and bulk raw material are mixed into 10min in mixer.Grinding particle size is more uniform, mixes The conjunction time is longer, and the uniformity is better.Require the mixed feed mixture homogeneity coefficient of variation≤10%.With reference to GB/T 5918-2008 The measure of feed product mixture homogeneity, every half a year carries out mixed once uniformity inspection.

(6) pack：Feed after being well mixed, is completed to weigh, is packed, package by automatic packing machine.Product packaging is 20kg/ bags.

(7) proportioning accuracy requires control within 0.1%.For example, if mixer capacity is 1000kg, then set Meter prepares 1000kg feeds, and final dosage acceptability limit is 999-1001kg after the completion of dispensing.

The antibacterial peptide feed of the present invention can reach the growth performance same with antibiotic group, and the metabolism to piglet, machine Body tissue proteinosis and immunologic function do not have any impact.This ensures human food to ensureing piglet growth and intestinal health Safety has important economic worth and social value.

Specific embodiment

Embodiment 1

58 parts of corn, 14 parts of dregs of beans, import DDGS8 parts, 8 parts of fermented bean dregs, 4 parts of glucose, 5 parts of imported fish meal, stone flour 1 Part, 0.8 part of calcium monohydrogen phosphate, 0.15 part of lysine, 0.4 part of salt, 0.15 part of rice bran, 0.15 part of composite trace element premix, 0.1 part of methionine, 0.05 part of threonine, 0.03 part of choline, 0.05 part of compound vitamin premix, 0.05 part of antioxidant, mycelia 0.01 part of mycin antibacterial peptide, 0.01 part of cecropin antimicrobial peptides, 0.05 part of plants essential oil compound.

The compound method of above-mentioned antibacterial peptide feed comprises the steps：

(1) purchasing of raw materials, examination and inspection：Various raw materials needed for formula are adopted with reference to purchasing of raw materials standard first Purchase；The qualified reception of visual examination after raw material delivery, underproof rejection；The qualified warehouse-in of chemical analysis, the underproof return of goods.

(2) crush and grinding particle size inspection：By corn, dregs of beans, fermented bean dregs, import DDGS, imported fish meal, glucose, Rice bran is crushed respectively using beater disintegrating machine, prepares the various powders of 1000 μm of target grain size.It is aobvious with optics after crushing Micro mirror does rough detection, and the particle diameter obtained by making is substantially close to target grain size.Then laser particle size analyzer is used (NICOMPTM380 types, the U.S.) determines the average grain diameter of various raw materials, and average grain diameter is as qualified at 930-1000 μm.

(3) weighing of bulk raw material：Using the automatic weighing device of minimum division value 0.10kg, according to components by weight percent, claim 58 parts of corn of amount, 14 parts of dregs of beans, 8 parts of fermented bean dregs, import DDGS8 parts, 5 parts of imported fish meal, 4 parts of glucose, 1 part of stone flour.

(4) weighing of small powder：According to components by weight percent, phosphoric acid hydrogen is weighed manually using the electronic scale of minimum division value 0.01kg 0.8 part of calcium, 0.15 part of lysine, 0.4 part of salt, 0.15 part of rice bran, 0.15 part of composite trace element premix, methionine 0.1 Part, 0.05 part of threonine, 0.03 part of choline, 0.05 part of compound vitamin premix, 0.05 part of antioxidant, plectasin antibacterial peptide 0.01 part, 0.01 part of cecropin antimicrobial peptides, 0.05 part of plants essential oil compound.

(5) mix：Load weighted bulk raw material and small powder are mixed into 10mim in mixer.

(6) pack：Feed after being well mixed, is completed to weigh, is packed, package by automatic packing machine.Product packaging is pressed 20kg/ bags.

(7) proportioning accuracy requires control within 0.1%.For example, if preparing 100 parts of feed, then dispensing After the completion of final dosage acceptability limit should be 99-101 parts.

Embodiment 2

61 parts of corn, 16 parts of dregs of beans, import DDGS12 parts, 5 parts of fermented bean dregs, 2 parts of glucose, 1.8 parts of imported fish meal, stone 0.8 part of powder, 0.4 part of calcium monohydrogen phosphate, 0.2 part of lysine, 0.1 part of salt, 0.2 part of composite trace element, 0.1 part of rice bran, egg ammonia 0.08 part of acid, 0.1 part of threonine, 0.1 part of choline, 0.02 part of compound vitamin, 0.02 part of antioxidant, plectasin antibacterial peptide 0.03 part, 0.03 part of cecropin antimicrobial peptides, 0.02 part of plants essential oil compound.

The compound method of above-mentioned antibacterial peptide feed comprises the steps：

(1) purchasing of raw materials, examination and inspection：Various raw materials needed for formula are adopted with reference to purchasing of raw materials standard first Purchase；The qualified reception of visual examination after raw material delivery, underproof rejection；The qualified warehouse-in of chemical analysis, the underproof return of goods.

(2) crush and grinding particle size inspection：By corn, dregs of beans, fermented bean dregs, import DDGS, imported fish meal, glucose, Rice bran is crushed respectively using beater disintegrating machine, prepares the various powders of 1000 μm of target grain size.It is aobvious with optics after crushing Micro mirror does rough detection, and the particle diameter obtained by making is substantially close to target grain size.Then laser particle size analyzer is used (NICOMPTM380 types, the U.S.) determines the average grain diameter of various raw materials, and average grain diameter is as qualified at 930-1000 μm.

(3) weighing of bulk raw material：Using the automatic weighing device of minimum division value 0.10kg, according to components by weight percent, claim 61 parts of corn of amount, 16 parts of dregs of beans, import DDGS12 parts, 5 parts of fermented bean dregs, 2 parts of glucose, 1.8 parts of imported fish meal.

(4) weighing of small powder：According to components by weight percent, stone flour is weighed manually using the electronic scale of minimum division value 0.01kg 0.8 part, 0.4 part of calcium monohydrogen phosphate, 0.2 part of lysine, 0.1 part of salt, 0.1 part of rice bran, 0.2 part of composite trace element, methionine 0.08 part, 0.1 part of threonine, 0.1 part of choline, 0.02 part of compound vitamin, 0.02 part of antioxidant, plectasin antibacterial peptide 0.03 Part, 0.03 part of cecropin antimicrobial peptides, 0.02 part of plants essential oil compound.

(5) mix：Load weighted bulk raw material and small powder are mixed into 10mim in mixer.

(6) pack：Feed after being well mixed, is completed to weigh, is packed, package by automatic packing machine.Product packaging is pressed 20kg/ bags.

(7) proportioning accuracy requires control within 0.1%.For example, if preparing 100 parts of feed, then dispensing After the completion of final dosage acceptability limit should be 99-101 parts.

Experiment place：The Institute of Subtropical Agriculture, The Chinese Academy of Sciences (Changsha, Hunan)

Experimental period：In August, 2016 in September, -2016

Zoopery designs (being shown in Table 1)：From body condition is in good health, body weight size is close to, has weaned one month or so " Du × long × greatly " three way cross weanling pig 42, average weight is 17.34kg or so, and male and female half and half is randomly divided into 3 Group, 14 repetitions of antibiotic-free group, 14 repetitions of antibiotic group, 14 repetitions of substitute antibiotics group are fed using single hurdle, each Repeat 1 pig.Antibiotic-free group is negative control group, and feeding basal diet does not add any antibiotic；Antibiotic group is just right According to group, basal diet+antibiotic is fed；Substitute antibiotics group is experimental group, and feeding basal diet+antibacterial peptide and plants essential oil are carried Take thing.Test full period 14d.

Table 1

Test pig daily nursing：According to pig farm regulation immunity and expelling parasite.Test is carried out in totally-enclosed pig house, and piglet is raised Support and pulled in half crack, control temperature 28-30 DEG C, free choice feeding and drinking-water feed 4 times daily that (time is respectively 7:30、11: 30、15:30 and 19:30), whetheing there is clout in observation feed trough before feeding every time carries out suitably adjusting to ensure its free choice feeding, from By drinking water.Every morning, afternoon respectively sweep colony house 1 time in experimental period.

Testing index and method

The measure of growth performance

Two stages are weighed respectively in the beginning and end phase in units of repetition, and count feed consumption, are calculated Average daily gain, average daily gain and feed-weight ratio and diarrhea rate and Scours index.

Average daily gain：From experimental period, the head number of the daily feed consumption of each treatment group and pig is recorded, arrived Calculate during off-test：Average daily gain=pig's head number/experiment the number of days of feed intake * experiment daily；Average daily gain:From test Phase, record the original body mass of every pig and record pig number, obtain average starting weight；Every pig is weighed and counts during off-test Calculate average end weight, (latter stage body weight-original body mass)/(test pig's head number * number of days=average daily gain.Feed-weight ratio=average day Feed intake/average daily gain.

Diarrhea rate：In experiment periods, record per the daily grice diarrhoea head number of times for the treatment of group and diarrheal scores, calculate piglet abdomen Rate is rushed down, formula is：All grice diarrhoea head number of times/(number of days of test × test pig head number) in diarrhea rate=experimental period × 100%；All grice diarrhoeas scoring sum/(number of days of test × test pig head number) in Scours index=experimental period.Diarrhoea Standards of grading are shown in Table 2, and growth performance and diarrhoea situation the results are shown in Table 3：

Table 2

Table 3

Raising experiment result shows：Child care piglet latter stage average weight experimental group is slightly above positive control group, illustrates using anti- Bacterium peptide substitute antibiotics do not affect piglet growth performance, can reach the level of growth as addition antibiotic；From feedstuff-meat ratio From the point of view of, experimental group also obtain the preferable price of deed, and this has further affirmed that the scheme of substitute antibiotics treatment group completely may be used OK.Child care grice diarrhoea rate and Scours index, positive control group can effectively suppress young less than negative control group profile addition antibiotic Diarrhea of pigs, and experimental group grice diarrhoea rate and Scours index are slightly above positive and negative control group, illustrating the research of substitute antibiotics still has Certain progressive space.The measurement results such as Biochemical Indices In Serum, immune indexes are shown in Table 4：

Table 4:

Under normal circumstances, animal blood glucose concentration relative constancy.Blood sugar is that the main energy of animal body is originated, animal body Mainly glucose is obtained by intestinal absorption.Whether just blood-sugar content can to a certain extent reflect animal body function carbohydrate metabolism Often.The Main Function of total serum protein is to participate in the transhipment of nutriment, keep the dynamic equilibrium of histone, maintain blood PH value etc..The concentration of total serum protein can effectively reflect the anabolic power of body protein, total protein concentration of serum liter Height explanation body is carried out to the direction of tissue proteinosis, the growth and development of energy promotion organization organ.Immunoglobulin (Ig) is main In being present in serum, it can play immune response and protect body with external specific antigen.Immunoglobulin IgG and IgM are The height of a main antibody-like, Immunoglobulin IgG and IgM contents can to a certain extent reflect the immunity of animal in blood Performance.The main Physiological Function of growth hormone is to promote the every other tissue growth beyond nerve fiber；Promote body synthesis Metabolism and protein synthesize；Promote lipolysis.Insulin-like growth factor-i is the multi-functional regulation of cell proliferation factor of a class. Differentiation, propagation in cell, it is individual grow in there is important facilitation.This experiment, Portugal in child care piglet serum Grape sugared content, total protein content, serum IgG, IgA and IgM contents, growth hormone content and insulin-like growth factor-Ⅱ content, Each group illustrates to add in feed antibacterial peptide and plants essential oil extract substitute antibiotics to child care piglet substantially in same scope Metabolism, body tissue proteinosis and immunologic function do not affect.

Can be seen that from above experimental result and compare with antibiotic group, antibacterial peptide and plant are added in child care piglet diet Extraction of essential oil thing substitute antibiotics, it is possible to obtain slightly above the body weight of antibiotic group and the more preferable price of deed, to diarrhea rate and Scours index does not make significant difference, and the metabolism, body tissue proteinosis and immunologic function on piglet does not also affect.Antibacterial peptide and Plants essential oil extract substitute antibiotics are feasible.

Claims (2)

1. a kind of child care later stage compound feed for piglets containing antibacterial peptide, it is characterised in that the mixed feed includes following weight The component of amount part：

Corn 58-61 parts, dregs of beans 14-16 parts, import DDGS8-12, fermented bean dregs 5-8 parts, glucose 2-4 parts, imported fish meal It is 1.8-5 parts, calcium monohydrogen phosphate 0.4-0.8 parts, lysine 0.15-0.2 parts, salt 0.1-0.4 parts, stone flour 0.8-1.0 parts, compound micro- Trace mineral feed 0.15-0.2 parts, rice bran 0.10-0.15 parts, methionine 0.08-0.10 parts, threonine 0.05-0.10 parts, courage Alkali 0.03-0.10 parts, compound vitamin premix 0.02-0.05 parts, antioxidant 0.02-0.05 parts, plectasin antibacterial peptide 0.01-0.03 parts, cecropin antimicrobial peptides 0.01-0.03 parts, plants essential oil compound 0.02-0.05 parts.

2. the preparation method of mixed feed as claimed in claim 1, it is characterised in that methods described comprises the steps：

(1) corn, dregs of beans, fermented bean dregs, import DDGS, imported fish meal, glucose, rice bran are crushed respectively, prepares target grain The various powders in 1000 μm of footpath, the average grain diameter for controlling powder is 930-1000 μm as qualified.Suitable grinding particle size can show The conversion ratio for improving feed is write, animal wastes excretion is reduced, the production performance of animal is improved.By Sizing test, it is ensured that raise Uniform, the promotion piglet digestion of material particle.Remaining raw material because itself granularity is already less than 1000 μm, without the need for crush.

(2) bulk raw material and small powder are weighed respectively in the ratio described in claim 1, bulk raw material is corn, dregs of beans, fermentation beans The dregs of rice, import DDGS, imported fish meal, glucose, remaining is small powder；

(3) load weighted bulk raw material and small powder are mixed, obtains final product the child care later stage compound feed for piglets containing antibacterial peptide.