CN106612737A - Verification method for saline-alkaline resistance of paddy rice - Google Patents
Verification method for saline-alkaline resistance of paddy rice Download PDFInfo
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- CN106612737A CN106612737A CN201611173509.1A CN201611173509A CN106612737A CN 106612737 A CN106612737 A CN 106612737A CN 201611173509 A CN201611173509 A CN 201611173509A CN 106612737 A CN106612737 A CN 106612737A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
Abstract
The invention discloses a verification method for saline-alkaline resistance of paddy rice. The verification method comprises the following steps: (1) preparing a saline-alkaline solution; (2) carrying out seed germination treatment; (3) observing and recording a germination result; (4) computing a result, and comparing a computing result with a relative saline/alkaline damage rate grading standard table, thus verifying the strength of the saline-alkaline resistance of the paddy rice. According to the verification method disclosed by the invention, the saline-alkaline resistance of paddy rice varieties can be verified, so that basis is provided for later research as well as development and utilization of seed quality resources of the paddy rice; the verification method is simple and easy in operation, the verification result is reliable, and the standard is high.
Description
Technical field
The present invention relates to crops Saline alkali tolerance probes into technical field, more particularly, to a kind of Rice Salt alkalescence
Authentication method.
Background technology
Soil salt is excessive, and to the harm that crop is caused salt damage is claimed；The salinity of salt stress mainly has NaCl, Na2SO4、
Na2CO3、NaHCO3, NaCl and Na2SO4Claim solonchak, Na when dominant2CO3And NaHCO3Claim alkaline earth, normal conditions when dominant
Under, this several salt exists simultaneously, referred to as saline-alkali soil.The salinization of soil is worldwide environmental problem, in the world about 20%
Arable land and 50% crop soil are subject to salt stress, take up an area the 6% of ball top, the hm of saline and alkaline land area about 1,000,000,0002.China
Nearly 100,000,000 hm of saline alkali land area2, it is mainly distributed on northwest, North China, northeast and coastal area.
At 0.2%～0.5%, the growth to crop has a negative impact generally soil salt content, reach 0.6%～
When 10%, grievous injury plant destroys soil texture, and to agricultural production serious harm is brought.
China is large agricultural country, is also populous nation, solves the problems, such as that food supply is very urgent.A central text in 2015
Part is explicitly pointed out：" implementing alkaline land improvement science and technology demonstration, constantly strengthen grain production capacity ", while in the planning of country 13
One of 14th NCCCP strategy is that " hiding grain in ground, Tibetan grain in skill strategy " all specify that reasonable necessity that grain is produced using salt-soda soil
Property.Meanwhile, it is to mitigate one of effective ways that the salinization of soil endangers to plant salt tolerant crop kind.Paddy rice is used as important grain
Crop, is to be adapted to one of crop that salt-soda soil is planted, and finds the strong rice pest insects of Saline alkali tolerance, cultivates and is adapted to salt-soda soil plantation
Rice varieties, are using the guarantee condition of salt-soda soil kind rice, while being also beneficial to vast salt-soda soil region peasant's increasing both production and income.
The content of the invention
For problems of the prior art, it is an object of the invention to provide a kind of identification side of Rice Salt alkalescence
Method, to utilizing for the research and development of the later stage of Rice Germplasm Resources foundation is provided.
For achieving the above object, the technical solution adopted in the present invention is：A kind of authentication method of Rice Salt alkalescence, its
It is characterised by, the authentication method is comprised the following steps：
Step (1)：Prepare saline solution：A, prepare mass concentration gradient be 0.3%, 0.4%, 0.5%, 0.6%,
0.7%th, 0.8%, 0.9%, 1.0% NaCl salting liquids；B, prepare mass concentration gradient be 0.05%, 0.10%, 0.15%,
0.20%th, 0.25%, 0.30%, 0.35%, 0.40% Na2CO3Aqueous slkali；
Step (2)：Germination is processed：Some full rice paddy seeds are selected, is fully soaked in 20～25 DEG C of clear water
72～75h is until show money or valuables one carries unintentionally, then seed, to broken chest, is then uniformly placed in and is covered with bilayer by vernalization under 25～30 DEG C of constant temperatures
In the germination box of filter paper, the saline and alkaline concentration gradient solution prepared in step (1) is separately added into germination box immersion treatment as
Treatment group, it is equal with corresponding solution to observe humidity condition in each germination box as a control group, daily with clear water immersion filter paper
Even equivalent supplement is ensureing humidity；
Step (3)：Observe and record germination result：Observation seed sprouting situation, with the bud length of seed seed length is equal to
Half, to be equal to seed length be germination standard to root length, and the germinative number for recording seed is investigated every 24h, and measures height of seedling, germinate
Germinating energy is calculated within 4th day, germination percentage is calculated within the 10th day；
Step (4)：As a result computing formula：
Germinating energy (%)=(the 4th day accumulative germination grain number/confession examination grain number) × 100；
Germination percentage (%)=(the 10th day accumulative germination grain number/confession examination grain number) × 100；
Germination index (GI)=∑ (Gt/Dt), wherein：Gt：T day germinative numbers, Dt：Germination number of days；
With respect to salt/alkali evil rate (%)=[(control group germination percentage-treatment group germination percentage)/treatment group germination percentage] × 100；
By the way that result of calculation is strong and weak to identify the Saline alkali tolerance of paddy rice with reference to relative salt/alkali evil rate grade scale table.
The beneficial effects of the present invention is：The germinating energy for passing through the calculating of paddy rice germination test in saline solution of the invention,
The Seed Germination of Rice phase such as germination percentage, germination index, height of seedling Germination Characteristics and relative salt/alkali evil rate identifying the Saline alkali tolerance of paddy rice,
Identify the rice varieties with stronger Saline alkali tolerance, and the Salt And Alkali Tolerance threshold of the clear and definite rice varieties of energy (such as extra large rice 86, Huang Huazhan)
Value, to utilizing for the research and development of the later stage of Rice Germplasm Resources foundation is provided；The extra large rice of acceptable strong Salt And Alkali Tolerance of the invention
86 and the yellow China of Salt And Alkali Tolerance does not account for both typical rice varieties as positive and negative reference object, identify other variety resources of rice
Saline alkali tolerance；The method is simple to operation, and qualification result is reliable, standard is high.
Specific embodiment
Describe the present invention with reference to specific embodiment, the description of this part is only exemplary and explains
Property, there should not be any restriction effect to protection scope of the present invention.
A kind of authentication method of Rice Salt alkalescence of the present invention, the authentication method is comprised the following steps：
Step (1)：Prepare saline solution：A, prepare mass concentration gradient be 0.3%, 0.4%, 0.5%, 0.6%,
0.7%th, 0.8%, 0.9%, 1.0% NaCl salting liquids；B, prepare mass concentration gradient be 0.05%, 0.10%, 0.15%,
0.20%th, 0.25%, 0.30%, 0.35%, 0.40% Na2CO3Aqueous slkali；
Step (2)：Germination is processed：Select some full rice paddy seeds, in 25 DEG C of clear water fully immersion 72～
75h is until show money or valuables one carries unintentionally, then seed, to broken chest, is then uniformly placed in and is covered with sending out for double-layer filter paper by vernalization under 30 DEG C of constant temperatures
In bud box, the saline and alkaline concentration gradient solution prepared in step (1) is separately added into germination box immersion treatment as treatment group, with
With clear water immersion filter paper as a control group, humidity condition in the box of observation germination daily, at the uniform equivalent supplement of corresponding solution
Manage liquid to ensure humidity；
Step (3)：Observe and record germination result：Observation seed sprouting situation, with the bud length of seed seed length is equal to
Half, it is germination standard that root length is equal to seed length, and the germinative number for recording seed is investigated every 24h, and measures height of seedling, is germinateed
Germinating energy is calculated within 4th day, germination percentage is calculated within the 10th day；
Step (4)：As a result computing formula：
Germinating energy (%)=(the 4th day accumulative germination grain number/confession examination grain number) × 100；
Germination percentage (%)=(the 10th day accumulative germination grain number/confession examination grain number) × 100；
Germination index (GI)=∑ (Gt/Dt), wherein：Gt：T day germinative numbers, Dt：Germination number of days；
With respect to salt/alkali evil rate (%)=[(control group germination percentage-treatment group germination percentage)/treatment group germination percentage] × 100；
By the way that result of calculation is strong and weak to identify the Saline alkali tolerance of paddy rice with reference to relative salt/alkali evil rate grade scale table.
In above-mentioned technical proposal, in the step (1), prepare saline and alkaline cross matching solution and make further identification, in setting
Property salinity is：0.7%th, 0.8%, 0.9%, 1.0%, alkaline salinity 0.05%, 0.15%, 0.25%, 0.35% are carried out
Combined crosswise is used as saline and alkaline cross matching solution.According to mono-salt and single alkali test result, saline and alkaline cross matching is designed, for salt tolerant
The further identification for doing Saline alkali tolerance threshold value of the strong or extremely strong kind of alkalescence.
In above-mentioned technical proposal, the paddy rice is extra large rice 86 or Huang Huazhan.
In above-mentioned technical proposal, with extra large rice 86 and the positive and negative Saline alkali tolerances of Huang Hua Zhanwei with reference to rice varieties.
In above-mentioned technical proposal, the salt tolerance threshold range of the extra large rice 86 is 0.9%～1.0%, the alkali resistance threshold of extra large rice 86
Value scope is 0.30%～0.35%；The salt tolerance threshold range that the yellow China accounts for is alkaline-resisting for 0.6%～0.7%, Huang Huazhan's
Property threshold range be 0.15%～0.20%.
In above-mentioned technical proposal, first the seed selected is sterilized with 3% potassium permanganate in the step (2), clear water rinses 2
～3 process.
Embodiment 1
1st, subjects：Extra large rice 86
" extra large rice 86 " is the paddy rice of the energy seawater irrigation that Chen Sheng expert has found and is bred as through M8003 line for many years
New varieties, and in the acquisition of in September, 2014 Ministry of Agriculture Plant new variety protection bulletin.The present embodiment is sent out by the extra large germination period of rice 86
Bud feature to identify its Saline alkali tolerance, further clear and definite kind salt-tolerant threshold, to for the research of the later stage of Rice Germplasm Resources with
Develop and foundation is provided.
2nd, test method：
The preparation of 2.1 saline solutions
It is utilized respectively neutral salt (NaCl) and basic salt (Na2CO3) prepare mass concentration gradient salt, aqueous slkali；Salting liquid
8 gradient salinity (being shown in Table 1) are set, and aqueous slkali arranges 8 gradient alkali concns (being shown in Table 2), in arranging in saline and alkaline cross matching
Property salinity is：0.7%th, 0.8%, 0.9%, 1.0%, alkaline salinity 0.05%, 0.15%, 0.25%, 0.35% is carried out
Combined crosswise (is shown in Table 3).
18 gradient salinity of table
| Process code name | CK | C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 |
| Concentration/% | H2O | 0.3 | 0.4 | 0.5 | 0.6 | 0.7 | 0.8 | 0.9 | 1.0 |
28 gradient alkali concns of table
| Process code name | CK | P1 | P2 | P3 | P4 | P5 | P6 | P7 | P8 |
| Concentration/% | H2O | 0.05 | 0.10 | 0.15 | 0.20 | 0.25 | 0.30 | 0.35 | 0.40 |
The saline and alkaline cross matching of table 3 is combined
2.2 germination tests
The full seed of extra large rice 86 is selected, is sterilized with 3% potassium permanganate, clear water is rinsed 2～3 times, is filled in 25 DEG C of clear water
Sub-dip bubble 72h is until show money or valuables one carries unintentionally, then vernalization, to broken chest, takes 33 germination boxes for being covered with filter paper, wherein 16 under 30 DEG C of constant temperatures
It is separately added into individual germination box in table 1 in 8 gradient salinity solution, tables 2 in 8 gradient alkali concn solution immersion treatments, tables 3
Neutral salinity is arranged in 16 saline and alkaline cross matchings is：0.7%th, 0.8%, 0.9%, 1.0%, alkaline salinity 0.05%,
0.15%th, used as treatment group, the filter paper in 1 germination box soaks conduct with clear water for 0.25%, 0.35% combination for being intersected
Control group, observe daily each germination box in humidity condition, with the uniform equivalent supplement of corresponding solution with ensure germinate box in it is wet
Degree；Observation seed sprouting situation, is equal to the half of seed length, root length and is equal to seed length as germination mark with the bud length of seed
Standard, investigates every 24h and records the germinative number of seed, and measures height of seedling, germinates the 4th day and calculates germinating energy, calculates germination within the 10th day
Rate；
2.3 result computing formula
Germinating energy (%)=(the 4th day accumulative germination grain number/confession examination grain number) × 100；
Germination percentage (%)=(the 10th day accumulative germination grain number/confession examination grain number) × 100；
Germination index (GI)=∑ (Gt/Dt), wherein：Gt：T day germinative numbers, Dt：Germination number of days；
With respect to salt/alkali evil rate (%)=[(control group germination percentage-treatment group germination percentage)/treatment group germination percentage] × 100；
By the way that result of calculation is identified into the Saline alkali tolerance of paddy rice with reference to relative salt/alkali evil rate grade scale table (being shown in Table 4)
It is strong and weak.
Relative salt/alkali evil rate the grade scale of table 4
| Rank | With respect to salt/alkali evil rate % | Salt tolerant/alkalescence |
| 1 | 0～20 | It is extremely strong |
| 3 | 20.1～40 | By force |
| 5 | 40.1～60 | In |
| 7 | 60.1～80 | It is weak |
| 9 | 80.1～100 | It is extremely weak |
3rd, result of the test and data processing
Relevant treatment and statistical analysis are carried out using Excel, DPS statistical software data.
By being calculated germinating energy of the extra large rice 86 in neutral salt solution, germination percentage, germination index, height of seedling, relatively resistance to
Salt damage rate, rank and salt tolerance power are shown in Table 5；Germinating energy of the extra large rice 86 in alkaline salt solution, germination percentage, germination refer to
Number, height of seedling, relatively alkaline-resisting harmful rate, rank and alkali resistance power are shown in Table 6；Saline solution mixing is carried out into saline and alkaline intersection examination
Test, germinating energy of the extra large rice 86 in complex saline-alkali solution, germination percentage, germination index, height of seedling, relatively alkaline-resisting harmful rate, rank and
Alkali resistance power is shown in Table 7.
The extra large result in neutral salt solution of rice 86 of table 5
The extra large result in alkaline salt solution of rice 86 of table 6
The saline and alkaline cross matching of extra large rice 86 of table 7
As shown in Table 5, extra large rice 86 starts to be greatly reduced, compared with CK under neutral salt stress at C5 (salinity 0.7%) place
20.0% is reduced, and difference is extremely notable, germinating energy is progressively gently reduced without significant difference between C5～C7 process, under C8 process
Be greatly reduced, compared with CK reduce by 35.6%, as a result show, extra large rice 86 under salt stress its germinating energy in 0.7% salinity and 1.0%
Salinity has significant lower reduction；Extra large rice 86 can keep 93.3% germination percentage, its germination percentage to exist under 0.9% concentration
Just start to decline to a great extent under 1.0% concentration；Rise with NaCl solution concentration, height of seedling is decreased obviously, starts to show under 0.8% concentration
Write and decline；Extra large rice 86 shows extremely strong salt tolerance when salting liquid mass concentration is less than 0.1%, the salt tolerance more than 0.1%
Start to weaken, but still with stronger salt tolerance, the salt tolerance threshold range of extra large rice 86 is 0.9%～1.0%, is shown extremely strong
Salt tolerance.
As shown in Table 6, extra large rice 86 alkali salt stress under, with Na2CO3Solution concentration rises, and germinates under 0.3% concentration
Gesture remains to reach 88.9%, is significantly reducing higher than 0.30% concentration germinating energy, and in 0.35% concentration, germination percentage starts significantly
Reduce, start that germination is serious aggrieved in 0.4% concentration, i.e., extra large rice 86 can keep higher germinating energy in not higher than 0.30% concentration
And germination percentage；In 0.35% concentration, the extra large germination index of rice 86 starts to be remarkably decreased；In 0.15% concentration, height of seedling starts significantly
Reduce；Extra large rice 86 shows extremely strong salt tolerance when aqueous slkali mass concentration is less than 0.3%, and salt tolerance is opened more than 0.35%
Begin to weaken, the alkali resistance threshold range of extra large rice 86 is 0.30%～0.35%, shows extremely strong alkali resistance.
As shown in Table 7, all extremely strong Saline alkali tolerance is shown as in A1 (0.7%) process combinations, in A2 (0.8%) process
Combination starts to weaken, and alkali concn shows strong patience under 0.35% level, still, at A3 (0.9%) B2 (0.15%) place
Still strong Saline alkali tolerance, but alkali concn can be showed under reason combination no more than 0.15%, is combined in A4 (1.0%) process, it is resistance to
Saline alkali drastically weakens, or even in A4 (1.0%) B2 (0.15%), A4 (1.0%) B3 (0.25%), A4 (1.0%) B3
(0.25%) it is extremely weak to cash, and illustrates far to have broken through the extra large Saline alkali tolerance threshold value of rice 86 in these combinations.
Embodiment 2
1st, subjects：Huang Huazhan
2nd, test method：Processing method is same as Example 1, and difference is, the kind of yellow Hua Zhanwei not Salt And Alkali Tolerances,
It is not suitable for designing saline and alkaline cross matching.
3rd, result of the test and data processing：Germinating energy, germination percentage, germination index, seedlings of the Huang Huazhan in neutral salt solution
High, relatively resistance to salt damage rate, rank and salt tolerance power are shown in Table 8；Germinating energy, germinations of the Huang Huazhan in alkaline salt solution
Rate, germination index, height of seedling, relatively alkaline-resisting harmful rate, rank and alkali resistance power are shown in Table 9.
The yellow China of table 8 accounts for the result in neutral salt solution
The yellow China of table 9 accounts for the result in alkaline salt solution
As shown in Table 8, Huang Huazhan issues bud gesture and is just greatly reduced when 0.3% (C1) is processed in neutral salt stress, compared with CK
37.8% is reduced, NaCl solution concentration is improved, germinating energy is more and more lower, only 3.3% under 1.0% (C8) process, reduced compared with CK
92.3%；In 0.7% concentration, germination percentage starts to be greatly reduced；In 0.7% concentration, germination index starts to decline to a great extent；With
NaCl solution concentration rises, and height of seedling is decreased obviously, starts to be remarkably decreased in 0.3% concentration, the seedling height of seedling under 0.9% concentration
The about half of CK；Huang Huazhan shows extremely strong salt tolerance when salting liquid mass concentration is less than 0.6%, more than 0.7%
Salt tolerance is weaker, and the salt tolerance threshold range of Huang Huazhan is 0.6%～0.7%.
As shown in Table 9, Huang Huazhan under alkali salt stress, higher than 0.15% concentration opening by germinating energy, germination percentage and height of seedling
Beginning significantly reduces, and is the extremely sensitive alkali concn values of Huang Huazhan under 0.2%～0.25% concentration, germinates under 0.4% concentration and is subject to
It is serious to suppress；Huang Huazhan shows extremely strong salt tolerance when aqueous slkali mass concentration is less than 0.15%, resistance to more than 0.20%
Salt weakens, and the alkali resistance threshold range of Huang Huazhan is 0.15%～0.20%.
It is well known that the conventional not salt alkaline tolerance rice kinds of Huang Hua Zhanwei, extra large rice 86 is extremely strong Tolerant Cultivars, other paddy rice product
Plant can account for the examination obtained using the alkaline authentication method of Rice Salt of the present invention as positive negative control object with extra large rice 86 with Huang Hua
Test result to evaluate its Saline alkali tolerance trend.
The above, is only presently preferred embodiments of the present invention, not makes any restriction to the technical scope of the present invention, therefore
Any trickle amendment, equivalent variations and modification that every technical spirit according to the present invention is made to above example, still belong to
In the range of technical scheme.
Claims (6)
1. the authentication method of a kind of Rice Salt alkalescence, it is characterised in that the authentication method is comprised the following steps：
Step (1)：Prepare saline solution：A, prepare mass concentration gradient be 0.3%, 0.4%, 0.5%, 0.6%, 0.7%,
0.8%th, 0.9%, 1.0% NaCl salting liquids；B, prepare mass concentration gradient be 0.05%, 0.10%, 0.15%,
0.20%th, 0.25%, 0.30%, 0.35%, 0.40% Na2CO3Aqueous slkali；
Step (2)：Germination is processed：Some full rice paddy seeds are selected, fully 72～75h of immersion is straight in 25 DEG C of clear water
To showing money or valuables one carries unintentionally, then seed, to broken chest, is then uniformly placed in and is covered with the germination box of double-layer filter paper by vernalization under 30 DEG C of constant temperatures
In, the saline and alkaline concentration gradient solution prepared in step (1) is separately added into germination box immersion treatment as treatment group, with clear
Water immersion filter paper observes as a control group, daily humidity condition in germination box, with the uniform equivalent supplement process liquid of corresponding solution
To ensure humidity；
Step (3)：Observe and record germination result：Observation seed sprouting situation, that seed length is equal to the bud length of seed
Half, it is germination standard that root length is equal to seed length, investigates every 24h and records the germinative number of seed, and measures height of seedling, germination the 4th
It calculates germinating energy, calculates germination percentage within the 10th day；
Step (4)：As a result computing formula：
Germinating energy (%)=(the 4th day accumulative germination grain number/confession examination grain number) × 100；
Germination percentage (%)=(the 10th day accumulative germination grain number/confession examination grain number) × 100；
Germination index (GI)=∑ (Gt/Dt), wherein：Gt：T day germinative numbers, Dt：Germination number of days；
With respect to salt/alkali evil rate (%)=[(control group germination percentage-treatment group germination percentage)/treatment group germination percentage] × 100；
It is strong and weak to identify the Saline alkali tolerance of paddy rice by the way that result of calculation is compared with relative salt/alkali evil rate grade scale table.
2. the authentication method of Rice Salt as claimed in claim 1 alkalescence, it is characterised in that：In the step (1), with salt manufacturing
Alkali cross matching solution makees further identification, arranges neutral salinity and is：0.7%th, 0.8%, 0.9%, 1.0%, basic salt is dense
Degree 0.05%, 0.15%, 0.25%, 0.35% carries out combined crosswise as saline and alkaline cross matching solution.
3. the authentication method of Rice Salt as claimed in claim 1 alkalescence, it is characterised in that：The paddy rice is extra large rice 86 or Huang
China accounts for.
4. the authentication method of Rice Salt as claimed in claim 3 alkalescence, it is characterised in that：With extra large rice 86 and Huang Hua Zhanwei just
Negative Saline alkali tolerance is with reference to rice varieties.
5. the authentication method of Rice Salt as claimed in claim 3 alkalescence, it is characterised in that：The salt tolerance threshold value of the extra large rice 86
Scope is 0.9%～1.0%, and the alkali resistance threshold range of extra large rice 86 is 0.30%～0.35%；The salt tolerance threshold that the yellow China accounts for
Value scope is 0.15%～0.20% for the alkali resistance threshold range of 0.6%～0.7%, Huang Huazhan.
6. the authentication method of Rice Salt as claimed in claim 1 alkalescence, it is characterised in that：First will choose in the step (2)
The seed of choosing is sterilized with 3% potassium permanganate, clear water rinses 2～3 process.
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| CN113748787A (en) * | 2021-09-10 | 2021-12-07 | 海南浙江大学研究院 | Screening method of salt-tolerant or salt-sensitive rice seeds in germination period |
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| CN110583395A (en) * | 2019-09-11 | 2019-12-20 | 宁夏农林科学院农作物研究所（宁夏回族自治区农作物育种中心） | Method for evaluating salt tolerance of japonica rice seeds under salt stress |
| CN112273087A (en) * | 2020-10-30 | 2021-01-29 | 黑龙江八一农垦大学 | Method for identifying saline-alkali resistance of soybeans |
| CN113748787A (en) * | 2021-09-10 | 2021-12-07 | 海南浙江大学研究院 | Screening method of salt-tolerant or salt-sensitive rice seeds in germination period |
| CN113748787B (en) * | 2021-09-10 | 2022-08-16 | 海南浙江大学研究院 | Screening method of salt-tolerant or salt-sensitive rice seeds in germination period |
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