CN104206217A - Rice-variety salt-tolerance evaluating method - Google Patents
Rice-variety salt-tolerance evaluating method Download PDFInfo
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Abstract
The invention relates to a rice-variety salt-tolerance evaluating method and belongs to the technical field of plant breeding. The invention provides an identifying and evaluating method for a rice-variety salt-tolerance system, i.e., the salt tolerance of rice varieties is comprehensively evaluated under the same salt concentration and different salt concentrations at three salt sensitive periods, i.e. the budding and seedling period, the tillering stage and the booting stage of rice respectively at a laboratory, a manually-simulated salt pond and a saline soil field by adopting a total biomass-measuring method according to salt-tolerance indexes (the total biomass dry-weight specific-value percent of the rice varieties in a saline environment and a saltless environment). Compared with laboratory assessment in the prior art, the rice-variety salt-tolerance evaluating method has stronger practical effects and can be more directly applied to the planting of the rice varieties.
Description
Technical field
The present invention relates to field of plant variety breeding technology, particularly a kind of rice varieties salt endurance appraisement method.
Background technology
Saline land, whole world area accounts for land surface 1/3, accounts for 20% of world's total area under cultivation.China saline land area about 2,000 ten thousand hm2.Due to the impact of the factors such as mankind's activity, the salinization of soil just constantly engulfs the soil that the mankind depend on for existence, and the land area that can for cultivate reduces just increasingly.Paddy rice is the second largest cereal crops in the whole world, Annual planting area about 2,000,000,000 mu, and the people in the whole world nearly 1/3 take rice as staple food.Be first cereal crops in China paddy rice, China's Annual planting area about 500,000,000 be 1/3 of national grain acreage, and rice yield accounts for 44% of national total output of grain.From aspects such as China's water resource, weather and ecologies, except existing rice district, China also has some areas to be suitable for very much Rice Production, but fails due to the salination of soil to plant.Therefore cultivation salt tolerant rice kind or the salt resistance ability by cultivation step raising paddy rice are the availability and the economic benefit that improve saline and alkaline soil, alleviate one of preferred plan of world food crisis, significant.
Between rice varieties there is larger difference in salt resistance, carries out effective evaluation to salt resistance between rice varieties, is screening Rice Salt kind germ plasm resource, carries out the basis of salt tolerant rice rearing new variety.The research that salt tolerant rice kind is cultivated in the whole world starts from 1940.Within 1945, Sri Lanka once promoted salt tolerant rice kind Pokkali, but the time is not long.India is also the country comparatively early carrying out salt tolerant rice breeding.China is salt tolerant rice breeding research work (Tian Shaohua and brilliant luxuriant, 1987) from the seventies in last century.Zhao Shouren etc. (1985) cooperate with International Rice Research Institute, the excellent salt tolerant rice kind 80 ~ 85 that Screening and Identification obtains in more than 500 parts of salt tolerant rice materials, and this kind high resistance is saline and alkaline, stooling is strong, fertilizer resistance anti-lodging, high yield characteristic is better, applies area also little.Fang Xianwen etc. (2004) 0.8%NaCI solution and International Rice institute Salt Resistance of Rice 9 grades of evaluation methods (Banos, 2002) screening obtain extremely salt-tolerant rice varieties 6 parts in seedling stage.The research work great majority of above Selection of Salt-Tolerant rice varieties carry out selection of salt tolerance to the sizing rice varieties be bred as, have accumulated more selection of salt tolerance method and standard simultaneously, as Their Seed Germinating Period qualification, seedling stage assay, field growing middle and later periods Screening and Identification etc., achieve certain progress, the good rice varieties of salt resistance as screening has 80-85, Yancheng 156, salt rich 47 etc., these salt tolerant rice variety popularization application areas are all little, at present all also as the also application in the researchs such as breeding of salt tolerant resource.Generally speaking salt tolerant rice breed breeding progress is slow, cannot meet the demand of saline land rice cultivation to salt tolerant rice kind.Tracing it to its cause, is lack one to the effective evaluation method of rice varieties salt resistance, thus makes Rice Salt kind Screening of Germplasm and cultivate salt tolerant rice new varieties to bring serious technology barrier.
At present, to rice varieties Evaluation of Salt Tolerance, mostly limit to and in laboratory environments the paddy rice budding period is carried out, not having can in the particularly salt density value phase in the time of infertility of paddy rice, carry out Salt Resistance of Rice evaluation, because laboratory environment and saline land field conditions exist very large gap, the result causing the result of laboratory qualification and field to be planted on the spot is often very inconsistent, lacks general systematicness and actual effect.
Summary of the invention
For solving above-mentioned prior art Problems existing, the object of the present invention is to provide a kind of rice varieties salt endurance appraisement method, under identical salinity and different salinity, rudiment in paddy rice becomes seedling stage, tillering stage, three salt density value phases of booting stage, respectively in laboratory, manual simulation salt pond and solonchak are on the spot, adopt " full biomass estimation method ", with salt tolerance index (rice varieties under salt environment with without biomass ratio percentage complete under salt environment), salt resistance is carried out to rice varieties and carry out thoroughly evaluating, the result of qualification is consistent with the result that field is planted on the spot, the guidance of rice varieties salt resistance can be applied to.
For achieving the above object, technical scheme of the present invention is:
To a System Assessment Method for rice varieties salt resistance, comprise the following steps:
Step one: in laboratory environments, under same salinity (0.5%), rice varieties seed is commented to carry out rudiment seed-forming experiment to all confessions, wholeheartedly become to carry out seedling stage full biomass dry matter to three leaves to measure, and with carry out one_to_one corresponding without the full biomass dry matter of control group same breed paddy rice under salt environment and compare, calculate the salt tolerance index of each experimental cultivar respectively, described salt tolerance index both under salt environment with without biomass dry matter ratio percentage complete under salt environment, the kind being greater than 50% using clear and definite salt tolerance index proceeds the experimental cultivar of Evaluation of Salt Tolerance as next step,
Step 2: carry out in manual simulation salt pond, under different salinity, arrange 0.2%, 0.3%, 0.4%, 0.5%, 0.8% 5 salinity degree of passing, as supplying, kind is commented to the kind being greater than 50% by salt tolerance index in step one laboratory rudiment seed-forming experiment, carry out rice plant of tillering stage Evaluation of Salt Tolerance test, to nine leaves wholeheartedly tillering regularity carry out full biomass dry matter measure, and with carry out without the control group same breed paddy rice under salt environment that full biomass dry matter is corresponding to be compared, calculate the salt tolerance index of each experimental cultivar respectively, evaluate rice varieties with this to tiller Adult plant salt resistance and salt tolerant degree thereof,
Step 3: carry out on the spot at Coastal beach solonchak, under different salinity, select 0.2%, 0.3%, 0.4%, 0.5%, the solonchak of 0.8% 5 salinity degree of passing on the spot, as supplying, kind is commented to the kind being greater than 50% by salt tolerance index in step one laboratory rudiment seed-forming experiment, carry out the test of Rise's boot period Evaluation of Salt Tolerance, in paddy rice booting--carry out full biomass dry matter heading stage and measure, and with carry out without the control group same breed paddy rice under salt environment that full biomass dry matter is corresponding to be compared, calculate the salt tolerance index of each experimental cultivar respectively, evaluate rice varieties with this to tiller Adult plant salt resistance, rice varieties booting salt resistance at heading stage and salt tolerant degree thereof is evaluated with this,
Step 4: carry out total score bleed rice varieties in Seedling Stage, Adult plant of tillering, the salt resistance of three salt density value phases of booting stage to above-mentioned steps one, two, three test for identification result, determines that salt resistance and the salt tolerant degree thereof of rice varieties are commented in each confession.
Salt tolerance index (salt tolerance index): be the index evaluating rice varieties salt resistance power, by the up-to-date proposition of the present invention, the percentage of the full biomass dry matter G2 ratio grown under the full biomass dry matter G1 grown under salt environment with rice varieties and non-salt environment, represent with N, N=G1/G2 × 100%.The size of salt tolerance index reflects that a certain rice varieties restrains oneself the power of ability to salt stress.
Relative to prior art, beneficial effect of the present invention is: the present invention proposes to adopt " full biomass estimation method " first, with salt tolerance index, salt resistance is carried out to rice varieties and carry out thoroughly evaluating, measuring salt tolerance index is that metrics evaluation rice varieties salt resistance is strong and weak, being the rice varieties salt endurance appraisement method of a kind of " easy, practical, science, novelty ", providing technical support for carrying out salt tolerant rice correlative study.
Embodiment
Below in conjunction with embodiment, the present invention program is described in further detail,
To a System Assessment Method for rice varieties salt resistance, comprise the following steps:
The rudiment of first step laboratory becomes Salt-Tolerance Identification in seedling stage
Salinity 0.5% time, rice varieties seed is commented to carry out rudiment seedling to supplying, carry out full biomass dry matter to three leaf one heart stages to measure, and with contrast (without salt environment) full biomass dry matter carry out that same kind is corresponding to be compared, measure the salt tolerance index (under salt environment with without biomass dry matter ratio complete under salt environment) of each experimental cultivar, the kind being greater than 50% using clear and definite salt tolerance index proceeds the experimental cultivar of Evaluation of Salt Tolerance as next step.
Second step manual simulation salt pond Salt-Tolerance Identification in tillering stage
0.2%, 0.3%, 0.4%, 0.5%, 0.8% 5 salinity degree of passing is set in manual simulation salt pond, as supplying, kind is commented to the kind being greater than 50% by salt tolerance index in previous step laboratory rudiment seed-forming experiment, plant, Yu Jiuye wholeheartedly tillering regularity carries out full biomass dry matter mensuration, calculate the salt tolerance index of each experimental cultivar, evaluate rice varieties with this and to tiller Adult plant salt resistance and salt tolerant degree thereof.
3rd step coastal saline soil on the spot booting stage Salt-Tolerance Identification
The solonchak of 0.2%, 0.3%, 0.4%, 0.5%, 0.8% 5 salinity degree of passing is selected on the spot at Coastal beach, rice varieties is commented to carry out Evaluation of Salt Tolerance test in booting stage to supplying, in paddy rice booting--carry out full biomass dry matter heading stage and measure, calculate the salt tolerance index of each experimental cultivar.Evaluate rice varieties with this to tiller Adult plant salt resistance, evaluate rice varieties booting salt resistance at heading stage and salt tolerant degree thereof with this.
4th step comments rice varieties to carry out salt resistance overall merit to supplying
Above-mentioned first, second, third step test for identification result is comprehensively analyzed, determines that salt resistance and the salt tolerant degree thereof of rice varieties are commented in each confession.
The above, be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any change of expecting without creative work or replacement, all should be encompassed within protection scope of the present invention.Therefore, the protection domain that protection scope of the present invention should limit with claims is as the criterion.
Claims (4)
1. a System Assessment Method for rice varieties salt resistance, is characterized in that, comprises the following steps:
Step one: in laboratory environments, under same salinity, rice varieties seed is commented to carry out rudiment seed-forming experiment to all confessions, wholeheartedly become to carry out seedling stage full biomass dry matter to three leaves to measure, and with carry out one_to_one corresponding without the full biomass dry matter of control group same breed paddy rice under salt environment and compare, calculate the salt tolerance index of each experimental cultivar respectively, described salt tolerance index both under salt environment with without biomass dry matter ratio percentage complete under salt environment, the kind being greater than 50% using clear and definite salt tolerance index proceeds the experimental cultivar of Evaluation of Salt Tolerance as next step;
Step 2: carry out in manual simulation salt pond, under different salinity, different salinity degree of passing is set, as supplying, kind is commented to the kind being greater than 50% by salt tolerance index in step one laboratory rudiment seed-forming experiment, carry out rice plant of tillering stage Evaluation of Salt Tolerance test, to nine leaves wholeheartedly tillering regularity carry out full biomass dry matter measure, and with carry out without the control group same breed paddy rice under salt environment that full biomass dry matter is corresponding to be compared, calculate the salt tolerance index of each experimental cultivar respectively, evaluate rice varieties with this and to tiller Adult plant salt resistance and salt tolerant degree thereof;
Step 3: carry out on the spot at Coastal beach solonchak, under different salinity, select the solonchak of different salinity degree of passing on the spot, as supplying, kind is commented to the kind being greater than 50% by salt tolerance index in step one laboratory rudiment seed-forming experiment, carry out the test of Rise's boot period Evaluation of Salt Tolerance, in paddy rice booting--carry out full biomass dry matter heading stage and measure, and with carry out without the control group same breed paddy rice under salt environment that full biomass dry matter is corresponding to be compared, calculate the salt tolerance index of each experimental cultivar respectively, evaluate rice varieties with this to tiller Adult plant salt resistance, rice varieties booting salt resistance at heading stage and salt tolerant degree thereof is evaluated with this,
Step 4: carry out total score bleed rice varieties in Seedling Stage, Adult plant of tillering, the salt resistance of three salt density value phases of booting stage to above-mentioned steps one, two, three test for identification result, determines that salt resistance and the salt tolerant degree thereof of rice varieties are commented in each confession.
2. the System Assessment Method of rice varieties salt resistance according to claim 1, is characterized in that, in described step one, salinity chooses 0.5%.
3. the System Assessment Method of rice varieties salt resistance according to claim 1, is characterized in that, in described step 2, is chosen for 0.2%, 0.3%, 0.4%, 0.5%, 0.8% 5 different salinity degree of passing and carries out the experiment of manual simulation salt pond.
4. the System Assessment Method of rice varieties salt resistance according to claim 1, is characterized in that, in described step 3, select salt concentration gradient be 0.2%, 0.3%, 0.4%, 0.5%, 0.8% the Coastal beach solonchak that carries out on the spot test on the spot.
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Cited By (10)
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| CN104604554A (en) * | 2015-01-13 | 2015-05-13 | 江苏省农业科学院 | Salt-tolerant sorgo germplasm identification method |
| CN105265206A (en) * | 2015-11-16 | 2016-01-27 | 江苏省农业科学院 | Method for testing salt tolerance of greening nursery stock in saline land |
| CN106416864A (en) * | 2016-09-30 | 2017-02-22 | 江苏丘陵地区镇江农业科学研究所 | Method for quickly identifying salt tolerance of rice at seedling stages |
| CN106612737A (en) * | 2016-12-17 | 2017-05-10 | 长江大学 | Verification method for saline-alkaline resistance of paddy rice |
| CN108513882A (en) * | 2018-04-08 | 2018-09-11 | 新疆维吾尔自治区环境保护科学研究院 | A kind of method of field herbaceous plant environmental Kuznets Curves experiment |
| CN109076905A (en) * | 2018-07-23 | 2018-12-25 | 深圳威琳懋生物科技有限公司 | A kind of high quality white rice seed screening technique |
| CN111492919A (en) * | 2020-04-16 | 2020-08-07 | 江苏沿海地区农业科学研究所 | High-salt-tolerance rice germplasm identification and screening method |
| CN113748787A (en) * | 2021-09-10 | 2021-12-07 | 海南浙江大学研究院 | Screening method of salt-tolerant or salt-sensitive rice seeds in germination period |
| CN114128619A (en) * | 2021-10-14 | 2022-03-04 | 江苏沿海地区农业科学研究所 | Method for rapidly breeding salt-tolerant rice in whole growth period |
| CN114731799A (en) * | 2022-05-12 | 2022-07-12 | 新疆农业科学院温宿水稻试验站 | Rapid screening method of saline-alkali-resistant rice breeding material |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104604554A (en) * | 2015-01-13 | 2015-05-13 | 江苏省农业科学院 | Salt-tolerant sorgo germplasm identification method |
| CN104604554B (en) * | 2015-01-13 | 2017-02-01 | 江苏省农业科学院 | Salt-tolerant sorgo germplasm identification method |
| CN105265206A (en) * | 2015-11-16 | 2016-01-27 | 江苏省农业科学院 | Method for testing salt tolerance of greening nursery stock in saline land |
| CN106416864A (en) * | 2016-09-30 | 2017-02-22 | 江苏丘陵地区镇江农业科学研究所 | Method for quickly identifying salt tolerance of rice at seedling stages |
| CN106612737A (en) * | 2016-12-17 | 2017-05-10 | 长江大学 | Verification method for saline-alkaline resistance of paddy rice |
| CN108513882A (en) * | 2018-04-08 | 2018-09-11 | 新疆维吾尔自治区环境保护科学研究院 | A kind of method of field herbaceous plant environmental Kuznets Curves experiment |
| CN109076905A (en) * | 2018-07-23 | 2018-12-25 | 深圳威琳懋生物科技有限公司 | A kind of high quality white rice seed screening technique |
| CN111492919A (en) * | 2020-04-16 | 2020-08-07 | 江苏沿海地区农业科学研究所 | High-salt-tolerance rice germplasm identification and screening method |
| CN113748787A (en) * | 2021-09-10 | 2021-12-07 | 海南浙江大学研究院 | Screening method of salt-tolerant or salt-sensitive rice seeds in germination period |
| CN113748787B (en) * | 2021-09-10 | 2022-08-16 | 海南浙江大学研究院 | Screening method of salt-tolerant or salt-sensitive rice seeds in germination period |
| CN114128619A (en) * | 2021-10-14 | 2022-03-04 | 江苏沿海地区农业科学研究所 | Method for rapidly breeding salt-tolerant rice in whole growth period |
| CN114731799A (en) * | 2022-05-12 | 2022-07-12 | 新疆农业科学院温宿水稻试验站 | Rapid screening method of saline-alkali-resistant rice breeding material |
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