CN106591132A - Rapid and efficient chlorella collection method - Google Patents
Rapid and efficient chlorella collection method Download PDFInfo
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- CN106591132A CN106591132A CN201510686359.3A CN201510686359A CN106591132A CN 106591132 A CN106591132 A CN 106591132A CN 201510686359 A CN201510686359 A CN 201510686359A CN 106591132 A CN106591132 A CN 106591132A
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- chlorella
- chitosan
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Abstract
The present invention relates to the field of aquaculture, particularly to a rapid and efficient chlorella collection method, wherein a proper amount of chitosan is added to a chlorella liquid to achieve the flocculation and sedimentation effect. Compared to the method in the prior art, the method of the present invention has the following characteristics that chitosan is used to collect chlorella, such that the flocculation efficiency is high, the consumption is low, the cost is saved, the operation is convenient, and the method is simple. According to the present invention, chitosan is non-toxic, tasteless and biodegradable, does not cause secondary pollution, is the ideal flocculant for chlorella collection, and can be widely promoted in the field of microalgae industry.
Description
Technical field
The present invention relates to technical field of aquaculture, and in particular to a kind of method for rapidly and efficiently harvesting chlorella.
Background technology
Chlorella (Chlorella) is a kind of common monoplast green alga, belongs to common ball algae guiding principle, is a kind of efficient photosynthetic
Plant, is also microalgae that the first is manually cultivated in being widely distributed in fresh water and seawater.Chlorella contains abundant egg
The nutrients such as white matter, amino acid, polysaccharide, vitamin, unrighted acid, mineral matter, growth factor and cytochromes
Matter, with very abundant nutrient content and excellent medical care effect in a balanced way.In China, although chlorella has been thrown
Enter large-scale production, but its harvesting cost still accounts for more than the 30% of totle drilling cost, is unfavorable for the popularization of industrialization.
The harvesting of China's microalgae at present mainly has two kinds of Physical and chemical method.Physical is relatively more to instrument and equipment requirement,
Harvesting high cost, Chemical picking method mainly uses flocculant flocculation sediment microalgae, and process is simply easily operated, but wadding
The safety problems such as the residual and toxicity of solidifying agent, often limit its application in food industry.
Shitosan is a kind of natural high polymer coagulant, nontoxic pollution-free and with excellent histocompatbility, degradable
Property, thus as a kind of excellent bioabsorbable polymer material be widely used in chemistry, food, weaving, environmental protection etc. each
Field.
By the use of shitosan as flocculant microalgae, existing a small amount of relevant report domestic at present, but its technology is immature,
Cumbersome high cost, it is impossible to use in a large number for a long time.
The content of the invention
To solve prior art problems, we provide a kind of method for rapidly and efficiently harvesting chlorella.
The present invention concrete technical scheme be：
Step one prepares chitosan solution；
Step 2 arranges different experiments group, by flocculating effect preliminary experiment of the variable concentrations shitosan to chlorella, it is determined that most
Suitable flocculation chitosan concentration；
Step 3 under agitation, chitosan solution is slowly added dropwise in bead ball algae solution, is stood after stirring 3min；
Step 4 treats that the basic flocculating setting of chlorella is finished, and siphons away supernatant, you can obtain concentration algae solution.
Preferably, in step one, with preparing again after 1% acetum dissolving, compound concentration is 1% to shitosan, is now used
Now match somebody with somebody.
Preferably, in step 3, time of repose is less than 1h.
The invention has the advantages that：
Compared with original technology, chlorella is harvested with shitosan, flocculation efficiency is high and consumption is few, not only saves cost,
And easy to operate method is simple.Shitosan is nonpoisonous and tasteless, biodegradable not result in secondary pollution, is harvesting bead
The preferable flocculant of algae, can be widely popularized in microalgae industry field.
Specific embodiment
With reference to specific embodiment, the present invention will be described in detail：
The embodiment is preferred version, is mainly included the following steps that：
Step one prepares chitosan solution；
Step 2 arranges different experiments group, by flocculating effect preliminary experiment of the variable concentrations shitosan to chlorella, it is determined that most
Suitable flocculation chitosan concentration is 0.05g/L；
Step 3 under agitation, chitosan solution is slowly added dropwise in bead ball algae solution, is stood after stirring 3min；
The chlorella in 30min finishes step 4 with regard to the basic flocculating setting of energy, and flocculating rate siphons away supernatant up to more than 90%
Liquid, you can obtain concentration algae solution.
Preferably, in step one, with preparing again after 1% acetum dissolving, compound concentration is 1% to shitosan, is now used
Now match somebody with somebody.
Above are only a specific embodiment of the present invention, it is clear that the invention is not restricted to above example, can also have
Many deformations.All deformations that one of ordinary skill in the art can directly derive from present disclosure or associate,
It is considered as protection scope of the present invention.
Claims (3)
1. it is a kind of rapidly and efficiently harvest chlorella method, it is characterised in that comprise the following steps：
Step one prepares chitosan solution；
Step 2 arranges different experiments group, by flocculating effect preliminary experiment of the variable concentrations shitosan to chlorella, it is determined that most
Suitable flocculation chitosan concentration；
Step 3 under agitation, chitosan solution is slowly added dropwise in bead ball algae solution, is stood after stirring 3min；
Step 4 treats that the basic flocculating setting of chlorella is finished, and siphons away supernatant, you can obtain concentration algae solution.
2. the method for rapidly and efficiently harvesting chlorella according to claim 1, it is characterised in that in step one, shell
Glycan is prepared again after being dissolved with 1% acetum, chitosan solution matching while using.
3. the method for rapidly and efficiently harvesting chlorella according to claim 1, it is characterised in that quiet in step 3
The time is put less than 1h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510686359.3A CN106591132A (en) | 2015-10-20 | 2015-10-20 | Rapid and efficient chlorella collection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510686359.3A CN106591132A (en) | 2015-10-20 | 2015-10-20 | Rapid and efficient chlorella collection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106591132A true CN106591132A (en) | 2017-04-26 |
Family
ID=58555232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510686359.3A Pending CN106591132A (en) | 2015-10-20 | 2015-10-20 | Rapid and efficient chlorella collection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106591132A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384720A (en) * | 2018-05-03 | 2018-08-10 | 江西理工大学 | A method of it is flocculated using combination flocculant and harvests chlorella pyrenoidosa |
| CN109430651A (en) * | 2018-11-26 | 2019-03-08 | 江苏科技大学 | A kind of chlorella health beverages and preparation method thereof |
-
2015
- 2015-10-20 CN CN201510686359.3A patent/CN106591132A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384720A (en) * | 2018-05-03 | 2018-08-10 | 江西理工大学 | A method of it is flocculated using combination flocculant and harvests chlorella pyrenoidosa |
| CN109430651A (en) * | 2018-11-26 | 2019-03-08 | 江苏科技大学 | A kind of chlorella health beverages and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170426 |
|
| WD01 | Invention patent application deemed withdrawn after publication |