CN106587342A - Bioremediation reagent and application thereof in removing 1,2-dichloroethane in composite polluted water body - Google Patents
Bioremediation reagent and application thereof in removing 1,2-dichloroethane in composite polluted water body Download PDFInfo
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- CN106587342A CN106587342A CN201611244353.1A CN201611244353A CN106587342A CN 106587342 A CN106587342 A CN 106587342A CN 201611244353 A CN201611244353 A CN 201611244353A CN 106587342 A CN106587342 A CN 106587342A
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- dichloroethanes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a bioremediation reagent for removing 1,2-dichloroethane in a composite polluted underground water. The bioremediation reagent comprises sodium lactate, vitamin B12, monopotassium phosphate, disodium hydrogen phosphate and distilled water. The bioremediation reagent is simple in component, low in treatment cost and remarkable in treatment effect, the removal rate of 1,2-dichloroethane (50mg/L) can be up to 96% and greater within two weeks, and 1,2-dichloroethane can be completely removed within one month.
Description
Technical field
It is the present invention relates to technical field of sewage more particularly to a kind of using can be used to remove in combined pollution subsoil water
The biological restoration reagent of 1,2- dichloroethanes
Background technology
1,2- dichloroethanes (1,2-Dichloroethane, referred to as 1,2-DCA) are a kind of volatile hypertoxic chlorohydrocarbons
Organic substance, is one of most strong pollutant of aquifer underground water containing chlorine pollutant toxicity.Its annual production more than 5.443 ×
109Kg, is most industrial halides of yield in the world, and it has of a relatively high water solublity (8524mg/L) and in soil
Potential mobility.Due to widely using and incorrect disposal, what 1,2- dichloroethanes had become in 0 groundwater environment common has
Organic pollutants：Environmental Protection Agency USA determines 1,2- dichloroethanes for one of priority pollutants；Gao Cunrong etc. is studied and pointed out,
In 38 organic contamination indexs in 69 urban groundwaters of China, 1,2- dichloroethanes are main volatile organic contaminants
One of.1,2- dichloroethanes density ratio water is big, once will be with dense non-aqueous phase liquids (dense non-into subsoil water
Aqueous phase liquid, DNAPL) form presence, often movement is slow for subsoil water Jing so that the residual of 1,2- dichloroethanes
Time may continue several years to upper a century, it is thus possible to cause lasting environment and health risk.
Restorative procedure disclosed in prior art about 1,2- dichloroethanes in subsoil water mainly has physical method, chemistry side
Method and biological method etc..Wherein physical method includes underground water aeration technology and soil gas extraction technique, and chemical method includes
Oxidizing method, electrochemical oxidation process and nanometer reagent reducing process, although above two method can quickly remove 1,2- bis-
Ethyl chloride, but Expenses Cost is higher, administers not thorough；And using traditional chemical reduction means, such as Zero-valent Iron and nanometer
Ferrum reduction technique can not remove 1,2- dichloroethanes, with Nanometer Copper as catalyst, can be also by electron donor of sodium borohydride
Former 1,2-DCA, but reduce not thoroughly, the bigger by-product of the toxicity such as a vinyl chloride can be produced, while there is also due to adding medicine
The risk of the secondary pollution that agent brings.
The content of the invention
In view of this, it is an object of the invention to provide a kind of biological restoration reagent, the biological restoration reagent can activate original
The activity of dechlorination functional microorganism so as to remove combined pollution water body in 1,2- dichloroethanes, it is possible to achieve it is innoxious, without two
Secondary pollution, and processing cost is cheap.
In order to realize foregoing invention purpose, the present invention provides technical scheme below：
The invention provides a kind of biological restoration reagent, with water as solvent, the component comprising following content：
5～40g/L of sodium lactate；
Vitamin B120.1g～5g/L；
KH2PO41.08～7.95g/L；
Na2HPO412.99～20.17g/L.
The invention provides a kind of biological restoration reagent, with water as solvent, the component comprising following content：
10～20g/L of sodium lactate；
Vitamin B120.1～0.5g/L；
KH2PO43.88～5.71g/L；
Na2HPO415.36～17.25g/L.
The invention provides application of the above-mentioned biological restoration reagent in combined pollution water body is removed in 1,2- dichloroethanes.
Following pollutant are included in the combined pollution water body：Trichloro ethylene, chloroform and 1,2- dichloroethanes.
Pollutant comprising following concentration in the combined pollution water body：
Trichloro ethylene 40mg/L
Chloroform 50mg/L
1,2- dichloroethanes 50mg/L
The biological restoration reagent is 1 with the volume ratio of polluted-water：100～200.
Technique effect：Because the groundwater environment of 1,2- dichloroethanes pollution is mostly anaerobic environment, Jing often lacks effective
Reduction dechlorination function bacterium or the activity of reduction dechlorination function bacterium be suppressed, so as to cause pollution environment in situ in selfreparing
Cycle is very long, or even lacks biological self-reparing capability；Simultaneously because the chloroethenes of major pollutants 1,2- bis- in underground water pollution environment
Alkane Jing often forms combined pollution with other halogenated hydrocarbons, and this has just been further exacerbated by major pollutants 1, and the removal of 2- dichloroethanes is difficult
Degree.Biological restoration reagent of the present invention can effectively activate the activity of reduction dechlorination functional microorganism in pollution environment in situ,
And be allowed to play the 1,2- dichloroethanes that dechlorination function is removed in combined pollution water body in combined pollution environment.
The biological restoration reagent that the present invention is provided is with strong points, provides most suitable for the dechlorination functional microorganism in situ environment
Carbon source, the required cofactor of dechlorination enzyme and most suitable dechlorination acid or alkali environment；Removal effect is obvious, non-secondary pollution,
The reagent can completely remove 1, the 2- dichloroethanes in combined pollution subsoil water, and end-product is nontoxic ethylene；Process cycle compared with
It is short, 1, the 2- dichloroethanes of 50mg/L can be removed in one month complete；The agent formulations (place simple and easy to get and cheap
The reagent cost of 1 ton of 1,2- dichloroethanes polluted underground water containing 50mg/L of reason is less than 10 yuan).
Specific embodiment
The invention provides a kind of biological restoration reagent, with water as solvent, the component comprising following content：
5～40g/L of sodium lactate；
Vitamin B120.1g～5g/L；
KH2PO41.08～7.95g/L；
Na2HPO412.99～20.17g/L.
In the present invention, the mass fraction of the sodium lactate is preferably 10～20g/L, more preferably 10g/L；The dimension life
Plain B12Mass fraction be preferably 0.1～0.5g/L, more preferably 0.1g/L；The KH2PO4Preferably 3.88～5.71g/L,
More preferably 3.88g/L；The Na2HPO4Preferably 15.36～17.25g/L, more preferably 17.25g/L.
The present invention is preferably by sodium lactate, vitamin B12、KH2PO4And Na2HPO4Each raw material is configured in the distilled water being dissolved in
Mass fraction be above-mentioned mass fraction biological restoration reagent.
The invention provides application of the above-mentioned biological restoration reagent in combined pollution water body is removed in 1,2- dichloroethanes.
Preferably comprise following pollutant in the combined pollution water body in the present invention：Trichloro ethylene, chloroform and 1,2- bis-
Ethyl chloride.
Combined pollution water body of the present invention is preferably chemical industry for making chlorine and alkali factory because of leakage or the subsoil water of exhaust emission, organochlorine
For the waste water of hydrocarbon processing maker, the polluted underground water that Oil spills is caused.
In the present invention the concentration of the trichloro ethylene is preferably 0～60mg/L, more preferably 40mg/L；The chloroform
Concentration is preferably 0～60mg/L, more preferably 50mg/L；The concentration of 1, the 2- dichloroethanes is preferably 0～100mg/L, more
Preferably 50mg/L.
The present invention is processed after preferably the biological restoration reagent for obtaining is mixed with polluted-water, described in the present invention
Biological restoration reagent is preferably 1 with the volume ratio of polluted-water：100～200, more preferably 1：100～150.During the process
Between be preferably 10～30 days, more preferably 10～20 days.
What the process of heretofore described sewage disposal was preferably carried out under anaerobism or anoxia condition, build in the present invention
Preferably pre- deoxygenation is carried out with nitrogen during simulated system, reaction system is placed in closed anaerobism serum bottle.
With reference to embodiment to the present invention provide biological restoration reagent and its remove combined pollution water body in 1,2-
Application in dichloroethanes is described in detail, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
Original position impurely descends the construction step of combined pollution water model system as follows：
(1) N is used2Pre- purging, 15min are carried out to serum bottle (volume is 100mL)；
(2) the polluted underground water in situ injection for drawing 40mL with syringe is washed in the serum bottle blown over, and beyond the Great Wall plug, uses aluminum
Lid is compressed；
(3) chloroform (the about 50mg/ of 1uL trichloro ethylenes (about 40mg/L), 1.5uL is added toward system with microsyringe
L) and 1,2- dichloroethanes (about 50mg/L), for simulating the distribution situation of polluted underground water major pollutants in situ.
Removal of the biological restoration reagent of the present invention to 1,2- dichloroethanes in above-mentioned polluted underground water simulated system in situ：
(1) add with water as solvent in above-mentioned simulated system, the biological restoration reagent comprising following weight/mass percentage composition：
Sodium lactate 10g/L；
Vitamin B120.1g/L；
KH2PO43.88g/L；
Na2HPO4 17.25g/L
The biological restoration reagent is 1 with the volume ratio of combined pollution subsoil water：100.
(2) to be not added with the blank system of biological restoration reagent as control, two per group parallel, averages as detection
As a result；
(3) with headspace gas detection method (GC-FID) detect biological restoration reagent in 15 days and 30 days to 1,2- dichloros
Ethane clearance, (as shown in table 1).
The biological restoration reagent of table 1 is to 1,2- dichloroethanes removal effects
| Polluted underground water simulated system | 15d clearances | 30d clearances |
| Blank | 0.00% | 0.00% |
| Biological restoration reagent | 94.39% | 100% |
As a result show：Biological restoration reagent of the present invention has good removal effect to 1,2- dichloroethanes, in two weeks
Clearance reach 94.39%, 1, the 2- dichloroethanes of about 50mg/L can be completely removed in one month.
Embodiment 2
(1) construction step of In-situ reaction polluted underground water simulated system is same as Example 1.
(2) biological restoration reagent, the biological restoration reagent and combined pollution subsoil water are added in above-mentioned simulated system
Volume ratio be 1：100, in the biological restoration reagent concentration of sodium lactate be respectively 80g/L, 40g/L, 20g/L, 10g/L,
5g/L、2.5g/L；Replace the blank system of biological restoration reagent as control using the distilled water of equal volume, two per group are put down
OK, average as testing result；
(3) with headspace gas detection method (GC-FID) detection is different biological restoration reagent is organized in 10 days and 20 days to 1,2-
Dichloroethanes clearance, (as shown in table 2).
Removal effect of the biological restoration reagent of the different carbon source addition of table 2 to 1,2- dichloroethanes
| Polluted underground water simulated system | 10d clearances | 20d clearances |
| Blank | 0.00% | 0.00% |
| 80g/L carbon source biological restoration reagents | 11.60% | 13.66% |
| 40g/L carbon source biological restoration reagents | 45.17% | 91.02% |
| 20g/L carbon source biological restoration reagents | 97.27% | 96.64% |
| 10g/L carbon source biological restoration reagents | 96.53% | 96.42% |
| 5g/L carbon source biological restoration reagents | 86.58% | 96.54% |
| 2.5g/L carbon source biological restoration reagents | 52.50% | 60.82% |
As a result show：When the addition concentration of sodium lactate has preferably in the range of 5～20mg/L to 1,2- dichloroethanes
Removal effect (clearance reaches more than 95%), considers removal cycle and clearance factor, final to determine that 10g/L is lactic acid
The optimal input concentration of sodium
Comparative example 1
Compare removal effect of the different carbon source biological restoration reagent to 1,2- dichloroethanes
(1) change and repair carbon source composition in reagent, the sodium lactate in embodiment 2 is replaced with into sodium acetate, sodium benzoate, Mus
Lee's glycolipid, starch, Oleic acid, glycerol, Oleum Glyciness so that the mean carbon content of carbon source is consistent with the sodium lactate phosphorus content of 10g/L, with
The distilled water of equal volume replaces the blank system of biological restoration reagent as control, two per group parallel, conducts of averaging
Testing result；
(2) with headspace gas detection method (GC-FID) detection is different biological restoration reagent is organized in 15 days to the chloroethenes of 1,2- bis-
Alkane clearance；(as shown in table 3)
Removal effect of the different carbon source biological restoration reagent of table 3 to 1,2- dichloroethanes
| Polluted underground water simulated system | Clearance |
| Subsoil water blank | 0.00% |
| Sodium lactate biological restoration reagent | 85.56% |
| Oleic acid biological restoration reagent | 0.00% |
| Sodium acetate biological restoration reagent | 0.00% |
| Sodium benzoate biological restoration reagent | 0.00% |
| Oleum Glyciness biological restoration reagent | 0.00% |
| Glycerol biological restoration reagent | 0.00% |
| Rhamnolipid biological repairs reagent | 0.44% |
| Starch biological restoration reagent | 13.71% |
As a result show：In the repairing efficiency of two weeks, sodium lactate biological restoration reagent to 1 in combined pollution subsoil water,
2- dichloroethanes have highest remediation efficiency.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of biological restoration reagent, it is characterised in that with water as solvent, comprising following components：
5～40g/L of sodium lactate；
Vitamin B120.1g～5g/L；
KH2PO41.08～7.95g/L；
Na2HPO412.99～20.17g/L.
2. reparation reagent according to claim 1, it is characterised in that with water as solvent, the component comprising following content：
10～20g/L of sodium lactate；
Vitamin B120.1～0.5g/L；
KH2PO43.88～5.71g/L；
Na2HPO415.36～17.25g/L.
3. the biological restoration reagent described in claim 1～2 any one remove combined pollution water body in 1,2- dichloroethanes
In application.
4. application according to claim 3, it is characterised in that following pollutant are included in the combined pollution water body：Three
Vinyl chloride, chloroform and 1,2- dichloroethanes.
5. application according to claim 4, it is characterised in that state the pollution comprising following concentration in combined pollution water body
Thing：
Trichloro ethylene 40mg/L
Chloroform 50mg/L
1,2- dichloroethanes 50mg/L.
6. application according to claim 3, it is characterised in that the biological restoration reagent is with the volume ratio of polluted-water
1：100.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611244353.1A CN106587342A (en) | 2016-12-29 | 2016-12-29 | Bioremediation reagent and application thereof in removing 1,2-dichloroethane in composite polluted water body |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611244353.1A CN106587342A (en) | 2016-12-29 | 2016-12-29 | Bioremediation reagent and application thereof in removing 1,2-dichloroethane in composite polluted water body |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109019819A (en) * | 2018-09-04 | 2018-12-18 | 上海格林曼环境技术有限公司 | For the reduction medicament of chlorohydrocarbon polluted underground water in-situ immobilization and its preparation and application method |
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| CN102815794A (en) * | 2012-08-28 | 2012-12-12 | 环境保护部华南环境科学研究所 | Composite bio-energizer used for in-situ ecological remediation of polluted river sediment |
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2016
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| CN1246815A (en) * | 1997-02-07 | 2000-03-08 | 株式会社荏原制作所 | Process for purifying substances polluted with organohalogen compounds |
| JP2005529960A (en) * | 2002-06-21 | 2005-10-06 | ザ ユニバーシティー オブ ニューキャッスル リサーチ アソシエイツ （ティーユーエヌアールエイ）リミテッド | Probiotics, Propionibacterium ienseni 702 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109019819A (en) * | 2018-09-04 | 2018-12-18 | 上海格林曼环境技术有限公司 | For the reduction medicament of chlorohydrocarbon polluted underground water in-situ immobilization and its preparation and application method |
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