CN102815794A - Composite bio-energizer used for in-situ ecological remediation of polluted river sediment - Google Patents
Composite bio-energizer used for in-situ ecological remediation of polluted river sediment Download PDFInfo
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- CN102815794A CN102815794A CN2012103115703A CN201210311570A CN102815794A CN 102815794 A CN102815794 A CN 102815794A CN 2012103115703 A CN2012103115703 A CN 2012103115703A CN 201210311570 A CN201210311570 A CN 201210311570A CN 102815794 A CN102815794 A CN 102815794A
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Abstract
The invention discloses composite bio-energizer used for in-situ ecological remediation of polluted river sediment. The composite bio-energizer comprises oxidant and bio-energizer. The oxidant is one or two of calcium peroxide or calcium nitrate. The bio-energizer comprises amino acid, micro-molecular organic acid, vitamin, inorganic salt and microelement. According to the characteristics of bottom sludge in a river, oxidant and bio-energizer compositions are obtained by screening, matching quality of the oxidant can be adjusted according to pollution level of the bottom sludge in the river, and ecological risk cannot be caused to the bottom sludge due to absence of microorganism. In addition, growth and reproduction of indigenous microorganism are achieved by stimulating the bottom sludge, a pollution-resistant bottom sludge micro-ecosystem is established, and remediation effect of the bottom sludge can be kept excellent for a long term. An experimental research shows that by the use of the composite bio-energizer, content of TOC (total organic carbon) in the bottom sludge can be reduced, and diversity and activity of microorganisms are increased.
Description
Technical field
The invention belongs to the restoration of the ecosystem field of gushing in the river, particularly a kind ofly be used to pollute the compound bio growth-promoting agent that the restoration of the ecosystem of settling original position is gushed in the river.
Background technology
Be used to control flood, natural river course or artificial water canal, the artificial lake etc. of draining, shipping, be known as the river and gush.It is the carrier that nutrition in numerous water, pollutants transfer transform that bed mud is gushed in the river, for the growth of mikrobe and waterplant provides necessary element, is the important step of material cycle in the water ecosystem.
Because the function of urban waterway is special; Admitted a large amount of sewages, factory effluent etc., pollutent gets in the bed mud through the migration of number of ways such as deposition, absorption, bio-absorbable; And constantly accumulated, cause the sediment pollution substrate concentration to exceed the overlying water several magnitude.After bed mud was polluted, a large amount of organic substances mineralising that is decomposed caused the hydrobiont anoxic.Hydrobiological living environment changes, and has limited microbial numbers and kind in the bed mud, degenerates thereby cause the river to gush the ecosystem, and to the shock proof ability drop of pollutent, water burst body blackout in river is smelly.Ceaselessly carrying out the exchange of matter and energy between synchronous deposits and overlying water, the change of environment can cause that the desorb of pollution substance, diffusion get into overlying water again, become " source " of pollutent from " remittance " of pollutent.Therefore, the reparation of bed mud becomes and prevents that the river from gushing the basic place of secondary pollution.
Existing bed mud recovery technique is divided into dystopy recovery technique and based technique for in-situ remediation.The dystopy treatment technology is meant and adopts excavation to handle the bed mud that is polluted that the place that is transported to the various engineerings preparations of process is then repaired.The dystopy recovery technique adopts the measure of system that pollutent is thoroughly cleared out of; Can effectively reduce the pollutent output of bed mud to overlying water; Reduced the risk that secondary pollution is gushed in the river; But because shortcoming such as its expensive, pollutent are residual, destroy the ecotope of water body own, pollution situation occurs repeatedly, can not be from stopping the pollution that gush in the river in essence, so the original position reparation has replaced the dystopy recovery technique gradually and becomes the research focus.Based technique for in-situ remediation is meant and utilizes physics and chemical process or biological method to reduce the volume of Marine sediments, reduces or reduce solubleness, toxicity or the transport property of amount of pollutant and pollutent, thereby reduce the release of pollutent.What use was more in the based technique for in-situ remediation is original position mikrobe recovery technique, original position phytoremediation technology, biology in situ combination recovery technique, in-situ solidifying/stabilization treatment and in-situ chemical processing etc.
The biology in situ recovery technique has following characteristics: at first can reduce effectively because the bed mud resuspending risk that dredging causes reduces the total amount of pollutant that the bed mud ambient shifts; Secondly, the needed expense of based technique for in-situ remediation is lower; At last, the biology in situ recovery technique can permanently reduce the bed mud volume, reduce pollutent toxicity, and can control the migration of pollutent effectively.Therefore the biology in situ recovery technique is just promptly by broad research.
Summary of the invention
The shortcoming that primary and foremost purpose of the present invention is to overcome prior art provides a kind of compound bio growth-promoting agent that the restoration of the ecosystem of settling original position is gushed in the river that is used to pollute with not enough.
Another object of the present invention is to provide the described preparation method that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river that is used to pollute.
A purpose more of the present invention is to provide the described application that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river that is used to pollute.
The object of the invention is realized through following technical proposals: a kind of being used to polluted the compound bio growth-promoting agent that the restoration of the ecosystem of settling original position is gushed in the river, is made up of oxygenant and biological growth promoter;
Described oxygenant is one or both in calcium superoxide or the nitrocalcite; Being preferably quicklime and nitrocalcite obtains by mass ratio 1:1 mixing;
Described biological growth promoter comprises amino acid, small molecular organic acid, VITAMINs, inorganic salt and trace element;
Described biological growth promoter contains following composition: by every liter of calculating, contain Methionin 0.09～0.375g, Isoleucine 0.075～0.325g, tryptophane 0.09～0.325g, glycocoll 0.045～0.09g, L-glutamic acid 0.09～0.375g, glucose 0.006～0.03g, Sodium.alpha.-hydroxypropionate 0.006～0.03g, Trisodium Citrate 0.006～0.03g, wood sugar 0.003～0.012g, D-semi-lactosi 0.003～0.012g, 0.9～6.75mg VITMAIN B1,0.9～6.75mg cobalamin, 1.5～11.25mg vitamins C, NaH
2PO
42.4～7.2g, KCl 1.49～4.48g, NaCl 1.17～3.5g, MgCl
21.9～5.72g, EDTA 9～16.5mg, FeSO
40.9～1.65mg, (NH
4) Mo
7O
40.014～0.039mg, CuSO
40.28～0.48mg, CoCl
20.3～0.51mg, CaCl
21.06～1.74mg, ZnSO
44～7.5mg and MnCl
20.1～0.21mg;
Described biological growth promoter contains following composition: by every liter of calculating, contain Methionin 0.12g, Isoleucine 0.24g, tryptophane 0.24g, glycocoll 0.06g, L-glutamic acid 0.24g, glucose 0.0084g, Sodium.alpha.-hydroxypropionate 0.0168g, Trisodium Citrate 0.0168g, wood sugar 0.0042g, D-semi-lactosi 0.0042g, 1.8mg VITMAIN B1,1.8mg cobalamin, 3mg vitamins C, NaH
2PO
43g, KCl 1.86g, NaCl 1.46g, MgCl
22.38g, EDTA 12.5mg, FeSO
41.225mg, (NH
4) Mo
7O
40.0275mg, CuSO
40.39mg, CoCl
20.4mg, CaCl
21.39mg, ZnSO
45.5mg and MnCl
20.15mg;
Described being used to polluted the preparation method that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river, may further comprise the steps:
(1) preparation of biological growth promoter:
1. the preparation of amino acid solution: respectively Methionin, Isoleucine, tryptophane, glycocoll and L-glutamic acid are mixed with the solution that concentration is 15～25mg/mL;
2. the preparation of small molecular organic acid solution: respectively glucose, Sodium.alpha.-hydroxypropionate, Trisodium Citrate, wood sugar and D-semi-lactosi are mixed with the solution that concentration is 1.0～2.0g/L;
3. the preparation of vitamin solution: VITMAIN B1, B12 and C are mixed with mixing solutions, and wherein every kind of vitamine concentration is 0.15 ~ 0.75mg/mL;
4. the preparation of inorganic salt solution: with NaH
2PO
4, KCl, NaCl and MgCl
2Be mixed with solution, the concentration of each material is 1～2mol/L in the solution;
5. the preparation of trace element solution: calculate by every liter of trace element solution, contain EDTA 450～550mg, FeSO
445～55mg, (NH
4) Mo
7O
40.7～1.3mg, CuSO
414～16mg, CoCl
215～17mg, CaCl
253～58mg, ZnSO
4200～250mg, MnCl
25～7mg;
6. the preparation of biological growth promoter: each component that will prepare mixes; Obtain biological growth promoter, each component volume percent is following: trace element solution 2.0～3.0% and water surplus that the inorganic salt solution 2.0～3.0% that the VITAMINs mixing solutions 0.6～1.5% that the D-galactose solution 0.3～0.6% that the xylose solution 0.3～0.6% that the sodium citrate soln 0.6～1.5% that the sodium lactate solution 0.6～1.5% that the glucose solution 0.6～1.5% that the glutamic acid solution 0.6～1.5% that the glycine solution 0.3～0.6% that the tryptophane solution 0.6～1.3% that the Isoleucine solution 0.5～1.3% that the lysine solution 0.6～1.5% that step (1) is 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 3. prepared, step (1) are 4. prepared, step (1) are 5. prepared;
When (2) using, detect river to be repaired and gush the organic content of bed mud; The organic content of gushing bed mud with the river is a benchmark, and the consumption of oxygenant is a mass percent 10%, during use oxygenant is formulated as the aqueous solution; Biological growth promoter is a benchmark with the overlying water volume then, and the consumption of whenever going up the biological growth promoter that covers water body is 120～250 μ L; By the consumption of aforementioned oxygenant and the consumption of biological growth promoter, oxygenant and biological growth promoter is composite, obtain being used to pollute the compound bio growth-promoting agent that the restoration of the ecosystem of settling original position is gushed in the river.
The concentration of step (1) described in 1. is preferably 20mg/mL;
The concentration of step (1) described in 2. is preferably 1.4g/L;
Mikrobe B1 and the B12 concentration of step (1) described in 3. is preferably 0.3mg/mL, and ascorbic concentration is preferably 0.6mg/mL;
The concentration of step (1) described in 4. is preferably 1mol/L;
The trace element solution of step (1) described in 5. more preferably calculates by every liter of trace element solution, contains EDTA 500mg, FeSO
449mg, (NH
4) Mo
7O
41.1mg, CuSO
415.7mg, CoCl
216.1mg, CaCl
255.4mg, ZnSO
4220mg, MnCl
26mg;
The biological growth promoter of step (1) described in 6. more preferably, each component volume percent is following: trace element solution 2.5% and water surplus that the inorganic salt solution 2.5% that the VITAMINs mixing solutions 0.6% that the D-galactose solution 0.3% that the xylose solution 0.3% that the sodium citrate soln 1.2% that the sodium lactate solution 1.2% that the glucose solution 0.6% that the glutamic acid solution 1.2% that the glycine solution 0.3% that the tryptophane solution 1.2% that the Isoleucine solution 1.2% that the lysine solution 0.6% that step (1) is 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 3. prepared, step (1) are 4. prepared, step (1) are 5. prepared;
Described being used to polluted the application that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river, comprises the steps:
(1), confirms each component dosage of complex microorganism preparations, and mix according to bed mud organic content and overlying water volume;
When (2) using, will be set by step (1) compound bio growth-promoting agent solution of preparing evenly splash in the bed mud top layer;
Be used to pollute the application that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river, more preferably comprise the steps:
(1), confirms each component dosage of complex microorganism preparations, and mix according to bed mud organic content and overlying water volume;
When (2) using, will be set by step (1) compound bio growth-promoting agent solution of preparing evenly splash in the bed mud top layer; The compound bio growth-promoting agent that described (1) set by step that adds a half-value dose two days later prepares.
The present invention has following advantage and effect with respect to prior art:
Bed mud is the important ring that material cycle is gushed in the river, and the microbial process in the bed mud has very important effect to the migration conversion of pollutent.The redox environment of bed mud has influenced the little ecologic structure of bed mud, also influenced the form that pollutants in sediments exists simultaneously, and the integrity of bed mud microecosystem has influenced the degradation capability of bed mud to pollutent.Existing result of study shows that the raising key of urban waterway self-purification capacity is in the reparation of bed mud.The present invention is directed to the river and gush the bed mud characteristics, screening obtains oxygenant and biological growth promoter composition, can gush the adjustment that the sediment pollution degree is carried out oxygenant proportioning quality according to the river, because do not contain mikrobe, can not cause ecological risk to bed mud.Through stimulating the growth and breeding of bed mud indigenous microorganism, set up resistance to soiling bed mud microecosystem simultaneously, made the repairing effect of bed mud in long period, maintain state preferably.Experimental study shows, uses the compound bio growth-promoting agent among the present invention, can make TOC content decline in the bed mud, microbial diversity and active the rising.
Description of drawings
Fig. 1 adds the compound bio growth-promoting agent to bottom mud microbe activity influence figure.
Fig. 2 adds the influence figure of compound bio growth-promoting agent to bed mud TOC content.
Fig. 3 adds the compound bio growth-promoting agent microbial diversity exponential is influenced figure.
Embodiment
Below in conjunction with embodiment and accompanying drawing the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1
Adopt the Guangzhou to pollute the river and gush bed mud (Pa continent bridge section bed mud) for repairing object; Leading indicator is: overlying water dissolved oxygen (DO) is 1.6mg/L; Bed mud redox potential (ORP) is-220mv; The bed mud water ratio is 41.2%, and organic (TOC) content of bed mud is 2.92%, and it is 0.61A/g that the FDA method is measured the bottom mud microbe gross activity.
Adopting BIOLOG ECO microplate method structure microbial diversity index (Shannon index) (background) simultaneously is 3.0.
It is experimental installation that the uncovered synthetic glass cylinder of 55cm * 35cm * 20cm is adopted in experiment, lays the 1.2cm bed mud, and quality is 1350g, and adding the 4L tap water is overlying water.
The preparation method is following for the compound bio growth-promoting agent:
1. the preparation of amino acid solution: water is formulated as Methionin, Isoleucine, tryptophane, glycocoll, L-glutamic acid the solution of 20mg/mL respectively;
2. the preparation of small molecular organic acid solution: water is formulated as glucose, Sodium.alpha.-hydroxypropionate, Trisodium Citrate, wood sugar, D-semi-lactosi the solution of 1.4g/L respectively;
3. the preparation of vitamin solution: water is made into the VITAMINs mixing solutions with VITMAIN B1, B12, C; Wherein VITMAIN B1 and B12 concentration are 0.3mg/mL, and ascorbic concentration is 0.6mg/mL;
4. the preparation of inorganic salt solution: water is with NaH
2PO
4, KCl, NaCl, MgCl
2, preparation obtains the macroelement solution of 1mol/L;
5. the preparation of trace element solution: the water preparation, calculate by every liter of trace element solution, contain EDTA500mg, FeSO
449mg, (NH
4) Mo
7O
41.1mg, CuSO
415.7mg, CoCl
216.1mg, CaCl
255.4mg, ZnSO
4220mg, MnCl
26mg;
6. the preparation of biological growth promoter: each component mixed diluting that will prepare is to certain volume; Be biological growth promoter, each component volume percent is following: Methionin 0.6%, Isoleucine 1.2%, tryptophane 1.2%, glycocoll 0.3%, L-glutamic acid 1.2%, glucose 0.6%, Sodium.alpha.-hydroxypropionate 1.2%, Trisodium Citrate 1.2%, wood sugar 0.3%, D-semi-lactosi 0.3%, VITAMINs 0.6%, macroelement 2.5%, trace element 2.5% and water 86.3%.
The dosage that calculates calcium superoxide and nitrocalcite according to bed mud quality and TOC content (wet shale amount * (1-bed mud water ratio) * organic content * 0.05) is 1.15g; Be dissolved in the 10mL zero(ppm) water; The biological growth promoter dosage is selected 250 μ L/L overlying waters, promptly adds 1mL.
Experimental group and control group (growth-promoting agent blank control group) are set.Experimental group adds the compound bio growth-promoting agent, the compound bio growth-promoting agent of 11mL is evenly splashed in the bed mud top layer in the 1st day, after 2 days, adds the compound bio growth-promoting agent of the 1st a day half-value dose; Control group does not add biological growth promoter.Experiment through 10 days; Control group water body dissolved oxygen content drops to 2.5mg/L by 7.0mg/L; The bed mud redox potential drops to-240mV by-220; Experimental group overlying water dissolved oxygen content maintains 6mg/L, and redox potential rises to-and the 110mv reducing environment improves, and the bed mud color changes pearl into gradually by black; Control group experiment bottom mud microbe FDA gross activity is reduced to 0.58A/g by 0.61A/g, and experimental group rises to 1.0A/g; Control group Shannon index is 2.94, and experimental group Shannon index raises most 3.25, and microbial diversity improves; Control group bed mud TOC content is reduced to 2.85% by 2.92%, and experimental group bed mud TOC content is reduced to 2.25%, sees Fig. 1,2 and 3 (among the figure, background refers to test the background value of the bed mud parameters of adopting).Therefore adding the compound bio growth-promoting agent can effectively improve the bed mud reducing environment, promotes microorganism active and multifarious rising, helps the degraded of bed mud TOC.
Adopt oxygenant and the composite mode of biological growth promoter to carry out the preparation of compound bio growth-promoting agent; Through adding of compound bio growth-promoting agent, under the effect of the slow oxygen release of calcium superoxide, overlying water DO content rising bed mud redox potential improves; The bottom mud microbe structure of community develops; The bed mud color develops into pearl by black gradually, and to add nitrate salt be that bed mud provides electron acceptor(EA), stimulates the activity of bed mud denitrifying bacteria to raise; Promote denitrification to produce nitrogen, so have small bubbles to produce in the bed mud.Simultaneously under the promoter action of biological growth promoter; Bottom mud microbe is bred in a large number, microbial diversity and active the rising, and it is organic to decompose bed mud; Absorb materials such as ammonia nitrogen and phosphoric acid salt simultaneously and carry out the synthetic of organism, and the existence of calcium ion has suppressed nutritive salt and organic release.Fundamentally eliminate the river and gushed the phenomenon of deceiving smelly and eutrophication.
Embodiment 2
Adopt the Guangzhou to pollute the river and gush bed mud (Pa continent bridge section bed mud) for repairing object; Leading indicator is: overlying water dissolved oxygen (DO) is 1.6mg/L; Bed mud redox potential (ORP) is-220mv; The bed mud water ratio is 41.2%, and organic (TOC) content of bed mud is 2.92%, and it is 0.61A/g that the FDA method is measured the bottom mud microbe gross activity.
Adopting BIOLOG ECO microplate method structure microbial diversity index (Shannon index) simultaneously is 3.0.
It is experimental installation that the uncovered synthetic glass cylinder of 55cm * 35cm * 20cm is adopted in experiment, lays the 1.2cm bed mud, and quality is 1171.6g, and adding the 4L tap water is overlying water.
The preparation method is following for the compound bio growth-promoting agent:
1. amino acid whose preparation: the solution that respectively Methionin, Isoleucine, tryptophane, glycocoll, L-glutamic acid is formulated as 20mg/mL;
2. the preparation of small molecular organic acid: the solution that respectively glucose, Sodium.alpha.-hydroxypropionate, Trisodium Citrate, wood sugar, D-semi-lactosi is formulated as 1.4g/L;
3. the preparation of vitamin solution: water is made into the VITAMINs mixing solutions with VITMAIN B1, B12, C; Wherein VITMAIN B1 and B12 concentration are 0.3mg/mL, and ascorbic concentration is 0.6mg/mL;
4. the preparation of inorganic salt solution: with NaH
2PO
4, KCl, NaCl, MgCl
2, preparation obtains the solution of 1mol/L;
5. the preparation of trace element solution: calculate by every liter of trace element solution, contain EDTA 500mg, FeSO
449mg, (NH
4) Mo
7O
41.1mg, CuSO
415.7mg, CoCl
216.1mg, CaCl
255.4mg, ZnSO
4220mg, MnCl
26mg;
6. the preparation of biological growth promoter: each component mixed diluting that will prepare is to certain volume; Be biological growth promoter, each component volume percent is following: Methionin 0.6%, Isoleucine 1.2%, tryptophane 1.2%, glycocoll 0.3%, L-glutamic acid 1.2%, glucose 0.6%, Sodium.alpha.-hydroxypropionate 1.2%, Trisodium Citrate 1.2%, wood sugar 0.3%, D-semi-lactosi 0.3%, VITAMINs 0.6%, macroelement 2.5%, trace element 2.5% and water 86.3%.
The dosage that obtains calcium superoxide and nitrocalcite according to bed mud quality and TOC cubage is 1.0g, is dissolved in the 10mL zero(ppm) water, and the biological growth promoter dosage is selected 120 μ L/L overlying waters, promptly adds 0.5mL.
Experiments experiment group and control group.Experimental group and control group (growth-promoting agent blank control group) are set.Experimental group adds the compound bio growth-promoting agent, the compound bio growth-promoting agent of 10.5mL is evenly splashed in the bed mud top layer in the 1st day, after 2 days, adds the compound bio growth-promoting agent of the 1st a day half-value dose; Control group does not add biological growth promoter.Experiment through 10 days; Control group water body dissolved oxygen content drops to 1.3mg/L by 7.0mg/L; The bed mud redox potential drops to-250mV by-220; Experimental group overlying water dissolved oxygen content maintains 4mg/L, and redox potential rises to-and the 180mv reducing environment improves, and top layer bed mud color changes pearl into gradually by black; Control group experiment bottom mud microbe FDA gross activity drops to 0.52A/g by 0.61A/g, and experimental group rises to 0.83A/g; Control group Shannon index is reduced to 2.94, and experimental group Shannon index is increased to 3.37 by 3.0, and microbial diversity improves; Control group bed mud TOC content is reduced to 2.90% by 2.92%, and experimental group bed mud TOC content is reduced to 2.47%.Therefore adding the compound bio growth-promoting agent can effectively improve the bed mud reducing environment, promotes microorganism active and multifarious rising, helps the degraded of bed mud TOC.
The comparative example:
To obtain comparatively suitable bed mud oxygenant in order screening, to have investigated multiple bed mud oxygenant and growth-promoting agent the bed mud repairing effect.Except calcium superoxide and nitrocalcite, also carried out hydrogen peroxide and biological growth promoter Bio-energizer (BE) research to the bed mud repairing effect.Process and result are following:
Adopt the Guangzhou to pollute the river and gush bed mud (Pa continent bridge section bed mud) for repairing object; Leading indicator is: overlying water dissolved oxygen (DO) is 1.6mg/L; Bed mud redox potential (ORP) is-220mv; The bed mud water ratio is 41.2%, and organic (TOC) content of bed mud is 2.92%, and bottom mud microbe gross activity (FDA) is 0.61A/g.
Adopting BIOLOG ECO microplate method structure microbial diversity index (Shannon index) is 3.0.
It is experimental installation that the uncovered synthetic glass cylinder of 55cm * 35cm * 20cm is adopted in experiment, lays the 1.5cm bed mud, and quality is 1200g, and adding the 4L tap water is overlying water.
Adopt hydrogen peroxide and BE to carry out the bed mud reparation respectively, concrete dosage is following: hydrogen peroxide is an oxygenant, and 5% add according to the bed mud organic content promptly adds 0.98g; BE then to specifications requirement adds according to the 15ppm of overlying water volume, i.e. 60 μ L.
Test setting adds oxygenant experimental group and control experiment group.Experimental group adds hydrogen peroxide and BE respectively; Control group does not then add any growth-promoting agent.The result shows, adds under the situation of hydrogen peroxide, and the overlying water dissolved oxygen rose to 8mg/L by 1.6mg/L in one day; Then be reduced to gradually below the 1mg/L, redox potential earlier is increased to-is reduced to-230mv behind the 70mv by-220mv, and bed mud becomes pearl by black; Volume increases, since the strong oxidation of hydrogen peroxide, the fluffy come-up of bed mud; Overlying water is muddy; Bed mud FDA is reduced to 0.44A/g from 0.61A/g and is lower than control group 0.58A/g, and microorganism active is produced restraining effect, is unfavorable for that the original position mikrobe of bed mud is repaired.Add under the situation of BE, bed mud ORP is increased to-200mv, and bed mud FDA activity rises to 0.8A/g by 0.61A/g; The Shannon index is increased to 3.11 a little more than control group 2.94 by 3.0, does not have significant difference with respect to control group and background value, and TOC content then is reduced to 2.51% by 2.92%; As shown in Figure 2, though being described, BE helps to improve the bottom mud microbe activity, the degradation effect that promotes bed mud TOC is not so good as the effect of compound bio growth-promoting agent provided by the invention.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. one kind is used to pollute the compound bio growth-promoting agent that the restoration of the ecosystem of settling original position is gushed in the river, it is characterized in that: describedly be used to pollute the compound bio growth-promoting agent that gushes the restoration of the ecosystem of settling original position in the river and be made up of oxygenant and biological growth promoter; Described oxygenant is one or both in calcium superoxide or the nitrocalcite; Described biological growth promoter comprises amino acid, small molecular organic acid, VITAMINs, inorganic salt and trace element.
2. according to claim 1 being used to polluted the compound bio growth-promoting agent that the restoration of the ecosystem of settling original position is gushed in the river, it is characterized in that: described oxygenant is that quicklime and nitrocalcite obtain by mass ratio 1:1 mixing.
3. according to claim 1ly be used to pollute the compound bio growth-promoting agent that the restoration of the ecosystem of settling original position is gushed in the river; It is characterized in that: described biological growth promoter contains following composition: by every liter of calculating, contain Methionin 0.09～0.375g, Isoleucine 0.075～0.325g, tryptophane 0.09～0.325g, glycocoll 0.045～0.09g, L-glutamic acid 0.09～0.375g, glucose 0.006～0.03g, Sodium.alpha.-hydroxypropionate 0.006～0.03g, Trisodium Citrate 0.006～0.03g, wood sugar 0.003～0.012g, D-semi-lactosi 0.003～0.012g, 0.9 ~ 6.75mg VITMAIN B1,0.9 ~ 6.75mg cobalamin, 1.5～11.25mg vitamins C, NaH
2PO
42.4～7.2g, KCl 1.49～4.48g, NaCl 1.17～3.5g, MgCl
21.9～5.72g, EDTA 9～16.5mg, FeSO
40.9～1.65mg, (NH
4) Mo
7O
40.014～0.039mg, CuSO
40.28～0.48mg, CoCl
20.3～0.51mg, CaCl
21.06～1.74mg, ZnSO
44～7.5mg and MnCl
20.1～0.21mg.
4. according to claim 3ly be used to pollute the compound bio growth-promoting agent that the restoration of the ecosystem of settling original position is gushed in the river; It is characterized in that: described biological growth promoter contains following composition: by every liter of calculating, contain Methionin 0.12g, Isoleucine 0.24g, tryptophane 0.24g, glycocoll 0.06g, L-glutamic acid 0.24g, glucose 0.0084g, Sodium.alpha.-hydroxypropionate 0.0168g, Trisodium Citrate 0.0168g, wood sugar 0.0042g, D-semi-lactosi 0.0042g, 1.8mg VITMAIN B1,1.8mg cobalamin, 3mg vitamins C, NaH
2PO
43g, KCl 1.86g, NaCl 1.46g, MgCl
22.38g, EDTA 12.5mg, FeSO
41.225mg, (NH
4) Mo
7O
40.0275mg, CuSO
40.39mg, CoCl
20.4mg, CaCl
21.39mg, ZnSO
45.5mg and MnCl
20.15mg.
5. claim 1～3 each described being used to is polluted the preparation method that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river, it is characterized in that may further comprise the steps:
(1) preparation of biological growth promoter:
1. the preparation of amino acid solution: respectively Methionin, Isoleucine, tryptophane, glycocoll and L-glutamic acid are mixed with the solution that concentration is 15～25mg/mL;
2. the preparation of small molecular organic acid solution: respectively glucose, Sodium.alpha.-hydroxypropionate, Trisodium Citrate, wood sugar and D-semi-lactosi are mixed with the solution that concentration is 1.0～2.0g/L;
3. the preparation of vitamin solution: VITMAIN B1, B12 and C are mixed with the VITAMINs mixing solutions, and wherein the concentration of every kind of VITAMINs is 0.15～2.5mg/mL;
4. the preparation of inorganic salt solution: with NaH
2PO
4, KCl, NaCl and MgCl
2Be mixed with macroelement solution, the concentration of each material is 1～2mol/L in the solution;
5. the preparation of trace element solution: calculate by every liter of trace element solution, contain EDTA 450～550mg, FeSO
445～55mg, (NH
4) Mo
7O
40.7～1.3mg, CuSO
414～16mg, CoCl
215～17mg, CaCl
253～58mg, ZnSO
4200～250mg, MnCl
25～7mg;
6. the preparation of biological growth promoter: each component that will prepare mixes; Obtain biological growth promoter, each component volume percent is following: trace element solution 2.0～3.0% and water surplus that the macroelement solution 2.0～3.0% that the VITAMINs mixing solutions 0.6～1.5% that the D-galactose solution 0.3～0.6% that the xylose solution 0.3～0.6% that the sodium citrate soln 0.6～1.5% that the sodium lactate solution 0.6～1.5% that the glucose solution 0.6～1.5% that the glutamic acid solution 0.6～1.5% that the glycine solution 0.3～0.6% that the tryptophane solution 0.6～1.3% that the Isoleucine solution 0.5～1.3% that the lysine solution 0.6～1.5% that step (1) is 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 3. prepared, step (1) are 4. prepared, step (1) are 5. prepared;
When (2) using, detect river to be repaired and gush the organic content of bed mud; The organic content of gushing bed mud with the river is a benchmark, and the consumption of oxygenant is a mass percent 10%, during use oxygenant is formulated as the aqueous solution; Biological growth promoter is a benchmark with the overlying water volume then, and the consumption of whenever going up the biological growth promoter that covers water body is 120～250 μ L; By the consumption of aforementioned oxygenant and the consumption of biological growth promoter, oxygenant and biological growth promoter is composite, obtain being used to pollute the compound bio growth-promoting agent that the restoration of the ecosystem of settling original position is gushed in the river.
6. according to claim 5 being used to polluted the preparation method that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river, it is characterized in that:
The concentration of step (1) described in 1. is 20mg/mL;
The concentration of step (1) described in 2. is 1.4g/L;
Vitamins B 1 and the B12 concentration of step (1) described in 3. is 0.3mg/mL, and ascorbic concentration is 0.6mg/mL;
The concentration of step (1) described in 4. is 1mol/L.
7. according to claim 6ly be used to pollute the preparation method that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river; It is characterized in that: the trace element solution of step (1) described in 5. does; Calculate by every liter of trace element solution, contain EDTA 500mg, FeSO
449mg, (NH
4) Mo
7O
41.1mg, CuSO
415.7mg, CoCl
216.1mg, CaCl
255.4mg, ZnSO
4220mg, MnCl
26mg.
8. according to claim 6ly be used to pollute the preparation method that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river; It is characterized in that: the biological growth promoter of step (1) described in 6. is that each component volume percent is following: trace element solution 2.5% and water surplus that the inorganic salt solution 2.5% that the VITAMINs mixing solutions 0.6% that the D-galactose solution 0.3% that the xylose solution 0.3% that the sodium citrate soln 1.2% that the sodium lactate solution 1.2% that the glucose solution 0.6% that the glutamic acid solution 1.2% that the glycine solution 0.3% that the tryptophane solution 1.2% that the Isoleucine solution 1.2% that the lysine solution 0.6% that step (1) is 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 1. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 2. prepared, step (1) are 3. prepared, step (1) are 4. prepared, step (1) are 5. prepared.
9. claim 1～4 each described being used to is polluted the application that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river, it is characterized in that comprising the steps:
(1), confirms each component dosage of complex microorganism preparations, and mix according to bed mud organic content and overlying water volume;
When (2) using, will be set by step (1) compound bio growth-promoting agent solution of preparing evenly splash in the bed mud top layer.
10. according to claim 9 being used to polluted the application that the compound bio growth-promoting agent of settling original position restoration of the ecosystem is gushed in the river, it is characterized in that comprising the steps:
(1), confirms each component dosage of complex microorganism preparations, and mix according to bed mud organic content and overlying water volume;
When (2) using, will set by step (1) compound bio growth-promoting agent solution of preparing lead to and evenly splash in the bed mud top layer; The compound bio growth-promoting agent that (1) set by step of the described half-value dose of equally evenly splashing two days later prepares.
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| CN103332836A (en) * | 2013-06-09 | 2013-10-02 | 华南农业大学 | Denitrification sulphur removal-based in-situ black-odorous riverway bottom mud oxidization technology |
| CN104445640A (en) * | 2014-11-27 | 2015-03-25 | 新疆德蓝股份有限公司 | Process for preparing bio-stimulant by utilizing swill |
| CN105417713A (en) * | 2015-12-11 | 2016-03-23 | 杭州银江环保科技有限公司 | Comprehensive maintaining method for submerged plants |
| CN106587342A (en) * | 2016-12-29 | 2017-04-26 | 中国科学院城市环境研究所 | Bioremediation reagent and application thereof in removing 1,2-dichloroethane in composite polluted water body |
| CN106830351A (en) * | 2017-01-04 | 2017-06-13 | 上田环境修复股份有限公司 | A kind of bio-stimulant repaired for river course, lake, landscape water body and its preparation method and application |
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| CN108529759A (en) * | 2018-04-10 | 2018-09-14 | 北京华景山水生态科技有限公司 | A kind of water pollution sediment in-situ is biological reinforced to be handled and Benthic ecology restorative procedure |
| CN108558001A (en) * | 2017-12-28 | 2018-09-21 | 上海交通大学 | For in-situ immobilization polluted-water and the biological exciting agent and its application method of bed mud |
| CN109319946A (en) * | 2018-10-30 | 2019-02-12 | 深圳大学 | A kind of microorganism growth stimulant and preparation method thereof |
| CN109534631A (en) * | 2017-09-21 | 2019-03-29 | 天津大学 | A kind of compound bed mud improver and preparation method thereof for administering black-odor riverway |
| CN109534632A (en) * | 2017-09-21 | 2019-03-29 | 天津大学 | A kind of submerged plant-improver joint improves the in-situ remediation method of black-odor riverway bed mud |
| CN110240371A (en) * | 2019-07-15 | 2019-09-17 | 北京万水净水剂有限公司 | A kind of black and odorous water sediment in-situ modification method |
| CN110862153A (en) * | 2018-08-28 | 2020-03-06 | 河北满福栖农业科技有限公司 | Biological growth promoter |
| CN114477675A (en) * | 2022-02-15 | 2022-05-13 | 中国水利水电第六工程局有限公司 | Repairing agent and method for in-situ treatment of transfer of black and odorous lake sediment pollutants to overlying water |
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| CN103332836B (en) * | 2013-06-09 | 2014-09-24 | 华南农业大学 | Denitrification sulphur removal-based in-situ black-odorous riverway bottom mud oxidization technology |
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| CN108103000A (en) * | 2017-12-27 | 2018-06-01 | 齐鲁工业大学 | A kind of growth promoter for improving anaerobic ammonia oxidizing bacteria and preparation method thereof |
| CN108558001A (en) * | 2017-12-28 | 2018-09-21 | 上海交通大学 | For in-situ immobilization polluted-water and the biological exciting agent and its application method of bed mud |
| CN108529759A (en) * | 2018-04-10 | 2018-09-14 | 北京华景山水生态科技有限公司 | A kind of water pollution sediment in-situ is biological reinforced to be handled and Benthic ecology restorative procedure |
| CN110862153A (en) * | 2018-08-28 | 2020-03-06 | 河北满福栖农业科技有限公司 | Biological growth promoter |
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