CN106578661A - Heat clearing and detoxification trifolium repens l protein powder feed for crucian breeding in summer, and preparation method thereof - Google Patents
Heat clearing and detoxification trifolium repens l protein powder feed for crucian breeding in summer, and preparation method thereof Download PDFInfo
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- CN106578661A CN106578661A CN201610984179.8A CN201610984179A CN106578661A CN 106578661 A CN106578661 A CN 106578661A CN 201610984179 A CN201610984179 A CN 201610984179A CN 106578661 A CN106578661 A CN 106578661A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses a heat clearing and detoxification trifolium repens l protein powder feed for crucian breeding in summer, and a preparation method thereof, wherein the heat clearing and detoxification trifolium repens l protein powder feed is prepared from the following raw materials by weight: 800-850 parts of trifolium repens l, 0.15-0.18 part of cellulase, 0.12-0.15 part of pectinase, 0.1-0.12 part of protease, 1.3-1.5 parts of folium isatidis, 1.6-1.8 parts of honeysuckle, 1.2-1.4 parts of forsythia suspensa, 0.4-0.6 part of andrographis paniculata, 0.5-0.7 part of rubus corchorifolius l. f juice, 3-4 parts of chicken hydrolyzate, 17-21 parts of gluten powder, 3-4 parts of cyanobacteria fermentation product, 1-1.2 parts of cherry flower, 0.7-0.8 part of curcuma zedoaria, 14-16 parts of mushroom powder, 7-8 parts of chicken intestine powder, 2-3 parts of ixeris sonchifolia powder, 34-37 parts of peanut meal, 3-5 parts of yeast culture, and 2-3 parts of leek juice. The heat clearing and detoxification trifolium repens l protein powder feed of the present invention provides effects of heat clearing and detoxification in the crucian breeding production process in summer, and has effects of crucian immune function enhancing, disease resistance enhancing, and stress resistance enhancing.
Description
Technical field
The present invention relates to fishery -ies product cultural technique field, more particularly to a kind of summer crucian cultivation heat-clearing and toxic substances removing Trifolium repense
Albumen powder feedstuff and preparation method thereof.
Background technology
It is global that protein lacks, and the blade of higher plant is considered as most promising protein resource, plant
Albumen is because of its cheap, aboundresources and can meet the demand of the mankind and domestic animal to protein, causes people and greatly pays close attention to.Open
Sending out plant protein resource becomes one problem demanding prompt solution of grocery trade and feed industry.Leaf protein feedstuff, be otherwise known as green
Protein concentrate, refers to fresh herbage or other dark green plants as raw material, and Jing after squeezing, the concentration extracted from its juice is thick
Protein product, it is mainly made up of cytoplasm protein and chloroplast stroma albumen.With animal husbandry, culture fishery it is rapid
The effectively utilizes of development, herbage and other dark green plants are taken seriously.As the nonruminants such as pig, fowl are to green forage albumen
The utilization rate of matter is relatively low, not only causes albumen waste, and have impact on effective absorption of the poultry to nutritional labeling.Therefore will herd
Protein Extraction in grass and green forage out, as albumen feedstuff, can make up the shortage of China's forage protein, to herding
The high speed of industry, sound development have important facilitation.So the production of leaf protein feedstuff have wide development space and
Application prospect.Trifolium repense is a kind of leguminous forage, is mainly used in grassland construction in China, with good ecological and economic valency
Value, its green grass or young crops raise period of use length, gross protein value height, extensive management, soft delicacy, are important planting forage grass and Ornamental turf
Grass.At present, the research and development with regard to Trifolium repense leaf protein is also very rare.《Trifolium repense leaf protein is extracted and purifying process》One
Though the method disclosed in text has certain practical value, as its extraction efficiency is low and technique backwardness is rotten, it is impossible to make extraction
Leaf protein out obtains more preferable quality, taste and flavor improvement, can not realize the conditioning to aquatic fish physiological function
And regulation, it is unfavorable for the raising of aquatic fish production performance and culture benefit.Therefore, the present invention has done improvement process, not only carries
The high extraction efficiency of leaf protein, increased leaf protein yield, further improves growth, the production performance of aquatic fish, improve
The economic benefit of cultivation.
The content of the invention
In order to solve the deficiencies in the prior art, the invention provides a kind of summer crucian cultivation heat-clearing and toxic substances removing Trifolium repense egg
White lead feedstuff and preparation method thereof.
The present invention is achieved by the following technical solutions：
A kind of summer crucian cultivation heat-clearing and toxic substances removing Trifolium repense albumen powder feedstuff, is prepared by the raw materials in：Trifolium repense
800-850, cellulase 0.15-0.18, pectase 0.12-0.15, proteinase-10 .1-0.12, Folium Isatidiss 1.3-1.5, Flos Lonicerae
1.6-1.8, Fructus Forsythiae 1.2-1.4, Herba Andrographis 0.4-0.6, raspberry juice 0.5-0.7, hydrolysis of chicken meat thing 3-4, gluten meal 17-21, indigo plant
Algae fermented product 3-4, cherry blossom 1-1.2, Rhizoma Curcumae 0.7-0.8, mushroom powder 14-16, chicken rice noodle roll 7-8, gutweed vegetable powder 2-3, Semen arachidis hypogaeae dregs
34-37, yeast culture 3-5, Chinese chive juice 2-3.
Described summer crucian cultivation heat-clearing and toxic substances removing Trifolium repense albumen powder feedstuff, its preparation method are as follows：
（1）The white clover leaves of fresh, no disease and pests harm are gathered, shearing crushing is carried out after cleaning up, by the Trifolium repense after crushing
Mixing according to the ratio that weight compares 1: 10-15 with water carries out rotation beating at a high speed, arranges beater rotating speed 1800-2400r/min,
The homogenate for obtaining is heated to into 52-56 DEG C, then using ultrasonic-leaching, setting ultrasonic frequency is 3000-5000KHz, leaching
The time is carried for 20-25min, solid-liquid separation after ultrasonic-leaching, is carried out to homogenate, leaching leaf juice is concentrated and is spray-dried,
Obtain the first extract powder；
（2）By step（1）Leaf slag after solid-liquid separation is put and is dried in the shade at room temperature, carries out micronizing, smashing fineness 200-300
Mesh, the regulation that then adds water make its water content reach 15-18%, in 56-58 DEG C of extrusion temperature, screw speed 320-350r/min bars
Extruding puffing under part, will be expanded after the water of 8-10 times of Trifolium repense micropowder and its weight portion mixed, put in enzymatic vessel,
Keep tank temperature to be 43-46 DEG C, then cellulase, pectase and protease stirring and evenly mixing are added in tank, in 70-90r/min bars
Under part, stirring enzymolysis 18-20h, is centrifuged supernatant to the reactant liquor after enzymolysis, by supernatant concentration and is spray-dried,
Obtain the second extract powder；
（3）The water of 5-6 times of weight portion is added persistently to decoct 1-2h after Folium Isatidiss, Flos Lonicerae, Fructus Forsythiae, Herba Andrographis are pulverized and mixed,
Decoction liquor and raspberry juice, hydrolysis of chicken meat thing stirring and evenly mixing are taken, 3-4h in being put into 84-86 DEG C of couveuse, is incubated, is taken out, with Wheat Gluten
Powder, the mixing of blue algae fermentation thing are mixed thoroughly, and heat aging 20-30min obtains modified adjuvant；
（4）Cherry blossom, Rhizoma Curcumae are decocted into 50-60min with the water heating of 3-4 times of weight portion, then step are added to the inside（1）、
Step（2）Extract powder and step（3）The mixing of modified adjuvant, holdings water content is 32-36%, 58-62 DEG C of insulation reaction 40-
50min, obtains modified Trifolium repense egg albumen powder；
（5）5-8 times of water of mushroom powder and its weight portion is mixed and is put in pot, decocted boiling, add chicken rice noodle roll, gutweed vegetable powder
Mix thoroughly, stewing 12-15min take out, mix with Semen arachidis hypogaeae dregs, steam 40-50min, place into carry out in bulking machine it is expanded, setting it is expanded
Temperature 130-150 DEG C, expanded time 2-3s, obtains modified and reinforced dose；
（6）By step（4）、（5）Obtained material is mixed, and adds the water stirring and evenly mixing of gross weight 12-15 times, Ran Houjia
Enter in reactor, arrange reactor temperature be 72-75 DEG C, speed of agitator be 130-150r/min, insulated and stirred 2-3h, then
Other surplus stocks are added, and high-temperature are risen to 94-98 DEG C of insulation reaction 12-15min, are cooled the temperature to rapidly 18- afterwards again
22 DEG C, reactant liquor is centrifuged supernatant concentration after remove impurity and is spray-dried using centrifuge, obtain final products.
Beneficial effects of the present invention：
The present invention can strengthen the immunization machine of Carassius auratuss for the effect in the crucian cultivation production process of summer with heat-clearing and toxic substances removing
Can, strengthen its disease-resistant, anti-stress ability, improve premunition, and the physiological function of Carassius auratuss can be nursed one's health comprehensively, promote which to give birth to
Long development, improves cultured output and survival rate, the features such as feedstuff has natural health, good palatability, prepares easy.Institute of the present invention
Raw material Trifolium repense has huge yield in China, is easy to large-scale production, and from Trifolium repense extracting egg albumen powder not only can
The shortage of China's forage protein is made up, comprehensive exploitation and utilization to China's Trifolium repense resource can also be realized.Trifolium repense albumen
Powder is a kind of protein resource of environmental protection, with good application prospect, substitutes fish flour using Trifolium repense egg albumen powder, can not only
Fish flour usage amount is enough reduced, aquaculture cost is reduced, moreover it is possible to the conditioning and regulation to Fish physiological function is realized, is increased cultivation and is produced
Amount.The present invention also has greatly breakthrough in preparation technology, relative to traditional technology of preparing, it is an advantage of the current invention that first
By ultrasonic-leaching, the cell wall structure of white clover leaves is destroyed, promote the dissolution of nutrient substance in cell, improve
The extraction efficiency and yield of leaf protein, realizes the first extraction of leaf protein by this method；Again by cellulase, pectase and
Protease cooperation is digested to Trifolium repense, realizes the second extraction of leaf protein, is extracted by priority twice, is substantially increased
The extraction efficiency and yield of leaf protein, reduces the production cost of leaf protein；By Folium Isatidiss, Flos Lonicerae, Fructus Forsythiae, Herba Andrographis
Add to coordinate to realize and leaf protein is modified, the immune function of Carassius auratuss can be strengthened, strengthened its disease-resistant, anti-stress ability, carried
High premunition, further improves growth, the production performance of aquatic fish, improves the economic benefit of cultivation.
Specific embodiment
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the present invention, rather than the embodiment of whole, and based on the embodiment in the present invention, this area is common
All other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection
Enclose.
A kind of summer crucian cultivation heat-clearing and toxic substances removing Trifolium repense albumen powder feedstuff, by following weight portion（kg）Raw material system
Into：Trifolium repense 800, cellulase 0.15, pectase 0.12, proteinase-10 .1, Folium Isatidiss 1.3, Flos Lonicerae 1.6, Fructus Forsythiae 1.2, wear
Heart lotus 0.4, raspberry juice 0.5, hydrolysis of chicken meat thing 3, gluten meal 17, blue algae fermentation thing 3, cherry blossom 1, Rhizoma Curcumae 0.7, mushroom powder 14,
Chicken rice noodle roll 7, gutweed vegetable powder 2, Semen arachidis hypogaeae dregs 34, yeast culture 3, Chinese chive juice 2.
Described summer crucian cultivation heat-clearing and toxic substances removing Trifolium repense albumen powder feedstuff, its preparation method are as follows：
（1）The white clover leaves of fresh, no disease and pests harm are gathered, shearing crushing is carried out after cleaning up, by the Trifolium repense after crushing
Mixing according to the ratio that weight compares 1: 10 with water carries out rotation beating at a high speed, arranges beater rotating speed 1800r/min, by what is obtained
Homogenate is heated to 52 DEG C, then using ultrasonic-leaching, setting ultrasonic frequency is 3000KHz, extraction time is 20min, is surpassed
Solid-liquid separation is carried out to homogenate after sound wave extraction, leaching leaf juice is concentrated and is spray-dried, and obtains the first extract powder；
（2）By step（1）Leaf slag after solid-liquid separation is put and is dried in the shade at room temperature, carries out micronizing, 200 mesh of smashing fineness, so
After the regulation that adds water make its water content reach 15%, the extruding puffing under the conditions of 56 DEG C of extrusion temperature, screw speed 320r/min will
The water of 8 times of the Trifolium repense micropowder after expanded and its weight portion is mixed, and is put in enzymatic vessel, keeps tank temperature to be 43 DEG C, then
Cellulase, pectase and protease stirring and evenly mixing are added in tank, the stirring enzymolysis 18h under the conditions of 70r/min, after enzymolysis
Reactant liquor be centrifuged supernatant, by supernatant concentration and be spray-dried, obtain the second extract powder；
（3）Add the water of 5 times of weight portions persistently to decoct 1h after Folium Isatidiss, Flos Lonicerae, Fructus Forsythiae, Herba Andrographis are pulverized and mixed, take pan-fried
Boil liquid and raspberry juice, hydrolysis of chicken meat thing stirring and evenly mixing, are incubated 3h in being put into 84 DEG C of couveuse, take out, and send out with gluten meal, cyanophyceae
The mixing of ferment thing is mixed thoroughly, heat aging 20min, obtains modified adjuvant；
（4）Cherry blossom, Rhizoma Curcumae are decocted into 50min with the water heating of 3 times of weight portions, then step are added to the inside（1）, step
（2）Extract powder and step（3）The mixing of modified adjuvant, keep water content to be 32%, 58 DEG C of insulation reaction 40min are modified
Trifolium repense egg albumen powder；
（5）5 times of water of mushroom powder and its weight portion is mixed and is put in pot, decocted boiling, add chicken rice noodle roll, gutweed vegetable powder and mix
Even, stewing 12min is taken out, and mixes with Semen arachidis hypogaeae dregs, steams 40min, places into
DEG C, expanded time 2s obtains modified and reinforced dose；
（6）By step（4）、（5）Obtained material is mixed, and is added the water stirring and evenly mixing of 12 times of gross weight, is subsequently adding
To in reactor, the temperature for arranging reactor is 72 DEG C, speed of agitator is 130r/min, insulated and stirred 2h, then other are remaining
Raw material is added, and is risen high-temperature to 94 DEG C of insulation reaction 12min, is cooled the temperature to rapidly 18 DEG C again afterwards, using centrifuge to anti-
By supernatant concentration and it is spray-dried after answering liquid centrifugation remove impurity, obtains final products.
Culture experiment is done using feedstuff of the present invention, matched group carries out feeding using common daily ration, and test group is using addition 10%
The common daily ration of feedstuff of the present invention carries out feeding, and in addition, other external conditions are identical.Test result indicate that, test
The speed of growth of group fish is significantly faster than that matched group, and disease resistance is higher, and survival rate is greatly improved, and cultured output is high, fish development shape
State is good.
The ultimate principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel it should be appreciated that the present invention is not restricted to the described embodiments, the simply explanation described in above-described embodiment and description this
The principle of invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, these changes
Change and improvement is both fallen within scope of the claimed invention.The claimed scope of the invention by appending claims and its
Equivalent thereof.
Claims (2)
1. a kind of summer crucian cultivation heat-clearing and toxic substances removing Trifolium repense albumen powder feedstuff, it is characterised in that by the original of following weight portion
Material is made：Trifolium repense 800-850, cellulase 0.15-0.18, pectase 0.12-0.15, proteinase-10 .1-0.12, Folium Isatidiss
1.3-1.5, Flos Lonicerae 1.6-1.8, Fructus Forsythiae 1.2-1.4, Herba Andrographis 0.4-0.6, raspberry juice 0.5-0.7, hydrolysis of chicken meat thing 3-4,
Gluten meal 17-21, blue algae fermentation thing 3-4, cherry blossom 1-1.2, Rhizoma Curcumae 0.7-0.8, mushroom powder 14-16, chicken rice noodle roll 7-8, gutweed
Vegetable powder 2-3, Semen arachidis hypogaeae dregs 34-37, yeast culture 3-5, Chinese chive juice 2-3.
2. summer crucian cultivation according to claim 1 heat-clearing and toxic substances removing Trifolium repense albumen powder feedstuff, its preparation method is such as
Under：
（1）The white clover leaves of fresh, no disease and pests harm are gathered, shearing crushing is carried out after cleaning up, by the Trifolium repense after crushing
Mixing according to the ratio that weight compares 1: 10-15 with water carries out rotation beating at a high speed, arranges beater rotating speed 1800-2400r/min,
The homogenate for obtaining is heated to into 52-56 DEG C, then using ultrasonic-leaching, setting ultrasonic frequency is 3000-5000KHz, leaching
The time is carried for 20-25min, solid-liquid separation after ultrasonic-leaching, is carried out to homogenate, leaching leaf juice is concentrated and is spray-dried,
Obtain the first extract powder；
（2）By step（1）Leaf slag after solid-liquid separation is put and is dried in the shade at room temperature, carries out micronizing, smashing fineness 200-300
Mesh, the regulation that then adds water make its water content reach 15-18%, in 56-58 DEG C of extrusion temperature, screw speed 320-350r/min bars
Extruding puffing under part, will be expanded after the water of 8-10 times of Trifolium repense micropowder and its weight portion mixed, put in enzymatic vessel,
Keep tank temperature to be 43-46 DEG C, then cellulase, pectase and protease stirring and evenly mixing are added in tank, in 70-90r/min bars
Under part, stirring enzymolysis 18-20h, is centrifuged supernatant to the reactant liquor after enzymolysis, by supernatant concentration and is spray-dried,
Obtain the second extract powder；
（3）The water of 5-6 times of weight portion is added persistently to decoct 1-2h after Folium Isatidiss, Flos Lonicerae, Fructus Forsythiae, Herba Andrographis are pulverized and mixed,
Decoction liquor and raspberry juice, hydrolysis of chicken meat thing stirring and evenly mixing are taken, 3-4h in being put into 84-86 DEG C of couveuse, is incubated, is taken out, with Wheat Gluten
Powder, the mixing of blue algae fermentation thing are mixed thoroughly, and heat aging 20-30min obtains modified adjuvant；
（4）Cherry blossom, Rhizoma Curcumae are decocted into 50-60min with the water heating of 3-4 times of weight portion, then step are added to the inside（1）、
Step（2）Extract powder and step（3）The mixing of modified adjuvant, holdings water content is 32-36%, 58-62 DEG C of insulation reaction 40-
50min, obtains modified Trifolium repense egg albumen powder；
（5）5-8 times of water of mushroom powder and its weight portion is mixed and is put in pot, decocted boiling, add chicken rice noodle roll, gutweed vegetable powder
Mix thoroughly, stewing 12-15min take out, mix with Semen arachidis hypogaeae dregs, steam 40-50min, place into carry out in bulking machine it is expanded, setting it is expanded
Temperature 130-150 DEG C, expanded time 2-3s, obtains modified and reinforced dose；
（6）By step（4）、（5）Obtained material is mixed, and adds the water stirring and evenly mixing of gross weight 12-15 times, Ran Houjia
Enter in reactor, arrange reactor temperature be 72-75 DEG C, speed of agitator be 130-150r/min, insulated and stirred 2-3h, then
Other surplus stocks are added, and high-temperature are risen to 94-98 DEG C of insulation reaction 12-15min, are cooled the temperature to rapidly 18- afterwards again
22 DEG C, reactant liquor is centrifuged supernatant concentration after remove impurity and is spray-dried using centrifuge, obtain final products.
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| CN201610984179.8A CN106578661A (en) | 2016-11-09 | 2016-11-09 | Heat clearing and detoxification trifolium repens l protein powder feed for crucian breeding in summer, and preparation method thereof |
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| CN201610984179.8A CN106578661A (en) | 2016-11-09 | 2016-11-09 | Heat clearing and detoxification trifolium repens l protein powder feed for crucian breeding in summer, and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110959777A (en) * | 2019-12-23 | 2020-04-07 | 福建上一饲料有限公司 | Formula and production process of aquatic animal immunopotentiator |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1618312A (en) * | 2004-11-29 | 2005-05-25 | 朱超 | Forage, and prodn, method therefor |
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2016
- 2016-11-09 CN CN201610984179.8A patent/CN106578661A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1618312A (en) * | 2004-11-29 | 2005-05-25 | 朱超 | Forage, and prodn, method therefor |
Non-Patent Citations (1)
| Title |
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| 玉柱，等: "不同添加剂对白三叶青贮发酵品质与体外消化率的影响", 《中国农业科技导报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110959777A (en) * | 2019-12-23 | 2020-04-07 | 福建上一饲料有限公司 | Formula and production process of aquatic animal immunopotentiator |
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Application publication date: 20170426 |