CN106563172A - Preparation method for preparing polylactic acid tissue engineering scaffold - Google Patents
Preparation method for preparing polylactic acid tissue engineering scaffold Download PDFInfo
- Publication number
- CN106563172A CN106563172A CN201610970143.4A CN201610970143A CN106563172A CN 106563172 A CN106563172 A CN 106563172A CN 201610970143 A CN201610970143 A CN 201610970143A CN 106563172 A CN106563172 A CN 106563172A
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- Prior art keywords
- polylactic acid
- tissue engineering
- engineering scaffold
- preparation
- engineering bracket
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/025—Other specific inorganic materials not covered by A61L27/04 - A61L27/12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention relates to a preparation method for preparing a polylactic acid tissue engineering scaffold. According to the method, 25-40% of polylactic acid, 30-45% of polyoxymethylene and 25-40% of sodium bicarbonate are mixed up to prepare a mixed solution and then the mixed solution is subjected to spinning to obtain polylactic acid electrospun nanofibers. The nanofibers are processed in supercritical carbon dioxide. Through controlling the processing pressure and the processing temperature, an intermediate product is obtained. The obtained intermediate product is subjected to leaching in the circulating water bath and then drying in vacuum, and then the tissue engineering scaffold is obtained. The obtained tissue engineering scaffold is made of porous materials, wherein the porosity of the tissue engineering scaffold is 68 to 74.0% and the cell size of the tissue engineering scaffold is 20 to 200 microns. The dimension of the inner communicating channel of the tissue engineering scaffold is about 20 to 200 microns. According to the technical scheme of the preparation method in the invention, fibers can be cross-linked and fused at a relatively low temperature without any solvent or cross-linking agent. Meanwhile, the generation of cytotoxicity is avoided.
Description
Technical field
The invention belongs to technical field of biomedical materials, and in particular to a kind of preparation side of polylactic acid tissue engineering bracket
Method.
Background technology
Tissue engineering bracket material is that biological internal material can be combined and can be implanted into tissue biopsy cell, and it can be thin
Born of the same parents provide the place for obtaining nutrition, gas exchange, discharged waste and growth promoter, are also to form new with form and function
Tissue, the material base of organ.
For tissue engineering bracket, tissue engineering bracket material is the key of tissue defect regeneration.Organizational project
The porosity and pore size of frame material is the key factor for affecting tissue engineering bracket material performance.Except requiring organizational project
Timbering material has beyond higher porosity, also has strict demand to pore size, and hole is too little, and cell cannot be introduced into hole
Or block cell breeding and amplification；Hole is too big, and cell adhesion is not lived, and loses the effect as support.
The conventional method for preparing tissue engineering bracket has phase separation method, solution-cast-particle lavage, fiber bonding method
And gas foaming method etc..But, prior art is made crosslinked together between polymer fiber using cross-linking agent or solvent etc..It is existing
There are the solvent adopted in technology and cross-linking agent etc. to be very difficult to except clean, so as to produce when in use in tissue engineering bracket material
Cytotoxicity, causes surrounding tissue inflammatory reaction, destroys the biological activity of cell and tissue, affects neoblastic formation and reparation
Effect.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of polylactic acid tissue engineering bracket, is can to meet cell growth to want
The porous material asked, the method prepares tissue engineering bracket without the need for solvent and cross-linking agent.
The present invention is achieved by the following technical solutions：
(1) by macromolecule raw material polylactic acid, polyethylene glycol oxide and porogen sodium bicarbonate according to following percents by volume
The mixed solution being formulated：
Proportioning：Polylactic acid (graininess) 25～40%,
Polyethylene glycol oxide (powder) 30～45%,
Sodium bicarbonate (powder) 25～40%,
Wherein, polylactic acid will ensure 1 with the percent by volume of sodium bicarbonate:1；
(2) the raw material mixed liquor of step (1) is carried out into spinning, obtains polylactic acid nano fiber；
(3) polylactic acid nano fiber of step (2) is put in tissue engineering bracket mould, then will be equipped with nanofiber
Tissue engineering bracket mould be put into pressure be 12MPa～25MPa supercritical CO2Middle process, control process temperature be 40～
150 DEG C, CO is discharged in then decompression2, intermediate products are obtained, by resulting intermediate products, vacuum is dried after leaching in circulator bath
It is dry, you can obtain porosity up to 68～74.0%, abscess-size be 20～200 microns, inside be interconnected channel size for 20
The polylactic acid tissue engineering bracket of～200 microns of porous material.
Preferably, the supercritical CO2Middle treatment temperature is 35～200 DEG C.
Preferably, the supercritical CO2Middle process time is 0.5～48 hour.
Preferably, the supercritical CO2Middle processing pressure is 10MPa～30MPa.
Preferably, the discharge CO2Temperature be 4～350 DEG C.
Preferably, the discharge CO2Time be 5 seconds～20 minutes.
It can be seen from above-mentioned technical scheme that, the invention provides a kind of preparation side of polylactic acid tissue engineering bracket
Method, adopts polylactic acid nano fiber in supercritical CO2The method of middle process, by control process pressure and treatment temperature, obtains
To tissue engineering bracket.Due to supercritical CO2With certain solvent property, produce on the surface of degradable polymer fiber micro-
Thawing is acted on, when CO is discharged in decompression2Afterwards, surface melts partially cured crosslinked together between the polylactic acid nano fiber of contact,
So as to form the tissue engineering bracket with excellent mechanical performances.
The present invention has the advantages that：
1. the preparation method that the present invention is provided need not can complete fine under lower temperature state using solvent and cross-linking agent etc.
Crosslinking between dimension and fusion, will not produce cytotoxicity.
2. the physical blowing agent (supercritical carbon dioxide) for not only having microporous foam formation in foaming process produces foaming work
With, also there is CBA sodium bicarbonate to decompose the foaming effect of gained gas simultaneously, this physical chemistry combines foaming method
So that the polylactic acid tissue engineering bracket for finally giving meets the requirement of Growth of Biologic Cell.
Specific embodiment
The present invention is further illustrated with reference to embodiment.
Embodiment 1
A kind of preparation method of polylactic acid tissue engineering bracket is realized by procedure below：
(1) by polylactic acid (graininess), polyethylene glycol oxide (powder), sodium bicarbonate (powder) according to volume ratio 1：1：
1 is configured to mixed solution；
(2) the raw material mixed liquor of step (1) is carried out into spinning, obtains polylactic acid nano fiber；
(3) polylactic acid nano fiber of step (2) is put in tissue engineering bracket mould, then will be equipped with nanofiber
Tissue engineering bracket mould be put into pressure be 20MPa supercritical CO2Middle process, control process temperature is 55 DEG C, processes 1 little
When, CO is discharged in then decompression2, control time is 1 minute, and temperature control during decompression is obtained intermediate products, by gained at 70 DEG C
To intermediate products in circulator bath vacuum drying after leaching, you can obtain porosity up to 68.2%, abscess-size be 20～
300 microns, inside be interconnected the polylactic acid tissue engineering bracket of porous material that channel size is 20～200 microns, i.e.,
Communicating passage is suitable with abscess-size between abscess, beneficial to cell on support apposition growth, while it is also ensured that cell migration
Into inside timbering material.
Embodiment 2
By polylactic acid (graininess), polyethylene glycol oxide (powder), sodium bicarbonate (powder) according to volume ratio 3：2：3 match somebody with somebody
System, step same as Example 1, parameter is adjusted, wherein supercritical CO2Middle processing controls temperature is 55 DEG C, processes 1 little
When, CO is discharged in then decompression2, control time be 1 minute, temperature control during decompression at 70 DEG C, what prepared inside was interconnected
Porous material polylactic acid tissue engineering bracket, the porosity of gained porous material is 73.8%, abscess-size is 20 microns-
250 microns, inside is interconnected channel size for 20 microns -200 microns.
Although above-mentioned be described to the specific embodiment of the present invention in conjunction with the embodiments, not to present invention protection
The restriction of scope, one of ordinary skill in the art should be understood that on the basis of technical scheme, those skilled in the art
The various modifications made by creative work need not be paid or deformation are still within protection scope of the present invention.
Claims (2)
1. a kind of preparation method of polylactic acid tissue engineering bracket, is characterized in that, step is as follows：
(1) polylactic acid, polyethylene glycol oxide and sodium bicarbonate are formulated into mixed solution according to following percents by volume：
Proportioning：Polylactic acid 25～40%,
Polyethylene glycol oxide 30～45%,
Sodium bicarbonate 25～40%；
(2) the raw material mixed liquor of step (1) is carried out into spinning, obtains polylactic acid nano fiber；
(3) polylactic acid nano fiber of step (2) is put into the group that nanofiber is then will be equipped with tissue engineering bracket mould
Weaver's engineering support mould is put into the supercritical CO that pressure is 12MPa～25MPa2Middle process, control process temperature is 40～150
DEG C, CO is discharged in then decompression2, intermediate products are obtained, by resulting intermediate products in circulator bath vacuum drying after leaching,
Be obtained porosity up to 68～74.0%, abscess-size be 20～200 microns, inside be interconnected channel size for 20～
The polylactic acid tissue engineering bracket of 200 microns of porous material.
2. a kind of preparation method of polylactic acid tissue engineering bracket according to claim 1, is characterized in that, the supercritical
CO2Middle process time is 0.5～48 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610970143.4A CN106563172A (en) | 2016-11-07 | 2016-11-07 | Preparation method for preparing polylactic acid tissue engineering scaffold |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610970143.4A CN106563172A (en) | 2016-11-07 | 2016-11-07 | Preparation method for preparing polylactic acid tissue engineering scaffold |
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| CN106563172A true CN106563172A (en) | 2017-04-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610970143.4A Pending CN106563172A (en) | 2016-11-07 | 2016-11-07 | Preparation method for preparing polylactic acid tissue engineering scaffold |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3684726A4 (en) * | 2017-09-19 | 2021-06-30 | Board of Regents of the University of Nebraska | Nanofiber structures and methods of use thereof |
| US11738116B2 (en) | 2017-06-09 | 2023-08-29 | Board Of Regents Of The University Of Nebraska | Expanded nanofiber structures comprising electrospun nanofibers and a plurality of holes and methods of making and use thereof |
| US11951227B2 (en) | 2016-09-28 | 2024-04-09 | Board Of Regents Of The University Of Nebraska | Nanofiber structures and methods of use thereof |
-
2016
- 2016-11-07 CN CN201610970143.4A patent/CN106563172A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11951227B2 (en) | 2016-09-28 | 2024-04-09 | Board Of Regents Of The University Of Nebraska | Nanofiber structures and methods of use thereof |
| US11738116B2 (en) | 2017-06-09 | 2023-08-29 | Board Of Regents Of The University Of Nebraska | Expanded nanofiber structures comprising electrospun nanofibers and a plurality of holes and methods of making and use thereof |
| EP3684726A4 (en) * | 2017-09-19 | 2021-06-30 | Board of Regents of the University of Nebraska | Nanofiber structures and methods of use thereof |
| US11427936B2 (en) | 2017-09-19 | 2022-08-30 | Board Of Regents Of The University Of Nebraska | Methods for producing a nanofiber or microfiber structure |
| US11946164B2 (en) | 2017-09-19 | 2024-04-02 | Board Of Regents Of The University Of Nebraska | Nanofiber structures and methods of use thereof |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170419 |
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| WD01 | Invention patent application deemed withdrawn after publication |