CN106421898A - Preparation method of polylactide coglycolide scaffolds for tissue engineering - Google Patents

Preparation method of polylactide coglycolide scaffolds for tissue engineering Download PDF

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Publication number
CN106421898A
CN106421898A CN201610970144.9A CN201610970144A CN106421898A CN 106421898 A CN106421898 A CN 106421898A CN 201610970144 A CN201610970144 A CN 201610970144A CN 106421898 A CN106421898 A CN 106421898A
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China
Prior art keywords
polylactide
tissue engineering
engineering bracket
preparation
supercritical
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CN201610970144.9A
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Chinese (zh)
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王淑芳
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Individual
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The invention relates to a preparation method of polylactide coglycolide scaffolds for tissue engineering. The method comprises the steps of mixing 25-40% of polylactide coglycolide, 30-40% of polyvinyl alcohol and 25-40% of sodium hydrogen carbonate into solution, conducting spinning to acquire polylactide coglycolide nano-fiber, conducting the processing of the nano-fiber in supercritical CO2, through controlling the processing pressure and processing temperature, the middle product can be acquired, leaching the middle acquired product in circulating bath, drying the middle product to acquire the tissue engineering scaffolds which is made of a porous material with 68-74.0% porosity, 20-200 micrometers abscess size, and is internally communicated by communication channels with a size of 20-200 micrometers. The method can achieve the cross-linking and fusion between fibers under low temperature without the addition of solvent and cross-linking agent. The method generates no cytotoxicity.

Description

A kind of preparation method of polylactide tissue engineering bracket
Technical field
The invention belongs to technical field of biomedical materials is and in particular to a kind of polylactide tissue engineering bracket Preparation method.
Background technology
Tissue engineering bracket material is to be combined and can implant biological internal material with tissue biopsy cell, and it can be thin Born of the same parents provide and obtain nutrition, the place of gas exchange, discharged waste and growth promoter, are also to be formed new to have form and function Tissue, the material base of organ.
For tissue engineering bracket, tissue engineering bracket material is the key of tissue defect regeneration.Organizational project is propped up The porosity of frame material and pore size are the key factors affecting tissue engineering bracket material performance.Except requiring organizational project Timbering material has beyond higher porosity, also has strict demand to pore size, and hole is too little, and cell cannot be introduced into hole Or block cell breeding and amplification；Hole is too big, and cell adhesion does not live, and loses the effect as support.
The conventional method preparing tissue engineering bracket has phase separation method, solution-cast-particle lavage, fiber bonding method And gas foaming method etc..But, prior art is all made crosslinked together between polymer fiber using cross-linking agent or solvent etc..Existing The solvent adopting in technology and cross-linking agent etc. is had to be very difficult to except clean, thus producing when using in tissue engineering bracket material Cytotoxicity, causes surrounding tissue inflammatory reaction, destroys the biological activity of cell and tissue, and impact is neoblastic to be formed and repair Effect.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of polylactide tissue engineering bracket, thin for meeting The porous material that intracellular growth requires, the method, without solvent and cross-linking agent, prepares tissue engineering bracket.
The present invention is achieved by the following technical solutions：
(1) by macromolecule raw material polylactide, polyvinyl alcohol and porogen sodium bicarbonate according to following volumes The mixed solution that percentage ratio is formulated：
Proportioning：Polylactide (graininess) 25～40%,
Polyvinyl alcohol (powder) 30～45%,
Sodium bicarbonate (powder) 25～40%,
Wherein, polylactide and the percent by volume of sodium bicarbonate will ensure 1:1；
(2) the raw material mixed liquor of step (1) is carried out spinning, obtain polylactide nanofiber；
(3) the polylactide nanofiber of step (2) is put in tissue engineering bracket mould, then will be equipped with The supercritical CO that pressure is 12MPa～25MPa put into by the tissue engineering bracket mould of nanofiber2Middle process, control process temperature Spend for 40～150 DEG C, CO is discharged in then decompression2, prepared intermediate products, the leaching in circulator bath by obtained intermediate products Vacuum drying afterwards, you can obtain porosity and reach that 68～74.0%, abscess-size is 20～200 microns, inside is interconnected passage The polylactide tissue engineering bracket of a size of 20～200 microns of porous material.
Preferably, described supercritical CO2Middle treatment temperature is 35～200 DEG C.
Preferably, described supercritical CO2Middle process time is 0.5～48 hour.
Preferably, described supercritical CO2Middle processing pressure is 10MPa～30MPa.
Preferably, described discharge CO2Temperature be 4～350 DEG C.
Preferably, described discharge CO2Time be 5 seconds～20 minutes.
It can be seen from above-mentioned technical scheme that, the invention provides a kind of polylactide tissue engineering bracket Preparation method, adopts polylactide nanofiber in supercritical CO2The method of middle process, by control process pressure And treatment temperature, obtain tissue engineering bracket.Due to supercritical CO2There is certain solvent property, fine in degradable polymer class The surface of dimension produces micro- thawing effect, when CO is discharged in decompression2Afterwards, between the polylactide nanofiber of contact, surface is melted Change partially cured crosslinked together, thus forming the tissue engineering bracket with excellent mechanical performances.
The present invention has the advantages that：
1. the preparation method that the present invention provides can need not be completed fine under lower temperature state using solvent and cross-linking agent etc. Crosslinking between dimension and fusion, will not produce cytotoxicity.
2. the physical blowing agent (supercritical carbon dioxide) not only having microporous foam formation in foaming process produces foaming work With also there being CBA sodium bicarbonate to decompose the foaming effect of gained gas, this physical chemistry joint foaming method simultaneously So that the polylactide tissue engineering bracket finally giving meets the requirement of Growth of Biologic Cell.
Specific embodiment
Further illustrate the present invention with reference to embodiment.
Embodiment 1
A kind of preparation method of polylactide tissue engineering bracket is realized by procedure below：
(1) by polylactide (graininess), polyvinyl alcohol (powder), sodium bicarbonate (powder) according to volume Ratio 1：1：1 is configured to mixed solution；
(2) the raw material mixed liquor of step (1) is carried out spinning, obtain polylactide nanofiber；
(3) the polylactide nanofiber of step (2) is put in tissue engineering bracket mould, then will be equipped with The supercritical CO that pressure is 20MPa put into by the tissue engineering bracket mould of nanofiber2Middle process, control process temperature is 55 DEG C, process 1 hour, CO is discharged in then decompression2, control time is 1 minute, and temperature control during decompression, at 70 DEG C, is obtained middle Product, by vacuum drying after the leaching in circulator bath of obtained intermediate products, you can obtain porosity and reach 68.2%, abscess A size of 20～300 microns, the polylactide second friendship of the internal porous material that channel size is 20～200 microns that is interconnected Ester tissue engineering bracket, that is, between abscess, communicating passage is suitable with abscess-size, beneficial to cell on support apposition growth, simultaneously Can ensure that cell migration enters inside timbering material.
Embodiment 2
By polylactide (graininess), polyvinyl alcohol (powder), sodium bicarbonate (powder) according to volume ratio 3：2：3 preparations, step same as Example 1, parameter is adjusted, wherein supercritical CO2Middle processing controls temperature is 55 DEG C, Process 1 hour, CO is discharged in then decompression2, control time is 1 minute, and temperature control during decompression, at 70 DEG C, is obtained internal mutual The porous material polylactide tissue engineering bracket of connection, the porosity of gained porous material is 73.8%, bubble A size of 20 microns -250 microns of hole, inside is interconnected channel size for 20 microns -200 microns.
Although above-mentioned be described to the specific embodiment of the present invention in conjunction with the embodiments, not the present invention is protected The restriction of scope, one of ordinary skill in the art should be understood that on the basis of technical scheme, those skilled in the art Do not need to pay the various modifications that creative work can make or deformation still within protection scope of the present invention.

Claims (2)

1. a kind of preparation method of polylactide tissue engineering bracket, is characterized in that, step is as follows：
(1) polylactide, polyvinyl alcohol and sodium bicarbonate are formulated mixing according to following percents by volume molten Liquid：
Proportioning：Polylactide 25～40%,
Polyvinyl alcohol 30～45%,
Sodium bicarbonate 25～40%；
(2) the raw material mixed liquor of step (1) is carried out spinning, obtain polylactide nanofiber；
(3) the polylactide nanofiber of step (2) is put in tissue engineering bracket mould, then will be equipped with nanometer The supercritical CO that pressure is 12MPa～25MPa put into by the tissue engineering bracket mould of fiber2Middle process, control process temperature is 40～150 DEG C, CO is discharged in then decompression2, prepared intermediate products, will be true after the leaching in circulator bath of obtained intermediate products Empty dry, you can obtain porosity and reach that 68～74.0%, abscess-size is 20～200 microns, inside is interconnected channel size Polylactide tissue engineering bracket for 20～200 microns of porous materials.
2. a kind of preparation method of polylactide tissue engineering bracket according to claim 1, is characterized in that, institute State supercritical CO2Middle process time is 0.5～48 hour.
CN201610970144.9A 2016-11-07 2016-11-07 Preparation method of polylactide coglycolide scaffolds for tissue engineering Pending CN106421898A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610970144.9A CN106421898A (en) 2016-11-07 2016-11-07 Preparation method of polylactide coglycolide scaffolds for tissue engineering

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610970144.9A CN106421898A (en) 2016-11-07 2016-11-07 Preparation method of polylactide coglycolide scaffolds for tissue engineering

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Publication Number Publication Date
CN106421898A true CN106421898A (en) 2017-02-22

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3684726A4 (en) * 2017-09-19 2021-06-30 Board of Regents of the University of Nebraska Nanofiber structures and methods of use thereof
US11738116B2 (en) 2017-06-09 2023-08-29 Board Of Regents Of The University Of Nebraska Expanded nanofiber structures comprising electrospun nanofibers and a plurality of holes and methods of making and use thereof
US11951227B2 (en) 2016-09-28 2024-04-09 Board Of Regents Of The University Of Nebraska Nanofiber structures and methods of use thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11951227B2 (en) 2016-09-28 2024-04-09 Board Of Regents Of The University Of Nebraska Nanofiber structures and methods of use thereof
US11738116B2 (en) 2017-06-09 2023-08-29 Board Of Regents Of The University Of Nebraska Expanded nanofiber structures comprising electrospun nanofibers and a plurality of holes and methods of making and use thereof
EP3684726A4 (en) * 2017-09-19 2021-06-30 Board of Regents of the University of Nebraska Nanofiber structures and methods of use thereof
US11427936B2 (en) 2017-09-19 2022-08-30 Board Of Regents Of The University Of Nebraska Methods for producing a nanofiber or microfiber structure
US11946164B2 (en) 2017-09-19 2024-04-02 Board Of Regents Of The University Of Nebraska Nanofiber structures and methods of use thereof

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