CN106562123A - Freshwater fish microbial preparation composition and applications thereof - Google Patents
Freshwater fish microbial preparation composition and applications thereof Download PDFInfo
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- CN106562123A CN106562123A CN201610968303.1A CN201610968303A CN106562123A CN 106562123 A CN106562123 A CN 106562123A CN 201610968303 A CN201610968303 A CN 201610968303A CN 106562123 A CN106562123 A CN 106562123A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses a freshwater fish microbial preparation composition, and applications thereof. The freshwater fish microbial preparation composition is composed of, by weight, 10 to 20 parts of Fu tea probiotic powder, 10 to 20 parts of bifidobacterium powder, 20 to 40 parts of sweet potato leaf powder, 5 to 10 parts of medicated leaven, 1 to 5 parts of magnesium citrate, 1 to 5 parts of taurine, 0.01 to 0.05 part of tryptophan, 5 to 15 parts of fructooligosaccharide, 5 to 15 parts of galactooligosaccharides, and 10 to 30 parts of white carbon black. The freshwater fish microbial preparation composition is capable of improving intestinal health of freshwater fish fundamentally, and increasing weight gain rate and food intake amount.
Description
Technical field
The present invention relates to a kind of microorganism formulation, more particularly, to a kind of fresh-water fishes microorganism formulation compositionss and its use
On the way.
Background technology
With developing rapidly for fresh water intensive culture pattern, breeding production unit in order to pursue high economic benefit, in a large number
Using the mixed feed of cheap nutrient imbalance, chemical growth promoter and antibioticses immunity additive, to freshwater fish intestinal
Road biological community structure causes heavy damage.Do so not only there is no the cultivated animals speed of growth faster, on the contrary
Can be because intestinal microflora is unbalance to reduce its absorption to nutrient substance.Meanwhile, the decline of advantage beneficial flora causes cause of disease
Microorganism is easier to be colonized on intestinal wall, causes susceptibility of the body to pathogenic microorganism to improve.
Freshwater fish intestinal microbial population balance is maintained for improving its growth performance, efficiency of feed utilization, immunity and premunition
There are vital effect, such as prior art Authorization Notice No.：The Chinese invention patent of CN103875920B, the disclosure of the invention
A kind of Radix Et Rhizoma Rhei fish feed additive with intestinal tract protection effect and its preparation method and application, feature be by following raw material and
Its parts by weight composition：Oil of rosemary 18-25, Oleum menthae 16-26, tangerine oil 15-20, calcium alginate 20-30, arginine
10-20, glutamine 5-10, its preparation process is to take oil of rosemary, Oleum menthae, tangerine oil, calcium alginate, arginine, paddy ammonia
Amide, after above-mentioned parts by weight mix homogeneously, is spray-dried, and advantage is that the Radix Et Rhizoma Rhei fish feed additive adds in feedstuff
Plus quality is only 0.07～0.12%, you can protect Carnis Pseudosciaenae intestinal fine hair from the damage of antinutritional factor in plant protein source,
With the growth for remarkably promoting Carnis Pseudosciaenae, rate of body weight gain can improve more than 8%, and food ration improves more than 10%, and protein digestibility is carried
It is high by more than 13%, although rate of body weight gain, food ration and digestibility all increase, but it can not ask from the basic intestinal health that solves
Topic, while rate of body weight gain, food ration and digestibility can also be improved again.
The content of the invention
The present invention provides a kind of from basic solution intestinal health to solve above-mentioned technical problem, improves rate of body weight gain and food ration
A kind of fresh-water fishes microorganism formulation compositionss and application thereof.
The present invention solve the technical scheme that adopted of above-mentioned technical problem for：Fresh-water fishes microorganism formulation compositionss and its use
On the way, wherein：
The separation of strain:Precision weighs 1.00g tea samples, adds sterilized water to 500mL, soaks 5h.10 μ are taken with aseptic liquid-transfering gun
In L injection plating mediums, shake up, be placed in culture in 28.5 DEG C of constant incubators, bacterium colony covers with whole flat board, root after 3～5d
According to the morphological characteristic of just point bacterium colony, with a small amount of mycelium of each bacterium colony of Inoculating needle picking, preprepared culture medium is seeded in
It is interior, after bacterium colony grows, screened and purification according to each bacterium colony feature for growing, so repeatedly, until the growth conditions of bacterium colony
It is considered as the bacterium colony of single culture when showing consistent with morphological characteristic.Then with Eurotium Cristatum as dominant bacteria, separate one by one, obtain Fu
Tea probiotic bacteria, is made into afterwards FUCHA probiotic bacteria mycopowder.
Buy in Bifidobacteria powder shop；
Microorganism formulation compositionss are made up of following raw material and its parts by weight：10～20 parts of FUCHA probiotic bacteria mycopowder, bifid bar
10～20 parts of bacterium mycopowder, 20～40 parts of Folium Ipomoea powder, 5～10 parts of Massa Medicata Fermentata, 1～5 part of magnesium citrate, 1～5 part of taurine, color
0.01～0.05 part of propylhomoserin, 5～15 parts of fruit oligose, 5～15 parts of galactooligosacchari(es, 10～30 parts of white carbon, because the mechanism of action is failed to understand
Really or because of synergism, can effectively suppress field planting of the microorganism in intestinal, quickly repair injured intestinal mucosa, improve intestinal and be good for
Health, promotes to selecting property the propagation of corresponding probiotic bacteria, and from basic intestinal health is solved the problems, such as, and improves survival rate, while promoting light
Water fish intestinal is digested and assimilated and digested to nutrient substance, with excellent antibacterial ability, so as to improve rate of body weight gain and food ration,
Because the mechanism of action is indefinite, test still to be tested.
Preferably, it is characterised in that：Microorganism formulation compositionss are made up of following raw material and its parts by weight：FUCHA is prebiotic
15 parts of bacterium mycopowder, 15 parts of Bifidobacteria powder, 30 parts of Folium Ipomoea powder, 10 parts of Massa Medicata Fermentata, 2 parts of magnesium citrate, 4 parts of taurine, color
0.01 part of propylhomoserin, 10 parts of fruit oligose, 10 parts of galactooligosacchari(es, 20 parts of white carbon.
Or, microorganism formulation compositionss are made up of following raw material and its parts by weight：It is 10 parts of FUCHA probiotic bacteria mycopowder, double
20 parts of discrimination bacillus mycopowder, 20 parts of Folium Ipomoea powder, 5 parts of Massa Medicata Fermentata, 1 part of magnesium citrate, 1 part of taurine, 0.03 part of tryptophan, fruit are few
5 parts of sugar, 5 parts of galactooligosacchari(es, 10 parts of white carbon.
Or, microorganism formulation compositionss are made up of following raw material and its parts by weight：It is 20 parts of FUCHA probiotic bacteria mycopowder, double
10 parts of discrimination bacillus mycopowder, 40 parts of Folium Ipomoea powder, 7 parts of Massa Medicata Fermentata, 5 parts of magnesium citrate, 3 parts of taurine, 0.05 part of tryptophan, fruit are few
15 parts of sugar, 15 parts of galactooligosacchari(es, 30 parts of white carbon.
Microorganism formulation compositionss are used to prepare the use of the microorganism formulation compositionss that can improve fresh-water fishes intestinal health
On the way.
Addition of the microorganism formulation compositionss in fresh water fish feed is 5～10%.
Microorganism formulation compositionss outside 5～8% dosage by being sprayed in cultured freshwater fish feedstuff.
Microorganism formulation compositionss are mixed in cultured freshwater fish feedstuff by 8～10% dose uniformity.
Compared with prior art, it is an advantage of the current invention that：The present invention can effectively suppress field planting of the microorganism in intestinal,
It is quick to repair injured intestinal mucosa, improve intestinal health, promote to selecting property the propagation of corresponding probiotic bacteria, solve intestinal health from basic
Problem, improves survival rate, while promote fresh-water fishes intestinal digesting and assimilating and digest to nutrient substance, with excellent antibacterial energy
Power, so as to improve rate of body weight gain and food ration.
Specific embodiment
The present invention is described in further detail with reference to embodiments.
Embodiment 1：
The separation of strain:Precision weighs 1.00g tea samples, adds sterilized water to 500mL, soaks 5h.10 μ are taken with aseptic liquid-transfering gun
In L injection plating mediums, shake up, be placed in culture in 28.5 DEG C of constant incubators, bacterium colony covers with whole flat board, root after 3～5d
According to the morphological characteristic of just point bacterium colony, with a small amount of mycelium of each bacterium colony of Inoculating needle picking, preprepared culture medium is seeded in
It is interior, after bacterium colony grows, screened and purification according to each bacterium colony feature for growing, so repeatedly, until the growth conditions of bacterium colony
It is considered as the bacterium colony of single culture when showing consistent with morphological characteristic.Then with Eurotium Cristatum as dominant bacteria, separate one by one, obtain Fu
Tea probiotic bacteria.FUCHA probiotic bacteria mycopowder is made into afterwards
Bifidobacteria powder shop is bought, and wherein FUCHA probiotic bacteria is formed in ripe tea sweat, and dominant bacteria is Eurotium Cristatum (hat
Prominent bulk bacteria), it is possible to increase fresh-water fishes intestinal is digested and assimilated to nutrient substance, and flora is colonized on fish intestines, can be pressed down
The breeding of pathogenic microorganism processed, quickly repairs injured intestinal mucosa, improves intestinal health；Bacillus bifiduss are intestinal dominant microflora, its
Quantity number be weigh intestinal health leading indicator, maintain intestinal in bifidobacteria advantage, can suppress pathogenic
Field planting of the microorganism in intestinal；
Microorganism formulation compositionss are made up of following raw material and its parts by weight：10～20 parts of FUCHA probiotic bacteria mycopowder, bifid bar
10～20 parts of bacterium mycopowder, 20～40 parts of Folium Ipomoea powder, 5～10 parts of Massa Medicata Fermentata, 1～5 part of magnesium citrate, 1～5 part of taurine, color
0.01～0.05 part of propylhomoserin, 5～15 parts of fruit oligose, 5～15 parts of galactooligosacchari(es, 10～30 parts of white carbon, because of synergism, can have
Effect suppresses field planting of the microorganism in intestinal, quickly repairs injured intestinal mucosa, improves intestinal health, promotes to selecting property corresponding prebiotic
The propagation of bacterium, from intestinal health is solved the problems, such as at all, improves survival rate, while promoting digestion of the fresh-water fishes intestinal to nutrient substance
Absorb and digest, with excellent antibacterial ability, so as to improve rate of body weight gain and food ration, because the mechanism of action is still not clear, experiment
It is still to be tested, contrast experiment set by the present inventor is the following is, if matched group（15 parts of FUCHA probiotic bacteria mycopowder, Bifidobacteria powder
15 parts, 30 parts of Folium Ipomoea powder, 10 parts of fruit oligose, 10 parts of galactooligosacchari(es, 20 parts of white carbon）；Embodiment group（FUCHA probiotics bacterial
15 parts of powder, 15 parts of Bifidobacteria powder, 30 parts of Folium Ipomoea powder, 10 parts of Massa Medicata Fermentata, 2 parts of magnesium citrate, 4 parts of taurine, tryptophan
0.01 part, 10 parts of fruit oligose, 10 parts of galactooligosacchari(es, 20 parts of white carbon）, in the case where other conditions are consistent, items can be improved
Comparing result such as following table：
| Rate of body weight gain（%） | Food ration（%） | Survival rate（%） | |
| Matched group | 8.87 | 11.8 | 64.89 |
| Embodiment group | 14.89 | 17.9 | 78.65 |
Learn from upper table, the present embodiment can improve more than 14.89 in rate of body weight gain, and food ration can improve more than 17.9, and survival rate is
78.65, all higher than matched group.
Preferably, it is characterised in that：Microorganism formulation compositionss are made up of following raw material and its parts by weight：FUCHA is prebiotic
15 parts of bacterium mycopowder, 15 parts of Bifidobacteria powder, 30 parts of Folium Ipomoea powder, 10 parts of Massa Medicata Fermentata, 2 parts of magnesium citrate, 4 parts of taurine, color
0.01 part of propylhomoserin, 10 parts of fruit oligose, 10 parts of galactooligosacchari(es, 20 parts of white carbon.
Or, microorganism formulation compositionss are made up of following raw material and its parts by weight：It is 10 parts of FUCHA probiotic bacteria mycopowder, double
20 parts of discrimination bacillus mycopowder, 20 parts of Folium Ipomoea powder, 5 parts of Massa Medicata Fermentata, 1 part of magnesium citrate, 1 part of taurine, 0.03 part of tryptophan, fruit are few
5 parts of sugar, 5 parts of galactooligosacchari(es, 10 parts of white carbon.
Or, microorganism formulation compositionss are made up of following raw material and its parts by weight：It is 20 parts of FUCHA probiotic bacteria mycopowder, double
10 parts of discrimination bacillus mycopowder, 40 parts of Folium Ipomoea powder, 7 parts of Massa Medicata Fermentata, 5 parts of magnesium citrate, 3 parts of taurine, 0.05 part of tryptophan, fruit are few
15 parts of sugar, 15 parts of galactooligosacchari(es, 30 parts of white carbon.
Microorganism formulation compositionss are used to prepare the use of the microorganism formulation compositionss that can improve fresh-water fishes intestinal health
On the way.
Addition of the microorganism formulation compositionss in fresh water fish feed is 5～10%.
Microorganism formulation compositionss outside 5～8% dosage by being sprayed in cultured freshwater fish feedstuff.
Microorganism formulation compositionss are mixed in cultured freshwater fish feedstuff by 8～10% dose uniformity.
Embodiment 2：
The separation of strain:Precision weighs 1.00g tea samples, adds sterilized water to 500mL, soaks 5h.10 μ are taken with aseptic liquid-transfering gun
In L injection plating mediums, shake up, be placed in culture in 28.5 DEG C of constant incubators, bacterium colony covers with whole flat board, root after 3～5d
According to the morphological characteristic of just point bacterium colony, with a small amount of mycelium of each bacterium colony of Inoculating needle picking, preprepared culture medium is seeded in
It is interior, after bacterium colony grows, screened and purification according to each bacterium colony feature for growing, so repeatedly, until the growth conditions of bacterium colony
It is considered as the bacterium colony of single culture when showing consistent with morphological characteristic.Then with Eurotium Cristatum as dominant bacteria, separate one by one, obtain Fu
Tea probiotic bacteria.FUCHA probiotic bacteria mycopowder is made into afterwards
Weigh 150 grams of FUCHA probiotic bacteria mycopowder, 150 grams of Bifidobacteria powder（Buy in shop）, 300 grams of Folium Ipomoea powder, Massa Medicata Fermentata
100 grams, 20 grams of magnesium citrate, 40 grams of taurine, 0. 1 grams of tryptophan, 100 grams of fruit oligose, 100 grams of galactooligosacchari(es, white carbon
200 grams, microorganism formulation is uniformly mixed to prepare, by being sprayed in fish meal outside 5% dosage, carries out feeding and throw.
Embodiment 3：
Weigh 100 grams of FUCHA probiotic bacteria mycopowder, 200 grams of Bifidobacteria powder, 200 grams of Folium Ipomoea powder, 50 grams of Massa Medicata Fermentata, citric acid
10 grams of magnesium, 10 grams of taurine, 0.3 gram of tryptophan, 50 grams of fruit oligose, 50 grams of galactooligosacchari(es, 100 grams of white carbon, uniform mixing system
Microorganism formulation, by uniform mixing outside 8% dosage and fish meal, carry out feeding and throw.
Contrast experiment 1：
The separation of strain:Precision weighs 1.00g tea samples, adds sterilized water to 500mL, soaks 5h.10 μ are taken with aseptic liquid-transfering gun
In L injection plating mediums, shake up, be placed in culture in 28.5 DEG C of constant incubators, bacterium colony covers with whole flat board, root after 3～5d
According to the morphological characteristic of just point bacterium colony, with a small amount of mycelium of each bacterium colony of Inoculating needle picking, preprepared culture medium is seeded in
It is interior, after bacterium colony grows, screened and purification according to each bacterium colony feature for growing, so repeatedly, until the growth conditions of bacterium colony
It is considered as the bacterium colony of single culture when showing consistent with morphological characteristic.Then with Eurotium Cristatum as dominant bacteria, separate one by one, obtain Fu
Tea probiotic bacteria.FUCHA probiotic bacteria mycopowder is made into afterwards.
Weigh 150 grams of FUCHA probiotic bacteria mycopowder, 150 grams of Bifidobacteria powder（Buy in shop）, 300 grams of Folium Ipomoea powder,
100 grams of Massa Medicata Fermentata, 20 grams of magnesium citrate, 40 grams of taurine, 0. 1 grams of tryptophan, 100 grams of fruit oligose, 100 grams of galactooligosacchari(es, Linesless charcoal
Black 200 grams, microorganism formulation is mixed to prepare, by 4%, 8%, 12% dosage Ctenopharyngodon idellus special feed is respectively added to（Tianjin Aquatic product grinds
Study carefully institute, special feed）In be configured to test feedstuff, and will not contain this compositions feed of grass carp as control feed.Feed just
Initial body weight（12.0±1.5）The Ctenopharyngodon idellus of g count Gain weight after 12 weeks, and the challenge test of Aeromonas hydrophila is carried out afterwards, unite
Meter survival rate, the results are shown in Table 1：
| Rate of body weight gain（%） | Survival rate（%） | |
| 4% addition group | 8.18 | 68.13 |
| 8% addition group | 14.73 | 80.28 |
| 12% addition group | 14.56 | 79.10 |
| Matched group | 7.35 | 65.48 |
As shown in Table 1, experimental group rate of body weight gain is 8.18～14.73%, and matched group rate of body weight gain is 7.35%；Infection Aeromonas hydrophila
Afterwards, experimental group survival rate is 68.13～80.28%, and matched group survival rate is 65.48%；Tissue slice is carried out to testing fish intestinal
After find, there is obscission in matched group intestinal villi, and experimental group is relatively complete.
Contrast experiment 2：
The separation of strain:Precision weighs 1.00g tea samples, adds sterilized water to 500mL, soaks 5h.10 μ are taken with aseptic liquid-transfering gun
In L injection plating mediums, shake up, be placed in culture in 28.5 DEG C of constant incubators, bacterium colony covers with whole flat board, root after 3～5d
According to the morphological characteristic of just point bacterium colony, with a small amount of mycelium of each bacterium colony of Inoculating needle picking, preprepared culture medium is seeded in
It is interior, after bacterium colony grows, screened and purification according to each bacterium colony feature for growing, so repeatedly, until the growth conditions of bacterium colony
It is considered as the bacterium colony of single culture when showing consistent with morphological characteristic.Then with Eurotium Cristatum as dominant bacteria, separate one by one, obtain Fu
Tea probiotic bacteria.FUCHA probiotic bacteria mycopowder is made into afterwards.
Weigh 150 grams of FUCHA probiotic bacteria mycopowder, 150 grams of Bifidobacteria powder（Buy in shop）, 300 grams of Folium Ipomoea powder,
100 grams of Massa Medicata Fermentata, 20 grams of magnesium citrate, 40 grams of taurine, 0. 1 grams of tryptophan, 100 grams of fruit oligose, 100 grams of galactooligosacchari(es, Linesless charcoal
Black 200 grams, microorganism formulation is mixed to prepare, Carassius auratuss special feed is sprayed on respectively by 4%, 6%, 8% dosage using trypsin method technology
（Liyang Zhengchang Feed Science ＆ Technology Co., Ltd., Carassius auratuss special feed）Surface will not contain the crucian carp of this compositions as experiment feedstuff
Fish meal is used as control feed.Feed original body mass（10.0±1.2）The Carassius auratuss of g count Gain weight after 12 weeks, carry out afterwards
The challenge test of Aeromonas hydrophila, counts survival rate, the results are shown in Table 2：
| Rate of body weight gain（%） | Survival rate（%） | |
| 4% addition group | 21.37 | 78.96 |
| 6% addition group | 24.33 | 89.67 |
| 8% addition group | 24.64 | 88.17 |
| Matched group | 19.76 | 70.06 |
As shown in Table 2, experimental group rate of body weight gain is 21.37～24.64%, and matched group rate of body weight gain is 19.76%；Infect thermophilic aqueous vapor unit cell
After bacterium, experimental group survival rate is 78.96～89.67%, and matched group survival rate is 70.06%；Tissue is carried out to experiment fish intestinal to cut
Find after piece, matched group intestinal villi has obscission, and experimental group is relatively complete.Jing inventor test result indicate that, by 5
～10% dosage is added to above-mentioned composition in the feedstuff of freshwater fish, tests fish rate of body weight gain and infection Aeromonas hydrophila
Survival rate afterwards is significantly improved.Present invention operation is upper less demanding, is easily mastered and implements, will not to cultured fishes and it is artificial into
Injury.
Certainly, described above not limitation of the present invention, the present invention is also not limited to the example above.The art
In the essential scope of the present invention, change, remodeling, addition or the replacement made should also belong to the present invention's to those of ordinary skill
Protection domain.
Claims (6)
1. fresh-water fishes microorganism formulation compositionss and application thereof, it is characterised in that：The microorganism formulation compositionss are by following original
Material and its parts by weight composition：10～20 parts of FUCHA probiotic bacteria mycopowder, 10～20 parts of Bifidobacteria powder, Folium Ipomoea powder 20～
40 parts, 5～10 parts of Massa Medicata Fermentata, 1～5 part of magnesium citrate, 1～5 part of taurine, 0.01～0.05 part of tryptophan, fruit oligose 5～15
Part, 5～15 parts of galactooligosacchari(es, 10～30 parts of white carbon.
2. fresh-water fishes microorganism formulation compositionss according to claim 1 and application thereof, it is characterised in that：The microorganism
Preparation compositions are made up of following raw material and its parts by weight：15 parts of FUCHA probiotic bacteria mycopowder, 15 parts of Bifidobacteria powder, Radix Ipomoeae
30 parts of leaf powder, 10 parts of Massa Medicata Fermentata, 2 parts of magnesium citrate, 4 parts of taurine, 0.01 part of tryptophan, 10 parts of fruit oligose, galactooligosacchari(es 10
Part, 20 parts of white carbon.
3. fresh-water fishes microorganism formulation compositionss and application thereof, it is characterised in that：The microorganism formulation compositionss are used to prepare
The purposes of the microorganism formulation compositionss of fresh-water fishes intestinal health can be improved.
4. fresh-water fishes microorganism formulation compositionss according to claim 3 and application thereof, it is characterised in that：The microorganism
Addition of the preparation compositions in fresh water fish feed is 5～10%.
5. fresh-water fishes microorganism formulation compositionss according to claim 4 and application thereof, it is characterised in that：The microorganism
Preparation compositions outside 5～8% dosage by being sprayed in cultured freshwater fish feedstuff.
6. fresh-water fishes microorganism formulation compositionss according to claim 4 and application thereof, it is characterised in that：The microorganism
Preparation compositions are mixed in cultured freshwater fish feedstuff by 8～10% dose uniformity.
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Application publication date: 20170419 |