CN106554982A - A kind of ultrasonically auxiliary extracting method of LBP-X - Google Patents
A kind of ultrasonically auxiliary extracting method of LBP-X Download PDFInfo
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- CN106554982A CN106554982A CN201510634517.0A CN201510634517A CN106554982A CN 106554982 A CN106554982 A CN 106554982A CN 201510634517 A CN201510634517 A CN 201510634517A CN 106554982 A CN106554982 A CN 106554982A
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- lbp
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- matrimony vine
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Abstract
The invention discloses a kind of extraction process of LBP-X, including step：(1) matrimony vine is placed at 50 DEG C and is vacuum dried 24h, after cooling, crushed with pulverizer, cross 100 mesh sieves；(2) matrimony vine powder adds petroleum ether extraction, and defatted seed flour is dried under vacuum to constant weight at 60 DEG C；(3) defatted seed flour adds cellulase solution, and ultrasound a period of time, is subsequently placed in boiling water bath and heats the enzyme that goes out, suction filtration after cooling, solution concentration；(4) concentrate adds the absolute ethyl alcohol of 4 times of volumes, and 12h alcohol precipitations are stood at 4 DEG C.15min is centrifuged under the conditions of 4200rpm, precipitation is taken, with absolute ethanol washing 3 times, is freezed, is obtained Thick many candies.The inventive method tool compared with conventional hot water's extraction has great advantage, and has broad application prospects in plant extraction field.
Description
Technical field
The invention belongs to technical field of polysaccharide extraction, and in particular to a kind of extracting method of LBP-X.
Background technology
Matrimony vine is many quil machakas, and drought-resistant ability is very strong, and its berry fruit of Chinese wolfberry is China's tradition rare traditional Chinese medicine.The fruit of Chinese wolfberry is more rich in plant
Sugar, protein, vitamins and other nutritious components, with liver protection moistening lung, improving kidney and eyes, Mi Gu granule, consumptive disease of dispelling, strengthening the bones and muscles, anti-aging, drop blood
The pharmacological actions such as sugar, reducing blood lipid and strengthen immunity, are constantly subjected to the great attention of China and foreign countries' medical science and dietotherapy expert.LBP-X is a kind of important
Biological regulator, its health care are the focuses of current function of health food factor research.There is to body LBP-X nospecific immunity to reconcile work(
Can, immunity of organisms is can adjust, suppresses tumour growth and cell mutation, anti-aging to improve adaptability, be a kind of important work(of health food
Energy property additive, has good application prospect.
Traditional LBP-X extracting method is petroleum ether degreasing paraffin removal, water-soluble heating extraction, ethanol precipitation, centrifugation, drying bu sublimation.
LBP-X is extracted in this way, and product purity is low, due to long-time heating, the easy brown stain of product, polysaccharide yield is low and unstable product quality,
Seldom adopt.
The content of the invention
It is an object of the invention to provide a kind of extraction efficiency is high, extraction time is short, the novel technology for extracting of mild condition.
For achieving the above object, the invention provides a kind of extraction process of LBP-X, comprises the following steps：
(1) matrimony vine is placed at 50 DEG C and is vacuum dried 24h, after cooling, crushed with pulverizer, cross 100 mesh sieves；(2) matrimony vine powder adds stone
Defatted seed flour is dried under vacuum to constant weight at 60 DEG C by oily ether extraction；(3) defatted seed flour adds cellulase solution, and ultrasound a period of time, then
It is placed in boiling water bath and heats the enzyme that goes out, suction filtration after cooling, solution concentration；(4) concentrate adds the absolute ethyl alcohol of 4 times of volumes, at 4 DEG C stands 12h
Alcohol precipitation.15min is centrifuged under the conditions of 4200rpm, precipitation is taken, with absolute ethanol washing 3 times, is freezed, is obtained Thick many candies.
Preferably, in step (2), extraction temperature is 90 DEG C.
Preferably, in step (3), extraction time is 10-50min, and ultrasonic power is 80W.
Preferably, in step (3), extraction temperature is 40-80 DEG C.
Preferably, the concentration of step (3) cellulase is 2%.
The extracting method of LBP-X of the present invention, compared with conventional hot water extracts, tool has great advantage, and takes efficiency high, and extraction time is short, protects
The structure and biologically active of polysaccharide are protected, has been had broad application prospects in plant extraction field.
Specific embodiment
Embodiment 1
(1) matrimony vine is placed at 50 DEG C and is vacuum dried 24h, after cooling, crushed with pulverizer, cross 100 mesh sieves；(2) matrimony vine powder adds stone
Oily ether is extracted at 90 DEG C, and defatted seed flour is dried under vacuum to constant weight at 60 DEG C；(3) defatted seed flour adds cellulase solution in ultrasonic power 80W
Under, ultrasonic 20min is subsequently placed in boiling water bath and heats the enzyme that goes out, suction filtration after cooling, solution concentration；(4) concentrate adds the anhydrous second of 4 times of volumes
Alcohol, stands 12h alcohol precipitations at 4 DEG C.15min is centrifuged under the conditions of 4200rpm, precipitation is taken, with absolute ethanol washing 3 times, is freezed, is obtained slightly
Polysaccharide.
Embodiment 2
(1) matrimony vine is placed at 50 DEG C and is vacuum dried 24h, after cooling, crushed with pulverizer, cross 100 mesh sieves；(2) matrimony vine powder adds stone
Oily ether is extracted at 90 DEG C, and defatted seed flour is dried under vacuum to constant weight at 60 DEG C；(3) defatted seed flour adds cellulase solution in ultrasonic power 80W
Under, ultrasonic 30min is subsequently placed in boiling water bath and heats the enzyme that goes out, suction filtration after cooling, solution concentration；(4) concentrate adds the anhydrous second of 4 times of volumes
Alcohol, stands 12h alcohol precipitations at 4 DEG C.15min is centrifuged under the conditions of 4200rpm, precipitation is taken, with absolute ethanol washing 3 times, is freezed, is obtained slightly
Polysaccharide.
Above example is only illustrating technical scheme, rather than is limited, although carrying out to the present invention with reference to above-described embodiment
Detailed description, for the person of ordinary skill of the art, still can modify to the technical scheme described in above-described embodiment, or
Equivalent is carried out to which part technical characteristic, and these are changed or replace, do not make the essence of appropriate technical solution depart from of the presently claimed invention
The spirit and scope of technical scheme.
Claims (6)
1. a kind of extraction process of LBP-X, it is characterised in that comprise the following steps：(1) matrimony vine is placed at 50 DEG C and is vacuum dried 24h, it is cold
But after, crushed with pulverizer, cross 100 mesh sieves；(2) matrimony vine powder adds petroleum ether extraction, and defatted seed flour is dried under vacuum to constant weight at 60 DEG C；
(3) defatted seed flour adds cellulase solution, and ultrasound a period of time, is subsequently placed in boiling water bath and heats the enzyme that goes out, suction filtration after cooling, solution concentration；
(4) concentrate adds the absolute ethyl alcohol of 4 times of volumes, and 12h alcohol precipitations are stood at 4 DEG C.15min is centrifuged under the conditions of 4200rpm, precipitation is taken,
With absolute ethanol washing 3 times, freeze, obtain Thick many candies.
2. the extraction process of LBP-X according to claim 1, it is characterised in that extraction temperature is 90 DEG C in step (2).
3. the extraction process of LBP-X according to claim 1, it is characterised in that ultrasonic time is 10-50min in step (3), is surpassed
Acoustical power is 80W.
4. the extraction process of LBP-X according to claim 1, it is characterised in that extraction temperature is 40-80 DEG C in step (3).
5. the extraction process of LBP-X according to claim 1, it is characterised in that the concentration of step (3) cellulase is 2%.
6. according to claim 1-5 any one extract matrimony vine in polysaccharide method obtained in LBP-X.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510634517.0A CN106554982A (en) | 2015-09-29 | 2015-09-29 | A kind of ultrasonically auxiliary extracting method of LBP-X |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510634517.0A CN106554982A (en) | 2015-09-29 | 2015-09-29 | A kind of ultrasonically auxiliary extracting method of LBP-X |
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| CN106554982A true CN106554982A (en) | 2017-04-05 |
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| CN201510634517.0A Pending CN106554982A (en) | 2015-09-29 | 2015-09-29 | A kind of ultrasonically auxiliary extracting method of LBP-X |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109503727A (en) * | 2018-11-06 | 2019-03-22 | 宁夏北方天成生物科技有限公司 | A kind of method of purification of polysaccharides |
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2015
- 2015-09-29 CN CN201510634517.0A patent/CN106554982A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109503727A (en) * | 2018-11-06 | 2019-03-22 | 宁夏北方天成生物科技有限公司 | A kind of method of purification of polysaccharides |
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Application publication date: 20170405 |