CN106544241A - A kind of cordyceps wine preparation method attractive in appearance - Google Patents
A kind of cordyceps wine preparation method attractive in appearance Download PDFInfo
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- CN106544241A CN106544241A CN201510601382.8A CN201510601382A CN106544241A CN 106544241 A CN106544241 A CN 106544241A CN 201510601382 A CN201510601382 A CN 201510601382A CN 106544241 A CN106544241 A CN 106544241A
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Abstract
The present invention relates to a kind of preparation method of cordyceps militaris liquor attractive in appearance, the method comprises the steps：Step 1, cordyceps species are pressed in media surface with Perforated decking during solid culture；Step 2, treats that coremium grows to suitable height from aperture, cover plate is separated with culture medium with the coremium of culture medium contact portion；Step 3, by cover plate and is grown on coremium therein and is placed in container together；Step 4, notes wine in container；With step 5, storage.Preparation method of the present invention both ensure that coremium growing way was homogeneous, can also remove culture medium in wine soaked long caused by wine research of chaotic phenomenon, it is ensured that the quality of cordyceps militaris liquor.
Description
Technical field
The present invention relates to a kind of Cordycepses method for preparing medicated wine, and in particular to a kind of with cover plate culture Cordycepses with holes, then the method for preparing cordyceps militaris liquor.
Background technology
Cordyceps militaris (L.) Link. (Cordyceps) is Ascomycotina, Ergota Zoopagales, Cordycepses section, the type sepecies of Cordyceps, scientific name be [Cordyceps militaris (Vuill.) Fr.].Modern science not only has special nutritive value in proving Cordyceps militaris (L.) Link., and has obvious medical value.Wherein especially based on cordycepic acid, cordycepin, adenosine, Cordyceps polysaccharide.With functions such as tonifying the lung the moon, kidney-replenishings, the Main Function of Cordyceps militaris (L.) Link. is to control kidney deficiency, impotence and seminal emission, soreness of waist and knee joint, eak after being ill.The traditional Chinese medical science thinks which plays strengthening the body resistance effect, has significant curative effect to senile chronic bronchitis, pulmonary heart disease, can improve liver detoxification ability, play liver protection effect, improves body antiviral and capability of resistance to radiation.The traditional Chinese medical science thinks that Cordycepses enter lung kidney two warp, can tonifying the lung it is cloudy, and can kidney-replenishing, cure mainly kidney deficiency, impotence and seminal emission, soreness of waist and knee joint, eak after being ill, chronic cough are weak, phthisical cough expectorant blood, spontaneous sweating etc., be it is unique it is a kind of can be while balancing, adjusting the Chinese medicine of negative and positive.
It is the usual alimentation method for adopting that Cordycepses are steep in wine, and its brew process is：By Cordycepses cultivate in bottle and pour into Chinese liquor, seal, to place appropriate natural law i.e. drinkable.Using this brewing method, Cordycepses cultivation cycle is very long, and growing way is uneven, just differs, and density differs, and morphological appearance is all poor.
The content of the invention
For problems of the prior art, the invention provides a kind of Cordycepses method for preparing medicated wine, during Cordycepses solid culture, in culture medium, one piece of narrow meshed cover plate of pressure, allows sporophore progressively to grow from the aperture of cover plate to the method.When certain altitude is grown to, the culture under cover plate is cut along cover plate, then cut the culture medium of cover plate bottom, be put in the container for containing wine, note wine, storage.So both ensure that Cordyceps sporophore growing way was homogeneous, can also remove culture medium in wine soaked long caused by wine research of chaotic phenomenon.Ensure that the quality of cordyceps militaris liquor.
The purpose of the present invention is achieved by the following technical solution：
Step 1, cordyceps species are pressed in media surface with Perforated decking during solid culture；
Step 2, treats that coremium grows to suitable height from aperture, cover plate is separated with culture medium with the coremium of culture medium contact portion；
Step 3, by cover plate and is grown on coremium therein and is placed in container together；
Step 4, notes wine in container；With
Step 5, storage.
Further, during solid culture described in step 1, Perforated decking is pressed in into media surface after light culture terminates；Further, terminate light culture when mycelium is covered with culture medium, switch to illumination cultivation；Further, the culture medium that Perforated decking selects its surface mycelial growth good is pushed.
Further, the material that cover plate described in step 1 is more than base liquor and is not reacted with base liquor by density is made, such as plasterboard, glass plate, corrosion resistant plate etc.；The cover plate shape depends on the shape of vessel port, and its area is slightly less than container open area；The small aperture of the cover plate is 4～6mm, and pitch of holes is 1～2mm；Further, the small aperture of the cover plate is 5～6mm.
Further, suitable height described in step 2 is 4～8cm；Further, select its riotous growth and the coremium of neat and consistent is separated；Further, depending on selected coremium height is with container height, it is 2～3cm away from vessel port distance, coremium difference in height is less than 2cm；Further, selected coremium difference in height is less than 1cm.
Further, step 4 note capacity for liquor is coremium：Base liquor (w/w)=10-20：80-90.
Further, step 4 note wine method is：Injection base liquor stands 30～60min to being higher by 1～2cm of coremium, notes wine again at 1～2cm of bottleneck.
Further, step 5 storage requirement is：Relative humidity 50～70%, 10～30 DEG C of temperature.
Further, step 5 storage natural law is more than 45 days.
The incubation of Cordycepses of the present invention is：Cordyceps species are expanded by liquid culture；Strain after amplification carries out solid culture.The liquid culture and solid culture method can refer to Cordycepses cultural method disclosed in prior art.Liquid culture is, for the purpose of strain amplification, can to adopt conventional strain propagation method, including the combination of any one or a few method in slant culture, shake-flask culture, seed tank culture, fermentor cultivation；Used medium is the conventional fluid medium in this area, such as PSA culture medium or yeast extract powder-aminoacids complex-sucrose medium, yeast extract powder-white sugar-soy bean protein hydrolysate culture medium, wheat bran liquor-white sand sugar culture-medium etc.；More preferably PSA culture medium or yeast extract powder-aminoacids complex-sucrose medium；Further, each component ratio of PSA culture medium is：Rhizoma Solani tuber osi 15-25%, sucrose 1-5%, agar 1-5%；Yeast extract powder-each component ratio of aminoacids complex-sucrose medium is：Yeast extract powder 0.5-2%, aminoacids complex 0.2-1%, sucrose 2-5%.Further, each component ratio of PSA culture medium is：Rhizoma Solani tuber osi 20%, sucrose 2%, agar 2%；Yeast extract powder-each component ratio of aminoacids complex-sucrose medium is：Yeast extract powder 1%, aminoacids complex 0.5%, sucrose 3.5%.
Solid medium is light culture in the mycelial growth stage, and temperature is 18～23 DEG C, relative humidity 65～85%, being covered with mycelium to culture medium, incubation time generally 6～8 days；Coremium growth stage switchs to illumination cultivation, and cultivation temperature is 18～20 DEG C, relative humidity 80～85%, in coremium late stage of culture, the circulation and exchange of room air are gradually increased, cultivation temperature brings up to 23～25 DEG C, to coremium length to 4-8cm, incubation time generally 35～50 days.Solid medium is the culture medium with corn, agricultural crop straw, crops cot, commodity trees branch or plant stem as main component；Wherein preferred grain culture medium；The corn may be selected from any one or a few in Semen Tritici aestivi, Semen Maydiss, rice, Semen setariae, analysis for soybean powder, Semen Fagopyri Esculenti, Fructus Hordei Vulgaris, Herba bromi japonici, brown rice, Semen oryzae sativae.Further, adjuvant, such as white sugar, yeast extract powder, vitamin B1, soybean protein, aminoacids complex etc. are also included in solid medium.
In the present invention, Cordyceps militaris (L.) Link. [Cordyceps militaris (Vuill.) Fr.] used is dispersed species.Cordyceps militaris (L.) Link. is distributed in the provinces and regions such as Hubei, Hebei, Jilin, Shaanxi, Yunnan, Guangxi, Guizhou, Sichuan, Taiwan.Acquisition method according to general Chinese crude drug can all be adopted, and be strain known in the state of the art, it is possible to which (such as edible fungi factory, institute), purchase is obtained from commercial channels.
The beneficial effects of the present invention is：
1. good looking appearance：The present invention fixes Cordyceps militaris (L.) Link. using cover plate with holes so as in vertical state, can guarantee that the character stable and consistent of cordyceps militaris liquor, elegant in appearance.2. quality is high：Due to eliminating solid medium, in cordyceps militaris liquor storage process, the material not having in culture is separated out, it is ensured that wine and women-sensual pursuits is exquisitely carved, apparent, and commercial value is higher.3. active constituent content is high：Cordyceps militaris liquor its adenosine content prepared by the inventive method reaches 20 μ g/ml, HEA (i.e. N6- (2- ethoxys) adenosines, also known as cocoon lavendustin, it is the endemic element of Cordycepses, as one of the quality control index of cordyceps product) content reaches 42 μ g/ml, is significantly higher than the method that commonly steep in wine.
Description of the drawings
Cordyceps militaris liquor prepared by Fig. 1 present invention
Fig. 2 Perforated deckings
Effect experimental examples
1 cover plate method of the present invention of experimental example is compared with conventional method effect of steeping in wine
First, experimental technique
Cordyceps militaris liquor is prepared by the following two kinds method, every kind of method arranges 6 groups of repetitions：
Method one, conventional brewing method：The cordyceps species that 1 amplification culture of Example is obtained, direct inoculation carry out solid culture by embodiment 4 in wine holding container, after culture terminates, note wine as described in Example 10 and store；
Method two, cover plate method of the present invention：The cordyceps species that 1 amplification culture of Example is obtained carry out solid culture by embodiment 4, after light culture terminates carry out pressing plate and cutting by embodiment 9, and cover plate and coremium are placed in container together, note wine as described in Example 10 and store.
Investigate cordyceps militaris liquor outward appearance and active constituent content prepared by distinct methods.
2nd, experimental result
The results are shown in Table 1.
Cordyceps militaris liquor effect prepared by 1 distinct methods of table compares (n=6)
2 different cover plate method effects of experimental example compare
First, experimental technique
Cordyceps militaris liquor is prepared by the following two kinds method, every kind of method arranges 6 groups of repetitions：
Method one：The cordyceps species that 1 amplification culture of Example is obtained carry out solid culture by embodiment 4, after culture terminates, the coremium for taking riotous growth and neat and consistent is harvested, and is inserted in the aperture of cover plate, cover plate and coremium are placed in container, wine are noted as described in Example 10 and is stored；
Method two：The cordyceps species that 1 amplification culture of Example is obtained carry out solid culture by embodiment 4, after light culture terminates carry out pressing plate and cutting by embodiment 9, and cover plate and coremium are placed in container together, note wine as described in Example 10 and store.
Investigate the difference that distinct methods prepare effect.
2nd, experimental result
The results are shown in Table 2.
Cordyceps militaris liquor effect prepared by 2 distinct methods of table compares (n=6)
Specific embodiment
The amplification culture of 1 cordyceps species of embodiment
Slant culture：The cordyceps species for isolating and purifying are inoculated in slant tube, then the slant tube of inoculation strain is put into into 22 DEG C of incubators, incubation time is 7 days, treats that mycelia covers with test tube；
Shake-flask culture：Load 200m1 fluid mediums in 500m1 triangular flasks, autoclaving 30 minutes under 0.11Mpa pressure, it is cooled to room temperature, the strain of 1 slant tube is inoculated in 500m1 triangular flask culture medium, it is placed in constant-temperature shaking incubator, 22 ± 1 DEG C of temperature, cultivates under the conditions of 150 revs/min, and incubation time is 3 days；
Seed tank culture：Load 20L fluid mediums in 50L airlift fermentors, culture medium temperature is more than 95 DEG C, add edible defoaming agent, addition is the 0.03% of culture medium weight, under 0.11Mpa pressure, is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, when being cooled to 20 DEG C, 4 bottles of above-mentioned cultured 500m1 shaking flasks strains are accessed into culture medium, cultivation temperature is 22 ± 1 DEG C, and ventilation is 1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, after reaching exponential phase, whole volume of culture is 20L.
Fermentor cultivation：Load 200L fluid mediums in 500L airlift fermentors, culture medium temperature is more than 95 DEG C, add edible defoaming agent, addition is the 0.03% of culture medium weight, under 0.11Mpa pressure, is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, when being cooled to 20 DEG C, above-mentioned cultured 200L seed tanks seed is all accessed into culture, cultivation temperature is 22 ± 1 DEG C, and ventilation is 1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, after reaching exponential phase.Whole volume of culture is 200L.
Slant tube culture medium：Rhizoma Solani tuber osi liquor 20%, white sugar 3.5%, yeast extract 0.5%；
Fluid medium in shaking flask, seed tank and fermentation tank：Containing yeast extract powder 0.5%, aminoacids complex 1%, white sugar 3.5%, surplus moisturizing to 100%, pH value are 6.5.
The amplification culture of 2 cordyceps species of embodiment
Slant culture：The Cordyceps militaris spawn for isolating and purifying is inoculated in slant tube, then the slant tube of inoculation strain is put into into 22 DEG C of incubators, incubation time is 7 days, treats that mycelia covers with test tube；
Shake-flask culture：Load 200m1 fluid mediums in 500m1 triangular flasks, autoclaving 30 minutes under 0.11Mpa pressure, it is cooled to room temperature, the strain of 1 slant tube is inoculated in 500m1 triangular flask culture medium, it is placed in constant-temperature shaking incubator, 22 ± 1 DEG C of temperature, cultivates under the conditions of 150 revs/min, and incubation time is 3 days；
Seed tank culture：Load 20L fluid mediums in 50L airlift fermentors, culture medium temperature is more than 95 DEG C, add edible defoaming agent, addition is the 0.03% of culture medium weight, under 0.11Mpa pressure, is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, when being cooled to 20 DEG C, 4 bottles of above-mentioned cultured 500m1 shaking flasks strains are accessed into culture medium, cultivation temperature is 22 ± 1 DEG C, and ventilation is 1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, after reaching exponential phase, whole volume of culture is 20L.
Slant tube culture medium：Contain 200 grams of Rhizoma Solani tuber osi liquors per 1 liter of liquid, 20 grams of sucrose, 20 grams of agar, to 1000 milliliters, pH value is 6.5 for remaining moisturizing；
Shaking flask and seed tank culture base：Contain 30 grams of yeast extract powders per 1 liter of liquid, 30 grams of white sugars, 5 grams of soy bean protein hydrolysates add water and mend to 1000 milliliters, and pH value is 6.5.
The amplification culture of 3 cordyceps species of embodiment
Slant culture：The cordyceps species for isolating and purifying are inoculated in slant tube, then the slant tube of inoculation strain is put into into 22 DEG C of incubators, incubation time is 7 days, treats that mycelia covers with test tube；
Seed tank culture：Load 20L fluid mediums in 50L gas-lifting type seed tanks, culture medium temperature is more than 95 DEG C, add edible defoaming agent, addition is the 0.03% of culture medium weight, under 0.11Mpa pressure, is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, when being cooled to 20 DEG C, the strain of 4 bottles of above-mentioned slant tubes is accessed into culture medium, cultivation temperature is 22 ± 1 DEG C, and ventilation is 1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, after reaching exponential phase, whole volume of culture is 20L.
Fermentor cultivation：Load 200L fluid mediums in 500L airlift fermentors, culture medium temperature is more than 95 DEG C, add edible defoaming agent, addition is the 0.03% of culture medium weight, under 0.11Mpa pressure, is passed through steam and is heated to 121 DEG C, and pressurize 30-45 minutes；After sterilizing, when being cooled to 20 DEG C, above-mentioned cultured 200L seed tanks seed is all accessed into culture, cultivation temperature is 22 ± 1 DEG C, and ventilation is 1：0.5V/V min, pressurize 4.903-7.0845 × 1043 days aerobic culture of Pa, Jing, after reaching exponential phase.Whole volume of culture is 200L.
Slant tube culture medium：Contain 200 grams of Rhizoma Solani tuber osi liquors per 1 liter of liquid, 20 grams of sucrose, 20 grams of agar, to 1000 milliliters, pH value is 6.5 for remaining moisturizing；
Seed tank and fermentation tank culture medium：Contain 40 grams of wheat bran liquors per 1 liter of liquid, 30 grams of white sugars, to 1000 milliliters, pH value is 6.5 for remaining moisturizing.
4 solid culture of embodiment
Solid medium：Nutritional solution is made in the ratio of white sugar 3.5%, yeast extract powder 0.5%, VB11 0mg/1000ml, rice and nutritional solution are pressed into 1:1.6 proportions are formed.The culture vessel for installing culture medium is placed in autoclave, sterilize at 121 DEG C 30-50min；After sterilizing, culture vessel dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
Inoculation：Before inoculation culture vessel first in superclean bench or hundred grades of laminar flow hoods (under) use ultraviolet light 0.5h；Each culture vessel is inoculated into the strain that 1～3 Jing amplification culture of embodiment is obtained on solid medium by 7% inoculum concentration, and postvaccinal container is put into culturing room's culture.
Condition of culture：The mycelia culture stage：Send out bacterium room temperature and be preferably 18～23 DEG C, relative air humidity 65%～85%, shading；6～8d of indoor cultivation, covers with mycelia；Coremium growth stage：Treat that mycelia is covered with whole box face and prick deeply cassette bottom, start to see light, but avoid sun light direct beam, adjustment cultivation temperature is 18～20 DEG C, humidity 80%～85%；In coremium late stage of culture, the circulation and exchange of room air are gradually increased, cultivation temperature brings up to 23～25 DEG C.If cultivation stage finds that pollution should be removed immediately.Coremium length is treated to 4～8cm, culture terminates.
5 solid culture of embodiment
Solid medium：Nutritional solution is made in the ratio of white sugar 3.5%, yeast extract powder 0.5%, VB11 0mg/1000ml, Semen setariae and nutritional solution are pressed into 1:1.6 proportions are formed.The culture vessel for installing culture medium is placed in autoclave, sterilize at 121 DEG C 30-50min；After sterilizing, culture vessel dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
Condition of culture is with embodiment 4.
6 solid culture of embodiment
Solid medium：After by brown rice cleaning control water, appropriate water is added, its weight ratio is brown rice：Water is 1：1.5, mix homogeneously；The culture vessel for installing culture medium is placed in autoclave, sterilize at 121 DEG C 30-50min；After sterilizing, culture vessel dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
Condition of culture is with embodiment 4.
7 solid culture of embodiment
Solid medium：After by Semen Tritici aestivi cleaning control water, appropriate water is added, its weight ratio is Semen Tritici aestivi:Water is 1：1.3, mix homogeneously；The culture vessel for installing culture medium is placed in autoclave, sterilize at 121 DEG C 30-50min；After sterilizing, culture vessel dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
Condition of culture is with embodiment 4.
8 solid culture of embodiment
Solid medium：Corn cob 69%, cotton seed hullss 16%, wood flour 11%, Gypsum Fibrosum 3%, Calx 1%, solid material：Water=1：1.5 (depending on feed moisture content) mixing is mixed thoroughly；The culture vessel for installing culture medium is placed in autoclave, sterilize at 121 DEG C 30-50min；After sterilizing, culture vessel dislocation buffering is indoor, naturally cools to less than 24 DEG C dislocation inoculations indoor.
Condition of culture is with embodiment 4.
9 pressing plate of embodiment and cutting
The cordyceps species of 1～3 amplification culture of embodiment are carried out into solid culture by 4～8 either method of embodiment, after light culture terminates, (diameter is slightly less than wine holding container mouth to take graphite plate, aperture 5mm, pitch of holes is 1mm, see Fig. 2), select that its surface mycelia is luxuriant, well-grown solid medium is pushed, coremium is grown from aperture.After solid culture terminates, select its surface coremium neat and consistent, the cover plate of height about 4～5cm or so, culture medium is cut along edge, and the contact surface along cover plate and culture medium removes the culture medium of cover plate bottom, by cover plate and be grown on coremium therein and be placed in container together.
10 note wine of embodiment and storage
Cleaning the desktop sterilized are selected, using the method for sterile working, base liquor is flowed down slowly along bottle inwall, injection base liquor stands 30～60min to being higher by 1～2cm of coremium, base liquor is re-injected at 1～2cm of bottleneck.
Select more to be dried, where cleaning, light and ventilation preferably, relative ambient humidity 70% or so, 25 DEG C or so of temperature；Container closure is tight, prevents wine leaking and " race degree "；Avoid intense direct illumination.Preserving in being put into wine cellar becomes orange to orange-yellow to wine and women-sensual pursuits in about 45 days or so, you can go out cellar for storing things for finished product (see Fig. 1).
Claims (10)
1. a kind of preparation method of cordyceps militaris liquor attractive in appearance, it is characterised in that the method comprises the steps：
Step 1, cordyceps species are pressed in media surface with Perforated decking during solid culture；
Step 2, treats that coremium grows to suitable height from aperture, by cover plate and culture medium contact site
The coremium for dividing is separated with culture medium；
Step 3, by cover plate and is grown on coremium therein and is placed in container together；
Step 4, notes wine in container；With
Step 5, storage.
2. preparation method as claimed in claim 1, it is characterised in that solid culture described in step 1
During, Perforated decking is pressed in into media surface after light culture terminates.
3. preparation method as claimed in claim 1, it is characterised in that step 1 Perforated decking is selected
The good culture medium of its surface mycelial growth is pushed.
4. preparation method as claimed in claim 1, it is characterised in that Perforated decking described in step 1
The material for being more than base liquor and do not reacted with base liquor by density is made, and the small aperture is
4～6mm, pitch of holes are 1～2mm.
5. preparation method as claimed in claim 4, it is characterised in that the cover plate be plasterboard,
Any one in glass plate or corrosion resistant plate.
6. preparation method as claimed in claim 1, it is characterised in that suitable height described in step 2
For 4～8cm.
7. preparation method as claimed in claim 1, it is characterised in that step 2 selects its growth cyclopentadienyl
Contain and the coremium of neat and consistent is separated, coremium difference in height is less than 2cm.
8. preparation method as claimed in claim 1, it is characterised in that step 4 note capacity for liquor is falx
Beam：Base liquor (w/w)=10-20：80-90.
9. preparation method as claimed in claim 1, it is characterised in that step 4 note wine method is：
Injection base liquor stands 30～60min to being higher by 1～2cm of coremium, notes wine again to apart from bottleneck
At 1～2cm.
10. preparation method as claimed in claim 1, it is characterised in that step 5 storage requirement is：
Relative humidity 50～70%, 10～30 DEG C of temperature；Storage natural law is more than 45 days.
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| CN101103678A (en) * | 2007-06-20 | 2008-01-16 | 彭泽福 | Edible mushroom fungi bed cultivation bed surface directional fruiting cover |
| CN102242044A (en) * | 2010-05-14 | 2011-11-16 | 浙江泛亚生物医药股份有限公司 | Cordyceps cicadae wine and preparation method thereof |
| CN104419602A (en) * | 2013-08-20 | 2015-03-18 | 浙江泛亚生物医药股份有限公司 | Cordyceps sinensis liquor, preparation and application thereof |
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2015
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103678A (en) * | 2007-06-20 | 2008-01-16 | 彭泽福 | Edible mushroom fungi bed cultivation bed surface directional fruiting cover |
| CN102242044A (en) * | 2010-05-14 | 2011-11-16 | 浙江泛亚生物医药股份有限公司 | Cordyceps cicadae wine and preparation method thereof |
| CN104419602A (en) * | 2013-08-20 | 2015-03-18 | 浙江泛亚生物医药股份有限公司 | Cordyceps sinensis liquor, preparation and application thereof |
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Effective date of registration: 20191224 Address after: 314200 Building 2, No. 1938, xinqun Road, Pinghu Economic Development Zone, Jiaxing City, Zhejiang Province Applicant after: Zhejiang Panya Health Food Co., Ltd. Address before: 314200 No. 1938, group road, Pinghu Economic Development Zone, Zhejiang, Jiaxing Applicant before: Zhejiang Faya Biological Pharmaceutical Co., Ltd. |
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