CN106537142B - 使用杂交的靶核酸检测 - Google Patents

使用杂交的靶核酸检测 Download PDF

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Publication number
CN106537142B
CN106537142B CN201580040576.3A CN201580040576A CN106537142B CN 106537142 B CN106537142 B CN 106537142B CN 201580040576 A CN201580040576 A CN 201580040576A CN 106537142 B CN106537142 B CN 106537142B
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China
Prior art keywords
region
oligonucleotides
target genomic
capture
fixed sequence
Prior art date
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CN201580040576.3A
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English (en)
Chinese (zh)
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CN106537142A (zh
Inventor
阿诺德·奥利芬特
雅各布·赞恩
卡拉·朱诺
帕特里克·伯加德
斯蒂芬妮·黄
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F Hoffmann La Roche AG
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A Ruiaosa Diagnostic Cos
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Publication date
Priority claimed from US14/450,144 external-priority patent/US20140342940A1/en
Application filed by A Ruiaosa Diagnostic Cos filed Critical A Ruiaosa Diagnostic Cos
Publication of CN106537142A publication Critical patent/CN106537142A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/519—Detection characterised by immobilisation to a surface characterised by the capture moiety being a single stranded oligonucleotide

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580040576.3A 2014-08-01 2015-07-29 使用杂交的靶核酸检测 Active CN106537142B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US14/450,144 2014-08-01
US14/450,144 US20140342940A1 (en) 2011-01-25 2014-08-01 Detection of Target Nucleic Acids using Hybridization
US14/453,396 US9567639B2 (en) 2010-08-06 2014-08-06 Detection of target nucleic acids using hybridization
US14/453,396 2014-08-06
PCT/US2015/042604 WO2016018986A1 (en) 2014-08-01 2015-07-29 Detection of target nucleic acids using hybridization

Publications (2)

Publication Number Publication Date
CN106537142A CN106537142A (zh) 2017-03-22
CN106537142B true CN106537142B (zh) 2019-11-19

Family

ID=55218263

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580040576.3A Active CN106537142B (zh) 2014-08-01 2015-07-29 使用杂交的靶核酸检测

Country Status (10)

Country Link
EP (1) EP3175236B1 (enExample)
JP (1) JP6793112B2 (enExample)
KR (1) KR20170036727A (enExample)
CN (1) CN106537142B (enExample)
AU (1) AU2015296602B2 (enExample)
CA (1) CA2955740A1 (enExample)
ES (1) ES2752237T3 (enExample)
MX (1) MX376755B (enExample)
SG (1) SG11201700765WA (enExample)
WO (1) WO2016018986A1 (enExample)

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US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
CA2921628A1 (en) 2013-08-19 2015-02-26 Singular Bio, Inc. Assays for single molecule detection and use thereof
WO2016134191A1 (en) 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof
EP3601599A4 (en) * 2017-03-23 2020-12-23 Apton Biosystems, Inc. POLYMORPHISM DETECTION WITH INCREASED ACCURACY
WO2018223057A1 (en) * 2017-06-02 2018-12-06 Affymetrix, Inc. Array-based methods for analysing mixed samples using different allele-specific labels, in particular for detection of fetal aneuploidies
WO2018223053A1 (en) * 2017-06-02 2018-12-06 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
EP3631008B1 (en) * 2017-06-02 2025-12-03 Affymetrix, Inc. Array-based methods for analysing mixed samples using differently labelled allele-specific probes
DE112019005108T5 (de) * 2018-10-12 2021-07-15 Nantomics, Llc Pränatale Reinheitsbeurteilungen mit Bambam

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000056927A3 (en) * 1999-03-19 2002-03-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
CN101506379A (zh) * 2006-05-22 2009-08-12 临床基因组学有限公司 检测方法
CN101985619A (zh) * 2003-10-08 2011-03-16 波士顿大学信托人 染色体异常的产前诊断方法
WO2013009175A1 (en) * 2011-07-08 2013-01-17 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
WO2012019193A3 (en) * 2010-08-06 2013-02-28 Ariosa Diagnostics, Inc. Assay systems for genetic analysis

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020042681A1 (en) * 2000-10-03 2002-04-11 International Business Machines Corporation Characterization of phenotypes by gene expression patterns and classification of samples based thereon
US20050100939A1 (en) * 2003-09-18 2005-05-12 Eugeni Namsaraev System and methods for enhancing signal-to-noise ratios of microarray-based measurements
US8620593B2 (en) * 2009-11-06 2013-12-31 The Chinese University Of Hong Kong Size-based genomic analysis
WO2011066476A1 (en) * 2009-11-25 2011-06-03 Quantalife, Inc. Methods and compositions for detecting genetic material
US10131947B2 (en) * 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
CA2824387C (en) * 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10196681B2 (en) * 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP2015508655A (ja) * 2012-02-14 2015-03-23 コーネル ユニバーシティー 組み合わせたヌクレアーゼ反応、連結反応、およびポリメラーゼ反応を用いて核酸配列、発現、またはコピー変化を相対的に定量するための方法
WO2013192292A1 (en) * 2012-06-21 2013-12-27 Justin Lamb Massively-parallel multiplex locus-specific nucleic acid sequence analysis
US9206417B2 (en) * 2012-07-19 2015-12-08 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000056927A3 (en) * 1999-03-19 2002-03-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
CN101985619A (zh) * 2003-10-08 2011-03-16 波士顿大学信托人 染色体异常的产前诊断方法
CN101506379A (zh) * 2006-05-22 2009-08-12 临床基因组学有限公司 检测方法
WO2012019193A3 (en) * 2010-08-06 2013-02-28 Ariosa Diagnostics, Inc. Assay systems for genetic analysis
WO2013009175A1 (en) * 2011-07-08 2013-01-17 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays

Also Published As

Publication number Publication date
EP3175236A1 (en) 2017-06-07
EP3175236B1 (en) 2019-08-21
EP3175236A4 (en) 2017-12-27
SG11201700765WA (en) 2017-02-27
CN106537142A (zh) 2017-03-22
AU2015296602A1 (en) 2017-02-16
WO2016018986A1 (en) 2016-02-04
MX2017001405A (es) 2017-05-17
JP6793112B2 (ja) 2020-12-02
CA2955740A1 (en) 2016-02-04
MX376755B (es) 2025-03-07
ES2752237T3 (es) 2020-04-03
AU2015296602B2 (en) 2021-09-16
JP2017526347A (ja) 2017-09-14
KR20170036727A (ko) 2017-04-03

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Patentee after: F. HOFFMANN-LA ROCHE AG

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Patentee before: ARIOSA DIAGNOSTICS, Inc.

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