CN106536728A - 用于核苷酸类似物的改进的掺入的修饰的聚合酶 - Google Patents

用于核苷酸类似物的改进的掺入的修饰的聚合酶 Download PDF

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Publication number
CN106536728A
CN106536728A CN201580033818.6A CN201580033818A CN106536728A CN 106536728 A CN106536728 A CN 106536728A CN 201580033818 A CN201580033818 A CN 201580033818A CN 106536728 A CN106536728 A CN 106536728A
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China
Prior art keywords
polymerase
transformation
polymerases
functionally equivalent
nucleotide
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CN201580033818.6A
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Chinese (zh)
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陈呈尧
艾琳·博马蒂
莫利·何
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Illumina Inc
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Illumina Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201580033818.6A 2014-06-27 2015-06-25 用于核苷酸类似物的改进的掺入的修饰的聚合酶 Pending CN106536728A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462018470P 2014-06-27 2014-06-27
US62/018,470 2014-06-27
PCT/US2015/037785 WO2015200693A1 (en) 2014-06-27 2015-06-25 Modified polymerases for improved incorporation of nucleotide analogues

Publications (1)

Publication Number Publication Date
CN106536728A true CN106536728A (zh) 2017-03-22

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CN201580033818.6A Pending CN106536728A (zh) 2014-06-27 2015-06-25 用于核苷酸类似物的改进的掺入的修饰的聚合酶

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US (3) US9765309B2 (enExample)
EP (2) EP3702471B1 (enExample)
JP (3) JP6737710B2 (enExample)
CN (1) CN106536728A (enExample)
AU (2) AU2015279794B2 (enExample)
DK (1) DK3161154T3 (enExample)
ES (2) ES2788949T3 (enExample)
PL (1) PL3161154T3 (enExample)
PT (1) PT3161154T (enExample)
WO (1) WO2015200693A1 (enExample)

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WO2020047695A1 (zh) * 2018-09-03 2020-03-12 深圳华大生命科学研究院 重组型kod聚合酶
CN112639090A (zh) * 2018-12-05 2021-04-09 亿明达股份有限公司 聚合酶、组合物及使用方法
WO2022082482A1 (zh) * 2020-10-21 2022-04-28 深圳华大生命科学研究院 重组型kod聚合酶
CN114846149A (zh) * 2019-12-23 2022-08-02 陈呈尧 用于不依赖于模板的核酸合成的方法和试剂盒
CN116286720A (zh) * 2023-03-29 2023-06-23 赛纳生物科技(北京)有限公司 一种dna聚合酶
WO2025260380A1 (zh) * 2024-06-21 2025-12-26 华大工程生物学长荡湖研究所 重组型kod聚合酶

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AU2018259206B2 (en) 2017-04-23 2024-07-11 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
CN110770353B (zh) 2017-04-23 2024-11-26 伊鲁米那股份有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
US20230011240A1 (en) 2018-09-11 2023-01-12 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
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US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
WO2021058438A1 (en) 2019-09-23 2021-04-01 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
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US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
TWI869710B (zh) 2021-09-29 2025-01-11 呈堯 陳 用於無模板ｒｎａ合成的核酸聚合酶變體、試劑套組及方法
WO2024039652A1 (en) 2022-08-16 2024-02-22 Aldevron, L.L.C. Cell-free method of producing synthetic circular nucleic acid
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
WO2024155578A1 (en) * 2023-01-16 2024-07-25 The Regents Of The University Of California Engineered polymerase for threose nucleic acid synthesis

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108795900B (zh) * 2017-04-27 2021-02-02 深圳华大智造科技股份有限公司 Dna聚合酶及其制备方法
CN108795900A (zh) * 2017-04-27 2018-11-13 深圳华大智造科技有限公司 Dna聚合酶及其制备方法
CN112639089A (zh) * 2018-09-03 2021-04-09 深圳华大生命科学研究院 重组型kod聚合酶
WO2020048329A1 (zh) * 2018-09-03 2020-03-12 深圳华大生命科学研究院 重组型kod聚合酶
CN112585264A (zh) * 2018-09-03 2021-03-30 深圳华大生命科学研究院 重组型kod聚合酶
WO2020047695A1 (zh) * 2018-09-03 2020-03-12 深圳华大生命科学研究院 重组型kod聚合酶
CN112639089B (zh) * 2018-09-03 2024-02-09 深圳华大生命科学研究院 重组型kod聚合酶
US12168785B2 (en) 2018-09-03 2024-12-17 Bgi Shenzhen Recombinant KOD polymerase
CN112639090A (zh) * 2018-12-05 2021-04-09 亿明达股份有限公司 聚合酶、组合物及使用方法
CN114846149A (zh) * 2019-12-23 2022-08-02 陈呈尧 用于不依赖于模板的核酸合成的方法和试剂盒
WO2022082482A1 (zh) * 2020-10-21 2022-04-28 深圳华大生命科学研究院 重组型kod聚合酶
CN116286720A (zh) * 2023-03-29 2023-06-23 赛纳生物科技(北京)有限公司 一种dna聚合酶
WO2025260380A1 (zh) * 2024-06-21 2025-12-26 华大工程生物学长荡湖研究所 重组型kod聚合酶

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CA2951416A1 (en) 2015-12-30
AU2021290289B2 (en) 2024-02-15
US10870836B2 (en) 2020-12-22
EP3702471C0 (en) 2025-12-24
ES3060591T3 (en) 2026-03-27
DK3161154T3 (da) 2020-06-15
WO2015200693A1 (en) 2015-12-30
EP3702471B1 (en) 2025-12-24
AU2015279794B2 (en) 2021-09-30
EP3161154B1 (en) 2020-04-15
US20170355970A1 (en) 2017-12-14
US9765309B2 (en) 2017-09-19
US20210246435A1 (en) 2021-08-12
EP3702471A1 (en) 2020-09-02
EP3161154A1 (en) 2017-05-03
US12110514B2 (en) 2024-10-08
JP2020127415A (ja) 2020-08-27
JP2022033882A (ja) 2022-03-02
JP2017518750A (ja) 2017-07-13
US20150376582A1 (en) 2015-12-31
JP6737710B2 (ja) 2020-08-12
HK1231517A1 (en) 2017-12-22
AU2021290289A1 (en) 2022-01-27
AU2015279794A1 (en) 2016-12-22
ES2788949T3 (es) 2020-10-23
PL3161154T3 (pl) 2020-10-19
PT3161154T (pt) 2020-06-08

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