CN106536728A - 用于核苷酸类似物的改进的掺入的修饰的聚合酶 - Google Patents

用于核苷酸类似物的改进的掺入的修饰的聚合酶 Download PDF

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Publication number
CN106536728A
CN106536728A CN201580033818.6A CN201580033818A CN106536728A CN 106536728 A CN106536728 A CN 106536728A CN 201580033818 A CN201580033818 A CN 201580033818A CN 106536728 A CN106536728 A CN 106536728A
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China
Prior art keywords
polymerase
transformation
polymerases
functionally equivalent
nucleotide
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CN201580033818.6A
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Chinese (zh)
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陈呈尧
艾琳·博马蒂
莫利·何
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Illumina Inc
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Illumina Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580033818.6A 2014-06-27 2015-06-25 用于核苷酸类似物的改进的掺入的修饰的聚合酶 Pending CN106536728A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462018470P 2014-06-27 2014-06-27
US62/018,470 2014-06-27
PCT/US2015/037785 WO2015200693A1 (en) 2014-06-27 2015-06-25 Modified polymerases for improved incorporation of nucleotide analogues

Publications (1)

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CN106536728A true CN106536728A (zh) 2017-03-22

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CN201580033818.6A Pending CN106536728A (zh) 2014-06-27 2015-06-25 用于核苷酸类似物的改进的掺入的修饰的聚合酶

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US (3) US9765309B2 (cg-RX-API-DMAC7.html)
EP (2) EP3161154B1 (cg-RX-API-DMAC7.html)
JP (3) JP6737710B2 (cg-RX-API-DMAC7.html)
CN (1) CN106536728A (cg-RX-API-DMAC7.html)
AU (2) AU2015279794B2 (cg-RX-API-DMAC7.html)
DK (1) DK3161154T3 (cg-RX-API-DMAC7.html)
ES (1) ES2788949T3 (cg-RX-API-DMAC7.html)
PL (1) PL3161154T3 (cg-RX-API-DMAC7.html)
PT (1) PT3161154T (cg-RX-API-DMAC7.html)
WO (1) WO2015200693A1 (cg-RX-API-DMAC7.html)

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CN108795900A (zh) * 2017-04-27 2018-11-13 深圳华大智造科技有限公司 Dna聚合酶及其制备方法
WO2020048329A1 (zh) * 2018-09-03 2020-03-12 深圳华大生命科学研究院 重组型kod聚合酶
CN112639090A (zh) * 2018-12-05 2021-04-09 亿明达股份有限公司 聚合酶、组合物及使用方法
WO2022082482A1 (zh) * 2020-10-21 2022-04-28 深圳华大生命科学研究院 重组型kod聚合酶
CN114846149A (zh) * 2019-12-23 2022-08-02 陈呈尧 用于不依赖于模板的核酸合成的方法和试剂盒
CN116286720A (zh) * 2023-03-29 2023-06-23 赛纳生物科技(北京)有限公司 一种dna聚合酶

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CN112673098B (zh) 2018-10-31 2023-01-06 亿明达股份有限公司 聚合酶、组合物及使用方法
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
AU2020352503A1 (en) 2019-09-23 2022-05-19 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
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US20230203592A1 (en) 2020-05-05 2023-06-29 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
KR20240024924A (ko) 2021-06-29 2024-02-26 애질런트 테크놀로지스, 인크. 폴리머라제 돌연변이체 및 3'-oh 비차단 가역적 종결자와의 사용
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
TWI869710B (zh) 2021-09-29 2025-01-11 呈堯 陳 用於無模板ｒｎａ合成的核酸聚合酶變體、試劑套組及方法
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
WO2024155578A1 (en) * 2023-01-16 2024-07-25 The Regents Of The University Of California Engineered polymerase for threose nucleic acid synthesis

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108795900A (zh) * 2017-04-27 2018-11-13 深圳华大智造科技有限公司 Dna聚合酶及其制备方法
CN108795900B (zh) * 2017-04-27 2021-02-02 深圳华大智造科技股份有限公司 Dna聚合酶及其制备方法
WO2020048329A1 (zh) * 2018-09-03 2020-03-12 深圳华大生命科学研究院 重组型kod聚合酶
WO2020047695A1 (zh) * 2018-09-03 2020-03-12 深圳华大生命科学研究院 重组型kod聚合酶
CN112585264A (zh) * 2018-09-03 2021-03-30 深圳华大生命科学研究院 重组型kod聚合酶
CN112639089A (zh) * 2018-09-03 2021-04-09 深圳华大生命科学研究院 重组型kod聚合酶
CN112639089B (zh) * 2018-09-03 2024-02-09 深圳华大生命科学研究院 重组型kod聚合酶
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CN112639090A (zh) * 2018-12-05 2021-04-09 亿明达股份有限公司 聚合酶、组合物及使用方法
CN114846149A (zh) * 2019-12-23 2022-08-02 陈呈尧 用于不依赖于模板的核酸合成的方法和试剂盒
WO2022082482A1 (zh) * 2020-10-21 2022-04-28 深圳华大生命科学研究院 重组型kod聚合酶
CN116286720A (zh) * 2023-03-29 2023-06-23 赛纳生物科技(北京)有限公司 一种dna聚合酶

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EP3702471A1 (en) 2020-09-02
AU2021290289A1 (en) 2022-01-27
US9765309B2 (en) 2017-09-19
ES2788949T3 (es) 2020-10-23
US12110514B2 (en) 2024-10-08
AU2015279794A1 (en) 2016-12-22
JP6737710B2 (ja) 2020-08-12
US20150376582A1 (en) 2015-12-31
EP3161154B1 (en) 2020-04-15
PL3161154T3 (pl) 2020-10-19
US20170355970A1 (en) 2017-12-14
JP2022033882A (ja) 2022-03-02
US10870836B2 (en) 2020-12-22
JP2017518750A (ja) 2017-07-13
HK1231517A1 (en) 2017-12-22
PT3161154T (pt) 2020-06-08
CA2951416A1 (en) 2015-12-30
AU2021290289B2 (en) 2024-02-15
EP3702471B1 (en) 2025-12-24
AU2015279794B2 (en) 2021-09-30
EP3161154A1 (en) 2017-05-03
JP2020127415A (ja) 2020-08-27
US20210246435A1 (en) 2021-08-12
DK3161154T3 (da) 2020-06-15
WO2015200693A1 (en) 2015-12-30

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