CN106525836A - Detection method and detection card for macrodantin metabolite in milk products - Google Patents
Detection method and detection card for macrodantin metabolite in milk products Download PDFInfo
- Publication number
- CN106525836A CN106525836A CN201611112545.7A CN201611112545A CN106525836A CN 106525836 A CN106525836 A CN 106525836A CN 201611112545 A CN201611112545 A CN 201611112545A CN 106525836 A CN106525836 A CN 106525836A
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- detection
- card
- cistofuran metabolite
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
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- Chemical & Material Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to the technical field of food detection, and discloses a detection card for macrodantin metabolite in milk products. The detection card comprises a card strip; a sample adding hole is formed in the surface of the card strip, a sealing strip is pasted on the sample adding hole, a detection device located inside a detection window is communicated to the bottom end of the sample adding hole, and comprises a detection strip, and a detection line and a quality control line are arranged on the upper surface of the detection strip. According to a detection method and the detection card for the macrodantin metabolite in milk products, the sample adding hole and the sealing strip are arranged, experimenters dropwise add treated to-be-detected liquid into the detection card through the sample adding hole, it is guaranteed that the to-be-detected liquid rapidly enters the detection card, the performance of the to-be-detected liquid is guaranteed, and therefore the detection effects of the detection method and detection card for the macrodantin metabolite in milk products are guaranteed; as the sealing strip is pasted on the sample adding hole, it is guaranteed that when the detection card for the macrodantin metabolite in milk products is stored, the detection device cannot be polluted by the outside world.
Description
Technical field
The present invention relates to technical field of food detection, in specially a kind of milk the detection method of Cistofuran metabolite and
Detection card.
Background technology
Nitrofurantoin is a kind of common synthetic antibiotic, because of its fabulous antibiotic property and pharmacokinetic properties, normal quilt
For links such as animal feeding and circulations.This kind of material be also often taken as starter or fattening agent be used for pig, poultry, milch cow with
And in the raising of Fish.Long-term zoopery shows that nitrofurantoin and its metabolite are respectively provided with carcinogenecity and cause mutagenicity.
Therefore, nitrofurantoin is inhibited on consumption letting animals feed and uses.But because nitrofurantoin is in treatment enterobacterial infection etc.
Aspect curative effect is obvious and cheap, also there is illegal use, if long-term consumption contains nitrofurantoin in milk cattle cultivating
During the milk that the Lac Bovis seu Bubali of the milch cow output of composition feedstuff is processed into comes into the market, can cause very tight to the healthy of drinking person
The impact of weight, for this purpose, we have proposed the detection method and detection card of Cistofuran metabolite in a milk, is easy to food to defend
Raw superintendent office carries out the detection of Cistofuran metabolite to milk, ensures the safe diet of people.
The content of the invention
（One）The technical problem of solution
For the deficiencies in the prior art, the invention provides the detection method of Cistofuran metabolite and detection in a kind of milk
Card, possesses the advantage detected for Cistofuran metabolite content in milk, solves existing equipment and be difficult to in milk
The problem detected by Cistofuran metabolite content.
（Two）Technical scheme
To realize the above-mentioned purpose detected for Cistofuran metabolite content in milk, the present invention provides following technical side
Case：In a kind of milk, the detection method of Cistofuran metabolite, comprises the steps：
1）The packaging of testing sample is opened, and 2 grams of testing sample is extracted using syringe, if cannot carry out in time to testing sample
Detection, then be stored in testing sample in freezing environment；
2）2 grams of testing sample is added into centrifuge tube using syringe, hydrochloric acid is added in centrifuge tube, shake uniform 3-5
Minute, centrifugal treating is carried out, the supernatant is taken and is given over to next step process use；
3）After the supernatant is taken out, sodium hydroxide is added, shake uniform 1-3 minutes, derived in water-bath or air bath；
4）After the completion of derivative, the derivative liquid of cooling hydrolysis adjusts the pH value of the derivative liquid of hydrolysis to neutrality；
5）Taking out derivative liquid to centrifuge carries out centrifugal treating, obtains the supernatant to be measured；
6）The 0.5ml supernatantes are drawn in enzyme mark hole, 1-3 minutes are shaken, red material dissolving in enzyme mark hole disappears, and waits 3-
5 minutes, all solution in enzyme mark hole are drawn using syringe；
7）The sealing strip torn on detection card, the solution in syringe is added drop-wise in the well of detection card, timing is carried out；
8）After timing 3-5 minutes, the depth degree of detection line and nature controlling line in observation detection window, if detection line compares nature controlling line
Deep, then Cistofuran metabolite is feminine gender, if detection line is more shallow than nature controlling line, Cistofuran metabolite is the positive, if observation is not
Go out nature controlling line, then illustrate this detection failure, repeat the above steps are detected again.
Preferably, the step 1）In freezing environment temperature be -20 DEG C.
Preferably, the step 4）In the derivative liquid of cooling hydrolysis to after less than 15 DEG C, start to adjust hydrolysis and derive liquid
PH value.
Preferably, the step 3）In water-bath temperature range at 90-99 DEG C, the temperature range of air bath is in 90-104
℃。
Preferably, the step 3）The scope of the sodium hydroxide capacity of middle addition is in 0.3-0.5ml.
Preferably, the step 2）The scope of the salt acid capacity of middle addition is in 0.5ml-1ml.
Preferably, the step 7）In timing time be 3-5 minutes.
Preferably, the step 3）In the derivative time be 30-60 minutes.
The detection card of Cistofuran metabolite in a kind of milk, including card article, the surface of the card article offer well,
Sealing strip is pasted with above the well, the bottom of the well is communicated with the detection means inside detection window,
The detection means includes detector bar, and the upper surface of the detector bar is provided with detection line and nature controlling line.
Preferably, size of the size of the sealing strip more than well.
（Three）Beneficial effect
Compared with prior art, the invention provides in a kind of milk the detection method of Cistofuran metabolite and detection card, tool
Standby following beneficial effect：
1st, the detection method of Cistofuran metabolite and detection card in the milk, by arranging detection means, it would be desirable to examined
The testing liquid of survey is flowed in detection means, and testing liquid is reacted with the detector bar in detection means, waits 3-5 minutes
Afterwards, if detected successfully, detection line and nature controlling line on detector bar, occurs, by comparison and detection line and the depth of nature controlling line
Degree, judges to Cistofuran metabolite, if detection line is than nature controlling line depth, Cistofuran metabolite is feminine gender, if inspection
Survey line is more shallow than nature controlling line, then Cistofuran metabolite is the positive, if detection failure, after 3-5 minutes are waited, on detector bar
Nature controlling line cannot be shown, experimenter will be detected to Cistofuran metabolite in milk again, reached it is simple to operate,
The effect detected by the convenient and swift Cistofuran metabolite in milk, substantially increases nitrofurantoin metabolism in the milk
The practicality of the detection method and detection card of thing, being convenient to Food Hygiene Surveillance department carries out Cistofuran metabolite to milk
Detection, ensures the safe diet of people.
2nd, the detection method of Cistofuran metabolite and detection card in the milk, by arranging well and sealing strip, real
Test personnel treated testing liquid is dropped in detection card from well, it is ensured that testing liquid rapidly enters detection card
In, it is ensured that the property of medicine of testing liquid, so as to ensure that the detection method of Cistofuran metabolite in the milk and detection card
Detection results, sealing strip are pasted onto on well, it is ensured that the detection of Cistofuran metabolite in the milk is stuck in and is preserved
When detection means by outside contamination, will not extend Cistofuran metabolite in the milk detection card useful life, so as to
Improve the practicality of the detection method and detection card of Cistofuran metabolite in the milk.
Description of the drawings
Fig. 1 is Cistofuran metabolite detection card structure schematic diagram in a kind of milk of the invention.
In figure：1 card article, 2 wells, 3 sealing strips, 4 detection windows, 5 detection means, 51 detector bars, 52 detection lines, 53 Quality Controls
Line.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Fig. 1 is referred to, the detection method of Cistofuran metabolite, comprises the steps in a kind of milk：
1）The packaging of testing sample is opened, and 2 grams of testing sample is extracted using syringe, if cannot carry out in time to testing sample
Detection, then be stored in testing sample in -20 DEG C of freezing environment；
2）2 grams of testing sample is added into centrifuge tube using syringe, the hydrochloric acid of 0.5ml-1ml is added in centrifuge tube,
Uniform 3-5 minutes are shaken, centrifugal treating is carried out, the supernatant is taken and is given over to next step process use；
3）After the supernatant is taken out, add 0.3-0.5ml sodium hydroxide, shake uniform 1-3 minutes, in 90-99 DEG C of water-bath or
30-60 minutes derived from 90-104 DEG C of air bath；
4）After the completion of derivative, the derivative liquid of cooling hydrolysis adjusts the pH value of the derivative liquid of hydrolysis to neutrality to less than 15 DEG C；
5）Taking out derivative liquid to centrifuge carries out centrifugal treating, obtains the supernatant to be measured；
6）The 0.5ml supernatantes are drawn in enzyme mark hole, 1-3 minutes are shaken, red material dissolving in enzyme mark hole disappears, and waits 3-
5 minutes, all solution in enzyme mark hole are drawn using syringe；
7）The sealing strip torn on detection card, the solution in syringe is added drop-wise in the well of detection card, timing is carried out；
8）After timing 3-5 minutes, the depth degree of detection line and nature controlling line in observation detection window, if detection line compares nature controlling line
Deep, then Cistofuran metabolite is feminine gender, if detection line is more shallow than nature controlling line, Cistofuran metabolite is the positive, if observation is not
Go out nature controlling line, then illustrate this detection failure, repeat the above steps are detected again.
The detection card of Cistofuran metabolite in a kind of milk, including card article 1, the surface of card article 1 offer well 2,
The top of well 2 is pasted with sealing strip 3, the size of the size of sealing strip 3 more than well 2, by arranging well 2 and close
Treated testing liquid is dropped in detection card by strip of paper used for sealing 3, experimenter from well 2, it is ensured that testing liquid is quick
Into in detection card, it is ensured that the property of medicine of testing liquid, so as to ensure that the detection method of Cistofuran metabolite in the milk
And the Detection results for blocking are detected, sealing strip 3 is pasted onto on well 2, it is ensured that the detection of Cistofuran metabolite in the milk
Being stuck in when being preserved detection means and by outside contamination, will not extending the detection card of Cistofuran metabolite in the milk makes
With the time limit, so as to improve the practicality of the detection method of Cistofuran metabolite and detection card in the milk, the bottom of well 2
End is communicated with the detection means 5 inside detection window 4, by arranging detection window 4, is easy to experimenter's observation testing result,
Detection means 5 includes detector bar 51, and the upper surface of detector bar 51 is provided with detection line 52 and nature controlling line 53, by arranging detection dress
Put 5, it would be desirable to which the testing liquid for being detected is flowed in detection means 5, and testing liquid is entered with the detector bar 51 in detection means 5
Row reaction, after waiting 3-5 minutes, if detected successfully, occurs detection line 52 and nature controlling line 53, by right on detector bar 51
Than detection line 52 and the depth degree of nature controlling line 53, Cistofuran metabolite is judged, if detection line 52 compares nature controlling line
53 is deep, then Cistofuran metabolite is feminine gender, if detection line 52 is more shallow than nature controlling line 53, Cistofuran metabolite is the positive, such as
Fruit detection failure, then, after 3-5 minutes are waited, cannot show nature controlling line 53 on detector bar 51, experimenter will again to breast
In product, Cistofuran metabolite is detected, has reached simple to operate, and the convenient and swift Cistofuran metabolite in milk enters
The effect of row detection, substantially increases the practicality of the detection method and detection card of Cistofuran metabolite in the milk, convenient
The detection of Cistofuran metabolite is carried out to milk in Food Hygiene Surveillance department, the safe diet of people is ensured.
In the milk, the detection card of Cistofuran metabolite when in use, is torn and detects the sealing strip 3 on blocking, by syringe
Interior solution is added drop-wise in the well 2 of detection card, and operator will detect card slant setting, and angle of inclination is 30 degree, is easy to
Solution is rapidly introduced in detection means 5, stands 3-5 minutes, by observing the detection line 52 and nature controlling line 53 detected in window 4
Shade degree.
In sum, the detection method of Cistofuran metabolite and detection card in the milk, by arranging detection means 5,
The testing liquid for needing to be detected is flowed in detection means 5, testing liquid is carried out instead with the detector bar 51 in detection means 5
Should, after waiting 3-5 minutes, if detected successfully, detection line 52 and nature controlling line 53 on detector bar 51, occurs, by contrasting inspection
The depth degree of survey line 52 and nature controlling line 53, judges to Cistofuran metabolite, if detection line 52 is deeper than nature controlling line 53,
Then Cistofuran metabolite is feminine gender, if detection line 52 is more shallow than nature controlling line 53, Cistofuran metabolite is the positive, if detection
Failure, then, after 3-5 minutes are waited, cannot show nature controlling line 53 on detector bar 51, experimenter will again to furan in milk
Mutter appropriate because metabolite is detected, reached simple to operate, the convenient and swift Cistofuran metabolite in milk is detected
Effect, substantially increase Cistofuran metabolite in the milk detection method and detection card practicality, be convenient to food
Health supervision department carries out the detection of Cistofuran metabolite to milk, ensures the safe diet of people.
In the milk, the detection method of Cistofuran metabolite and detection card, by arranging well 2 and sealing strip 3, real
Test personnel treated testing liquid is dropped in detection card from well 2, it is ensured that testing liquid rapidly enters detection
In card, it is ensured that the property of medicine of testing liquid, so as to ensure that the detection method of Cistofuran metabolite and detection card in the milk
Detection results, sealing strip 3 is pasted onto on well 2, it is ensured that in the milk, the detection of Cistofuran metabolite is stuck in is carried out
During preservation, detection means by outside contamination, will not extend the useful life of the detection card of Cistofuran metabolite in the milk,
So as to improve the practicality of the detection method of Cistofuran metabolite and detection card in the milk.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation are made a distinction with another entity or operation, and are not necessarily required or implied these entities or deposit between operating
In any this actual relation or order.And, term " including ", "comprising" or its any other variant are intended to
Nonexcludability is included, so that a series of process, method, article or equipment including key elements not only will including those
Element, but also including other key elements being not expressly set out, or also include for this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions, the key element for being limited by sentence "including a ...", it is not excluded that
Also there is other identical element in process, method, article or equipment including the key element.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding can carry out various changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (10)
1. in a kind of milk Cistofuran metabolite detection method, it is characterised in that comprise the steps：
1）The packaging of testing sample is opened, and 2 grams of testing sample is extracted using syringe, if cannot carry out in time to testing sample
Detection, then be stored in testing sample in freezing environment；
2）2 grams of testing sample is added into centrifuge tube using syringe, hydrochloric acid is added in centrifuge tube, shake uniform 3-5
Minute, centrifugal treating is carried out, the supernatant is taken and is given over to next step process use；
3）After the supernatant is taken out, sodium hydroxide is added, shake uniform 1-3 minutes, derived in water-bath or air bath；
4）After the completion of derivative, the derivative liquid of cooling hydrolysis adjusts the pH value of the derivative liquid of hydrolysis to neutrality；
5）Taking out derivative liquid to centrifuge carries out centrifugal treating, obtains the supernatant to be measured；
6）The 0.5ml supernatantes are drawn in enzyme mark hole, 1-3 minutes are shaken, red material dissolving in enzyme mark hole disappears, and waits 3-
5 minutes, all solution in enzyme mark hole are drawn using syringe；
7）The sealing strip torn on detection card, the solution in syringe is added drop-wise in the well of detection card, timing is carried out；
8）After the completion of timing, the depth degree of detection line and nature controlling line in observation detection window, if detection line is than nature controlling line depth,
Cistofuran metabolite is feminine gender, if detection line is more shallow than nature controlling line, Cistofuran metabolite is the positive, if can not observe Quality Control
Line, then illustrate this detection failure, and repeat the above steps are detected again.
2. in a kind of milk according to claim 1 Cistofuran metabolite detection method, it is characterised in that：The step
Rapid 1）In freezing environment temperature be -20 DEG C.
3. in a kind of milk according to claim 1 Cistofuran metabolite detection method, it is characterised in that：The step
Rapid 4）In the derivative liquid of cooling hydrolysis to after less than 15 DEG C, start to adjust the pH value that hydrolysis derives liquid.
4. in a kind of milk according to claim 1 Cistofuran metabolite detection method, it is characterised in that：The step
Rapid 3）In water-bath temperature range at 90-99 DEG C, the temperature range of air bath is at 90-104 DEG C.
5. in a kind of milk according to claim 1 Cistofuran metabolite detection method, it is characterised in that：The step
Rapid 3）The scope of the sodium hydroxide capacity of middle addition is in 0.3-0.5ml.
6. in a kind of milk according to claim 1 Cistofuran metabolite detection method, it is characterised in that：The step
Rapid 2）The scope of the salt acid capacity of middle addition is in 0.5ml-1ml.
7. in a kind of milk according to claim 1 Cistofuran metabolite detection method, it is characterised in that：The step
Rapid 7）In timing time be 3-5 minutes.
8. in a kind of milk according to claim 1 Cistofuran metabolite detection method, it is characterised in that：The step
Rapid 3）In the derivative time be 30-60 minutes.
9. in a kind of milk Cistofuran metabolite detection card, including card article（1）, the card article（1）Surface offer plus
Sample hole（2）, it is characterised in that the well（2）Top be pasted with sealing strip（3）, the well（2）Bottom connection
Have positioned at detection window（4）Internal detection means（5）, the detection means（5）Including detector bar（51）, the detector bar（51）
Upper surface be provided with detection line（52）And nature controlling line（53）.
10. in a kind of milk according to claim 9 Cistofuran metabolite detection card, it is characterised in that：It is described close
Strip of paper used for sealing（3）Size be more than well（2）Size.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611112545.7A CN106525836A (en) | 2016-12-07 | 2016-12-07 | Detection method and detection card for macrodantin metabolite in milk products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611112545.7A CN106525836A (en) | 2016-12-07 | 2016-12-07 | Detection method and detection card for macrodantin metabolite in milk products |
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| Publication Number | Publication Date |
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| CN106525836A true CN106525836A (en) | 2017-03-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611112545.7A Pending CN106525836A (en) | 2016-12-07 | 2016-12-07 | Detection method and detection card for macrodantin metabolite in milk products |
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|---|---|---|---|---|
| US20130065321A1 (en) * | 2011-09-09 | 2013-03-14 | Church & Dwight Co., Inc. | Systems, methods, and test kits for analyte variation detection |
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| CN205280729U (en) * | 2015-12-29 | 2016-06-01 | 北京索莱宝科技有限公司 | Mycoplasma detect reagent box |
| DE202015104611U1 (en) * | 2015-08-31 | 2016-08-25 | R-Biopharm Ag | Test strip cover |
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2016
- 2016-12-07 CN CN201611112545.7A patent/CN106525836A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130065321A1 (en) * | 2011-09-09 | 2013-03-14 | Church & Dwight Co., Inc. | Systems, methods, and test kits for analyte variation detection |
| CN203350257U (en) * | 2013-06-27 | 2013-12-18 | 北京华安麦科生物技术有限公司 | Rapid detection kit for nitrofurans drug metabolites by colloidal gold |
| CN104215765A (en) * | 2014-09-13 | 2014-12-17 | 佛山市质量计量监督检测中心 | Preparation of immunizing antigen and envelope antigen for detecting nitrofurantoin metabolites |
| CN104374910A (en) * | 2014-11-19 | 2015-02-25 | 无锡中德伯尔生物技术有限公司 | Kit and method applied to detection of nitrofurans drug metabolite |
| CN204405674U (en) * | 2014-12-18 | 2015-06-17 | 杭州隆基生物技术有限公司 | A kind of stomach Helicobacter pylori test card |
| CN204405675U (en) * | 2014-12-18 | 2015-06-17 | 杭州隆基生物技术有限公司 | A kind of mycoplasma pneumoniae IgM antibody test card |
| DE202015104611U1 (en) * | 2015-08-31 | 2016-08-25 | R-Biopharm Ag | Test strip cover |
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