CN107164232A - The compound micro-ecological preparation formula and preparation method fermented available for field - Google Patents
The compound micro-ecological preparation formula and preparation method fermented available for field Download PDFInfo
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- CN107164232A CN107164232A CN201710591746.8A CN201710591746A CN107164232A CN 107164232 A CN107164232 A CN 107164232A CN 201710591746 A CN201710591746 A CN 201710591746A CN 107164232 A CN107164232 A CN 107164232A
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- lactic acid
- enterococcus faecalis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
A kind of compound micro-ecological preparation formula fermented available for field, it is related to bioengineering field, the formula is by the cfu/g of bacillus subtilis 5 15 hundred million, the cfu/g of lactic acid enterococcus faecalis 20 50 hundred million, the 200g/Kg of Dextrose Monohydrate 100, the 500g/Kg of the chaff 350 and 350g/Kg of zeolite powder 250 that unites is constituted, the invention has the advantages that lactic acid enterococcus faecalis can simply be spread cultivation out Come under more severe breeding environment in the wild, and preserve the long period, there is provided convenient for raiser, cheap probiotics is used for regulating water quality and aquatic economic animal intestinal health, spread cultivation easy long mould with field before, it is not save-resistant to compare, with revolutionary advantage.
Description
Technical field：
The present invention relates to bioengineering field, and in particular to a kind of compound micro-ecological preparation formula fermented available for field
And preparation method.
Background technology：
The history of application of the probiotics in aquaculture more than 20 years, its course is about photosynthetic bacteria, bud
Spore bacillus, saccharomycete, EM bacterium, lactic acid bacteria.In recent years, anti-cry is prohibited with the continuous improvement and globalization of aquaculture density
More and more strong, regulating water quality and the enteron aisle effect of probiotics are more and more obvious.Lactic acid bacteria is de- as the new flora of a class
Grain husk and go out, wherein again it is the most characteristic with the function of enterococcus faecalis.During the use of lactic acid bacteria, the greatest problem run into is
The not save-resistant and field fermentation of strain is unsuccessful, difficulty that enterococcus faecalis is not save-resistant and field fermentation spreads cultivation, on solving
Technical problem is stated, spy proposes a kind of new technical scheme.
The content of the invention：
The invention aims to overcome the weak point that above-mentioned prior art is present, and provide a kind of available for field
The compound micro-ecological preparation formula and preparation method of fermentation.
The technical solution adopted by the present invention is：A kind of compound micro-ecological preparation formula fermented available for field, its feature
It is：The formula is by the cfu/g of bacillus subtilis 5-15 hundred million, the cfu/g of lactic acid enterococcus faecalis 20-50 hundred million, Dextrose Monohydrate 100-
200g/Kg, system chaff 350-500g/Kg and zeolite powder 250-350g/Kg compositions.
Described lactic acid enterococcus faecalis is screened by screening washer, and choosing can survive much in 20 DEG C of preservation at room temperature
In the strain of 1 month.
The preparation method of described lactic acid enterococcus faecalis is as follows, chooses one plant of breast of screening in the cultured prawn enteron aisle of South Sea waters
Sour enterococcus faecalis, one plant of lactic acid enterococcus faecalis is first carried out processing is dried again after freezing processing, freezing processing, does it
Into bacterium powder shape, and bacterium powder is done into material using phosphatide be coated with, the bacterium powder that completes will be coated with and keep two under conditions of 20 DEG C
Year, and sampling observation month by month is carried out during preservation, in real time the survival rate of monitoring lactic acid enterococcus faecalis.
A kind of preparation method of the compound micro-ecological preparation formula fermented available for field, this method is by compounding withered grass bud
Spore bacillus, lactic acid enterococcus faecalis and culture medium, bacillus subtilis provide the environment of relative anaerobism, culture for lactic acid enterococcus faecalis
Cellulose family and other macromolecular components in base, are utilized for lactic acid Enterococcus faecalis fermentation.
The formula of described culture medium is by yeast hydrolyate 100-200g/Kg, Dextrose Monohydrate 200-300g/Kg, yellow corruption
Sour potassium 100-150g/Kg, system chaff 200-300g/Kg and zeolite powder 100-200g/Kg compositions.
A kind of verification method of the compound micro-ecological preparation formula fermented available for field, this method uses orthogonal test pair
Probiotics formula carries out screening verification, and the factor 1 is bacillus subtilis bacteria concentration, and the factor 2 is lactic acid enterococcus faecalis concentration, because
Son 3 is culture medium concentration, monitors the concentration of lactic acid enterococcus faecalis in liquid after fermentation, and organic acid concentration is filtered out most suitable
Probiotics is formulated and culture medium prescription.
Described culture medium prescription is by yeast hydrolyate 100-200g/Kg, Dextrose Monohydrate 200-300g/Kg, fulvic acid
Potassium 100-150g/Kg, system chaff 200-300g/Kg and zeolite powder 100-200g/Kg compositions.
Beneficial effects of the present invention are：It is tired that on the one hand this technology solves that enterococcus faecalis is not save-resistant and field fermentation spreads cultivation
Difficult the problem of.In terms of enterococcus faecalis preservation is solved the problems, such as, this technology is mainly characterized by screening holding time length at normal temperatures
Strain and solved using strain passivating technique；Field fermentation problem is then by being prepared into compound micro-ecological preparation, to improve
The life span of enterococcus faecalis is solved with excellent living space is created.
Embodiment：
The present invention is further described in conjunction with specific embodiments：
Embodiment one：
A kind of compound micro-ecological preparation formula fermented available for field, it is characterised in that：The formula is by bacillus subtilis
The cfu/g of bacterium 500,000,000, the cfu/g of lactic acid enterococcus faecalis 2,000,000,000, Dextrose Monohydrate 100/Kg, system chaff 350/Kg and zeolite powder 250/Kg groups
Into.
Described lactic acid enterococcus faecalis is screened by screening washer, and choosing can survive much in 20 DEG C of preservation at room temperature
In the strain of 1 month.
The preparation method of described lactic acid enterococcus faecalis is as follows, chooses one plant of breast of screening in the cultured prawn enteron aisle of South Sea waters
Sour enterococcus faecalis, one plant of lactic acid enterococcus faecalis is first carried out processing is dried again after freezing processing, freezing processing, does it
Into bacterium powder shape, and bacterium powder is done into material using phosphatide be coated with, the bacterium powder that completes will be coated with and keep two under conditions of 20 DEG C
Year, and sampling observation month by month is carried out during preservation, in real time the survival rate of monitoring lactic acid enterococcus faecalis.
Embodiment two：
A kind of compound micro-ecological preparation formula fermented available for field, it is characterised in that：The formula is by bacillus subtilis
The cfu/g of bacterium 1,000,000,000, the cfu/g of lactic acid enterococcus faecalis 3,500,000,000, Dextrose Monohydrate 150g/Kg, system chaff 400g/Kg and zeolite powder 300/Kg
Composition.
Described lactic acid enterococcus faecalis is screened by screening washer, and choosing can survive much in 20 DEG C of preservation at room temperature
In the strain of 1 month.
The preparation method of described lactic acid enterococcus faecalis is as follows, chooses one plant of breast of screening in the cultured prawn enteron aisle of South Sea waters
Sour enterococcus faecalis, one plant of lactic acid enterococcus faecalis is first carried out processing is dried again after freezing processing, freezing processing, does it
Into bacterium powder shape, and bacterium powder is done into material using phosphatide be coated with, the bacterium powder that completes will be coated with and keep two under conditions of 20 DEG C
Year, and sampling observation month by month is carried out during preservation, in real time the survival rate of monitoring lactic acid enterococcus faecalis.
Embodiment three：
A kind of compound micro-ecological preparation formula fermented available for field, it is characterised in that：The formula is by bacillus subtilis
The cfu/g of bacterium 1,500,000,000, the cfu/g of lactic acid enterococcus faecalis 5,000,000,000, Dextrose Monohydrate 200g/Kg, system chaff 500g/Kg and zeolite powder 350g/
Kg is constituted.
Described lactic acid enterococcus faecalis is screened by screening washer, and choosing can survive much in 20 DEG C of preservation at room temperature
In the strain of 1 month.
The preparation method of described lactic acid enterococcus faecalis is as follows, chooses one plant of breast of screening in the cultured prawn enteron aisle of South Sea waters
Sour enterococcus faecalis, one plant of lactic acid enterococcus faecalis is first carried out processing is dried again after freezing processing, freezing processing, does it
Into bacterium powder shape, and bacterium powder is done into material using phosphatide be coated with, the bacterium powder that completes will be coated with and keep two under conditions of 20 DEG C
Year, and sampling observation month by month is carried out during preservation, in real time the survival rate of monitoring lactic acid enterococcus faecalis.
Three above embodiment is prepared using following preparation method：
A kind of preparation method of the compound micro-ecological preparation formula fermented available for field, this method is by compounding withered grass bud
Spore bacillus, lactic acid enterococcus faecalis and culture medium, bacillus subtilis provide the environment of relative anaerobism, culture for lactic acid enterococcus faecalis
Cellulose family and other macromolecular components in base, are utilized for lactic acid Enterococcus faecalis fermentation.
The formula of described culture medium is by yeast hydrolyate 150g/Kg, Dextrose Monohydrate 250g/Kg, potassium fulvate 130g/
Kg, system chaff 250g/Kg and zeolite powder 150g/Kg compositions.
Three above embodiment is verified using following methods：
A kind of verification method of the compound micro-ecological preparation formula fermented available for field, this method uses orthogonal test pair
Probiotics formula carries out screening verification, and the factor 1 is bacillus subtilis bacteria concentration, and the factor 2 is lactic acid enterococcus faecalis concentration, because
Son 3 is culture medium concentration, monitors the concentration of lactic acid enterococcus faecalis in liquid after fermentation, and organic acid concentration is filtered out most suitable
Probiotics is formulated and culture medium prescription.
Using effect is tested：
This research is continuously on probation 1 year by 100 enterprises, investigates the effect that the compound micro-ecological preparation is formulated each side.
Test event standards of grading are：Very satisfied 5 points, 4 points are satisfied with, general 3 points, 2 points, very dissatisfied 1 point are unsatisfied with.It is wherein general
Logical group is common probiotics formula；
The invention has the advantages that lactic acid enterococcus faecalis can simply spread cultivation out Come under more severe breeding environment in the wild, and
The long period is preserved, providing convenient, cheap probiotics for raiser is used for regulating water quality and aquatic economic animal enteron aisle
Health, with field before spread cultivation easy long mould, it is not save-resistant compared with, with revolutionary advantage.
For those skilled in the art, other can be made various corresponding according to above technical scheme and design
Change and deformation, and all these change and deformation should all belong within the protection domain of the claims in the present invention.
Claims (7)
1. a kind of compound micro-ecological preparation formula fermented available for field, it is characterised in that：The formula is by bacillus subtilis
The cfu/g of 5-15 hundred million, the cfu/g of lactic acid enterococcus faecalis 20-50 hundred million, Dextrose Monohydrate 100-200g/Kg, system chaff 350-500g/Kg and
Zeolite powder 250-350g/Kg is constituted.
2. the compound micro-ecological preparation formula according to claim 1 fermented available for field, it is characterised in that：Described
Lactic acid enterococcus faecalis is screened by screening washer, and the strain of 1 month can be no less than in 20 DEG C of the existence of preservation at room temperature by choosing.
3. the compound micro-ecological preparation formula according to claim 1 fermented available for field, it is characterised in that：Described
The preparation method of lactic acid enterococcus faecalis is as follows, chooses one plant of lactic acid enterococcus faecalis of screening in the cultured prawn enteron aisle of South Sea waters, will
One plant of lactic acid enterococcus faecalis first carries out that processing is dried again after freezing processing, freezing processing, it is made bacterium powder shape, and will
Bacterium powder is done material using phosphatide and is coated with, and the bacterium powder for being coated with completion is kept for 2 years under conditions of 20 DEG C, and in preservation
During carry out sampling observation month by month, the survival rate of monitoring lactic acid enterococcus faecalis in real time.
4. a kind of preparation method of the compound micro-ecological preparation formula fermented available for field, it is characterised in that：This method passes through
Bacillus subtilis, lactic acid enterococcus faecalis and culture medium are compounded, bacillus subtilis provides relative anaerobism for lactic acid enterococcus faecalis
Environment, cellulose family and other macromolecular components in culture medium are utilized for lactic acid Enterococcus faecalis fermentation.
5. the preparation method of the compound micro-ecological preparation formula according to claim 4 fermented available for field, its feature
It is：The formula of described culture medium is by yeast hydrolyate 100-200g/Kg, Dextrose Monohydrate 200-300g/Kg, potassium fulvate
100-150g/Kg, system chaff 200-300g/Kg and zeolite powder 100-200g/Kg compositions.
6. a kind of verification method of the compound micro-ecological preparation formula fermented available for field, it is characterised in that：This method is used
Orthogonal test carries out screening verification to probiotics formula, and the factor 1 is bacillus subtilis bacteria concentration, and the factor 2 is lactic acid excrement intestines
Coccus concentration, the factor 3 is culture medium concentration, monitors the concentration of lactic acid enterococcus faecalis in liquid after fermentation, organic acid concentration, screening
Go out most suitable probiotics formula and culture medium prescription.
7. the verification method of the compound micro-ecological preparation formula according to claim 6 fermented available for field, its feature
It is：Described culture medium prescription is by yeast hydrolyate 100-200g/Kg, Dextrose Monohydrate 200-300g/Kg, potassium fulvate
100-150g/Kg, system chaff 200-300g/Kg and zeolite powder 100-200g/Kg compositions.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710591746.8A CN107164232A (en) | 2017-07-19 | 2017-07-19 | The compound micro-ecological preparation formula and preparation method fermented available for field |
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| CN201710591746.8A CN107164232A (en) | 2017-07-19 | 2017-07-19 | The compound micro-ecological preparation formula and preparation method fermented available for field |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108359619A (en) * | 2018-02-01 | 2018-08-03 | 暨南大学 | Compound micro-ecological preparation and field fermentation process |
| CN109179685A (en) * | 2018-09-14 | 2019-01-11 | 广东渔夫宝水产科技有限公司 | A kind of synbiotic and its application method for aquaculture pool head viable bacteria fermentation |
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| CN105985916A (en) * | 2015-01-28 | 2016-10-05 | 河南惠通天下动物药业有限公司 | Composite microorganism preparation and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108359619A (en) * | 2018-02-01 | 2018-08-03 | 暨南大学 | Compound micro-ecological preparation and field fermentation process |
| CN109179685A (en) * | 2018-09-14 | 2019-01-11 | 广东渔夫宝水产科技有限公司 | A kind of synbiotic and its application method for aquaculture pool head viable bacteria fermentation |
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Application publication date: 20170915 |