CN106519059A - Preparation method of enteromorpha prolifera polysaccharide - Google Patents
Preparation method of enteromorpha prolifera polysaccharide Download PDFInfo
- Publication number
- CN106519059A CN106519059A CN201611156763.0A CN201611156763A CN106519059A CN 106519059 A CN106519059 A CN 106519059A CN 201611156763 A CN201611156763 A CN 201611156763A CN 106519059 A CN106519059 A CN 106519059A
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- Prior art keywords
- preparation
- enteromorpha
- drying
- sea grass
- length
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
Abstract
The invention relates to a preparation method of enteromorpha prolifera polysaccharide. The preparation method of the enteromorpha prolifera polysaccharide comprises the following steps of (1) washing fresh enteromorpha with tap water until the electric conductivity is less than 20 us, and spin-drying through a dehydrator for standby use; (2) weighing 250g of dried enteromorpha, adding 10L of distilled water, soaking for 8h, and pounding 1mjn through a pounding machine at the speed of 16,000r/min; and (3) heating and stirring a product of the step (2) for 1 to 3h until the water temperature is ranged from 80 to 105 DEG C. The enteromorpha prolifera polysaccharide prepared by the method provided by the invention can be effectively used for reducing blood fat, reducing blood glucose, resisting tumors and the like, and is used as a marine organism medicine raw material so as to play roles in improving immunity and scavenging free radicals of a human body.
Description
Technical field
The present invention relates to a kind of preparation method of sea grass polysaccharide.
Background technology
The construction in Enteromorpha resource higher value application base will open height of the Enteromorpha resource in marine organisms pharmaceutical products field
End application.Enteromorpha is a kind of natural green-alga, and its nutritive value enriches very much, containing substantial amounts of Enteromorpha sulfated polysaccharide, green alga albumen,
Unrighted acid and natural mineral matter etc..Wherein sea grass polysaccharide is rhamnose-xylose-glucose aldehydic acid polymer.It is modern
Scientific investigations showed that, Enteromorpha sulfated polysaccharide isoreactivity composition has the biological function such as anti-oxidant, antitumor, reducing blood lipid.Enteromorpha
Polysaccharide is widely used, and effectively reducing blood lipid, hypoglycemic, antitumor etc. can act on as medicine, as marine organisms medical material
Can play enhance immunity very well, remove the ability of human free radical, add sea grass polysaccharide used in animal feed, can be with
Raising animal immunizing power is played, prevents the effects such as epizootic propagation.
The content of the invention
The present invention proposes a kind of preparation method of sea grass polysaccharide.
A kind of preparation method of sea grass polysaccharide, it is characterised in that comprise the following steps：
（1）Fresh Enteromorpha is taken, with being originally washed to electrical conductivity<20 us, dewaterer are standby after drying；
（2）Claim the drying each 250g of Enteromorpha, respectively plus distilled water lOL immersion 8h, lmjn is smashed to pieces with bruisher 16000r/min；
（3）Then by step（2）Product heats and stir, to water temperature 80-105 DEG C, when a length of 1-3h；
（4）Remaining part adds 95% ethanol to ethanol final concentration up to 80%, after standing 4h, under 10-30kg gravity at 2-8 DEG C from
Heart lOmin, separates supernatant precipitation, precipitation distillation water dissolves on a small quantity；
（5）Concentration is stirred at 70 DEG C with 800 mesh silk cover filterings, filtrate after being cooled to room temperature, afterwards with forced air drying 48h at 60 DEG C
After obtain final product.
Preferably, the step（1）Middle dehydration temperaturre is 60 DEG C, a length of 24h during dehydration.
Preferably, the step（3）Product heats and stir, to 95 DEG C of water temperature, when a length of 2h.
Preferably, the step（4）Centrifuging temperature is 4 DEG C, and gravity environment is 20kg.
Sea grass polysaccharide prepared by the method for the invention effectively can be acted in reducing blood lipid, hypoglycemic, antitumor etc., used as ocean
Biological medicine raw material can play enhance immunity very well, remove the ability of human free radical.
Specific embodiment
Embodiment 1.
A kind of preparation method of sea grass polysaccharide, comprises the following steps：
（1）Fresh Enteromorpha is taken, with being originally washed to electrical conductivity<20 us, dewaterer are standby after drying；
（2）Claim the drying each 250g of Enteromorpha, respectively plus distilled water lOL immersion 8h, lmjn is smashed to pieces with bruisher 16000r/min；
（3）Then by step（2）Product heats and stir, to water temperature 80-105 DEG C, when a length of 1-3h；
（4）Remaining part adds 95% ethanol to ethanol final concentration up to 80%, after standing 4h, under 10-30kg gravity at 2-8 DEG C from
Heart lOmin, separates supernatant precipitation, precipitation distillation water dissolves on a small quantity；
（5）Concentration is stirred at 70 DEG C with 800 mesh silk cover filterings, filtrate after being cooled to room temperature, afterwards with forced air drying 48h at 60 DEG C
After obtain final product.
Preferably, the step（1）Middle dehydration temperaturre is 60 DEG C, a length of 24h during dehydration.
Embodiment 2.
A kind of preparation method of sea grass polysaccharide, comprises the following steps：
（1）Fresh Enteromorpha is taken, with being originally washed to electrical conductivity<20 us, dewaterer are standby after drying；
（2）Claim the drying each 250g of Enteromorpha, respectively plus distilled water lOL immersion 8h, lmjn is smashed to pieces with bruisher 16000r/min；
（3）Then by step（2）Product heats and stir, to water temperature 80-105 DEG C, when a length of 1-3h；
（4）Remaining part adds 95% ethanol to ethanol final concentration up to 80%, after standing 4h, under 10-30kg gravity at 2-8 DEG C from
Heart lOmin, separates supernatant precipitation, precipitation distillation water dissolves on a small quantity；
（5）Concentration is stirred at 70 DEG C with 800 mesh silk cover filterings, filtrate after being cooled to room temperature, afterwards with forced air drying 48h at 60 DEG C
After obtain final product.
Preferably, the step（1）Middle dehydration temperaturre is 60 DEG C, a length of 24h during dehydration.
Preferably, the step（3）Product heats and stir, to 95 DEG C of water temperature, when a length of 2h.
Embodiment 3.
A kind of preparation method of sea grass polysaccharide, comprises the following steps：
（1）Fresh Enteromorpha is taken, with being originally washed to electrical conductivity<20 us, dewaterer are standby after drying；
（2）Claim the drying each 250g of Enteromorpha, respectively plus distilled water lOL immersion 8h, lmjn is smashed to pieces with bruisher 16000r/min；
（3）Then by step（2）Product heats and stir, to water temperature 80-105 DEG C, when a length of 1-3h；
（4）Remaining part adds 95% ethanol to ethanol final concentration up to 80%, after standing 4h, under 10-30kg gravity at 2-8 DEG C from
Heart lOmin, separates supernatant precipitation, precipitation distillation water dissolves on a small quantity；
（5）Concentration is stirred at 70 DEG C with 800 mesh silk cover filterings, filtrate after being cooled to room temperature, afterwards with forced air drying 48h at 60 DEG C
After obtain final product.
Preferably, the step（1）Middle dehydration temperaturre is 60 DEG C, a length of 24h during dehydration.
Preferably, the step（3）Product heats and stir, to 95 DEG C of water temperature, when a length of 2h.
Preferably, the step（4）Centrifuging temperature is 4 DEG C, and gravity environment is 20kg.
Embodiment 4.
A kind of preparation method of sea grass polysaccharide, comprises the following steps：
（1）Fresh Enteromorpha is taken, with being originally washed to electrical conductivity<20 us, dewaterer are standby after drying；
（2）Claim the drying each 250g of Enteromorpha, respectively plus distilled water lOL immersion 8h, lmjn is smashed to pieces with bruisher 16000r/min；
（3）Then by step（2）Product heats and stir, to water temperature 80-105 DEG C, when a length of 1-3h；
（4）Remaining part adds 95% ethanol to ethanol final concentration up to 80%, after standing 4h, under 10-30kg gravity at 2-8 DEG C from
Heart lOmin, separates supernatant precipitation, precipitation distillation water dissolves on a small quantity；
（5）Concentration is stirred at 70 DEG C with 800 mesh silk cover filterings, filtrate after being cooled to room temperature, afterwards with forced air drying 48h at 60 DEG C
After obtain final product；
（6）The step（1）Middle dehydration temperaturre is 60 DEG C, a length of 24h during dehydration；
（7）The step（3）Product heats and stir, to 95 DEG C of water temperature, when a length of 2h；
（8）The step（4）Centrifuging temperature is 4 DEG C, and gravity environment is 20kg.
Claims (4)
1. a kind of preparation method of sea grass polysaccharide, it is characterised in that comprise the following steps：
（1）Fresh Enteromorpha is taken, with being originally washed to electrical conductivity<20 us, dewaterer are standby after drying；
（2）Claim the drying each 250g of Enteromorpha, respectively plus distilled water lOL immersion 8h, lmjn is smashed to pieces with bruisher 16000r/min；
（3）Then by step（2）Product heats and stir, to water temperature 80-105 DEG C, when a length of 1-3h；
（4）Remaining part adds 95% ethanol to ethanol final concentration up to 80%, after standing 4h, under 10-30kg gravity at 2-8 DEG C from
Heart 10min, separates supernatant precipitation, precipitation distillation water dissolves on a small quantity；
（5）Concentration is stirred at 70 DEG C with 800 mesh silk cover filterings, filtrate after being cooled to room temperature, afterwards with forced air drying 48h at 60 DEG C
After obtain final product.
2. a kind of as claimed in claim 1 preparation method of sea grass polysaccharide, it is characterised in that the step（1）Middle dehydration temperaturre is
60 DEG C, a length of 24h during dehydration.
3. a kind of as claimed in claim 1 preparation method of sea grass polysaccharide, it is characterised in that the step（3）Product heats simultaneously
Stirring, to 95 DEG C of water temperature, when a length of 2h.
4. a kind of as claimed in claim 1 preparation method of sea grass polysaccharide, it is characterised in that the step（4）Centrifuging temperature is 4
DEG C, gravity environment is 20kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611156763.0A CN106519059A (en) | 2016-12-15 | 2016-12-15 | Preparation method of enteromorpha prolifera polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611156763.0A CN106519059A (en) | 2016-12-15 | 2016-12-15 | Preparation method of enteromorpha prolifera polysaccharide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106519059A true CN106519059A (en) | 2017-03-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611156763.0A Pending CN106519059A (en) | 2016-12-15 | 2016-12-15 | Preparation method of enteromorpha prolifera polysaccharide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107011456A (en) * | 2017-04-28 | 2017-08-04 | 中国海洋大学 | A kind of green algae polysaccharide and preparation method thereof |
| CN107903334A (en) * | 2017-12-29 | 2018-04-13 | 福建海兴保健食品有限公司 | A kind of technique that green alga proteoglycan is extracted from fresh green alga |
| CN110755446A (en) * | 2019-10-11 | 2020-02-07 | 青岛大学附属医院 | Application of sulfated glucuronic acid-xylo-rhamnosan in enhancing immune function |
-
2016
- 2016-12-15 CN CN201611156763.0A patent/CN106519059A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107011456A (en) * | 2017-04-28 | 2017-08-04 | 中国海洋大学 | A kind of green algae polysaccharide and preparation method thereof |
| CN107903334A (en) * | 2017-12-29 | 2018-04-13 | 福建海兴保健食品有限公司 | A kind of technique that green alga proteoglycan is extracted from fresh green alga |
| CN110755446A (en) * | 2019-10-11 | 2020-02-07 | 青岛大学附属医院 | Application of sulfated glucuronic acid-xylo-rhamnosan in enhancing immune function |
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Application publication date: 20170322 |