CN106518884B - A kind of method that chlorophyll is extracted from safflower seedling - Google Patents
A kind of method that chlorophyll is extracted from safflower seedling Download PDFInfo
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- CN106518884B CN106518884B CN201610918408.6A CN201610918408A CN106518884B CN 106518884 B CN106518884 B CN 106518884B CN 201610918408 A CN201610918408 A CN 201610918408A CN 106518884 B CN106518884 B CN 106518884B
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- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains four or more hetero rings
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Abstract
The invention discloses it is a kind of from safflower seedling extract chlorophyll method, including：Root removal operation is carried out to fresh safflower seedling, in the protection atmosphere of inert gas, the fresh safflower seedling for removing root is squeezed using vacuum suction method; obtain juice and residue; the juice is filtered, the filter residue being obtained by filtration and residue are merged, obtains material to be extracted；The material to be extracted is extracted using the extracting method of immersion and microwave cooperating ultrasonic wave, obtains extracting solution；The extracting solution is filtered, and filtered fluid is adsorbed using macroreticular resin, collects the eluent rich in chlorophyll；The eluent for being rich in chlorophyll is centrifuged, detaches concentration and three-level reverse osmosis concentration, medicinal extract is obtained, and the medicinal extract is dried using desivac, obtains chlorophyll powder.The method provided by the invention that chlorophyll is extracted from safflower seedling has the advantages that high income, at low cost, low energy consumption, purity is high, coloury.
Description
Technical field
The present invention relates to chlorophyll extractive technique field, it is more particularly related to which a kind of extract from safflower seedling
The method of chlorophyll.
Background technology
Safflower seedling be feverfew safflower tender leaf seedling, China northeast, North China, northwest and Shandong, Zhejiang, Guizhou, Sichuan,
The ground such as Tibet are cultivated extensively, have the effect of subdhing swelling and detoxicating, spleen benefiting and stimulating the appetite, are usually used in applying swimming and be swollen.Containing abundant in safflower seedling
Chlorophyll.
Chlorophyll is that plant carries out photosynthetic primary pigments, is a kind of fat-containing pigment family, positioned at thylakoid membrane.
Chlorophyll is magnesium porphyrin compound, including chlorophyll a, b, c, d, f and protochlorophyll and bacteriochlorophyll etc..Chlorophyll is not bery
Stablize, light, acid, alkali, oxygen, oxidant etc. can all make its decomposition.Under acid condition, chlorophyll molecule is easy to lose in porphyrin ring
Magnesium become de-magnesium pheophytin.Chlorophyll has hematopoiesis, beauty treatment and antiviral etc. universally present in most of green plantss
Multiple efficacies, but for the method that the extraction of chlorophyll still uses acetone and ether mostly, both organic reagents have in itself
Very big toxicity, also can be with certain injury to human body, a large amount of uses of this toxic reagent, meeting in industrialized production
Very big pollution is brought to environment.
Invention content
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of methods that chlorophyll is extracted from safflower seedling, are to remove root
The fresh safflower seedling in portion is raw material, and fresh safflower seedling is squeezed, with the slag charge obtained to squeezing impregnated with
And the mode that microwave and ultrasound combines extracts slag charge, and passes through filtering, resin adsorption and concentration, prepares
The chlorophyll that high-purity, high-content, high yield, color and luster be good, property is stablized is obtained, while the juice that expressing process obtains may be used also
For other health products, drink or various aspects, so as to carry out rational utilization to the nutritional ingredient in safflower seedling, improve
The use value of safflower seedling.
To achieve these goals, the present invention provides a kind of method that chlorophyll is extracted from safflower seedling, including following
Step：
Step 1: root removal operation is carried out to fresh safflower seedling, in the protection atmosphere of inert gas, using vacuum
Negative pressure method squeezes the fresh safflower seedling for removing root, obtains juice and residue, uses 0.1~0.2 μm of filter membrane
The juice is filtered, the filter residue being obtained by filtration and residue are merged, obtains material to be extracted；
Step 2: being extracted using the extracting method of immersion and microwave cooperating ultrasonic wave to the material to be extracted, obtain
To extracting solution；
Step 3: the extracting solution is filtered using microfiltration membranes and ultrafiltration membrane successively, and using macroreticular resin to mistake
Filtrate is adsorbed, and reuses ethyl alcohol and is eluted, and collects the eluent rich in chlorophyll；
Step 4: being centrifuged to the eluent for being rich in chlorophyll, detaching concentration and three-level reverse osmosis concentration, soaked
Cream, and the medicinal extract is dried using desivac, obtain chlorophyll powder.
It is of the present invention that chlorophyll is extracted from safflower seedling, it squeezes the juice first to safflower seedling, to the juice squeezed the juice
Liquid carries out micro-filtration and ultrafiltration obtains filter residue, the residue that filter residue and squeezing obtain is merged to obtain material to be extracted, due to squeezing the juice
Obtained juice is limpid, therefore convenient for filtering, this shortens extraction process and reduces extraction difficulty to a certain extent, together
When juice-extracting process also safflower seedling is crushed, lay a good groundwork for subsequent extraction process, this also contracts to a certain extent
The short extraction time of chlorophyll, and improve extract yield；
The present invention squeezes the juice to safflower seedling, can obtain the juice of slag charge and clear, contains in slag charge abundant
Chlorophyll extracts slag charge the chlorophyll with regard to that can obtain high-content；Also contain on a small quantity in the juice of clear simultaneously
Chlorophyll dusts to juice, can obtain the powder containing a small amount of chlorophyll, the powder can also be used for cosmetics, health products,
Drink, high-grade pharmaceuticals etc., so as to substantially increase the use value of safflower seedling and use scope；In the prior art, with red
It before flower seedling extracts chlorophyll for raw material, needs to dry safflower seedling, dry or fried dry processing, the process of drying can cause
A large amount of losses of safflower seedling Determination of Chlorophyll easily generate the carcinogenic substances such as BaP, drying and fried dry mistake during drying and fried dry
Journey will also result in a large amount of losses of nutritional ingredient, and can influence the color and luster of chlorophyll, and fried dry process can also cause to be charred taste, shadow
Ring the mouthfeel of chlorophyll.And it is of the invention using fresh safflower seedling as raw material, and the method squeezed the juice is used in combination, safflower can be lockked
Whole moisture in seedling, avoid during moisture evaporation, and moisture carries part nutriment and scatters and disappears together, so as to ensure
The content of chlorophyll in safflower seedling can obtain by subsequent extracting method the chlorophyll of high-content；
The present invention selects spiral juice extractor that can fully be squeezed the juice to fresh safflower seedling, suitable for industrialized production, and
And the process squeezed the juice does not damage the fibr tissue of safflower seedling, therefore convenient for subsequent filtering, will not cause the stifled of filter process
Plug, solves and fresh raw material is beaten in the prior art, when denser slurries are filtered, can result in blockage
Problem.And it is squeezed under the protection atmosphere of inert gas, so as to avoid in expressing process, the leaf in safflower seedling is green
There is the phenomenon that being aoxidized in element.The pretreatment mode squeezed to fresh raw material of present invention protection, and select effect compared with
Good spiral juice extractor, also with other kinds of juice extractor is used, such as air bag juice extractor, this can according to practical condition into
Row selection.
And it can reduce cost using the extracting method of immersion and microwave cooperating ultrasonic wave and shorten extraction time
On the basis of, improve recovery rate；And eluent can be concentrated using separation concentration and three-level reverse osmosis concentration, so as to keep away
Exempted from using boiling concentration process and caused by the loss of chlorophyll and the consuming of the energy；And using desivac to concentration
Medicinal extract afterwards is dried, and avoids and is dusted to medicinal extract in the prior art and prepare powder, 200 degrees Celsius or more of high temperature
The problem of high temperature is charred and lumps is be easy to cause, The present invention reduces process loss, improve the recovery rate of chlorophyll.
Preferably, it is described from safflower seedling extract chlorophyll method in, in the step 2, to material to be extracted into
Row extraction process be specially：
It impregnates：Material to be extracted is added in the ethyl alcohol that concentration of volume percent is 75% and impregnates 3~7min, material to be extracted
The mass ratio for the ethyl alcohol for being 75% with concentration of volume percent is 1:1.5~2.5；
Microwave treatment：Material to be extracted after immersion is subjected to microwave together with the ethyl alcohol that concentration of volume percent is 75%
Processing, microwave power are 100~150W, and the microwave time is 1~1.5min；
Ultrasonication：A certain amount of concentration of volume percent is added in the solidliquid mixture obtained after microwave treatment is
75% ethyl alcohol, and ultrasonication is carried out, ultrasonic power is 50~100W, and ultrasonic time is 1~3min, so as to be carried
Take liquid.
The present invention not only shortens extraction time, more in a manner that immersion, microwave treatment and ultrasonication are combined
Substantially increase the recovery rate of chlorophyll.
Preferably, in the method that chlorophyll is extracted from safflower seedling, in the step 3, the suction to filtered fluid
Echoing elution process is：
Adsorption process：Absorption flow is 1BV/h, and adsorption time is 1.0 hours；
Elution process：Be eluted to using deionized water it is colourless, using concentration of volume percent be 15% ethyl alcohol carry out
Elution, elution flow are 2BV/h, and elution time is 0.5 hour.
Preferably, in the method that chlorophyll is extracted from safflower seedling, in the step 3, successively using 0.1
~0.5 μm of microfiltration membranes and 0.005~0.01 μm of ultrafiltration membrane are filtered the extracting solution.
Preferably, in the method that chlorophyll is extracted from safflower seedling, the eluent that will be enriched in chlorophyll exists
5~8min is centrifuged under the rotating speed of 3000~3500r/min, separation concentration then is carried out to the supernatant after centrifugation.
Preferably, in the method that chlorophyll is extracted from safflower seedling, in the step 4, after being concentrated to separation
Separation concentrated solution carry out a three-level reverse osmosis concentration and secondary three reverse osmosis concentrations successively；
Three-level reverse osmosis concentration：The separation concentrated solution is obtained into level-one after the concentration of first-stage reverse osmosis component
Concentrate and level-one transparent liquid, the level-one transparent liquid enter two-pass reverse osmosis component and are concentrated to give secondary concentration liquid and two level
Transparent liquid, the two level transparent liquid enter three-level reverse osmosis module and are concentrated to give three-level concentrate and three-level transparent liquid；
Secondary three-level reverse osmosis concentration：By the primary concentration liquid, the secondary concentration liquid and the three-level concentrate into
Row mixing, and mixed mixed liquor is subjected to three-level reverse osmosis concentration again according to the step of three-level reverse osmosis concentration,
So as to obtain medicinal extract.The present invention can obtain the good medicinal extract of concentrated effect, and subtract by detaching concentration and three-level reverse osmosis concentration
The problem of having lacked energy consumption, having solved in the prior art by being concentrated by evaporation, and cause energy waste.
Preferably, in the method that chlorophyll is extracted from safflower seedling, the vacuum suction method is to removing root
Fresh safflower seedling squeezed, juice and residue are obtained, including being divided into two steps of single pressing and twice pressing；
Wherein, the single pressing is the 10~15min that squeezes the juice under the negative pressure of 0.4~0.6 atmospheric pressure；
The twice pressing is after the safflower seedling after single pressing is rolled, and squeezes the juice under 0.8~1 atmospheric pressure
1~5min obtains residue, and collects the juice of single pressing and the juice of twice pressing, and it is filtered, and will filter
To filter residue merged with residue, obtain material to be extracted.
Preferably, in the method that chlorophyll is extracted from safflower seedling, soaking process is to add in material to be extracted
5min is impregnated into the ethyl alcohol that concentration of volume percent is 75%, material to be extracted is 75% ethyl alcohol with concentration of volume percent
Mass ratio is 1:2.
Preferably, in the method that chlorophyll is extracted from safflower seedling, microwave process is to wait to carry after immersion
Feeding carries out microwave treatment, microwave power 128W together with the ethyl alcohol that concentration of volume percent is 75%, and the microwave time is
1.2min。
The present invention includes at least following advantageous effect：
1st, it is of the present invention from safflower seedling extract chlorophyll method be using remove the fresh safflower seedling of root as
Raw material, and fresh safflower seedling is squeezed, it is impregnated with the slag charge obtained to squeezing and microwave and ultrasound combines
Mode slag charge is extracted, and pass through filtering, resin adsorption and concentration, be prepared high-purity, Gao Han
The chlorophyll that amount, high yield, color and luster are good, property is stablized, while the juice that expressing process obtains can be additionally used in other health products, drink
Product or various aspects so as to carry out rational utilization to the nutritional ingredient in safflower seedling, improve the use value of safflower seedling.
2nd, the method for the present invention that chlorophyll is extracted from safflower seedling is simple for process, and reaction condition is mild and energy
It enough reduces the dosage of alcohol solvent and improves the rate of recovery of alcohol solvent, less energy consumption and water consume, greatly reduce production
Cost.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Description of the drawings
Fig. 1 is the flow chart of the method that chlorophyll is extracted from safflower seedling described in one embodiment of the invention.
Specific embodiment
Below in conjunction with the accompanying drawings and embodiment the present invention is described in further detail, those skilled in the art to be enabled to join
Book word can be implemented according to this as directed.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded one or more
The presence or addition of a other element or combinations.
The present invention provides a kind of method that chlorophyll is extracted from safflower seedling, specific flow of extracting refers to Fig. 1, in order to
The extraction process that the present invention explains safflower seedling Determination of Chlorophyll is better described, it will be carried out in detail with specific embodiment below
Elaboration.
Embodiment 1
First, root removal operation is carried out to fresh safflower seedling, in the protection atmosphere of nitrogen, using vacuum suction method pair
It is the negative pressure in 0.4 atmospheric pressure that 2kg, which removes the fresh safflower seedling progress single pressing of root and twice pressing, single pressing,
Under squeeze the juice 15min, twice pressing is after the safflower seedling after single pressing is rolled, and squeezes the juice under 0.8 atmospheric pressure
5min obtains residue, and collects the juice of single pressing and the juice of twice pressing, using 0.1 μm of filter membrane to juice into
Row filtering, the filter residue being obtained by filtration with residue is merged, obtains material to be extracted.
2nd, material to be extracted is added in the ethyl alcohol that concentration of volume percent is 75% and impregnates 3min, material and body to be extracted
The mass ratio of ethyl alcohol that product percent concentration is 75% is 1:1.5；Material to be extracted after immersion is dense together with percent by volume
The ethyl alcohol spent for 75% carries out microwave treatment, microwave power 100W together, and the microwave time is 1.5min；After microwave treatment
To solidliquid mixture in add in the ethyl alcohol that a certain amount of concentration of volume percent is 75%, and carry out ultrasonication, ultrasound
Wave power is 50W, ultrasonic time 3min, so as to obtain extracting solution.
3rd, the extracting solution is filtered using 0.1 μm of microfiltration membranes and 0.01 μm of ultrafiltration membrane successively, and uses macropore tree
Fat adsorbs filtered fluid, reuses ethyl alcohol and is eluted, and collects the eluent rich in chlorophyll；
Adsorption process：Absorption flow is 1BV/h, and adsorption time is 1.0 hours；
Elution process：Be eluted to using deionized water it is colourless, using concentration of volume percent be 15% ethyl alcohol carry out
Elution, elution flow are 2BV/h, and elution time is 0.5 hour.
4th, the eluent that will be enriched in chlorophyll centrifuges 8min under the rotating speed of 3000r/min, then using permeable membrane to from
Supernatant after the heart carries out separation concentration；
To separation concentrate after separation concentrated solution carry out successively a three-level reverse osmosis concentration and it is secondary three it is reverse osmosis concentrated
Contracting；
Three-level reverse osmosis concentration：The separation concentrated solution is obtained into level-one after the concentration of first-stage reverse osmosis component
Concentrate and level-one transparent liquid, the level-one transparent liquid enter two-pass reverse osmosis component and are concentrated to give secondary concentration liquid and two level
Transparent liquid, the two level transparent liquid enter three-level reverse osmosis module and are concentrated to give three-level concentrate and three-level transparent liquid；
Secondary three-level reverse osmosis concentration：By the primary concentration liquid, the secondary concentration liquid and the three-level concentrate into
Row mixing, and mixed mixed liquor is subjected to three-level reverse osmosis concentration again according to the step of three-level reverse osmosis concentration,
So as to obtain medicinal extract.
5th, medicinal extract is lyophilized, it is 98% to obtain purity, recovery rate 83%, and quality is the chlorophyll powder of 1.1g.
Embodiment 2
First, root removal operation is carried out to fresh safflower seedling, in the protection atmosphere of nitrogen, using vacuum suction method pair
It is the negative pressure in 0.6 atmospheric pressure that 2kg, which removes the fresh safflower seedling progress single pressing of root and twice pressing, single pressing,
Under squeeze the juice 10min, twice pressing is after the safflower seedling after single pressing is rolled, and the 1min that squeezes the juice at 1 atmosphere pressure,
Residue is obtained, and collects the juice of single pressing and the juice of twice pressing, juice was carried out using 0.2 μm of filter membrane
Filter, the filter residue being obtained by filtration with residue is merged, obtains material to be extracted.
2nd, material to be extracted is added in the ethyl alcohol that concentration of volume percent is 75% and impregnates 7min, material and body to be extracted
The mass ratio of ethyl alcohol that product percent concentration is 75% is 1:2.5；Material to be extracted after immersion is dense together with percent by volume
The ethyl alcohol spent for 75% carries out microwave treatment, microwave power 150W together, and the microwave time is 1min；It is obtained after microwave treatment
Solidliquid mixture in add in the ethyl alcohol that a certain amount of concentration of volume percent is 75%, and carry out ultrasonication, ultrasonic wave
Power is 100W, ultrasonic time 1min, so as to obtain extracting solution.
3rd, the extracting solution is filtered using 0.5 μm of microfiltration membranes and 0.005 μm of ultrafiltration membrane successively, and uses macropore
Resin adsorbs filtered fluid, reuses ethyl alcohol and is eluted, and collects the eluent rich in chlorophyll；
Adsorption process：Absorption flow is 1BV/h, and adsorption time is 1.0 hours；
Elution process：Be eluted to using deionized water it is colourless, using concentration of volume percent be 15% ethyl alcohol carry out
Elution, elution flow are 2BV/h, and elution time is 0.5 hour.
4th, the eluent that will be enriched in chlorophyll centrifuges 5min under the rotating speed of 3500r/min, then using permeable membrane to from
Supernatant after the heart carries out separation concentration；
To separation concentrate after separation concentrated solution carry out successively a three-level reverse osmosis concentration and it is secondary three it is reverse osmosis concentrated
Contracting；
Three-level reverse osmosis concentration：The separation concentrated solution is obtained into level-one after the concentration of first-stage reverse osmosis component
Concentrate and level-one transparent liquid, the level-one transparent liquid enter two-pass reverse osmosis component and are concentrated to give secondary concentration liquid and two level
Transparent liquid, the two level transparent liquid enter three-level reverse osmosis module and are concentrated to give three-level concentrate and three-level transparent liquid；
Secondary three-level reverse osmosis concentration：By the primary concentration liquid, the secondary concentration liquid and the three-level concentrate into
Row mixing, and mixed mixed liquor is subjected to three-level reverse osmosis concentration again according to the step of three-level reverse osmosis concentration,
So as to obtain medicinal extract.
5th, medicinal extract is lyophilized, obtains the chlorophyll powder that purity is 98.5%, recovery rate 90%, quality are 2.6g
End.
Embodiment 3
First, root removal operation is carried out to fresh safflower seedling, in the protection atmosphere of nitrogen, using vacuum suction method pair
It is the negative pressure in 0.5 atmospheric pressure that 2kg, which removes the fresh safflower seedling progress single pressing of root and twice pressing, single pressing,
Under squeeze the juice 12min, twice pressing is after the safflower seedling after single pressing is rolled, and squeezes the juice under 0.9 atmospheric pressure
3min obtains residue, and collects the juice of single pressing and the juice of twice pressing, using 0.15 μm of filter membrane to juice into
Row filtering, the filter residue being obtained by filtration with residue is merged, obtains material to be extracted.
2nd, material to be extracted is added in the ethyl alcohol that concentration of volume percent is 75% and impregnates 5min, material and body to be extracted
The mass ratio of ethyl alcohol that product percent concentration is 75% is 1:2；By the material to be extracted after immersion together with concentration of volume percent
Ethyl alcohol for 75% carries out microwave treatment, microwave power 128W together, and the microwave time is 1.2min；It is obtained after microwave treatment
Solidliquid mixture in add in the ethyl alcohol that a certain amount of concentration of volume percent is 75%, and carry out ultrasonication, ultrasonic wave
Power is 75W, ultrasonic time 2min, so as to obtain extracting solution.
3rd, the extracting solution is filtered using 0.2 μm of microfiltration membranes and 0.003 μm of ultrafiltration membrane successively, and uses macropore
Resin adsorbs filtered fluid, reuses ethyl alcohol and is eluted, and collects the eluent rich in chlorophyll；
Adsorption process：Absorption flow is 1BV/h, and adsorption time is 1.0 hours；
Elution process：Be eluted to using deionized water it is colourless, using concentration of volume percent be 15% ethyl alcohol carry out
Elution, elution flow are 2BV/h, and elution time is 0.5 hour.
4th, the eluent that will be enriched in chlorophyll centrifuges 6min under the rotating speed of 3300r/min, then using permeable membrane to from
Supernatant after the heart carries out separation concentration；
To separation concentrate after separation concentrated solution carry out successively a three-level reverse osmosis concentration and it is secondary three it is reverse osmosis concentrated
Contracting；
Three-level reverse osmosis concentration：The separation concentrated solution is obtained into level-one after the concentration of first-stage reverse osmosis component
Concentrate and level-one transparent liquid, the level-one transparent liquid enter two-pass reverse osmosis component and are concentrated to give secondary concentration liquid and two level
Transparent liquid, the two level transparent liquid enter three-level reverse osmosis module and are concentrated to give three-level concentrate and three-level transparent liquid；
Secondary three-level reverse osmosis concentration：By the primary concentration liquid, the secondary concentration liquid and the three-level concentrate into
Row mixing, and mixed mixed liquor is subjected to three-level reverse osmosis concentration again according to the step of three-level reverse osmosis concentration,
So as to obtain medicinal extract.
5th, medicinal extract is lyophilized, obtains the chlorophyll powder that purity is 99.2%, recovery rate 92%, quality are 3.0g
End.
Although the embodiments of the present invention have been disclosed as above, but its be not restricted in specification and embodiment it is listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, it is of the invention and unlimited
In specific details and legend shown and described herein.
Claims (8)
- A kind of 1. method that chlorophyll is extracted from safflower seedling, which is characterized in that include the following steps：Step 1: root removal operation is carried out to fresh safflower seedling, in the protection atmosphere of inert gas, using negative pressure of vacuum Method squeezes the fresh safflower seedling for removing root, juice and residue is obtained, using 0.1~0.2 μm of filter membrane to institute It states juice to be filtered, the filter residue being obtained by filtration and residue is merged, obtain material to be extracted；Step 2: being extracted using the extracting method of immersion and microwave cooperating ultrasonic wave to the material to be extracted, carried Take liquid；Step 3: the extracting solution is filtered using microfiltration membranes and ultrafiltration membrane successively, and using macroreticular resin to filtered fluid It is adsorbed, reuses ethyl alcohol and eluted, collect the eluent rich in chlorophyll；Step 4: being centrifuged to the eluent for being rich in chlorophyll, detaching concentration and three-level reverse osmosis concentration, medicinal extract is obtained, And the medicinal extract is dried using desivac, obtain chlorophyll powder；In the step 2, the process extracted to material to be extracted is specially：It impregnates：Material to be extracted is added in the ethyl alcohol that concentration of volume percent is 75% and impregnates 3~7min, material and body to be extracted The mass ratio of ethyl alcohol that product percent concentration is 75% is 1:1.5~2.5；Microwave treatment：Material to be extracted after immersion is carried out together with the ethyl alcohol that concentration of volume percent is 75% at microwave Reason, microwave power are 100~150W, and the microwave time is 1~1.5min；Ultrasonication：It is 75% that a certain amount of concentration of volume percent is added in the solidliquid mixture obtained after microwave treatment Ethyl alcohol, and carry out ultrasonication, ultrasonic power is 50~100W, and ultrasonic time is 1~3min, so as to be extracted Liquid.
- 2. the method for chlorophyll is extracted from safflower seedling as described in claim 1, which is characterized in that right in the step 3 The absorption of filtered fluid and elution process are：Adsorption process：Absorption flow is 1BV/h, and adsorption time is 1.0 hours；Elution process：Be eluted to using deionized water it is colourless, using concentration of volume percent be 15% ethyl alcohol eluted, Elution flow is 2BV/h, and elution time is 0.5 hour.
- 3. the method for chlorophyll is extracted from safflower seedling as described in claim 1, which is characterized in that in the step 3, according to It is secondary that the extracting solution is filtered using 0.1~0.5 μm of microfiltration membranes and 0.005~0.01 μm of ultrafiltration membrane.
- 4. the method for chlorophyll is extracted from safflower seedling as described in claim 1, which is characterized in that will be enriched in washing for chlorophyll De- liquid centrifuges 5~8min under the rotating speed of 3000~3500r/min, then carries out separation concentration to the supernatant after centrifugation.
- 5. the method for chlorophyll is extracted from safflower seedling as described in claim 1, which is characterized in that right in the step 4 Separation concentrated solution after separation concentration carries out a three-level reverse osmosis concentration and secondary three reverse osmosis concentrations successively；Three-level reverse osmosis concentration：The separation concentrated solution is obtained into primary concentration after the concentration of first-stage reverse osmosis component Liquid and level-one transparent liquid, the level-one transparent liquid enter two-pass reverse osmosis component and are concentrated to give secondary concentration liquid and two level is transparent Liquid, the two level transparent liquid enter three-level reverse osmosis module and are concentrated to give three-level concentrate and three-level transparent liquid；Secondary three-level reverse osmosis concentration：The primary concentration liquid, the secondary concentration liquid and the three-level concentrate are mixed It closes, and mixed mixed liquor is subjected to three-level reverse osmosis concentration again according to the step of three-level reverse osmosis concentration, so as to Obtain medicinal extract.
- 6. the method for chlorophyll is extracted from safflower seedling as described in claim 1, which is characterized in that the vacuum suction method pair The fresh safflower seedling for removing root is squeezed, and juice and residue is obtained, including being divided into single pressing and twice pressing Two steps；Wherein, the single pressing is the 10~15min that squeezes the juice under the negative pressure of 0.4~0.6 atmospheric pressure；The twice pressing is after the safflower seedling after single pressing is rolled, and squeeze the juice under 0.8~1 atmospheric pressure 1~ 5min obtains residue, and collects the juice of single pressing and the juice of twice pressing, and it is filtered, and will be obtained by filtration Filter residue merged with residue, obtain material to be extracted.
- 7. the method for chlorophyll is extracted from safflower seedling as described in claim 1, which is characterized in that soaking process is will to wait to carry Feeding, which is added in the ethyl alcohol that concentration of volume percent is 75%, impregnates 5min, and material to be extracted is 75% with concentration of volume percent Ethyl alcohol mass ratio be 1:2.
- 8. the method for chlorophyll is extracted from safflower seedling as described in claim 1, which is characterized in that microwave process is will to impregnate Material to be extracted afterwards carries out microwave treatment together with the ethyl alcohol that concentration of volume percent is 75%, microwave power 128W, micro- The wave time is 1.2min.
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| CN110269245A (en) * | 2019-06-05 | 2019-09-24 | 南阳师范学院 | A kind of wholefood nutrition fortifier-chlorophyll powder and preparation method thereof |
| CN110483532B (en) * | 2019-09-05 | 2021-02-12 | 卓士鸿 | Method for extracting chlorophyll and aromatic oil from Chinese prickly ash leaves |
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| CN102659792A (en) * | 2012-05-05 | 2012-09-12 | 邯郸市中进天然色素有限公司 | Method for extracting chlorophyll from clovers |
| CN105837582A (en) * | 2016-03-25 | 2016-08-10 | 青海师范大学 | Method of extracting chlorophyll from leaves of green plants |
| CN106008443A (en) * | 2016-07-22 | 2016-10-12 | 河南科技大学 | Extraction method of purple corn anthocyanin |
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