CN106508654A - Suitable hydroponic device for root system culture of Agrobacterium rhizogenes transgenic plant - Google Patents
Suitable hydroponic device for root system culture of Agrobacterium rhizogenes transgenic plant Download PDFInfo
- Publication number
- CN106508654A CN106508654A CN201611199677.8A CN201611199677A CN106508654A CN 106508654 A CN106508654 A CN 106508654A CN 201611199677 A CN201611199677 A CN 201611199677A CN 106508654 A CN106508654 A CN 106508654A
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- plant
- root
- grip block
- liquid tank
- transgenic
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention relates to a special hydroponics suitable for root system culture of Agrobacterium rhizogenes and its application, the device comprising a tank, a plant holder, wherein the plant holder comprises a vertical plate support, a clamping plate, and a plate-mounted frame body provided with a height adjustment groove and a chute provided with a combined upper-layer holding plate, a chute sealing strip and a light-shielding plate, the upper deck being provided with a plant gripping hole, the lower holding plate and the shading plate which are provided with corresponding root and stem holes. The tank is a rectangular box with a net length corresponding to the plant holder. The net width is an integral multiple of the width of the plant holder. The box contains an independent root zone corresponding to the root hole separated by a divider. The device configuration also includes an oxygen pump, a root pruning bracket and a pouring funnel. The device can be used for root system culture of Agrobacterium rhizogenes transgenic plants.
Description
Technical field
The present invention is with regard to a kind of special hydroponic device suitable for Agrobacterium rhizogenes transgenic crosses plant root culture
And the method for carrying out transgenic root of hair water planting with the device.
Background technology
Agrobacterium rhizogenes after plant is infected can induce plant to produce root nodule, and further form a large amount of hyperbranched
Adventitious root, commonly referred to root of hair, with fast growth, the characteristics of differentiation degree is high.Root of hair can also in vitro
The complete plant of regeneration, now as whole strain plant contains transgene component, is completely transfer-gen plant；If root of hair infects
Plant stem or root simultaneously form root of hair, even if at this moment all wiping out the common roots of plant itself, root of hair also can replace well
Its function absorbs moisture and nutrient, and plant continues normally growth promoter.This incomplete transfer-gen plant is by containing transgenic
The root of composition and not genetically modified aerial partss are combined, i.e., so-called transgenic crosses plant.Transgenic crosses plant by
In the process for not needing root of hair regeneration plant, the time for obtaining transfer-gen plant is substantially reduced, simultaneously because its seed is not
Normal seed containing transgene component, transgene component will not entail the next generation, will not environmentally safe and bio-diversity
Impact, therefore be to carry out gene function, particularly in the material that the functional study of root expressing gene is ideal.This reality
Room is tested using Agrobacterium rhizogenes transgenic crosses plant technical system, a series of salt tolerants related Semen sojae atricolor ZFP, MYB is have studied,
HLH transcription factor genes related water channel protein gene TIP, ZIP drought-enduring to salt tolerant etc., it is obvious that successful identification has excavated a collection of energy
Improve the useful genetic resourceses of plant salt endurance.But in Agrobacterium rhizogenes transgenic crosses plant root culture link, also deposit
In trivial operations, inefficient problem.After preliminary acquisition Agrobacterium rhizogenes transgenic crosses plant, typically will per plant of plant
Strain is cultivated in being individually placed on teat glass and is taken root.This method operation is pretty troublesome and very wastes time and energy, it is difficult to which scale is fast
The carrying out of speed is cultivated.Its difficulty is mainly manifested in the following aspects：1）Non-transgenic Rhizoma Imperatae can only be taken when pruning one by one
Go out, be sequentially loaded into test tube after pruning well one by one again and fix；2）Test tube volume very little, needs frequently change nutritional solution daily, often
It is secondary to change single test tube.3）Must each test tube pump into air respectively, it is cumbersome. 4）It is not suitable for consolidating for tall and big plant
Fixed and culture.5）Teat glass is transparent, easily grows algae, affects experimental result.6）A large amount of test tubes and test tube rack is needed, is accounted for
It is big with space, not easy cleaning.The present invention is for the problems referred to above in Agrobacterium rhizogenes transgenic crosses plant root culture, pole
The earth simplifies operating process, improves efficiency, enables the root system culture of Agrobacterium rhizogenes transgenic crosses plant quick, high
Effect and the carrying out of scale.
The content of the invention
It is an object of the present invention to be directed to the problems referred to above that existing equipment and method are present, there is provided one kind is suitable to root of hair agriculture
The special hydroponic device of bacillus transgenic crosses plant root culture and its application.
The object of the present invention is achieved like this：It is a kind of to be suitable to the special of Agrobacterium rhizogenes transgenic crosses plant root culture
With hydroponic device, including reserving liquid tank, plant fixing frame, it is characterised in that：
1）Plant fixing frame includes that a pair of risers are supported, and two risers are supported by fixed lower floor's grip block and the vertical connection of level
Riser is solidified as a whole, and fixed lower floor's grip block supports wide with riser, is covered with dark slide, fixed lower floor on fixed lower floor grip block
Riser above grip block is supported at least provided with twice altitude mixture control draw-in groove arranged side by side, and close on altitude mixture control draw-in groove, edge is provided with one
The chute that road is communicated, is furnished with chute sealing strip in chute, be furnished with the clamping of combination type upper strata in altitude mixture control draw-in groove arranged side by side
Plate, combination type upper strata grip block are made up of the symmetrical monolithic of two panels, are provided with equally distributed plant folder in Liang Ge monolithics intersection
Hole is held, tenon on the outside of the end of two monolithics, is equipped with, tenon is slipped into and snapped in altitude mixture control draw-in groove downwards by chute, then is inserted
Enter chute sealing strip to position combination type upper strata grip block, after assembling, the width of combination type upper strata grip block is not more than riser
The width of support；
2）It is furnished with clamping sponge in the plant clamping hole of combination type upper strata grip block；Fixed lower floor's grip block and dark slide be provided with
The corresponding root hole in plant clamping hole and transforaminal in the grip block of combination type upper strata, wherein, root hole clamps hole more than plant, and transforaminal is equal to
The stalk diameter of plant；
3）Described reserving liquid tank is rectangular vessel, and in the case of reserving liquid tank, clear length is equal to the physical length of plant fixing frame, in case
Integral multiple of the span width for plant fixing frame width, reserving liquid tank bottom are provided with the leakage fluid dram containing tapping valve, and upper edge is provided with fluid injection and leads to
Road and inlet channel, are provided with the independent root system area separated with root system grille, described independent root system area in described reserving liquid tank
Correspond with plant clamping hole, transforaminal and root hole,
4）The setting height(from bottom) of fixed lower floor grip block is crossed less than the free height in reserving liquid tank, and higher than the height of grille.
In the present invention, reserving liquid tank at least configures the plant fixing frame with span width Corresponding matching in its case, is located at
The outside combination type upper strata grip block of the plant fixing frame at edge, fixed lower floor's grip block and dark slide be provided with one group it is corresponding
Mark buoy hole clipping and pump gas hole, and it is furnished with corresponding mark buoy and oxygen increasing pump.
In the present invention, described reservoir channel is furnished with filling funnel, the output pipe of oxygen increasing pump by inlet channel and
Pump gas hole enters reserving liquid tank.
In the present invention, the reserving liquid tank and dark slide are made by light tight material, and chute sealing strip is by gum elastic material
Material makes, and other of plant fixing frame are built by the making of water white transparency material.
In the present invention, a described special hydroponic device is also equipped with a root system pruning support, root system pruning support
By the gripper shoe groups of base plate and base plate both sides into by gripper shoe top braces plant fixing frame.
A kind of application of above-mentioned special hydroponic device, it is characterised in that:
A) empirically require to prepare plant culturing nutritional solution, reserving liquid tank is injected by reservoir channel；
B) the Jing Agrobacterium rhizogenes mediated transformations that are prepared in advance are taken, has tentatively had transgenic root of hair to produce in the nearly root position of plant
Transgenic crosses plant, only retain be probably transgenic root of hair root, other common roots all prune away, first by plant root
Lower floor's root hole of insertion plant fixing frame, installs dark slide, and plant stalk by passing in its transforaminal, adjusted with height by stalk middle and upper part
Parcel clamping sponge at section draw-in groove respective heights, clamps the plant clamping hole that sponge snaps in combination type upper strata grip block, upper strata folder
Hold the chute of plate insertion corresponding height and snapped in altitude mixture control draw-in groove downwards, combination type upper strata is clamped by chute sealing strip
Plate is spacing；C) plant fixing frame for fixing plant is close to into insertion reserving liquid tank side by side, dark slide should be less than reserving liquid tank side wall
Height, mark buoy and oxygen increasing pump are installed；D) water level mark buoy is observed, if liquid level is less than experimental standard, can be leaked with fluid injection
Bucket Jing liquid feedings duct supplements plant culturing nutritional solution；When requiring replacement, leakage fluid dram can be passed through by old plant culturing nutritional solution
Empty, then Jing reservoir channels add fresh plant culturing nutritional solution；E) Jing is wanted often to note checking whether after cultivating a couple of days
Plant root has new non-transgenic root to grow, and must wipe out if having, and method is integrally to take out plant fixing frame and lay
To on root system pruning bearing, the non-transgenic root of hair of inspection the plant root that carefully prunes away only retains transgenic root of hair；
F), after transgenic root of hair root growth and development is good, plant is integrally taken out carries out next step experiment.
The present invention technique effect be：As plant fixing frame has altitude mixture control draw-in groove, go for different plant heights
The fixation and culture of seedling.As plant fixing frame can be separated with reserving liquid tank, easily can integrally take out, be installed to root system
Prune support and batch pruning is carried out to transgenic root, these operations are all very convenient.As plant fixing frame is furnished with dark slide,
In reserving liquid tank can effectively being prevented, nutritional solution grows algae, it is ensured that its quality, while it is simultaneously real intuitively to observe liquid level
The quickly in good time supplement of existing nutritional solution, replacing.Present configuration is compact, easy to maintenance.
Description of the drawings
Fig. 1 is embodiments of the invention structural representation.
Fig. 2 is the structural representation of reserving liquid tank embodiment.
Fig. 3 is the riser supporting construction schematic diagram of plant fixing frame.
Fig. 4 is the standing part example structure schematic diagram of plant fixing frame.
Fig. 5 is anti-dazzle plate structure schematic diagram.
Fig. 6 is upper strata clamping plate structure schematic diagram.
Fig. 7 is plant fixing frame configuration example structure schematic diagram.
In figure：1st, reserving liquid tank, 2, plant fixing frame, 3, root system pruning support, 4, oxygen increasing pump, 5, filling funnel, 6, discharge opeing
Mouthful, 7, mark buoy, 8, grille, 9, reservoir channel, 10, inlet channel, 11, riser support, 12, altitude mixture control draw-in groove, 13,
Chute, 14, fixed lower floor's grip block, 15, root hole, 16, mark buoy hole clipping, 17, pump gas hole, 18, connection board, 19, shading
Plate, 20, transforaminal, 21, combination type upper strata grip block, 22, plant clamping hole, 23, tenon, 24, clamp sponge, 25, chute sealing
Bar, 26, jack.
Specific embodiment
The nonrestrictive concrete structure for disclosing the embodiment of the present invention of accompanying drawing, makees into one to the present invention below in conjunction with the accompanying drawings
The description of step.
From Fig. 1 and Fig. 2, the present invention includes reserving liquid tank 1, plant fixing frame 2, and wherein, described reserving liquid tank 1 is rectangle
Container, length of the length for plant fixing frame 2 in the case of reserving liquid tank 1, multiple of the span width for 2 width of plant fixing frame in case
Number, its bottom are provided with the leakage fluid dram 6 containing tapping valve, and edge is provided with reservoir channel 9 and inlet channel 10, described reserving liquid tank 1 thereon
The independent root system area of the separation of root system grille 8 is provided with inside；One special hydroponic device is also equipped with a root system pruning support 3, root
System prune support 3 by base plate and base plate both sides gripper shoe groups into.
From Fig. 3-Fig. 7, plant fixing frame 2 includes that a pair risers corresponding with length in reserving liquid tank 1 case support 11, two
Riser supports 11 to be solidified as a whole by level fixation lower floor grip block 14 and vertical connection board 18, on fixed lower floor grip block 14
Dark slide 19 is covered with, riser supports the top of 11 correspondence dark slides 19 at least provided with twice altitude mixture control draw-in groove 12 arranged side by side, high
Degree is adjusted and is connected with chute 13 above draw-in groove 12, is furnished with chute sealing strip 25, fixed lower floor grip block 14 and screening in chute 13
The height of tabula rasa 19 but must not exceed the free height in reserving liquid tank 1 case higher than grille.
A combination type upper strata grip block 21 is provided with altitude mixture control draw-in groove 12, and each combination type upper strata grip block 21 is by two
The symmetrical monolithic composition of individual half.Monolithic intersection is provided with string semicircular indentations, and the semicircular indentations of two monolithics are combined into one
Individual circular plant clamps hole 22, has tenon 23, can be slided into by chute 13 and snap in downwards altitude mixture control card on the outside of its two ends
Groove 12, is also made up of two half with each plant clamping 22 corresponding dark slide 19 of hole, also is provided with string where corresponding to thereon
Circular transforaminal 20, the aperture of root hole 15 clamp hole 22 more than plant, and the aperture of transforaminal 20 is the stalk diameter of plant.Plant clamps
Hole 22, transforaminal 20, root hole 15 and the root system area in reserving liquid tank 1 correspond, and root system of plant can be by fixed lower floor grip block
Root hole 15 and 19 transforaminal 20 of dark slide are placed in nutritional solution.One group of corresponding instruction can be provided with grip block 21 and dark slide 19
Buoy hole clipping 16 and pump gas hole 17.
In the present embodiment, it is rectangular foot that described riser supports 11 lower section, and is provided with four height tune arranged side by side
Section draw-in groove 12 and corresponding chute 13.In use, rectangular foot can be supported to straddle on the grille 8 at two ends by riser.
The spacing of the gripper shoe of the root system pruning support 3 of the present embodiment and width are corresponding with plant fixing frame 12, have and plant at the top of which
The corresponding jack 26 of rectangular foot in thing fixed mount 12, during pruning, the rectangular foot of plant fixing frame 2 may be inserted into support top of the plate
In the jack 26 in portion.It is furnished with clamping sponge 24 in described upper strata plant clamping hole 22；Described reservoir channel 9 is furnished with fluid injection leakage
Bucket 5, described inlet channel 10 is furnished with oxygen increasing pump 4.
When being embodied as, the reserving liquid tank 1, dark slide 19 are made by light tight material, and chute sealing strip 25 is by elastic rubber
Glue material makes.Other structures of plant fixing frame 2 are made by water white transparency material.
When the present invention is actually used, empirically requires first to prepare plant culturing nutritional solution, stored up by the injection of reservoir channel 9
Liquid case 1.Then the Jing Agrobacterium rhizogenes mediated transformations that are prepared in advance are taken, selects tentatively have transgenic to send out in the nearly root position of plant
The transgenic crosses plant that root is produced, only retains the root of possibly transgenic root of hair, and other common roots all prune away, and will first plant
Thing root inserts the root hole 15 of plant fixing frame 2, installs dark slide 19, plant stalk by passing in its transforaminal 22, in stalk on
Clamping sponge 24 is wrapped up at portion and 12 respective heights of altitude mixture control draw-in groove, the clamping hole that sponge 24 snaps in upper strata grip block is clamped
22, the chute 13 of upper strata grip block insertion corresponding height is simultaneously snapped in altitude mixture control draw-in groove 12 downwards, by chute sealing strip 25
It is spacing to grip block.The plant fixing frame 2 for fixing plant is close to into insertion reserving liquid tank 1 side by side, dark slide 19 should be less than storage
The height of 1 side wall of liquid case, installs mark buoy 7 and oxygen increasing pump 4.Jing often observes water level mark buoy 7, if liquid level is less than experiment mark
Standard, can supplement plant culturing nutritional solution with 5 Jing liquid feedings duct of filling funnel 9；When requiring replacement, can be by leakage fluid dram 6
Tapping valve old plant culturing nutritional solution is emptied, then Jing reservoir channels 9 add fresh plant culturing nutritional solution.Culture number
After it, Jing often attentions to check whether and have new non-transgenic Rhizoma Imperatae to grow in plant root, must wipe out if having, method is
Take out plant fixing frame 2 overall and be placed on root system pruning support 3, check and the non-of plant root that carefully prune away turns base
Because of root of hair, only retain transgenic root of hair.After transgenic root of hair root growth and development is good, plant is integrally taken out carries out next step reality
Test.
Claims (6)
1. a kind of special hydroponic device for being suitable to Agrobacterium rhizogenes transgenic crosses plant root culture, including reserving liquid tank, plant
Fixed mount, it is characterised in that：
1）Plant fixing frame includes that a pair of risers are supported, and two risers are supported by fixed lower floor's grip block and the vertical connection of level
Riser is solidified as a whole, and fixed lower floor's grip block supports wide with riser, is covered with dark slide, fixed lower floor on fixed lower floor grip block
Riser above grip block is supported at least provided with twice altitude mixture control draw-in groove arranged side by side, and close on altitude mixture control draw-in groove, edge is provided with one
The chute that road is communicated, is furnished with chute sealing strip in chute, be furnished with the clamping of combination type upper strata in altitude mixture control draw-in groove arranged side by side
Plate, combination type upper strata grip block are made up of the symmetrical monolithic of two panels, are provided with equally distributed plant folder in Liang Ge monolithics intersection
Hole is held, tenon on the outside of the end of two monolithics, is equipped with, tenon is slipped into and snapped in altitude mixture control draw-in groove downwards by chute, then is inserted
Enter chute sealing strip to position combination type upper strata grip block, after assembling, the width of combination type upper strata grip block is not more than riser
The width of support；
2）It is furnished with clamping sponge in the plant clamping hole of combination type upper strata grip block；Fixed lower floor's grip block and dark slide be provided with
The corresponding root hole in plant clamping hole and transforaminal in the grip block of combination type upper strata, wherein, root hole clamps hole more than plant, and transforaminal is equal to
The stalk diameter of plant；
3）Described reserving liquid tank is rectangular vessel, and in the case of reserving liquid tank, clear length is equal to the physical length of plant fixing frame, in case
Integral multiple of the span width for plant fixing frame width, reserving liquid tank bottom are provided with the leakage fluid dram containing tapping valve, and upper edge is provided with fluid injection and leads to
Road and inlet channel, are provided with the independent root system area separated with root system grille, described independent root system area in described reserving liquid tank
Correspond with plant clamping hole, transforaminal and root hole；
4）The setting height(from bottom) of fixed lower floor grip block is crossed less than the free height in reserving liquid tank, and higher than the height of grille.
2. the special hydroponic device for being suitable to Agrobacterium rhizogenes transgenic crosses plant root culture according to claim 1,
Characterized in that, reserving liquid tank at least configures the plant fixing frame with span width Corresponding matching in its case, positioned at the plant at edge
The outside combination type upper strata grip block of thing fixed mount, fixed lower floor's grip block and dark slide are provided with one group of corresponding mark buoy
Hole clipping and pump gas hole, and it is furnished with corresponding mark buoy and oxygen increasing pump.
3. the special hydroponic device for being suitable to Agrobacterium rhizogenes transgenic crosses plant root culture according to claim 2,
Characterized in that, described reservoir channel is furnished with filling funnel, the output pipe of oxygen increasing pump is entered by inlet channel and pump gas hole
Enter reserving liquid tank.
4. the special hydroponic device for being suitable to Agrobacterium rhizogenes transgenic crosses plant root culture according to claim 1,
It is characterized in that：The reserving liquid tank and dark slide are made by light tight material, and chute sealing strip is made by elastic rubber material, are planted
Other of thing fixed mount are built by the making of water white transparency material.
5. the special of Agrobacterium rhizogenes transgenic crosses plant root culture is suitable to according to claim 1 or 2 or 3 goods 4
Hydroponic device, it is characterised in that a described special hydroponic device is also equipped with a root system pruning support, root system pruning support
By the gripper shoe groups of base plate and base plate both sides into by gripper shoe top braces plant fixing frame.
6. the application of special hydroponic device described in a kind of claim 1, it is characterised in that:
A) empirically require to prepare plant culturing nutritional solution, reserving liquid tank is injected by reservoir channel；
B) the Jing Agrobacterium rhizogenes mediated transformations that are prepared in advance are taken, has tentatively had transgenic root of hair to produce in the nearly root position of plant
Transgenic crosses plant, only retain be probably transgenic root of hair root, other common roots all prune away, first by plant root
Lower floor's root hole of insertion plant fixing frame, installs dark slide, and plant stalk by passing in its transforaminal, adjusted with height by stalk middle and upper part
Parcel clamping sponge at section draw-in groove respective heights, clamps the plant clamping hole that sponge snaps in combination type upper strata grip block, upper strata folder
Hold the chute of plate insertion corresponding height and snapped in altitude mixture control draw-in groove downwards, combination type upper strata is clamped by chute sealing strip
Plate is spacing；
C) plant fixing frame for fixing plant is close to into insertion reserving liquid tank side by side, dark slide should be less than the height of reserving liquid tank side wall
Degree, installs mark buoy and oxygen increasing pump；
D) water level mark buoy is observed, if liquid level is less than experimental standard, plant culturing can be supplemented with filling funnel Jing liquid feedings duct
Nutritional solution；When requiring replacement, old plant culturing nutritional solution can be emptied by leakage fluid dram, then Jing reservoir channels add new
Fresh plant culturing nutritional solution；
E) wanting Jing often attentions to check whether after cultivating a couple of days have new non-transgenic root to grow in plant root, must cut if having
Remove, method is integrally taken out plant fixing frame and is placed on root system pruning bearing, check and the plant root that carefully prunes away
Non-transgenic root of hair, only retain transgenic root of hair；
F), after transgenic root of hair root growth and development is good, plant is integrally taken out carries out next step experiment.
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| CN201611199677.8A CN106508654B (en) | 2016-12-22 | 2016-12-22 | Special hydroponic device suitable for root system culture of agrobacterium rhizogenes transgenic combined plants |
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|---|---|---|---|
| CN201611199677.8A CN106508654B (en) | 2016-12-22 | 2016-12-22 | Special hydroponic device suitable for root system culture of agrobacterium rhizogenes transgenic combined plants |
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