CN106508419A - Wild efficient expanding propagation method for arbuscular mycorrhizal fungi - Google Patents
Wild efficient expanding propagation method for arbuscular mycorrhizal fungi Download PDFInfo
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- CN106508419A CN106508419A CN201510577555.7A CN201510577555A CN106508419A CN 106508419 A CN106508419 A CN 106508419A CN 201510577555 A CN201510577555 A CN 201510577555A CN 106508419 A CN106508419 A CN 106508419A
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Abstract
Arbuscular mycorrhiza is a mutual symbiont built by a kind of fungi in soil and a host plant root system. Research shows that AM can participate in many physiological and biochemical metabolism processes of plants and have multi-aspect beneficial effects on the plants. Existing AM fungus expanding propagation production has the defects that a soil matrix culture system is mainly adopted, sterilization treatment and high inoculation agent weight are needed, and transportation is inconvenient, so that application and popularization of the arbuscular mycorrhizae are restricted. Therefore, construction of a wild efficient expanding propagation method for the arbuscular mycorrhizae has the important significance. The invention relates to a wild efficient expanding propagation method for arbuscular mycorrhizal fungi. The method comprises the steps that field soil is disinfected with dazomet, corn and sorghum are adopted as host plants, and a soil sample is taken to be detected after cultivation is conducted for 3-6 months under normal management, wherein the density of spores in the soil is higher than 200/10 g, and the hyphal length is larger than 150 m/10 g.
Description
Technical field
The invention belongs to bacteria agent production technical field, more particularly to a kind of method of AMF field high efficiency expanding propagation.
Background technology
Arbuscular mycorrhiza (arbuscular mycorrhiza, abbreviation AM) is the mutualism that the class funguses in soil are set up with host plant root system
Body, belongs to endo-mycorrhiza.Most phanerogams are all mycorrhizal plantses in the world, wherein arbuscular mycorrhiza (arbuscular mycorrhizas, AM)
Account for 90%.At present, AM is the Hot Contents of Tiny ecosystem area research, and numerous studies show many Physiological and Biochemical Metabolism of AM mycorhiza energy involved in plant
Process, has many-sided effect, in most cases shows as to plant：Enter plant establishment, improve transplanting survival rate；Root system of plant is promoted to seek mineral nitrogen
Support and absorb, particularly improve absorption of the plant to phosphorus, N elements and trace element, improve phytomass；Stress resistance of plant is improved, mitigates soil-borne disease
Harmful generation, and the continuous cropping obstacle of crop is overcome to a certain extent；Improve crop quality, such as Nicotiana tabacum L., crops etc..
Up to the present, the pure culture of these funguses is not yet successful, it is impossible to as other can pass through industrial fermentation the microorganism of pure culture, come
The large-scale industrial production of AM fungal inoculum agent is carried out, thus also limits the large-area applications of AM funguses.Existing AM fungal inoculum agent life
On the basis of product technology is all built upon host and AM mycosymbiosises, current AM fungal inoculum agent production systems are divided into three major types：Soil matrix is trained
Foster system, non-soil-medium culture systems, without matrix culture system.Soil matrix culture systems be it is conventional, most reliable, thus be also application
A kind of Inoculant production system widest in area, but there is matrix components complexity, need sterilization treatment and Inoculant weight big, not readily transportable lacks
Point, constrains arbuscular mycorrhiza ground popularization and application.Therefore, set up a kind of arbuscular mycorrhiza field high efficiency expanding propagation method to have great importance, but at present
Related research is less.
The content of the invention
Present invention aim at：A kind of method of AMF field high efficiency expanding propagation is provided, and applies this method to arbuscular mycorrhiza field expanding propagation
Using in.
A kind of method of AMF field high efficiency expanding propagation, comprises the steps：
(1) first all soil surfaces are removed totally with the root of crop, stem, leaf etc. in soil is buried in；
(2) granule " will be mopped up greatly " to spread fertilizer over the fields in soil surface to be sterilized；
(3) medicament is ploughed deeply to the 20～30cm of depth of soil of sterilization, smashes big soil block, be the diffusion and infiltration of " mopping up greatly " decomposition gas
One good condition is provided；
(4) water to saturating, be close to soil surface and cover mulch film, be compacted around thin film, it is impossible to be ventilative；
(5), after fumigating 7～10 days, it is ventilated 10～15 days to open thin film, will loosen the soil daily 1～2 time during ventilation；
(6) after ventilated 10～15 days, arbuscular mycorrhiza microbial inoculum is sprinkled in soil surface, sprinkle Semen Maydiss again or the seed of Sorghum vulgare Pers. (is broadcast on microbial inoculum
Seed 10%H before kind2O2Immersion 10 minutes, deionized water is cleaned repeatedly and is drained several times), 2～3cm of earthing；
(7) spore density, Hyphal length that pedotheque carried out in detection soil etc. is taken after cultivating 3～6 months under normal management.
The present invention is achieved through the following technical solutions：
1 Glomus mosseae Glomus mossea fields expanding propagation method of example
(1) test material：
Corn seed (Glycyrrhiza uralensis Fisch), Glomus mosseae Glomus mossea microbial inoculums, are mopped up greatly.
(2) test method：
1) first all soil surfaces are removed totally with the root of crop, stem, leaf etc. in soil is buried in；
2) granule " will be mopped up greatly " to spread fertilizer over the fields in soil surface to be sterilized；
3) medicament is ploughed deeply to the 20～30cm of depth of soil of sterilization, smashes big soil block, be that the diffusion and infiltration of " mopping up greatly " decomposition gas is carried
For a good condition；
4) water to saturating, be close to soil surface and cover mulch film, be compacted around thin film, it is impossible to be ventilative；
5), after fumigating 7～10 days, it is ventilated 10～15 days to open thin film, will loosen the soil daily 1～2 time during ventilation；
6) after ventilated 10～15 days, arbuscular mycorrhiza microbial inoculum is sprinkled in soil surface, sprinkle Semen Maydiss again or the seed of Sorghum vulgare Pers. (is broadcast on microbial inoculum
Seed 10%H before kind2O2Immersion 10 minutes, deionized water is cleaned repeatedly and is drained several times), 2～3cm of earthing；
7) spore density, Hyphal length that pedotheque carried out in detection soil etc. is taken after cultivating 3～6 months under normal management.
(3) experimental result
In adopting the method expanding propagation to obtain soil, for 232～397/10, hyphae length is the spore density containing arbuscular mycorrhiza Glomus mosse
150～289.5 meters/10g.
2 Glomus versiforme Glomus versiforme fields expanding propagation method of example
(1) test material
Sorghum seeds seed (Glycyrrhiza uralensis Fisch), Glomus mosseae Glomus versiforme microbial inoculums, mop up greatly.
(2) test method：
1) first all soil surfaces are removed totally with the root of crop, stem, leaf etc. in soil is buried in；
2) granule " will be mopped up greatly " to spread fertilizer over the fields in soil surface to be sterilized；
3) medicament is ploughed deeply to the depth of soil 20-30cm of sterilization, smashes big soil block, be that the diffusion and infiltration of " mopping up greatly " decomposition gas is carried
For a good condition；
4) water to saturating, be close to soil surface and cover mulch film, be compacted around thin film, it is impossible to be ventilative；
5), after fumigating 7-10 days, it is ventilated 10-15 days to open thin film, will loosen the soil 1-2 time daily during ventilation；
6) after ventilated 10-15 days, arbuscular mycorrhiza microbial inoculum is sprinkled in soil surface, sprinkle the seed (sowing of Semen Maydiss or Sorghum vulgare Pers. on microbial inoculum again
Front seed 10%H2O2Immersion 10 minutes, deionized water is cleaned repeatedly and is drained several times), earthing 2-3cm
7) take after 3-6 month being cultivated under normal management spore density, Hyphal length that pedotheque carried out in detection soil etc..
(3) experimental result
In adopting the method expanding propagation to obtain soil, the spore density containing arbuscular mycorrhiza Glomus versiforme is for 278～352/10, hyphae length
For 153～264.2 meters/10g.
Claims (4)
1. a kind of method of AMF field high efficiency expanding propagation, its step is：
(1), before fumigating, all soil surfaces are removed totally with the root of crop, stem, leaf etc. in soil is buried in；
(2) granule " will be mopped up greatly " to spread fertilizer over the fields in soil surface to be sterilized；
(3) medicament is ploughed deeply to the 20～30cm of depth of soil of sterilization, smashes big soil block, the diffusion and infiltration for " mopping up greatly " decomposition gas provides a good condition；
(4) water to saturating, be close to soil surface and cover mulch film, be compacted around thin film, it is impossible to be ventilative；
(5), after fumigating 7～10 days, it is ventilated 10～15 days to open thin film, will loosen the soil daily 1～2 time during ventilation；
(6) after ventilated 10～15 days, arbuscular mycorrhiza microbial inoculum is sprinkled in soil surface, sprinkle the seed of Semen Maydiss or Sorghum vulgare Pers., 2～3cm of earthing on microbial inoculum again；
(7) spore density, Hyphal length that pedotheque carried out in detection soil etc., the 200/10g of spore density ＞ in soil, Hyphal length ＞ 150m/10g is taken after cultivating 3～6 months under normal management.
2. the method for requiring a kind of described AMF field high efficiency expanding propagation according to right 1, it is characterised in that：Described soil sterilant is to mop up greatly.
3. the method for requiring a kind of described AMF field high efficiency expanding propagation according to right 1 or right 2, it is characterised in that：Described AMF adopts Glomus mosseae Glomus mossea and Glomus versiforme Glomus versiforme.
4. the method for requiring a kind of described AMF field high efficiency expanding propagation according to right 1 or right 2 or right 3, it is characterised in that：Selected host plant is grass Semen Maydiss Zea mays L. and Sorghum vulgare Pers. Sorghum bicolor (L.) Moench.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107881113A (en) * | 2017-12-28 | 2018-04-06 | 三明市农业科学研究院 | A kind of long-term preservation method of mycorrhizal fungi India pyriform spore |
| CN109601248A (en) * | 2019-01-04 | 2019-04-12 | 三峡大学 | A kind of field expanding propagation method of arbuscular mycorrhizal fungi |
| CN109757329A (en) * | 2019-02-20 | 2019-05-17 | 山东省农业科学院生物技术研究中心 | A method of improving Peanut continuous cropping yield and quality |
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| CN103314676A (en) * | 2013-04-12 | 2013-09-25 | 宋福强 | Method for carrying out industrialized production on arbuscular mycorrhizal fungi agent |
| CN103125240B (en) * | 2013-03-06 | 2014-11-05 | 中国科学院南京土壤研究所 | Large-scale propagation technology for mycorrhiza fungi inoculum |
| CN103314943B (en) * | 2013-06-18 | 2015-05-27 | 中国矿业大学(北京) | Soil sterilization method for outdoor large-scale propagation of mycorrhizal fungi |
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2015
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Patent Citations (3)
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| CN103125240B (en) * | 2013-03-06 | 2014-11-05 | 中国科学院南京土壤研究所 | Large-scale propagation technology for mycorrhiza fungi inoculum |
| CN103314676A (en) * | 2013-04-12 | 2013-09-25 | 宋福强 | Method for carrying out industrialized production on arbuscular mycorrhizal fungi agent |
| CN103314943B (en) * | 2013-06-18 | 2015-05-27 | 中国矿业大学(北京) | Soil sterilization method for outdoor large-scale propagation of mycorrhizal fungi |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107881113A (en) * | 2017-12-28 | 2018-04-06 | 三明市农业科学研究院 | A kind of long-term preservation method of mycorrhizal fungi India pyriform spore |
| CN109601248A (en) * | 2019-01-04 | 2019-04-12 | 三峡大学 | A kind of field expanding propagation method of arbuscular mycorrhizal fungi |
| CN109757329A (en) * | 2019-02-20 | 2019-05-17 | 山东省农业科学院生物技术研究中心 | A method of improving Peanut continuous cropping yield and quality |
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