CN106501342A - Portable circular electrophoresis tank - Google Patents
Portable circular electrophoresis tank Download PDFInfo
- Publication number
- CN106501342A CN106501342A CN201611085167.8A CN201611085167A CN106501342A CN 106501342 A CN106501342 A CN 106501342A CN 201611085167 A CN201611085167 A CN 201611085167A CN 106501342 A CN106501342 A CN 106501342A
- Authority
- CN
- China
- Prior art keywords
- glue
- cell body
- electrophoresis tank
- outer tube
- inner core
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/453—Cells therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
Abstract
The invention discloses portable circular electrophoresis tank, including cell body and stripping fork, cell body is cylinder groove, it is sequentially placed inside cell body from the inside to the outside in glue inner core, in glue outer tube, cell body inner space is divided into three parts by two, respectively in glue outer tube and in the plastic tank between glue inner core, two buffering liquid grooves are placed with wound into the plastic sheeting of tubular in plastic tank.Two panels is bent in offset plate the anchor ring tubular structure being parallel to each other, while gel area is ensured, electrophoresis tank length and width is reduced, is easy to carry；Plastic sheeting and the cavity in formation placement gel media between glue inner core, film can carry gel media, be easy to taking-up and dyeing, the process of gel.The present invention can increase the area of plastic tank in the confined space, solve the problems, such as that electrophoresis slot device is not readily portable, due to electrophoretic apparatus many with circular design, therefore without difficult cleaning of dead angle, it is to avoid by the cross pollution between electrophoretic substance.
Description
Technical field
The present invention relates to electrophoretic techniques field, more particularly to portable circular electrophoresis tank.
Background technology
Electrophoresis in life science be a kind of conventional macromolecular substances (such as：Nucleic acid, protein etc.) detect and divide
From technology, it is widely used in the research field of each related discipline.Nucleotides, protein and polypeptide and amino acid etc. all have can
The group of ionization, each group can be absorbed or provide hydrogen ion in electrolyte solution, changed into as charged groups.In difference
In the presence of electrolyte and electric field, various materials can produce difference according to molecular size, institute's band net charge difference translational speed, from
And different types of molecule is separated from mixture.
Electrophoresis tank is to separate the usual means for identifying nucleic acid and protein ingredient, and existing electrophoresis slot device is broadly divided into disk
Electrophoresis tank, vertical slab electrophoresis groove, Horizontal electrophoresis tank, traditional electrophoretic apparatus are made up of two panels flat board, two panels flat board by glazing bar every
Open, gel is placed in middle space；Electrolytic cell includes the groove of electrophoretic apparatus and the stock solution positioned at electrophoretic apparatus both sides, in groove
Comprising electrode, solution is poured in two grooves, solution covers gel and electrode, and sample is added in the sample well of gel, be powered
Electrophoresis can be carried out afterwards.
The principle of gel electrophoresis is：When a kind of molecule is placed in the electric field, can be with certain according to the electrically charged property of institute
Speed shifts to appropriate electrode, and the mobility of the electrophoresis net charges entrained with electric-field intensity and electrophoresis molecule itself are into just
Than；Electric-field intensity is bigger, the net charge quantity entrained by electrophoresis molecule is more, and migration velocity is also faster.According to molecular size
Different, constitute or shape difference, and institute with net charge number, can by electrophoresis by protein or nucleic acid molecules from mixing
Separate in thing.
Classic flat-plate electrophoretic apparatus plate face takes up room larger, and plate face or so two ends and cell body connecting portion utilization rate are poor,
Cannot be reached with minimum electrophoresis sump volume and most effectively make full use of gel.Additionally, the electrophoresis tank of larger width takes up room
Larger, not readily portable and mobile.
Content of the invention
Present invention aim at, there is provided portable circular electrophoresis tank, with minimum electrophoresis sump volume, increase to greatest extent
Interior plastic tank using area, while solving the problems, such as that electrophoresis slot device is not readily portable.
For reaching above-mentioned purpose and required technical indicator, the present invention is solved the above problems by the following technical programs：
Portable circular electrophoresis tank, including cell body and stripping fork, the cell body is cylinder groove, inside cell body from the inside to the outside successively
Place in glue inner core and in glue outer tube, cell body inner space is divided into three parts by two；Above-mentioned in glue inner core be in glue outer tube
Between space be referred to as plastic tank, be buffer solution water jacket in space between glue outer tube and cell body, in glue inner core in in buffer solution
Groove.
The present invention does not change the advantage being parallel to each other in conventional flat electrophoresis tank in offset plate, solidifying to ensure that minimum volume is carried
Premised on glue surface product maximum, for the purpose of reducing the dimensions length of electrophoresis slot device as far as possible, two panels is bent to phase in offset plate
Mutually parallel anchor ring tubular structure, by being in glue inner core and being in that cell body is separated into three parts by glue outer tube, outermost is buffering
Liquid water jacket, middle part be for place the plastic tank of gel, inner side be buffer solution inside groove, possess the basic structure of electrophoresis tank.
Used as the improvement of the above-mentioned portable circular electrophoresis tank of the present invention, the cell body is furnished with the groove lid that mates with notch, groove
Lid is provided with the through hole for negative electrode insertion.Rod-shaped electrode treats the liquid level of buffering liquid in groove through in through hole insertion buffer solution inside groove
Do not cross negative pole lower end and cover notch, when plastic tank and buffer solution water jacket are connected, can be formed between positive and negative two electrode complete
Loop.
Used as the improvement of the above-mentioned portable circular electrophoresis tank of the present invention, the cell body includes cup dolly and is fixed on base
The hollow cylindrical cell wall structure on top, buffer solution water jacket are connected with anode by base.The groove of electrolytic cell both sides stock solution
Body should be connected with the both positive and negative polarity of power supply respectively, there is provided the electric field needed for molecular motion, buffer solution water jacket are connected by base and groove
The contrary electrode of the connected electrode of lid, meets the basic structure of electrophoresis tank.
Used as the improvement of the above-mentioned portable circular electrophoresis tank of the present invention, the base arranges the jack of fixed anode, buffering
The fixed fixture in glue outer tube is set in liquid water jacket, the platinum filament of conduction is set between jack and buffer solution water jacket；Connect with jack
The platinum filament for connecing enters buffer solution inside groove, and the fixture on the outside of in glue outer tube through base.Platinum wire ring is arranged around fixture
Conduction on circumferencial direction can be made uniform, so as to reach the purpose for improving electrophoresis efficiency.
As plastics of the placement wound into tubular in the improvement of the above-mentioned portable circular electrophoresis tank of the present invention, the plastic tank
Film.Plastic sheeting uniformly and closely can be affixed on the inwall in glue outer tube with its natural elasticity, and with glue inner core it
Between formed a cavity, this chamber provides a natural space for gel media.Meanwhile, plastic sheeting can carry running gel
Medium, gel when finishing the taking-up of rear gel and take out in order to electrophoresis are carried out again dyeing in the form of plane, are processed.And
The natural elasticity of plastic sheeting is clearly present its encapsulating space, does not interfere with the insertion of stripping fork.
As the improvement of the above-mentioned portable circular electrophoresis tank of the present invention, described in glue inner core bottom seal, described in glue outside
Cylinder bottom is through-hole structure.In glue inner core bottom seal, can ensure that buffer solution inside groove bottom is isolated with plastic tank, it is to avoid to electric charge
The motion of molecule produces interference.It is through-hole structure in glue outer tube bottom, is easy to passing through for electric current.
As the improvement of the above-mentioned portable circular electrophoresis tank of the present invention, described can with cell body in glue inner core and in glue outer tube from
By dismounting and connection, connecting portion is sealed by joint filling material.The taking-up for being easy to gel can be freely dismantled and connect, while
It is also beneficial to the cleaning for carrying plastic tank between gel two.For enabling encapsulating to be smoothed out, need before encapsulating by two cylinders with
The connecting portion sealing of cell body, makes electrophoretic separation medium in order to encapsulating.Remove sealing adhesive strip after glue condenses completely, so as to
When pouring electrophoresis liquid into and carrying out electrophoresis, electric current is passed through.
Used as the improvement of the above-mentioned portable circular electrophoresis tank of the present invention, the joint filling material adopts adhesive sticker.Adhesive sticker is
A kind of have larger stickiness, but the new category viscose glue for being difficult to solidify, and pastes thing with which, and having crossed for a long time also can be easily
Take off stripping.Position is detachably connected to two cylinders and base before encapsulating to seal, is easy to encapsulating smoothly can carry out；Gel
After condensation and before electrophoretic procedures, the adhesive sticker for sealing is removed, is easy to electrophoresis to be normally carried out.
Used as the improvement of the above-mentioned portable circular electrophoresis tank of the present invention, the comb handle of the stripping fork is bent into annulus, and tooth bar hangs down
Straight anchor ring is arranged.The shape of stripping fork should with plastic tank shape and carry the flux matched of sample, be solidifying using the purpose of stripping fork
The sawtooth sample well with gap is formed on glue, and when electrophoresis experiment is carried out, sample point sample is positioned in sample well.Stripping fork is inserted
The depth according to how many decisions of sample-adding amount, many insertions of amount are relatively deep, on the contrary shallow then.
Used as the improvement of the above-mentioned portable circular electrophoresis tank of the present invention, the cell body, groove lid, carrying glue inner core and outer tube are equal
Can adopt and make transparent with machine glass or corresponding material.Using transparent material such as lucite, electricity can be clearly viewed
The situation of each link during swimming, more intuitively understands that electrophoresis is produced and process.
The invention has the beneficial effects as follows：
1st, in the case that the present invention is parallel to each other advantage in conventional flat electrophoresis tank is not changed in offset plate, it is in glue by two panels
While plate benging ensures that in the anchor ring tubular structure being parallel to each other, unit volume gel area is maximum, electrophoresis tank dress is reduced
The dimensions length that puts.
2nd, wound is placed into the plastic sheeting of tubular, and plastic sheeting can be uniformly and tight with its natural elasticity in plastic tank
Be affixed on the inwall in glue outer tube, and with forming a cavity between glue inner core, for gel media provides one natural
Space.Plastic sheeting can carry gel media, and gel when finishing the taking-up of rear gel and take out in order to electrophoresis is again
Dyeed in the form of plane and processed.
3rd, in glue inner core and can freely dismantling with cell body and be connected in glue outer tube, in glue inner core bottom seal, in glue outer tube
Bottom adopts through hole, connecting portion to be sealed by joint filling material.Can freely dismantle and connect the taking-up of being easy to gel and put
The cleaning of glue groove.The design of two bottoms, and the use of joint filling material, are easy to being normally carried out for encapsulating and electrophoresis.
4th, this equipment separately available perform an analysis electrophoresis and prepare electrophoresis use, when electrophoresis tank adopt stripping fork less when, can
For use as analytical electrophoresis；When stripping fork is larger, can be used as preparing electrophoresis.
It is an object of the invention to provide portable circular electrophoresis tank, increases the area of plastic tank in the confined space, solve
The not readily portable problem of electrophoresis tank.
Description of the drawings
Fig. 1 is portable circular electrophoresis tank schematic diagram；
Fig. 2 is the profile of portable circular electrophoresis tank；
Fig. 3 is the exploded perspective schematic diagram of portable circular electrophoresis tank.
Figure number is identified：10th, cell body, 11, base, 111, keeper, 112 platinum filaments, 113, jack, 12, cell wall, 13, buffering
Liquid water jacket, 14, buffer solution inside groove, 15, plastic tank, 20, groove lid, 21, through hole, 22, putting hole, 30, stripping fork, 40, be in glue inner core,
50th, it is in glue outer tube, 60, plastic sheeting, 70, negative electrode, 80, anode.
Specific embodiment
The invention will be further described with reference to embodiments, but the invention is not limited in following examples.
Portable circular electrophoresis tank described in this example, as shown in Figures 1 and 2, including the cell body 10 and groove of cylinder
Lid 20, cell body 10 include cup dolly 11 and the hollow cylindrical cell wall 12 being fixed on base 11, and cell wall 12 is same with base 11
Axle.The groove lid 20 is the dome mated with the opening of cell body 10, and the lid of groove lid 20 is loaded on 10 top of cell body.
As shown in accompanying drawing 2 and accompanying drawing 3, two cylinders coaxial with base 11 inside cell body 10, are sequentially placed from the inside to the outside,
Near central axis cylinder be close in glue inner core 40 cell wall 12 cylinder be in glue outer tube 50, two barrels be parallel to each other and
Away from equal.With base 11 connecting portion by adhesive sticker bondd in glue inner core 40 and in glue outer tube 50, adhesive sticker is compared to remaining
Joint filling material has larger stickiness, is difficult the characteristics of solidifying, and more can take off stripping easily.
As shown in Figure 2, three independent sectors are separated into by parallel barrel inside cell body 10, center is buffer solution inside groove
14th, it is buffer solution water jacket 13 that middle part is plastic tank 15, outermost one is enclosed.Buffer solution inside groove 14 and buffer solution water jacket 13 are used for placing electricity
Solution liquid, plastic tank 15 can be used to prepare gel or places gel and sample.
As shown in Figure 3, wound is placed into the plastic sheeting 60 of tubular, and plastic sheeting 60 is with its natural bullet in plastic tank 15
Property uniformly and closely can be affixed on the inwall in glue outer tube, and with a cavity is formed between glue inner core 40, this chamber is
Gel media provides a natural space.Gel media is carried on plastic sheeting 60, and circular stripping fork 30 is inserted
Enter plastic sheeting 60 and in the cavity between glue inner core 40, and the zigzag sample with gap is formed on gel, and by sample
Product point sample is positioned in hole.The depth of the insertion of stripping fork 30 determines that according to sample-adding amount amount is at most deep, on the contrary shallow then.
As shown in Figure 3, in 50 bottom of glue outer tube be through hole, by four symmetrical keepers of 11 top center of base
111 are fixed on inside cell body 10, in 40 bottom seal of glue inner core, are positioned in glue outer tube 50.Cell body 10, groove lid 20, be in glue
Inner core 40, made using lucite in glue outer tube 50, the feelings of each link in electrophoresis process can clearly be observed by lucite
Condition, is conducive to control and the observation that tests.
Two buffering liquid grooves need to be connected with the two poles of the earth of opposite charge respectively, as shown in Figure 1, arrange at the edge of base 11
The jack 113 of jointed anode 80, jack 113 are connected by the platinum filament 112 through base 11 with fixture 111, and platinum filament 112 is the bottom of from
Seat 11 passes and winds 4 fixtures 111, in the incoming buffer solution water jacket 13 of the positive electricity that anode 80 is imported.And 20 center of groove lid
Axial location is provided with the through hole 21 mated with shaft-like negative electrode 70, and negative electrode 70 probes into buffer solution inside groove 14 through through hole 21, when in groove
During electrolyte contacts negative electrode 70, negative electrical charge can be conducted by electrolyte.20 edge of groove lid is provided with the putting hole 22 for placing negative electrode 70,
When electrophoresis is not carried out, negative electrode 70 can be placed and 20 edge of groove lid, it is to avoid pollution and loss.Because large biological molecule sample leads to
More negative electrical charge is carried often, is that the electrode that generally will be close to sample position is set to negative electrode 70 electrophoresis experiment is carried out.
Portable circular electrophoresis tank using method：When electrophoresis experiment is carried out, plastic sheeting 60 is put into plastic tank first
In 15, as the elasticity of plastic sheeting 60 makes which be close to put glue outer tube inner wall, a carrying gel media is formed between inner core
Cavity, the gel solution for preparing slowly is poured in cavity, and assembles stripping fork 30；After gel sets, by stripping fork 30 from
Extract out in groove, a circle zigzag sample well on gel, can be formed, sample is clicked and entered sample well using liquid-transfering gun and to record point sample suitable
Sequence and point sample amount；Buffer solution is slowly added in buffer solution inside groove 14, buffer solution covers buffer solution inside groove 14 and enters plastic tank
15 and buffer solution water jacket 13, buffer solution is added to liquid level did not had glue surface 1-1.5mm, finally connects two lateral electrodes and is tested.Core
The migration velocity of the macromoleculars such as thuja acid, protein and polypeptide and amino acid is directly proportional to electric-field intensity, can be mixed by electrolytic cell
The nucleic acid samples of conjunction carry out the separation of one-component under electric field action, reach the purpose being identified and isolated from.
Embodiments of the present invention are described in detail above in association with accompanying drawing, but the present invention is not limited to described embodiment party
Formula.For a person skilled in the art, without departing from the principles and spirit of the present invention to these embodiments
Carry out multiple changes, modification, replacement and modification to still fall within protection scope of the present invention.
Claims (10)
1. portable circular electrophoresis tank, including cell body (10) and stripping fork (30), it is characterised in that：Described cell body (10) are cylinder
It is sequentially placed inside groove, cell body (10) from the inside to the outside in glue inner core (40), in glue outer tube (50), two will be internal for cell body (10) empty
Between be divided into three parts；Described is plastic tank (15) in glue outer tube (50) and in the space between glue inner core (40), in glue outer tube
(50) space is buffer solution water jacket (13) and cell body (10) between, and interior in glue inner core (40) is buffer solution inside groove (14).
2. portable circular electrophoresis tank according to claim 1, it is characterised in that：Cell body (10) are furnished with and notch
The groove lid (20) that matches somebody with somebody, groove lid (20) are provided with the through hole inserted for negative electrode (70).
3. portable circular electrophoresis tank according to claim 1, it is characterised in that：Described cell body (10) include cup dolly
(11) and hollow cylindrical cell wall (12) structure on base (11) top is fixed on；Described buffer solution water jacket (13) pass through base
(11) it is connected with anode (80).
4. portable circular electrophoresis tank according to claim 3, it is characterised in that：Described base (11) arrange fixed anode
Jack (113), the fixed fixture (111) in glue outer tube (50) is set in buffer solution water jacket (13), is connected with jack (113)
Platinum filament (112) enter buffer solution water jacket (13) through base (11), and around in the fixture on the outside of glue outer tube (50)
(111).
5. portable circular electrophoresis tank according to claim 1, it is characterised in that：Wound is placed in plastic tank (15)
Plastic sheeting (60) into tubular.
6. portable circular electrophoresis tank according to claim 1, it is characterised in that：Described in glue inner core (40) bottom seal
Mouthful, it is through-hole structure in glue outer tube (50) bottom.
7. portable circular electrophoresis tank according to claim 1, it is characterised in that：Described in glue inner core (40), in outside glue
Cylinder (50) is detachably connected with cell body (10), and connecting portion is sealed by joint filling material.
8. portable circular electrophoresis tank according to claim 7, it is characterised in that：The joint filling material adopts adhesive sticker.
9. portable circular electrophoresis tank according to claim 1, it is characterised in that：Described stripping fork (30) are combed for ring-type, tooth
The vertical comb handle of bar is arranged.
10. the portable circular electrophoresis tank according to claim 1～9 any one, it is characterised in that：The cell body
(10), groove lid (20), in glue inner core (40), made using lucite in glue outer tube (50).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611085167.8A CN106501342B (en) | 2016-11-30 | 2016-11-30 | Portable circle electrophoresis tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611085167.8A CN106501342B (en) | 2016-11-30 | 2016-11-30 | Portable circle electrophoresis tank |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106501342A true CN106501342A (en) | 2017-03-15 |
| CN106501342B CN106501342B (en) | 2019-04-30 |
Family
ID=58327304
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611085167.8A Expired - Fee Related CN106501342B (en) | 2016-11-30 | 2016-11-30 | Portable circle electrophoresis tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106501342B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5024749A (en) * | 1990-04-20 | 1991-06-18 | Westinghouse Electric Corp. | System and method for continuous separation of isotopes |
| US5183548A (en) * | 1990-04-20 | 1993-02-02 | Westinghouse Electric Corp. | System and method for continuous separation of isotopes |
| CN101614695A (en) * | 2009-07-23 | 2009-12-30 | 杭州吉来生物技术有限公司 | A kind of integral electrophoretic apparatus that is used for gel electrophoresis |
| CN104415661A (en) * | 2013-09-09 | 2015-03-18 | 杜权 | Separation technique of biological macromolecules as well as application thereof |
| CN206219696U (en) * | 2016-11-30 | 2017-06-06 | 桂林医学院 | Portable circular electrophoresis tank |
-
2016
- 2016-11-30 CN CN201611085167.8A patent/CN106501342B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5024749A (en) * | 1990-04-20 | 1991-06-18 | Westinghouse Electric Corp. | System and method for continuous separation of isotopes |
| US5183548A (en) * | 1990-04-20 | 1993-02-02 | Westinghouse Electric Corp. | System and method for continuous separation of isotopes |
| CN101614695A (en) * | 2009-07-23 | 2009-12-30 | 杭州吉来生物技术有限公司 | A kind of integral electrophoretic apparatus that is used for gel electrophoresis |
| CN104415661A (en) * | 2013-09-09 | 2015-03-18 | 杜权 | Separation technique of biological macromolecules as well as application thereof |
| CN206219696U (en) * | 2016-11-30 | 2017-06-06 | 桂林医学院 | Portable circular electrophoresis tank |
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| Publication number | Publication date |
|---|---|
| CN106501342B (en) | 2019-04-30 |
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