CN106498008A - The preparation method of superhigh maltose syrup of three enzymatic conversion of enzyme ginseng raw material pre-treatment - Google Patents
The preparation method of superhigh maltose syrup of three enzymatic conversion of enzyme ginseng raw material pre-treatment Download PDFInfo
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- CN106498008A CN106498008A CN201611084459.XA CN201611084459A CN106498008A CN 106498008 A CN106498008 A CN 106498008A CN 201611084459 A CN201611084459 A CN 201611084459A CN 106498008 A CN106498008 A CN 106498008A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/22—Preparation of compounds containing saccharide radicals produced by the action of a beta-amylase, e.g. maltose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K7/00—Maltose
Abstract
The present invention relates to a kind of preparation method of superhigh maltose syrup of three enzymatic conversion of enzyme ginseng raw material pre-treatment, its ratio meter by weight, it is made up of following raw material, takes off 16～20 parts of embryo corn, 4～6 parts of tartaric acid, 4～6 parts of 18% aqueous sodium carbonate, 3～5 parts of adsorbent, 3～5 parts of flocculant；Its preparation method is as follows, takes off ferment treatment of extruding of embryo corn；The secondary ferment treatment of the liquefaction of cornstarch；The saccharification of three enzyme process of enzyme is generated by the beta amylase of cornstarch, Pullulanase and maltose；Saccharification feed liquid sterilizing；Sterile malt sugar material liquid removal of impurities；Cleaning sterile maltose feed liquid is purified；Pure maltose feed liquid concentration.Pre-treatment of the present invention through double participations twice, substantially increase the conversion ratio of raw material, high maltose inversion quantity is obtained during three enzymatic conversions for lengthening the reaction time, and control accessory substance growing amount in purge process in extremely low level, raw material low cost and easily buying, require to the transformation of common apparatus low.
Description
Technical field
The invention belongs to the production technical field of malt syrup, more particularly to a kind of three enzymatic conversion of enzyme ginseng raw material pre-treatment
Preparation method of superhigh maltose syrup.
Background technology
Malt syrup is with high-grade maize starch as raw material, and the sugar based on maltose is obtained through multiple enzyme hydrolysis
Slurry, the product is a kind of sticky liquid of water white transparency, and plastid is limpid, transparent, mouthfeel is gently pure, low sugariness, has malt fragrant
Taste, with infusion temperature high, freezing point is low, resistive connection is brilliant many advantages, such as, be commonly used among jam, jelly, also can be used for bread,
In cake, beer, while being also widely used for the fields such as candy, beverage, food processed, frozen food, flavouring.Due to malt
Syrup is independent of human pancreatic islet metabolism, and blood sugar rises slowly, and caloric value is low, to cardiovascular patient, diabetes patient and obesity
Person has certain health care.The traditional preparation methods of malt syrup are typically prepared by cereal starch, raw saccharified, syrup purification
Constitute etc. step, and by as far as possible improve in saccharified liquid the content of maltose itself and reduce in malt syrup beyond maltose into
The monose for dividing and the method for oligosaccharide content improve the content ratio of maltose in obtained malt syrup.Chinese invention patent
(CN102766666) a kind of preparation method of superhigh maltose syrup is provided, maltodextrin in product is controlled by three enzyme process
Low DE values and reduce the content of glucose, but its using the traditional processing method of cereal starch raw material make to which defines whole product
The extreme value that malt syrup content can reach in thing.Chinese invention patent (103695503 B of CN) discloses a kind of height and endures warm malt
The preparation method of syrup, by sizing mixing, liquefying, the step such as high temperature filtration, filter at low temperature pre-treatment is carried out to raw material, although break through
Restriction of the traditional raw material processing method to end-product maltose content, but this pre-treating method technique is excessively complicated, to existing
The transformation for having equipment has high demands, and the temperature difference that high/low temperature is filtered is greatly, and transfer process is easy to destroy dividing for maltose itself
Sub- configuration and structure bond energy, cause end-product small molecular accessory substance to increase.It is thus desirable to breaking through material processing to end
On the basis of product maltose content is limited, find the preparation side that technique is more feasible sample syrup is bought with the high-load of practicality
Method.
Content of the invention
The technical problem to be solved in the present invention is the drawbacks described above for how overcoming prior art, there is provided before a kind of enzyme ginseng raw material
Process the preparation method of superhigh maltose syrup of three enzymatic conversions.
For solving above-mentioned technical problem, the preparation method of superhigh maltose syrup of three enzymatic conversion of this enzyme ginseng raw material pre-treatment is pressed
Weight ratio meter, is made up of following raw material, take off 16～20 parts of embryo corn, 4～6 parts of tartaric acid, 18% aqueous sodium carbonate 4～
6 parts, 3～5 parts of adsorbent, 3～5 parts of flocculant；Its concrete preparation method is comprised the following steps, and step (1) is by de- embryo corn
Ferment treatment of extruding cornstarch siccative is obtained；Step (2) is obtained liquefaction by the secondary ferment treatment of the liquefaction of cornstarch
Cornstarch；Step (3) generates the saccharification of three enzyme process of enzyme by the beta amylase of cornstarch, Pullulanase and maltose and sugar is obtained
Material liquid；Step (4) is obtained sterile malt sugar material liquid by the feed liquid sterilizing that is saccharified；Step (5) is removed by sterile malt sugar material liquid
Miscellaneous prepared cleaning sterile maltose feed liquid；Step (6) is obtained pure maltose solution by cleaning sterile maltose feed liquid purifying；
Step (7) is obtained this high-load malt syrup by the concentration of pure maltose feed liquid.
Further, ferment treatment of extruding of the de- embryo corn described in step (1) is specifically, under normal temperature, take off to described
Mesophilicα-diastase and resistance to middle temperature amylase is added in embryo corn, extrudes, cornstarch siccative is obtained in input extruder.
Further, the secondary ferment treatment of the liquefaction of the cornstarch described in step (2) is specifically, by cornstarch siccative weight
The deionized water of 2～3 times of amount pours material-compound tank into, adds high-temperatureα-amylase and fire resistant alpha-diastase, adds the winestone
The pH value of mixed liquor is adjusted to 6.2 by acid, and water temperature is adjusted to 60 DEG C, is then poured into cornstarch siccative obtained in step (1)
Material-compound tank, material-compound tank is brought rapidly up to 80 DEG C, and stirring obtains liquefied corn starch to liquefying completely in 15 minutes.
Further, the beta amylase of the cornstarch described in step (3), Pullulanase and maltose generate three enzyme process of enzyme
Saccharification is specifically, by liquefied corn starch feeding saccharifying tank obtained in step (2), adding remaining tartaric acid while stirring, inciting somebody to action
In saccharifying tank, the pH value of liquefied corn starch is adjusted to as 5.8, and temperature is adjusted to 70 DEG C, and it is β-starch that stirring adds carbohydrase
Enzyme, Pullulanase and maltose generate enzyme reaction reaction in 4 hours to be terminated, and adds 18% appropriate aqueous sodium carbonate, will saccharification
In tank, the pH value of liquid is adjusted to 7, is incubated a few hours, and the maltose content after saccharification reaches desired value, and iodine inspection is qualified, must be saccharified
Feed liquid.
Further, described in step (4) saccharification feed liquid sterilizing is specifically, use in the obtained saccharification feed liquid of step (3)
Absolute ethyl alcohol does saccharification detection, and in the presence of feed liquid is without dextrin, saccharifying tank is heated to 90 DEG C, and is incubated 1 hour, is then cooled to 60
DEG C, 2 hours are stood, sterile malt sugar material liquid is obtained.
Further, described in step (5) sterile malt sugar material liquid removal of impurities is specifically, by aseptic wheat obtained in step (4)
Bud sugar material liquid puts into decolouring kettle, and adds the adsorbent, flocculant, and decolouring kettle temperature degree is quickly raised to 80 DEG C, and stirring 1 is little
Shi Hou, and 4 hours are stood, impurity and flocculate are filtered by filter by the liquid in decolouring kettle, cleaning sterile malt is obtained
Sugar material liquid.
Further, the cleaning sterile maltose feed liquid purifying described in step (6) is specifically, by nothing obtained in step (5)
The input resin anion (R.A.) adsorption tanks absorption of bacterium maltose feed liquid, then by absorption after feed liquid send into resin cation adsorption tanks and inhale
Attached, after zwitterion absorption, obtain pure maltose solution.
Further, described in step (7) pure maltose feed liquid concentration is specifically, by maltose pure for step (6)
Solution puts into concentration kettle, discharges, obtain final product this high-load malt syrup after being concentrated into water content less than 15%.
Used as optimization, the adsorbent is one or more kinds of in activated carbon, silica gel or zeolite molecular sieve
Mixture.
Used as optimization, the flocculant is one or more kinds of in food grade polyacrylamide or shitosan
Mixture.
The preparation method of superhigh maltose syrup of three enzymatic conversion of enzyme ginseng raw material of the present invention pre-treatment is with de- embryo corn as original material
Material, not only reduces cost of material, controls material quality, and through extruding, liquefaction twice double enzymes participate in high pressure homogenizations and
The pre-treatment step that continuous injection is participated in, substantially increases the conversion ratio and purifying of raw material maltose in follow-up saccharification step
The conversion stability of maltose in step, converting to high maltose during three enzymatic conversions for lengthening the reaction time
Amount, and accessory substance growing amount in purge process is controlled in extremely low level；Using tartaric acid, sodium acid carbonate, adsorbent, flocculation
Agent is auxiliary material, and cost of material is low and easy buying；Using material-compound tank, saccharifying tank, decolouring kettle, filter, zwitterion absorption
Tank, concentration kettle are Preparation equipment, the transformation of existing common apparatus are required low；And production process is from basic material to final product
There is no waste, pollution and waste discharge；It is widely used in as intermediate malt syrup, the production of enzyme preparation, and candy,
The finished product food service industry such as beer, cold drink, jelly, dairy products, meat products.
Description of the drawings
Below in conjunction with the accompanying drawings enzyme of the present invention is joined three enzymatic conversion of raw material pre-treatment preparation method of superhigh maltose syrup make into
One step explanation：
Fig. 1 is the technological process line frame graph of the preparation method of superhigh maltose syrup of three enzymatic conversion of this enzyme ginseng raw material pre-treatment.
Specific embodiment
Embodiment 1：As shown in figure 1, ratio meter by weight, takes 20 parts of de- embryo corn, 4 parts of tartaric acid, 18% sodium carbonate
6 parts of the aqueous solution, 3 parts of zeolite molecular sieve, 5 parts of shitosan；
(1), under normal temperature, mesophilicα-diastase and resistance to middle temperature amylase are added, in input extruder in the de- embryo corn
Extruding, is obtained cornstarch siccative；
(2) pour the deionized water of 2～3 times of cornstarch siccative weight into material-compound tank, add high-temperatureα-amylase and
Fire resistant alpha-diastase, adds the tartaric acid, and the pH value of mixed liquor is adjusted to 6.2, and water temperature is adjusted to 60 DEG C, then will step
Suddenly obtained in (1), material-compound tank poured into by cornstarch siccative, and material-compound tank is brought rapidly up to 80 DEG C, stirs 15 minutes to complete liquid
Change, obtain liquefied corn starch；
(3) liquefied corn starch obtained in step (2) is sent into saccharifying tank, adds remaining tartaric acid while stirring, will
In saccharifying tank, the pH value of liquefied corn starch is adjusted to as 5.8, and temperature is adjusted to 70 DEG C, and it is β-starch that stirring adds carbohydrase
Enzyme, Pullulanase and maltose generate enzyme reaction reaction in 4 hours to be terminated, and adds 18% appropriate aqueous sodium carbonate, will saccharification
In tank, the pH value of liquid is adjusted to 7, is incubated a few hours, and the maltose content after saccharification reaches desired value, and iodine inspection is qualified, must be saccharified
Feed liquid；
(4) saccharification detection, in the presence of feed liquid is without dextrin, sugar are done with absolute ethyl alcohol in the obtained saccharification feed liquid of step (3)
Change tank and be heated to 90 DEG C, and be incubated 1 hour, be then cooled to 60 DEG C, stand 2 hours, obtain sterile malt sugar material liquid；
(5) by sterile malt sugar material liquid input decolouring kettle obtained in step (4), and the activated carbon, food-grade is added to gather
Decolouring kettle temperature degree is quickly raised to 80 DEG C by acrylamide, after stirring 1 hour, and stands 4 hours, by the liquid in decolouring kettle
Impurity and flocculate are filtered by filter, obtain cleaning sterile maltose feed liquid；
(6) by sterile malt sugar material liquid input resin anion (R.A.) adsorption tanks absorption obtained in step (5), after then adsorbing
Feed liquid send into the absorption of resin cation adsorption tanks, after zwitterion absorption, obtain pure maltose solution；
(7) by maltose solution input concentration kettle pure for step (6), discharge after being concentrated into water content less than 15%, i.e.,
Obtain this high-load malt syrup.
Embodiment 2：Ratio meter by weight, take 16 parts of de- embryo corn, 6 parts of tartaric acid, 4 parts of 18% aqueous sodium carbonate,
5 parts of zeolite molecular sieve, 3 parts of shitosan；
(1), under normal temperature, mesophilicα-diastase and resistance to middle temperature amylase are added, in input extruder in the de- embryo corn
Extruding, is obtained cornstarch siccative；
(2) pour the deionized water of 2～3 times of cornstarch siccative weight into material-compound tank, add high-temperatureα-amylase and
Fire resistant alpha-diastase, adds the tartaric acid, and the pH value of mixed liquor is adjusted to 6.2, and water temperature is adjusted to 60 DEG C, then will step
Suddenly obtained in (1), material-compound tank poured into by cornstarch siccative, and material-compound tank is brought rapidly up to 80 DEG C, stirs 15 minutes to complete liquid
Change, obtain liquefied corn starch；
(3) liquefied corn starch obtained in step (2) is sent into saccharifying tank, adds remaining tartaric acid while stirring, will
In saccharifying tank, the pH value of liquefied corn starch is adjusted to as 5.8, and temperature is adjusted to 70 DEG C, and it is β-starch that stirring adds carbohydrase
Enzyme, Pullulanase and maltose generate enzyme reaction reaction in 4 hours to be terminated, and adds 18% appropriate aqueous sodium carbonate, will saccharification
In tank, the pH value of liquid is adjusted to 7, is incubated a few hours, and the maltose content after saccharification reaches desired value, and iodine inspection is qualified, must be saccharified
Feed liquid；
(4) saccharification detection, in the presence of feed liquid is without dextrin, sugar are done with absolute ethyl alcohol in the obtained saccharification feed liquid of step (3)
Change tank and be heated to 90 DEG C, and be incubated 1 hour, be then cooled to 60 DEG C, stand 2 hours, obtain sterile malt sugar material liquid；
(5) by sterile malt sugar material liquid input decolouring kettle obtained in step (4), and the activated carbon, food-grade is added to gather
Decolouring kettle temperature degree is quickly raised to 80 DEG C by acrylamide, after stirring 1 hour, and stands 4 hours, by the liquid in decolouring kettle
Impurity and flocculate are filtered by filter, obtain cleaning sterile maltose feed liquid；
(6) by sterile malt sugar material liquid input resin anion (R.A.) adsorption tanks absorption obtained in step (5), after then adsorbing
Feed liquid send into the absorption of resin cation adsorption tanks, after zwitterion absorption, obtain pure maltose solution；
(7) by maltose solution input concentration kettle pure for step (6), discharge after being concentrated into water content less than 15%, i.e.,
Obtain this high-load malt syrup.
Embodiment 3：Ratio meter by weight, take 18 parts of de- embryo corn, 5 parts of tartaric acid, 5 parts of 18% aqueous sodium carbonate,
4 parts of zeolite molecular sieve, 4 parts of shitosan；
(1), under normal temperature, mesophilicα-diastase and resistance to middle temperature amylase are added, in input extruder in the de- embryo corn
Extruding, is obtained cornstarch siccative；
(2) pour the deionized water of 2～3 times of cornstarch siccative weight into material-compound tank, add high-temperatureα-amylase and
Fire resistant alpha-diastase, adds the tartaric acid, and the pH value of mixed liquor is adjusted to 6.2, and water temperature is adjusted to 60 DEG C, then will step
Suddenly obtained in (1), material-compound tank poured into by cornstarch siccative, and material-compound tank is brought rapidly up to 80 DEG C, stirs 15 minutes to complete liquid
Change, obtain liquefied corn starch；
(3) liquefied corn starch obtained in step (2) is sent into saccharifying tank, adds remaining tartaric acid while stirring, will
In saccharifying tank, the pH value of liquefied corn starch is adjusted to as 5.8, and temperature is adjusted to 70 DEG C, and it is β-starch that stirring adds carbohydrase
Enzyme, Pullulanase and maltose generate enzyme reaction reaction in 4 hours to be terminated, and adds 18% appropriate aqueous sodium carbonate, will saccharification
In tank, the pH value of liquid is adjusted to 7, is incubated a few hours, and the maltose content after saccharification reaches desired value, and iodine inspection is qualified, must be saccharified
Feed liquid；
(4) saccharification detection, in the presence of feed liquid is without dextrin, sugar are done with absolute ethyl alcohol in the obtained saccharification feed liquid of step (3)
Change tank and be heated to 90 DEG C, and be incubated 1 hour, be then cooled to 60 DEG C, stand 2 hours, obtain sterile malt sugar material liquid；
(5) by sterile malt sugar material liquid input decolouring kettle obtained in step (4), and the activated carbon, food-grade is added to gather
Decolouring kettle temperature degree is quickly raised to 80 DEG C by acrylamide, after stirring 1 hour, and stands 4 hours, by the liquid in decolouring kettle
Impurity and flocculate are filtered by filter, obtain cleaning sterile maltose feed liquid；
(6) by sterile malt sugar material liquid input resin anion (R.A.) adsorption tanks absorption obtained in step (5), after then adsorbing
Feed liquid send into the absorption of resin cation adsorption tanks, after zwitterion absorption, obtain pure maltose solution；
(7) by maltose solution input concentration kettle pure for step (6), discharge after being concentrated into water content less than 15%, i.e.,
Obtain this high-load malt syrup.
Embodiment 4：Ratio meter by weight, take 18 parts of de- embryo corn, 5 parts of tartaric acid, 5 parts of 18% aqueous sodium carbonate,
4 parts of activated carbon, 4 parts of food grade polyacrylamide；
(1), under normal temperature, mesophilicα-diastase and resistance to middle temperature amylase are added, in input extruder in the de- embryo corn
Extruding, is obtained cornstarch siccative；
(2) pour the deionized water of 2～3 times of cornstarch siccative weight into material-compound tank, add high-temperatureα-amylase and
Fire resistant alpha-diastase, adds the tartaric acid, and the pH value of mixed liquor is adjusted to 6.2, and water temperature is adjusted to 60 DEG C, then will step
Suddenly obtained in (1), material-compound tank poured into by cornstarch siccative, and material-compound tank is brought rapidly up to 80 DEG C, stirs 15 minutes to complete liquid
Change, obtain liquefied corn starch；
(3) liquefied corn starch obtained in step (2) is sent into saccharifying tank, adds remaining tartaric acid while stirring, will
In saccharifying tank, the pH value of liquefied corn starch is adjusted to as 5.8, and temperature is adjusted to 70 DEG C, and it is β-starch that stirring adds carbohydrase
Enzyme, Pullulanase and maltose generate enzyme reaction reaction in 4 hours to be terminated, and adds 18% appropriate aqueous sodium carbonate, will saccharification
In tank, the pH value of liquid is adjusted to 7, is incubated a few hours, and the maltose content after saccharification reaches desired value, and iodine inspection is qualified, must be saccharified
Feed liquid；
(4) saccharification detection, in the presence of feed liquid is without dextrin, sugar are done with absolute ethyl alcohol in the obtained saccharification feed liquid of step (3)
Change tank and be heated to 90 DEG C, and be incubated 1 hour, be then cooled to 60 DEG C, stand 2 hours, obtain sterile malt sugar material liquid；
(5) by sterile malt sugar material liquid input decolouring kettle obtained in step (4), and the activated carbon, food-grade is added to gather
Decolouring kettle temperature degree is quickly raised to 80 DEG C by acrylamide, after stirring 1 hour, and stands 4 hours, by the liquid in decolouring kettle
Impurity and flocculate are filtered by filter, obtain cleaning sterile maltose feed liquid；
(6) by sterile malt sugar material liquid input resin anion (R.A.) adsorption tanks absorption obtained in step (5), after then adsorbing
Feed liquid send into the absorption of resin cation adsorption tanks, after zwitterion absorption, obtain pure maltose solution；
(7) by maltose solution input concentration kettle pure for step (6), discharge after being concentrated into water content less than 15%, i.e.,
Obtain this high-load malt syrup.
Embodiment 5：Ratio meter by weight, take 18 parts of de- embryo corn, 5 parts of tartaric acid, 5 parts of 18% aqueous sodium carbonate,
4 parts of silica gel, 4 parts of shitosan；
(1), under normal temperature, mesophilicα-diastase and resistance to middle temperature amylase are added, in input extruder in the de- embryo corn
Extruding, is obtained cornstarch siccative；
(2) pour the deionized water of 2～3 times of cornstarch siccative weight into material-compound tank, add high-temperatureα-amylase and
Fire resistant alpha-diastase, adds the tartaric acid, and the pH value of mixed liquor is adjusted to 6.2, and water temperature is adjusted to 60 DEG C, then will step
Suddenly obtained in (1), material-compound tank poured into by cornstarch siccative, and material-compound tank is brought rapidly up to 80 DEG C, stirs 15 minutes to complete liquid
Change, obtain liquefied corn starch；
(3) liquefied corn starch obtained in step (2) is sent into saccharifying tank, adds remaining tartaric acid while stirring, will
In saccharifying tank, the pH value of liquefied corn starch is adjusted to as 5.8, and temperature is adjusted to 70 DEG C, and it is β-starch that stirring adds carbohydrase
Enzyme, Pullulanase and maltose generate enzyme reaction reaction in 4 hours to be terminated, and adds 18% appropriate aqueous sodium carbonate, will saccharification
In tank, the pH value of liquid is adjusted to 7, is incubated a few hours, and the maltose content after saccharification reaches desired value, and iodine inspection is qualified, must be saccharified
Feed liquid；
(4) saccharification detection, in the presence of feed liquid is without dextrin, sugar are done with absolute ethyl alcohol in the obtained saccharification feed liquid of step (3)
Change tank and be heated to 90 DEG C, and be incubated 1 hour, be then cooled to 60 DEG C, stand 2 hours, obtain sterile malt sugar material liquid；
(5) by sterile malt sugar material liquid input decolouring kettle obtained in step (4), and the silica gel, shitosan are added, will be de-
Color kettle temperature degree is quickly raised to 80 DEG C, after stirring 1 hour, and stands 4 hours, will be miscellaneous by filter by the liquid in decolouring kettle
Matter and flocculate are filtered, and obtain cleaning sterile maltose feed liquid；
(6) by sterile malt sugar material liquid input resin anion (R.A.) adsorption tanks absorption obtained in step (5), after then adsorbing
Feed liquid send into the absorption of resin cation adsorption tanks, after zwitterion absorption, obtain pure maltose solution；
(7) by maltose solution input concentration kettle pure for step (6), discharge after being concentrated into water content less than 15%, i.e.,
Obtain this high-load malt syrup.
Embodiment 6：Ratio meter by weight, take 18 parts of de- embryo corn, 5 parts of tartaric acid, 5 parts of 18% aqueous sodium carbonate,
4 parts of silica gel, 4 parts of food grade polyacrylamide；
(1), under normal temperature, mesophilicα-diastase and resistance to middle temperature amylase are added, in input extruder in the de- embryo corn
Extruding, is obtained cornstarch siccative；
(2) pour the deionized water of 2～3 times of cornstarch siccative weight into material-compound tank, add high-temperatureα-amylase and
Fire resistant alpha-diastase, adds the tartaric acid, and the pH value of mixed liquor is adjusted to 6.2, and water temperature is adjusted to 60 DEG C, then will step
Suddenly obtained in (1), material-compound tank poured into by cornstarch siccative, and material-compound tank is brought rapidly up to 80 DEG C, stirs 15 minutes to complete liquid
Change, obtain liquefied corn starch；
(3) liquefied corn starch obtained in step (2) is sent into saccharifying tank, adds remaining tartaric acid while stirring, will
In saccharifying tank, the pH value of liquefied corn starch is adjusted to as 5.8, and temperature is adjusted to 70 DEG C, and it is β-starch that stirring adds carbohydrase
Enzyme, Pullulanase and maltose generate enzyme reaction reaction in 4 hours to be terminated, and adds 18% appropriate aqueous sodium carbonate, will saccharification
In tank, the pH value of liquid is adjusted to 7, is incubated a few hours, and the maltose content after saccharification reaches desired value, and iodine inspection is qualified, must be saccharified
Feed liquid；
(4) saccharification detection, in the presence of feed liquid is without dextrin, sugar are done with absolute ethyl alcohol in the obtained saccharification feed liquid of step (3)
Change tank and be heated to 90 DEG C, and be incubated 1 hour, be then cooled to 60 DEG C, stand 2 hours, obtain sterile malt sugar material liquid；
(5) by sterile malt sugar material liquid input decolouring kettle obtained in step (4), and the silica gel, food-grade poly- third is added
Decolouring kettle temperature degree is quickly raised to 80 DEG C by acrylamide, after stirring 1 hour, and stands 4 hours, the liquid in decolouring kettle is led to
Cross filter to filter impurity and flocculate, obtain cleaning sterile maltose feed liquid；
(6) by sterile malt sugar material liquid input resin anion (R.A.) adsorption tanks absorption obtained in step (5), after then adsorbing
Feed liquid send into the absorption of resin cation adsorption tanks, after zwitterion absorption, obtain pure maltose solution；
(7) by maltose solution input concentration kettle pure for step (6), discharge after being concentrated into water content less than 15%, i.e.,
Obtain this high-load malt syrup.
It can be that professional and technical personnel in the field realize or use that above-mentioned embodiment is intended to illustrate the present invention, to above-mentioned
Embodiment is modified, therefore the present invention is included but is not limited to
Above-mentioned embodiment, any meet the claims or specification description, meet with principles disclosed herein and novelty,
The method of inventive features, technique, product, each fall within protection scope of the present invention.
Claims (10)
1. a kind of preparation method of superhigh maltose syrup of three enzymatic conversion of enzyme ginseng raw material pre-treatment, is characterized in that：Ratio by weight
Meter, is made up of following raw material, takes off 16～20 parts of embryo corn, 4～6 parts of tartaric acid, 4～6 parts of 18% aqueous sodium carbonate, adsorbent
3～5 parts, 3～5 parts of flocculant；Its concrete preparation method is comprised the following steps, step (1) by the extruding that takes off embryo corn once
Ferment treatment is obtained cornstarch siccative；Step (2) is obtained liquefied corn starch by the secondary ferment treatment of the liquefaction of cornstarch；Step
Suddenly (3) generate the saccharification of three enzyme process of enzyme by the beta amylase of cornstarch, Pullulanase and maltose and saccharification feed liquid are obtained；Step
(4) sterile malt sugar material liquid is obtained by the feed liquid sterilizing that is saccharified；Step (5) is obtained cleaning nothing by the removal of impurities of sterile malt sugar material liquid
Bacterium maltose feed liquid；Step (6) is obtained pure maltose solution by cleaning sterile maltose feed liquid purifying；Step (7) passes through
Pure maltose feed liquid concentration is obtained this high-load malt syrup.
2. enzyme according to claim 1 joins the preparation method of superhigh maltose syrup of three enzymatic conversion of raw material pre-treatment, its feature
It is：Ferment treatment of extruding of de- embryo corn described in step (1) specifically, under normal temperature, in adding in the de- embryo corn
Warm AMS and resistance to middle temperature amylase, extrude in input extruder, cornstarch siccative are obtained.
3. enzyme according to claim 1 joins the preparation method of superhigh maltose syrup of three enzymatic conversion of raw material pre-treatment, its feature
It is：The secondary ferment treatment of liquefaction of the cornstarch described in step (2) specifically, by 2～3 times of cornstarch siccative weight go from
Sub- water pours material-compound tank into, adds high-temperatureα-amylase and fire resistant alpha-diastase, adds the tartaric acid, by the pH of mixed liquor
Value is adjusted to 6.2, and water temperature is adjusted to 60 DEG C, then pours cornstarch siccative obtained in step (1) into material-compound tank, by material-compound tank
It is brought rapidly up to 80 DEG C, stirring obtains liquefied corn starch to liquefying completely in 15 minutes.
4. enzyme according to claim 1 joins the preparation method of superhigh maltose syrup of three enzymatic conversion of raw material pre-treatment, its feature
It is：The beta amylase of the cornstarch described in step (3), Pullulanase and maltose generate the saccharification of three enzyme process of enzyme specifically, will
Liquefied corn starch obtained in step (2) sends into saccharifying tank, adds remaining tartaric acid while stirring, beautiful by liquefying in saccharifying tank
The pH value of rice starch is adjusted to as 5.8, and temperature is adjusted to 70 DEG C, and it is beta amylase, Pullulanase and wheat that stirring adds carbohydrase
Bud sugar generates enzyme reaction reaction in 4 hours to be terminated, and adds 18% appropriate aqueous sodium carbonate, the pH value of liquid in saccharifying tank is adjusted
Whole is 7, is incubated a few hours, and the maltose content after saccharification reaches desired value, and iodine inspection is qualified, obtains saccharification feed liquid.
5. enzyme according to claim 1 joins the preparation method of superhigh maltose syrup of three enzymatic conversion of raw material pre-treatment, its feature
It is：Saccharification feed liquid sterilizing described in step (4) is specifically, be saccharified with absolute ethyl alcohol in the obtained saccharification feed liquid of step (3)
Detection, in the presence of feed liquid is without dextrin, saccharifying tank is heated to 90 DEG C, and is incubated 1 hour, is then cooled to 60 DEG C, stands 2 hours,
Obtain sterile malt sugar material liquid.
6. enzyme according to claim 1 joins the preparation method of superhigh maltose syrup of three enzymatic conversion of raw material pre-treatment, its feature
It is：Sterile malt sugar material liquid removal of impurities described in step (5) is specifically, will be de- for sterile malt sugar material liquid input obtained in step (4)
Color kettle, and the adsorbent, flocculant is added, decolouring kettle temperature degree is quickly raised to 80 DEG C, after stirring 1 hour, and it is little to stand 4
When, impurity and flocculate are filtered by filter by the liquid in decolouring kettle, cleaning sterile maltose feed liquid is obtained.
7. enzyme according to claim 1 joins the preparation method of superhigh maltose syrup of three enzymatic conversion of raw material pre-treatment, its feature
It is：Cleaning sterile maltose feed liquid described in step (6) is purified specifically, throwing sterile malt sugar material liquid obtained in step (5)
Enter the absorption of resin anion (R.A.) adsorption tanks, then by absorption after feed liquid send into the absorption of resin cation adsorption tanks, through negative and positive from
After son absorption, pure maltose solution is obtained.
8. enzyme according to claim 1 joins the preparation method of superhigh maltose syrup of three enzymatic conversion of raw material pre-treatment, its feature
It is：Pure maltose feed liquid concentration described in step (7) specifically, by maltose solution input concentration kettle pure for step (6),
Discharge after water content is concentrated into less than 15%, obtain final product this high-load malt syrup.
9. enzyme according to claim 1 joins the preparation method of superhigh maltose syrup of three enzymatic conversion of raw material pre-treatment, its feature
It is：The adsorbent is the one or more kinds of mixtures in activated carbon, silica gel or zeolite molecular sieve.
10. enzyme according to claim 1 joins the preparation method of superhigh maltose syrup of three enzymatic conversion of raw material pre-treatment, its feature
It is：The flocculant is food grade polyacrylamide or the one or more kinds of mixtures in shitosan.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107557411A (en) * | 2017-10-30 | 2018-01-09 | 无锡甜丰食品有限公司 | A kind of preparation method of superhigh maltose syrup |
| CN109402198A (en) * | 2018-12-21 | 2019-03-01 | 山东省鲁洲食品集团有限公司 | A kind of preparation method that crisp sweets are syrup dedicated |
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| CN102559812A (en) * | 2012-01-20 | 2012-07-11 | 吉林农业大学 | Method for preparing maltose syrup by continuous saccharification of enzyme membrane reactor |
| CN102766666A (en) * | 2012-07-06 | 2012-11-07 | 上海晓乐东潮生物技术开发有限公司 | Preparation method for ultrahigh-content malt syrup |
| CN102899371A (en) * | 2012-10-30 | 2013-01-30 | 申德超 | Squeezing enzymolysis and saccharify method for producing ultrahigh malt syrup raw material |
| CN103695503A (en) * | 2013-12-30 | 2014-04-02 | 河南飞天农业开发股份有限公司 | Maltose syrup with high sugaring off temperature and preparation method of maltose syrup |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559812A (en) * | 2012-01-20 | 2012-07-11 | 吉林农业大学 | Method for preparing maltose syrup by continuous saccharification of enzyme membrane reactor |
| CN102766666A (en) * | 2012-07-06 | 2012-11-07 | 上海晓乐东潮生物技术开发有限公司 | Preparation method for ultrahigh-content malt syrup |
| CN102899371A (en) * | 2012-10-30 | 2013-01-30 | 申德超 | Squeezing enzymolysis and saccharify method for producing ultrahigh malt syrup raw material |
| CN103695503A (en) * | 2013-12-30 | 2014-04-02 | 河南飞天农业开发股份有限公司 | Maltose syrup with high sugaring off temperature and preparation method of maltose syrup |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107557411A (en) * | 2017-10-30 | 2018-01-09 | 无锡甜丰食品有限公司 | A kind of preparation method of superhigh maltose syrup |
| CN109402198A (en) * | 2018-12-21 | 2019-03-01 | 山东省鲁洲食品集团有限公司 | A kind of preparation method that crisp sweets are syrup dedicated |
| CN109402198B (en) * | 2018-12-21 | 2022-01-28 | 山东省鲁洲食品集团有限公司 | Preparation method of special syrup for crunchy candy |
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Application publication date: 20170315 |