CN102766666A - Preparation method for ultrahigh-content malt syrup - Google Patents
Preparation method for ultrahigh-content malt syrup Download PDFInfo
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- CN102766666A CN102766666A CN2012102342061A CN201210234206A CN102766666A CN 102766666 A CN102766666 A CN 102766666A CN 2012102342061 A CN2012102342061 A CN 2012102342061A CN 201210234206 A CN201210234206 A CN 201210234206A CN 102766666 A CN102766666 A CN 102766666A
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Abstract
The invention relates to a preparation method for ultrahigh-content malt syrup, which includes the following steps: (1) grain starch as material is added with water and produced into slurry, the dry matter concentration of the slurry is regulated to be 20 to 30 percent, the pH value of the slurry is regulated to be 5.8 to 6.2, and thermostable amylase is added into the slurry; (2) a cooker is used for cooking the slurry under 108 DEG C to 110 DEG C for 5 to 6 minutes, so that starch paste is obtained, the starch paste is liquefied to be hydrolyzed, the liquefaction time is 30 to 150 minutes, and after the temperature is increased to kill enzyme, aqueous maltodextrin solution is obtained; (3) the pH value of the aqueous maltodextrin solution is regulated to 5.0 to 5.2, the aqueous maltodextrin solution is added with diastatic enzyme, the bienzyme method or the trienzyme method is adopted to saccharify the aqueous maltodextrin solution, the saccharification time is 50 to 60 hours, and after the temperature is increased to kill enzyme, the ultrahigh-content malt syrup is obtained. According to the preparation method, low-DE value maltodextrin without needing filtration can be stably obtained, more than 85 percent of maltose can be obtained, and the method has a good application prospect.
Description
Technical field
The invention belongs to the preparation field of malt syrup, particularly a kind of preparation method of superelevation content malt syrup.
Background technology
To make high-purity maltose syrup in the prior art, at first improve the content of SANMALT-S in the saccharification liquid (DP2) itself as far as possible, secondly be exactly to reduce SANMALT-S in the malt syrup (DP2) monose and the oligosaccharide contg of composition in addition.Conclude existing research, below three kinds of methods the most concentrated: (1) adds a kind of even two kinds of debranching factors and beta-amylase acting in conjunction; (2) reduce liquefaction glucose equivalent (DE value) as far as possible; (3) add in the subsequent technique such as glucoamylase malt syrup is carried out subsequent disposal.Along with reaching its maturity of lytic enzyme preparation utilisation technology, what comparatively generally adopt is first method.The third method is only as the means of supplementing out economy, is intended to reduce the content of trisaccharide maltose and other oligose and dextrin in the saccharification liquid.
And second method general and the first method coupling, in manufacturing enterprise's practical application at present, Chang Yin the actually operating difficulty just occurs crossing low DE value (usually＜10), and liquefier viscosity is big, and saccharification speed is slow, the very difficult completion of reaction, filtration difficulty or the like.If can be from stable, the thorough angle lifting technology of jet cooking support liquefaction, the liquefier that makes full use of than low DE value improves malt syrup purity, then will be to have the best method of realistic meaning based on existing basic economy the most in the malt syrup technology.
Summary of the invention
Technical problem to be solved by this invention provides a kind of preparation method of superelevation content malt syrup; This method is stable to obtain low DE value maltodextrin and the filtration of worrying about nothing; Process superelevation SANMALT-S for enzymic hydrolysis optimum hydrolysis substrate condition is provided; Can obtain the SANMALT-S more than 85%, have a good application prospect.
The preparation method of a kind of superelevation content malt syrup of the present invention comprises:
(1) is raw material with the cereal starch, adds entry and process slurry that the dry substance concentration of slurry is transferred to 20% ~ 30%, and the pH value transfers to 5.8 ~ 6.2, in slurry, adds fire resistant alpha-diastase;
(2) use pulp digester to obtain starch paste in 108 ~ 110 ℃ of above-mentioned slurries of boiling after 5 ~ 6 minutes, to the starch paste hydrolysis of liquefying, liquefying time is 30 ~ 150min, and the enzyme that goes out that heats up obtains that DE value is not more than 10, the iodine examination is the maltodextrin aqueous solution that does not have blueness;
(3) above-mentioned maltodextrin pH value of water solution is transferred to 5.0 ~ 5.2, add saccharifying enzyme and adopt double-enzyme method or three enzyme process to carry out saccharification, saccharification time is 50 ~ 60h, and the enzyme that goes out that heats up promptly gets superelevation content malt syrup.
Cereal starch in the said step (1) is a W-Gum.
Pulp digester in the said step (2) is the jet cooking device with the adjustable shearing force in heating zone.
Pulp digester heating zone shearing force in the said step (2) is striden heating zone pressure by adjustment liquid material and is fallen and regulate.
Pulp digester heating zone in the said step (2) is striden heating zone pressure and is fallen and be at least 2 kilograms.
The add-on of the fire resistant alpha-diastase in the said step (1) is 0.3g/kg DS.
Saccharifying enzyme is beta-amylase and Pullulanase when adopting double-enzyme method in the said step (3).
Saccharifying enzyme is that beta-amylase, Pullulanase and SANMALT-S generate enzyme when adopting three enzyme process in the said step (3).
Beneficial effect
(1) the present invention is stable obtains low DE value maltodextrin and the filtration of worrying about nothing, and processes superelevation SANMALT-S for enzymic hydrolysis optimum hydrolysis substrate condition is provided; Rely on expensive zymin to promote SANMALT-S purity relatively separately, regulating measure is diversification more, also economic and reliable more;
(2) three enzyme process can be stablized and obtain content 90% above SANMALT-S, and may command glucose is below 4.5%; Double-enzyme method can obtain the SANMALT-S more than 85%, and the control glucose content has a good application prospect below 0.2%.
Description of drawings
Liquefier DE value and saccharification DP2 graph of a relation when Fig. 1 is three enzyme process saccharification;
Liquefier DE value and saccharification DP2 graph of a relation when Fig. 2 is the double-enzyme method saccharification.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Main raw of the present invention:
W-Gum: emerging trade edible corn starch, Binzhou Jin Hui corn development corporation, Ltd., product standard number: GB/T8885;
Ye Huamei: fire resistant alpha-diastase (profit can be come), Novi's letter, 4.5 ten thousand U/g;
Saccharifying enzyme: beta-amylase, outstanding person's ability section, 1230DP °;
Pullulanase (Promozyme D2), Novi's letter, 1350NPUN/g;
SANMALT-S generates enzyme (Maltogenase L), Novi's letter, 3200MANU/g.
Embodiment 1
(1) is raw material with the cereal starch, adds entry and process slurry that the dry substance concentration of slurry is transferred to 20%, and the pH value transfers to 5.8, in slurry, add 0.3g/kg DS fire resistant alpha-diastase;
(2) use pulp digester in 108 ℃ of above-mentioned slurries of boiling 5 minutes, digestion process confirms that pulp digester strides heating zone pressure difference (pulp digester raw material feed pressure-grog discharging pressure) and be not less than 2 kilograms.Obtain starch paste after the boiling, to the starch paste hydrolysis of liquefying, liquefying time is respectively 30,60,90,120 and 150min, and the gained liquefier is carried out the iodine examination, measures filtration velocity and DE value, and the result sees table 1, and the intensification enzyme that goes out promptly gets the maltodextrin aqueous solution;
(3) above-mentioned maltodextrin pH value of water solution is transferred to 5.0, add saccharifying enzyme and adopt double-enzyme method or three enzyme process to carry out saccharification, saccharification time is 50h, and the enzyme that goes out that heats up promptly gets superelevation content malt syrup, and HPLC detection assay maltose content is carried out in sampling.
Table 1 uses pulp digester boiling 28% W-Gum liquefaction back DE value and iodine examination color record
As above table, liquefier in liquefying time 120min, guarantee DE value 10 and iodine try to reach a standard.
Based on above-mentioned experimental result, 10 groups of DE value liquefiers that in liquefier DE value 4-13 interval, distribute carry out the malt amylase experiment, and the result of relatively two enzyme and three enzyme glycolysiss, see table 2.
Liquefier saccharification data between the table 2 DE value 4-13
Annotate: filtration velocity is measured and is adopted Φ 18cm qualitative filter paper.
Last table data presentation only between 4-5, still can keep good filtration velocity in liquefier DE value, explains that liquefier does not have insoluble macromole when extremely low DE value (hydrolysis degree is very low).DE value height when the examination of liquefier iodine reaches a standard, under identical Mashing process condition, directly related maltose equivalent, liquefier control is more thorough, accurate, and the DE value of liquefier was low more when the iodine examination reached a standard, and maltose equivalent is high more in the follow-up saccharification.From HPLC sugar spectrum analysis, can observe three enzyme process and can stablize and obtain content 90% above SANMALT-S, and may command glucose is at (Fig. 1) below 4.5%; Double-enzyme method can obtain the SANMALT-S more than 85%, and the control glucose content is at (Fig. 2) below 0.2%.
Can see that by Fig. 1 and 2 maltose equivalent presents linear relationship clearly after liquefaction DE value and the saccharification, no matter is three enzyme process or double-enzyme method, and the productive rate of SANMALT-S reduces with the intensification of the degree of liquefaction.
Claims (8)
1. the preparation method of a superelevation content malt syrup comprises:
(1) is raw material with the cereal starch, adds entry and process slurry that the dry substance concentration of slurry is transferred to 20% ~ 30%, and the pH value transfers to 5.8 ~ 6.2, in slurry, adds fire resistant alpha-diastase;
(2) use pulp digester to obtain starch paste in 108 ~ 110 ℃ of above-mentioned slurries of boiling after 5 ~ 6 minutes, to the starch paste hydrolysis of liquefying, liquefying time is 30 ~ 150min, and the enzyme that goes out that heats up obtains that DE value is not more than 10, the iodine examination is the maltodextrin aqueous solution that does not have blueness;
(3) above-mentioned maltodextrin pH value of water solution is transferred to 5.0 ~ 5.2, add saccharifying enzyme and adopt double-enzyme method or three enzyme process to carry out saccharification, saccharification time is 50 ~ 60h, and the enzyme that goes out that heats up promptly gets superelevation content malt syrup.
2. the preparation method of a kind of superelevation content malt syrup according to claim 1 is characterized in that: the cereal starch in the said step (1) is a W-Gum.
3. the preparation method of a kind of superelevation content malt syrup according to claim 1 is characterized in that: the add-on of the fire resistant alpha-diastase in the said step (1) is 0.3g/kg DS.
4. the preparation method of a kind of superelevation content malt syrup according to claim 1 is characterized in that: the pulp digester in the said step (2) is the jet cooking device with the adjustable shearing force in heating zone.
5. the preparation method of a kind of superelevation content malt syrup according to claim 4 is characterized in that: the pulp digester heating zone shearing force in the said step (2) is striden heating zone pressure by adjustment liquid material and is fallen and regulate.
6. the preparation method of a kind of superelevation content malt syrup according to claim 5 is characterized in that: the pulp digester heating zone in the said step (2) is striden heating zone pressure and is fallen and be at least 2 kilograms.
7. the preparation method of a kind of superelevation content malt syrup according to claim 1 is characterized in that: saccharifying enzyme is beta-amylase and Pullulanase when adopting double-enzyme method in the said step (3).
8. the preparation method of a kind of superelevation content malt syrup according to claim 1 is characterized in that: saccharifying enzyme is that beta-amylase, Pullulanase and SANMALT-S generate enzyme when adopting three enzyme process in the said step (3).
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| CN2012102342061A CN102766666A (en) | 2012-07-06 | 2012-07-06 | Preparation method for ultrahigh-content malt syrup |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103525787A (en) * | 2013-09-30 | 2014-01-22 | 淮北市三和诺生物工程有限责任公司 | Beer syrup enzyme and preparation method thereof |
| CN106498008A (en) * | 2016-11-30 | 2017-03-15 | 无锡甜丰食品有限公司 | The preparation method of superhigh maltose syrup of three enzymatic conversion of enzyme ginseng raw material pre-treatment |
| CN106520862A (en) * | 2016-11-30 | 2017-03-22 | 无锡甜丰食品有限公司 | Method for preparing high-content malt syrup by double enzymes saccharifying processing with raw material enzyme |
| CN106755176A (en) * | 2016-11-30 | 2017-05-31 | 无锡甜丰食品有限公司 | The method that maltodextrin is prepared with oat starch |
| CN107557411A (en) * | 2017-10-30 | 2018-01-09 | 无锡甜丰食品有限公司 | A kind of preparation method of superhigh maltose syrup |
| CN107586807A (en) * | 2017-10-30 | 2018-01-16 | 无锡甜丰食品有限公司 | A kind of collaboration preparation method of superhigh maltose syrup |
| CN107686853A (en) * | 2017-10-30 | 2018-02-13 | 无锡甜丰食品有限公司 | A kind of method that multi-enzyme method prepares superhigh maltose syrup |
| CN107739744A (en) * | 2017-10-30 | 2018-02-27 | 无锡甜丰食品有限公司 | A kind of filtering preparation method of malt syrup |
| CN108690858A (en) * | 2018-02-08 | 2018-10-23 | 河南科技学院 | The method for producing high maltose syrup using mPEG-Mal5000- beta amylases |
| CN110343729A (en) * | 2018-04-02 | 2019-10-18 | 无锡甜丰食品有限公司 | A kind of preparation method of low DE value glucose syrup |
| CN112210493A (en) * | 2020-09-17 | 2021-01-12 | 北京劢克机械工程有限公司 | Ultrahigh maltose syrup production system and preparation method thereof |
| WO2021138810A1 (en) * | 2020-01-07 | 2021-07-15 | Loren Chen | Malto-dextrin composition with low de value and low viscosity and method for making the same |
-
2012
- 2012-07-06 CN CN2012102342061A patent/CN102766666A/en active Pending
Non-Patent Citations (2)
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| 刘星等: "高麦芽糖浆研究进展", 《食品科技》 * |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103525787A (en) * | 2013-09-30 | 2014-01-22 | 淮北市三和诺生物工程有限责任公司 | Beer syrup enzyme and preparation method thereof |
| CN106498008A (en) * | 2016-11-30 | 2017-03-15 | 无锡甜丰食品有限公司 | The preparation method of superhigh maltose syrup of three enzymatic conversion of enzyme ginseng raw material pre-treatment |
| CN106520862A (en) * | 2016-11-30 | 2017-03-22 | 无锡甜丰食品有限公司 | Method for preparing high-content malt syrup by double enzymes saccharifying processing with raw material enzyme |
| CN106755176A (en) * | 2016-11-30 | 2017-05-31 | 无锡甜丰食品有限公司 | The method that maltodextrin is prepared with oat starch |
| CN107557411A (en) * | 2017-10-30 | 2018-01-09 | 无锡甜丰食品有限公司 | A kind of preparation method of superhigh maltose syrup |
| CN107586807A (en) * | 2017-10-30 | 2018-01-16 | 无锡甜丰食品有限公司 | A kind of collaboration preparation method of superhigh maltose syrup |
| CN107686853A (en) * | 2017-10-30 | 2018-02-13 | 无锡甜丰食品有限公司 | A kind of method that multi-enzyme method prepares superhigh maltose syrup |
| CN107739744A (en) * | 2017-10-30 | 2018-02-27 | 无锡甜丰食品有限公司 | A kind of filtering preparation method of malt syrup |
| CN108690858A (en) * | 2018-02-08 | 2018-10-23 | 河南科技学院 | The method for producing high maltose syrup using mPEG-Mal5000- beta amylases |
| CN108690858B (en) * | 2018-02-08 | 2021-12-07 | 河南科技学院 | Method for producing high maltose syrup by using mPEG-Mal 5000-beta-amylase |
| CN110343729A (en) * | 2018-04-02 | 2019-10-18 | 无锡甜丰食品有限公司 | A kind of preparation method of low DE value glucose syrup |
| WO2021138810A1 (en) * | 2020-01-07 | 2021-07-15 | Loren Chen | Malto-dextrin composition with low de value and low viscosity and method for making the same |
| CN115087742A (en) * | 2020-01-07 | 2022-09-20 | 陈乐仁 | Maltodextrin component with low DE value and low viscosity and preparation method thereof |
| US11549001B2 (en) | 2020-01-07 | 2023-01-10 | Loren Chen | Malto-dextrin composition with low DE value and low viscosity and method for making the same |
| CN115087742B (en) * | 2020-01-07 | 2023-10-24 | 陈乐仁 | Maltodextrin component with low DE value and low viscosity and its preparation method |
| CN112210493A (en) * | 2020-09-17 | 2021-01-12 | 北京劢克机械工程有限公司 | Ultrahigh maltose syrup production system and preparation method thereof |
| CN112210493B (en) * | 2020-09-17 | 2022-10-18 | 北京劢克机械工程有限公司 | Ultrahigh maltose syrup production system and preparation method thereof |
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Application publication date: 20121107 |