CN106497835B - One plant of lactobacillus fermenti for suppressing Streptococcus mutans propagation - Google Patents
One plant of lactobacillus fermenti for suppressing Streptococcus mutans propagation Download PDFInfo
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- CN106497835B CN106497835B CN201610944860.XA CN201610944860A CN106497835B CN 106497835 B CN106497835 B CN 106497835B CN 201610944860 A CN201610944860 A CN 201610944860A CN 106497835 B CN106497835 B CN 106497835B
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- lactobacillus fermenti
- lactobacillus
- streptococcus mutans
- fermenti
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- 241000186840 Lactobacillus fermentum Species 0.000 title claims abstract description 42
- 241000194019 Streptococcus mutans Species 0.000 title abstract description 16
- 238000004321 preservation Methods 0.000 claims abstract description 5
- 230000000694 effects Effects 0.000 claims abstract description 3
- 150000004676 glycans Chemical class 0.000 claims description 16
- 229920001282 polysaccharide Polymers 0.000 claims description 16
- 239000005017 polysaccharide Substances 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000008103 glucose Substances 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000005119 centrifugation Methods 0.000 claims description 4
- 229940012969 lactobacillus fermentum Drugs 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 235000021107 fermented food Nutrition 0.000 claims description 2
- 229940034610 toothpaste Drugs 0.000 claims description 2
- 239000000606 toothpaste Substances 0.000 claims description 2
- 239000000022 bacteriostatic agent Substances 0.000 claims 1
- 235000019985 fermented beverage Nutrition 0.000 claims 1
- 235000010335 lysozyme Nutrition 0.000 abstract description 7
- 230000001580 bacterial effect Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 102000016943 Muramidase Human genes 0.000 description 4
- 108010014251 Muramidase Proteins 0.000 description 4
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 239000004325 lysozyme Substances 0.000 description 4
- 229960000274 lysozyme Drugs 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000000214 mouth Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 241000289669 Erinaceus europaeus Species 0.000 description 1
- 229920002444 Exopolysaccharide Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000896292 Odontothrips loti Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 235000020167 acidified milk Nutrition 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000001013 cariogenic effect Effects 0.000 description 1
- 229940112822 chewing gum Drugs 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 125000005909 ethyl alcohol group Chemical group 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/364—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
- A23G3/366—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins containing microorganisms, enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/143—Fermentum
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
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Abstract
The invention discloses the lactobacillus fermenti that one plant suppresses Streptococcus mutans propagation, belong to microbial technology field.The lactobacillus fermenti of the present invention is preserved in China typical culture collection center on the 22nd in August in 2016, and deposit number is CCTCC No:M 2016424, preservation address are Wuhan, China, and Wuhan University, is categorized as lactobacillus fermenti Y29.The bacterial strain can effectively suppress Streptococcus mutans, and be resistant to 1.2mg/mL lysozymes, have the effect of prevention dental decayed tooth.
Description
Technical field
The present invention relates to the lactobacillus fermenti that one plant suppresses Streptococcus mutans propagation, belong to microbial technology field.
Background technology
Streptococcus mutans (Streptococcus mutans) are one of main cariogenic hedgehog fungus in oral cavity, are common in plaque
In, the generation of carious tooth can be caused by being metabolized the acidic materials produced.The lactobacillus fermenti in cavity source can suppress the chain that makes a variation
The propagation of coccus.Therefore the amount for reducing Streptococcus mutans in oral cavity is that we reduce one of effective measures of carious tooth.
The content of the invention
It is an object of the invention to provide the lactobacillus fermenti that one plant suppresses Streptococcus mutans propagation, in August, 2016
China typical culture collection center is preserved within 22nd, deposit number is CCTCC No:M 2016424, preservation address are China
Wuhan, Wuhan University, is categorized as lactobacillus fermenti Y29.
Second object of the present invention is to provide the cultural method of the lactobacillus fermenti Y29, and the method is to ferment
Lactobacillus Y29 is seeded in MRS culture mediums, and 18h is cultivated in 37 DEG C.
Third object of the present invention is to provide the exocellular polysaccharide of lactobacillus fermenti production, the exocellular polysaccharide be by
Lactobacillus fermenti Y29 is connected in the MRS culture mediums of the glucose containing 80~100g/L, and 37 DEG C of 20~48h of culture, collect polysaccharide.
Fourth object of the present invention is to provide a kind of method for producing exocellular polysaccharide, is to be connected to the lactobacillus fermenti
In the MRS culture mediums of the glucose containing 80~100g/L, 37 DEG C of 20~48h of culture.
The 5th purpose of the present invention is to provide the application of the lactobacillus fermenti Y29.
In one embodiment of the invention, the application includes：Antibacterial microbial inoculum is prepared, prepares fermented food, fermentation
Drink chewing gum, pressed candy etc..
The 6th purpose of the present invention is to provide a kind of toothpaste with oral bacteriostasis effect, contains the lactobacillus fermenti
Y29 active ingredients.
Beneficial effect：Lactobacillus fermenti Y29 provided by the invention, has the characteristic of extracellular polysaccharide, the production of exocellular polysaccharide
Measure as 3.34g/L, can effectively suppress the propagation of Streptococcus mutans, and be resistant to 1.2mg/mL lysozymes.
Biomaterial preservation
Lactobacillus fermenti (Lactobacillusfermentum) Y29, is preserved in Chinese Typical Representative on 22nd in August in 2016
Culture collection, deposit number are CCTCC No:M 2016424, preservation address are Wuhan, China, Wuhan University.
Brief description of the drawings
Fig. 1 is gel electrophoresis figure after lactobacillus fermenti bacterium colony PCR；M, Maker DL1000MT；1~12, respectively numbering 1
~12 strain to be tested；
Fig. 2 is suppression circle of the different material to Streptococcus mutans；1, lactobacillus fermenti；2, sterile water；3, lactic acid.
Embodiment
The detection of exocellular polysaccharide：(1) drafting of standard curve：Using water as blank control, respectively draw 0.2,0.4,0.6,
0.8th, 1.0,1.2,1.4, the glucose that 1.6mL concentration is 4g/L, distilled water are mended to 2mL.It is 6% (w/v) to add 1.0mL concentration
Phenol, the static 10min of concentrated sulfuric acid 5.0mL, shake up room temperature place 20min, 490nm survey light absorption value.(2) zymotic fluid detects：Take
1mL Exopolysaccharide Production From The Fermentation liquid adds 1mL distilled water, adds the phenol mixing that 1.0mL concentration is 6% (w/v) and is then added to the dense sulphur of 5.0mL
In acid, static 10min, shakes up, and room temperature places 20min, and 490nm surveys light absorption value.
The screening of 1 lactobacillus fermenti Y29 of embodiment
(1) screen
From 7 adult's oral plaque samplings, sterile saline dilution applies tablet, chooses single bacterium and carry out bacterium colony PCR, specifically
Property primer sequence：Lactobacillusfermentum
F\R:TGAAGGAATCGTGGTCGGTG AAACTGGGTCAAGAAGCGGA, 224bp is by Fig. 1 for purpose fragment length
Bacterial strain 1-10 clip sizes are known between 200-300bp, and bacterial strain 1-5 sizes are closer to purpose fragment length (224bp), therefore
The bacterial strain of primary dcreening operation selection numbering 1-5 carries out further identification and analysis.
(2) identify
Primary dcreening operation bacterial strain is rule to obtain single bacterium, in 37 DEG C of quiescent culture 24h in MRS fluid nutrient mediums, collects thalline, extraction
Genome, using universal primer carry out 16s rDNA amplifications, its sequence as shown in SEQ ID NO.1, in ncbi database into
Row sequence alignment, by strain was named lactobacillus fermenti Y29.
The bacteriostasis of 2 lactobacillus fermenti Y29 of embodiment
Lactobacillus fermenti is rule and cultivates 18h, single bacterium is chosen and is inoculated in MRS culture mediums, 37 DEG C, cultivates 16h.Then press
4% inoculum concentration be inoculated in production antibacterial substance liquid fermentation medium in (medium component MRS 52.7g, glucose 1g, spit
Warm 201g, MgSO40.75g, distilled water 1L), after 37 DEG C are cultivated 20h, 12000r/min, 4 DEG C of centrifugation 5min take supernatant, through 0.22
μm membrane filtration is placed on 4 DEG C of refrigerators and preserves.
By cultured indicator bacteria Streptococcus mutans (Streptococcus mutans) CGMCC 1.2499, (bacterium is dense to be
107CFU/mL) 1 is pressed with soft agar BHI culture mediums:100 (bacterium solutions：Soft agar medium) volume ratio mix after be poured over and be placed with
On the bottom element agar culture medium of Oxford cup.Oxford cup is taken out after to be solidified, 100 μ L acidified milks are separately added into corresponding hole
Bacillus Y29 fermented supernatant fluids, sterile water and lactic acid, then tablet is placed in 37 DEG C of culture 18-24h, observe inhibition zone.As a result show
Show, lactobacillus fermenti Y29 16.2 ± 0.3mm of antibacterial circle diameter, to Streptococcus mutans (Streptococcus mutans)
CGMCC1.2499 has significant inhibitory action.
3 lactobacillus fermenti Y29 of embodiment produces antibacterial exocellular polysaccharide
By inoculation to extracellular polysaccharide fluid nutrient medium, (component is the MRS containing final concentration of 10% (w/v) glucose
Culture medium) in, 30 DEG C of culture 48h.10mL nutrient solutions are taken, add 2mL 80% (w/v) trichloroacetic acid, ice bath stirring 30min.4
DEG C, 4100r/min centrifugations 20min removes thalline and albumen, 3 times of volume frost absolute ethyl alcohols is added into supernatant, 4 DEG C refrigerated
Night, polysaccharide are separated out in flocculent deposit.4 DEG C, 4000r/min centrifugation 20min, dissolve polysaccharide precipitation, 4 DEG C standby with 10mL distilled water
With.After testing, the yield of lactobacillus fermenti exocellular polysaccharide is 3.34g/L.
Tolerances of the 4 lactobacillus fermenti Y29 of embodiment to lysozyme
Tolerance situation of the lactobacillus to lysozyme is investigated using Odontothrips loti.Lower floor's culture medium be 15g/L agar, upper strata
For the MRS culture mediums and lactobacillus fermenti Y29 (10 of 7g/L7CFU/mL mixture).Using Oxford cup in the culture medium of upper strata
Punching, then adds the lysozyme soln (0.2-3.0mg/mL) of the various concentrations of 100 μ L in each hole.By above-mentioned tablet
At 37 DEG C, 5%CO2Overnight incubation, the tolerance according to the situation analysis Bacillus acidi lactici of inhibition zone to lysozyme.The results show that
Lactobacillus fermenti Y29 is resistant to 1.2mg/mL lysozymes.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various change and modification, therefore the protection model of the present invention
Enclosing be subject to what claims were defined.
SEQUENCE LISTING
<110>Southern Yangtze University
<120>One plant of lactobacillus fermenti for suppressing Streptococcus mutans propagation
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 1497
<212> DNA
<213> Lactobacillus fermentum Y29
<400> 1
ggaccgggcg ggttgcctaa taatgcagtc gaacgcgttg gcccaattga ttgatggtgc 60
ttgcacctga ttgattttgg ttgccaacga gtggcggacg ggtgagtaac acgtaggtaa 120
cctgcccaga agcgggggac aacatttgga aacagatgct aataccgcat aacagcgttg 180
ttcgcatgaa caacgcttaa aagatggctt ctcgctatca cttctggatg gacctgcggt 240
gcattaggct tgttggtggg gtaacggcct accaagrcga tgatgcatag ccgagttgag 300
gagactgatc gkccacaatk ggactgagac acgrcccata ctcctacggg aggcagcagt 360
agggaatctt ccacaatggg cgcaagsctg atggagcaac accgcgtgag tgaagaaggg 420
tttcggctcg taaagctctg ttgttaaaga agaacacgta tgagagtaac tgttcatacg 480
ttgacggtat ttaaccagaa agtcacggct aactacgtgc cagcagccgc ggtaatacgt 540
aggtggcaag cgttatccgg atttattggg cgtaaagaga gtgcaggcgg ttttctaagt 600
ctgatgtgaa agcctttcgg cttaaccgga gaagtgcatc ggaaactgga taacttgagt 660
gcagaagagg gtagtggaac tccatgtgta gcggtggaat gcgtagatat atggaagaac 720
accagtggcg aaggcggcta cctggtctgc aactgacgct gagactcgaa agcatgggta 780
gcgaacagga ttagataccc tggtagtcca tgccgtaaac gatgagtgct aggtgttgga 840
gggtttccgc ccttcagtgc cggagctaac gcattaagca ctccgcctgg ggagtacgac 900
cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcggtgga gcatgtggtt 960
taattcgaag ctacgcgaag aaccttacca ggtcttgaca tcttgcgcca accctagaga 1020
tagggcgttt cccttcggga acgcaatgac aggtggtgca tggttcgtcg tcagctcgtg 1080
tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc ttgttactag ttgccagcat 1140
taagttgggc acctctagtg aagactgccg gtgacwaacc ggargmaggg tggggacgac 1200
gtcagatcat catgcycctt atgacctggg cttacmcacg tgcctaacaa tggaaccgga 1260
tgacaacgag tcgcgaactc gcgagggcaa gcaaatctct taaaaccgtt ctcagttcgg 1320
actgcaggct gcaactcgcc tgcacgaagt cggaatcgct agtaatcgcg gatcagcatg 1380
ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccatg agagtttgta 1440
acacccaaag tcggtggggt aaccttttag gagccagccg cctaaggtgg accgggg 1497
<210> 2
<211> 20
<212> DNA
<213>Artificial sequence
<400> 2
tgaaggaatc gtggtcggtg 20
<210> 3
<211> 20
<212> DNA
<213>Artificial sequence
<400> 3
aaactgggtc aagaagcgga 20
Claims (6)
1. one plant of lactobacillus fermenti (Lactobacillus fermentum) Y29, is preserved in China on 22nd in August in 2016
Type Tissue Collection, deposit number are CCTCC No:M 2016424, preservation address is Chinese, Wuhan, Wuhan University.
2. the cultural method of lactobacillus fermenti Y29 described in claim 1, it is characterised in that the method is by lactobacillus fermenti
Y29 is seeded in MRS culture mediums, and 6~24h is cultivated in 37 DEG C.
3. the exocellular polysaccharide that lactobacillus fermenti Y29 described in claim 1 is produced, the exocellular polysaccharide is by described in claim 1
Lactobacillus fermenti Y29 is seeded in the MRS culture mediums of the glucose containing 80~100g/L, 37 DEG C of 20~48h of culture, centrifugation, from upper
Polysaccharide is collected in clear liquid.
A kind of 4. method for producing exocellular polysaccharide, it is characterised in that be seeded to the lactobacillus fermenti Y29 described in claim 1
In the MRS culture mediums of the glucose containing 80~100g/L, 37 DEG C of 20~48h of culture.
5. lactobacillus fermenti Y29 is preparing bacteriostatic agent described in claim 1, the application in fermented food, fermented beverage is prepared.
6. a kind of toothpaste with oral bacteriostasis effect, it is characterised in that live containing lactobacillus fermenti Y29 described in claim 1
Property component.
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